CN104958366A - Veterinary traditional Chinese medicine and probiotics compound preparation and method for manufacturing same - Google Patents
Veterinary traditional Chinese medicine and probiotics compound preparation and method for manufacturing same Download PDFInfo
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- CN104958366A CN104958366A CN201510333345.3A CN201510333345A CN104958366A CN 104958366 A CN104958366 A CN 104958366A CN 201510333345 A CN201510333345 A CN 201510333345A CN 104958366 A CN104958366 A CN 104958366A
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Abstract
The invention discloses a veterinary traditional Chinese medicine and probiotics compound preparation and a method for manufacturing the same. The veterinary traditional Chinese medicine and probiotics compound preparation which is a traditional Chinese medicine composition can be oral liquid. The method includes manufacturing steps of (1), smashing traditional Chinese medicine until the size of the traditional Chinese medicine reaches 60-80 meshes, weighing the traditional Chinese medicine according to formula ratios and three-dimensionally mixing the traditional Chinese medicine to obtain traditional Chinese medicine powder; (2), respectively feeding the mixed traditional Chinese medicine powder and 300-600 parts of water into an extracting tank and soaking the mixed traditional Chinese medicine powder for 5-20 hours; (3), heating the traditional Chinese medicine powder and the water until the temperatures of the traditional Chinese medicine powder and the water reach 40-100 DEG C and extracting the traditional Chinese medicine powder for 10-15 hours; (4), filtering and collecting medicine liquid; (5), adding 300-600 parts of water into remained medicine residues, and extracting the remained medicine residues for 10-15 hours at the temperature of 40-100 DEG C; (6), filtering and removing the medicine residues to obtain extract, combining the extract with the medicine liquid to obtain mixed liquid and concentrating the mixed liquid at the temperature of 30-70 DEG C under reduced pressures until the volume of concentrated mixed liquid is 10-20% of the original volume of the mixed liquid. The veterinary traditional Chinese medicine and probiotics compound preparation and the method have the advantages that the intestinal tracts of animals can be improved while viral diseases such as influenza can be treated, complications such as diarrhea can be prevented and treated, the nutrient absorption can be enhanced, and the organism immunity of the animals can be improved.
Description
Technical field
The present invention relates to a kind of veterinary Chinese medicine and probiotics composite preparation and preparation method thereof.
Background technology
Lot of experiments shows, has the effect of dynamic adjustments animal gastrointestinal tract flora ecosystem with probiotic bacteria microbial inoculum.There is promotion animal growth, improve food conversion ratio, improve animal products quality, strengthen animal immunizing power, improve Anti-bacterium, antiviral ability.But, owing to adding antibiotic for a long time in feedstuff, then severe, seriously polluted due to breeding environment, be only also not enough to accomplish to make cultivated animals to resist pathogenic bacteria, the infecting of virus completely by probiotic bacteria.The bird flu of annual plant is popular, and it is exactly best clear proof that viral diarrhea is difficult to cure.Many Chinese medicine research data show, Chinese medicine also has prevention, effect such as treatment viral influenza, viral diarrhea etc., also has simultaneously and promotes animal growth, enhancing Immune Function In Animals.The immunity of animal will be improved further after the two compound, improves pre-anti-bacteria, the ability of upper respiratory tract infection that virus causes, intestinal tract disease.And then stop using antibiotic completely at livestock breeding industry, replace antibiotic diseases prevention with the compound formulation of probiotic bacteria and Chinese medicine, cure the disease, the products such as egg, milk, meat no longer include antibiotic remains, residue of veterinary drug, and fertility performance improves, quality improves, environmental benefit and ecological benefits promote, and really realize the ecological cultivation of livestock breeding industry.
Summary of the invention
Technical problem to be solved by this invention is: provide a kind of veterinary Chinese medicine and probiotics composite preparation and preparation method thereof.
The present invention solves this technical problem adopted technical scheme: Chinese medicine extraction liquid and probiotics fermention liquid formulated according to volume ratio 1:1.The wherein mixed bacterial that is made up of bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, Lactobacillus buchneri of probiotics fermention liquid, wherein the percentage by weight of all kinds of bacterium is: Lactobacillus plantarum 30-50%, Rhodopseudomonas palustris 10-30%, Lactobacillus plantarum 20-40%, Lactobacillus buchneri 20-40%.The preparation method of Tiny ecosystem probiotics bacterial preparation comprises the steps: activated spawn, level liquid amplification culture, secondary liquid amplification culture, is immobilized onto in rice chaff: dry, pulverizing: bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, Lactobacillus buchneri are mixed according to the following ratio: Lactobacillus plantarum 30-50%, Rhodopseudomonas palustris 10-30%, Lactobacillus plantarum 20-40%, Lactobacillus buchneri 20-40%.Chinese medicine extraction liquid is prepared from by by the Radix Astragali, Fructus Forsythiae, Radix Isatidis, Flos Lonicerae.This Chinese medicine composition can be oral liquid, and its preparation process is: Chinese medicine powder is broken to 60-80 order by (1), takes by formula ratio, and three-dimensional hybrid obtains Chinese medicine powder; (2) mixed Chinese medicine powder and 300-600 part water are put in extraction pot respectively, soak 5-20 hour; (3) be heated to 40-100 DEG C and extract 10-15 hour; (4) collecting by filtration medicinal liquid; (5) remaining medicinal residues continue to add 300-600 part water, and 40-100 DEG C is extracted 10-15 hour; (6) excessively filter medicinal residues, merge extracted twice liquid, 30-70 DEG C is evaporated to original volume 10%-20%.
Beneficial effect of the present invention:
1. Chinese medicine is matched with probiotic bacteria, can play and control foster synchronous object, while the viral diseases such as treatment influenza, improve animal body immunity.
2. can replace antibiotic and chemical disease-resistant drug within the specific limits, reach real nonreactive green cultivation.
Detailed description of the invention
EXAMPLE l
1, the preparation of probiotics fermention liquid:
1) activation each slant strains of preserving, respectively shake-flask culture, bacillus subtilis, Rhodopseudomonas palustris LB culture medium, pH7.3,120 DEG C of autoclavings 30 minutes, cultivate 15 hours at 37 DEG C.Lactobacillus plantarum, Lactobacillus buchneri MRS culture medium, pH6.5,120 DEG C of autoclavings 30 minutes, cultivate 20 hours at 30 DEG C;
2) the level liquid amplification culture (50L tank) of bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, Lactobacillus buchneri; Inoculum concentration 15%;
3) the secondary liquid amplification culture (500L tank) of bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, Lactobacillus buchneri; Inoculum concentration 15%;
4) bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, Lactobacillus buchneri fermentation liquid are mixed according to the following ratio: Lactobacillus plantarum 40%, Rhodopseudomonas palustris 20%, Lactobacillus plantarum 20%, Lactobacillus buchneri 20%;
2, Chinese medicine extraction liquid and preparation method thereof
1) Chinese medicine powder is broken to 80 orders, takes by formula ratio, three-dimensional hybrid obtains Chinese medicine powder;
2) mixed Chinese medicine powder, 500 parts of water are put in extraction pot respectively, soak 8 hours; (3) be heated to 100 DEG C extract 12 hours;
3) collecting by filtration extracting solution;
4) secondary adds 500 parts of water in medicinal residues, and 100 DEG C are extracted 12 hours;
5) merge extracted twice liquid, 60 DEG C are evaporated to original volume 10%;
3, Chinese medicine extraction liquid is mixed according to volume ratio 1:1 with probiotics fermention liquid.
Claims (5)
1. a veterinary Chinese medicine and probiotics composite preparation and preparation method thereof.Said preparation mainly by Chinese medicine extraction liquid and probiotics fermention liquid formulated according to volume ratio 1:1.
2. the wherein mixed bacterial that is made up of bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, Lactobacillus buchneri of probiotics fermention liquid, wherein the percentage by weight of all kinds of bacterium is: Lactobacillus plantarum 30-50%, Rhodopseudomonas palustris 10-30%, Lactobacillus plantarum 20-40%, Lactobacillus buchneri 20-40%.
3. Chinese medicine extraction liquid is prepared from by by the Radix Astragali, Fructus Forsythiae, Radix Isatidis, Flos Lonicerae.Its mass fraction is as follows: Radix Astragali 20-40 part, Fructus Forsythiae 20-40 part, Radix Isatidis 20-40 part, Flos Lonicerae 20-40 part.
4. the preparation method of probiotics fermention liquid, comprises the steps: to activate each slant strains of preserving, respectively shake-flask culture, bacillus subtilis, Rhodopseudomonas palustris LB culture medium, pH7-7.5,100-130 DEG C of autoclaving 20-40 minute, cultivates 12-20 hour at 30-38 DEG C.Lactobacillus plantarum, Lactobacillus buchneri MRS culture medium, pH6-7,100-130 DEG C of autoclaving 20-40 minute, cultivates 15-25 hour at 25-35 DEG C; The level liquid amplification culture (50L tank) of bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, Lactobacillus buchneri; Inoculum concentration 10%-20%; The secondary liquid amplification culture (500L tank) of bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, Lactobacillus buchneri; Inoculum concentration 10%-20%; Bacillus subtilis, Rhodopseudomonas palustris, Lactobacillus plantarum, Lactobacillus buchneri fermentation liquid are mixed according to the following ratio: Lactobacillus plantarum 30-50%, Rhodopseudomonas palustris 10-30%, Lactobacillus plantarum 20-40%, Lactobacillus buchneri 20-40%.
5. Chinese medicine extraction liquid and preparation method thereof is as follows: Chinese medicine powder is broken to 60-80 order by (1), takes by formula ratio, and three-dimensional hybrid obtains Chinese medicine powder; (2) mixed Chinese medicine powder, 300-600 part water are put in extraction pot respectively, soak 5-20 hour; (3) be heated to 40-100 DEG C and extract 10-15 hour; (4) collecting by filtration extracting solution; (5) secondary adds 300-600 part water in medicinal residues, and 40-100 DEG C is extracted 10-15 hour; (6) merge extracted twice liquid, 30-70 DEG C is evaporated to original volume 10%-20%.
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| CN201510333345.3A CN104958366A (en) | 2015-06-16 | 2015-06-16 | Veterinary traditional Chinese medicine and probiotics compound preparation and method for manufacturing same |
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| CN201510333345.3A CN104958366A (en) | 2015-06-16 | 2015-06-16 | Veterinary traditional Chinese medicine and probiotics compound preparation and method for manufacturing same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135712A (en) * | 2016-07-05 | 2016-11-23 | 青岛农业大学 | Mink specific complex probiotics fermention herbal health care oral liquid and preparation method |
| CN106955301A (en) * | 2017-04-17 | 2017-07-18 | 哈尔滨生物制品二厂有限责任公司 | A kind of Traditional Chinese medicine probiotic preparation and preparation method thereof |
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| CN102219309A (en) * | 2011-03-13 | 2011-10-19 | 沧州市中信生物科技有限公司 | SWater purifying agent with quick result |
| CN102813949A (en) * | 2012-08-27 | 2012-12-12 | 宜宾市万华生物环保有限公司 | Microbial environment protectant |
| CN103060247A (en) * | 2013-01-25 | 2013-04-24 | 赵颖 | Probiotics microbial agent for regulating livestock intestinal flora and preparation method thereof |
| CN103655684A (en) * | 2012-09-17 | 2014-03-26 | 蒋盛军 | Traditional Chinese herbal medicine microecological preparation capable of killing and inhibiting foot and mouth disease virus in vitro and in vivo |
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2015
- 2015-06-16 CN CN201510333345.3A patent/CN104958366A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102219309A (en) * | 2011-03-13 | 2011-10-19 | 沧州市中信生物科技有限公司 | SWater purifying agent with quick result |
| CN102813949A (en) * | 2012-08-27 | 2012-12-12 | 宜宾市万华生物环保有限公司 | Microbial environment protectant |
| CN103655684A (en) * | 2012-09-17 | 2014-03-26 | 蒋盛军 | Traditional Chinese herbal medicine microecological preparation capable of killing and inhibiting foot and mouth disease virus in vitro and in vivo |
| CN103060247A (en) * | 2013-01-25 | 2013-04-24 | 赵颖 | Probiotics microbial agent for regulating livestock intestinal flora and preparation method thereof |
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| 胡元亮: "《新编中兽医验方与妙用》", 31 March 2009, 化学工业出版社 * |
| 钱存忠: "《兽药速查手册》", 30 June 2014, 中国农业科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135712A (en) * | 2016-07-05 | 2016-11-23 | 青岛农业大学 | Mink specific complex probiotics fermention herbal health care oral liquid and preparation method |
| CN106955301A (en) * | 2017-04-17 | 2017-07-18 | 哈尔滨生物制品二厂有限责任公司 | A kind of Traditional Chinese medicine probiotic preparation and preparation method thereof |
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