CN105004821A - 微型hplc设备 - Google Patents
微型hplc设备 Download PDFInfo
- Publication number
- CN105004821A CN105004821A CN201510312059.9A CN201510312059A CN105004821A CN 105004821 A CN105004821 A CN 105004821A CN 201510312059 A CN201510312059 A CN 201510312059A CN 105004821 A CN105004821 A CN 105004821A
- Authority
- CN
- China
- Prior art keywords
- liquid
- chromatography apparatus
- chromatographic column
- fluid
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004128 high performance liquid chromatography Methods 0.000 title abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 239000012530 fluid Substances 0.000 claims abstract description 25
- 238000004811 liquid chromatography Methods 0.000 claims abstract description 23
- 238000004891 communication Methods 0.000 claims abstract description 5
- 238000003860 storage Methods 0.000 claims description 6
- 238000004587 chromatography analysis Methods 0.000 abstract description 4
- 239000007789 gas Substances 0.000 description 34
- 238000001514 detection method Methods 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 10
- 238000000180 cavity ring-down spectroscopy Methods 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 239000012071 phase Substances 0.000 description 8
- 150000001875 compounds Chemical group 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 238000005086 pumping Methods 0.000 description 7
- 239000006096 absorbing agent Substances 0.000 description 6
- 239000003480 eluent Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000005855 radiation Effects 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000002245 particle Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 239000011358 absorbing material Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000000945 filler Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000004377 microelectronic Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000010363 phase shift Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000004224 UV/Vis absorption spectrophotometry Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000005370 electroosmosis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000005350 fused silica glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000005055 memory storage Effects 0.000 description 1
- 238000004226 microchip electrophoresis Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000008450 motivation Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012764 semi-quantitative analysis Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000004704 ultra performance liquid chromatography Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B19/00—Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B1/00 - F04B17/00
- F04B19/006—Micropumps
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/16—Injection
- G01N30/22—Injection in high pressure liquid systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/36—Control of physical parameters of the fluid carrier in high pressure liquid systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
- G01N2030/324—Control of physical parameters of the fluid carrier of pressure or speed speed, flow rate
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
- G01N2030/326—Control of physical parameters of the fluid carrier of pressure or speed pumps
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
Landscapes
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Fluid Mechanics (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1010737.3 | 2010-06-25 | ||
| GBGB1010737.3A GB201010737D0 (en) | 2010-06-25 | 2010-06-25 | Minature HPLC device |
| CN201180031388.6A CN103180726B (zh) | 2010-06-25 | 2011-06-27 | 微型hplc设备 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180031388.6A Division CN103180726B (zh) | 2010-06-25 | 2011-06-27 | 微型hplc设备 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105004821A true CN105004821A (zh) | 2015-10-28 |
Family
ID=42583011
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510312059.9A Pending CN105004821A (zh) | 2010-06-25 | 2011-06-27 | 微型hplc设备 |
| CN201180031388.6A Expired - Fee Related CN103180726B (zh) | 2010-06-25 | 2011-06-27 | 微型hplc设备 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180031388.6A Expired - Fee Related CN103180726B (zh) | 2010-06-25 | 2011-06-27 | 微型hplc设备 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9354209B2 (https=) |
| EP (1) | EP2585820A1 (https=) |
| JP (1) | JP6043715B2 (https=) |
| CN (2) | CN105004821A (https=) |
| AU (1) | AU2011268682B2 (https=) |
| BR (1) | BR112012032726A2 (https=) |
| CA (1) | CA2803962A1 (https=) |
| GB (1) | GB201010737D0 (https=) |
| WO (1) | WO2011161481A1 (https=) |
| ZA (1) | ZA201300607B (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201213537D0 (en) | 2012-07-30 | 2012-09-12 | Imp Innovations Ltd | Self-limiting injection assembly for sample introduction in HPLC |
| WO2014055331A1 (en) * | 2012-10-03 | 2014-04-10 | Waters Technologies Corporation | A chromatography column |
| CN103439309A (zh) * | 2013-08-19 | 2013-12-11 | 南通大学 | 高灵敏度的co气体光谱传感器的制备方法 |
| TWI579553B (zh) | 2013-11-24 | 2017-04-21 | 中央研究院 | 以紫外光－可見光檢測之高效能液相層析設備及方法 |
| WO2015110860A1 (en) * | 2014-01-24 | 2015-07-30 | Dh Technologies Development Pte. Ltd. | Systems and methods for delivering liquid to an ion source |
| GB2534936B (en) * | 2015-02-06 | 2021-09-08 | Agilent Technologies Inc | Liquid chromatography analysis unit |
| JP6704920B2 (ja) * | 2015-10-14 | 2020-06-03 | アルプスアルパイン株式会社 | 流路構造体および測定対象液体の測定装置 |
| US9513220B1 (en) * | 2015-12-29 | 2016-12-06 | International Business Machines Corporation | On-chip molecule fluorescence detection |
| FR3055703B1 (fr) * | 2016-09-05 | 2020-12-18 | Elichens | Procede d’analyse d’un gaz |
| US10995740B2 (en) * | 2016-09-26 | 2021-05-04 | Shimadzu Corporation | Switching valve, binary pump, and liquid chromatograph with binary pump |
| JP7335810B2 (ja) * | 2017-03-30 | 2023-08-30 | ９０８ デバイセズ インク． | 生物学的試料のマイクロ流体分析 |
| WO2019165249A1 (en) * | 2018-02-23 | 2019-08-29 | Silcotek Corp | Liquid chromatography technique |
| CN108593813B (zh) * | 2018-07-17 | 2020-04-10 | 厦门大学 | 手持式一体化液相色谱仪 |
| WO2022153571A1 (ja) * | 2021-01-15 | 2022-07-21 | エースバイオアナリシス株式会社 | 走査ユニット、システムおよび方法、ならびにマイクロカラム |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0583003A1 (en) * | 1992-08-13 | 1994-02-16 | Perseptive Biosystems, Inc. | Fluid metering, mixing and composition control system |
| US20030223913A1 (en) * | 2002-06-03 | 2003-12-04 | Nanostream, Inc. | Microfluidic separation devices and methods |
| US20040124085A1 (en) * | 2002-06-26 | 2004-07-01 | California Institute Of Technology | Microfluidic devices and methods with electrochemically actuated sample processing |
| CN1749750A (zh) * | 2004-09-13 | 2006-03-22 | 聂智明 | 高效液相色谱仪 |
| CN101206205A (zh) * | 2006-12-22 | 2008-06-25 | 中国科学院大连化学物理研究所 | 微流量液相色谱在线大体积进样的方法和专用装置 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2389891B1 (https=) * | 1977-05-02 | 1981-07-03 | Prolabo Sa | |
| US5408326A (en) * | 1993-04-28 | 1995-04-18 | Shell Oil Company | Dual-wavelength absorption detector adapted for continuous-flow detection |
| US6277257B1 (en) | 1997-06-25 | 2001-08-21 | Sandia Corporation | Electrokinetic high pressure hydraulic system |
| JPH11287791A (ja) * | 1998-04-01 | 1999-10-19 | Naohiro Soga | キャピラリーカラム |
| AU5877600A (en) | 1999-06-22 | 2001-01-09 | Agilent Technologies Inc. | Apparatus for the operation of a microfluidic device |
| US7530257B2 (en) * | 2002-09-27 | 2009-05-12 | Honeywell International Inc. | Phased micro analyzer VIII |
| EP1635174A1 (en) * | 2003-02-28 | 2006-03-15 | Japan Science and Technology Agency | Method of separating sugar from compound having glycoside bond, sugar separation system, sugar separation agent kit, standardized sample for sugar separation and assessment system |
| JP4307221B2 (ja) * | 2003-11-11 | 2009-08-05 | 株式会社日立ハイテクノロジーズ | 進捗管理方法及び装置 |
| CN1755351A (zh) * | 2004-09-30 | 2006-04-05 | 中国科学院大连化学物理研究所 | 发光二极管诱导荧光微型检测器 |
| CN100406886C (zh) * | 2005-06-22 | 2008-07-30 | 夏之宁 | 一种微型化的液相色谱分离方法 |
| JP2009508729A (ja) | 2005-09-21 | 2009-03-05 | ウオーターズ・インベストメンツ・リミテツド | 高圧チュービング |
| EP1965919A2 (en) | 2005-11-11 | 2008-09-10 | Molecular Vision Limited | Microfluidic device |
| JP2007163252A (ja) * | 2005-12-13 | 2007-06-28 | Sekisui Chem Co Ltd | 液体クロマトグラフ用測定カートリッジ及び液体クロマトグラフ装置 |
| US8268625B2 (en) | 2006-03-24 | 2012-09-18 | Arkray, Inc. | Method of measuring glycated hemoglobin concentration and concentration measuring apparatus |
| EP1999465B1 (en) | 2006-03-24 | 2016-11-16 | Waters Technologies Corporation | Ceramic-based chromatography apparatus |
| US8696597B2 (en) * | 2006-04-03 | 2014-04-15 | Nipro Diagnostics, Inc. | Diagnostic meter |
| JP4253667B2 (ja) * | 2006-06-02 | 2009-04-15 | 株式会社日立ハイテクノロジーズ | 分離分析装置 |
| CN1963497A (zh) * | 2006-11-08 | 2007-05-16 | 浙江大学 | 微分析芯片上的微液相色谱分离方法及其装置 |
| JP4946647B2 (ja) * | 2007-06-14 | 2012-06-06 | 株式会社島津製作所 | 試料前処理装置及び液体クロマトグラフ装置 |
| KR100966590B1 (ko) * | 2007-12-11 | 2010-06-29 | 한국전자통신연구원 | 생체신호 측정 장치들의 상호 협업 방법 및 시스템 |
| JP5181650B2 (ja) * | 2007-12-13 | 2013-04-10 | 株式会社島津製作所 | 分析システム |
-
2010
- 2010-06-25 GB GBGB1010737.3A patent/GB201010737D0/en not_active Ceased
-
2011
- 2011-06-27 EP EP11730056.6A patent/EP2585820A1/en not_active Withdrawn
- 2011-06-27 JP JP2013515975A patent/JP6043715B2/ja not_active Expired - Fee Related
- 2011-06-27 CA CA2803962A patent/CA2803962A1/en not_active Abandoned
- 2011-06-27 BR BR112012032726A patent/BR112012032726A2/pt not_active IP Right Cessation
- 2011-06-27 CN CN201510312059.9A patent/CN105004821A/zh active Pending
- 2011-06-27 CN CN201180031388.6A patent/CN103180726B/zh not_active Expired - Fee Related
- 2011-06-27 AU AU2011268682A patent/AU2011268682B2/en not_active Ceased
- 2011-06-27 US US13/806,233 patent/US9354209B2/en not_active Expired - Fee Related
- 2011-06-27 WO PCT/GB2011/051217 patent/WO2011161481A1/en not_active Ceased
-
2013
- 2013-01-23 ZA ZA2013/00607A patent/ZA201300607B/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0583003A1 (en) * | 1992-08-13 | 1994-02-16 | Perseptive Biosystems, Inc. | Fluid metering, mixing and composition control system |
| US20030223913A1 (en) * | 2002-06-03 | 2003-12-04 | Nanostream, Inc. | Microfluidic separation devices and methods |
| US20040124085A1 (en) * | 2002-06-26 | 2004-07-01 | California Institute Of Technology | Microfluidic devices and methods with electrochemically actuated sample processing |
| CN1749750A (zh) * | 2004-09-13 | 2006-03-22 | 聂智明 | 高效液相色谱仪 |
| CN101206205A (zh) * | 2006-12-22 | 2008-06-25 | 中国科学院大连化学物理研究所 | 微流量液相色谱在线大体积进样的方法和专用装置 |
Non-Patent Citations (1)
| Title |
|---|
| B. BAHNEV ET AL.: "Miniaturized Cavity Ring-Down Detection in a Liquid Flow Cell", 《ANALYTICAL CHEMISTRY》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| GB201010737D0 (en) | 2010-08-11 |
| CA2803962A1 (en) | 2011-12-29 |
| AU2011268682A1 (en) | 2013-01-10 |
| CN103180726A (zh) | 2013-06-26 |
| JP2013529780A (ja) | 2013-07-22 |
| ZA201300607B (en) | 2014-05-26 |
| EP2585820A1 (en) | 2013-05-01 |
| CN103180726B (zh) | 2015-07-01 |
| AU2011268682B2 (en) | 2015-01-29 |
| US9354209B2 (en) | 2016-05-31 |
| JP6043715B2 (ja) | 2016-12-14 |
| BR112012032726A2 (pt) | 2018-02-27 |
| WO2011161481A1 (en) | 2011-12-29 |
| US20130219999A1 (en) | 2013-08-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151028 |