CN101953866A - Preparation method of white-backed pseudo-ginseng total flavonoid as well as application - Google Patents
Preparation method of white-backed pseudo-ginseng total flavonoid as well as application Download PDFInfo
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Abstract
The invention relates to a preparation method of white-backed pseudo-ginseng total flavonoid. The method comprises the following steps of: taking, heating and adding white-backed pseudo-ginseng, adding 40-95% of ethanol for reflux extraction; concentrating an extract and adding a macroporous resin column; carrying out the gradient elution of aqueous ethanol and collecting the elution part of 35-55% of ethanol; and recycling solvent to obtain total flavonoid which can be mixed with pharmaceutical accessories to prepare medicaments with the hyperglycemic action. The method has good reproducibility, reduced impurities and high total flavonoid content so as to ensure stable curative effect and stimulate the industrial production. The extractive can be used for preparing medicaments or health products with the hyperglycemic action.
Description
Technical field
The present invention relates to the extraction separation method of effective ingredient in Chinese, specifically extraction separation has the method for hypoglycemic activity total flavones from the Chinese medicine Radix et Rhizoma Gynurae divaricatae, belongs to medicine separation and Extraction field.
Background technology
Radix et Rhizoma Gynurae divaricatae (Gynura divaricata (L.) DC.) is a Compositae Radix gynurae segeti platymiscium, have another name called Bai Dongfeng, white chessman grass, beautiful Folium Eriobotryae, Asiatic toddalia root-bark, thick dough cover, chicken dish, big cattle, white kind of Herba Amaranthi tricoloris, white Herba Gynurae bicoloris, furuncle pull out, and are distributed in Taiwan to south China, a southwestern band.Its root, stem, Ye Junke are used as medicine.Its root nature and flavor are sweet, cool, energy clearing away heat and cooling blood, dissipating blood stasis for subsidence of swelling; Its stem, the salty little suffering of leaf nature and flavor, cold, poisonous have heat clearing away, Shujin, hemostasis, the effect of eliminating the phlegm.In Fujian, among the people with its stem and leaf make tea drinking-water take, be used for the treatment of diabetes, effect is fine.The Radix et Rhizoma Gynurae divaricatae total flavones have preferably hypoglycemic activity (Hu Yong etc., the research of Radix et Rhizoma Gynurae divaricatae aerial parts hypoglycemic activity, the Xi'nan College of Forestry journal, 2007,27(1): 55-58; Jiang Manhua etc., Radix et Rhizoma Gynurae divaricatae polysaccharide and flavone blood sugar lowering and resisting oxygen lack, the Chinese Hospitals pharmaceutical journal, 2009,29(13): 1074-1076).
At present, rarely seen 4 pieces of the report of extraction separation total flavonoids substance from Radix et Rhizoma Gynurae divaricatae.1. Caulis et Folium Gynurae divaricatae is crushed to 12 orders, soaks 50 min, then with 40 times of amount ethanol of 60%, 60 ℃ of following supersound extraction 2 times.(Wang Jinjiang etc., the Study on extraction of Radix et Rhizoma Gynurae divaricatae total flavones.The China pharmacist, 2009,12(2), 146-149) 2. Radix et Rhizoma Gynurae divaricatae extracts three times repeatedly through 95% ethanol, and the extractum after extracting solution concentrates is suspended in the water, behind petroleum ether extraction, water is again through n-butanol extraction, and polyamide column on the n-butyl alcohol extract gets total flavones behind the ethanol gradient elution.(Hu Yong etc., the research of Radix et Rhizoma Gynurae divaricatae aerial parts hypoglycemic activity, the Xi'nan College of Forestry journal, 2007,27(1): 55-58).3. Radix et Rhizoma Gynurae divaricatae is behind 60% ethanol ultrasonic extraction, and polyamide column on the extract gets total flavones behind 70% ethanol elution.(the thick congruence of Lee, the research of polyamide purifying Radix et Rhizoma Gynurae divaricatae total flavones, Chinese pharmacist, 2010,13(2): 172-175)
Above-mentioned 3 kinds of methods extraction separation total flavonoids substance from Radix et Rhizoma Gynurae divaricatae, impurity is more in the extract, used polyamide cost height, and need to use toxic organic solvent, flavones content is not high and the rate of transform is low.
Summary of the invention
The technical problem to be solved in the present invention is that improvement extraction separation from Radix et Rhizoma Gynurae divaricatae has the method for hypoglycemic activity total flavones, make its not only favorable reproducibility, and extraction cost is low, impurity reduces, the general flavone content height, thus the stable of curative effect guaranteed, and help suitability for industrialized production.
The objective of the invention is to realize by following scheme:
Scheme 1, get Radix et Rhizoma Gynurae divaricatae, pulverize, add the 40-95%(weight ratio, down with) alcohol reflux, extracting solution concentrates the back and goes up macroporous resin column, use the ethanol water gradient elution, collects 35-55% ethanol elution part, the concentrated total flavones that obtains; Described macroporous resin is the D-101 macroporous resin.
Scheme 2, Radix et Rhizoma Gynurae divaricatae are ground into 40 order powder, use the 45-70% alcohol reflux, and extracting solution concentrates the back and goes up the D-101 macroporous resin column, uses the ethanol water gradient elution, collect 40-50% alcohol eluting part, concentrate and obtain total flavones.
Scheme 3, Radix et Rhizoma Gynurae divaricatae are ground into 40 order powder, adopt the 50-80% alcoholic solution of 6-10 times of medical material weight (W/V), reflux, extract, under 80-95 ℃ of condition, extracting solution concentrates the back and goes up the D-101 macroporous resin column, use the ethanol water gradient elution, collect 40-50% ethanol elution part, concentrate and obtain total flavones.
Scheme 4, Radix et Rhizoma Gynurae divaricatae are ground into 40 order powder, adopt the 50-55% alcoholic solution of 7-9 times of medical material weight (W/V), reflux, extract, is 2 times under 85-90 ℃ of condition, extracting solution concentrates the back and goes up the D-101 macroporous resin column, increase progressively eluting with the ethanol water gradient, collect 40-50% ethanol elution part, concentrate and obtain total flavones.
Scheme 5, Radix et Rhizoma Gynurae divaricatae are ground into 40 order powder, adopt 50% alcoholic solution of 8 times of medical material weight (W/V), reflux, extract, is 2 times under 85 ℃ of conditions, extracting solution concentrates the back and goes up the D-101 macroporous resin column, use 25%, 45%, 70%, 95% alcoholic solution gradient elution successively, collect 45% ethanol elution, concentrate and obtain total flavones.
The Radix et Rhizoma Gynurae divaricatae total flavones of above-mentioned preparation can mix with suitable carrier pharmaceutically, and preparation has the medicine of hypoglycemic activity.
In order to realize above purpose, the applicant has carried out useful test from following each side:
1, extracting mode is to the influence of extraction efficiency
(1) medical material is selected
Select the leaf of Compositae Radix gynurae segeti platymiscium Radix et Rhizoma Gynurae divaricatae (Gynura divaricata (L.) DC.) for use, the properties and characteristics of medical material, powder characteristics, physicochemical property should meet the content under Fujian Province's Chinese crude drug standard (version in 2006) " white chessman grass " item.
(2) extract solvent
The contained flavone of Radix et Rhizoma Gynurae divaricatae mostly is the bigger chemical compound of flavonoid glycoside polarity, and methanol or ethanol are good extraction solvents, but methanol toxicity is bigger, so the present invention's extraction solvent of alcoholic solution as total flavones.
(3) extracting mode
Get respectively and be ground into the about 20g of 40 purpose medicinal powders, the accurate title, decide, and extracts by following method with 8 times of amount 50% ethanol:
Merceration: measured solvent cold soak 12 hours, and extracted 2 times for 8 times.
Percolation: 16 times of amount solvent percolation.
Hot reflux: 85 ℃ of hot refluxs of 8 times of amount solvents, extract 2 times.
Merge various extracting solution respectively, measure total flavones amount in the extract respectively by ultraviolet spectrophotometry, the result is as shown in table 1.
Table 1 extracting mode is to the table that influences of extraction efficiency
As shown in Table 1, the extraction efficiency that heat is carried is the highest, and percolation takes second place, and merceration is relatively poor, and three's significant difference.
2, the optimization of extraction process
In order to determine the influence to extraction efficiency of concentration of alcohol, consumption, temperature and extraction time, we have carried out positive quadraturing design test research, are index with the content of total flavone, and extraction process is optimized.Extract with heat reflow method, extracting solution is pressed general flavone content in the determined by ultraviolet spectrophotometry extract after concentrating.
The extraction process orthogonal experiment relates to solvent strength, temperature, solvent load, extraction time etc., measures total flavones amount, extractum amount and total flavones yield respectively.
Take all factors into consideration total flavones and extract total amount, content and yield, be attached to the cost of suitability for industrialized production, the difficulty or ease of post processing simultaneously, and the factors such as stable and repeatability of technology, the present invention selects the 40-95% ethanol by 6-10 times of volume of medical material weight for use, carries out reflux, extract, under 80-95 ℃ of condition.The extraction separation condition is preferably: with 45-70% ethanol of 7-9 times of volume of medical material weight, reflux, extract.Preferable condition also has: with the 50-60% ethanol of 7-9 times of volume of medical material weight, reflux, extract, is 2 times under 80-90 ℃ of condition.Better condition is: with 50% ethanol of 8 times of volumes of medical material weight, reflux, extract, is 2 times under 85 ℃ of conditions.
3, the separation of Radix et Rhizoma Gynurae divaricatae total flavones, purification, concentrated and exsiccant optimised process
(1) isolation and purification technology
The Radix et Rhizoma Gynurae divaricatae extract is used 25%, 45%, 70%, 95% ethanol elution successively through the segmentation of D-101 macroporous resin, collects 45% ethanol elution part, and the concentrating under reduced pressure after drying promptly gets the total flavones part.
(2) concentrated and drying process
45% ethanol elution is partly filtered, decompression and solvent recovery, vacuum drying promptly gets total flavone part, and wherein content of total flavone can reach 50-60%, and the rate of transform of total flavones can reach 60～85% in the medical material.
The used medicinal material processing method of technology of the present invention is relatively to draw through experiment, extracts process orthogonal Design Research such as solvent species and concentration, extracting method, pulverizing medicinal materials order number and forms, and has guaranteed resulting Radix et Rhizoma Gynurae divaricatae total flavones yield height; The eluting segmentation of macroporous resin, the small concentration gradient experiment through strict has guaranteed that the content of main flavone (rutin, nicotiflorin, Herba Astragali Melilotoidis (Herba Astragali Sinici) glycosides etc.) in the resulting segmentation position reaches 50 ~ 60%, the rate of transform of total flavones is 60 ~ 85% in the medical material.
The specific embodiment
Below be several concrete case study on implementation of the present invention, further describe the present invention, but the present invention be not limited only to this.
Embodiment 1
Get Radix et Rhizoma Gynurae divaricatae medical material 20kg, be ground into 40 order powder, adopt the 45%(weight ratio of 6 times of volumes of medical material weight, down with) 75 ℃ of hot refluxs of ethanol extract each 1 hour three times.Extracting solution merges, decompression and solvent recovery, and the gained concentrated solution is the alcoholic solution that gradient increases with D101 macroporous resin segmentation, eluant.Collect 35-55% ethanol section eluent, decompression and solvent recovery, gained extractum promptly get Radix et Rhizoma Gynurae divaricatae total flavones crude product 0.17kg through vacuum drying, and its general flavone content is 50%, and the rate of transform is 66%.
Embodiment 2
Get Radix et Rhizoma Gynurae divaricatae medical material 20kg, be ground into 40 order powder, adopt 85 ℃ of hot refluxs of 70% ethanol of 8 times of volumes of medical material weight to extract secondary, each 1 hour.Extracting solution merges, decompression and solvent recovery, and the gained concentrated solution is the alcoholic solution that gradient increases with D101 macroporous resin segmentation, eluant.Collect 45-50% ethanol section eluent, decompression and solvent recovery, gained extractum promptly get Radix et Rhizoma Gynurae divaricatae total flavones coarse-grain 0.16kg through vacuum drying, and its general flavone content is 55%, and the rate of transform is 70%.
Embodiment 3
Get Radix et Rhizoma Gynurae divaricatae medical material 20kg, be ground into 40 order powder, adopt the 95% alcohol 95 ℃ hot reflux of 10 times of volumes of medical material weight to extract secondary, each 1 hour.Extracting solution merges, decompression and solvent recovery, and the macroporous resin segmentation of gained concentrated solution, eluant is the alcoholic solution that gradient increases.Collect 45-55% ethanol section eluent, decompression and solvent recovery, gained extractum promptly get Radix et Rhizoma Gynurae divaricatae total flavones crude product 0.15kg through vacuum drying, and its general flavone content is 51%, and the rate of transform is 60%.
Embodiment 4
Get Radix et Rhizoma Gynurae divaricatae medical material 20kg, pulverize medical material to 40 order,, use 50% ethanol in the hot reflux mode, the solvent of 8 times of amount volumes, 85 ℃ are extracted 2 times down.Merge extractive liquid,, the weight ratio that is evaporated to concentrated solution volume and medical material is 0.8-1.0.Concentrated solution multilamellar filtered through gauze, be splined on D-101 macroporous resin top, water, 25%, 45%, 70%, 95% ethanol gradient elution is collected 45% ethanol section eluent, decompression and solvent recovery, vacuum drying gets 0.18kg Radix et Rhizoma Gynurae divaricatae total flavones crude product, and its general flavone content is 59%, and the rate of transform is 84%.
Implement 5 total flavones blood sugar reducing functions
1 experiment material
3.1.1 laboratory animal
SPF level SD rat, 60, ♂, body weight 150～180g, available from Shanghai Slac Experimental Animal Co., Ltd., the quality certification number: SOXK(Shanghai) 2008-0003.Cleaning level muroid laboratory is raised and is tested in hospital general, Foochow laboratory animal section.
2 medicines and reagent
Metformin hydrochloride tablet: SZYY Group Pharmaceutical Limited.'s product, lot number: 07010906;
The glibenclamide sheet: Tianjin Pacific Pharmaceutical Co., Ltd. produces, lot number: 070404;
The Radix et Rhizoma Gynurae divaricatae total flavones extracts purification according to the described method of this patent and gets;
Cholesterol is Shanghai chemical reagents corporation of a Chinese Medicine group product, lot number: F 20061114;
Sodium cholate is Shanghai chemical reagents corporation of a Chinese Medicine group product, lot number: F 20060428;
Streptozotocin (STZ) and citric acid/trisodium citrate: Sigma company;
Glacial acetic acid and concentrated sulphuric acid are analytical pure, available from Shanghai joint-trial chemical reagent company limited;
Liver glycogen is measured test kit: bio-engineering research institute is built up in Nanjing;
Superoxide dismutase (SOD) testing cassete and malonaldehyde (MDA) are measured test kit: build up bio-engineering research institute available from Nanjing;
Insulin (Ins) radioimmunoassay, RIA medicine box, Endothelin (ET) radioimmunoassay, RIA medicine box: available from Beijing North biotechnology research institute.
Radix et Rhizoma Gynurae divaricatae total flavones: obtain by embodiment 1.
3 experiment key instruments
Optium blood glucose/blood ketone instrument and supporting reagent paper: U.S. Abbott;
Electronic balance: FA1004, Shanghai level instrument plant product;
Low speed refrigerated centrifuge: HDC-2044, good branch company product in the Keda Innovation Co., Ltd;
Radioimmunity enumerator: GC-1200 γ, good photoelectric instrument company product in the University of Science and Technology of China,technology industry Corp;
Ultraviolet-visible spectrophotometer: UV-2501PC, day island proper Tianjin company produces;
Automatic clinical chemistry analyzer: AU-2700, Olympus company produces;
4 experimental techniques
4.1 the foundation of diabetes animal model
After SPF level male SD rat adaptability is raised a week, randomly draw 8 as the normal control group, (make by oneself by feedstuff with the high glucose and high fat feedstuff for all the other rats, consist of: 10.0% Adeps Sus domestica, 20.0% sucrose, 2.5% cholesterol, 1.0% cholate, 66.5% conventional feed) nursing 30d.Behind the fasting water 12h, with concentration is fresh preparation 2% streptozotocin of citric acid/trisodium citrate buffer (STZ) solution of 0.1mmol/L, pH4.2 ~ 4.5, rat is cut the tail blood sampling and detects blood glucose, with blood glucose with 30mg/kg dosage lumbar injection after 72 hours〉16.7 mmolL
-1For successful model is included experiment in.
4.2 experiment grouping and administration
Get 40 of diabetes model rats and be divided into 5 groups at random, every group 8, wherein 1 group is model control group, all the other 4 groups is the administration group, be metformin group (25mg/kgd), glibenclamide group (2mg/kgd), Radix et Rhizoma Gynurae divaricatae total flavones low dose group (20mg/kgd) and high dose group (80mg/kgd), face with preceding and all be mixed with suspension with 0.5% CMC-Na solution.Each is organized rat and all irritates stomach and give relative medicine, and normal control group and model control group wait 0.5% CMC-Na solution of capacity.Irritate between gastric phase the normal control group and give normal feedstuff and feed, all the other each treated animals continue to feed with the high glucose and high fat feedstuff.The adjustment dosage of regularly weighing in, 1 time/d, 30d continuously.
4.3 index detects
Experimental session is cut tail respectively at 0 d, 10 d, 20 d, 30 d and is got blood examination survey blood glucose.After administration finished, (45 mg/kg) anaesthetized with the rats by intraperitoneal injection pentobarbital sodium, heart extracting blood 7～10ml, and separation of serum ,-20 ℃ of refrigerators are preserved to be measured.Adopt AU-2700 type automatic clinical chemistry analyzer to detect blood glucose, blood lipid level, and measure SOD and MDA content in strict accordance with the test kit operation instructions, radioimmunology detects serum I ns, blood plasma ET.Cut open and get fresh liver, behind the normal saline flushing, measure liver glycogen content in strict accordance with the test kit operation instructions.
4.4 statistical analysis
Experimental data is all carried out statistical procedures with SPSS13.0 software, every index result with mean ± standard deviation (
±
s) expression, relatively adopt the t check between group, with
P＜0.05 for there being statistically-significant difference.
5 results
5.1 the Radix et Rhizoma Gynurae divaricatae total flavones is to the influence of type 2 diabetes mellitus rat model blood glucose
Behind the rats by intraperitoneal injection STZ, blood glucose significantly raises, and injects 10d, 20d, 30d model group rat blood sugar is very high, with normal group relatively, have significant difference (
p＜0.01), type 2 diabetes mellitus model modeling success is described, and continues hyperglycemia at experimental session.Respectively organize rat blood sugar behind the administration 10d and begin to descend, 20d after the administration, the Radix et Rhizoma Gynurae divaricatae total flavones is low, the high dose group rat blood sugar descends obviously, with model group relatively, have significant difference (
p＜0.05).Irritate behind the stomach 30d that the Radix et Rhizoma Gynurae divaricatae total flavones is low, high dose group rat blood sugar and model group relatively have significant differences (
p＜0.01), high dose group and metformin, glibenclamide matched group blood sugar decreasing effect quite (
p0.05).Results suggest, the Radix et Rhizoma Gynurae divaricatae total flavones has hypoglycemic activity preferably, and can demonstrate certain dose-effect, time-effect relationship.See Table 5.
Table 5: the Radix et Rhizoma Gynurae divaricatae total flavones to the influence of T2DM rat blood sugar (n=8,
±
s)
Annotate: compare with the normal control group,
a p＜0.01; Compare with model control group,
b p＜0.05,
c p＜0.01
Compare with the metformin matched group,
d p0.05; Compare with the glibenclamide matched group,
e p0.05
5.2 the Radix et Rhizoma Gynurae divaricatae total flavones is to the influence of type 2 diabetes mellitus rat model blood fat
Each treated animal was irritated stomach after 30 days, compares with the normal control group, all obviously risings of T-CHOL in the model group rat serum (TC) and triglyceride (TG) level (
p＜0.01), illustrates that tangible disorders of lipid metabolism has appearred in the type 2 diabetes mellitus rat model; Compare with model control group, the Radix et Rhizoma Gynurae divaricatae total flavones is low, the TC of high dose group rat and TG level all obviously descend (
p＜0.01,
p＜0.05).Results suggest Radix et Rhizoma Gynurae divaricatae total flavones has better effect to improving the diabetes disorders of lipid metabolism.See Table 6.
Table 6: the Radix et Rhizoma Gynurae divaricatae total flavones to the influence of T2DM rat fat (n=8,
±
s)
Annotate: compare with the normal control group,
a p＜0.01; Compare with model control group,
b p＜0.05,
c p＜0.01
5.3 the Radix et Rhizoma Gynurae divaricatae total flavones is to the influence of type 2 diabetes mellitus rat model serum insulin
Irritate after stomach finishes, compare with the normal control group, the serum insulin levels of model group rat obviously raise (
p＜0.01), illustrates that hyperinsulinemia has appearred in the type 2 diabetes mellitus rat model; Compare with model control group, the Radix et Rhizoma Gynurae divaricatae total flavones is low, the high dose group serum insulin levels all has reduction in various degree, have significant differences (
p＜0.01).Results suggest Radix et Rhizoma Gynurae divaricatae total flavones has better action to improving type 2 diabetes mellitus rat hyperinsulinemia.Result such as table 7.
Table 7: the Radix et Rhizoma Gynurae divaricatae total flavones to the influence of T2DM rat blood serum insulin (n=8,
±
s)
Annotate: compare with the normal control group,
a p＜0.01; Compare with model control group,
b p＜0.01
5.4 the Radix et Rhizoma Gynurae divaricatae total flavones is to the influence of type 2 diabetes mellitus rat model liver glycogen
Compare with the normal control group, the liver glycogen content of model group rat obviously reduce (
p＜0.01); Compare with model control group, the liver glycogen content of metformin group and glibenclamide group all have in various degree rising (
p＜0.01,
p＜0.05), and the Radix et Rhizoma Gynurae divaricatae total flavones is low, high dose group liver glycogen content is suitable with model group, no significant change (
p0.05).The result is as shown in table 8.
Table 8: the Radix et Rhizoma Gynurae divaricatae total flavones to the influence of T2DM rats'liver glycogen content (n=8,
±
s)
Annotate: compare with the normal control group,
a p＜0.01; Compare with model control group,
b p＜0.05,
c p＜0.01,
d p0.05
5.5 the Radix et Rhizoma Gynurae divaricatae total flavones is to the influence of SOD, MDA in the type 2 diabetes mellitus rat model blood
Compare with the normal control group, superoxide dismutase in the model group rat blood serum (SOD) vigor obviously reduce (
p＜0.01), and dead end product malonaldehyde (MDA) content of lipid peroxidation obviously increase (
p＜0.01), the ability drop of type 2 diabetes mellitus rat model body removing free radical is described, the oxidative stress level raises; And the Radix et Rhizoma Gynurae divaricatae total flavones is low, the high dose group serum activity of SOD is apparently higher than model group, MDA content be starkly lower than model group (
p＜0.01), results suggest Radix et Rhizoma Gynurae divaricatae total flavones can increase the diabetes rat activities of antioxidant enzymes, alleviates lipid peroxidation.Result such as table 9.
Table 9: the Radix et Rhizoma Gynurae divaricatae total flavones to the influence of T2DM rat serum SOD, MDA (n=8,
±
s)
Annotate: compare with the normal control group,
a p＜0.01; Compare with model control group,
b p＜0.01
5.6 the Radix et Rhizoma Gynurae divaricatae total flavones is to the influence of type 2 diabetes mellitus rat model vascular endothelial function
Compare with the normal control group, nitric oxide in the model group rat blood serum (NO) content obviously reduce (
p＜0.01), and the level of endothelin level (ET) obviously increase (
p＜0.01), illustrates that type 2 diabetes mellitus rat model vascular endothelial function is impaired; And the Radix et Rhizoma Gynurae divaricatae total flavones is low, the high dose group serum NO levels is apparently higher than model group, level of ET in plasma be starkly lower than model group (
p＜0.01), results suggest Radix et Rhizoma Gynurae divaricatae total flavones may have the certain protection effect to the vascular endothelial function of diabetes rat.Result such as table 10.
Table 10: the Radix et Rhizoma Gynurae divaricatae total flavones to the influence of type 2 diabetes mellitus rat blood serum NO content, level of ET in plasma (n=8,
±
s)
Annotate: compare with the normal control group,
a p＜0.01; Compare with model control group,
b p＜0.01,
c p＜0.05
6 conclusions
The Radix et Rhizoma Gynurae divaricatae total flavones can significantly reduce type 2 diabetes mellitus rat model blood glucose, improve its disorders of lipid metabolism and hyperinsulinemia, increase the body activities of antioxidant enzymes, alleviate lipid peroxidation, thereby its blood sugar lowering mechanism may be the damage of removing the ability inhibition radical pair beta Cell of islet of reactive oxygen free radical by enhancing body, promotes the reparation of beta Cell of islet.The Radix et Rhizoma Gynurae divaricatae total flavones has the certain protection effect to the damage of T2DM blood vessel endothelium.In sum, the Radix et Rhizoma Gynurae divaricatae total flavones of the present invention's preparation has the effect of significant treatment diabetes.
Claims (6)
1. the preparation method of a Radix et Rhizoma Gynurae divaricatae total flavones, it is characterized in that: get Radix et Rhizoma Gynurae divaricatae, pulverize, add the 40-95%(weight ratio, alcohol reflux down together), extracting solution concentrates the back and goes up macroporous resin column, uses the ethanol water gradient elution, collect 35-55% ethanol elution part, concentrate and obtain total flavones.
2. according to the preparation method of the described Radix et Rhizoma Gynurae divaricatae total flavones of claim 1, it is characterized in that: Radix et Rhizoma Gynurae divaricatae is ground into 40 order powder, use the 45-70% alcohol reflux, extracting solution concentrates the back and goes up the D-101 macroporous resin column, use the ethanol water gradient elution, collect 40-50% alcohol eluting part, concentrate and obtain total flavones.
3. according to the preparation method of the described Radix et Rhizoma Gynurae divaricatae total flavones of claim 2, it is characterized in that: Radix et Rhizoma Gynurae divaricatae is ground into 40 order powder, adopt the 50-80% alcoholic solution of 6-10 times of medical material weight (W/V), reflux, extract, under 80-95 ℃ of condition, extracting solution concentrates the back and goes up the D-101 macroporous resin column, use the ethanol water gradient elution, collect 40-50% ethanol elution part, concentrate and obtain total flavones.
4. according to the preparation method of the described Radix et Rhizoma Gynurae divaricatae total flavones of claim 3, it is characterized in that: Radix et Rhizoma Gynurae divaricatae is ground into 40 order powder, adopt the 50-55% alcoholic solution of 7-9 times of medical material weight (W/V), reflux, extract, is 2 times under 85-90 ℃ of condition, extracting solution concentrates the back and goes up the D-101 macroporous resin column, increase progressively eluting with the ethanol water gradient, collect 40-50% ethanol elution part, concentrate and obtain total flavones.
5. according to the preparation method of the described Radix et Rhizoma Gynurae divaricatae total flavones of claim 4, it is characterized in that: Radix et Rhizoma Gynurae divaricatae is ground into 40 order powder, adopt 50% alcoholic solution of 8 times of medical material weight (W/V), reflux, extract, is 2 times under 85 ℃ of conditions, extracting solution concentrates the back and goes up the D-101 macroporous resin column, use 25%, 45%, 70%, 95% alcoholic solution gradient elution successively, collect 45% ethanol elution, concentrate and obtain total flavones.
6. the application of a Radix et Rhizoma Gynurae divaricatae total flavones that obtains as preparation method as described in the claim 1,2,3,4 or 5 is characterized in that: described total flavones mixes with suitable carrier pharmaceutically, and preparation has the medicine of hypoglycemic activity.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102166238A (en) * | 2011-04-08 | 2011-08-31 | 桂林英美特生物技术有限公司 | Notoginseng extract and application thereof |
| CN102188472A (en) * | 2011-05-12 | 2011-09-21 | 江苏华生安颐生物科技有限公司 | Traditional Chinese medicine application for diminishing inflammation and promoting granules and preparation method thereof |
| CN107853174A (en) * | 2017-10-31 | 2018-03-30 | 安徽新华学院 | A kind of method for improving angelica keiskei koidz lateral bud survival rate |
| CN108771713A (en) * | 2018-07-17 | 2018-11-09 | 佛山市聚成生化技术研发有限公司 | A kind of decompression fat reducing composition and preparation method thereof |
| CN108836988A (en) * | 2018-08-03 | 2018-11-20 | 瑞丽市谊灵草农业发展有限公司 | The preparation method and applications of Gynura divaricata extractive of general flavone |
| JP2020534357A (en) * | 2017-09-18 | 2020-11-26 | ▲ザン▼州片仔▲ファン▼薬業股▲フン▼有限公司 | Flavonoid extract of Fusilier gynura, its preparation method and its use for treating alcoholic fatty liver |
| JP2020537686A (en) * | 2017-09-18 | 2020-12-24 | ▲ザン▼州片仔▲ファン▼薬業股▲フン▼有限公司 | Flavonoid extract of Gynura fusilier, and its preparation method and its use for treating non-alcoholic fatty liver |
| CN114533774A (en) * | 2022-03-16 | 2022-05-27 | 长三角健康农业研究院(浙江)有限公司 | Natural compound for dispelling effects of alcohol and protecting liver and preparation process thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147749A (en) * | 2006-02-21 | 2008-03-26 | 郑乐建 | Divaricate velvetplant medicinal preparation for treating diabetes |
| CN101278957A (en) * | 2007-04-03 | 2008-10-08 | 郑乐建 | Application of extract of Gynura Divaricata in anti-cancer medicine |
| CN100475232C (en) * | 2006-07-17 | 2009-04-08 | 浙江杭州鑫富药业股份有限公司 | Oral medicine for treating diabetes and use thereof |
| CN101485706A (en) * | 2008-01-19 | 2009-07-22 | 郑乐建 | Gynura divaricata (L.) DC. with oxidation-resisting, free radical-resisting and face plaque-removing functions and soft capsule preparation thereof |
-
2010
- 2010-08-25 CN CN 201010261984 patent/CN101953866A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147749A (en) * | 2006-02-21 | 2008-03-26 | 郑乐建 | Divaricate velvetplant medicinal preparation for treating diabetes |
| CN100475232C (en) * | 2006-07-17 | 2009-04-08 | 浙江杭州鑫富药业股份有限公司 | Oral medicine for treating diabetes and use thereof |
| CN101278957A (en) * | 2007-04-03 | 2008-10-08 | 郑乐建 | Application of extract of Gynura Divaricata in anti-cancer medicine |
| CN101485706A (en) * | 2008-01-19 | 2009-07-22 | 郑乐建 | Gynura divaricata (L.) DC. with oxidation-resisting, free radical-resisting and face plaque-removing functions and soft capsule preparation thereof |
Non-Patent Citations (4)
| Title |
|---|
| 《中国博士学位论文全文数据库 医药卫生科技辑》 20091015 陈磊 "白背三七降血糖物质基础研究" E057-114 1-6 , 第10期 2 * |
| 《中国药师》 20091231 王津江 等 "白背三七总黄酮的提取工艺研究" 146-149 1-6 第12卷, 第2期 2 * |
| 《中药现代化》 20020930 赵新先 "中药现代化" 广东世界图书出版公司 113-115 1-6 , 第1版 1 * |
| 《黄酮类化合物研究》 20090131 唐浩国 等 "黄酮类化合物研究" 科学出版社 16，17 1-6 , 第1版 1 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102166238A (en) * | 2011-04-08 | 2011-08-31 | 桂林英美特生物技术有限公司 | Notoginseng extract and application thereof |
| CN102166238B (en) * | 2011-04-08 | 2012-06-20 | 桂林英美特生物技术有限公司 | Notoginseng extract and application thereof |
| CN102188472A (en) * | 2011-05-12 | 2011-09-21 | 江苏华生安颐生物科技有限公司 | Traditional Chinese medicine application for diminishing inflammation and promoting granules and preparation method thereof |
| CN102188472B (en) * | 2011-05-12 | 2012-11-14 | 江苏华生安颐生物科技有限公司 | Traditional Chinese medicine application for diminishing inflammation and promoting granules and preparation method thereof |
| JP2020534357A (en) * | 2017-09-18 | 2020-11-26 | ▲ザン▼州片仔▲ファン▼薬業股▲フン▼有限公司 | Flavonoid extract of Fusilier gynura, its preparation method and its use for treating alcoholic fatty liver |
| JP2020537686A (en) * | 2017-09-18 | 2020-12-24 | ▲ザン▼州片仔▲ファン▼薬業股▲フン▼有限公司 | Flavonoid extract of Gynura fusilier, and its preparation method and its use for treating non-alcoholic fatty liver |
| US11266704B2 (en) | 2017-09-18 | 2022-03-08 | Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd. | Total flavonoid extract from Gynura formosana Kitam., preparation method thereof, and use of same in treating non-alcoholic fatty liver disease |
| JP7166344B2 (en) | 2017-09-18 | 2022-11-07 | ▲ザン▼州片仔▲ファン▼薬業股▲フン▼有限公司 | Flavonoid extract of Pyrophyllum purpurea, and its method of preparation and use in treating alcoholic fatty liver |
| CN107853174A (en) * | 2017-10-31 | 2018-03-30 | 安徽新华学院 | A kind of method for improving angelica keiskei koidz lateral bud survival rate |
| CN108771713A (en) * | 2018-07-17 | 2018-11-09 | 佛山市聚成生化技术研发有限公司 | A kind of decompression fat reducing composition and preparation method thereof |
| CN108836988A (en) * | 2018-08-03 | 2018-11-20 | 瑞丽市谊灵草农业发展有限公司 | The preparation method and applications of Gynura divaricata extractive of general flavone |
| CN114533774A (en) * | 2022-03-16 | 2022-05-27 | 长三角健康农业研究院(浙江)有限公司 | Natural compound for dispelling effects of alcohol and protecting liver and preparation process thereof |
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