CN109438545A - A kind of natural antibacterial compound and preparation method thereof and the application in electronic cigarette - Google Patents
A kind of natural antibacterial compound and preparation method thereof and the application in electronic cigarette Download PDFInfo
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- CN109438545A CN109438545A CN201910008865.5A CN201910008865A CN109438545A CN 109438545 A CN109438545 A CN 109438545A CN 201910008865 A CN201910008865 A CN 201910008865A CN 109438545 A CN109438545 A CN 109438545A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/183—Treatment of tobacco products or tobacco substitutes sterilization, preservation or biological decontamination
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/34—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances containing a carbocyclic ring other than a six-membered aromatic ring
- A24B15/345—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances containing a carbocyclic ring other than a six-membered aromatic ring containing condensed rings
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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- Toxicology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to a kind of natural antibacterial compounds and preparation method thereof and the application in electronic cigarette, belong to technical field of phytochemistry.The compounds of this invention structural formula is as shown in the formula (I):, formula (I).The compounds of this invention is separated from adiantum cpilus-veneris aerial part for the first time, has been determined by nuclear magnetic resonance and measuring method of mass spectrum as triterpene compound, and characterize its specific structure.The compounds of this invention shows good inhibitory activity to potato verticillium wilt pathogen, southern corn leaf blight, alternaria altemata etc., and minimum inhibitory concentration is from 3.125 ~ 12.5ug/mL etc..The compounds of this invention also shows good antifungal activity simultaneously, and for micrococcus lysodeikticus, Bacillus paratyphosus B and Pseudomonas aeruginosa, minimum inhibitory concentration 100ug/mL has a good application prospect.
Description
Technical field
The invention belongs to technical field of phytochemistry, and in particular to a kind of natural antibacterial compound and preparation method thereof with
Application in electronic cigarette.
Background technique
Adiantum cpilus-veneris, Chinese medicine name are venushair fern plant venushair fernAdiantumcapillus-venerisL. herb, point
It is distributed in the ground such as East China, Central-South, southwestern and Hebei, Shanxi, Shaanxi, Gansu.With clearing heat and detoxicating, the effect of promoting diuresis for stranguria.It can use
In cold, fever, cough with lung heat, diarrhea due to damp-heat, dysentery, stranguria with turbid discharge, leukorrhagia, acute mastitis, scrofula, serious case of furuncle, scald, venomous snake bite.This hair
The bright active constituent by the plant carries out system separation, a kind of isolated natural antibacterial compound, while using living
Property test platform, it is found that it has certain activities present to bacterium and fungi, for the active component application provide base
Plinth data.Currently, the compound and its application have no relevant report.
Summary of the invention
In order to solve the deficiencies in the prior art, the present invention provide a kind of natural antibacterial compound and preparation method thereof in electricity
Application in sub- cigarette, the compound show potato verticillium wilt pathogen, southern corn leaf blight, alternaria altemata etc. good
Inhibitory activity, minimum inhibitory concentration is from 3.125 ~ 12.5ug/mL etc..The compounds of this invention also shows anti-well simultaneously
Fungi activity, for micrococcus lysodeikticus, Bacillus paratyphosus B and Pseudomonas aeruginosa, minimum inhibitory concentration 100ug/mL, tool
There is good application prospect.
To achieve the above object, The technical solution adopted by the invention is as follows:
Unless otherwise indicated, percentage employed in the present invention is mass percent.
A kind of natural antibacterial compound, structural formula are as shown in the formula (I):
, formula (I).
English entitled 3 β, 4 α, 25-Trihydroxyfilican, molecular formula C30H52O3, Chinese is named as adiantum cpilus-veneris element C.
Present invention simultaneously provides the preparation methods of above-mentioned natural antibacterial compound, include the following steps:
Step (1), medicinal extract extract: dry adiantum cpilus-veneris aerial part being crushed to 30 ~ 50 mesh, with concentration of volume percent for 70
~ 95% ethanol water is Extraction solvent, and room temperature is extracted, extracting solution is filtered, filtrate decompression is taken to be concentrated, obtains medicinal extract；
Step (2), silica gel column chromatography: silica gel column chromatography on the medicinal extract that step (1) is obtained, dress column silica gel are 200 ~ 300 mesh, with
Petroleum ether and acetone mixed organic solvents carry out gradient elution；The gradient eluent of each gradient and concentration are collected, is monitored through TLC,
Merge identical part, obtains seven parts A1-A7；
Step (3), gel chromatography separation: being 1:1 chloroform and methanol mixed organic solvents as eluant, eluent using volume ratio, using solidifying
Glue chromatography elutes the part A5 in step (2), obtains tetra- parts B1-B5；To the obtained part B3 again using high pressure liquid
Phase chromatographic separation and purification obtains natural antibacterial compound described in formula (I).
It is further preferred that in step (1), the volume of ethanol water and dry bristles meadow when extracting every time
Upper part mass ratio is 50L:25Kg.
It is further preferred that room temperature extraction time is 2 ~ 4 times, every time 6 ~ 10h in step (1).
It is further preferred that in step (2), the volume ratio of gradient elution be followed successively by 10:1,10:3,10:5,10:6,
10:7,10:9 and 10:10 replace next gradient elution after each gradient elution to TLC contact plate is without point.
It is further preferred that the medicinal extract that step (1) obtains before through silica gel column chromatography, is measured with 1.5 ~ 4 times of medicinal extract weight
Concentration of volume percent be 95% methanol aqueous solution dissolve, then use 2 ~ 4 times of medicinal extract quality of 100 mesh silica gel mixed samples, later
Loading.
It is further preferred that dress column used silica gel weight is 3 ~ 5 times of medicinal extract weight in step (2).
It is further preferred that gel chromatography uses Sephadex LH-20 gel column in step (3).
It is further preferred that it is to use 21.2mm × 150mm that high pressure liquid chromatography, which isolates and purifies, in step (3), 5μm
C18Chromatographic column, using methanol-water as mobile phase, the volume ratio of methanol and water is 65:35, flow velocity 10mL/min, ultraviolet detection
Device Detection wavelength is 254nm, each sample introduction 20μL collects the chromatographic peak of 12min sample, is evaporated after repeatedly adding up, obtains formula (I)
The natural antibacterial compound.
The compound structure that the above method is prepared is measured by the following method:
3 β, 4 α, 25-trihydroxyfilican (1): white amorphous powder: [α]20.4 D +2.0° (c 0.1,
MeOH)；IR (KBr)ν max: 3395 (OH), 2946,1468,1452,1377,1055,1034 cm-1；1H NMR
(CDCl3, 400 MHz) and13C NMR (CDCl3, 100 MHz) and it is shown in Table 1, Fig. 1 and Fig. 2；1H,1H COSY is related to HMBC
Such as Fig. 3, NOESY correlation such as Fig. 4；positive HRESIMSm/z 483.3806 [M+Na]+ (calcd for
C30H52O3Na+, 483.3809)。
Table 1
In addition, the present invention also provides above-mentioned natural antibacterial compounds as the application for preparing antibacterial agent.It is specific antimycotic and anti-thin
Bacterium result is as shown in table 2 and table 3.
2 compound of table antibacterial activity (MIC,μg/mL)
3 compound of table antifungal activity (MIC,μg/mL)
The application that the present invention also provides natural antibacterial compounds in tobacco juice for electronic smoke.The compound is used in tobacco juice for electronic smoke,
There can not only be good antibacterial action, can increase the shelf-life of tobacco juice for electronic smoke, moreover it is possible to reduce the sweet sense in aspiration procedure.
Compared with prior art, the present invention has the advantages that:
Natural antibacterial compound of the present invention is separated from adiantum cpilus-veneris aerial part for the first time, passes through nuclear magnetic resonance and matter
Spectrum measuring method has determined as triterpene compound, and characterizes its specific structure.The compounds of this invention is to potato verticillium wilt
Bacterium, southern corn leaf blight, alternaria altemata etc. show good inhibitory activity, minimum inhibitory concentration from 3.125 ~
12.5ug/mL differ.The compounds of this invention also shows good antifungal activity simultaneously, for micrococcus lysodeikticus, B-mode pair
Typhoid bacillus and Pseudomonas aeruginosa, minimum inhibitory concentration 100ug/mL, have a good application prospect.Meanwhile of the present inventionization
Object is closed for that there can not only be good antibacterial action, can increase the shelf-life of tobacco juice for electronic smoke, moreover it is possible to drop in tobacco juice for electronic smoke
Sweet sense in low aspiration procedure, application easy to spread.
Detailed description of the invention
Fig. 1 be the compounds of this invention (I) nuclear magnetic resonance spectroscopy (1HNMR) figure；
Fig. 2 be the compounds of this invention (I) carbon-13 nmr spectra (13C NMR) figure；
Fig. 3 is the compounds of this invention (I's)1H,1H COSY is related to HMBC；Wherein, arrow HMBC, overstriking straight line are1H,1H COSY
Fig. 4 is that the NOESY of the compounds of this invention (I) is related.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright range.In the examples where no specific technique or condition is specified, described technology or conditions according to the literature in the art
Or it is carried out according to product description.Production firm person is not specified in material therefor or equipment, is that can be obtained by purchase
Conventional products.
Adiantum cpilus-veneris aerial part used in the present invention is common commercially available dried product, and place of production Dali is aqueous for its
Rate is not specifically limited.
For the present invention unless otherwise stated, percentage is mass percent, ratio is mass ratio.
When the present invention is separated using silica gel column chromatography, it can also be tracked simultaneously using TLC thin-layer chromatography contact plate.
Embodiment 1
A kind of preparation method of natural antibacterial compound, includes the following steps:
Step (1), medicinal extract extract: dry adiantum cpilus-veneris aerial part being crushed to 30 ~ 50 mesh, is with concentration of volume percent
70% ethanol water is Extraction solvent, and room temperature is extracted, extracting solution is filtered, filtrate decompression is taken to be concentrated, obtains medicinal extract；
Step (2), silica gel column chromatography: silica gel column chromatography on the medicinal extract that step (1) is obtained, dress column silica gel are 200 ~ 300 mesh, with
Petroleum ether and acetone mixed organic solvents carry out gradient elution；The gradient eluent of each gradient and concentration are collected, is monitored through TLC,
Merge identical part, obtains seven parts A1-A7；
Step (3), gel chromatography separation: being 1:1 chloroform and methanol mixed organic solvents as eluant, eluent using volume ratio, using solidifying
Glue chromatography elutes the part A5 in step (2), obtains tetra- parts B1-B5；To the obtained part B3 again using high pressure liquid
Phase chromatographic separation and purification obtains natural antibacterial compound described in formula (I).
Embodiment 2
A kind of preparation method of natural antibacterial compound, includes the following steps:
Step (1), medicinal extract extract: dry adiantum cpilus-veneris aerial part being crushed to 30 ~ 50 mesh, is with concentration of volume percent
95% ethanol water is Extraction solvent, and room temperature is extracted, extracting solution is filtered, filtrate decompression is taken to be concentrated, obtains medicinal extract；
Step (2), silica gel column chromatography: silica gel column chromatography on the medicinal extract that step (1) is obtained, dress column silica gel are 200 ~ 300 mesh, with
Petroleum ether and acetone mixed organic solvents carry out gradient elution；The gradient eluent of each gradient and concentration are collected, is monitored through TLC,
Merge identical part, obtains seven parts A1-A7；
Step (3), gel chromatography separation: being 1:1 chloroform and methanol mixed organic solvents as eluant, eluent using volume ratio, using solidifying
Glue chromatography elutes the part A5 in step (2), obtains tetra- parts B1-B5；To the obtained part B3 again using high pressure liquid
Phase chromatographic separation and purification obtains natural antibacterial compound described in formula (I).
In step (1), the volume of ethanol water is with dry adiantum cpilus-veneris aerial part mass ratio when extracting every time
50L:25Kg.Room temperature extraction time is 4 times, each 10h.
In step (2), the volume ratio of gradient elution is followed successively by 10:1,10:3,10:5,10:6,10:7,10:9 and 10:10,
Each gradient elution to TLC contact plate without point after, replace next gradient elution.The medicinal extract that step (1) obtains is through silica gel column chromatography
Before, the methanol aqueous solution that the concentration of volume percent measured with 4 times of medicinal extract weight is 95% dissolves, then with 4 times of medicinal extract quality
100 mesh silica gel mixed samples, later loading.Filling column used silica gel weight is 5 times of medicinal extract weight.
In step (3), gel chromatography uses Sephadex LH-20 gel column.
It is to use 21.2mm × 150mm that high pressure liquid chromatography, which isolates and purifies, 5μThe C of m18Chromatographic column is flowing with methanol-water
The volume ratio of phase, methanol and water is 65:35, and flow velocity 10mL/min, UV detector Detection wavelength is 254nm, each sample introduction
20μL collects the chromatographic peak of 12min sample, is evaporated after repeatedly adding up, obtains natural antibacterial compound described in formula (I).
Embodiment 3
A kind of preparation method of natural antibacterial compound, includes the following steps:
Step (1), medicinal extract extract: dry adiantum cpilus-veneris aerial part being crushed to 30 ~ 50 mesh, is with concentration of volume percent
80% ethanol water is Extraction solvent, and room temperature is extracted, extracting solution is filtered, filtrate decompression is taken to be concentrated, obtains medicinal extract；
Step (2), silica gel column chromatography: silica gel column chromatography on the medicinal extract that step (1) is obtained, dress column silica gel are 200 ~ 300 mesh, with
Petroleum ether and acetone mixed organic solvents carry out gradient elution；The gradient eluent of each gradient and concentration are collected, is monitored through TLC,
Merge identical part, obtains seven parts A1-A7；
Step (3), gel chromatography separation: being 1:1 chloroform and methanol mixed organic solvents as eluant, eluent using volume ratio, using solidifying
Glue chromatography elutes the part A5 in step (2), obtains tetra- parts B1-B5；To the obtained part B3 again using high pressure liquid
Phase chromatographic separation and purification obtains natural antibacterial compound described in formula (I).
In step (1), the volume of ethanol water is with dry adiantum cpilus-veneris aerial part mass ratio when extracting every time
50L:25Kg.Room temperature extraction time is 2 times, each 6h.
In step (2), the volume ratio of gradient elution is followed successively by 10:1,10:3,10:5,10:6,10:7,10:9 and 10:10,
Each gradient elution to TLC contact plate without point after, replace next gradient elution.The medicinal extract that step (1) obtains is through silica gel column chromatography
Before, the methanol aqueous solution that the concentration of volume percent measured with 1.5 times of medicinal extract weight is 95% dissolves, then with 2 times of medicinal extract quality
100 mesh silica gel mixed samples, later loading.Filling column used silica gel weight is 3 times of medicinal extract weight.
In step (3), gel chromatography uses Sephadex LH-20 gel column.
It is to use 21.2mm × 150mm that high pressure liquid chromatography, which isolates and purifies, 5μThe C of m18Chromatographic column is flowing with methanol-water
The volume ratio of phase, methanol and water is 65:35, and flow velocity 10mL/min, UV detector Detection wavelength is 254nm, each sample introduction
20μL collects the chromatographic peak of 12min sample, is evaporated after repeatedly adding up, obtains natural antibacterial compound described in formula (I).
Embodiment 4
A kind of preparation method of natural antibacterial compound, includes the following steps:
Step (1), medicinal extract extract: dry adiantum cpilus-veneris aerial part being crushed to 30 ~ 50 mesh, is with concentration of volume percent
90% ethanol water is Extraction solvent, and room temperature is extracted, extracting solution is filtered, filtrate decompression is taken to be concentrated, obtains medicinal extract；
Step (2), silica gel column chromatography: silica gel column chromatography on the medicinal extract that step (1) is obtained, dress column silica gel are 200 ~ 300 mesh, with
Petroleum ether and acetone mixed organic solvents carry out gradient elution；The gradient eluent of each gradient and concentration are collected, is monitored through TLC,
Merge identical part, obtains seven parts A1-A7；
Step (3), gel chromatography separation: being 1:1 chloroform and methanol mixed organic solvents as eluant, eluent using volume ratio, using solidifying
Glue chromatography elutes the part A5 in step (2), obtains tetra- parts B1-B5；To the obtained part B3 again using high pressure liquid
Phase chromatographic separation and purification obtains natural antibacterial compound described in formula (I).
In step (1), the volume of ethanol water is with dry adiantum cpilus-veneris aerial part mass ratio when extracting every time
50L:25Kg.Room temperature extraction time is 3 times, each 8h.
In step (2), the volume ratio of gradient elution is followed successively by 10:1,10:3,10:5,10:6,10:7,10:9 and 10:10,
Each gradient elution to TLC contact plate without point after, replace next gradient elution.The medicinal extract that step (1) obtains is through silica gel column chromatography
Before, the methanol aqueous solution that the concentration of volume percent measured with 2 times of medicinal extract weight is 95% dissolves, then with 3 times of medicinal extract quality
100 mesh silica gel mixed samples, later loading.Filling column used silica gel weight is 4 times of medicinal extract weight.
In step (3), gel chromatography uses Sephadex LH-20 gel column.
It is to use 21.2mm × 150mm that high pressure liquid chromatography, which isolates and purifies, 5μThe C of m18Chromatographic column is flowing with methanol-water
The volume ratio of phase, methanol and water is 65:35, and flow velocity 10mL/min, UV detector Detection wavelength is 254nm, each sample introduction
20μL collects the chromatographic peak of 12min sample, is evaporated after repeatedly adding up, obtains natural antibacterial compound described in formula (I).
Embodiment 5
The identification of --- --- compound structure
The structure for the compound that 1 method of embodiment is prepared is measured by the following method:
White amorphous powder；
IR (KBr) ν max: 3395 (OH), 2946,1468,1452,1377,1055,1034 cm-1；
1 H NMR (CDCl3, 400 MHz) and13C NMR (CDCl3, 100 MHz) and it is shown in Table 1, Fig. 1 and Fig. 2；1H,1H
COSY is related to HMBC such as Fig. 3, NOESY correlation such as Fig. 4
positive HRESIMS m/z 483.3806 [M+Na]+ (calcd for C30H52O3Na+, 483.3809)。
So far, the structure of compound is determined, shown compound shown in formula (I).
Embodiment 6
The compound of Example 2-4 preparation is white amorphous powder.Measuring method is same as Example 5, confirms embodiment
Shown compound shown in the compound formula (I) of 2-4 preparation.
Application examples
The antimycotic and antibacterium of natural antibacterial compound of the present invention is tested, as a result as shown in table 4 and table 5.
4 compound of table antibacterial activity (MIC,μg/mL)
5 compound of table antifungal activity (MIC,μg/mL)
Application of the natural antibacterial compound of the present invention in tobacco juice for electronic smoke.The compound is used in tobacco juice for electronic smoke, can not only
With good antibacterial action, the shelf-life of tobacco juice for electronic smoke can increase, moreover it is possible to the sweet sense in aspiration procedure is reduced, not to sense
Official has adverse effect.Sensory evaluating smoking's result such as table 6, wherein control group is commercially available electronic cigarette liquid product, the city embodiment Zu Wei
Addition embodiment product in tobacco juice for electronic smoke is sold, additional amount is 2%(mass percent).
6 sensory evaluating smoking's result of table
The basic principles, main features and advantages of the present invention have been shown and described above.The technical staff of the industry
It should be appreciated that the present invention is not limited to the above embodiments, the above embodiments and description only describe the present invention
Principle, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these variation and
Improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent
Object defines.
Claims (10)
1. a kind of natural antibacterial compound, which is characterized in that structural formula is as shown in the formula (I):
, formula (I).
2. the preparation method of natural antibacterial compound described in claim 1, which comprises the steps of:
Step (1), medicinal extract extract: dry adiantum cpilus-veneris aerial part being crushed to 30 ~ 50 mesh, with concentration of volume percent for 70
~ 95% ethanol water is Extraction solvent, and room temperature is extracted, extracting solution is filtered, filtrate decompression is taken to be concentrated, obtains medicinal extract；
Step (2), silica gel column chromatography: silica gel column chromatography on the medicinal extract that step (1) is obtained, dress column silica gel are 200 ~ 300 mesh, with
Petroleum ether and acetone mixed organic solvents carry out gradient elution；The gradient eluent of each gradient and concentration are collected, is monitored through TLC,
Merge identical part, obtains seven parts A1-A7；
Step (3), gel chromatography separation: being 1:1 chloroform and methanol mixed organic solvents as eluant, eluent using volume ratio, using solidifying
Glue chromatography elutes the part A5 in step (2), obtains tetra- parts B1-B5；To the obtained part B3 again using high pressure liquid
Phase chromatographic separation and purification obtains natural antibacterial compound described in formula (I).
3. the preparation method of natural antibacterial compound according to claim 1, which is characterized in that in step (1), mention every time
The volume of ethanol water and dry adiantum cpilus-veneris aerial part mass ratio are 50L:25Kg when taking.
4. the preparation method of the triterpene compound according to claim 1 with antibacterial functions, which is characterized in that step
(1) in, room temperature extraction time is 2 ~ 4 times, every time 6 ~ 10h.
5. the preparation method of natural antibacterial compound according to claim 1, which is characterized in that in step (2), gradient is washed
De- volume ratio is followed successively by 10:1,10:3,10:5,10:6,10:7,10:9 and 10:10, each gradient elution to TLC contact plate without
After point, next gradient elution is replaced.
6. the preparation method of natural antibacterial compound according to claim 1, which is characterized in that in step (2), step
(1) medicinal extract obtained is before through silica gel column chromatography, the methanol for being 95% with the concentration of volume percent of 1.5 ~ 4 times of medicinal extract weight amounts
Then aqueous dissolution uses 2 ~ 4 times of medicinal extract quality of 100 mesh silica gel mixed samples, later loading；Dress column used silica gel weight is medicinal extract
3 ~ 5 times of weight.
7. the preparation method of natural antibacterial compound according to claim 1, which is characterized in that in step (3), gel color
Spectrum uses Sephadex LH-20 gel column.
8. the preparation method of natural antibacterial compound according to claim 1, which is characterized in that in step (3), high pressure liquid
Phase chromatographic separation and purification is to use 21.2mm × 150mm, 5μThe C of m18Chromatographic column, using methanol-water as mobile phase, methanol and water
Volume ratio is 65:35, and flow velocity 10mL/min, UV detector Detection wavelength is 254nm, each sample introduction 20μL collects 15min
The chromatographic peak of sample is evaporated after repeatedly adding up, obtains natural antibacterial compound described in formula (I).
9. natural antibacterial compound described in claim 1 is as the application for preparing antibacterial agent.
10. application of the natural antibacterial compound described in claim 1 in tobacco juice for electronic smoke.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110256222A (en) * | 2019-07-03 | 2019-09-20 | 云南中烟工业有限责任公司 | A kind of polyphenol compound and preparation method thereof and the application in electronic cigarette |
| CN110272335A (en) * | 2019-07-03 | 2019-09-24 | 云南中烟工业有限责任公司 | Polyphenol compound monomer and preparation method thereof and the application in electronic cigarette in a kind of periploca spium skin |
| CN110272334A (en) * | 2019-07-03 | 2019-09-24 | 云南中烟工业有限责任公司 | A kind of natural antibacterial polyphenol compound and preparation method thereof and the application in electronic cigarette |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108129544A (en) * | 2018-02-01 | 2018-06-08 | 云南中烟工业有限责任公司 | A kind of compound with antimicrobial acivity and its preparation method and application |
| CN108358991A (en) * | 2018-02-01 | 2018-08-03 | 云南中烟工业有限责任公司 | It is a kind of it is clear it is wide spend in triterpene compound and its preparation method and application |
| CN108774114A (en) * | 2018-08-16 | 2018-11-09 | 云南中烟工业有限责任公司 | A kind of luxuriant and rich with fragrance class compound and the preparation method and application thereof in rush |
| CN108892606A (en) * | 2018-08-16 | 2018-11-27 | 云南中烟工业有限责任公司 | A kind of condensed-nuclei aromatics class compound and the preparation method and application thereof with antibacterial functions |
| CN108911953A (en) * | 2018-08-16 | 2018-11-30 | 云南中烟工业有限责任公司 | A kind of luxuriant and rich with fragrance class compound and the preparation method and application thereof derived from natural plants |
| CN109503696A (en) * | 2019-01-04 | 2019-03-22 | 云南中烟工业有限责任公司 | A kind of triterpene compound with antibacterial functions and preparation method thereof and the application in electronic cigarette |
| CN109730359A (en) * | 2019-01-04 | 2019-05-10 | 云南中烟工业有限责任公司 | Triterpene compound in a kind of adiantum cpilus-veneris and preparation method thereof and the application in electronic cigarette |
| CN110272334A (en) * | 2019-07-03 | 2019-09-24 | 云南中烟工业有限责任公司 | A kind of natural antibacterial polyphenol compound and preparation method thereof and the application in electronic cigarette |
-
2019
- 2019-01-04 CN CN201910008865.5A patent/CN109438545A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108129544A (en) * | 2018-02-01 | 2018-06-08 | 云南中烟工业有限责任公司 | A kind of compound with antimicrobial acivity and its preparation method and application |
| CN108358991A (en) * | 2018-02-01 | 2018-08-03 | 云南中烟工业有限责任公司 | It is a kind of it is clear it is wide spend in triterpene compound and its preparation method and application |
| CN108774114A (en) * | 2018-08-16 | 2018-11-09 | 云南中烟工业有限责任公司 | A kind of luxuriant and rich with fragrance class compound and the preparation method and application thereof in rush |
| CN108892606A (en) * | 2018-08-16 | 2018-11-27 | 云南中烟工业有限责任公司 | A kind of condensed-nuclei aromatics class compound and the preparation method and application thereof with antibacterial functions |
| CN108911953A (en) * | 2018-08-16 | 2018-11-30 | 云南中烟工业有限责任公司 | A kind of luxuriant and rich with fragrance class compound and the preparation method and application thereof derived from natural plants |
| CN109503696A (en) * | 2019-01-04 | 2019-03-22 | 云南中烟工业有限责任公司 | A kind of triterpene compound with antibacterial functions and preparation method thereof and the application in electronic cigarette |
| CN109730359A (en) * | 2019-01-04 | 2019-05-10 | 云南中烟工业有限责任公司 | Triterpene compound in a kind of adiantum cpilus-veneris and preparation method thereof and the application in electronic cigarette |
| CN110272334A (en) * | 2019-07-03 | 2019-09-24 | 云南中烟工业有限责任公司 | A kind of natural antibacterial polyphenol compound and preparation method thereof and the application in electronic cigarette |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110256222A (en) * | 2019-07-03 | 2019-09-20 | 云南中烟工业有限责任公司 | A kind of polyphenol compound and preparation method thereof and the application in electronic cigarette |
| CN110272335A (en) * | 2019-07-03 | 2019-09-24 | 云南中烟工业有限责任公司 | Polyphenol compound monomer and preparation method thereof and the application in electronic cigarette in a kind of periploca spium skin |
| CN110272334A (en) * | 2019-07-03 | 2019-09-24 | 云南中烟工业有限责任公司 | A kind of natural antibacterial polyphenol compound and preparation method thereof and the application in electronic cigarette |
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