CN102526219B - Extract for Relinqing Granule, as well as preparation method and application of same - Google Patents
Extract for Relinqing Granule, as well as preparation method and application of same Download PDFInfo
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- CN102526219B CN102526219B CN 201210000894 CN201210000894A CN102526219B CN 102526219 B CN102526219 B CN 102526219B CN 201210000894 CN201210000894 CN 201210000894 CN 201210000894 A CN201210000894 A CN 201210000894A CN 102526219 B CN102526219 B CN 102526219B
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Abstract
The invention relates to an extract for Relinqing Granule as well as a preparation method and an application of the extract, and particularly relates to an extract of Polygonum capitatum which serves as a raw material of Relinqing Granule, as well as the preparation method and the application of the extract. The Polygonum capitatum extract is substantially prepared by the following steps: (1), boiling fresh or dried aerial parts of Polygonum capitatum in water, filtering, and concentrating and drying the filtrate; and (2), extracting the dried product obtained in the step (1) with 75% to 85% ethanol, filtering, and concentrating and drying the filtrate, to obtain the Polygonum capitatum extract. The Polygonum capitatum extract has the beneficial effects shown as the description.
Description
Technical field
The invention belongs to the field of Chinese medicines, be used to prepare the clear particulate raw material of Chinese medicine pyretic stranguria the extract that is the medical material Herba Polygoni Capitati.Particularly, the present invention relates to clear granule extract of pyretic stranguria and its production and use.More specifically, the present invention relates to be used to prepare the clear particulate raw material of pyretic stranguria is Herba Polygoni Capitati extract and its production and use.
Background technology
Herba Polygoni Capitati (Polygonum capitatum Buch-Ham ex D.Don) is a Polygonaceae Polygonum herbaceos perennial.Effect with clearing away heat-damp and promoting diuresis, inducing diuresis for treating stranguria syndrome, treating urinary system infection clinically has significant effect.Enter the national essential drugs catalogue in 2002 with its Chinese patent medicine preparation " the clear granule of pyretic stranguria " that is raw material is made, entered national basic medical insurance catalogue in 2004.
Herba Polygoni Capitati is the medicine commonly used of minority area, is mainly used in diseases such as pyelonephritis, urinary tract infection, inducing diuresis for treating stranguria syndrome.Relevant pharmaceutical research is rare, and several pieces of reports such as Ren Guangyou are only arranged at present.Ren Guangyou adopts rat bacterial pyelonephritis model to experimentize, and WBC in the Herba Polygoni Capitati water extract group rat urine and BLD and the apparent in view minimizing of matched group as a result shows that the Herba Polygoni Capitati water extract has certain antiinflammatory action to pyelonephritis.Ren Guangyou etc. observe dead mouse situation in 5 days with mouse peritoneal injection Escherichia coli bacteria liquid, and the matched group mortality rate is 100% as a result, and Herba Polygoni Capitati group mortality rate is respectively 20% and 50%, show that the Herba Polygoni Capitati water extract can resist the infection that escherichia coli cause.Ren Guangyou etc. give rabbit with Herba Polygoni Capitati water extract gastric infusion, the result, Herba Polygoni Capitati water extract group and matched group relatively, body temperature there was no significant difference, but can reduce the heating body temperature of the rabbit that the intravenous injection TAB causes.Ren Guangyou etc. are with Herba Polygoni Capitati water extract respectively gastric infusion rabbit, rat, with blank group and furosemide matched group urine amount relatively.The result shows that the Herba Polygoni Capitati water extract does not have obvious diuresis to rabbit and rat.People such as Xu Yingchun adopt agar dilution to detect the external bacteriostatic activity of Herba Polygoni Capitati to 10 strain Neisseria gonorrhoeae (gonococcus), and Herba Polygoni Capitati has bacteriostatic activity to gonococcus as a result.It is 8～32g/L to the gonococcal minimal inhibitory concentration scope of 10 strains, and meansigma methods is 11.2g/L.
Chinese patent application prospectus CN1054899A discloses a kind of miganling instant herbal medicine, syrup production technology in (Chinese patent application numbers 90107810.7, open day on October 2nd, 1991).This production technology is to be that the raw material water decocted 30-60 minute with four seasons grass, extracting liquid filtering concentrates, its supernatant concentrating under reduced pressure is become cream, reuse 60-70% ethanol extraction, with the ethanol extract vacuum drying, Herba Polygoni Capitati extractum, further be mixed with electuary or syrup, it is believed that this has detoxifcation, dissipating blood stasis, diuresis, treating stranguria effect.
Recorded the Chinese patent medicine preparation of " the clear granule of pyretic stranguria " by name in the Ministry of Health of the People's Republic of China " drug standard-Chinese traditional patent formulation preparation " (the 17) that Ministry of Health of the People's Republic of China's committee of pharmacopeia was compiled in 1998, it is by decocting with water Herba Polygoni Capitati twice concentrated the making of after-filtration.
CN 1481832A (Chinese patent application number 02129686.3, open day on March 17th, 2004) and CN 1483466A (Chinese patent application number 03146381.9, open day on March 24th, 2004) Herba Polygoni Capitati extract is disclosed, it is prepared by following steps basically: a. is by the aquatic foods product of Herba Polygoni Capitati herb or the dry product water decocts at twice or divide reflux, extract, two to three times with alcohol-water mixture, each 1-2 hour, merge decoction liquor, relative density during filtering and concentrating to 20 ℃ is 1.2, and spray drying or drying under reduced pressure obtain; Perhaps, b. carries medicinal residues by Herba Polygoni Capitati herb and water thereof and obtains through carbon dioxide supercritical fluid extraction.It is believed that this extract can be used for antibiotic, antiinflammatory, analgesia, diuresis, treatment urinary system calculus, treatment pyelonephritis and prostatitis.
Although many reports about the preparation Herba Polygoni Capitati extract are arranged at present, yet those skilled in the art expect still to obtain more efficiently product from Herba Polygoni Capitati.
Summary of the invention
The present invention seeks to expectation and obtain more efficiently product from Herba Polygoni Capitati.The inventor finds, use and earlier Herba Polygoni Capitati is carried the concentrate drying product that obtain through water, and then with these concentrate drying product of certain density ethanol extraction, the extract that obtains has the effect of desirable aspect biological activity.The present invention is based on this discovery and be accomplished.
For this reason, first aspect present invention provides a kind of Herba Polygoni Capitati extract, and it is prepared by the method that may further comprise the steps basically:
(1) bright product of Herba Polygoni Capitati aerial parts or dry product are decocted with water, filter, make filtrate concentrating, dry;
(2) with the dry thing of step (1) gained with 75～85% ethanol extractions, filter, make filtrate concentrated, dry, promptly.
Herba Polygoni Capitati extract according to first aspect present invention, in the wherein said step (1), 5-15 with Herba Polygoni Capitati dry product or bright product doubly measures water extraction 1-2 time of (preferred 6-13 doubly measures, and more preferably 6-10 doubly measures), each 1-4 hour (preferably 1-3 hour or preferably 1-2 hour).
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (1), relative density was 1.1～1.3 when filtrate was concentrated into 20 ℃.
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (1), filtrate concentrates the mode drying of afterwards spray-dried or drying under reduced pressure.
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (2), used ethanol is 80～85% ethanol, more preferably 81～84% ethanol, for example about 82% ethanol.
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (2), the dry thing of step (1) gained is ground into the fine powder of 60-120 order (preferred 80-120 order, for example about 100 orders), and then carries out ethanol extraction.
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (2), use alcoholic solution under the condition of the condition of reflow treatment or ultrasonic Treatment, to extract.In one embodiment, extraction time can be 0.5～5 hour (for example 1-4 hour, for example 1-3 hour).
Second aspect present invention provides the method for preparing Herba Polygoni Capitati extract, and it consists essentially of following steps:
(1) bright product of Herba Polygoni Capitati aerial parts or dry product are decocted with water, filter, make filtrate concentrating, dry;
(2) with the dry thing of step (1) gained with 75～85% ethanol extractions, filter, make filtrate concentrated, dry, promptly.
According to the method for second aspect present invention, in the wherein said step (1), doubly measure water extraction 1-2 time of (preferred 8-13 doubly measures, and more preferably 10-13 doubly measures), each 1-4 hour (preferably 1-3 hour or preferably 1-2 hour) with the 5-15 of Herba Polygoni Capitati dry product or bright product.
According to the method for second aspect present invention, in the wherein said step (1), relative density was 1.1～1.3 when filtrate was concentrated into 20 ℃.
According to the method for second aspect present invention, in the wherein said step (1), filtrate concentrates the mode drying of afterwards spray-dried or drying under reduced pressure.
According to the method for second aspect present invention, in the wherein said step (2), used ethanol is 80～85% ethanol, more preferably 81～84% ethanol, for example about 82% ethanol.
According to the method for second aspect present invention, in the wherein said step (2), the dry thing of step (1) gained is ground into the fine powder of 60-120 order (preferred 80-120 order, for example about 100 orders), and then carries out ethanol extraction.
According to the method for second aspect present invention, in the wherein said step (2), use alcoholic solution under the condition of the condition of reflow treatment or ultrasonic Treatment, to extract.In one embodiment, extraction time can be 0.5～5 hour (for example 1-4 hour, for example 1-3 hour).
Third aspect present invention provides the described Herba Polygoni Capitati extract of first aspect present invention in the medication of preparation clearing away heat-damp and promoting diuresis, the inducing diuresis for treating stranguria syndrome medication, urinary system infection or calculus medication, the pyelonephritis medication, detoxifcation, dissipating blood stasis medication, antibiotic, antiinflammatory, analgesia medication, the purposes in the medicine of prostatitis medication.
Fourth aspect present invention provides a kind of pharmaceutical composition, wherein comprises described Herba Polygoni Capitati extract of the arbitrary embodiment of first aspect present invention and optional pharmaceutically acceptable carrier.
According to the pharmaceutical composition of fourth aspect present invention, it is the dosage form of oral or drug administration by injection.In one embodiment, described pharmaceutical composition is the form of tablet, capsule, granule, pill, oral solutions, injection (liquid drugs injection and/or powder pin) etc.
Arbitrary technical characterictic that arbitrary embodiment had of either side of the present invention or this either side is suitable for arbitrary embodiment of other arbitrary embodiment or other either side equally, as long as they can be not conflicting, certainly at where applicable each other, necessary words can be done suitably to modify to individual features.Be further described with characteristics to various aspects of the present invention below.
All documents that the present invention quoted from, their full content is incorporated this paper by reference into, and if the expressed implication of these documents and the present invention when inconsistent, be as the criterion with statement of the present invention.In addition, various terms and phrase that the present invention uses have the general sense of well known to a person skilled in the art, nonetheless, the present invention still wishes at this more detailed description and interpretation to be made in these terms and phrase, term of mentioning and phrase are as the criterion with the implication that the present invention was explained if any inconsistent with known implication.
In the present invention, the inventive method obtains " Herba Polygoni Capitati extract ", and this term also can be described as " Herba Polygoni Capitati active component " of the present invention or " Herba Polygoni Capitati effective site " of the present invention.
In the methods of the invention, obtain water extract through step (1), the operating condition of this step need not be done special restriction, and reason is that the inventor finds that adopting the extraction solvent of unique concentration in step (2) is ethanol water, the effect of the pharmacodynamics raising of desirable occurs.
The object of the invention is to overcome the deficiencies in the prior art, and a kind of Herba Polygoni Capitati effective content of anti inflammation (effective site) and application of high-efficiency low-toxicity is provided.
Drug action focuses on to Herba Polygoni Capitati in invention, understand fully that its effect characteristics screen the drug action of the different extract parts of Herba Polygoni Capitati simultaneously, adopt the sample of the different extract parts of Herba Polygoni Capitati to carry out the screening of Herba Polygoni Capitati active component (effective site) with the drug action characteristics by antiinflammatory, analgesia, antibiotic pharmacological evaluation.
In one embodiment, invention is achieved through the following technical solutions above-mentioned purpose:
Invention provides a kind of Herba Polygoni Capitati active component (effective site), is to be obtained by following steps:
(1) get bright product of Herba Polygoni Capitati aerial parts or dry product, decoct with water 2～4 hours, filter, relative density was 1.1～1.3 when filtrate was concentrated into 20 ℃, and concentrated solution spray drying or drying under reduced pressure become dry thing;
(2) dry thing is ground into 100 order fine powders, and 75～85% ethanol extractions of 8 times of quality of fine powder reuse 1 hour filter, and filtrate is drying to obtain.Ethanol in the filtrate can reclaim reuse.
In one embodiment, the addition of the described water of step (1) is Herba Polygoni Capitati dry product or bright product 10-13 a times; Decoct with water at twice and carry out, each 1～2 hour.
In one embodiment, the described ethanol extraction of step (2) carries out at twice, and each 0.5 hour, described being extracted as stirred extraction or ultrasonic extraction.
In one embodiment, in the alcoholic acid leaching process of step (2), alcoholic acid mass fraction is an important factor, is related to the medicinal effects of the effective site of being extracted.Concentration of alcohol is low, and Herba Polygoni Capitati medicated powder adheres to easily, causes and extracts not exclusively, the commercial production difficulty.The high polar component of concentration of alcohol extracts and reduces, and influences drug effect.The highly preferred concentration of alcohol of the present invention is 80～83%, is more preferably 82%.
A preferred embodiment of the present invention below is provided, the Herba Polygoni Capitati active component is to be obtained by following method: decocted with 13 times of water (W/W) by the Herba Polygoni Capitati dry product, can be divided into secondary decocts, each 1～2 hour, filter, merging filtrate, relative density was 1.1～1.3 when filtrate was concentrated into 20 ℃, concentrated solution spray drying or drying under reduced pressure, drying under reduced pressure thing are ground into 100 order fine powders.8 times of (W/W) 82% ethanol extractions of dry fine powder reuse divide second extraction (ultrasonic extraction), and each 0.5 hour, filter, merging filtrate reclaims ethanol, and residue dried is promptly.
Herba Polygoni Capitati active component provided by the present invention (effective site), xylol causes the influence experiment of mice ear, press 5g Herba Polygoni Capitati dry product extract obtained/kg body weight gastric infusion, continuous 5 days, test behind the last administration 60min, significant antiinflammatory action is arranged, and the highest inflammation suppression ratio can reach 46.08%; Cause the pain experiment for the mice hot plate, can prolong the mice hot plate and add foot reaction latent time (comparing P＜0.05 with matched group), the highest time-delay rate can reach 16.57%.
This Herba Polygoni Capitati active component can become various common dosage forms and slow releasing agent, controlled release agent, targeting preparation etc. with acceptable adjuvant combined preparation in the medicine manufacturing.
Water is carried although the Herba Polygoni Capitati medicine adopts, the technology of alcohol extraction, decocting in water alcohol extraction is comparatively known way, but water breakthrough (boiling) promotion is not dry, pulverizes, the technology of specific concentrations ethanol extraction again, compared with prior art, the present invention has the different of following beneficial effect and character.
The present invention extracts with decocting in water earlier, and the method that obtains active ingredient and then concentrate with polar solvent (ethanol of specific concentrations) can make antiinflammatory action than the obvious enhancing of water extract.The extract of extract of the present invention and extraction process by water relatively can improve its antiphlogistic effects very significantly, and its experimental result is as shown in the table:
| Group | Dosage (g/kg) | n | Swelling degree (mg) | Suppression ratio (%) |
| Matched group | - | 10 | 6.8±3.01 | |
| The Herba Polygoni Capitati water extract | 2.5 | 10 | 5.3±1.35 | 22.06 |
| The Herba Polygoni Capitati water extract | 5 | 10 | 4.8±1.55 | 29.41 |
| Extract of the present invention | 2.5 | 10 | 4.4±1.26 ＊ | 35.29 |
| Extract of the present invention | 5 | 9 | 3.67±1.00 ＊＊ | 46.08 |
Annotate: compare with matched group:
*P＜0.05
*P＜0.01
In the last table, the extracting method that " extract of the present invention " adopts is: the Herba Polygoni Capitati dry product is divided into secondary with 13 times of water (W/W) decocts, each 1.5 hours, filter, merging filtrate, relative density was 1.1 when filtrate was concentrated into 20 ℃, and the concentrated solution spray drying gets the above fine powder of 100 orders (this fine powder is the reagent of going up table " Herba Polygoni Capitati water extract ").8 times of (W/W) 82% ethanol extractions of dry fine powder reuse divide second extraction (ultrasonic extraction), and each 0.5 hour, filter, merging filtrate reclaims ethanol, and residue dried promptly gets (as the reagent of " extract of the present invention " in the last table).
In the present invention, during the dosage representing to test with g/kg, as do not have other explanation, be meant that every kg the weight of animals gives the corresponding reagent weight that (extract for example of the present invention) amounts to into the Herba Polygoni Capitati dry product.
The specific embodiment
In order more to be expressly understood technology contents of the present invention, describe in detail especially exemplified by following examples, but embodiments of the present invention are not limited thereto.
A, extract preparation example part
Preparation example 1: Herba Polygoni Capitati water extract
Get Herba Polygoni Capitati aerial parts dry product 10Kg, wash, add 10 times of water gagings (W/W), soaked 1 hour, heated and boiled 1 hour, filter, filtering residue adds 6 times of water gagings again and boiled 1 hour, filters, merging filtrate, relative density reached 1.3 when filtrate was concentrated into 20 ℃, and the concentrated solution spray drying gets dry fine powder 1.03Kg.
Preparation example 2: the preparation of extract of the present invention
Get dry fine powder (the about 100 orders) 0.5Kg of preparation example 1 method gained, divide twice ultrasonic to extract with 8 times of (W/W) 82% ethanol, each 0.5 hour, filter, merging filtrate reclaims ethanol, the residue drying under reduced pressure, get dry thing 0.047Kg, this extract is used for hereinafter testing the reagent of routine part test.
Preparation example 2a: the preparation of extract of the present invention
In addition,, use 6 times of (W/W) 82% ethanol, 10 times of (W/W) 82% ethanol to extract respectively and supersound process 2 times each 5 hours, extract yield and preparation example 2 basic identical with reference to the method for above preparation example 2; Equally hereinafter test routine part test with extract obtained again, the result shows the extract obtained biologic activity basic identical (differing less than 10%) of 8 times of (W/W) 82% ethanol extractions of usefulness with preparation example 2, show that under the condition of same concentrations extracting solution extraction conditions such as extracting solution consumption and alcohol processing time do not have influence to the activity of extract.
Preparation example 3: the preparation of extract of the present invention
Get dry fine powder (the about 80 orders) 0.5Kg of preparation example 1 method gained, with 8 times (W/W) 75% alcohol reflux 1 time, each 1 hour, filter, merging filtrate reclaims ethanol, and the residue drying under reduced pressure must dry thing 0.068Kg.
Preparation example 4: the preparation of extract of the present invention
Get dry fine powder (the about 120 orders) 0.5Kg of preparation example 1 method gained, divide twice ultrasonic to extract with 8 times of (W/W) 85% ethanol, each 3 hours, filter, merging filtrate reclaims ethanol, and the residue drying under reduced pressure gets dry thing 0.052Kg.
Preparation example 5: the preparation of extract of the present invention
Get the bright product 20Kg of Herba Polygoni Capitati aerial parts, wash, add 7 times of water gagings (W/W), soaked 1 hour, heated and boiled 1 hour, filter, filtering residue adds 5 times of water gagings again and boiled 1 hour, filters, merging filtrate, relative density reached 1.1 when filtrate was concentrated into 20 ℃, and the concentrated solution spray drying gets dry fine powder 0.63Kg.
Get dry fine powder (the about 100 orders) 0.5Kg of above preparation, carry out alcohol extraction with reference to the method for preparation example 4 then, difference is that used ethanol is 80% ethanol, gets dry thing 0.055Kg.
Reference examples 1: the preparation of reference extract
With reference to the method for preparation example 2, difference is that used ethanol is 70%, gets dry thing 0.075Kg.
Reference examples 2: the preparation of reference extract
With reference to the method for preparation example 2, difference is that used ethanol is 60%, gets dry thing 0.083Kg.
Reference examples 3: the preparation of reference extract
With reference to the method for preparation example 2, difference is that used ethanol is 90%, gets dry thing 0.033Kg.
Reference examples 4: the preparation of reference extract
With reference to the method for preparation example 5, difference is that the used ethanol of alcohol extraction step is 72%, gets dry thing 0.081Kg.
Reference examples 5: the preparation of reference extract
With reference to the method for preparation example 5, difference is that the used ethanol of alcohol extraction step is 88%, gets dry thing 0.036Kg.
B, pharmacodynamics test example part
Test example 1: the experiment of mice caused by dimethylbenzene xylene ear swelling, the antiphlogistic effects of investigation Herba Polygoni Capitati extract
Animal: SPF level Kunming mouse, body weight 18～22g, male and female dual-purpose.
Reagent, dosage and medication: as reagent, dosage is that to give the weight that corresponding reagent amounts to into the Herba Polygoni Capitati dry product be 5g to every kg the weight of animals with the various preparation example samples of " A, extract preparation example part " gained above and reference examples sample.Each reagent is faced with preceding and is suspended to the concentration that is equivalent to every each gastric infusion 0.4ml of animal with normal saline, is provided with simultaneously to irritate the matched group of stomach with the volume normal saline.
Test method: mice male and female dual-purpose, random packet, 10 animals of each reagent group; Successive administration 5 days behind the last administration 60min, only is coated with auris dextra with dimethylbenzene 0.1ml/ and causes inflammation, puts to death mice behind the 30min, two auricles about getting with the card punch of diameter 6mm.Use scales/electronic balance weighing immediately, as swelling degree (mg), calculate the suppression ratio (%) of each reagent group with following formula with two auricle weight differences:
Wherein swelling degree (mg)=auris dextra sheet weight-left auricle is heavy
The result carries out statistical analysis.Experimental result sees Table 1.
Suppression ratio is carried out classification, and suppression ratio is lower than 10% and is the A level, and suppression ratio 10～20% is the AA level, and suppression ratio 20～30% is the AAA level, and suppression ratio 30～40% is the AAAA level, and suppression ratio 40～50% is the AAAAA level, and suppression ratio is the AAAAAA level greater than 50%.
Table 1: different Herba Polygoni Capitati xylol causes the influence of mice ear
| Reagent | The suppression ratio classification |
| Preparation example 1 | AAA ＊ |
| Preparation example 2 | AAAA ＊＊ |
| Preparation example 3 | AAAAA ＊＊ |
| Preparation example 4 | AAAAA ＊＊ |
| Preparation example 5 | AAAAAA ＊＊ |
| Reference examples 1 | AA |
| Reference examples 2 | AAA ＊ |
| Reference examples 3 | A |
Annotate: swelling degree (mg), compare with the solvent control animals:
*P＜0.05,
*P＜0.01.In addition, the result of reference examples 4 and reference examples 5 reagents is respectively AA and A.
Test example 2: the heat-resisting hot plate reaction experiment of mice, the analgesic effect of investigation Herba Polygoni Capitati extract
Animal, reagent, dosage and medication are identical with test example 1.
Test method: mice male and female dual-purpose, random packet, 10 animals of each reagent group; Successive administration 5 days behind the last administration 60min, is put into intelligent hot-plate instrument with each group mice, and the observed and recorded mice is added foot reaction latent time, and each is organized mice measurement 2 times (each about 15min at interval) and gets its meansigma methods.Experimental result sees Table 2.
Carry out classification to adding foot reaction latent time, latent time is lower than 6 seconds and is the A level, and latent time 6～7 seconds was the AA level, and latent time 7～8 seconds was the AAA level, and latent time 8～9 seconds was the AAAA level, and latent time was the AAAAA level greater than 9 seconds.
Table 2: different Herba Polygoni Capitati is to the influence of the heat-resisting hot plate reaction of mice
| Reagent | Add the classification of foot reaction latent time |
| Preparation example 1 | AA |
| Preparation example 2 | AAAA ＊＊ |
| Preparation example 3 | AAA ＊ |
| Preparation example 4 | AAAA ＊＊ |
| Preparation example 5 | AAAA ＊＊ |
| Reference examples 1 | AA |
| Reference examples 2 | A |
| Reference examples 3 | A |
| The solvent matched group | A |
Annotate: add foot reaction latent time (second), compare with the solvent control animals:
*P＜0.05
*P＜0.01.In addition, reference examples 4 and reference examples 5 reagent latent time classification results are the A level.
Embodiment 3: the antibacterial experiment of different Herba Polygoni Capitati extract
Adopt conventional antibacterial tests method, use the MH broth bouillon that bacillus pyocyaneus, escherichia coli M421C1ST, dysentery bacterium, staphylococcus aureus, Hemolytic streptococcus are tested, as reagent, test their sensitivity with the various preparation example samples of " A, extract preparation example part " gained above and reference examples sample to these cells.
The result shows that preparation example 1 sample is to bacillus pyocyaneus, escherichia coli sensitivity, MIC＞20mg/ml; Each sample of reference examples 1-3 is insensitive to bacillus pyocyaneus, escherichia coli, dysentery bacterium, staphylococcus aureus, to streptococcic MIC＞10mg/ml; Each sample of preparation example 2-5 to the MIC of five kinds of antibacterials between 1-4mg/ml, to the MIC=2.5mg/ml of individual cells.
Claims (16)
1.
A kind of Herba Polygoni Capitati extract, it is prepared by the method that may further comprise the steps:
(1) the bright product of Herba Polygoni Capitati aerial parts or dry product are added water boiling and extraction 1-2 time that 5-15 doubly measures, 1-4 hour at every turn, filtration, when making filtrate be concentrated into 20 ℃ relative density be 1.1 ~ 1.3, drying;
(2) with the dry thing of step (1) gained with 75 ~ 85% ethanol extractions, filter, make filtrate concentrated, dry, promptly.
2.
According to the Herba Polygoni Capitati extract of claim 1, in the wherein said step (1), filtrate concentrates the mode drying of afterwards spray-dried or drying under reduced pressure.
3.
According to the Herba Polygoni Capitati extract of claim 1, in the wherein said step (2), used ethanol is 80 ~ 85% ethanol.
4.
According to the Herba Polygoni Capitati extract of claim 1, in the wherein said step (2), used ethanol is 81 ~ 84% ethanol.
5.
According to the Herba Polygoni Capitati extract of claim 1, in the wherein said step (2), used ethanol is 82% ethanol.
6.
According to the Herba Polygoni Capitati extract of claim 1, in the wherein said step (2), the dry thing of step (1) gained is ground into 60-120 purpose fine powder, and then carries out ethanol extraction.
7.
According to the Herba Polygoni Capitati extract of claim 1, in the wherein said step (2), use alcoholic solution under the condition of the condition of reflow treatment or ultrasonic Treatment, to extract.
8.
Prepare the method for Herba Polygoni Capitati extract, it may further comprise the steps:
(1) the bright product of Herba Polygoni Capitati aerial parts or dry product are added water boiling and extraction 1-2 time that 5-15 doubly measures, 1-4 hour at every turn, filtration, when making filtrate be concentrated into 20 ℃ relative density be 1.1 ~ 1.3, drying;
(2) with the dry thing of step (1) gained with 75 ~ 85% ethanol extractions, filter, make filtrate concentrated, dry, promptly.
9.
Method according to Claim 8, in the wherein said step (1), filtrate concentrates the mode drying of afterwards spray-dried or drying under reduced pressure.
10.
Method according to Claim 8, in the wherein said step (2), used ethanol is 80 ~ 85% ethanol.
11.
Method according to Claim 8, in the wherein said step (2), used ethanol is 81 ~ 84% ethanol.
12.
Method according to Claim 8, in the wherein said step (2), used ethanol is 82% ethanol.
13.
The purposes of each described Herba Polygoni Capitati extract of claim 1-7 in antibiotic, the antiinflammatory of preparation, analgesic medicine.
14.
A kind of pharmaceutical composition wherein comprises each described Herba Polygoni Capitati extract of claim 1-7 and optional pharmaceutically acceptable carrier.
15.
The pharmaceutical composition of claim 14, it is the dosage form of oral or drug administration by injection.
16.
The pharmaceutical composition of claim 14, it is the form of tablet, capsule, granule, pill, oral solutions, injection.
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| CN102716194B (en) * | 2012-02-13 | 2014-08-06 | 谢雁鸣 | Polygonum capitatum composition for preventing or treating whelk |
| CN103977086A (en) * | 2014-04-25 | 2014-08-13 | 贵州三泓药业股份有限公司 | Preparation method of polygonum capitatum instant powder |
| CN105434555A (en) * | 2015-12-30 | 2016-03-30 | 贵州弘康药业有限公司 | Production method of Relinqing preparation |
| CN115607691B (en) * | 2022-11-01 | 2024-04-09 | 贵州威门药业股份有限公司 | Relinqing granule with excellent antipyretic effect |
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| CN1054899A (en) * | 1990-09-14 | 1991-10-02 | 贵阳生物化学制药厂 | Miganling instant herbal medicine, syrup production technology |
| CN1481832A (en) * | 2002-09-12 | 2004-03-17 | 贵州威门药业股份有限公司 | Polygonum capilalum extract , iIts preparation method and use |
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| CN101664446A (en) * | 2009-09-28 | 2010-03-10 | 贵州威门药业股份有限公司 | Relinging extractum and preparation method and application |
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| CN101940625A (en) * | 2010-09-07 | 2011-01-12 | 中国中医科学院中药研究所 | Preparation method and use of extract |
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