CN104971345A - Novel emodin gelatin microsphere and nano-silver composite gel preparation for treating burn injury and scald injury - Google Patents
Novel emodin gelatin microsphere and nano-silver composite gel preparation for treating burn injury and scald injury Download PDFInfo
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- CN104971345A CN104971345A CN201510368421.4A CN201510368421A CN104971345A CN 104971345 A CN104971345 A CN 104971345A CN 201510368421 A CN201510368421 A CN 201510368421A CN 104971345 A CN104971345 A CN 104971345A
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Abstract
The invention discloses a novel emodin gelatin microsphere and nano-silver composite gel preparation for treating burn injury and scald injury and a preparation method therefore. The novel emodin gelatin microsphere and nano-silver composite gel preparation for treating burn injury and scald injury is novel complex gel prepared from the following components according to a weight ratio: 43.1 parts of emodin gelatin microsphere, 0.2 part of nano-silver, 3.4 parts of fish scale collagen and 953.2 part of Carbomer substrate. The preparation provided by the invention is a water-based gel preparation and has the advantages of being attractive, high in comfortability in use, good in biocompatibility, high in bioavailability, good in stability, less in adverse reaction and not polluting clothing.
Description
Technical field
The present invention relates to a kind of NEW TYPE OF COMPOSITE gel being used for the treatment of burn and scald and preparation method thereof.
Background technology
Burn and scald is the wound that people often can run in daily life, and as processed not in time, wound can form congestion, cause inflammation generation pain, and wound can be infected by bacterial and increase the weight of traumatic condition, even entail dangers to life.At present, antibiotic therapy is the conventional method of doctor trained in Western medicine, though there is certain curative effect, but Wound Contraction is slow, and long-term prescription can produce drug resistance, makes medicine ineffective, even can make damage Liver and kidney etc. and then cause body's immunity to reduce.And the externally applied ointment that the traditional Chinese medical science adopts has unique curative effect in treatment wound.The plaster for the treatment of burn and scald more prevailing on market has JINGWANHONG RUANGAO, general Ji burn cream, ice king ointment for treating burn and scald etc., and these plaster respectively have pluses and minuses.JINGWANHONG low price, treatment burn and scald effect is better, but is mainly used in treating slight burn and scald, to deep burn poor effect; General Ji burn cream treatment burning effect is good, and can treat degree of depth large area burn and scald, but expensive; Ice king ointment for treating burn and scald low price, be mainly used in slight burn and scald, and curative effect is slower.
Emodin has the pharmacological actions such as antibacterial anti-inflammatory, blood circulation promoting and blood stasis dispelling, removing the necrotic tissue and promoting granulation.Nanometer silver is natural antibacterial material of new generation, has that broad-spectrum antiseptic, permeability are strong, reparative regeneration, safety non-toxic, the pharmacology such as have no drug resistance, and the height of tiring of silver, few nanometer silver can produce powerful drug effect, and cost is low.Collagen protein is a kind of biopolymer protein, has good fiber reshaping, is easily absorbed by the body, can promote coagulating platelets, promotes cellular activities and growth, promotes wound healing.The collagen protein of molecular weight below 1000 dalton can be directly absorbed by the body without the need to decomposing, and is a kind of well medical material.Aqueous gel is development in recent years dosage form faster, have attractive in appearance, use comfortable, biological utilisation is high, good stability, untoward reaction are few, the not advantage such as pollution clothes.
Summary of the invention
The present invention is intended to develop a kind of good effect, and production cost is low, and can treat the aqueous gel preparation of various burn and scald.
The present invention is a kind of NEW TYPE OF COMPOSITE gel for the treatment of burn and scald, and it is the NEW TYPE OF COMPOSITE gel be made up of the composition of following weight ratio:
Emodin gelatine microsphere 43.1 parts of nanometer silvers 0.2 part
Fish scale collagen 3.4 parts of carbomer substrates 953.3 parts
The preparation method of the NEW TYPE OF COMPOSITE gel of described treatment burn and scald, comprises the following steps:
The first step: extract collagen protein from fish scale, for subsequent use;
Second step: prepare emodin gelatine microsphere, for subsequent use;
3rd step: prepare carbomer substrate, for subsequent use;
4th step: the nanometer silver suspension getting the ultrasonic 20min of nanometer silver adding distil water, for subsequent use;
5th step: the nanometer silver suspension of the carbomer substrate of the emodin gelatine microsphere of the fish scale collagen of the above-mentioned first step, second step, the 3rd step and the 4th step is mixed, stirs, make NEW TYPE OF COMPOSITE gel.
In described method, emodin, not react between nanometer silver and fish scale collagen three, triplicity is made NEW TYPE OF COMPOSITE gel, be applied on common burn and scald, can not only functions of detumescence, relieving inflammation, blood circulation promoting and blood stasis dispelling, reparative regeneration, the wound infection that can also cause microorganism plays good bactericidal action.
fig. 1the pathological section figure of wound tissue during each group of mice 1.5d;
fig. 2the pathological section figure of wound tissue when being experimental mice 3d;
fig. 3the pathological section figure of wound tissue when being control group mice 3d;
fig. 4the pathological section figure of wound tissue during naive mice 3d;
fig. 5the pathological section figure of wound tissue when being experimental mice 9d;
fig. 6the pathological section figure of wound tissue when being control group mice 9d;
fig. 7the pathological section figure of wound tissue during naive mice 9d;
fig. 8the pathological section figure of wound tissue when being experimental mice 21d;
fig. 9the pathological section figure of wound tissue when being control group mice 21d;
fig. 1the pathological section figure of 0 wound tissue when being naive mice 21d;
fig. 11 is the pathological section figure of wound site after each group of mice wound heals completely.
Specific embodiments
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1: the preparation method of the NEW TYPE OF COMPOSITE gel for the treatment of burn and scald comprises the following steps:
The first step: extract collagen protein from fish scale: weigh fish scale 20g, adding distil water magnetic stirrer 0.5h, removing impurity, then clean up with distilled water; Add 200mL 0.6mol/L hydrochloric acid solution after drying to soak, use magnetic stirrer 5h; Be washed till without suspended impurity with distilled water, after drying, add 200mL 0.1mol/L hydrochloric acid solution, 0.8g pepsin, be placed in fridge freshness retaining room (temperature is 4 DEG C) and place enzymolysis 24h; By the fish scale filtered through gauze after enzymolysis, in filtrate, add 10g sodium chloride, leave standstill 6h; The rotating speed of TGL-16 high speed centrifuge is adjusted to 5000r/min, by centrifugal for above-mentioned filtrate 20min, inclines and supernatant, take out off-white color precipitation in centrifuge tube, obtain fish scale collagen.
Second step: prepare emodin gelatine microsphere: add 0.8g sorbester p17 in 50ml liquid paraffin, is preheated to 60 DEG C, the rotating speed of magnetic stirring apparatus is adjusted to 400rpm, stirs; Get 20% (w/v) gelatin solution 5ml, be preheated to 60 DEG C, add in aforesaid liquid paraffin, stir 15min; Get 0.1g emodin to be dissolved in 5ml ethanol, be preheated to 60 DEG C, add in above-mentioned mixing material, stir 15min, and be cooled to rapidly 5 DEG C, centrifugal obtained emodin gelatine microsphere.
3rd step: prepare carbomer substrate: get carbomer 0.5g, glycerol 40ml, triethanolamine 0.45ml, 1% sodium benzoate aqueous solution 2ml mix homogeneously, obtained substrate.
4th step: get nanometer silver 0.06g, adding distil water 12ml, water bath sonicator 20min, obtain nanometer silver suspension.
5th step: the nanometer silver suspension mixing that obtained carbomer substrate 278.6g and the 4th step in emodin gelatine microsphere 12.6g obtained in the fish scale collagen 1g obtained in the above-mentioned first step, second step, the 3rd step are obtained, stir, use magnetic stirrer 1h again, namely make NEW TYPE OF COMPOSITE gel.
Embodiment 2: with the NEW TYPE OF COMPOSITE gel made, carry out mouse experiment, investigate its curative effect.Specific experiment method is as follows:
Get 24 Kunming mouses, male, about 25g, is divided into 3 groups, is numbered one, two, three group, often organizes 8.Adopt the method for continuous narcosis, after etherization mice, shave the hair of clean mouse back with special shaver, make its skin exposed.Mice is placed in ventricumbent position, extremity stretch, with clip, its extremity are fixed on operation panel, skin of back is made to be flat condition, with mice spinal column for center line, with little iron block (area is 1cm × 1cm) the burned mouse scapula rear from room temperature heating 3min, symmetrical position is each scalds a wound, firmly moderate, the time is 5s.
After scalding model is made, first group of mice left-hand component smears 0.9% normal saline, and the right wound smears NEW TYPE OF COMPOSITE gel; NEW TYPE OF COMPOSITE gel is smeared on second group of mice left side, and Ointment (pharmacy's purchase) is smeared on the right; Ointment is smeared on the 3rd group of mice left side, and 0.9% normal saline is smeared on the right.Once a day, conscientiously mice is observed.Concrete observation index is as follows:
1, gross examination of skeletal muscle: observe mice mental status, feed, sleep and active situation.
2, each group of mouse skin infective wound surface is examined, the change of record wound surface color, oozes out with or without purulent secretion, whether occur ulcer, bleed, the phenomenon such as incrustation.
3, by mice with after ether daze, with 0.1cm × 0.1cm standard transparent calculate scale paper be covered on wound, carry out the percentile mensuration of wound healing.
Healing rate=[(A
n-1-A
n)/A
n-1] × 100%
A in formula
n-1=scald latter (n-1)th day wound surface area, A
n=postoperative n-th day wound surface area (open wound).
Result of calculation is in table one:
Table one
The healing rate that can be obtained NEW TYPE OF COMPOSITE gel by table one result is better than JINGWANHONG and normal saline.
Embodiment 3: get mice one respectively at 1.5d, 3d, 9d, 21d, completely latter each group of healing, get wound tissue, fix with 4% paraformaldehyde, transfer to Medical University Of Chongqing to make pathological section, observe, observed result is as follows:
1.5d: three groups there is no too big difference, and inflammatory cell quantity is very nearly the same.As Fig. 1.
3d: the relatively blank group of experimental mice wound tissue inflammatory cell quantity and matched group less, and have fibroblast to generate, as Fig. 2; Control group mice wound tissue inflammatory cell quantity is maximum, as Fig. 3; Naive mice wound tissue inflammatory cell quantity is very many, as Fig. 4.
9d: experimental group inflammatory cell quantity relative to blank group and matched group less, fibroblast increasing number, and have angeogenesis, as Fig. 5; Matched group inflammatory cell quantity is very many, has fibroblast to generate, as Fig. 6; Blank group inflammatory cell quantity is very many, has no fibroblast and generates, as Fig. 7.
21d: experimental group grows granulation tissue, quantity is more, as Fig. 8; Matched group granulation tissue negligible amounts, as Fig. 9; Blank group granulation tissue quantity is more, as Figure 10.
Completely after healing: three groups of situations are similar, skin histology level is distinct, and inside is visible a small amount of inflammatory cell all still, as Figure 11.
Embodiment 4: after NEW TYPE OF COMPOSITE gel is made after 5d, 65d, 130d, carries out study on the stability to the Chinese medicine ingredients in NEW TYPE OF COMPOSITE gel, identifies whether it goes bad.Get NEW TYPE OF COMPOSITE gel 5g, add methanol 20ml, water bath sonicator 20min, filter, concentrated, with thin layer chromatography qualification (developing solvent: petroleum ether 60-90: ethyl acetate: formic acid=15: 5: 1).Observe under the uviol lamp of 254nm wavelength, calculate Rf value, the results are shown in Table two.
Table two
The present invention treats in burn and scald process, and mouse test results shows it to the healing rate of wound higher than JINGWANHONG RUANGAO and normal saline.
Should be understood that, for those of ordinary skills, can illustrate according to appeal and be improved or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (7)
1. treat a NEW TYPE OF COMPOSITE gel for burn and scald, it is characterized in that, it is the NEW TYPE OF COMPOSITE gel be made up of the composition of following weight ratio:
Emodin gelatine microsphere 43.1 parts of nanometer silvers 0.2 part
Fish scale collagen 3.4 parts of carbomer substrates 953.3 parts.
2. a preparation method for emodin gelatine microsphere as claimed in claim 1, is characterized in that, comprise the following steps:
The first step: add sorbester p17 in liquid paraffin, is preheated to 60 DEG C, stirs, for subsequent use;
Second step: 20% (w/v) gelatin solution, is preheated to 60 DEG C, adds in aforesaid liquid paraffin, stir, for subsequent use;
3rd step: get emodin and be dissolved in ethanol, preheating, add in above-mentioned mixed solution, stirs and is cooled to rapidly 5 DEG C, centrifugally namely obtains emodin gelatine microsphere.
3. emodin gelatine microsphere as claimed in claim 2, it is characterized in that, liquid paraffin in the described first step, second step: sorbester p17: 20% (w/v) gelatin solution=10: 0.1565: 1 (v/w/v), emodin in described 3rd step: ethanol=1: 50 (w/v).
4. an extracting method for fish scale collagen as claimed in claim 1, is characterized in that, comprises the following steps:
The first step: get fish scale remove impurity, adds 0.6mol/L hydrochloric acid solution and stirs 5h, clean up, dry for subsequent use;
Second step: above-mentioned fish scale is added 0.1mol/L hydrochloric acid solution, pepsin, is placed in fridge freshness retaining room and places enzymolysis 24h;
3rd step: filtered by the fish scale after above-mentioned enzymolysis, add sodium chloride in filtrate, after leaving standstill 6h, centrifugal 20min, inclines and supernatant, takes out off-white color in centrifuge tube and precipitates and obtain fish scale collagen.
5. fish scale collagen as claimed in claim 4, it is characterized in that, the described first step, second step and the 3rd step Raw ratio of reagents are fish scale: 0.6mol/L hydrochloric acid solution: 0.1mol/L hydrochloric acid solution: pepsin: sodium chloride=4.65: 46.42: 46.42: 0.19: 2.33 (w/v/v/w/w).
6. a preparation method for carbomer substrate as claimed in claim 1, is characterized in that, comprise the following steps:
Get carbomer according to a certain percentage, glycerol, triethanolamine, 1% sodium benzoate aqueous solution obtain substrate.
7. carbomer substrate as claimed in claim 6, it is characterized in that, its ratio is carbomer: glycerol: triethanolamine: 1% sodium benzoate aqueous solution=1.17: 93.14: 1.04: 4.66 (w/v/v/v).
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| CN109602897A (en) * | 2019-01-31 | 2019-04-12 | 重庆医药高等专科学校 | A kind of flamazine temperature-sensitive hydrogel and preparation method thereof |
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| CN109602897A (en) * | 2019-01-31 | 2019-04-12 | 重庆医药高等专科学校 | A kind of flamazine temperature-sensitive hydrogel and preparation method thereof |
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Effective date of registration: 20211202 Address after: 310000 2nd floor, building 1, No. 51, haoshantou, Chonghua village, Pingyao Town, Yuhang District, Hangzhou City, Zhejiang Province Patentee after: Saifu Maide medical technology (Hangzhou) Co.,Ltd. Address before: No.174, shazheng street, Shapingba, Chongqing Patentee before: Chongqing University |
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