CN105030889B - A kind of Sophora alopecuroide oral solution and preparation method thereof - Google Patents
A kind of Sophora alopecuroide oral solution and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of Sophora alopecuroide oral solutions and preparation method thereof, and oral solution is prepared by method comprising the following steps: Step 1: frying: by the seed of Sophora alopecuroide, carrying out frying processing；Step 2: extracting: the good Sophora alopecuroide of frying being crushed, after sieving, adds water to cook extraction 2-3 times, combined extract after extracting solution is cooled to 40 DEG C or less, is stood, then filtrate is concentrated, obtains concentrate by filtering；Step 3: alcohol precipitation: dehydrated alcohol being added into gained concentrate, is then cooled to 0-10 DEG C of standing, filters, after filtrate removes ethyl alcohol, adjusts pH value to 4.5-6.0, obtains medical fluid；Step 4: adjusting molten, filling and sterilizing: it is filling after water adjustment liquor strength is added into resulting medical fluid, it sterilizes to get oral solution.Oral solution of the present invention, having does not influence chick amount of drinking water and feed intake, and effective component matrine content is high, and treatment chick mycotic ingluvitis, proventriculitis, ventriculitis effect are obvious.
Description
Technical field
The invention belongs to technical field of Chinese veterinary medicine, and in particular to a kind of Sophora alopecuroide oral solution and preparation method thereof.
Background technique
At 30-35 DEG C, relative humidity 70%-80% is particularly suitable for mould the temperature of the chick growth environment of 1-14 age in days
The growth and propagation of bacterium, and chick defence capability is weak, vulnerable to fungus attack, mycotic ingluvitis, proventriculitis, ventriculitis occurs.
The chemicals such as a large amount of antibiotic and glandula digestive antiperspirant need to be fed by treating the disease, to press down mycostatic growth, and these
Drug side-effect is serious, and the normal feeding and drinking-water for often influencing chick are severely impacted the production performance of chick, and
These chemicals are difficult to be metabolized, and due to the accumulation of food chain, also constitute potential threat to the food safety of the mankind.Therefore it opens
It issues that natural safety, toxic side effect are small, does not influence normal feeding and the drinking-water of chick, and can effectively treat mycotic crop
Inflammation, proventriculitis, ventriculitis drug seem most important.
Summary of the invention
The purpose of the present invention is to provide a kind of Sophora alopecuroide oral solution and preparation method thereof, natural safety, toxic side effect
It is small, chick amount of drinking water is not influenced, does not influence feed intake, can be used for treating chick mycotic ingluvitis, proventriculitis, ventriculitis.
To achieve the goals above, the technical solution adopted by the present invention is as follows:
A kind of Sophora alopecuroide oral solution, is prepared by method comprising the following steps:
Step 1: frying: by Sophora alopecuroide, carrying out frying processing；
Step 2: extracting: the good Sophora alopecuroide of frying described in step 1 is crushed, after sieving, adds water to cook extraction 2-3 times,
Combined extract after extracting solution is cooled to 40 DEG C or less, stands, is separated by solid-liquid separation, then supernatant is concentrated, must be concentrated
Liquid；
Step 3: alcohol precipitation: dehydrated alcohol is added into concentrate obtained by step 2, is cooled to 0-10 DEG C of standing, solid-liquid point
From rear, supernatant removes ethyl alcohol, then adjusts pH value to 4.5-6.0 to get medical fluid；
Step 4: adjusting molten, filling and sterilizing: filling after the concentration of three gained medical fluid of set-up procedure, sterilizing is to get oral
Liquid.
As a further improvement of the present invention, Sophora alopecuroide described in step 1 is the seed of Sophora alopecuroide, used in the frying
Temperature is 140 DEG C -190 DEG C, and the frying time is 5min.
As a further improvement of the present invention, the sieve used of sieving described in step 2 is 20 meshes；The amount that water is added
Add water 8-12mL for every 1g Sophora alopecuroide；Described to decoct 100 DEG C of temperature extracted, the number for decocting extraction is 3 times, decocts mention every time
The time taken is 20-40min；Described to be separated by solid-liquid separation to be separated by filtration, the filter cloth filtered using 500 mesh is described dense
The degree of contracting is to concentrate relative density 1.20.
As a further improvement of the present invention, the amount that dehydrated alcohol is added described in step 3 is the quality of the ethyl alcohol into solution
Concentration is 40%-60%；The temperature being cooled to is 4 DEG C；It is described to be separated by solid-liquid separation to be separated by filtration, the filtering using
The filter cloth of 500 mesh.
As a further improvement of the present invention, adjustment liquor strength to every 1mL medical fluid described in step 4 is equivalent to Sophora alopecuroide
Raw medicinal herbs 1g；It is described filling using Brown Glass Brown glass bottles and jars only；The temperature of the sterilizing is 121 DEG C, and the time of sterilizing is 15min.
A kind of preparation method of Sophora alopecuroide oral solution, specifically includes the following steps:
Step 1: frying: by Sophora alopecuroide, carrying out frying processing；
Step 2: extracting: the good Sophora alopecuroide of frying described in step 1 is crushed, after sieving, adds water to cook extraction 2-3 times,
Combined extract after Sophora alopecuroide is cooled to 40 DEG C or less, stands, is separated by solid-liquid separation, then supernatant is concentrated, must be concentrated
Liquid；
Step 3: alcohol precipitation: dehydrated alcohol is added into concentrate obtained by step 2, is cooled to 0-10 DEG C of standing, solid-liquid point
From rear, supernatant removes ethyl alcohol, then adjusts pH value to 4.5-6.0 to get medical fluid；
Step 4: adjusting molten, filling and sterilizing: filling after the concentration of three gained medical fluid of set-up procedure, sterilizing is to get oral
Liquid.
A kind of Sophora alopecuroide oral solution preparation treatment chick mycotic ingluvitis, proventriculitis, ventriculitis drug in answer
With.
In the present invention:
Sophora alopecuroide is the seed of Sophora alopecuroide, and taste bitter and cold is slightly poisonous, has effects that heat-clearing and damp-drying drug, desinsection, stop dysentery.It is existing
Show for pharmacology: containing matrine, oxymatrine, sophocarpine, N-Oxysophocarpine, sophoramine, Sophoridine, Lai Man in Sophora alopecuroide
Alkali, the alkaloids such as aloperine with anti-inflammatory, anticancer, antiallergic action, anti-arrhythmia cordis, antiulcer, increasing leukocyte, are put down
Asthma, antipyretic, desinsection, calmness, analgesia, it is antiviral the effects of.
Compared with prior art, obtained by the present invention to have the beneficial effect that:
The present invention by the seed of Sophora alopecuroide in 140 DEG C of -190 DEG C of frying 5min, until skin crack, there is burnt odor taste；It can be effective
The toxicity for removing polar big bitter substance and Sophora alopecuroide can be for chicken after the oral solution obtained by this step process is watered
Group freely drinks, and the drinking-water and feed intake of chick will not be influenced because of the toxicity of Sophora alopecuroide and bitter taste；And through the step process
Afterwards, the extraction switching-out rate of the alkaloids such as active constituent matrine in Sophora alopecuroide can be effectively improved, to significantly improve obtained
The content of active constituent matrine in oral solution.
The present invention adopts the concentrate after extraction and carries out alcohol precipitation processing, has effectively removed the interference component for influencing drug effect, and
The content for not reducing active constituent matrine makes the oral solution of preparation in treatment chick mycotic ingluvitis, proventriculitis, ventriculitis
On effect it is more significant.
Oral solution of the present invention has determined Control of drug quality method, and every 1mL medical fluid is equivalent to Sophora alopecuroide raw medicinal herbs
1g, and it has been determined that the application dose when treating chick mycotic ingluvitis, proventriculitis, ventriculitis is that every 1mL oral solution is watered
0.2kg is freely drunk for chicken group.
Sophora alopecuroide oral solution obtained by the present invention is drunk for chicken group, does not influence chick amount of drinking water and feed intake, can be effective
Treat chick mycotic ingluvitis, proventriculitis, ventriculitis.
Specific embodiment
Further details of narration is carried out to the present invention with reference to embodiments.
Embodiment 1
Step 1: frying: the seed 100g of Sophora alopecuroide is weighed, in 170 DEG C of frying 5min；Skin crack at this time has burnt odor
Taste；
Step 2: extracting: the good Sophora alopecuroide of frying described in step 1 being crushed, after crossing 20 meshes, is added by 1g Sophora alopecuroide
Water 1000mL is added in the amount of 10mL water, decocts in 100 DEG C of boiling water and extracts 3 (Sophora alopecuroide and water in each extraction process
Amount is that 1g Sophora alopecuroide corresponds to 10mL water), each 30min；Merge extracting solution three times, extracting solution is cooled to 40 DEG C or less
Afterwards, it stands, crosses 500 mesh filter clothes, the relative density for then concentrating the filtrate to filtrate is 1.20, obtains concentrate (100mL)；
Step 3: alcohol precipitation: dehydrated alcohol mass concentration of ethyl alcohol into solution, which is added, into concentrate obtained by step 2 is
50%, at this point, the total amount that dehydrated alcohol is added is 100mL, 4 DEG C of standings are then cooled to, cross 500 mesh filter clothes, filtrate removes second
Alcohol adjusts pH value to 5.0, obtains medical fluid to no alcohol taste；
Step 4: adjusting molten, filling and sterilizing: it is 100mL that water to medicine liquid volume is added into the resulting medical fluid of step 3, this
When liquor strength be every 1mL medical fluid be equivalent to Sophora alopecuroide raw medicinal herbs 1g, 121 DEG C sterilizing 15mins filling with Brown Glass Brown glass bottles and jars only, i.e.,
Obtain Sophora alopecuroide oral solution.
Embodiment 2
Step 1: frying: the seed 100g of Sophora alopecuroide is weighed, in 180 DEG C of frying 5min；Skin crack at this time has burnt odor
Taste；
Step 2: extracting: the good Sophora alopecuroide of frying described in step 1 being crushed, after crossing 20 meshes, is added by 1g Sophora alopecuroide
Water 1000mL is added in the amount of 10mL water, decocts in 100 DEG C of boiling water and extracts 3 (Sophora alopecuroide and water in each extraction process
Amount is that 1g Sophora alopecuroide corresponds to 10mL water), each 30min；Merge extracting solution three times, extracting solution is cooled to 40 DEG C or less
Afterwards, it stands, crosses 500 mesh filter clothes, the relative density for then concentrating the filtrate to filtrate is 1.20, obtains concentrate (100mL)；
Step 3: alcohol precipitation: dehydrated alcohol mass concentration of ethyl alcohol into solution, which is added, into concentrate obtained by step 2 is
50%, at this point, the total amount that dehydrated alcohol is added is 100mL, 4 DEG C of standings are then cooled to, cross 500 mesh filter clothes, filtrate removes second
Alcohol adjusts pH value to 5.5, obtains medical fluid to no alcohol taste；
Step 4: adjusting molten, filling and sterilizing: it is 100mL that water to medicine liquid volume is added into the resulting medical fluid of step 3, this
When liquor strength be every 1mL medical fluid be equivalent to Sophora alopecuroide raw medicinal herbs 1g, 121 DEG C sterilizing 15mins filling with Brown Glass Brown glass bottles and jars only, i.e.,
Obtain Sophora alopecuroide oral solution.
Embodiment 3
The quality control standard of the Sophora alopecuroide oral solution as made from embodiment 1 or embodiment 2 are as follows: every 1mL medical fluid is equivalent to
Sophora alopecuroide raw medicinal herbs 1g.
Embodiment 4
The Sophora alopecuroide oral solution application dose as made from embodiment 1 or embodiment 2 is that every 1mL oral solution is watered 0.2kg confession
Chicken group freely drinks.
Comparative example 1
Step 1: extracting: weighing the seed 100g of Sophora alopecuroide, crushes, and after crossing 20 meshes, 10mL water is added by 1g Sophora alopecuroide
Amount, water 1000mL is added, is decocted in 100 DEG C of boiling water and extracts that 3 times (amount of Sophora alopecuroide and water is 1g in each extraction process
Sophora alopecuroide corresponds to 10mL water), each 30min；Merge extracting solution three times, after extracting solution is cooled to 40 DEG C or less, stands, mistake
500 mesh filter clothes, the relative density for then concentrating the filtrate to filtrate is 1.20, obtains concentrate (60mL)；
Step 2: adjusting molten, filling and sterilizing: after the pH of the resulting concentrate of set-up procedure one is 5.5, water being added thereto
It is 100mL to volume, liquor strength is that every 1mL medical fluid is equivalent to Sophora alopecuroide raw medicinal herbs 1g at this time, it is filling with Brown Glass Brown glass bottles and jars only,
121 DEG C of sterilizing 15min are to get Sophora alopecuroide oral solution.
Comparative example 2
Step 1: extracting: weighing the seed 100g of Sophora alopecuroide, crush, after crossing 20 meshes, add water 10mL's by 1g Sophora alopecuroide
Water 1000mL is added in amount, and extraction 3 times is decocted in 100 DEG C of boiling water, and (amount of Sophora alopecuroide and water is 1g hardship in each extraction process
Bean or pea correspond to 10mL water), each 30min；Merge extracting solution three times, after extracting solution is cooled to 40 DEG C or less, stands, mistake
500 mesh filter clothes, the relative density for then concentrating the filtrate to filtrate is 1.20, obtains concentrate (60mL)；
Step 2: alcohol precipitation: dehydrated alcohol mass concentration of ethyl alcohol into solution, which is added, into concentrate obtained by step 1 is
50%, at this point, the total amount that dehydrated alcohol is added is 60mL, 4 DEG C of standings are then cooled to, cross 500 mesh filter clothes, filtrate removes ethyl alcohol
To no alcohol taste, pH value is adjusted to 5.5, obtains medical fluid；
Step 3: adjusting molten, filling and sterilizing: it is 100mL that water to medicine liquid volume is added into the resulting medical fluid of step 2, this
When liquor strength be every 1mL medical fluid be equivalent to Sophora alopecuroide raw medicinal herbs 1g, 121 DEG C sterilizing 15mins filling with Brown Glass Brown glass bottles and jars only, i.e.,
Obtain Sophora alopecuroide oral solution.
Comparative example 3
Step 1: frying: weighing the seed 100g, 180 DEG C of frying 5min of Sophora alopecuroide；Skin crack at this time has burnt odor taste；
Step 2: extracting: the good Sophora alopecuroide of frying described in step 1 being crushed, after crossing 20 meshes, is added by 1g Sophora alopecuroide
Water 1000mL is added in 10mL, decocts in 100 DEG C of boiling water and extracts that 3 times (amount of Sophora alopecuroide and water is pressed in each extraction process
1g Sophora alopecuroide corresponds to 10mL water), each 30min；Merge extracting solution three times, after extracting solution is cooled to 40 DEG C or less, stand,
500 mesh filter clothes are crossed, the relative density for then concentrating the filtrate to filtrate is 1.20, obtains concentrate (100mL)；
Step 4: adjusting molten, filling and sterilizing: the pH of the resulting concentrate of set-up procedure two is 5.5, at this time the body of concentrate
Product is not required to that its volume of water is added to be 100mL, and liquor strength at this time is that every 1mL medical fluid is equivalent to Sophora alopecuroide raw medicinal herbs 1g, uses
Brown Glass Brown glass bottles and jars only is filling, and 121 DEG C of sterilizing 15min are to get Sophora alopecuroide oral solution.
Effect example 1
Carry out the quantitative determination of effective component matrine respectively to embodiment 1-2 and the obtained oral solution of comparative example 1-3,
It the results are shown in Table 1.
Detection method: high performance liquid chromatography.
Detecting instrument: Shimadzu high performance liquid chromatograph.
Matrine standard items: it is purchased from Nat'l Pharmaceutical & Biological Products Control Institute.
Chromatographic condition: chromatographic column: octadecylsilane chemically bonded silica column；Mobile phase: acetonitrile: water (is containing volumetric concentration
0.04% triethylamine, with phosphoric acid tune pH to 6.5)=20: 80；Flow velocity: 0.8mL/min；Column temperature: room temperature；Detector: ultraviolet detection
Device；Detection wavelength: 215nm；Sample volume: 10 μ L.
Reference substance solution preparation: the reference substance that every 1mL 50 μ g containing matrine is made with methanol dilution matrine standard items is molten
Liquid.
Sample solution preparation: taking oral solution 5mL, is separately added into 50% ethyl alcohol to 50mL, filtering takes subsequent filtrate 1mL to add first
Alcohol does reference substance solution to 10mL.
The content of matrine in 1 embodiment 1-2 of table and the obtained oral solution of comparative example 1-3
| Embodiment 1 | Embodiment 2 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| Content (mg/mL) | 18.79 | 19.22 | 2.75 | 2.52 | 19.01 |
The Sophora alopecuroide oral solution as made from the method for 1-2 of the embodiment of the present invention, the content of effective component matrine are greater than
15mg/mL (is expressed as 1.5% with percentage composition), with matrine in Sophora alopecuroide oral solution made from method in use comparative example 3
Content it is suitable, but be significantly higher than method used by comparative example 1 and comparative example 2 preparation Sophora alopecuroide oral solution, it is possible thereby to
Illustrate, Sophora alopecuroide can significantly improve the recovery rate of matrine in Sophora alopecuroide after frying is handled；And alcohol precipitation processing does not cause bitter beans
The loss of active constituent matrine in sub- oral solution.
2 functional experiment of effect example
It is using oral solution made from embodiment 1-2 as two groups of one group of experiment and experiment, comparative example 1-3 is obtained
Oral solution is respectively as three groups of one group of control, two groups of control, control carry out effect tests.In addition, also set up healthy control group,
Morbidity not treatment group and chemicals control group, carry out Contrast on effect.
Test material:
Experimental animal: 1 age in days male and female of Bai Yushuan A broiler chicks fifty-fifty 1600,8 groups, every group 200 are randomly divided into.
Nystatin: Hubei Xin Chuyuan medication chemistry Co., Ltd, product batch number 201410003 are purchased from.
Ranitidine hydrochloride: Hubei Yuancheng Saichuang Technology Co., Ltd., product batch number 201410007 are purchased from.
Test method:
To 1 Japanese instar chickling adaptability observation 3 days, chicken group's health, feeding drinking-water was normally.Wherein healthy control group feeding is normal
Feed, through 32 DEG C, relative humidity 90% exposes down the moldy feed of mould case culture 4 days for remaining seven groups of feeding.Continuous feeding 4
It, there is typical diarrhea, diarrhea in chick, and feeding reduces symptom.There are mold colony, glandular stomach swelling, muscular stomach cutin in dissection crop
Film ulcer.Then, control group is not treated in morbidity: the group is without any treatment；Medication therapy groups: it is added in every 1kg drinking-water mould
100,000 unit of rhzomorph and ranitidine hydrochloride 2mg are freely drunk for chick.Test one group: by oral solution made from embodiment 1,
0.2kg is watered by every 1mL oral solution freely to drink for chick；Test two groups: by oral solution made from embodiment 2, by 1mL mouthfuls every
It takes liquid and is watered 0.2kg and freely drunk for chick；It compares one group: oral solution made from comparative example 1 is watered by every 1mL oral solution
0.2kg is freely drunk for chick；It compares two groups: oral solution made from comparative example 2 is watered 0.2kg for young bird by every 1mL oral solution
Chicken is freely drunk；It compares three groups: oral solution made from comparative example 3 being watered 0.2kg by every 1mL oral solution and is freely drunk for chick
With；In addition to above-mentioned difference, each experimental group is consistent with other raisings of control group and management condition.
Each test group and control group chick compare therapeutic effect after continuously feeding 4 days, count amount of drinking water, feed intake, morbidity
Number, rehabilitation number, death toll；Test result is shown in Table 2.
Each experimental group chick of table 2 continuously feeds amount of drinking water after 4 days, feed intake, number of falling ill, rehabilitation number, the statistics of death toll
Table
Compared using oral solution prepared by 1-2 of the embodiment of the present invention by data compared with the method institute using comparative example 1-3
Oral solution obtained, the amount of drinking water of chick, feed intake significantly improve, and recovery from illness number improves, death toll decline；And the embodiment of the present invention
Oral solution prepared by 1-2 is slightly better than the therapeutic effect of medication therapy groups.It is possible thereby to illustrate: Sophora alopecuroide is through frying processing and alcohol
Sophora alopecuroide oral solution chick mycotic ingluvitis, gland caused by treatment chick is because of mycotic infection can be dramatically increased after heavy processing
Therapeutic effect in gastritis, ventriculitis.
Alcohol precipitation processing not will cause the loss of active constituent matrine in oral solution not only, also effectively eliminate influence kuh-seng
The impurity of alkali drug effect, therefore, Sophora alopecuroide oral solution made from 1-2 of the embodiment of the present invention are significantly higher than comparison in terms of therapeutic effect
Sophora alopecuroide oral solution made from example 3.
It therefore deduces that: caused by oral solution obtained by the method for the present invention can be applied to treatment chick because of mycotic infection
Chick mycotic ingluvitis, proventriculitis, ventriculitis.
Embodiment described above is merely a preferred embodiment of the present invention, and the simultaneously exhaustion of the feasible implementation of non-present invention.It is right
For persons skilled in the art, any aobvious to made by it under the premise of without departing substantially from the principle of the invention and spirit and
The change being clear to should be all contemplated as falling within claims of the invention.
Claims (7)
1. a kind of Sophora alopecuroide oral solution, which is characterized in that prepared by method comprising the following steps:
Step 1: frying: by Sophora alopecuroide, carrying out frying processing；
Step 2: extracting: the good Sophora alopecuroide of frying described in step 1 being crushed, after sieving, extraction 2-3 times is added water to cook, merges
Extracting solution stands after extracting solution is cooled to 40 DEG C or less, is separated by solid-liquid separation, then supernatant is concentrated, obtains concentrate；
Step 3: alcohol precipitation: dehydrated alcohol is added into concentrate obtained by step 2, is cooled to 0-10 DEG C of standing, after separation of solid and liquid,
Supernatant removes ethyl alcohol, then adjusts pH value to 4.5-6.0 to get medical fluid；
Step 4: adjusting molten, filling and sterilizing: it is filling after the concentration of three gained medical fluid of set-up procedure, it sterilizes to get oral solution.
2. a kind of Sophora alopecuroide oral solution according to claim 1, which is characterized in that Sophora alopecuroide described in step 1 is bitter beans
The seed of son, temperature used in the frying are 140 DEG C -190 DEG C, and the frying time is 5min.
3. a kind of Sophora alopecuroide oral solution according to claim 1, which is characterized in that sieving sieve used described in step 2 is
20 meshes；The amount that water is added is that every 1g Sophora alopecuroide adds water 8-12mL；It is described to decoct 100 DEG C of temperature extracted, it decocts and extracts
Number be 3 times, every time decoct extract time be 20-40min；Described to be separated by solid-liquid separation to be separated by filtration, the filtering uses
Be 500 mesh filter cloth, the degree of the concentration is to concentrate relative density 1.20.
4. a kind of Sophora alopecuroide oral solution according to claim 1, which is characterized in that addition dehydrated alcohol described in step 3
Amount is that the mass concentration of ethyl alcohol is 40%-60% into solution；The temperature being cooled to is 4 DEG C；The separation of solid and liquid was
Filter separation, the filter cloth filtered using 500 mesh.
5. a kind of Sophora alopecuroide oral solution according to claim 1, which is characterized in that adjustment liquor strength described in step 4 is extremely
Every 1mL medical fluid is equivalent to Sophora alopecuroide raw medicinal herbs 1g；It is described filling using Brown Glass Brown glass bottles and jars only；The temperature of the sterilizing is 121 DEG C, is gone out
The time of bacterium is 15min.
6. a kind of preparation method of Sophora alopecuroide oral solution, which is characterized in that specifically includes the following steps:
Step 1: frying: by Sophora alopecuroide, carrying out frying processing；
Step 2: extracting: the good Sophora alopecuroide of frying described in step 1 being crushed, after sieving, extraction 2-3 times is added water to cook, merges
Extracting solution after extracting solution is cooled to 40 DEG C or less, stands, is separated by solid-liquid separation, then supernatant is concentrated, obtains concentrate；
Step 3: alcohol precipitation: dehydrated alcohol is added into concentrate obtained by step 2, is cooled to 0-10 DEG C of standing, after separation of solid and liquid,
Supernatant removes ethyl alcohol, then adjusts pH value to 4.5-6.0 to get medical fluid；
Step 4: adjusting molten, filling and sterilizing: it is filling after the concentration of three gained medical fluid of set-up procedure, it sterilizes to get oral solution.
7. Sophora alopecuroide oral solution of any of claims 1-5 preparation treatment chick mycotic ingluvitis, proventriculitis,
Application in the drug of ventriculitis.
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