CN104523820A - Low-temperature dynamic extraction process of sophora alopecuroide alkaloid extract - Google Patents
Low-temperature dynamic extraction process of sophora alopecuroide alkaloid extract Download PDFInfo
- Publication number
- CN104523820A CN104523820A CN201510021714.5A CN201510021714A CN104523820A CN 104523820 A CN104523820 A CN 104523820A CN 201510021714 A CN201510021714 A CN 201510021714A CN 104523820 A CN104523820 A CN 104523820A
- Authority
- CN
- China
- Prior art keywords
- extraction
- low
- extraction process
- sophora alopecuroide
- oxymatrine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Abstract
The invention provides a low-temperature dynamic extraction process of a sophora alopecuroide alkaloid extract. The process comprises the following steps: a, crushing sophora alopecuroide seeds; and b, extracting with water of which the amount is 8-12 times of the amount of sophora alopecuroide for 1-3 times at a temperature of 45-90 DEG C and vacuum degree of 0.010-0.088MPa for 1-1.5 hours with. The invention further provides the sophora alopecuroide alkaloid extract prepared by the extraction process. Massive repeated testing researches are carried on the low-temperature dynamic extraction process, and the defects of low effective ingredient content and more impurities caused by high temperature of the traditional sophora alopecuroide alkaloid extraction process can be overcome. The extracted sophora alopecuroide alkaloid extract is simple and convenient to operate, small in pollution and low in cost, is advantageous to industrial large-scale production, and provides a research thought to application of traditional Chinese medicine reduced-pressure low-temperature dynamic extraction technology in traditional Chinese medicine extraction.
Description
Technical field
The present invention relates to a kind of low-temperature dynamic extraction process of Alkaloids From Sophora Alopecuroides L extract.
Background technology
Herba Sophorae alopecuroidis belongs to pulse family Sophora, is the natural Chinese medicine with northwest local special features.According to Compendium of Materia Medica record, bitter bean is cold in nature, taste is extremely bitter, poisonous; There is heat-clearing and toxic substances removing, the effects such as anti-inflammation.The chemical composition of Herba Sophorae alopecuroidis is comparatively complicated, is mainly alkaloid, flavonoid, volatile oil, organic acid, aminoacid etc., and alkaloid is then the Main way of research both at home and abroad.Modern medicine study proves, the medical value of Herba Sophorae alopecuroidis is high, its main component alkaloid has the pharmacologically active widely such as immunity moderation function, anti-hepatitis virus, antitumor, therefore has obvious effect to cardiovascular system, immune system, immune system, musculature etc.Herba Sophorae alopecuroidis has better inhibitory action to salmonella paratyphi, staphylococcus aureus; Alkaloids From Sophora Alopecuroides L all has certain inhibitory action to escherichia coli, streptococcus agalactiae, pasteurella multocida.Aloperine has significant inhibitory action to acute inflammation and III, type Ⅳ allergy and the adjuvant arthritis that multiple proinflammatory agent causes.Wherein, oxymatrine has significant anti-hepatitis B, the liver protecting and ALT lowering, has protective effect to multiple hepatic injury, and the matrine tablet preparation researched and developed obtains good clinical efficacy.
Herba Sophorae alopecuroidis is also widely used at food, industry, agriculture field.The compound gel that Sophora Alopecuroides Gum can be used as xanthan gum is produced for food industry; In its seed, the water soluble polysaccharide that contains in endosperm, main component galactomannan, is applied to the industries such as oil, medicine, weaving, food, papermaking as thickening agent, stabilizing agent, gel etc.Aloperine also has good prevention effect to loose ends worm, as the control of formulation application in forestry insect pest; The virulence of cytisine to radish aphid is better than famous killing aphid alkaloid anabasine and nicotine.In addition, Herba Sophorae alopecuroidis relies on its extremely strong vitality, and resistance to dust storm, the characteristic such as weather-proof, Herba Sophorae alopecuroidis has been classified as excellent sand-fixation plant by the Northwest, for checking winds and fixing drifting sand of the Northwest, preserves the ecological environment.
In existing extractive technique, extract total alkaloid of sophora alopecuroide and mainly adopt ethanol refluxing process, sour water infusion process, ultrasonic wave added method, microwave―assisted extraction and supercritical fluid extraction, below all use a large amount of organic solvents, equipment investment amount high, be unfavorable for industrialization.
The research such as Jia Minge shows, in Sophora flavescens, the content of oxymatrine is apparently higher than matrine, its medical material is after soak by water, and the content of oxymatrine, a little more than matrine, finally reaches dynamic equilibrium along with the prolongation oxymatrine of decocting time is converted into matrine trend accordingly.Pan Guangzhou etc. have investigated the impact of the different extraction process of infusion process, ultrasonic method, hot dipping and reflux on the mutual conversion of matrine and oxymatrine, further research finds when extract at room temperature, the content of oxymatrine is far above matrine, and the content that there is oxymatrine after heating reduces, the phenomenon of the content rising of matrine.Experiment all illustrates that temperature is higher in 25-100 DEG C, and the time of heating is longer, and oxymatrine transforms more abundant to matrine.The prompting of this result, when selective extraction technique, answers emphasis to consider influence factor's temperature.
In sum, oxymatrine has unstability, there is the trend transformed to matrine, and temperature is the key factor affecting this conversion process.Treat the significant curative effect of chronic hepatitis based on oxymatrine clinically, therefore when extracting, consider the content as far as possible improving oxymatrine in total alkali.
Summary of the invention
Technical scheme of the present invention there is provided a kind of Alkaloids From Sophora Alopecuroides L extract low-temperature dynamic extraction process.
The invention provides a kind of extraction process of Alkaloids From Sophora Alopecuroides L extract, it comprises the following steps:
A, get Herba Sophorae alopecuroidis seed, pulverize;
B, add 8-12 times amount water extraction, Extracting temperature 45-90 DEG C, vacuum is 0.010 ~ 0.088MPa, extraction time 1-1.5h, extraction time 1-3 time.
Further preferably, it comprises the following steps:
A, get Herba Sophorae alopecuroidis seed, pulverize;
B, add 12 times amount water extraction, Extracting temperature 60 DEG C, vacuum is 0.070MPa, extraction time 1h, extraction time 3 times.
Present invention also offers Alkaloids From Sophora Alopecuroides L extract prepared by this extraction process.
This extract is not less than 7%w/w containing Herba Sophorae alopecuroidis total alkali; 1.6%w/w is not less than containing oxymatrine.Further preferably, this extract is containing Herba Sophorae alopecuroidis total alkali 7.2-7.4%w/w; Containing oxymatrine 1.6%-1.9%w/w.
Sophora alopecuroides bases is principle active component, and wherein oxymatrine at high temperature can transform to matrine, extracts Herba Sophorae alopecuroidis, both can alleviate oxymatrine and transform to matrine according to decompression low-temperature dynamic, again can in boiling dynamically lower high efficiency extraction.The present invention adopts decompression low-temperature dynamic extractive technique, be applied to the extraction of Alkaloids From Sophora Alopecuroides L, not only increase the content of effective ingredient oxymatrine, improve the rate of transform of medical material, save material, relatively low Extracting temperature alleviates its conversion to matrine, and in extracting solution, impurity content greatly reduces simultaneously, obvious reduction paste-forming rate, the concentrated and purified difficulty of effective solution.
Research worker of the present invention is studied through lot of experiments, decompression low-temperature dynamic extraction process is studied repeatedly, overcome tradition extraction Alkaloids From Sophora Alopecuroides L technological temperature high, oxymatrine is caused easily to be converted into the more shortcoming of matrine, impurity, extraction process is easy and simple to handle, it is little to pollute, cost is low, be beneficial to industrialized great production, and applying in Chinese medicine extraction for Chinese medicine decompression extractive technique provides a kind of Research Thinking.
Accompanying drawing explanation
Fig. 1 linear relationship is investigated
The content results of Fig. 2 oxymatrine
The content of Fig. 3 Herba Sophorae alopecuroidis total alkali
Detailed description of the invention
The extraction of embodiment 1 Alkaloids From Sophora Alopecuroides L of the present invention
A, get Herba Sophorae alopecuroidis seed, pulverize;
B, add 12 times amount water extraction, Extracting temperature 60 DEG C, vacuum is 0.070MPa, extraction time 1h, extraction time 3 times.
Embodiment 2 extraction process screening test of the present invention
The assay of 1 Herba Sophorae alopecuroidis total alkali
1.1 preparation 2x10
-4the buffer of mol/L bromothymol blue pH=7.6
Get 0.1mol/L sodium dihydrogen phosphate 100mL, add 0.1mol/L sodium hydroxide solution 84.4mL, distilled water 15.2mL, after mixing, haggle over positive pH=7.6 by ionic acidity.Take bromothymol blue 0.025g to be dissolved in the buffer solution of above-mentioned 200mLpH=7.6, to obtain final product.
1.2 reference substance solution preparation accurately take 10.0mg matrine reference substance, with anhydrous alcohol solution and standardize solution in the volumetric flask of 10mL, to obtain final product.
1.3 need testing solutions are prepared accurately weighed Herba Sophorae alopecuroidis coarse powder and are about 0.1g, are placed in 25mL volumetric flask, add ammonia 2mL, place 30min, and add the vibration of appropriate chloroform, place 2h, add chloroform to scale, room temperature places 12h, and filter, subsequent filtrate makes extracting solution.
The selection precision of 1.4 determined wavelength pipettes matrine reference substance solution and need testing solution in right amount in 25mL tool plug test tube, be placed in solvent evaporated on 80 DEG C of water-baths, add bromothymol blue pH=7.6 buffer solution 5mL, chloroform 6mL, inaccessible violent jolting 2min, proceed in 60mL separatory funnel, leave standstill 2h, after water layer and chloroform layer are distinguished completely, divide and get chloroform layer (yellow) in rub oral examination tube, and being to complying blank experiment with batch chloroform solvent, in 200-600nm wave-length coverage, carrying out UV scanning, determine that maximum absorption wave is 413nm.
1.5 linear relationships investigate precisions measure reference substance solution 20,30,40,50,60,70uL, be placed in the ground conical flask of 25mL respectively, most ethanol is waved in water-bath 80 DEG C, add bromothymol blue pH=7.6 buffer solution 5mL, all the other operate according under " 1.3 " item, not add matrine for blank, measure absorbance respectively in 413nm place.Make linear regression with concentration C (ug/mL) to A value, obtaining standard curve is Y=0.0694X-0.0862, R
2=0.999, good in matrine dependency within the scope of 3.36 ~ 11.7ug/mL, see Fig. 1.
1.6 precision test precisions pipette above-mentioned reference substance solution 60ul, measure A value according under " 1.3 " item, replication 6 times, and RSD=0.23% can obtain the method and have higher precision, stablize feasible.
1.7 stability tests are got and are above-mentionedly prepared sample solution, measure its absorbance once, investigate its absorbance over time, RSD=1.5% every 30min, and its result illustrates that Herba Sophorae alopecuroidis total alkali is highly stable in 3h, is suitable for doing assay test analysis.
1.8 replica tests prepare sample solution according under " 1.4 " item, parallel 6 parts, and each precision measures 0.1mL in tool plug test tube, parallel 6 parts, measures its absorbance, RSD=2.07% according under " 1.3 " item, and its result illustrates that the method repeatability is good.
1.9 average recoveries test accurately weighed 0.1g Herba Sophorae alopecuroidis coarse powder, parallel 3 parts, add appropriate reference substance solution respectively, measure its A value according under " 1.3 " item, calculate average recovery.Result average recovery rate %=100.03%, RSD=2.65%.
The assay of 2 oxymatrines
2.1 chromatographic condition chromatographic columns: Kromasil 100-5C18 post (250nm*4.6mm, 5um); Mobile phase: acetonitrile-0.05mol/L KH
2p0
4(with triethylamine adjust pH to 6.5 scholar 0.2) (8:92); Determined wavelength is 205nm, temperature 30 DEG C, sample size 10uL.
The accurately weighed oxymatrine 10.20mg of preparation of 2.2 reference substance solution puts in 10mL volumetric flask, and dissolve with methanol is also settled to scale, shakes up, and obtains the reference substance solution that concentration is 1.02mg/mL.
2.3 need testing solutions are prepared precision and are taken Herba Sophorae alopecuroidis coarse powder 0.1g and put in 50mL volumetric flask, and add ammonia 1mL, methanol constant volume, to scale, is fully vibrated, and room temperature places 12h, ultrasonic 30min, filter, get subsequent filtrate and get final product with microporous filter membrane (0.45um).
2.4 linear test precisions measure reference substance stock solution 0.1mL, 0.3mL, 0.5mL, 0.7mL, 1.0mL, 1.5mL, put 10mL volumetric flask, add methanol dilution and be settled to scale to shake up.Respectively by above-mentioned chromatographic condition sample introduction 10ul, record the peak area of each reference substance.With peak area (A) for vertical coordinate, Oxymatrine alkali concn (mg/mL) is abscissa drawing standard curve.Obtaining regression equation is Y=1E+07X+51689, R
2=0.9997 within the scope of 0.0102 ~ 0.153mg/mL dependency good.
2.5 precision test precisions draw oxymatrine reference substance solution 10uL, according to above-mentioned chromatographic condition, repeat sample introduction 6 times, and record peak area value, the RSD calculating peak area is 1.09%, and this result shows that instrument precision is good.
2.6 replica test precisions take 6 parts of same batch samples, prepare need testing solution, respectively get lOul injecting chromatograph according under " 2.3 " item, record peak area value, result RSD=1.94%, illustrate that the method repeatability is good.
2.7 solution stability testings are got same need testing solution and are put in ambient temperatare, according to above-mentioned chromatographic condition respectively at 0,2,4,6,8 and 10 hour sample introduction 10ul, record peak area value, calculating peak area is worth RSD=2.79%, and its result shows that sample solution is stable in 10h.
The accurate sample getting appropriate known content of 2.8 average recovery test, the solution that each precision adds matrine and the high, medium and low concentration of oxymatrine reference substance is appropriate, gets 10ul injecting chromatograph, and record chromatogram, calculates average recovery.Result average recovery rate %=99.73%, RSD=2.86%.
3 decompression Study on extraction
3.1 Extracting temperature single factor exploration test list of references report and trial tests, employing water is Extraction solvent.Take Herba Sophorae alopecuroidis coarse powder 6 parts, every part of 20g, add 12 times of water gagings, the low-temperature dynamic that reduces pressure under 40,50,60,70,80,90 DEG C of conditions respectively extracts 2h, extracts 2 times.Adopt colorimetric method for determining Herba Sophorae alopecuroidis total alkali content, HPLC method measures oxymatrine concentration, using total alkali and Oxymatrine content as inspection target, result shows, 60 DEG C of decompression dynamic extraction, in Herba Sophorae alopecuroidis total alkali and oxymatrine extracted amount higher, therefore adopt 60 DEG C decompression dynamic extraction, see Fig. 2, Fig. 3.
Table 1 single factor experiment result
3.2 optimal processing parameter screening tests
Get Herba Sophorae alopecuroidis seed, pulverize; Add 8-12 times amount water extraction respectively, Extracting temperature 60 DEG C, vacuum is 0.070MPa, extraction time 1-2h, and extraction time 1-3 time detects the percentage composition of wherein Herba Sophorae alopecuroidis total alkali and oxymatrine, in table 2:
By table 2 data analysis, wherein the content of the 9th group of Herba Sophorae alopecuroidis total alkali prepared and oxymatrine is significantly higher than other group, therefore, determines that optimum extraction process is: 12 times of water gagings, and 60 DEG C of decompression dynamic extraction 3 times, each 1h, technique is optimum.
3.4 demonstration tests are investigate reliability and the stability of selection process further, get above-mentioned 3 parts of medical materials and carry out demonstration test according to above-mentioned optimum condition.Because considering the convertibility of oxymatrine under optimal extract process, the content increasing matrine is testing index.This result show through orthogonal preferred optimal extract process have operability, reproducible, stablize feasible, be applicable to Sophora alopecuroides alkali components extract.
Table 3 demonstration test result
3.5 reduced-pressure backflow extraction methods and decocting method, the comparing of atmospheric pressure reflux extraction method
Decocting method takes Herba Sophorae alopecuroidis coarse powder 20g, parallel 3 parts, adds 12 times amount water boiling and extraction 1h, totally 3 times; Filter, merging filtrate, evaporate to dryness obtains crude extract, calculates dry spun.With the content of the content of Herba Sophorae alopecuroidis total alkali, oxymatrine and matrine and dry spun for inspection target.
Atmospheric pressure reflux extraction method takes Herba Sophorae alopecuroidis coarse powder 20g, parallel 3 parts, adds 12 times of aqueous solutions respectively, and atmospheric pressure reflux extracts 1h, totally 3 times; Filter, merging filtrate, standardize solution.Get appropriate extracting solution, evaporate to dryness obtains crude extract, calculates dry spun.With the content of the content of Herba Sophorae alopecuroidis total alkali, oxymatrine and matrine and dry spun for inspection target.
Table 4 Different Extraction Method compares (n=3)
From experimental result, the content of decompression dynamic extraction Herba Sophorae alopecuroidis total alkali and oxymatrine comparatively decocting method and atmospheric pressure reflux extracts higher, and more other two kinds of paste-forming rate obviously reduces, and extraction ratio can improve 17%.Especially the dynamic extraction that reduces pressure obviously can weaken oxymatrine to matrine transformation, thus can improve the content of oxymatrine in total alkali.
In sum, research worker of the present invention is studied through lot of experiments, decompression extraction process is studied repeatedly, overcome tradition extraction impurity more, the shortcoming that active constituent content is low, avoid oxymatrine to a certain extent to transform to matrine, improve the ratio of oxymatrine in total alkali, applying in Chinese medicine extraction for Chinese medicine decompression extractive technique provides a kind of Research Thinking.
Claims (5)
1. a low-temperature dynamic extraction process for Alkaloids From Sophora Alopecuroides L extract, is characterized in that: it comprises the following steps:
A, get Herba Sophorae alopecuroidis seed, pulverize;
B, add 8-12 times amount water extraction, Extracting temperature 45-90 DEG C, vacuum is 0.010 ~ 0.088MPa, extraction time 1-1.5h, extraction time 1-3 time.
2. Alkaloids From Sophora Alopecuroides L extraction process according to claim 1, is characterized in that: it comprises the following steps:
A, get Herba Sophorae alopecuroidis seed, pulverize;
B, add 12 times amount water extraction, Extracting temperature 60 DEG C, vacuum is 0.070MPa, extraction time 1h, extraction time 3 times.
3. Alkaloids From Sophora Alopecuroides L extract prepared by the extraction process described in claim 1 or 2.
4. Alkaloids From Sophora Alopecuroides L extract according to claim 3, is characterized in that: be not less than 7%w/w containing Herba Sophorae alopecuroidis total alkali; 1.6%w/w is not less than containing oxymatrine.
5. Alkaloids From Sophora Alopecuroides L extract according to claim 4, is characterized in that: containing Herba Sophorae alopecuroidis total alkali 7.2-7.4%w/w; Containing oxymatrine 1.6%-1.9%w/w.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510021714.5A CN104523820A (en) | 2015-01-15 | 2015-01-15 | Low-temperature dynamic extraction process of sophora alopecuroide alkaloid extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510021714.5A CN104523820A (en) | 2015-01-15 | 2015-01-15 | Low-temperature dynamic extraction process of sophora alopecuroide alkaloid extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104523820A true CN104523820A (en) | 2015-04-22 |
Family
ID=52839576
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510021714.5A Pending CN104523820A (en) | 2015-01-15 | 2015-01-15 | Low-temperature dynamic extraction process of sophora alopecuroide alkaloid extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104523820A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105030889A (en) * | 2015-09-08 | 2015-11-11 | 保定冀中药业有限公司 | Sophora alopecuroides oral liquid and preparing method thereof |
| CN106420907A (en) * | 2016-12-23 | 2017-02-22 | 成都中医药大学 | Sophora alopecuraides alkaloid double-layer suppository and preparation method thereof |
| CN107896839A (en) * | 2017-10-19 | 2018-04-13 | 广西壮族自治区金秀瑶族自治县科学技术情报研究所 | A kind of chrysomelid control method of illiciumverum |
| CN109754164A (en) * | 2018-12-18 | 2019-05-14 | 内蒙古鄂尔多斯资源股份有限公司东昊厂 | A kind of cashmere textile manufacturing calculates the dynamic row yielding model of input amount |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020192256A1 (en) * | 2000-09-06 | 2002-12-19 | Chang-An Wu | Water miscible pesticide containing a synergistic cocktail of plant alkaloids |
| CN103860637A (en) * | 2012-12-17 | 2014-06-18 | 宁夏紫荆花制药有限公司 | Preparing method of total alkali liquor of sophora alopecuroide |
-
2015
- 2015-01-15 CN CN201510021714.5A patent/CN104523820A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020192256A1 (en) * | 2000-09-06 | 2002-12-19 | Chang-An Wu | Water miscible pesticide containing a synergistic cocktail of plant alkaloids |
| CN103860637A (en) * | 2012-12-17 | 2014-06-18 | 宁夏紫荆花制药有限公司 | Preparing method of total alkali liquor of sophora alopecuroide |
Non-Patent Citations (2)
| Title |
|---|
| 梅明等: "苦参常压提取与减压提取的工艺效果比较", 《中国实验方剂学杂志》 * |
| 郭宏昌: "《中草药抗肿瘤便览》", 30 November 2014, 新疆人民卫生出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105030889A (en) * | 2015-09-08 | 2015-11-11 | 保定冀中药业有限公司 | Sophora alopecuroides oral liquid and preparing method thereof |
| CN105030889B (en) * | 2015-09-08 | 2019-05-21 | 保定冀中药业有限公司 | A kind of Sophora alopecuroide oral solution and preparation method thereof |
| CN106420907A (en) * | 2016-12-23 | 2017-02-22 | 成都中医药大学 | Sophora alopecuraides alkaloid double-layer suppository and preparation method thereof |
| CN107896839A (en) * | 2017-10-19 | 2018-04-13 | 广西壮族自治区金秀瑶族自治县科学技术情报研究所 | A kind of chrysomelid control method of illiciumverum |
| CN107896839B (en) * | 2017-10-19 | 2019-11-12 | 广西壮族自治区金秀瑶族自治县科学技术情报研究所 | A kind of control method that illiciumverum is chrysomelid |
| CN109754164A (en) * | 2018-12-18 | 2019-05-14 | 内蒙古鄂尔多斯资源股份有限公司东昊厂 | A kind of cashmere textile manufacturing calculates the dynamic row yielding model of input amount |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ye et al. | Determination of flavonoids in Semen Cuscutae by RP-HPLC | |
| CN102200530B (en) | Method for detecting 33 kinds of narcotic drugs in urine by liquid chromatography-tandem mass spectrometry | |
| CN105738546B (en) | Method for establishing fingerprint of radix curcumae medicinal material and fingerprint thereof | |
| CN104523820A (en) | Low-temperature dynamic extraction process of sophora alopecuroide alkaloid extract | |
| CN102928526A (en) | Method for analyzing content of adenosine and cordycepin in cordyceps militaris by virtue of high performance liquid chromatography (HPLC) | |
| CN106749143A (en) | A kind of method that scopolactone compound is extracted from tobacco | |
| Li et al. | High performance liquid chromatographic assay of saikosaponins from Radix Bupleuri in China | |
| CN104345111A (en) | Determination method for content of multiple active compositions in traditional Chinese medicine composition preparation | |
| CN109730359A (en) | Triterpene compound in a kind of adiantum cpilus-veneris and preparation method thereof and the application in electronic cigarette | |
| Beltrame et al. | A validated higher-performance liquid chromatography method for quantification of cinchonain Ib in bark and phytopharmaceuticals of Trichilia catigua used as Catuaba | |
| CN109438545A (en) | A kind of natural antibacterial compound and preparation method thereof and the application in electronic cigarette | |
| CN103604879A (en) | Detection method of polystictus versicolor and preparation containing polystictus versicolor | |
| CN103344738B (en) | Detection method of nine-component heart-calming particle | |
| CN103344717B (en) | Method for establishing rhizoma bletillae high performance liquid chromatography (HPLC) fingerprint spectrum and standard fingerprint spectrum thereof | |
| CN107748211B (en) | Method for extracting and measuring 5 macamides in maca by using deep eutectic solvent | |
| Ye et al. | Determination of camphor and borneol in Flos Chrysanthemi Indici by UAE and GC-FID | |
| Liu et al. | Optimization of supercritical fluid extraction of dl-tetrahydropalmatine from rhizome of Corydalis yanhusuo WT Wang with orthogonal array design | |
| CN103512975A (en) | Method for analyzing contents of effective substances in Cordyceps martialis fruiting body and residue by HPLC | |
| CN103163271A (en) | Measuring method for residual amount of cnidium lactone in tobacco leaves | |
| CN103424499B (en) | Method for detecting picrasma quassioides alkali content in picrasma quassioides | |
| CN104535513A (en) | Glabrous sarcandra herb extract and detection method of preparation thereof | |
| CN101871924B (en) | Method for synchronous detection of xanthohumol, isoxanthohumol and 8-isopentenylnaringenin in lupulus | |
| CN107632082B (en) | Method for measuring alkaloid components in zanthoxylum armatum medicinal material | |
| CN103175906B (en) | Qualitative and quantitative detection method for each component of validamycin | |
| CN103983711B (en) | A kind of anoectochilus roxburghii glycosides quantitative analysis detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150422 |
|
| RJ01 | Rejection of invention patent application after publication |