CN105002198A - 用于培育表达人血清白蛋白的转基因猪的成套产品及其应用 - Google Patents
用于培育表达人血清白蛋白的转基因猪的成套产品及其应用 Download PDFInfo
- Publication number
- CN105002198A CN105002198A CN201510483684.XA CN201510483684A CN105002198A CN 105002198 A CN105002198 A CN 105002198A CN 201510483684 A CN201510483684 A CN 201510483684A CN 105002198 A CN105002198 A CN 105002198A
- Authority
- CN
- China
- Prior art keywords
- serum albumin
- human serum
- seq
- pig
- zygote
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091006905 Human Serum Albumin Proteins 0.000 title claims abstract description 82
- 102000008100 Human Serum Albumin Human genes 0.000 title claims abstract description 67
- 230000009261 transgenic effect Effects 0.000 title claims abstract description 42
- 238000009395 breeding Methods 0.000 title abstract 2
- 230000001488 breeding effect Effects 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 28
- 241000282898 Sus scrofa Species 0.000 claims description 42
- 108091033409 CRISPR Proteins 0.000 claims description 40
- 239000002773 nucleotide Substances 0.000 claims description 37
- 125000003729 nucleotide group Chemical group 0.000 claims description 37
- 108020004414 DNA Proteins 0.000 claims description 32
- 239000013598 vector Substances 0.000 claims description 20
- 108091027544 Subgenomic mRNA Proteins 0.000 claims description 18
- 235000018102 proteins Nutrition 0.000 claims description 16
- 102000004169 proteins and genes Human genes 0.000 claims description 16
- 239000002299 complementary DNA Substances 0.000 claims description 14
- 102000002322 Egg Proteins Human genes 0.000 claims description 13
- 108010000912 Egg Proteins Proteins 0.000 claims description 13
- 108020004999 messenger RNA Proteins 0.000 claims description 13
- 210000004681 ovum Anatomy 0.000 claims description 13
- 238000000338 in vitro Methods 0.000 claims description 12
- 108020004707 nucleic acids Proteins 0.000 claims description 12
- 102000039446 nucleic acids Human genes 0.000 claims description 12
- 150000007523 nucleic acids Chemical group 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 12
- 244000309715 mini pig Species 0.000 claims description 10
- 239000012620 biological material Substances 0.000 claims description 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 6
- 125000000539 amino acid group Chemical group 0.000 claims description 4
- 230000008034 disappearance Effects 0.000 claims description 4
- 238000009396 hybridization Methods 0.000 claims description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 3
- 230000004720 fertilization Effects 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000013459 approach Methods 0.000 abstract description 2
- 210000004602 germ cell Anatomy 0.000 abstract 1
- 241001071864 Lethrinus laticaudis Species 0.000 description 26
- 239000000047 product Substances 0.000 description 26
- 239000000243 solution Substances 0.000 description 18
- 238000013518 transcription Methods 0.000 description 13
- 230000035897 transcription Effects 0.000 description 11
- 238000010354 CRISPR gene editing Methods 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 102000009027 Albumins Human genes 0.000 description 7
- 108010088751 Albumins Proteins 0.000 description 7
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 7
- 239000012634 fragment Substances 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 101710163270 Nuclease Proteins 0.000 description 6
- 150000001413 amino acids Chemical group 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 230000008263 repair mechanism Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 101000930463 Sus scrofa Albumin Proteins 0.000 description 5
- 238000010459 TALEN Methods 0.000 description 5
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 description 5
- 108010021522 pork drug combination 30:70 pork; isophane insulin insulin Proteins 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 125000006850 spacer group Chemical group 0.000 description 5
- 230000004568 DNA-binding Effects 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000005520 cutting process Methods 0.000 description 4
- 238000010362 genome editing Methods 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 238000012797 qualification Methods 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 108020005004 Guide RNA Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 2
- 108091036066 Three prime untranslated region Proteins 0.000 description 2
- 108091028113 Trans-activating crRNA Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000011559 double-strand break repair via nonhomologous end joining Effects 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000012239 gene modification Methods 0.000 description 2
- 230000009368 gene silencing by RNA Effects 0.000 description 2
- 230000005017 genetic modification Effects 0.000 description 2
- 235000013617 genetically modified food Nutrition 0.000 description 2
- 238000002744 homologous recombination Methods 0.000 description 2
- 230000006801 homologous recombination Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 230000006780 non-homologous end joining Effects 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 210000004340 zona pellucida Anatomy 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 206010001052 Acute respiratory distress syndrome Diseases 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 206010048962 Brain oedema Diseases 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 241000252212 Danio rerio Species 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 244000148064 Enicostema verticillatum Species 0.000 description 1
- 208000034767 Hypoproteinaemia Diseases 0.000 description 1
- 206010027626 Milia Diseases 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 108091081548 Palindromic sequence Proteins 0.000 description 1
- 208000013616 Respiratory Distress Syndrome Diseases 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 101710185494 Zinc finger protein Proteins 0.000 description 1
- 102100023597 Zinc finger protein 816 Human genes 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 208000011341 adult acute respiratory distress syndrome Diseases 0.000 description 1
- 201000000028 adult respiratory distress syndrome Diseases 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 208000027119 bilirubin metabolic disease Diseases 0.000 description 1
- 208000006752 brain edema Diseases 0.000 description 1
- 208000029028 brain injury Diseases 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 101150039352 can gene Proteins 0.000 description 1
- 230000002612 cardiopulmonary effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000005782 double-strand break Effects 0.000 description 1
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000012173 estrus Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010363 gene targeting Methods 0.000 description 1
- 238000001631 haemodialysis Methods 0.000 description 1
- 230000000322 hemodialysis Effects 0.000 description 1
- 208000036796 hyperbilirubinemia Diseases 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000009437 off-target effect Effects 0.000 description 1
- 210000003101 oviduct Anatomy 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000012474 protein marker Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000011830 transgenic mouse model Methods 0.000 description 1
- 102000003390 tumor necrosis factor Human genes 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510483684.XA CN105002198B (zh) | 2015-08-07 | 2015-08-07 | 用于培育表达人血清白蛋白的转基因猪的成套产品及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510483684.XA CN105002198B (zh) | 2015-08-07 | 2015-08-07 | 用于培育表达人血清白蛋白的转基因猪的成套产品及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105002198A true CN105002198A (zh) | 2015-10-28 |
CN105002198B CN105002198B (zh) | 2018-07-06 |
Family
ID=54375062
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510483684.XA Active CN105002198B (zh) | 2015-08-07 | 2015-08-07 | 用于培育表达人血清白蛋白的转基因猪的成套产品及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105002198B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107641155A (zh) * | 2016-07-20 | 2018-01-30 | 北京泰柏生物科技有限责任公司 | 一种在植物中表达重组人血清白蛋白的方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140186843A1 (en) * | 2012-12-12 | 2014-07-03 | Massachusetts Institute Of Technology | Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
WO2015006747A2 (en) * | 2013-07-11 | 2015-01-15 | Moderna Therapeutics, Inc. | Compositions comprising synthetic polynucleotides encoding crispr related proteins and synthetic sgrnas and methods of use. |
CN104531685A (zh) * | 2014-11-27 | 2015-04-22 | 中国农业科学院北京畜牧兽医研究所 | 特异识别猪H11位点的sgRNA及其编码DNA和应用 |
CN104531686A (zh) * | 2014-11-27 | 2015-04-22 | 中国农业科学院北京畜牧兽医研究所 | 一种利用定点切割系统对猪h11位点定点插入的方法 |
CN104593422A (zh) * | 2015-01-08 | 2015-05-06 | 中国农业大学 | 一种抗蓝耳病克隆猪的制备方法 |
WO2015070083A1 (en) * | 2013-11-07 | 2015-05-14 | Editas Medicine,Inc. | CRISPR-RELATED METHODS AND COMPOSITIONS WITH GOVERNING gRNAS |
-
2015
- 2015-08-07 CN CN201510483684.XA patent/CN105002198B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140186843A1 (en) * | 2012-12-12 | 2014-07-03 | Massachusetts Institute Of Technology | Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
WO2015006747A2 (en) * | 2013-07-11 | 2015-01-15 | Moderna Therapeutics, Inc. | Compositions comprising synthetic polynucleotides encoding crispr related proteins and synthetic sgrnas and methods of use. |
WO2015070083A1 (en) * | 2013-11-07 | 2015-05-14 | Editas Medicine,Inc. | CRISPR-RELATED METHODS AND COMPOSITIONS WITH GOVERNING gRNAS |
CN104531685A (zh) * | 2014-11-27 | 2015-04-22 | 中国农业科学院北京畜牧兽医研究所 | 特异识别猪H11位点的sgRNA及其编码DNA和应用 |
CN104531686A (zh) * | 2014-11-27 | 2015-04-22 | 中国农业科学院北京畜牧兽医研究所 | 一种利用定点切割系统对猪h11位点定点插入的方法 |
CN104593422A (zh) * | 2015-01-08 | 2015-05-06 | 中国农业大学 | 一种抗蓝耳病克隆猪的制备方法 |
Non-Patent Citations (4)
Title |
---|
BIN SHEN等: "Generation of gene-modified mice via Cas9/RNA-mediated gene targeting", 《CELL RESEARCH》 * |
NCBI: "Homo sapiens albumin (ALB), mRNA", 《GENBANK》 * |
SUN, S.等: "Sus scrofa albumin gene, complete cds", 《GENBANK》 * |
牟玉莲等: "猪规模化转基因技术体系构建及其应用", 《中国农业科学》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107641155A (zh) * | 2016-07-20 | 2018-01-30 | 北京泰柏生物科技有限责任公司 | 一种在植物中表达重组人血清白蛋白的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN105002198B (zh) | 2018-07-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
GB2578026A (en) | Method for constructing GLRX1 gene knock-out animal model based on CRISPR/CAS9 | |
CN108949824A (zh) | 基于HMEJ的方法介导Ipr1定点插入获取转基因牛胎儿成纤维细胞的方法 | |
CN104531705A (zh) | 利用CRISPR-Cas9系统敲除动物myostatin基因的方法 | |
CN108165581B (zh) | 采用单链核苷酸片段体外修复hba2基因突变的方法 | |
CN104531704A (zh) | 利用CRISPR-Cas9系统敲除动物FGF5基因的方法 | |
CN111979264B (zh) | 基于CRISPR/Cas9系统对博落回PDS基因编辑体系的构建方法及应用 | |
JP6958917B2 (ja) | 遺伝子ノックイン細胞の作製方法 | |
CN106119284A (zh) | 一种用于构建免疫缺陷动物模型的产品及其应用 | |
CN106086031B (zh) | 猪肌抑素基因编辑位点及其应用 | |
CN111575319B (zh) | 一种高效的crispr rnp和供体dna共位介导的基因插入或替换方法及其应用 | |
CN106520824A (zh) | 多靶点编辑系统及其用途 | |
CN109706148A (zh) | 一种用于敲除BCL11A基因或者BCL11A基因增强子的gRNA、gRNA组合物以及电转方法 | |
CN111718933B (zh) | 一种rrbp1基因敲除热带爪蛙模型的制备方法与应用 | |
CN112824529B (zh) | 一种条件性基因敲除或者拯救方法 | |
CN106754949B (zh) | 猪肌抑素基因编辑位点864-883及其应用 | |
CN111961679B (zh) | 博落回八氢番茄红素脱氢酶基因的核苷酸序列及应用 | |
CN109628493B (zh) | 一种用于制备可异体移植t细胞的基因编辑系统 | |
CN105002198A (zh) | 用于培育表达人血清白蛋白的转基因猪的成套产品及其应用 | |
CN104962595B (zh) | 一种可用于胚胎注射制备敲除小鼠的Cas9蛋白的制备方法 | |
CN109055379B (zh) | 一种转基因鸡输卵管生物反应器的制备方法 | |
CN104726490B (zh) | 表达人血清白蛋白的靶向载体及其构建方法 | |
WO2011091562A1 (zh) | 一种培育猪生长激素表达量增强的转基因动物的方法 | |
CN107058324A (zh) | 水稻根特异表达启动子POsRO4及相应水稻培育方法 | |
Muzaffar et al. | History of biotechnology | |
Kuk et al. | Rapid and efficient BAC recombineering: gain & loss screening system |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhang Pumin Inventor after: Peng Jin Inventor before: Zhang Pumin Inventor before: Peng Jin Inventor before: Bian Guangxing |
|
COR | Change of bibliographic data | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20200624 Address after: No.18-d800, Jianshe Road, Kaixuan street, Liangxiang, Fangshan District, Beijing Patentee after: Beijing ace Biotechnology Co.,Ltd. Address before: 102206 building 33, No. 1, life Garden Road, Beijing, Changping District Patentee before: BEIJING PROTEOME RESEARCH CENTER |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230329 Address after: Room 1101 and 1102, Building 9, Hexiang Technology Center, Qiantang New District, Hangzhou City, Zhejiang Province, 311119 Patentee after: Hangzhou Puji Yuancheng Biomedical Technology Co.,Ltd. Address before: No.18-D800 Jianshe Road, Kaixuan Street, Liangxiang, Fangshan District, Beijing, 102401 Patentee before: Beijing ace Biotechnology Co.,Ltd. |
|
TR01 | Transfer of patent right |