CN105027733A - Method for promoting seed germination of taxus Chinensis var. mairei - Google Patents
Method for promoting seed germination of taxus Chinensis var. mairei Download PDFInfo
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- CN105027733A CN105027733A CN201510451483.1A CN201510451483A CN105027733A CN 105027733 A CN105027733 A CN 105027733A CN 201510451483 A CN201510451483 A CN 201510451483A CN 105027733 A CN105027733 A CN 105027733A
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Abstract
The invention relates to a method for promoting seed germination of taxus Chinensis var. mairei. The method comprises the following steps: seed collection, concentrated sulfuric acid treatment, seed stratification treatment, MgCl2 treatment, plant lytic enzyme treatment and the like. According to the method, the dormant period of taxus Chinensis var. mairei is greatly shortened, and the germination percentage and the germination vigor of taxus Chinensis var. mairei are greatly improved. The method is simple in whole operation and easy to master, thereby being wide in application prospect.
Description
Technical field
The invention belongs to plant seed process field, be specifically related to a kind of method promoting Germination of Taxus mairei Seeds.
Background technology
Southern enqlish yew is taxaceae Chinese yew genus plants, is extensively distributed in area, the Northern Hemisphere such as Europe, North America and East Asia, domestic be mainly distributed in China Yangtze river basin and on the south each province.
Taxus chinensis var. mairei Seeds has deep dormancy, the germination inhicbitor, temperature etc. of the permeability of seed coat, the developmental state of embryo, seed affect it and sprout, needed for could sprout two summers in winter one under natural conditions, without the morphological maturity seed of specially treated, general need more than 1 year stratification can breaking dormancy, germination rate is low and irregular, often causes the significant wastage of limited seed resource.When southern enqlish yew exists intact seed coats, seed is in obvious anaerobic respiration state, and under height anoxia condition, can cause the formation of mortifier in seed, thus affect physiology after-ripening and the sprouting of seed.Because southern enqlish yew has higher ornamental value, very popular, so promote its seed germination, the speed tool accelerating its breeding is of great significance.
At present, for promoting that the research of Germination of Taxus mairei Seeds has had very large progress,
The present invention is by Seed Germination Test, and the method adopting physics and chemistry process to combine, solves the problem that Germination of Taxus mairei Seeds rate is low.
Application number be 201110113040.3 Chinese invention patent disclose a kind of method breaking Taxus chinensis var. mairei Seeds dormancy, specifically utilize the seed of intermittent warming southern enqlish yew, comprise the steps: a, immersion; B, storage; C, sowing.Achieved by the method and sow emergence rate then and can reach 30%.Second Year can also emerge about 50% again, improves breeding efficiency.Technical problems such as although such scheme improve Germination of Taxus mairei Seeds speed and germination rate to a certain extent, and still there is operation comparatively loaded down with trivial details, cost is higher, and germination rate is lower.Application number be 201210201360.9 Chinese invention patent disclose a kind of method breaking Taxus chinensis var. mairei Seeds dormancy fast and comprise: (1) seed exosper is peeled off: utilize mechanical broken shell, peel manually is from exosper; (2) explant sterilization: successively through 70% alcohol disinfecting 90s and 0.1% mercury chloride sterilization 12min, for subsequent use after aseptic water washing 5 times; (3) Seeds preprocess: with sterile water sealing normal temperature seed soaking 1-5 days; (4) zygotic embryo is separated; (5) cultured in vitro of test-tube plantlet: embryo is inoculated on test-tube plantlet inducing culture, the seed germination rate of 99.9% can be obtained.Although this method effectively improves germination rate, need seed exosper to peel off, obviously operation, easily cause embryo damaged in seed exosper stripping process, whole process wastes time and energy.
Authorization Notice No. is adopt in the Chinese invention patent of a CN103202127B literary composition of " abolishing the method for Taxus chinensis var. mairei Seeds dormancy " to process it with the following method: it is in the sulfuric acid of 55 ~ 65% 2 ~ 4 hours that son is soaked in mass concentration, with running water 12 ~ 36 hours after taking out; Then the Taxus chinensis var. mairei Seeds after acid etching to be soaked in the pre-configured mixed solution containing sprouting stimulin and the basic element of cell division at least 3 hours, after taking-up, in clear water, to soak at least 3 days; Finally again the Taxus chinensis var. mairei Seeds after immersion is carried out the method for cold stratification or alternating temperature stratification process to break the dormancy of seed.This method adopts the method for dilute sulphuric acid to process it, and the processing time is longer, easily seed is excessively soaked destruction seed.
Summary of the invention
The present invention is directed to some problems in currently available technology, provide a kind of method promoting Germination of Taxus mairei Seeds.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: provide a kind of method promoting Germination of Taxus mairei Seeds, comprise the steps:
(1) collection of seed: when stripping out after Taxus chinensis var. mairei Seeds maturation, selects the seed that growth is full, rinses well, naturally dry with the water of flowing;
(2) dense sulfuric acid treatment: the seed after step (1) process is soaked in the concentrated sulfuric acid, process 10 ~ 30s, with running water 30 ~ 60min after taking out;
(3) earthing of seeds process: mixed with wet sand by Taxus chinensis var. mairei Seeds, after plastic bag sealing, be placed in 4 DEG C of refrigerator low temperature treatment, takes out after 20 ~ 25d and cleans;
(4) MgCl
2process: the MgCl Taxus chinensis var. mairei Seeds processed through step (3) being put into 60 ~ 90mg/L
220 ~ 30h is soaked in solution;
(5) plant catabolic enzyme process: it is in the plant catabolic enzyme solution of 6 ~ 8% that the seed after step (4) process is immersed in concentration, processes 20 ~ 24h under the constant temperature of 20 DEG C, takes out and cleans;
(6) germinate and cultivate: the Taxus chinensis var. mairei Seeds processed through step (5) is placed in the culture dish that diameter is 10 ~ 16cm, sprouts relenting under alternate illumination condition, using 24 hours as a cycle period, cultivate 20 ~ 30 days.
In the method for aforementioned promotion Germination of Taxus mairei Seeds, in earthing of seeds processing procedure described in step (3), seed is 1: 3 with the wet husky volume ratio mixed, described wet sand will through disinfecting, concrete sterilization method soaks 10 minutes with the sodium hypochlorite solution that mass percentage concentration is 1%, and then with distilled water flushing extremely without chlorine taste.
In the method for aforementioned promotion Germination of Taxus mairei Seeds, the condition of the alternate illumination that relents described in step (6) is: every day first processes 12h under 25 DEG C of illumination conditions, then processes 12 hours under 15 DEG C of dark conditions.
Beneficial effect of the present invention:
(1) the present invention adopts the concentrated sulfuric acid without dilution to carry out fast processing to it, does not need to dilute the concentrated sulfuric acid, reduces the operating time, can also improve seed coat gas permeability fast, accelerates nutriment and transforms.
(2) MgCl of 60 ~ 90mg/L is adopted
2solution processes, and shortens the time of dormancy.
(2) plant catabolic enzyme is a kind of protein mixture with the decomposition function of organization such as plant cell wall, cell membrane, mainly comprises cellulolytic enzyme, pectin hydrolase etc.There is the performance of general protein, also there is the specificity as a kind of enzyme simultaneously.The inventive method is simple to operate, cost is low, without dangerous, be easy to manual operation, pollution-free, be easy to the advantages such as popularization, the seed seedling-raising of southern enqlish yew, large-scale planting are of very high actual application value.
(4) effectively can be promoted sprouting and the seedling of Taxus chinensis var. mairei Seeds by the process of the inventive method, sprout required time and greatly shorten, germination rate also brings up to 80 ~ 90%.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
The embodiment of the present invention comprises:
Embodiment 1
Promote a method for Germination of Taxus mairei Seeds, comprise the steps:
(1) collection of seed: when stripping out after Taxus chinensis var. mairei Seeds maturation, selects the seed that growth is full, rinses well, naturally dry with the water of flowing;
(2) dense sulfuric acid treatment: the seed after step (1) process is soaked in the concentrated sulfuric acid, process 10s, uses running water 30min after taking out;
(3) earthing of seeds process: first wet sand is disinfected, concrete sterilization method soaks 10 minutes with the sodium hypochlorite solution that mass percentage concentration is 1%, and then with distilled water flushing extremely without chlorine taste.Then be 1: 3 mix with wet husky with volume ratio by Taxus chinensis var. mairei Seeds, after plastic bag sealing, be placed in 4 DEG C of refrigerator low temperature treatment, take out clean after 20d;
(4) MgCl
2process: the MgCl Taxus chinensis var. mairei Seeds processed through step (3) being put into 90mg/L
220h is soaked in solution;
(5) plant catabolic enzyme process: it is in the plant catabolic enzyme solution of 6% that the seed after step (4) process is immersed in concentration, processes 24h under the constant temperature of 20 DEG C, takes out and cleans;
(6) germinate and cultivate: the Taxus chinensis var. mairei Seeds processed through step (5) is placed in the culture dish that diameter is 10cm, sprouts relenting under alternate illumination condition, using 24 hours as a cycle period, cultivate 20 days.Described in the relent condition of alternate illumination be: every day first processes 12h under 25 DEG C of illumination conditions, then processes 12 hours under 15 DEG C of dark conditions.
Embodiment 2
Promote a method for Germination of Taxus mairei Seeds, comprise the steps:
(1) collection of seed: when stripping out after Taxus chinensis var. mairei Seeds maturation, selects the seed that growth is full, rinses well, naturally dry with the water of flowing;
(2) dense sulfuric acid treatment: the seed after step (1) process is soaked in the concentrated sulfuric acid, process 30s, uses running water 60min after taking out;
(3) earthing of seeds process: first wet sand is disinfected, concrete sterilization method soaks 10 minutes with the sodium hypochlorite solution that mass percentage concentration is 1%, and then with distilled water flushing extremely without chlorine taste.Then Taxus chinensis var. mairei Seeds is mixed with wet sand, after plastic bag sealing, be placed in 4 DEG C of refrigerator low temperature treatment, take out after 25d and clean;
(4) MgCl
2process: the MgCl Taxus chinensis var. mairei Seeds processed through step (3) being put into 90mg/L
220h is soaked in solution;
(5) plant catabolic enzyme process: it is in the plant catabolic enzyme solution of 8% that the seed after step (4) process is immersed in concentration, processes 20h under the constant temperature of 20 DEG C, takes out and cleans;
(6) germinate and cultivate: the Taxus chinensis var. mairei Seeds processed through step (5) is placed in the culture dish that diameter is 16cm, sprouts relenting under alternate illumination condition, using 24 hours as a cycle period, cultivate 30 days.The condition of the described alternate illumination that relents is: every day first processes 12h under 25 DEG C of illumination conditions, then processes 12 hours under 15 DEG C of dark conditions.
Embodiment 3
Promote a method for Germination of Taxus mairei Seeds, comprise the steps:
(1) collection of seed: when stripping out after Taxus chinensis var. mairei Seeds maturation, selects the seed that growth is full, rinses well, naturally dry with the water of flowing;
(2) dense sulfuric acid treatment: the seed after step (1) process is soaked in the concentrated sulfuric acid, process 20s, uses running water 40min after taking out;
(3) earthing of seeds process: first wet sand is disinfected, concrete sterilization method soaks 10 minutes with the sodium hypochlorite solution that mass percentage concentration is 1%, and then with distilled water flushing extremely without chlorine taste.Then Taxus chinensis var. mairei Seeds is mixed with wet sand, after plastic bag sealing, be placed in 4 DEG C of refrigerator low temperature treatment, take out after 22d and clean;
(4) MgCl
2process: the MgCl Taxus chinensis var. mairei Seeds processed through step (3) being put into 75mg/L
225h is soaked in solution;
(5) plant catabolic enzyme process: it is in the plant catabolic enzyme solution of 7% that the seed after step (4) process is immersed in concentration, processes 22h under the constant temperature of 20 DEG C, takes out and cleans;
(6) germinate and cultivate: the Taxus chinensis var. mairei Seeds processed through step (5) is placed in the culture dish that diameter is 12cm, sprouts relenting under alternate illumination condition, using 24 hours as a cycle period, cultivate 25 days.The condition of the described alternate illumination that relents is: every day first processes 12h under 25 DEG C of illumination conditions, then processes 12 hours under 15 DEG C of dark conditions.
Comparative example 1
The seed of southern enqlish yew is only carried out Stratificated treatment, specifically processes with the step (3) in embodiment 3.
Comparative example 2
The seed of southern enqlish yew is handled as follows, specifically with step (1) ~ (3) in embodiment 3.
Comparative example 3
The seed of southern enqlish yew is handled as follows, specifically with step (1) ~ (4) in embodiment 3.
The percentage of seedgermination of embodiment 1-3 reaches 82%, 85%, 88% respectively, and germination vigor is respectively 73.2%, 76.5%, 78.8%; And the percentage of seedgermination of comparative example 1 ~ 3 is respectively 21%, 45%, 68%, greatly improve relative to the germination rate of the seed of the southern enqlish yew comparative example after process of the present invention and germination vigor.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (3)
1. promote a method for Germination of Taxus mairei Seeds, it is characterized in that: comprise the steps:
(1) collection of seed: when stripping out after Taxus chinensis var. mairei Seeds maturation, selects the seed that growth is full, rinses well, naturally dry with the water of flowing;
(2) dense sulfuric acid treatment: the seed after step (1) process is soaked in the concentrated sulfuric acid, process 10 ~ 30s, with running water 30 ~ 60min after taking out;
(3) earthing of seeds process: mixed with wet sand by Taxus chinensis var. mairei Seeds, after plastic bag sealing, be placed in 4 DEG C of refrigerator low temperature treatment, takes out after 20 ~ 25d and cleans;
(4) MgCl
2process: the MgCl Taxus chinensis var. mairei Seeds processed through step (3) being put into 60 ~ 90mg/L
220 ~ 30h is soaked in solution;
(5) plant catabolic enzyme process: it is in the plant catabolic enzyme solution of 6 ~ 8% that the seed after step (4) process is immersed in concentration, processes 20 ~ 24h under the constant temperature of 20 DEG C, takes out and cleans;
(6) germinate and cultivate: the Taxus chinensis var. mairei Seeds processed through step (5) is placed in the culture dish that diameter is 10 ~ 16cm, sprouts relenting under alternate illumination condition, using 24 hours as a cycle period, cultivate 20 ~ 30 days.
2. the method promoting Germination of Taxus mairei Seeds as claimed in claim 1, it is characterized in that: in the earthing of seeds processing procedure described in step (3), seed is 1: 3 with the wet husky volume ratio mixed, described wet sand will through disinfecting, concrete sterilization method soaks 10 minutes with the sodium hypochlorite solution that mass percentage concentration is 1%, and then with distilled water flushing extremely without chlorine taste.
3. the method promoting Germination of Taxus mairei Seeds as claimed in claim 1, it is characterized in that: the condition of the alternate illumination that relents described in step (6) is: every day first processes 12h under 25 DEG C of illumination conditions, then process 12 hours under 15 DEG C of dark conditions.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105432380A (en) * | 2015-12-02 | 2016-03-30 | 平顶山学院 | Method for preventing and controlling taxus chinensis anthracnose |
| CN106258074A (en) * | 2016-08-13 | 2017-01-04 | 复旦大学 | A kind of method of Scirpus mariqueter seed germination |
| CN107593028A (en) * | 2017-10-23 | 2018-01-19 | 柳州雅瑞科技有限公司 | A kind of method for promoting Robinia pseudoacacia Seeds to sprout |
| CN108522120A (en) * | 2018-04-14 | 2018-09-14 | 浙江新景市政园林有限公司 | The breeding method of Chinese yew |
| CN108934268A (en) * | 2018-07-03 | 2018-12-07 | 东北林业大学 | A method of abolishing Chinese yew seed dormancy |
| CN109329026A (en) * | 2018-11-30 | 2019-02-15 | 青岛农业大学 | A kind of method that high temperature-intermittent warming obtains Sweetpotato Viruses Elimination seedling |
| CN110896701A (en) * | 2019-12-10 | 2020-03-24 | 甘肃古道昕丰农业科技有限公司 | Method for improving germination rate of taxus chinensis seeds |
| CN112789965A (en) * | 2021-01-14 | 2021-05-14 | 上饶师范学院 | Method for promoting germination of radix tetrastigme seeds |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105432380A (en) * | 2015-12-02 | 2016-03-30 | 平顶山学院 | Method for preventing and controlling taxus chinensis anthracnose |
| CN106258074A (en) * | 2016-08-13 | 2017-01-04 | 复旦大学 | A kind of method of Scirpus mariqueter seed germination |
| CN106258074B (en) * | 2016-08-13 | 2020-06-12 | 复旦大学 | Scirpus marianus seed germination method |
| CN107593028A (en) * | 2017-10-23 | 2018-01-19 | 柳州雅瑞科技有限公司 | A kind of method for promoting Robinia pseudoacacia Seeds to sprout |
| CN108522120A (en) * | 2018-04-14 | 2018-09-14 | 浙江新景市政园林有限公司 | The breeding method of Chinese yew |
| CN108934268A (en) * | 2018-07-03 | 2018-12-07 | 东北林业大学 | A method of abolishing Chinese yew seed dormancy |
| CN109329026A (en) * | 2018-11-30 | 2019-02-15 | 青岛农业大学 | A kind of method that high temperature-intermittent warming obtains Sweetpotato Viruses Elimination seedling |
| CN110896701A (en) * | 2019-12-10 | 2020-03-24 | 甘肃古道昕丰农业科技有限公司 | Method for improving germination rate of taxus chinensis seeds |
| CN112789965A (en) * | 2021-01-14 | 2021-05-14 | 上饶师范学院 | Method for promoting germination of radix tetrastigme seeds |
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