CN103239540B - Cooling blood callus prescription extractive and compounded resveratrol cooling blood callus oil preparation for treating skin burn - Google Patents
Cooling blood callus prescription extractive and compounded resveratrol cooling blood callus oil preparation for treating skin burn Download PDFInfo
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Abstract
The invention relates to the field of medicines, and in particular relates to a cooling blood callus prescription extractive and a compounded resveratrol cooling blood callus oil preparation for treating skin burn. The callus prescription extractive comprises the following raw materials by weight: 30-50g of goldthread, 30-50g of golden cypress, 30-50g of radix scutellariae, 20-40g of rheum officinale, 20-40g of radix sophorae flavescentis, 20-40g of the root bark of shaggy-fruited dittany, 10-30g of periostracum cicada, 5-15g of cuttlebone, 50-80g of lithospermum, 20-40g of garden burnet, 10-30g of red flower and 10-20g of earthworm. The compounded resveratrol cooling blood callus oil preparation is prepared by combining the callus prescription extractive and resveratrol. The preparation has the anti-inflammatory and debridement effects, can not only cover the wound surface, reduce infection, but also can actively participate in the burn wound healing, thus greatly improving the treating effect of wound surfaces of burn, trauma and the like.
Description
Technical field
The present invention relates to medical technical field, more specifically, relate to the compound resveratrol removing heat from blood wound healing oil formulation of a kind of removing heat from blood wound healing formula extraction and treatment skin burn.
Background technology
Skin burn is common disease clinically, often with complication, often brings huge painful and inconvenience in life to patient, affects the external attractive in appearance of patient more, and severe patient can threaten patient's life.Thereby, actively promote wound repair to saving patient's life, reduce complication, alleviate patient suffering, prevent the formation of cicatrix, improve the aspects such as therapeutic effect and all have great importance.At present, in succession developed both at home and abroad medicine and the preparation of many treatment skin burns, some drugs effect is very definite, for clinical development new drug and preparation provide good thinking and basis.Be accompanied by bionics, the development of chemical industry synthetic technology, making to develop clinically better medicament material becomes possibility.Current, the pharmaceutical preparation that is used for the treatment of burn mostly is water preparation, unguentum (or oil extractum), powder, spray, cream etc., and its bioavailability is not high, and sensory trait is poor, and biocompatibility is not high, and moisturizing and slow release effect are poor, uses inconvenience etc.
Summary of the invention
Technical problem to be solved by this invention is, in order to overcome the medicine above shortcomings for the treatment of burn in prior art, to provide a kind of removing heat from blood wound healing formula extraction and preparation method thereof.
Another technical problem to be solved by this invention is that compound resveratrol removing heat from blood wound healing oil formulation of a kind for the treatment of skin burn that contains above-mentioned removing heat from blood wound healing formula extraction and preparation method thereof is provided.
Above-mentioned technical problem to be solved by this invention is achieved by the following technical programs:
A kind of removing heat from blood wound healing formula extraction, is extracted and is obtained by the raw material that comprises following consumption: Rhizoma Coptidis 30～50g, Cortex Phellodendri 30～50g, Radix Scutellariae 30～50g, Radix Et Rhizoma Rhei 20～40g, Radix Sophorae Flavescentis 20～40g, Cortex Dictamni 20～40g, Periostracum Cicadae 10～30g, Endoconcha Sepiae 5～15g, Radix Arnebiae (Radix Lithospermi) 50～80g, Radix Sanguisorbae 20～40g, Flos Carthami 10～30g, 10～20 of Pheretimas.
The above-mentioned raw material of Chinese medicine (removing heat from blood wound healing side) that the present invention screens through great many of experiments, the multiple alkaloid wherein containing and all the other Organic substances all have good antiinflammatory promoting healing and antioxidation, there is in addition hemostasis, the effect of activating blood circulation to dissipate blood stasis and enhancing wound surface resistance.
As a kind of preferred version, above-mentioned removing heat from blood wound healing formula extraction, is extracted and is obtained by the raw material that comprises following consumption: Rhizoma Coptidis 35～45g, Cortex Phellodendri 35～45g, Radix Scutellariae 35～45g, Radix Et Rhizoma Rhei 25～35g, Radix Sophorae Flavescentis 25～35g, Cortex Dictamni 25～35g, Periostracum Cicadae 15～25g, Endoconcha Sepiae 8～12g, Radix Arnebiae (Radix Lithospermi) 50～70g, Radix Sanguisorbae 25～35g, Flos Carthami 15～25g, 12～16 of Pheretimas.
As most preferably scheme of one, a kind of removing heat from blood wound healing formula extraction, is extracted and is obtained by the raw material that comprises following consumption: Rhizoma Coptidis 40g, Cortex Phellodendri 40g, Radix Scutellariae 40g, Radix Et Rhizoma Rhei 30g, Radix Sophorae Flavescentis 30g, Cortex Dictamni 30g, Periostracum Cicadae 20g, Endoconcha Sepiae 10g, Radix Arnebiae (Radix Lithospermi) 60g, Radix Sanguisorbae 30g, Flos Carthami 20g, 15 of Pheretimas.
The Radix Et Rhizoma Rhei (charred Radix Et Rhizoma Rhei or Radix et Rhizoma Rhei (stir-fried with wine)) that above-mentioned Radix Et Rhizoma Rhei can be Radix Et Rhizoma Rhei or concocted, above-mentioned Pheretima can be fresh and alive Pheretima, or the Pheretima of concocting.
The preparation method of above-mentioned removing heat from blood wound healing formula extraction is to obtain with the ethanol extraction that volume fraction is 40%～80%.
As a kind of preferred version, the preparation method of described removing heat from blood wound healing formula extraction, is prepared by the method that comprises following steps:
Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Cortex Dictamni, Periostracum Cicadae, Endoconcha Sepiae, Radix Arnebiae (Radix Lithospermi), Radix Sanguisorbae, Flos Carthami and Pheretima are pressed to above-mentioned weight co-grinding, add volume fraction and be ethanol 2000～4000ml of 40%～80%, controlling temperature is 80～95 DEG C, reflux, extract, 2～4 times, each 1～3h, filtrate is merged, concentrate and get final product.
As the further preferred version of one, the preparation method of described removing heat from blood wound healing formula extraction, is prepared by the method that comprises following steps:
S1. incite somebody to action: Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Cortex Dictamni, Periostracum Cicadae, Endoconcha Sepiae, Radix Arnebiae (Radix Lithospermi), Radix Sanguisorbae, Flos Carthami and Pheretima are by above-mentioned weight co-grinding, add volume fraction and be ethanol 2000～4000ml of 40%～80%, controlling temperature is 80～85 DEG C, reflux, extract, 1～3h, filter, obtain filtering residue 1 and filtrate 1;
S2. filtering residue 1 being added to volume fraction is ethanol 2000～4000ml of 40%～80%, and controlling temperature is 80～85 DEG C, and reflux, extract, 1～3h filters, and obtains filtering residue 2 and filtrate 2;
S3. filtering residue 2 being added to volume fraction is ethanol 2000～4000ml of 40%～80%, and controlling temperature is 80～85 DEG C, and reflux, extract, 1～2h filters, and obtains filtering residue 3 and filtrate 3;
S4. get filtrate 1 40 DEG C of rotary evaporations in Rotary Evaporators and extract 2～3h, obtain concentrated solution 1;
S5. get filtrate 2 60 DEG C of rotary evaporations in Rotary Evaporators and extract 1～2h, obtain concentrated solution 2;
S6. get filtrate 3, in Rotary Evaporators, 70 DEG C of rotary evaporations extract 1～2h, obtain concentrated solution 3;
S7. get concentrated solution 1,2,3, after mixing in Rotary Evaporators 80 DEG C of rotary evaporations, until ethanol evaporation is removed, to obtain final product.
As most preferably scheme of one, the preparation method of described removing heat from blood wound healing formula extraction, is characterized in that, is prepared by the method that comprises following steps:
S1. incite somebody to action: Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Cortex Dictamni, Periostracum Cicadae, Endoconcha Sepiae, Radix Arnebiae (Radix Lithospermi), Radix Sanguisorbae, Flos Carthami and Pheretima are by above-mentioned weight co-grinding, add volume fraction and be 60% ethanol 3000ml, controlling temperature is 80～85 DEG C, reflux, extract, 1～3h, filter, obtain filtering residue 1 and filtrate 1;
S2. filtering residue 1 being added to volume fraction is 60% ethanol 3000ml, and controlling temperature is 80～85 DEG C, and reflux, extract, 2h filters, and obtains filtering residue 2 and filtrate 2;
S3. filtering residue 2 being added to volume fraction is 60% ethanol 3000ml, and controlling temperature is 80～85 DEG C, and reflux, extract, 1h filters, and obtains filtering residue 3 and filtrate 3;
S4. get filtrate 1 40 DEG C of rotary evaporations in Rotary Evaporators and extract 2～3h, obtain concentrated solution 1;
S5. get filtrate 2 60 DEG C of rotary evaporations in Rotary Evaporators and extract 1～2h, obtain concentrated solution 2;
S6. get filtrate 3, in Rotary Evaporators, 70 DEG C of rotary evaporations extract 1～2h, obtain concentrated solution 3;
S7. get concentrated solution 1,2,3, after mixing in Rotary Evaporators 80 DEG C of rotary evaporations, until ethanol evaporation is removed, to obtain final product.
A compound resveratrol removing heat from blood wound healing oil formulation for the treatment of skin burn, comprises following component: removing heat from blood wound healing formula extraction of the present invention 1000～2000ml; High mountain Oleum Camelliae 1000～2000ml; Borneolum Syntheticum 5～10g; Resveratrol 3.04～9.12mg; Penetrating agent: 4～20ml; Antiseptic: 1～30ml.
As a kind of preferred version, described penetrating agent is azone, and antiseptic is vitamin E.
The preparation method of above-mentioned compound resveratrol removing heat from blood wound healing oil formulation, is characterized in that, comprises the steps:
S1. after the filter residue and drying after claim 4～7 being extracted, mix with 1000～2000ml high mountain Oleum Camelliae, controlling temperature is 85～90 DEG C, and reflux, extract, 2～4h filters, and obtains filtrate 4;
S2. get filtrate 4 and removing heat from blood wound healing formula extraction 60～70 DEG C of rotary evaporation 2h in Rotary Evaporators, add Borneolum Syntheticum stirring and dissolving in above-mentioned mixed liquor, obtain removing heat from blood wound healing oil;
S3. get resveratrol and be dissolved in removing heat from blood wound healing oil, obtain compound resveratrol removing heat from blood wound healing oil; Add penetrating agent, antiseptic is uniformly mixed again, and to obtain final product.
As a kind of preferred version, described penetrating agent is azone, and antiseptic is vitamin E.
The preparation method of above-mentioned compound resveratrol removing heat from blood wound healing oil formulation is for to add a Borneolum Syntheticum at removing heat from blood wound healing prescription extract, and stirring and dissolving, then adds resveratrol, finally adds azone, vitamin E to be uniformly mixed and to get final product.
The raw material of Chinese medicine in removing heat from blood wound healing extract is passed through ethanol by the present invention, two kinds of organic solvents of high mountain Oleum Camelliae, evaporate respectively reflux, extract,, and it is concentrated to carry out roguing by Rotary Evaporators, obtain removing heat from blood wound healing formula extraction, in extract, add and there is obvious antioxidation again, promote the resveratrol of cytothesis to make the oily preparation after improvement.Research shows: resveratrol has anti-damage, antioxidation, and can have very strong short division and the effect of propagation to zooblast under doses.In repairing, burn can be good at promoting skin and other histiocytic growth and propagation, the expression of the various cytokines of energy normal regulation, thus accelerate the healing of wound surface, improve the quality of wound repair.In addition, oiliness environment promotes that the advantage of wound healing is that oil is higher with skin histology degree of adhesion, guarantee that sustained drug acts on burn wound, and be conducive to the particularly alkaloidal absorption of oil-soluble and utilization of medicine, and the long-time gentle wound surface of moisturizing of energy, wound healing, and can prevent incrustation and the formation of cicatrix.Wound is conducive to the reparation of tissue in the debridement phase, accelerate dissolving and the absorption of slough.Form the phase in granulation tissue, can promote the release of various somatomedin, stimulate the regeneration of blood capillary.In the epithelization phase, the speed that epidermis cell is divided a word with a hyphen at the end of a line in oiliness moisturizing environment is accelerated, and has the effect of rapid reparation corium.Oiliness heals in wound healing process, accelerating wound healing, and the treatment time of shortening wound, reduce dressing change frequency, save dressing, alleviate the pain of wound, and reduced medical expense, reduce nursing work load simultaneously.The desirable dressing for the treatment of burn should possess following functions: prevent excessively scattering and disappearing of moisture and body fluid; Resist the invasion of antibacterial, protect from infection; Good with wound surface stickiness, medicine can continuingly act on wound surface, increases wound surface to the absorbing of medicine, and breathe freely and make wound surface in moistening but there is no the environment of hydrops, good biocompatibility, and preferably can there is the function that can promote wound healing.
Compared with prior art, the present invention has following beneficial effect:
(1) preparation of the present invention, there is antiinflammatory, debridement effect, can keep the oiliness environment of wound surface, and create antioxidation environment, promote the release of angeogenesis and somatomedin, can not only flap coverage, reduce and infect, and can also initiatively participate in burn wound healing, thereby greatly improve the therapeutic effect of the wound surface such as burn, wound; (2) the present invention's effective component extracting from removing heat from blood wound healing side, in conjunction with resveratrol, makes Oily preparation, compared with the unguentum of similar drugs preparation, powder, spray, cream etc., has the feature of the combination of Chinese and Western medicine; (3) to have preparation method simple, easy to use in the present invention, is convenient to skin histology infiltration and absorbs, and has good skin-friendliness, and burn window is had to moisturizing slow release effect, nontoxic non-stimulated, fine and smooth attractive in appearance, the advantages such as stability and safety.
Detailed description of the invention
Further explain the present invention below in conjunction with specific embodiment, but embodiment does not limit in any form to invention.
Embodiment 1
Take Rhizoma Coptidis 40g, Cortex Phellodendri 40g, Radix Scutellariae 40g, Radix Et Rhizoma Rhei 30g, Radix Sophorae Flavescentis 30g, Cortex Dictamni 30g, Periostracum Cicadae 20g, Endoconcha Sepiae 10g, Radix Arnebiae (Radix Lithospermi) 60g, Radix Sanguisorbae 30g, Flos Carthami 20g, 15 of Pheretimas, 6 grams of Borneolum Syntheticums, medical material is all the other co-grindings except Borneolum Syntheticum, cross 10 molecules of interest orders, add 60% ethanol 3000ml, 80～85 DEG C of reflux, extract, 3h in round-bottomed flask.Then medicinal powder and alcohol mixture are filtered, obtain filtering residue 1, filtrate 1.Get filtering residue 1 and add 60% ethanol 3000ml, 80～85 DEG C of reflux, extract, 2h in round-bottomed flask.Then filtering residue and alcohol mixture are filtered, obtain filtering residue 2, filtrate 2.Get filtering residue 2 and add 60% ethanol 3000ml, 80～85 DEG C of reflux, extract, 1h in round-bottomed flask.Then filtering residue and alcohol mixture are filtered, obtain filtering residue 3, filtrate 3.
Get filtering residue 3 and be placed in natural environment logical dry in the sun wind and dry after 24h, add high mountain Oleum Camelliae 1500ml 90 DEG C of reflux, extract, 3.5h in round-bottomed flask.Then filtering residue and high mountainous tea oil mixture are filtered, obtain filtering residue 4, filtrate 4.Get filtrate 1 40 DEG C of rotary evaporations in Rotary Evaporators and extract 2～3h, obtain concentrated solution 1.Get filtrate 2 60 DEG C of rotary evaporations in Rotary Evaporators and extract 1～2h, obtain concentrated solution 2.Get filtrate 3, in Rotary Evaporators, 70 DEG C of rotary evaporations extract 1～2h, obtain concentrated solution 3.Get concentrated solution 1,2,3, after mixing in Rotary Evaporators 80 DEG C of rotary evaporations, until ethanol evaporation is removed, obtain about 1500ml removing heat from blood wound healing formula extraction.Get filtrate 4 and removing heat from blood wound healing formula extraction 60～70 DEG C of rotary evaporation 2h in Rotary Evaporators, the mixture of filtrate 4 and removing heat from blood wound healing formula extraction is taken out from distilling flask, and add 6g Borneolum Syntheticum stirring and dissolving in above-mentioned mixed liquor, obtain about 1500ml removing heat from blood wound healing oil.Get resveratrol 4.56mg and be dissolved in removing heat from blood wound healing oil, obtain compound resveratrol removing heat from blood wound healing oil.Add the about 6.25ml of azone, the about 10mg of vitamin E is uniformly mixed, and can use again.
Embodiment 2
Take Rhizoma Coptidis 30g, Cortex Phellodendri 50g, Radix Scutellariae 30g, Radix Et Rhizoma Rhei 40g, Radix Sophorae Flavescentis 40g, Cortex Dictamni 40g, Periostracum Cicadae 25g, Endoconcha Sepiae 15g, Radix Arnebiae (Radix Lithospermi) 80g, Radix Sanguisorbae 40g, Flos Carthami 30g, 20 of Pheretimas, 10 grams of Borneolum Syntheticums, medical material is all the other co-grindings except Borneolum Syntheticum, cross 10 molecules of interest orders, add 80% ethanol 4000ml, 80～85 DEG C of reflux, extract, 3h in round-bottomed flask.Then medicinal powder and alcohol mixture are filtered, obtain filtering residue 1, filtrate 1.Get filtering residue 1 and add 80% ethanol 4000ml, 80～85 DEG C of reflux, extract, 2h in round-bottomed flask.Then filtering residue and alcohol mixture are filtered, obtain filtering residue 2, filtrate 2.Get filtering residue 2 and add 80% ethanol 4000ml, 80～85 DEG C of reflux, extract, 1h in round-bottomed flask.Then filtering residue and alcohol mixture are filtered, obtain filtering residue 3, filtrate 3.
Get filtering residue 3 and be placed in natural environment logical dry in the sun wind and dry after 24h, add high mountain Oleum Camelliae 2000ml 90 DEG C of reflux, extract, 3.5h in round-bottomed flask.Then filtering residue and high mountainous tea oil mixture are filtered, obtain filtering residue 4, filtrate 4.Get filtrate 1 40 DEG C of rotary evaporations in Rotary Evaporators and extract 2～3h, obtain concentrated solution 1.Get filtrate 2 60 DEG C of rotary evaporations in Rotary Evaporators and extract 1～2h, obtain concentrated solution 2.Get filtrate 3, in Rotary Evaporators, 70 DEG C of rotary evaporations extract 1～2h, obtain concentrated solution 3.Get concentrated solution 1,2,3, after mixing in Rotary Evaporators 80 DEG C of rotary evaporations, until ethanol evaporation is removed, obtain about 2000ml removing heat from blood wound healing formula extraction.Get filtrate 4 and removing heat from blood wound healing formula extraction 60～70 DEG C of rotary evaporation 2h in Rotary Evaporators, the mixture of filtrate 4 and removing heat from blood wound healing formula extraction is taken out from distilling flask, and add 10g Borneolum Syntheticum stirring and dissolving in above-mentioned mixed liquor, obtain about 2000ml removing heat from blood wound healing oil.Get resveratrol 9.12mg and be dissolved in removing heat from blood wound healing oil, obtain compound resveratrol removing heat from blood wound healing oil.Add the about 15ml of azone, the about 30mg of vitamin E is uniformly mixed, and can use again.
Embodiment 3
Take Rhizoma Coptidis 30g, Cortex Phellodendri 30g, Radix Scutellariae 30g, Radix Et Rhizoma Rhei 20g, Radix Sophorae Flavescentis 20g, Cortex Dictamni 20g, Periostracum Cicadae 15g, Endoconcha Sepiae 5g, Radix Arnebiae (Radix Lithospermi) 40g, Radix Sanguisorbae 20g, Flos Carthami 10g, 10 of Pheretimas, 5 grams of Borneolum Syntheticums, medical material is all the other co-grindings except Borneolum Syntheticum, cross 10 molecules of interest orders, add 40% ethanol 2000ml, 80～85 DEG C of reflux, extract, 3h in round-bottomed flask.Then medicinal powder and alcohol mixture are filtered, obtain filtering residue 1, filtrate 1.Get filtering residue 1 and add 40% ethanol 2000ml, 80～85 DEG C of reflux, extract, 2h in round-bottomed flask.Then filtering residue and alcohol mixture are filtered, obtain filtering residue 2, filtrate 2.Get filtering residue 2 and add 40% ethanol 2000ml, 80～85 DEG C of reflux, extract, 1h in round-bottomed flask.Then filtering residue and alcohol mixture are filtered, obtain filtering residue 3, filtrate 3.
Get filtering residue 3 and be placed in natural environment logical dry in the sun wind and dry after 24h, add high mountain Oleum Camelliae 1000ml 90 DEG C of reflux, extract, 3.5h in round-bottomed flask.Then filtering residue and high mountainous tea oil mixture are filtered, obtain filtering residue 4, filtrate 4.Get filtrate 1 40 DEG C of rotary evaporations in Rotary Evaporators and extract 2～3h, obtain concentrated solution 1.Get filtrate 2 60 DEG C of rotary evaporations in Rotary Evaporators and extract 1～2h, obtain concentrated solution 2.Get filtrate 3, in Rotary Evaporators, 70 DEG C of rotary evaporations extract 1～2h, obtain concentrated solution 3.Get concentrated solution 1,2,3, after mixing in Rotary Evaporators 80 DEG C of rotary evaporations, until ethanol evaporation is removed, obtain about 1000ml removing heat from blood wound healing formula extraction.Get filtrate 4 and removing heat from blood wound healing formula extraction 60～70 DEG C of rotary evaporation 2h in Rotary Evaporators, the mixture of filtrate 4 and removing heat from blood wound healing formula extraction is taken out from distilling flask, and add 5g Borneolum Syntheticum stirring and dissolving in above-mentioned mixed liquor, obtain about 1000ml removing heat from blood wound healing oil.Get resveratrol 3.5mg and be dissolved in removing heat from blood wound healing oil, obtain compound resveratrol removing heat from blood wound healing oil.Add the about 4ml of azone, the about 20mg of vitamin E is uniformly mixed, and can use again.
Embodiment 4
Experimental technique
One, the preparation of mice heating power scalding model and administration
30 mices are divided into 3 groups by body weight completely random: negative control group, removing heat from blood wound healing line of oils and compound resveratrol removing heat from blood wound healing line of oils, 10 every group.Slough mice dorsal body setae, the about 7cm of area with 4% sodium sulfide
2.Test the same day, under pentobarbital sodium (30mg/kg) intraperitoneal anesthesia, the square iron plate that area is 2.25 is placed in alcohol burner flame envelope calcination 2min, and by the iron plate contact depilation position, back after calcination, be 4s time of contact, causes approximately dark II degree scalding model.
After scalding 0.5h, smear administration at scald position by group respectively, negative control group gives 0.9% sodium chloride solution, removing heat from blood wound healing line of oils gives removing heat from blood wound healing oil, compound resveratrol removing heat from blood wound healing line of oils gives compound resveratrol removing heat from blood wound healing oil formulation (preparation that embodiment 1 prepares), every day 2 times, continuous 14 days.
Two, the preparation of mice chemical burn model and administration
30 mices are divided into 3 groups by body weight completely random: negative control group, removing heat from blood wound healing line of oils and compound resveratrol removing heat from blood wound healing line of oils, 10 every group.Slough mice dorsal body setae, the about 7cm2 of area with 4% sodium sulfide.Test the same day, under pentobarbital sodium (30mg/kg) intraperitoneal anesthesia, the circular filter paper sheet that is 2cm by the diameter soaking with 2mol/L Naoh is close to depilation position, back, removes filter paper after 4min, uses clear water cleaning down, causes about II degree burn model.
After scalding 0.5h, smear administration respectively at scald position by group, medication is scalded each group with heating power.Cover whole wound surface as degree taking medicinal liquid, every day 2 times, continuous 14 days.
Three, observation index and method
3.1 wound surface gross examination of skeletal muscle observe heating power burned mouses and chemical burn mice after the 1st day, the 3rd day, the 5th day, the 7th day and the 14th day edema wound ooze out degree, whether dry, have all rednesses of noinvasive and secretions etc.
Within after 3.2 wounds in mice area estimation administrations the 1st day, the 3rd day, the 5th day, the 7th day and the 14th day, copy wounds in mice with template, being placed on the length of side is the sub-box paper of 1mm, calculate whole sub-box areas of wound surface scope, with the whole sub-box cartographic represenation of area wound surface areas within the scope of wound surface, reduce degree reflection wound healing situation by wound surface area.
3.3 wounds in mice healing rates are copied wounds in mice with template in the 1st day, the 3rd day, the 5th day, the 7th day and the 14th day after measuring administration, being placed on the length of side is the sub-box paper of 1mm, calculate whole sub-box areas of wound surface scope, the area now calculating is original area, after with same method calculate wound healing cross kind in the area of healing not, and calculate the Wound healing rate of the rear different time phases of wound by following formula: healing rate=(original wound surface area-wound surface area does not heal)/original wound surface area.
3.4 wounds in mice healing times turn to healing standard with the complete epithelium of wound surface, and confirm through wound surface pathological section HE dyeing.
3.5 statistical procedures data represent with x ± s, and each group of data are first carried out to homogeneity test of variance.As homogeneity of variance, carry out one factor analysis of variance with SAS for windows8.1; If heterogeneity of variance, carries out after logarithm conversion, then carry out variance analysis.
Four, result
The present invention has advantages of the combination of Chinese and Western medicine, to treatment burn and scald Chinese medicine oil formulation---removing heat from blood wound healing oil has further improvement.Experimental data shows: first from wound surface gross examination of skeletal muscle result, the inflammatory reaction of compound resveratrol removing heat from blood wound healing line of oils after wound the 7th day all than a little less than negative control group, under the disappearance of wound surface skin turgor, crust, ooze out secretions minimizing and create all rednesses and alleviate, wound inflammation scope is relatively little, healing area is larger, and hair follicle regeneration is obvious.Show that compound resveratrol removing heat from blood wound healing oil has the reagentia of reducing inflammation.Secondly from wound surface area, the wound surface area of chemical burn mice was from the 1st day as can be seen from Table 2, compound resveratrol removing heat from blood wound healing line of oils wound surface area compared with negative control group obviously reduces (p<0.05), and wound surface area reaches 293.3 ± 38.32mm
2.As can be seen here, it is remarkable that early stage therapeutic effect is hindered in the rusting of compound resveratrol removing heat from blood wound healing.From the 3rd day, compound resveratrol removing heat from blood wound healing line of oils wound surface area compared with negative control group all obviously reduces (removing heat from blood wound healing oil p<0.05, compound resveratrol removing heat from blood wound healing oil p<0.01), wound surface area reaches respectively 196.89 ± 143.18mm
2, 184.89 ± 56.31mm
2.As can be seen here, in burn wound healing process, two kinds of medicines all have certain therapeutic effect.And by the 7th day, the relatively wound surface difference in areas opposite sex little (p>0.05) of removing heat from blood wound healing line of oils and negative control group; Compound resveratrol removing heat from blood wound healing line of oils still maintains the level (p<0.01) differing greatly compared with negative control group, and before the 14th day all healed.Therefore can judge that from the dynamic variation of wound surface area treating skin burn at removing heat from blood wound healing oil and compound resveratrol removing heat from blood wound healing oil has and promote preferably skin growth, has good wound healing effect.Compound recipe removing heat from blood wound healing oil has the healing effect of short-term, but stability is bad, its short-term healing effect not obvious (the visible table 1 of result) in the mice of heat injury.Again from healing rate, as can be seen from Table 4, the healing rate of chemical burn mice was from the 3rd day to the 5th day, removing heat from blood wound healing line of oils enlarges markedly (removing heat from blood wound healing oil p<0.05 with compound resveratrol removing heat from blood wound healing line of oils healing rate compared with negative control group, compound resveratrol removing heat from blood wound healing oil p<0.01), reach respectively 0.4857 ± 0.3912,0.3887 ± 0.1628 at the 3rd day healing rate, within the 5th day, healing rate reaches respectively 0.8179 ± 0.09523,0.8098 ± 0.1037.As can be seen here, all there is certain therapeutic effect at the early stage two kinds of medicines of burn treating.And by the 7th day, removing heat from blood wound healing line of oils healing rate diversity little (p>0.05) compared with negative control group; Compound resveratrol removing heat from blood wound healing line of oils still maintains the level (p<0.05) differing greatly compared with negative control group, and healing rate has reached 100% before the 14th day.And in the mice of scalding at heating power, the therapeutic effect of compound recipe removing heat from blood wound healing oil is also the most significantly (the visible table 3 of result), as can be seen here, the therapeutic effect of compound resveratrol removing heat from blood wound healing oil is better.Finally from wound healing time, as can be seen from Table 5, the healing time of heating power burned mouse removing heat from blood wound healing line of oils and compound resveratrol removing heat from blood wound healing line of oils is all than the obvious shortening of negative control group (P<0.05), healing time is respectively 24.6 ± 2.07 days, 22.6 ± 1.67 days, and wherein compound resveratrol removing heat from blood wound healing line of oils healing time shortens more obviously (p<0.01).In sum, the compound resveratrol removing heat from blood wound healing oil formulation that the present invention prepares has the effect that obvious promotion is scalded burned skin wound healing, suppressed scytitis reaction, and effect is better than traditional removing heat from blood wound healing oil.
Table 1 heating power is scalded each group of mice different time skin burn wound surface Area comparison, and (mm2, x s)
Note: compare with negative control group: * P<0.05, * * P<0.01
Mice different time skin burn wound surface Area comparison is respectively organized in table 2 chemical burn, and (mm2, x s)
Note: compare with negative control group: * P<0.05, * * P<0.01, * * * P<0.001
Table 3 heating power is scalded the comparison of each group of mice different time skin burn concrescence rate, and (x s)
Note: compare with negative control group: * P<0.05, * * P<0.01, * * * P<0.001
The comparison of mice different time skin burn concrescence rate is respectively organized in table 4 chemical burn, and (x s)
Note: with negative control comparison: * P<0.05, * * P<0.01, * * * P<0.001
Table 5 heating power is scalded the comparison of each group of mice healing days, and (day, x s)
Note: compare with negative control group: * P<0.05, * * P<0.01
The comparison of mice healing days is respectively organized in table 6 chemical burn, and (day, x s)
Note: compare with negative control group: * P<0.05, * * P<0.01.
Claims (6)
1. a removing heat from blood wound healing formula extraction, is characterized in that, is extracted and is obtained: Rhizoma Coptidis 40g, Cortex Phellodendri 40g, Radix Scutellariae 40g by the raw material that comprises following consumption, Radix Et Rhizoma Rhei 30g, Radix Sophorae Flavescentis 30g, Cortex Dictamni 30g, Periostracum Cicadae 20g, Endoconcha Sepiae 10g, Radix Arnebiae (Radix Lithospermi) 60g, Radix Sanguisorbae 30g, Flos Carthami 20g, 15 of Pheretimas;
Obtain with the ethanol extraction that volume fraction is 40%～80%;
Specifically prepared by the method that comprises following steps:
Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Cortex Dictamni, Periostracum Cicadae, Endoconcha Sepiae, Radix Arnebiae (Radix Lithospermi), Radix Sanguisorbae, Flos Carthami and Pheretima are pressed to above-mentioned weight co-grinding, add volume fraction and be ethanol 2000～4000ml of 40%～80%, controlling temperature is 80～95 DEG C, reflux, extract, 2～4 times, each 1～3h, filtrate is merged, concentrate and get final product.
2. the preparation method of removing heat from blood wound healing formula extraction according to claim 1, is characterized in that, is prepared by the method that comprises following steps:
S1. incite somebody to action: Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Cortex Dictamni, Periostracum Cicadae, Endoconcha Sepiae, Radix Arnebiae (Radix Lithospermi), Radix Sanguisorbae, Flos Carthami and Pheretima are by above-mentioned weight co-grinding, add volume fraction and be ethanol 2000～4000ml of 40%～80%, controlling temperature is 80～85 DEG C, reflux, extract, 1～3h, filter, obtain filtering residue 1 and filtrate 1;
S2. filtering residue 1 being added to volume fraction is ethanol 2000～4000ml of 40%～80%, and controlling temperature is 80～85 DEG C, and reflux, extract, 1～3h filters, and obtains filtering residue 2 and filtrate 2;
S3. filtering residue 2 being added to volume fraction is ethanol 2000～4000ml of 40%～80%, and controlling temperature is 80～85 DEG C, and reflux, extract, 1～2h filters, and obtains filtering residue 3 and filtrate 3;
S4. get filtrate 1 40 DEG C of rotary evaporations in Rotary Evaporators and extract 2～3h, obtain concentrated solution 1;
S5. get filtrate 2 60 DEG C of rotary evaporations in Rotary Evaporators and extract 1～2h, obtain concentrated solution 2;
S6. get filtrate 3, in Rotary Evaporators, 70 DEG C of rotary evaporations extract 1～2h, obtain concentrated solution 3;
S7. get concentrated solution 1,2,3, after mixing in Rotary Evaporators 80 DEG C of rotary evaporations, until ethanol evaporation is complete, to obtain final product.
3. the preparation method of removing heat from blood wound healing formula extraction according to claim 2, is characterized in that, is prepared by the method that comprises following steps:
S1. incite somebody to action: Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Cortex Dictamni, Periostracum Cicadae, Endoconcha Sepiae, Radix Arnebiae (Radix Lithospermi), Radix Sanguisorbae, Flos Carthami and Pheretima are by above-mentioned weight co-grinding, add volume fraction and be 60% ethanol 3000ml, controlling temperature is 80～85 DEG C, reflux, extract, 1～3h, filter, obtain filtering residue 1 and filtrate 1;
S2. filtering residue 1 being added to volume fraction is 60% ethanol 3000ml, and controlling temperature is 80～85 DEG C, and reflux, extract, 2h filters, and obtains filtering residue 2 and filtrate 2;
S3. filtering residue 2 being added to volume fraction is 60% ethanol 3000ml, and controlling temperature is 80～85 DEG C, and reflux, extract, 1h filters, and obtains filtering residue 3 and filtrate 3;
S4. get filtrate 1 40 DEG C of rotary evaporations in Rotary Evaporators and extract 2～3h, obtain concentrated solution 1;
S5. get filtrate 2 60 DEG C of rotary evaporations in Rotary Evaporators and extract 1～2h, obtain concentrated solution 2;
S6. get filtrate 3, in Rotary Evaporators, 70 DEG C of rotary evaporations extract 1～2h, obtain concentrated solution 3;
S7. get concentrated solution 1,2,3, after mixing in Rotary Evaporators 80 DEG C of rotary evaporations, until ethanol evaporation is complete, to obtain final product.
4. a compound resveratrol removing heat from blood wound healing oil formulation for the treatment of skin burn, is characterized in that, comprises following component: removing heat from blood wound healing formula extraction 500～1000ml; High mountain Oleum Camelliae 1000～2000ml; Borneolum Syntheticum 5～10g; Resveratrol 3.04～9.12mg; Penetrating agent: 4～20ml; Antiseptic: 1～30ml;
Described removing heat from blood wound healing formula extraction is extracted and is obtained by the raw material that comprises following consumption: Rhizoma Coptidis 40g, Cortex Phellodendri 40g, Radix Scutellariae 40g, Radix Et Rhizoma Rhei 30g, Radix Sophorae Flavescentis 30g, Cortex Dictamni 30g, Periostracum Cicadae 20g, Endoconcha Sepiae 10g, Radix Arnebiae (Radix Lithospermi) 60g, Radix Sanguisorbae 30g, Flos Carthami 20g, 15 extractions of Pheretima make;
Described penetrating agent is azone, and antiseptic is vitamin E.
5. a compound resveratrol removing heat from blood wound healing oil formulation for the treatment of skin burn, is characterized in that, comprises following component: removing heat from blood wound healing formula extraction 500～1000ml; High mountain Oleum Camelliae 1000～2000ml; Borneolum Syntheticum 5～10g; Resveratrol 3.04～9.12mg; Penetrating agent: 4～20ml; Antiseptic: 1～30ml;
Described removing heat from blood wound healing formula extraction is extracted and is obtained by the raw material that comprises following consumption: Rhizoma Coptidis 40g, Cortex Phellodendri 40g, Radix Scutellariae 40g, Radix Et Rhizoma Rhei 30g, Radix Sophorae Flavescentis 30g, Cortex Dictamni 30g, Periostracum Cicadae 20g, Endoconcha Sepiae 10g, Radix Arnebiae (Radix Lithospermi) 60g, Radix Sanguisorbae 30g, Flos Carthami 20g, 15 extractions of Pheretima make;
Described penetrating agent is azone, and antiseptic is vitamin E;
Specifically comprise the following steps:
S1. after the filtering residue 3 after claim 3 being extracted is dried, mix with 1000～2000ml high mountain Oleum Camelliae, controlling temperature is 85～90 DEG C, and reflux, extract, 2～4h filters, and obtains filtrate 4;
S2. get filtrate 4 and removing heat from blood wound healing formula extraction 60～70 DEG C of rotary evaporation 2h in Rotary Evaporators, add Borneolum Syntheticum stirring and dissolving in above-mentioned mixed liquor, obtain removing heat from blood wound healing oil;
S3. get resveratrol and be dissolved in removing heat from blood wound healing oil, obtain compound resveratrol removing heat from blood wound healing oil; Add penetrating agent, antiseptic is uniformly mixed again, and to obtain final product.
6. the preparation method of compound resveratrol removing heat from blood wound healing oil formulation claimed in claim 4, is characterized in that, comprises the steps:
S1. after the filtering residue 3 after claim 3 being extracted is dried, mix with 1000～2000ml high mountain Oleum Camelliae, controlling temperature is 85～90 DEG C, and reflux, extract, 2～4h filters, and obtains filtrate 4;
S2. get filtrate 4 and removing heat from blood wound healing formula extraction 60～70 DEG C of rotary evaporation 2h in Rotary Evaporators, add Borneolum Syntheticum stirring and dissolving in above-mentioned mixed liquor, obtain removing heat from blood wound healing oil;
S3. get resveratrol and be dissolved in removing heat from blood wound healing oil, obtain compound resveratrol removing heat from blood wound healing oil; Add penetrating agent, antiseptic is uniformly mixed again, and to obtain final product.
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| US9980924B2 (en) * | 2014-09-04 | 2018-05-29 | The Regents Of The University Of California | Topical pterostilbene compositions for use in treating UV-induced loss of barrier function in skin |
| CN110123841A (en) * | 2018-02-09 | 2019-08-16 | 上海市第六人民医院 | Load purposes of the human pluripotent stem cells excretion body in preparation treatment genital system diseases drug of resveratrol |
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