CN104973694A - Production method of microbial preparation for water quality control - Google Patents
Production method of microbial preparation for water quality control Download PDFInfo
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- CN104973694A CN104973694A CN201410136779.XA CN201410136779A CN104973694A CN 104973694 A CN104973694 A CN 104973694A CN 201410136779 A CN201410136779 A CN 201410136779A CN 104973694 A CN104973694 A CN 104973694A
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Abstract
The invention discloses a production method of a microbial preparation for water quality control. The production method comprises the following steps: (1) fetching the following components in percentage by mass for later use: 0.5-1% of brown sugar, 0.5-1% of em strain, 0.9-1.5% of culture medium and 97-98% of water; (2) dissolving 0.5-1% of brown sugar with hot water and adding cold water till normal temperature; (3) disinfecting and washing a culture container, putting the culture medium into the culture container, dissolving with the brown sugar water in the step (2), and pouring the remaining water in the step (1) into the culture container; (4) introducing the em strain, wrapping the em strain with gauze, and culturing in the culture container; and (5) after the culture, taking out the strain, uniformly stirring the liquid and filling to obtain the microbial preparation. The preparation disclosed by the invention can effectively kill or inhibit of growth and propagation of the pathogenic microorganisms to promote the activation and growth of good bacteria and good algae, and can efficiently decompose organic components such as residual feed and excreta to improve the water quality and fundamentally maintain ecological balance in aquaculture water.
Description
Technical field:
The present invention relates to a kind of technical field of microbial preparation, more specifically relate to a kind of technical field of the microbial preparation for regulating and controlling water quality.
Background technology:
China is the big country of aquaculture.Aquaculture is that economic growth and fisherman's increased income make a great contribution.But the quality of water quality management is the important factor determining aquaculture success or not.Because the cultivation density propagated artificially is higher, the throwing something and feeding of artificial diet, the comparatively simple ecosystem and too low environmental carrying capacity very easily cause the Rapid Accumulation of toxic pollutant in water body, cause water quality deterioration.Water is changed in normal employing at present, purification of water quality medicine (sorbent material, oxygenant etc.) of splashing, use circulating water culture system etc. carry out regulating and controlling water quality.The discharge not only wasting manpower and material resources of a large amount of breeding wastewater, increases aquaculture cost, and contaminate environment cause the propagation on a large scale of disease.A large amount of uses of chemicals then destroy water ecosystem and have certain toxic action to aquaculture organism, very easily cause vicious cycle.Therefore, needs develop environmental protection more and effective regulating and controlling water quality method.
Summary of the invention:
For the problems referred to above, the object of the present invention is to provide a kind of environmental protection and effectively for the production method of the microbial preparation of regulating and controlling water quality.
The present invention is achieved by the following technical solutions.
For a production method for the microbial preparation of regulating and controlling water quality, comprise the steps:
(1) each component is got by following mass percent for subsequent use: brown sugar: 0.5-1%, em bacterial classification: 0.5-1%, substratum: 0.9-1.5%, water: 97-98%;
(2) by 0.5-1% brown sugar with after hot water dissolving, then add cold water to normal temperature;
(3) after culture vessel sterilization being rinsed, get substratum and put into culture vessel, then use the brown sugar water in step (2) to dissolve, more remaining water step (1) got is poured into culture vessel;
(4) access em bacterial classification, em bacterial classification is bound up with gauze, puts into culture vessel, make culture-liquid temp between 30-35 DEG C, cultivate 40-60 hour;
(5) cultivate end, taken out by bacterial classification, liquid stirs evenly filling, for the microbial preparation of regulating and controlling water quality.
Preferred as this technical scheme, described water is the one in sterilized water or phreatic water.
Preferred as this technical scheme, the sterilization method of described culture vessel is the alcohol of employing 75% or adds potassium permanganate with formalin and fumigate 2-4 hour.
Beneficial effect: preparation of the present invention can effectively kill or suppress the growth and breeding of pathogenic micro-organism, propagation beneficial microorganism and planktonic organism quantity, improve aquatic animal intestinal environment, improve absorptive function and the diseases prevention anti-adversity ability of aquatic animal, significantly reduce drug use amount, increase water body dissolved oxygen amount simultaneously, eliminate, degradation of ammonia nitrogen, hydrogen sulfide, the objectionable impuritiess such as nitrite, balance, stablize potential of hydrogen, promote profitable strain and beneficial algae activated growth, and the residual bait of efficient-decomposition, the organic compositions such as movement, eliminate mud, thus improve water quality, stablize water colour, make aquaculture water fertilizer tender, clearly, fundamentally keep the eubiosis in aquaculture water.
Embodiment:
The following stated is only the preferred embodiment embodying object of the present invention, effect and technical characteristic, does not therefore limit protection scope of the present invention.
Embodiment 1:
Get brown sugar: O.8g, em bacterial classification: O.5g, substratum: O.93g, sterilized water: 97.77g.Get 5g sterilized water and be heated to 80 DEG C, brown sugar is added and wherein dissolves, add 45g sterilized water after having dissolved and stir; Get culture dish, add with formalin that potassium permanganate is stifling to carry out disinfection for 4 hours, then with for subsequent use after aseptic water washing; The substratum of 0.93g is joined in culture dish, dissolve with brown sugar water, more remaining sterilized water is joined in culture dish, after stirring, access em bacterial classification, em bacterial classification is bound up with gauze with two-layer, puts into nutrient solution, keep the temperature of nutrient solution at 35 DEG C, cultivate after 48 hours, bacterial classification bag is taken out, stirs canned, obtain the microbial preparation for regulating and controlling water quality.
Embodiment 2:
Get brown sugar: O.5g, em bacterial classification: O.55g, substratum: O.95g, sterilized water: 98g.Get 5g sterilized water and be heated to 80 DEG C, brown sugar is added and wherein dissolves, add 45g sterilized water after having dissolved and stir; Get culture dish, add with formalin that potassium permanganate is stifling to carry out disinfection for 4 hours, then with for subsequent use after aseptic water washing; The substratum of 0.95g is joined in culture dish, dissolve with brown sugar water, more remaining sterilized water is joined in culture dish, after stirring, access em bacterial classification, em bacterial classification is bound up with gauze with two-layer, puts into nutrient solution, keep the temperature of nutrient solution at 30 DEG C, cultivate after 50 hours, bacterial classification bag is taken out, stirs canned, obtain the microbial preparation for regulating and controlling water quality.
Claims (3)
1. for a production method for the microbial preparation of regulating and controlling water quality, it is characterized in that, comprise the steps:
(1) each component is got by following mass percent for subsequent use: brown sugar: 0.5-1%, em bacterial classification: 0.5-1%, substratum: 0.9-1.5%, water: 97-98%;
(2) O.5-1% with after hot water dissolving, then cold water will be added to normal temperature for brown sugar;
(3) after culture vessel sterilization being rinsed, get substratum and put into culture vessel, then use the brown sugar water in step (2) to dissolve, more remaining water step (1) got is poured into culture vessel;
(4) access em bacterial classification, em bacterial classification is bound up with gauze, puts into culture vessel, make culture-liquid temp between 30-35 DEG C, cultivate 40-60 hour;
(5) cultivate end, taken out by bacterial classification, liquid stirs evenly filling, obtains the microbial preparation for regulating and controlling water quality.
2. the production method of a kind of microbial preparation for regulating and controlling water quality according to claim l, is characterized in that, described water is the one in sterilized water or phreatic water.
3. the production method of a kind of microbial preparation for regulating and controlling water quality according to claim 1, is characterized in that, the sterilization method of described culture vessel is the alcohol disinfecting of employing 75% or adds potassium permanganate with formalin and fumigate 2-4 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410136779.XA CN104973694A (en) | 2014-04-02 | 2014-04-02 | Production method of microbial preparation for water quality control |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410136779.XA CN104973694A (en) | 2014-04-02 | 2014-04-02 | Production method of microbial preparation for water quality control |
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| Publication Number | Publication Date |
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| CN104973694A true CN104973694A (en) | 2015-10-14 |
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| CN201410136779.XA Pending CN104973694A (en) | 2014-04-02 | 2014-04-02 | Production method of microbial preparation for water quality control |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105399219A (en) * | 2015-12-23 | 2016-03-16 | 太仓东浔生物科技有限公司 | Water quality regulation and control compound microbial preparation and production method thereof |
| CN106430625A (en) * | 2016-10-21 | 2017-02-22 | 吴迪 | Preparation method of ecological and environment-friendly composite biological algaecide |
-
2014
- 2014-04-02 CN CN201410136779.XA patent/CN104973694A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105399219A (en) * | 2015-12-23 | 2016-03-16 | 太仓东浔生物科技有限公司 | Water quality regulation and control compound microbial preparation and production method thereof |
| CN106430625A (en) * | 2016-10-21 | 2017-02-22 | 吴迪 | Preparation method of ecological and environment-friendly composite biological algaecide |
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Addressee: HUZHOU RONGQIA BIOTECHNOLOGY CO., LTD. Document name: Notification of before Expiration of Request of Examination as to Substance |
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Application publication date: 20151014 |