CN104961538A - Pepper continuous cropping obstacle improvement attapulgite-based immobilized complex microbial agent and preparation method thereof - Google Patents
Pepper continuous cropping obstacle improvement attapulgite-based immobilized complex microbial agent and preparation method thereof Download PDFInfo
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- CN104961538A CN104961538A CN201510228810.7A CN201510228810A CN104961538A CN 104961538 A CN104961538 A CN 104961538A CN 201510228810 A CN201510228810 A CN 201510228810A CN 104961538 A CN104961538 A CN 104961538A
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- continuous cropping
- recessed soil
- recessed
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- organism fungicide
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Abstract
The present invention discloses a pepper continuous cropping obstacle improvement attapulgite-based immobilized complex microbial agent which is characterized in that: the pepper continuous cropping obstacle improvement attapulgite-based immobilized complex microbial agent comprises the following ingredients by mass: 35 to 50 % of attapulgite, 15 to 25 % of briquette slag, 15 to 25% % of decomposed manure, 5 to 15% of humic acid and 5 to 10% of a complex microbial agent. The purpose of the present invention to provide the pepper continuous cropping obstacle improvement attapulgite-based immobilized complex microbial agent and a preparation method thereof, the interaction between different strains of Bacillus is fully used, the pepper continuous cropping obstacle improvement attapulgite-based immobilized complex microbial agent has the effects of stimulating plant growth, alleviating land compaction, improving soil ecological environment, preventing and resisting diseases resistance, and the like, and can effectively ease formation and aggravation problems of pepper continuous cropping obstacles.
Description
Technical field
The present invention relates to technical field of crop cultivation, be specifically related to a kind of for recessed soil matrix immobilization complex micro organism fungicide improveing capsicum continuous cropping obstacle and preparation method thereof.
Background technology
Continuous cropping obstacle has become the bottleneck of restriction capsicum industry Sustainable development.The Auto toxicity that under sequential cropping cultivation condition, soil nutrient is unbalanced, the accumulation of soil physical and chemical property deterioration, soil surface characters, root-zone soil functional disorder and allelochemical etc. cause is all the major cause causing yield of hot pepper and quality to decline.But due to continuous cropping obstacle produce factor very complicated, production still lacks a set of effective solution or approach.The technology overcoming continuous cropping obstacle conventional in current production has crop rotation and intercropping and interplanting, select disease-resistant variety or grafting, soilless culture, reasonably soil management and biological control etc.Wherein utilize microbial preparation to improve soil, degraded chemical residual, improve the ecological environment, promote crop yield, bacteriostatic and disease prevention etc., become the focus of research of agricultural science.
Summary of the invention
The object of the invention is to provide a kind of for recessed soil matrix immobilization complex micro organism fungicide improveing capsicum continuous cropping obstacle and preparation method thereof, make full use of the interaction between different Bacillus, play stimulating plant growth, alleviate the hardening soil, improve the effect such as ecological environment of soil, disease resistance, effectively alleviate formation and the penalty issue of capsicum continuous cropping obstacle.
The present invention is achieved through the following technical solutions:
A kind of recessed soil matrix immobilization complex micro organism fungicide for improveing capsicum continuous cropping obstacle, it is characterized in that: described recessed soil matrix immobilization complex micro organism fungicide is made up of according to mass percent following component: recessed native 35 ~ 50%, Hongcomb coal-slag 15 ~ 25%, decomposed cow dung 15 ~ 25%, humic acid 5 ~ 15%, composite fungus agent 5 ~ 10%.
The Technological improvement plan that the present invention solves further is:
Described composite fungus agent is made up of subtilis, bacillus laterosporus, Bacillus licheniformis, subtilis, bacillus laterosporus, Bacillus licheniformis are fermented respectively, be about 3: 2: 1 in viable bacteria ratio and make mixed fermentation liquid, viable count is greater than 1.0 × 10
10cfu/mL.
The Technological improvement plan that the present invention solves further is:
In described recessed soil matrix immobilization complex micro organism fungicide, total viable count is greater than 10.0 × 10
8cfu/g.
The present invention is achieved through the following technical solutions:
Prepare the method for recessed soil matrix immobilization complex micro organism fungicide, it is characterized in that: comprise the following steps:
1) choose the recessed soil of purifying that particle diameter is about 1mm, and carry out sterilising treatment;
2) according to above-mentioned component mass percent scope, selected one group of recessed soil through step 1 process and composite fungus agent proportioning, being added by recessed soil is in the composite bacteria fermentation liquid of logarithmic phase, mixed fermentation 12 ~ 24h, make microorganism adsorption biofilm, drying, obtained recessed native immobilization complex microorganism;
3) Hongcomb coal-slag, decomposed cow dung and humic acid are pulverized respectively in pulverizer, size controlling at 1 ~ 2mm, and carries out sterilising treatment;
4) by the recessed native immobilization complex microorganism that step 2 is obtained, obtained with step 3 by mass percentage Hongcomb coal-slag, decomposed cow dung and humic acid particle mix, drying, obtained recessed soil matrix immobilization complex micro organism fungicide.
The present invention compared with prior art, has following obvious advantage:
1, the present invention selects subtilis, bacillus laterosporus, Bacillus licheniformis is as the composite raw material of complex micro organism fungicide, it is antibacterial that subtilis has diseases prevention, strengthen the effect of plant disease-resistant performance, Bacillus licheniformis can produce multiple protein enzyme and antagonistic substance and phytokinin and dormin etc., bacillus laterosporus can produce desinsection, Substance, there is phosphorus decomposing, the several functions such as growth-promoting, also there is the ability of stronger opposing ambient pressure environment, can resist in living environment due to drying, the injury that heat and ultraviolet radiation cause, maintenance self-ability is unaffected.Visible, genus bacillus contains antibacterium, antiviral material, the virulence of these substance passivation pathogenic agent and suppression pathogenic growth, improve edaphon and crop to the absorption of soil nutrient, and the inverse gemma of heat-resistant can be produced, be beneficial to the production of biocontrol fungicide, formulation and survive in the environment, surely grow and breeding, the collocation of mentioned microorganism kind rationally, by force functional, can effectively degrade chemical residual, improve ecological environment of soil, bacteriostatic and disease prevention and improvement soil, to alleviation capsicum continuous cropping obstacle successful.
2, the present invention utilizes recessed soil as fixation of microbe carrier, recessed soil is a kind of natural rich zeopan clay mineral with good adsorbent performance and colloidal property, it is the friendly carrier carrying microorganism, the nutritive element in soil can also be adsorbed, increase soil aggregate, keep and regulate soil fertility, regulating pH value, Promoting plant growth; Hongcomb coal-slag can improve the water-permeable and air permeable of soil to a certain extent, and the inorganic salt contained in cinder also can provide some nutrients for plant-growth, and Hongcomb coal-slag, as main raw material, improves the utilization ratio of resource, turns waste into wealth; Cow dung and humic acid have stronger ion-exchange capacity and adsorptive power, have complexing and shock absorption, and adding humic acid can increase soil fertility, and improves soil physico-chemical property.
3, the present invention's recessed soil matrix immobilization complex micro organism fungicide is equally applicable to other crops.
Embodiment
The present invention's recessed soil matrix immobilization complex micro organism fungicide is made up of according to mass percent following component: recessed native 35 ~ 50%, Hongcomb coal-slag 15 ~ 25%, decomposed cow dung 15 ~ 25%, humic acid 5 ~ 15%, composite fungus agent 5 ~ 10%; Described composite fungus agent is numbered CICC23767 by Bacillus subtilis strain, bacillus laterosporus bacterium numbering is CICC23376, Bacillus licheniformis strain is numbered CICC23714 and forms, subtilis, bacillus laterosporus, Bacillus licheniformis are fermented respectively, be about 3: 2: 1 in viable bacteria ratio and make mixed fermentation liquid, viable count is greater than 1.0 × 10
10cfu/mL; In recessed soil matrix immobilization complex micro organism fungicide, total viable count is greater than 10.0 × 10
8cfu/g.
Prepare the method for recessed soil matrix immobilization complex micro organism fungicide, comprise the following steps:
1) choose the recessed soil of purifying that particle diameter is about 1mm, and carry out sterilising treatment;
2) according to above-mentioned component mass percent scope, selected one group of recessed soil through step 1 process and composite fungus agent proportioning, being added by recessed soil is in the composite bacteria fermentation liquid of logarithmic phase, mixed fermentation 12 ~ 24h, make microorganism adsorption biofilm, drying, obtained recessed native immobilization complex microorganism;
3) Hongcomb coal-slag, decomposed cow dung and humic acid are pulverized respectively in pulverizer, size controlling at 1 ~ 2mm, and carries out sterilising treatment;
4) by the recessed native immobilization complex microorganism that step 2 is obtained, obtained with step 3 by mass percentage Hongcomb coal-slag, decomposed cow dung and humic acid particle mix, drying, obtained recessed soil matrix immobilization complex micro organism fungicide.
In order to deepen the understanding of the present invention and understanding, further illustrate technical solution of the present invention below in conjunction with embodiment, these embodiments should not be understood as the restriction to technical solution.
embodiment 1:
1) respectively slant culture, shaking table cultivation and fermentation culture are carried out to subtilis, bacillus laterosporus, Bacillus licheniformis, be about 3: 2: 1 in viable bacteria ratio and make mixed fermentation liquid;
2) by recessed soil and composite fungus agent 5:1 mixed fermentation 12 ~ 24h, microorganism adsorption biofilm is made, dry, obtain recessed native immobilization complex microorganism;
3) by particle diameter be the Hongcomb coal-slag of 1mm, decomposed cow dung and humic acid powder, sterilizing;
4) mixed according to 50:20:25:5 with Hongcomb coal-slag, decomposed cow dung and humic acid by recessed native immobilized microorganism, dry, total viable count is greater than 10.0 × 10
8cfu/g.
embodiment 2:
1) respectively slant culture, shaking table cultivation and fermentation culture are carried out to subtilis, bacillus laterosporus, Bacillus licheniformis, be about 3: 2: 1 in viable bacteria ratio and make mixed fermentation liquid;
2) by recessed soil and composite fungus agent 8:1 mixed fermentation 12 ~ 24h, microorganism adsorption biofilm is made, dry, obtain recessed native immobilization complex microorganism;
3) by particle diameter be the Hongcomb coal-slag of 1mm, decomposed cow dung and humic acid powder, sterilizing;
4) recessed native immobilized microorganism is mixed according to 45:15:25:15 with Hongcomb coal-slag, decomposed cow dung and humic acid, dry, obtain complex micro organism fungicide, total viable count is greater than 10.0 × 10
8cfu/g.
recessed soil matrix immobilization complex micro organism fungicide affects Application Example to continuous cropping chili growth and output:
1 materials and methods
1.1 material
Capsicum variety is ' good agriculture 11 '.
1.2 test design
Test the yellow code hot red pepper production base in Qingpu District, Huai'an to carry out, greenhouse gardening capsicum year is limited to 10 years.Test adopts randomized block design, and recessed soil matrix immobilization complex micro organism fungicide above-described embodiment 1 and embodiment 2 obtained and fertilizer mixing are manured into soil, and Rate of Organic Fertilizer is 500kg ∕ 667m
2, two kinds of complex micro organism fungicide consumptions are 15kg ∕ 667m
2, to apply fertilizer for contrast, often process repetition 3 times, totally 9 communities, each plot area 4m × 6m, seeding row spacing 40cm × 50cm, if protection row 2 row.Booth Routine Management.
Investigate the plant height of pepper plant, root system volume, amount of dry matter and pulp thickness collection period, statistics yield of hot pepper, and measure chlorophyll content and improving activity of root system.
2 results and analysis
2.1 microbial inoculums are on the impact of continuous cropping Folium Capsici chlorophyll contents and improving activity of root system
As can be seen from Table 1, after using recessed soil matrix immobilization complex micro organism fungicide, Folium Capsici chlorophyll contents comparatively CK significantly increases, and improving activity of root system also obviously strengthens, and illustrates that recessed soil matrix immobilization complex micro organism fungicide can improve photosynthetic capacity and the Root Absorption ability of capsicum.
Table 1 microbial inoculum is on the impact of continuous cropping chili growth, output and physical signs
2.2 microbial inoculums are on the impact of continuous cropping chili growth and output
After using recessed soil matrix immobilization complex micro organism fungicide, chili growth gesture is significantly better than contrast, and vigorous period extends, and leaf area increases, and blade is thickening, and leaf look turns green.As can be seen from Table 1, the plant height of T1 and T2 comparatively contrasts increase by 15.75% and 14.58%, and root system volume increases by 28.74% and 24.63%, dry-matter increases by 31.32% and 30.93%, pulp is obviously thickening, and fruit yield also significantly increases, and amplification is respectively 23.86% and 21.70%.
3 conclusions
After using recessed soil matrix immobilization complex micro organism fungicide, capsicum root system improves to nutrient uptake and utilization ability, and the photosynthetic Utilization ability of plant significantly improves, and the plant strain growth phase extends, and bearing capacity strengthens; Recessed soil matrix immobilization complex micro organism fungicide can not only significantly promote continuous cropping chili growth, and dry-matter accumulation is increased, and can also significantly promote that capsicum is increased production, and has obvious effect to overcoming capsicum continuous cropping obstacle.
It should be noted that above-described embodiment is only preferred embodiment of the present invention; be not used for limiting protection scope of the present invention; the equivalent replacement that the basis of technique scheme is made or alternative; all belong to protection scope of the present invention, protection scope of the present invention is as the criterion with claims.
Claims (4)
1. one kind for improveing the recessed soil matrix immobilization complex micro organism fungicide of capsicum continuous cropping obstacle, it is characterized in that: described recessed soil matrix immobilization complex micro organism fungicide is made up of according to mass percent following component: recessed native 35 ~ 50%, Hongcomb coal-slag 15 ~ 25%, decomposed cow dung 15 ~ 25%, humic acid 5 ~ 15%, composite fungus agent 5 ~ 10%.
2. a kind of recessed soil matrix immobilization complex micro organism fungicide for improveing capsicum continuous cropping obstacle according to claim 1, it is characterized in that: described composite fungus agent is made up of subtilis, bacillus laterosporus, Bacillus licheniformis, subtilis, bacillus laterosporus, Bacillus licheniformis are fermented respectively, be about 3: 2: 1 in viable bacteria ratio and make mixed fermentation liquid, viable count is greater than 1.0 × 10
10cfu/mL.
3. a kind of recessed soil matrix immobilization complex micro organism fungicide for improveing capsicum continuous cropping obstacle according to claim 1 and 2, is characterized in that: in described recessed soil matrix immobilization complex micro organism fungicide, total viable count is greater than 10.0 × 10
8cfu/g.
4. preparing the method for a kind of recessed soil matrix immobilization complex micro organism fungicide for improveing capsicum continuous cropping obstacle as claimed in claim 1, it is characterized in that: comprise the following steps:
1) choose the recessed soil of purifying that particle diameter is about 1mm, and carry out sterilising treatment;
2) according to above-mentioned component mass percent scope, selected one group of recessed soil through step 1 process and composite fungus agent proportioning, being added by recessed soil is in the composite bacteria fermentation liquid of logarithmic phase, mixed fermentation 12 ~ 24h, make microorganism adsorption biofilm, drying, obtained recessed native immobilization complex microorganism;
3) Hongcomb coal-slag, decomposed cow dung and humic acid are pulverized respectively in pulverizer, size controlling at 1 ~ 2mm, and carries out sterilising treatment;
4) by the recessed native immobilization complex microorganism that step 2 is obtained, obtained with step 3 by mass percentage Hongcomb coal-slag, decomposed cow dung and humic acid particle mix, drying, obtained recessed soil matrix immobilization complex micro organism fungicide.
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Cited By (7)
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| CN105801240A (en) * | 2016-01-29 | 2016-07-27 | 山东鑫圣源生物工程技术有限公司 | Fertilizer batching microbial agent and preparation method therefor |
| CN106008101A (en) * | 2016-05-25 | 2016-10-12 | 山西凯盛肥业集团有限公司 | Composite microbial organic fertilizer for saline-alkali soil improvement and preparation method thereof |
| CN106085919A (en) * | 2016-08-03 | 2016-11-09 | 界首市沃土生物科技有限公司 | A kind of microbial bacterial agent improving Fructus Capsici continuous cropping obstacle |
| CN109438111A (en) * | 2018-11-15 | 2019-03-08 | 贵州品红农业开发有限公司 | A kind of soil conditioner and preparation method thereof on pepper planting ground |
| CN109729940A (en) * | 2019-03-20 | 2019-05-10 | 罗希榕 | Evaluating different microorganisms microbial inoculum influences the development of continuous cropping chili growth, yield, quality, the method for pest and disease damage and Microorganism Characteristics |
| CN112602561A (en) * | 2020-12-11 | 2021-04-06 | 广东博沃特生物技术有限公司 | Growth-promoting type culture medium and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105272621A (en) * | 2015-11-02 | 2016-01-27 | 中国科学院寒区旱区环境与工程研究所 | Preparation and application of composite bioactive material taking attapulgite to load bacillus |
| CN105801240A (en) * | 2016-01-29 | 2016-07-27 | 山东鑫圣源生物工程技术有限公司 | Fertilizer batching microbial agent and preparation method therefor |
| CN106008101A (en) * | 2016-05-25 | 2016-10-12 | 山西凯盛肥业集团有限公司 | Composite microbial organic fertilizer for saline-alkali soil improvement and preparation method thereof |
| CN106085919A (en) * | 2016-08-03 | 2016-11-09 | 界首市沃土生物科技有限公司 | A kind of microbial bacterial agent improving Fructus Capsici continuous cropping obstacle |
| CN109438111A (en) * | 2018-11-15 | 2019-03-08 | 贵州品红农业开发有限公司 | A kind of soil conditioner and preparation method thereof on pepper planting ground |
| CN109729940A (en) * | 2019-03-20 | 2019-05-10 | 罗希榕 | Evaluating different microorganisms microbial inoculum influences the development of continuous cropping chili growth, yield, quality, the method for pest and disease damage and Microorganism Characteristics |
| CN112602561A (en) * | 2020-12-11 | 2021-04-06 | 广东博沃特生物技术有限公司 | Growth-promoting type culture medium and preparation method and application thereof |
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| CN104961538B (en) | 2017-11-14 |
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