CN104983891A - Traditional Chinese medicine prescription for treating wound and preparation method of traditional Chinese medicine oil dressing based on traditional Chinese medicine prescription - Google Patents
Traditional Chinese medicine prescription for treating wound and preparation method of traditional Chinese medicine oil dressing based on traditional Chinese medicine prescription Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine prescription for treating a wound and a preparation method of traditional Chinese medicine oil dressing based on the traditional Chinese medicine prescription, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine prescription consists of 10 to 30 parts by mass of a component A and 3 to 10 parts by mass of a component B, wherein the component A is formed by the compatibility of the following raw materials in parts by mass: 15 to 35 parts of garden burnet root, 10 to 40 parts of dahurian angelica root, 5 to 30 parts of rhubarb, 15 to 40 parts of giant knotweed, 10 to 50 parts of Chinese angelica, 15 to 35 parts of dried rehmannia root, 10 to 45 parts of coptis root, 15 to 35 parts of amur corktree bark and 20 to 35 parts of baikal skullcap root; the component B is formed by the compatibility of the following raw materials in parts by mass: 5 to 15 parts of sinkiang arnebia root, 2 to 5 parts of borneol and 3 to 10 parts of bee wax. By using pure natural traditional Chinese medicinal materials as the raw materials, the traditional Chinese medicine prescription is remarkable in treatment effect, little in toxic and side effects, low in cost and safe to use; the preparation method of the traditional Chinese medicine oil dressing based on the traditional Chinese medicine prescription is simple and easy to operate.
Description
Technical field
The invention belongs to technical field of Chinese medicines, be specifically related to a kind of preparation method being used for the treatment of the Chinese medicinal formulae of wound and the Chinese medicine oil dressing based on this Chinese medicinal formulae.
Background technology
1962, British royal medical association doctor Winter confirmed in zoopery---wound is under the environment that appropriateness is moistening, and cell regeneration ability and speed of vacillating, it restores speed than more than fast again under the environment of bone dry.
Through clinical research and the practice of more than 40 years, moist wounds healing theory was accepted by American-European countries's medical profession.In China, because clinical Wound healing and bone regeneration, nursing are started late, still limitation is compared for the treatment of wound and the change scope of NM, in August, 2000, FDA (Food and Drug Adminstration) (FDA) points out in the new industry guide promulgated: maintenance wound surface moist environment is the wound processing method of standard.
Along with the continuous change of modern society people living environment, diet, pressure ulcer, dermatosis, diabetes number, various operation number are in rising trend.At present, the dressing majority used clinically still rests on application yellow sliver (ethacridine solution) cleaning and sterilizing external application, also has some Imported liquid dressing, but because of composition single, the shortcoming such as expensive, makes it apply and is restricted.At present, very lack and there is antibacterial, antiinflammatory, removing the necrotic tissue and promoting granulation, meet the liquid dressing of the various effects of wet union theory.
Summary of the invention
In order to overcome the defect that above-mentioned prior art exists, the object of the present invention is to provide a kind of preparation method being used for the treatment of the Chinese medicinal formulae of wound and the Chinese medicine oil dressing based on this Chinese medicinal formulae, this Chinese medicinal formulae with natural Chinese medicines material for raw material, evident in efficacy, toxic and side effects is little, with low cost, use safety; Preparation method based on the Chinese medicine oil dressing of this Chinese medicinal formulae is simple, easy to operate.
The present invention is achieved through the following technical solutions:
The invention discloses a kind of Chinese medicinal formulae being used for the treatment of wound, be made up of the component A of 10 ~ 30 mass parts and the B component compatibility of 3 ~ 10 mass parts;
Described component A, in mass fraction, be made up of following material combination: Radix Sanguisorbae 15 ~ 35 parts, the Radix Angelicae Dahuricae 10 ~ 40 parts, Radix Et Rhizoma Rhei 5 ~ 30 parts, Rhizoma Polygoni Cuspidati 15 ~ 40 parts, Radix Angelicae Sinensis 10 ~ 50 parts, Radix Rehmanniae 15 ~ 35 parts, Rhizoma Coptidis 10 ~ 45 parts, Cortex Phellodendri 15 ~ 35 parts, Radix Scutellariae 20 ~ 35 parts;
Described B component, in mass fraction, is made up of following material combination: Radix Arnebiae (Radix Lithospermi) 5 ~ 15 parts, Borneolum Syntheticum 2 ~ 5 parts, 3 ~ 10 parts, Cera Flava.
Preferably, this is used for the treatment of the Chinese medicinal formulae of wound, is made up of the component A of 10 ~ 30 mass parts and the B component of 3 ~ 10 mass parts;
Described component A, in mass fraction, is made up of following material combination: Radix Sanguisorbae 30 parts, the Radix Angelicae Dahuricae 30 parts, Radix Et Rhizoma Rhei 30 parts, Rhizoma Polygoni Cuspidati 30 parts, Radix Angelicae Sinensis 30 parts, Radix Rehmanniae 30 parts, Rhizoma Coptidis 30 parts, Cortex Phellodendri 30 parts, Radix Scutellariae 30 parts;
Described B component, in mass fraction, is made up of following material combination: Radix Arnebiae (Radix Lithospermi) 10 parts, Borneolum Syntheticum 3 parts, 5 parts, Cera Flava.
The invention also discloses the method adopting the above-mentioned Chinese medicinal formulae being used for the treatment of wound to prepare Chinese medicine oil dressing, comprise the following steps:
1) by prescription proportioning, pulverize after each for component A taste medicine mixing, then according to the solid-liquid ratio of 1g:3 ~ 5mL, the component A medicated powder after pulverizing is added in vegetable oil, stir, at 70 ~ 80 DEG C, process 24 ~ 48h, elimination medicinal residues, obtained component A medicine oil;
2) by prescription proportioning, pulverize after each for B component taste medicine mixing, then according to the solid-liquid ratio of 1g:30 ~ 50mL, the B component medicated powder after pulverizing is added in vegetable oil, stir, at 80 ~ 100 DEG C, process 24 ~ 48h, elimination medicinal residues, obtained B component medicine oil;
3) after component A medicine oil and B component medicine oil being pressed the mass ratio mixing of 10:1, obtained Chinese medicine oil dressing.
Compared with prior art, the present invention has following useful technique effect:
The Chinese medicinal formulae being used for the treatment of wound disclosed by the invention, formula forms by monarch principle: component A is monarch drug, and B component is ministerial drug, adjuvant drug and makes medicine, and the combination of AB plays its multiple efficacies.Radix Scutellariae in component A, Rhizoma Coptidis and Cortex Phellodendri all belong to bitter in the mouth, heat clearing away cold in nature, pathogenic fire reducing, dampness class medicine.Radix Sanguisorbae, Radix Et Rhizoma Rhei, Rhizoma Polygoni Cuspidati have antiviral, antibacterial, oxidation and removing free radicals, antitoxin antiinflammatory, the effects such as surely logical, the clearing away heat-damp and promoting diuresis of invigorating blood circulation, removing toxic substances, preventing phlegm from forming and stopping coughing.Cure mainly amenorrhea, rheumatic arthralgia, jaundice due to damp-heat, stranguria with turbid discharge leukorrhagia, burn due to hot liquid or fire, sore and toxic, venom.Radix Angelicae Sinensis, Radix Rehmanniae have treatment and lose blood puerperal, effect of hepatoasthenia hypochondrium pain.It is warm in nature for the Radix Angelicae Dahuricae, and gas fragrance, acrid in the mouth, micro-hardship, return stomach, colon, lung meridian, has the effects such as the dehumidifying of loose wind, sensible analgesia, detumescence and apocenosis.The card such as be clinically mainly used in treatment headache due to common cold, supraorbital bone pain, nasal obstruction, nasal sinusitis, toothache, leucorrhea and skin infection swell and ache.Containing acetylshikonin, Radix Arnebiae (Radix Lithospermi) essence and alkalization shikonin in Radix Arnebiae (Radix Lithospermi) in B component, there is astriction.All there is inhibitory action to staphylococcus aureus, escherichia coli, dermatophytes etc., and epithelial growth can be accelerated, promote wound healing and be applied to infected wound and neurodermatitis.Borneolum Syntheticum is that conventional Chinese medicine has multiple efficacies, can be used for the various diseases such as treatment aphtha, oral ulcer, facial acne, burn and scald, herpes zoster, skin ulcer, swelling and pain of hemorrhoid, Borneolum Syntheticum has the effect of consciousness-restoring and orifice-opening, clearing away heat to alleviate pain, erosion prevention and muscle growing, can treat various disease conditions in clinical middle compatibility application.The medical value of Cera Flava is also very high, and Chinese medicine beeswax has effect of removing toxic substances, granulation promoting, analgesic therapy, and for oral administration and external application can cure mainly has to go to the toilet pained, and dysentery pus and blood, incessant chronic diarrhea, vaginal bleeding due to excessive movement of fetus, carbuncle sore inside fire attack, bursts for a long time and do not hold back, burn due to hot liquid or fire etc.In prescription of the present invention, the effect of above each medicine, is confirmed in the Preliminay clinical trials of the medicine oil prepared by the present invention, also proves reasonability and the effectiveness of this prescription simultaneously.The advantage of this prescription display treating both the principal and the secondary aspects of a disease at the same time, absorbs the method for thinking of modern new drug research theory, solves science and the normative standard of tcm clinical practice medication, breaks through the single-mode using clinical experience accumulation as development new drug.
The invention also discloses the method preparing Chinese medicine oil dressing based on above-mentioned prescription, this preparation method is simple, and easy to operate, safety is high.The liposoluble constituent in medicine fully can be extracted by the method, and then play effect of compound medicine, illustrate that this Chinese medicine oil wound dressing preparation technology (pulverizing constant temperature to extract) is obviously better than traditional decoction pieces and decocts extraction process by efficient Liquid Detection content of berberine hydrochloride.
Accompanying drawing explanation
Fig. 1 have detected the content collection of illustrative plates of berberine hydrochloride in the medicine oil prepared by two kinds of preparation methoies for high-efficient liquid phase technique, wherein, a () is standard substance chromatogram, (b) decocts the chromatogram of berberine hydrochloride in the medicine oil that decoction pieces method obtains for traditional method; C () obtains the chromatogram of berberine hydrochloride in medicine oil for the inventive method.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Embodiment 1
Be used for the treatment of a Chinese medicinal formulae for wound, be made up of the component A of 10 mass parts and the B component compatibility of 3 mass parts;
Described component A, in mass fraction, is made up of following material combination: Radix Sanguisorbae 30 parts, the Radix Angelicae Dahuricae 30 parts, Radix Et Rhizoma Rhei 30 parts, Rhizoma Polygoni Cuspidati 30 parts, Radix Angelicae Sinensis 30 parts, Radix Rehmanniae 30 parts, Rhizoma Coptidis 30 parts, Cortex Phellodendri 30 parts, Radix Scutellariae 30 parts;
Described B component, in mass fraction, is made up of following material combination: Radix Arnebiae (Radix Lithospermi) 10 parts, Borneolum Syntheticum 3 parts, 5 parts, Cera Flava.
Adopt above-mentioned Chinese medicinal formulae to prepare the method for Chinese medicine oil dressing, comprise the following steps:
1) by prescription proportioning, pulverize after each for component A taste medicine mixing, add in 1000mL vegetable oil, stir, under in 70 DEG C of calorstats, process 48h, extract the fatty contents in Chinese medicine ingredients, then elimination medicinal residues, obtained component A medicine oil;
2) by prescription proportioning, pulverize after each for B component taste medicine mixing, add in 1000mL vegetable oil, stir, under 100 DEG C of calorstats, process 24h, elimination medicinal residues, obtained B component medicine oil;
After component A medicine oil and B component medicine oil being pressed the mass ratio mixing of 10:1, obtained Chinese medicine oil dressing.
Embodiment 2
Be used for the treatment of a Chinese medicinal formulae for wound, be made up of the component A of 10 mass parts and the B component compatibility of 10 mass parts;
Described component A, in mass fraction, is made up of following material combination: Radix Sanguisorbae 15 parts, the Radix Angelicae Dahuricae 20 parts, Radix Et Rhizoma Rhei 5 parts, Rhizoma Polygoni Cuspidati 15 parts, Radix Angelicae Sinensis 25 parts, Radix Rehmanniae 15 parts, Rhizoma Coptidis 45 parts, Cortex Phellodendri 15 parts, Radix Scutellariae 35 parts;
Described B component, in mass fraction, is made up of following material combination: Radix Arnebiae (Radix Lithospermi) 5 parts, Borneolum Syntheticum 2 parts, 3 parts, Cera Flava.
Adopt above-mentioned Chinese medicinal formulae to prepare the method for Chinese medicine oil dressing, comprise the following steps:
1) by prescription proportioning, pulverize after each for component A taste medicine mixing, add in 1000mL vegetable oil, stir, under in 80 DEG C of calorstats, process 24h, extract the fatty contents in Chinese medicine ingredients, then elimination medicinal residues, obtained component A medicine oil;
2) by prescription proportioning, pulverize after each for B component taste medicine mixing, add in 1000mL vegetable oil, stir, under 100 DEG C of calorstats, process 24h, elimination medicinal residues, obtained B component medicine oil;
After component A medicine oil and B component medicine oil being pressed the mass ratio mixing of 10:1, obtained Chinese medicine oil dressing.
For the ease of using, Chinese medicine oil dressing being processed into oil gauze further, can be used as wound external application, also can be used as deep wound filling, as the filling of renal transplantation wound, various sinus tract.
Pour into after making Chinese medicine oil dressing as stated above in rustless steel container and be heated to 130 DEG C, the sterile gauze block being cut into 10 × 20cm is put into and is boiled 5-10 minute, pulls oily yarn out subpackage and namely make Chinese medicine oil gauze after cooling.
Embodiment 3
Be used for the treatment of a Chinese medicinal formulae for wound, be made up of the component A of 15 mass parts and the B component compatibility of 3 mass parts;
Described component A, in mass fraction, is made up of following material combination: Radix Sanguisorbae 35 parts, the Radix Angelicae Dahuricae 40 parts, Radix Et Rhizoma Rhei 30 parts, Rhizoma Polygoni Cuspidati 20 parts, Radix Angelicae Sinensis 10 parts, Radix Rehmanniae 35 parts, Rhizoma Coptidis 10 parts, Cortex Phellodendri 35 parts, Radix Scutellariae 20 parts;
Described B component, in mass fraction, is made up of following material combination: Radix Arnebiae (Radix Lithospermi) 15 parts, Borneolum Syntheticum 5 parts, 10 parts, Cera Flava.
Embodiment 4
Be used for the treatment of a Chinese medicinal formulae for wound, be made up of the component A of 30 mass parts and the B component compatibility of 10 mass parts;
Described component A, in mass fraction, is made up of following material combination: Radix Sanguisorbae 20 parts, the Radix Angelicae Dahuricae 10 parts, Radix Et Rhizoma Rhei 20 parts, Rhizoma Polygoni Cuspidati 40 parts, Radix Angelicae Sinensis 50 parts, Radix Rehmanniae 20 parts, Rhizoma Coptidis 45 parts, Cortex Phellodendri 20 parts, Radix Scutellariae 35 parts;
Described B component, in mass fraction, is made up of following material combination: Radix Arnebiae (Radix Lithospermi) 10 parts, Borneolum Syntheticum 4 parts, 6 parts, Cera Flava.
Embodiment 5
Be used for the treatment of a Chinese medicinal formulae for wound, be made up of the component A of 20 mass parts and the B component compatibility of 8 mass parts;
Described component A, in mass fraction, is made up of following material combination: Radix Sanguisorbae 15 parts, the Radix Angelicae Dahuricae 20 parts, Radix Et Rhizoma Rhei 30 parts, Rhizoma Polygoni Cuspidati 20 parts, Radix Angelicae Sinensis 15 parts, Radix Rehmanniae 15 parts, Rhizoma Coptidis 10 parts, Cortex Phellodendri 15 parts, Radix Scutellariae 20 parts;
Described B component, in mass fraction, is made up of following material combination: Radix Arnebiae (Radix Lithospermi) 15 parts, Borneolum Syntheticum 2 parts, 3 parts, Cera Flava.
In order to prove the reliability of Chinese medicine oil dressing provided by the invention and preparation method thereof, the content of berberine hydrochloride in adopt high-efficient liquid phase technique to have detected respectively medicine oil that the inventive method and traditional decoction decoction pieces send out prepared prepared by method.Result is standard substance chromatogram see (a) in Fig. 1, figure, and (b) decocts the chromatogram of berberine hydrochloride in the medicine oil that decoction pieces method obtains for traditional method; C () obtains the chromatogram of berberine hydrochloride in medicine oil for the inventive method.As can be seen from the figure, through the comparison with standard substance, in the medicine oil that traditional method obtains, the concentration of berberine hydrochloride is 0.53 μ g/mL, and in the medicine oil that the inventive method obtains, the concentration of berberine hydrochloride is 6.32 μ g/mL, describes the inventive method and is better than traditional method.
1, safety experiment---Mouse Acute Toxicity is tested
Test adopts ICR mouse stomach to give Chinese medicine oil wound dressing, because all medicines are nontoxic or low cytotoxic drug in this medicine oil prescription, so acute toxicity test we have employed maximum tolerated dose method, select healthy ICR mice 20 (Xi'an hands over large medical college Experimental Animal Center to provide), male and female half and half, body weight 18 ± 2g, before test, mice fasting can't help water 12 hours, Chinese medicine oil wound dressing is given by a 0.4ml/10g gavage, cage group support is divided mice by sex after gavage, give solids mixing feedstuff, drink pure water of freely ingesting, Continuous Observation one week.Animal feeding environment, hands over large medical college Experimental Animal Center standart animal test room.Result mice daily dose is 0.4X2ml/20g, i.e. 40ml/kg, and in 7 days, mice is without death, without any toxic reaction symptom.Wherein there is loose stool in second day in 2 mices, and it is relevant that this and gavage give oil preparation, within 3rd, recovers normal.This dosage of mice is equivalent to people's consumption 480ml/kg.
2, to the stimulation of Cavia porcellus intact skin
Healthy guinea pig 10 (Xi'an hands over large medical college Experimental Animal Center to provide) is selected in this test, and male and female half and half, body weight 200 ± 20g, cut off back left and right sides hair, area is about 8cm
2normal saline is smeared in contrast on the left of every back part of animal, the Chinese medicine oil wound dressing that the present invention obtains is smeared on right side, adopt own control observational technique, smear 7 continuously, stop observation post administration 7, found that on the right side of smearing period and stopping smearing one week every Cavia porcellus, skin is the same with left side, without any skin wound repair (without red, swollen, without erythra, festering).This Chinese medicine oil wound dressing is described to normal skin without any stimulation.
3, to the Healing of rabbit skin soup wound
Object: the Chinese medicine oil wound dressing that observation the present invention obtains is to the Healing of hindering rabbit skin beauty water soup
Healthy new zealand rabbit 18 (Xi'an hands over large medical college Experimental Animal Center to provide) is selected in test, about body weight 2kg, each 9 of male and female, after urethane with 20% presses 2ml/kg auricular vein injection light anaesthesia, the sodium sulfide with 10% sloughs back part of animal spinal column diamond wool area about 10 × 10cm
2, with the simple and easy high temperature scalding device of self-control, (with reference to model Cai Bin etc., setting up of dark II degree rabbit scalding model is one civilian, Fuzhou General Hospital of Nanjing Military Command), the iron sheet barrel filling Oleum Ricini is placed on electric furnace and burns to 150 DEG C, by being placed on 10 seconds on the right side of a rabbit back bottom iron sheet barrel, can cause deepⅱ°burn model, left side is as normal control, after every rabbit is caused scalding model, be divided into 3 groups at random, i.e. saline control group, compound recipe Folium paulowniae burn line of oils, Chinese medicine oil wound dressing group.The Healing that test adopts own control observation Chinese medicine oil wound dressing to hinder rabbit skin beauty water soup.
Normal saline, compound recipe Folium paulowniae burn oil and Chinese medicine oil wound dressing is smeared respectively for 3 groups to right side.Every day 1 time, smear the healing state observed 3 treated animals and scald for 3 weeks continuously.
Result is see table 1.Wound healing situation is observed: treatment group case all healed, scar hyperplasia is not obvious.Chinese medicine oil wound dressing obviously can promote the healing of rabbit deepⅱ°burn, the shortening healing time of significantly scalding.
Table 1 wound healing effect contrast result
| Group | Number of animals | Healing time (d) | Agglutination skin histology shows |
| Normal saline group | 6 | 21.9±3.59 | There is swelling, ooze out, have infection, fester, form a scab, have cicatrix |
| Compound recipe Folium paulowniae burn oil | 6 | 14.5±4.32 ★★ | There is swelling, ooze out less, nothing is festered, infect, nothing forms a scab, cicatrix is less |
| Chinese medicine oil wound dressing | 6 | 9.56±3.12 ★★ | Swelling is light, ooze out seldom, without festering, infects, without forming a scab, without cicatrix |
Note: compare ★ ★ p ﹤ 0.01 with normal saline group
4, to the Healing of the damaging skin of rabbit
Object: observe Chinese medicine oil wound dressing to the Healing of rabbit injured skin
Model 1: healthy new zealand rabbit 30 (Xi'an hands over large medical college Experimental Animal Center to provide) is selected in trauma model test, about body weight 2kg, each 15 of male and female, after urethane with 20% presses 2ml/kg auricular vein injection light anaesthesia, sodium sulfide with 10% sloughs back part of animal spinal column diamond wool, to use area 2.45cm after iodine disinfection skin
2steel card punch is in each 2cm place, rabbit back spinal column both sides, cut 5 circular wound surface from top to bottom, distance between borehole 2.5cm, be deep to subcutaneous, (method reference Chinese J Pharmacol Toxicol, Tang Tian etc., " emodin is to the promoting healing Function and its mechanisms of rabbit experiment skin trauma " in April, 2006 for subsequent use after hemostasis, 20 (2), 112-119); By the model random packet prepared, 30 rabbit are divided at random 5 and organize greatly often group 6: be respectively blank group, pure plant line of oils, positive controls (compound recipe Folium paulowniae burn line of oils), the large small dose group for the treatment of group (Chinese medicine oil wound dressing group), left side is as normal control.Simple traumatized animals successive administration 7d.
Model 2: traumatic infection model test selects healthy new zealand rabbit 30 (Xi'an hands over large medical college Experimental Animal Center to provide), about body weight 2kg, each 15 of male and female, namely cutting is engraved in wound surface and drips staphylococcus aureus or bacillus pyocyaneus 0.1ml after causing injury, drip 1 time, concentration is respectively 1 × 10
11l
-1with 5 × 10
10l
-1.After 24h there is red, swollen and purulent secretion in animal wound, isolates staphylococcus aureus or bacillus pyocyaneus, show that infective wound surface is successfully prepared from wound tissue.After model is successfully prepared, every large group is divided into 5 groups, often organizes 6 rabbit, every rabbit 5 wound surface.Blank group, not coating; Pure plant line of oils; Positive control is all coated with compound recipe Folium paulowniae burn oil, Chinese medicine oil wound dressing group, is coated with Chinese medicine oil wound dressing once and twice.Wound adds that to drip same day of bacterial treatment be d0, d1 administration next day, every day coating 1 time, heavy dose of group coating every day 2 times.Bacterial infections successive administration 14d, covers antiseptic gauze after coating, immobilization with adhesive tape.On the left of the coating of right side in contrast, test adopts own control to observe Chinese medicine oil wound dressing to the Healing of rabbit injured skin.
Therapeutic Method
Each treated animal smears normal saline, pure vegetable oil, compound recipe Folium paulowniae burn oil and the large small dose group of Chinese medicine oil wound dressing to right side respectively.Every day 1 time, heavy dose of group every day 2 times, smear the healing state of 2 weeks each treated animal wounds of observation continuously.
Result:
Table 2 Chinese medicine oil wound dressing is to the Healing of rabbit injured skin
| Group | Number of animals | Healing time (d) | Agglutination skin histology shows |
| Normal saline group | 6 | 21.9±3.59 | There is swelling, ooze out, have infection, fester, form a scab, have cicatrix |
| Compound recipe Folium paulowniae burn oil | 6 | 14.5±4.32 ★★ | There is swelling, ooze out less, nothing is festered, infect, nothing forms a scab, cicatrix is less |
| The heavy dose of group of Chinese medicine oil wound dressing | 6 | 9.56±3.12 ★★ | Swelling is light, ooze out seldom, without festering, infects, without forming a scab, without cicatrix |
| Chinese medicine oil wound dressing small dose group | 6 | 11.9±3.06 ★★ | Swelling is light, ooze out seldom, without festering, infects, without forming a scab, without cicatrix |
| Pure plant line of oils | 6 | 18.6±4.18 | There is swelling, ooze out less, have and fester, infect, without incrustation, without cicatrix |
Note: compare ★ ★ p ﹤ 0.01 with normal saline group
Table 3 Chinese medicine oil wound dressing is to the Healing of the infectious injured skin of rabbit
| Group | Number of animals | Healing time (d) | Agglutination skin histology shows |
| Normal saline group | 6 | 25.4±3.59 | There is swelling, ooze out, have infection, suppurate, fester, form a scab, have cicatrix |
| Compound recipe Folium paulowniae burn oil | 6 | 16.9±3.44 ★★ | There is swelling, ooze out less, fester, suppurate, have infection, nothing forms a scab, cicatrix is less |
| The heavy dose of group of Chinese medicine oil wound dressing | 6 | 11.25±3.36 ★★ | Swelling is light, ooze out seldom, without infection of festering, have, without incrustation, without cicatrix |
| Chinese medicine oil wound dressing small dose group | 6 | 13.1±3.22 ★★ | There is swelling, ooze out seldom, without infection of festering, have, without incrustation, without cicatrix |
| Pure plant line of oils | 6 | 20.6±4.34 | Have swelling, ooze out less, have fester, have infection, without incrustation, without cicatrix |
Note: compare ★ ★ p ﹤ 0.01 with normal saline group
Can find out that Chinese medicine oil wound dressing obviously can shorten the damaging skin of rabbit and infect the healing time of injured skin from table 2, table 3, more all have significant difference (p ﹤ 0.01) with matched group.And wound can be reduced ooze out, suppress the growth of infected wound staphylococcus aureus, pus bacillus, infection control, promote wound healing, and without cicatrix.
5, Chinese medicine oil wound dressing is on the impact of rat gastric ulcer caused by argon-gas knife
Adopt healthy rat 50, the rat of body weight 200 ± 20g, male and female dual-purpose.Be divided into 5 groups at random, often organize 10.Pre-operative anxiety can't help water 12 hours, with 20% urethane anesthesia, lies on the back and is fixed on operating-table, cropping preserved skin, under xiphoid-process, cut abdominal cavity open be about 2cm, clamp out stomach gently, cut an osculum at greater gastric curvature place and be about 1cm, clean gastric content, from wound, stomach is opened exposure gastric mucosa gently, with argon-gas knife calcination stomach bottom, light pedal switch 3 times, persistent period is about 3-5 second, and calcination area is about 0.5cm
2, burn to gastric mucosa and turn white, turn black as degree, and can smell burn paste taste, then use catgut suture coat of the stomach, then use silk suture muscle and skin, with iodophor disinfection skin pneumoretroperitoneum injection penicillin infection.
Modeling starts treatment after one week, the animal survived by Post operation is divided into 5 groups (except Normal groups) at random, and Normal group gavage gives to wait capacity normal saline (0.4ml/100g); Positive group gives hydrotalcite tablet suspension 0.4ml/100g by 0.4g/kg; Model group gives to wait capacity normal saline; Chinese medicine oil wound dressing heavy dose group gavage gives 2l/kg, and middle dosage group gavage gives 1l/kg, and small dose group gavage gives 0.5kg.Treatment surrounding post processing animal, fasting 12 hours before process.
Animal is opened abdominal cavity with after 20% urethane anesthesia, and ventral aorta is drawn blood, and gets 1ml and does routine blood test detection, get 5ml centrifugal blood and get serum, place-24 DEG C of refrigerator freezings and preserve.Get ulcer spot gastric tissue one fritter and do pathological examination, after separately getting a fritter homogenate, centrifuging and taking supernatant is placed-24 DEG C of refrigerator freezings and is preserved the bFGF detection being tissue fluid Elisa.
Result of the test opens stomach visible model group animal calcination position coat of the stomach to be had obvious depression, out-of-flatness, has hyperemia, bleeding, none example perforation.Positive controls and the new treatment group of rehabilitation are clearly better, and ulcer surface reduces, and none example is hemorrhage and red and swollen, ulcer surface is almost smooth.Design parameter result is see table 4,5.
Table 4 Chinese medicine oil wound dressing is on the routine blood test impact of Gastric Ulcer Rats caused by argon-gas knife
Table 4 is visible, and test each group and matched group and compare leukocyte and raise obviously, model group and middle dosage group have compared significant difference (P < 0.01) with matched group; Erythrocyte, platelet all have rising trend, model success is described, and does not almost affect lymphocyte.
Table 5 Chinese medicine oil wound dressing is on the impact (BFGF) of rat gastric ulcer caused by argon-gas knife
As can be seen from Table 5, rats in normal control group EGF be expressed as the weak positive; Ulcer Models group is that strong positive is expressed, and this test modeling success is described.The cell that treatment group and positive treatment group seropositivity are expressed increases gradually.Chinese medicine oil wound dressing treatment group heavy dose and positive treatment group are expressed and are better than Normal group, difference has statistical significance (★ ★ P < 0.01, ★ P < 0.05), Chinese medicine oil wound dressing treatment group organizes comparing difference not statistically significant (P > 0.05) with positive.
This test adopts argon-gas knife calcination method to cause rat gastric ulcer model; treat with Chinese medicine oil wound dressing; adopt gastric mucosa protectant hydrotalcite tablet medicine in contrast; observe the therapeutic effect of Chinese medicine oil wound dressing; found that; Chinese medicine oil wound dressing and hydrotalcite tablet all have the effect of protection gastric mucosa, and can promote the healing of ulcer.In order to inquire into its mechanism of action, this test also observes this medicine to EGF and bFGF two factors, and find EGF for strong positive is expressed in Ulcer Models, its content is the highest, also has positive expression in various degree in each dosage group for the treatment of.
BFGF is the weak positive in normal gastric mucosa, in model group rats gastric tissue, content is apparently higher than Normal group (P < 0.01), also all higher than normal group in each treatment group, middle dosage and heavy dose of group content the highest (P < 0.01).Prompting Chinese medicine oil wound dressing can promote the activity of bFGF in ulcer rat gastric tissue, increase its content, thus promotes the healing of ulcer.
EGF is a kind of endogenous material having gastric acid secretion inhibiting, promote epitheliosis, tissue repair and cytoprotection; very important effect is played from damage factor destruction, maintenance gastrointestinal mucosa integrity at protection gastric mucosa; be the molecule bFGF that plays an important role in cell proliferation and differentiation in recent years found be a kind of cationic polypeptide somatomedin be made up of 146 aminoacid; be a kind of single-stranded polynucleotides peptide, be present in normal gastric mucosa.It has short division, short differentiation, can promote angiogenesis and fetal development; In addition, bFGF is former as a kind of important mitogenesis, can directly act on tissue repair cell (as the fibroblast) cycle, makes cell cycle shortening conversion time, accelerates division and the increment of cell.Utilize Exogenous bFGF to treat peptic ulcer, the healing of ulcer surface can be promoted.There are some researches show that topical application Exogenous bFGF accelerates ulcer healing by promoting gastric ulcer tissue microvascular to be formed, bFGF specificity neutralizing antibody then significantly can reduce microvessel quantity and ulcer edge blood flow bottom ulcer, thus delays gastric ulcer healing process.At present about the research of endogenous bFGF also achieves certain achievement.Have the expression of scholar's research endogenous bFGF in gastric ulcer healing process, find it in the weak positive in normal gastric mucosa, acute stage is strong positive, and weakens gradually in its expression of healing phases.
This test argon-gas knife Burns in Rats gastric mucosa, causes Ulcer Models, adopts Chinese medicine oil wound dressing to treat, observe its therapeutic effect, detect EGF, bFGF two factors, study it repairs phase expression in ulcer, inquire into the mechanism of action of Chinese medicine oil wound dressing treatment gastric ulcer.Found that Chinese medicine oil wound dressing has good therapeutical effect to rat gastric ulcer, EGF, bFGF are weak positive expression in normal rabbit serum and gastric mucosa, repair phase positive expression in ulcer.
Conclusion:
1, illustrate that this Chinese medicine oil wound dressing preparation technology (pulverizing constant temperature to extract) is obviously better than traditional decoction pieces and decocts extraction process by efficient Liquid Detection content of berberine hydrochloride.
2, illustrate that Chinese medicine oil wound dressing has no side effect by above animal experiment.
3, for scald, damaging skin, damage infected wound all has good Promotive union effect.
4, for oral administration have good therapeutical effect to the rat gastric ulcer caused by argon-gas knife.
In sum, the present invention for raw material, changes traditional preparation technology with ten plurality of Chinese, put into quantitative vegetable oil with the Chinese medicine powder after pulverizing to stir, put into the liposoluble constituent that 70-80 DEG C of calorstat extracts Chinese medicine again, place after 48 hours and take out, elimination medicinal residues; The Oleum Radix Arnebiae (Oleum Radix Lithospermi) extracted with same method again mixes with the ratio of 10:1 both makes Chinese medicine oil dressing.The method fully can extract the liposoluble constituent in medicine, and then play effect of compound medicine, through the clinical trial of example up to a hundred, evident in efficacy, the various wound being difficult to heal can be treated, as: the wound, lymphoid tuberculosis, serosity mastitis, ulcer, drug sensitivity dermatitis, steroid-dependent dermatitis, dermatitis traumatica, various burn, scald etc. of difficult healing after wound, chemicotherapy after dog bite, pressure ulcer, diabetic foot, lupus erythematosus, kidney transfer operation.
The crude drug that medicine of the present invention adopts easily is drawn materials, and can make oil preparation, medicine oil gauze etc. by technique, manufactures, stores, transports and use all very convenient.
Claims (3)
1. be used for the treatment of a Chinese medicinal formulae for wound, it is characterized in that, be made up of the component A of 10 ~ 30 mass parts and the B component compatibility of 3 ~ 10 mass parts;
Described component A, in mass fraction, be made up of following material combination: Radix Sanguisorbae 15 ~ 35 parts, the Radix Angelicae Dahuricae 10 ~ 40 parts, Radix Et Rhizoma Rhei 5 ~ 30 parts, Rhizoma Polygoni Cuspidati 15 ~ 40 parts, Radix Angelicae Sinensis 10 ~ 50 parts, Radix Rehmanniae 15 ~ 35 parts, Rhizoma Coptidis 10 ~ 45 parts, Cortex Phellodendri 15 ~ 35 parts, Radix Scutellariae 20 ~ 35 parts;
Described B component, in mass fraction, is made up of following material combination: Radix Arnebiae (Radix Lithospermi) 5 ~ 15 parts, Borneolum Syntheticum 2 ~ 5 parts, 3 ~ 10 parts, Cera Flava.
2. a kind of Chinese medicinal formulae being used for the treatment of wound according to claim 1, is characterized in that:
Described component A, in mass fraction, is made up of following material combination: Radix Sanguisorbae 30 parts, the Radix Angelicae Dahuricae 30 parts, Radix Et Rhizoma Rhei 30 parts, Rhizoma Polygoni Cuspidati 30 parts, Radix Angelicae Sinensis 30 parts, Radix Rehmanniae 30 parts, Rhizoma Coptidis 30 parts, Cortex Phellodendri 30 parts, Radix Scutellariae 30 parts;
Described B component, in mass fraction, is made up of following material combination: Radix Arnebiae (Radix Lithospermi) 10 parts, Borneolum Syntheticum 3 parts, 5 parts, Cera Flava.
3. adopt the Chinese medicinal formulae being used for the treatment of wound described in claim 1 or 2 to prepare the method for Chinese medicine oil dressing, it is characterized in that, comprise the following steps:
1) by prescription proportioning, pulverize after each for component A taste medicine mixing, then according to the solid-liquid ratio of 1g:3 ~ 5mL, the component A medicated powder after pulverizing is added in vegetable oil, stir, at 70 ~ 80 DEG C, process 24 ~ 48h, elimination medicinal residues, obtained component A medicine oil;
2) by prescription proportioning, pulverize after each for B component taste medicine mixing, then according to the solid-liquid ratio of 1g:30 ~ 50mL, the B component medicated powder after pulverizing is added in vegetable oil, stir, at 80 ~ 100 DEG C, process 24 ~ 48h, elimination medicinal residues, obtained B component medicine oil;
3) after component A medicine oil and B component medicine oil being pressed the mass ratio mixing of 10:1, obtained Chinese medicine oil dressing.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105902677A (en) * | 2016-06-01 | 2016-08-31 | 杨振铎 | Chinese medicinal ointment for treatment of postoperative non-healing wounds and preparation method thereof |
| CN108114113A (en) * | 2018-01-05 | 2018-06-05 | 付威 | A kind of ointment for treating wound |
| CN110292602A (en) * | 2019-08-16 | 2019-10-01 | 南昌大学第一附属医院 | Yellow burn oil formula of a kind of compound five and preparation method thereof |
| CN110585243A (en) * | 2019-08-12 | 2019-12-20 | 丰泽康生物医药（深圳）有限公司 | Pluripotent cell active matter and platelet-rich plasma compound for treating glucocorticoid-dependent dermatitis as well as preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104189187A (en) * | 2014-08-12 | 2014-12-10 | 刘学勤 | Externally applied spray for treating burns and scalds |
-
2015
- 2015-07-10 CN CN201510405427.4A patent/CN104983891B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104189187A (en) * | 2014-08-12 | 2014-12-10 | 刘学勤 | Externally applied spray for treating burns and scalds |
Non-Patent Citations (3)
| Title |
|---|
| 周永坤等: "《实用中医外科金方》", 30 April 2008 * |
| 无: "烫伤膏治疗药物过敏严重剥脱性皮炎2例的护理", 《考试吧》 * |
| 韦东: "中医药治疗烧伤的研究及其进展", 《辽宁中医学院学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105902677A (en) * | 2016-06-01 | 2016-08-31 | 杨振铎 | Chinese medicinal ointment for treatment of postoperative non-healing wounds and preparation method thereof |
| CN108114113A (en) * | 2018-01-05 | 2018-06-05 | 付威 | A kind of ointment for treating wound |
| CN110585243A (en) * | 2019-08-12 | 2019-12-20 | 丰泽康生物医药（深圳）有限公司 | Pluripotent cell active matter and platelet-rich plasma compound for treating glucocorticoid-dependent dermatitis as well as preparation method and application thereof |
| CN110292602A (en) * | 2019-08-16 | 2019-10-01 | 南昌大学第一附属医院 | Yellow burn oil formula of a kind of compound five and preparation method thereof |
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