CN104946699B - A kind of biliquid phase fermentation method for being coupled situ extracting fermentation monascus yellow pigment - Google Patents
A kind of biliquid phase fermentation method for being coupled situ extracting fermentation monascus yellow pigment Download PDFInfo
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Abstract
The invention discloses a kind of biliquid phase fermentation method for being coupled situ extracting fermentation monascus yellow pigment, belong to field of fermentation engineering.The present invention can effectively control the approach of Monascus biosynthesis pigment by the species and content of the carbon and nitrogen sources in fermentation medium, the citraurin that Monascus is generated is not then converted to monascorubin；On the other hand, double liquid phase extraction fermentation coupling system can ensure normal growth and the fermentation of Monascus, it is favorably improved dissolved oxygen level, and it is extracellular that monascorubin can be promoted to be secreted into, reduce the negative feedback inhibition effect of red koji fermentation product, so as to greatly improve the fermentation level of monascus yellow pigment, shorten fermentation time.After fermentation ends, product is easily isolated.Using method provided by the invention, the yield and production efficiency of monascus yellow pigment can be greatly improved, reduces production cost.
Description
Technical field
The present invention relates to a kind of biliquid phase fermentation method for being coupled situ extracting fermentation monascus yellow pigment, belong to Fermentation Engineering
Field.
Background technology
Natural yellow pigment is divided into plant extracts and microbial fermentation product.At present caused by the fermentation of energy Large-scale microbial
Natural yellow pigment is mainly carotenoid and monascus yellow pigment.Monascus yellow pigment is divided into two kinds, and one kind is Monascin
（monascine）, another kind is Monascus anka flavine（ankaflavine）.The wavelength one of the maximum absorption band of monascus yellow pigment
As in 400-440nm.In addition in the market also has a kind of also from monascorubin but through the uranidin obtained by chemical modification, i.e., with day
Right monascorubin is raw material, and it is reacted in alkaline solution with sodium dithionite, makes ester hydrolysis in monascorubin, together
When sulfonic group is introduced in pigmentary structures, obtain " water-soluble monascus yellow pigment ", ripple corresponding to the maximum absorption band of this pigment
Length is 476nm.From production method and the structure of pigment, this so-called monascus yellow pigment is that a kind of half-natural red yeast rice is yellow
Pigment.
It is the research and development of current monascorubin industry using the natural monascus yellow pigment of Monascus Production by Microorganism Fermentation
Emphasis.To improve the monascus yellow pigment level of production, the paper of domestic and foreign literature report or the emphasis of patent concern are concentrated mainly on
This two aspects of the mutagenesis screening of high yield monascus strain and the improvement of regular fermentation process condition, but produce effects less.Such as China
Ma Meirong, Thailand Yongsmith Research Team are from having passed through mutagenic and breeding to per unit area yield uranidin since the 1990s
Monascus, applied to liquid state fermentation and solid state fermentation production monascus yellow pigment.The maximum suction of the monascus yellow pigment of bacterial strain production
Wavelength corresponding to receiving peak is 370nm.But the liquid fermentation method uranidin level of production is very low, industrial metaplasia is not yet realized so far
Production.The red yeast rice of bacterium solid state fermentation production in 12 days is yellow（Rice）Color value reaches 2224.63U/gdw, although pigment production level is higher,
But fermentation time is long.Domestic cycle et al. has gone out monascus yellow pigment producing strains by mutagenic and breeding, the red yeast rice of bacterial strain production
Wavelength corresponding to the maximum absorption band of uranidin is 410nm, the zymotic fluid uranidin color value value only up to 110U/ of liquid state fermentation 7 days
mL.More than several due to fermentation pigment concentration it is relatively low, fermentation time is partially long, does not meet the requirement of industrialized production.
The content of the invention
The problem to be solved in the present invention is to provide a kind of Two Liquid Phases fermentation side for being coupled situ extracting fermentation monascus yellow pigment
Method, it is to access Monascus liquid seeds in fermentation medium, fermentation production monascus yellow pigment；The fermentation medium be aqueous phase and
The double liquid phase extraction fermentation system of phase composition is extracted, the aqueous phase is the basic culture containing nutritional ingredient needed for red yeast rice bacteria growing
Base, the extraction phase contain glycerides.
The red yeast rice strain is the red yeast rice strain that can produce monascus yellow pigment.
The minimal medium contains cornstarch 20-80g/L, analysis for soybean powder 0.05-10g/L, ammonium sulfate 3-10g/L,
NaNO32-6g/L, yeast extract 0.05-5g/L, corn starch 0.05-10g/L, zinc sulfate 0.1-0.5g/L, magnesium sulfate 0.5-
1.5g/L, potassium dihydrogen phosphate 0.5-1.5g/L.
Glycerides in the extraction phase are improved the effect of dissolved oxygen as extractant, can will be attached to zymotic fluid
In and the pigment extraction of Monascus ball surface be enriched in extractant phase, increase substantially the production of red yeast rice liquid state fermentation synthetic yellow pigment
The concentration and yield of thing.
The glycerides are triglyceride materials, and dosage volume accounts for the 10-40% of minimal medium volume.
The triglyceride material is tricaprylin, three isooctyl acid glyceride, vegetable oil or its mixture.Except plant
Outside thing oils, remaining triglyceride material addition time is after the inoculation of red yeast rice seed liquor after 24-72h.
The glycerides remove triglyceride material, while also include di-glycerides material, monoglyceride class
Material or its mixture.The di-glycerides material, the addition time of monoglyceride class material are inoculated with 24- for red yeast rice seed liquor
After 72h.When individually adding di-glycerides material or monoglyceride class material, the volume of addition accounts for minimal medium volume
0.1-5%；During mixing addition, cumulative volume accounts for the 0.1-5% of minimal medium volume.
The di-glycerides material is 1,3- diglycerides, 1,2- diglycerides, two glycerol caprylates or its mixture.
The monoglyceride class material is Capmul MCM C8.
The condition of the fermentation production monascus yellow pigment is preferably by the Monascus liquid seeds in exponential phase with volume
It is 80~180r/min in rotating speed in 500mL triangular flasks of the inoculum concentration access equipped with 100mL fermentation mediums than 5-10%, temperature
Under the conditions of 26~32 DEG C of degree, shake flask fermentation culture 4-6d.The Ester of partial glycerol three, the glycerine two of thalli growth can be suppressed
Ester, the addition time of monoglyceride are 24~72h after the inoculation of red yeast rice seed liquor.
Monascus synthesizes citraurin first when fermenting, and the metabolism of red yeast rice citraurin is divided into two branch's approach, an approach
Citraurin is reduced to monascus yellow pigment, and another approach is then water soluble characteristic more preferably red yeast rice with amino acid converting in aqueous phase
Haematochrome.If blocking red yeast rice citraurin to be converted into monascorubin, the citraurin generated may be largely or even complete
Portion is converted into monascus yellow pigment.
The content and Two Liquid Phases that the present invention passes through free amino acid in carbon nitrogen source component reasonable in design, control culture medium
Fermentation system, conversion of the red yeast rice citraurin to monascorubin is blocked, and the monascorubin of synthesis is enriched to rapidly extraction phase
In, so as to improve the production efficiency of monascus yellow pigment, gained monascus yellow pigment color value reaches as high as 250U/mL.Using common
Extractant of the grease as Two Liquid Phases fermentation system, on the one hand because grease plays the role of to increase dissolved oxygen, this is supplied oxygen for guarantee,
The yield for improving pigment is beneficial；On the other hand using vegetable oil as extractant, be advantageous to pigment it is special as vegetable oil
Toner.In addition, glycerolipid material can improve somatic cells membrane permeability, the monascorubin of synthesis is set to be secreted into mycelium in time
Outside, the negative feedback inhibition effect of monascorubin product is reduced.The purity of monascus yellow pigment of the present invention is higher, and fermentation time is short, real
Now efficient, high yield monascus uranidin, increases economic efficiency.
Brief description of the drawings
The monascus yellow pigment full wavelength scanner figure extracted in Fig. 1 zymotic fluids
Embodiment
Following examples bacterial strain uses therefor and fermentation method are according to described in such scheme, using coupling situ extracting fermenting and producing
Monascus yellow pigment product, is further illustrated below embodiments of the invention.
The assay method of uranidin color value：
（1）The processing of zymotic fluid
The measure of zymotic fluid weight and volume and the determination of proportionality coefficient：To the triangular flask that ferments（Jump a queue）In itself and plus cultivate
Base（Including oil phase）Triangular flask afterwards is weighed（W is recorded as respectively0,W2）, show that culture medium weight (is recorded as W1)；Measure culture
Matrix accumulates (V1)；Calculate volume and culture medium weight ratio example coefficient（mL/g）.
After fermentation ends, fermentation flask and zymotic fluid are weighed, the weight before complementing to fermentation with water.By red yeast rice fermented liquid
（Oil-containing）It is transferred completely into fiberizer, wears into homogenate.Take a beaker and one that the merging of metal spoon is weighed, it is suitable with metal bale-out
In 10mL or so zymotic fluid, then by beaker+metal spoon and samples weighing, the actual weight of sample is drawn, and be converted into fermentation
The volume of liquid.Take absolute alcohol about 20mL, be added in beaker, by fermentation broth agitation it is uniform after, be transferred in 50mL colorimetric cylinders,
It is secondary respectively again respectively to take 10mL absolute ethyl alcohols to wash beaker, the remaining zymotic fluid in beaker is transferred completely into colorimetric cylinder, and
Constant volume extracts 1h to 50mL, 55 DEG C of water-baths, and middle interval is shaken 2 times, and water-bath is cooled to room temperature after terminating and taken as needed on 10mL
The ethanol dilutions for stating zymotic fluid are once diluted again according to the method described above.
（2）Yellow valency is determined and calculated
Suitable multiple is diluted again as requested to above-mentioned dilution with absolute ethyl alcohol（By OD values control 0.2-0.8 it
Between）, and full wavelength scanner is carried out in the range of 200-600nm by blank control of absolute ethyl alcohol, determine peak between 400-440nm
The OD values of value.
Yellow valency=OD420nm× total extension rate（U/mL）
Seed culture condition：
Slant medium：Potato 200g/L, glucose 20g/L, magnesium sulfate 0.1g/L, potassium dihydrogen phosphate 0.5g/L, egg
White peptone 5g/L, agar 20g/L.
Liquid seed culture medium：Cornstarch 20g/L, analysis for soybean powder 2.5g/L, ammonium sulfate 3g/L, NaNO36g/L, yeast extract
2g/L, corn starch 2g/L, magnesium sulfate 0.8g/L, potassium dihydrogen phosphate 0.6g/L.
Red yeast rice strain is transferred in slant medium and cultivated, 30 DEG C of cultivation temperature, cultivates 1 week, obtains fresh Monascus, nothing
Bacterium is operated, and lower spore is washed from inclined-plane with a certain amount of sterilized water, prepares spore suspension, is accessed with the inoculum concentration of volume ratio 10%
In 500mL triangular flasks equipped with 100mL liquid seed culture mediums, it is placed in 30 DEG C of constant-temperature tables, rotating speed 120r/min, cultivates
2-3d, obtain the red yeast rice seed liquor in growth logarithmic phase.
Embodiment 1
It is shaking flask strain to select Monascus JJLY-4A.Fermentation medium is made up of minimal medium and extraction phase.Substantially
Culture medium（g/L）：Cornstarch 60, analysis for soybean powder 2, ammonium sulfate 8, NaNO36, yeast extract 0.2, corn starch 0.2, zinc sulfate
0.2, magnesium sulfate 0.8, potassium dihydrogen phosphate 1.2；Extraction phase（With the volume ratio of culture medium）：Soybean oil 10%, corn oil 8%, three are pungent
Acid glyceride 1%, two glycerol caprylates 0.3%, glycerol caprylate 0.2%.In extraction phase in addition to soybean oil and corn oil, remaining
Glycerides add culture medium after fermentation starts 48h.
Monascus liquid seeds in exponential phase are with the inoculum concentration access of volume ratio 10% equipped with 100mL fermentation trainings
It is 180r/min in rotating speed in the 500mL triangular flasks for supporting base, under the conditions of 30 DEG C of temperature, shake flask fermentation culture 5d.Measured after end
The color value of the monascus yellow pigment of minimal medium volume is folded between 220-230U/mL.Pigment detection wavelength is in 400-
Between 440nm nm.
Embodiment 2
It is identical with the experimental procedure of above-described embodiment 1 and fermentation medium, except that：Experimental strain is Monascus
ZH6.Monascus liquid seeds in exponential phase are equipped with 100mL fermented and cultureds with the inoculum concentration access of volume ratio 10%
It is 180r/min in rotating speed in the 500mL triangular flasks of base, under the conditions of 30 DEG C of temperature, shake flask fermentation culture 5d.Folding is measured after end
The color value of the monascus yellow pigment of minimal medium volume is closed between 160-170U/mL.
Embodiment 3
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Cornstarch is 40g/L in fermentation medium.It is 180r/min in rotating speed, under the conditions of 30 DEG C of temperature, shaking flask hair
Ferment culture 5d.The color value for the monascus yellow pigment for being folded to minimal medium volume is measured after end between 150-160U/mL.
Embodiment 4
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Cornstarch is 70g/L in fermentation medium.It is 180r/min in rotating speed, under the conditions of 30 DEG C of temperature, shaking flask hair
Ferment culture 5d.The color value for the monascus yellow pigment for being folded to minimal medium volume is measured after end between 240-250U/mL.
Embodiment 5
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Nitrogen source ammonium sulfate 3g/L in fermentation medium, NaNO33g/L, analysis for soybean powder 6, yeast extract 6g/L.In rotating speed
For 180r/min, under the conditions of 30 DEG C of temperature, shake flask fermentation culture 5d.The red yeast rice for being folded to minimal medium volume is measured after end
The color value of uranidin is between 160-170U/mL.
Embodiment 6
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Nitrogen source ammonium sulfate 8g/L in fermentation medium, NaNO36g/L, analysis for soybean powder 2, yeast extract 2g/L.In rotating speed
For 180r/min, under the conditions of 30 DEG C of temperature, shake flask fermentation culture 5d.The red yeast rice for being folded to minimal medium volume is measured after end
The color value of uranidin is between 200-210U/mL.
Embodiment 7
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Extraction phase（With the volume ratio of culture medium）Match and be：Soybean oil 6%, corn oil 6%, tricaprylin 1%, two
Glycerol caprylate 0.2%, glycerol caprylate 0.1%.It is 180r/min in rotating speed, under the conditions of 30 DEG C of temperature, shake flask fermentation culture 5d.
The color value for the monascus yellow pigment for being folded to minimal medium volume is measured after end between 190-200U/mL.
Embodiment 8
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Extraction phase（With the volume ratio of culture medium）Match and be：Soybean oil 12%, corn oil 12%, tricaprylin 2%,
Two glycerol caprylates 0.5%, glycerol caprylate 0.3%.It is 180r/min in rotating speed, under the conditions of 30 DEG C of temperature, shake flask fermentation culture
5d.The color value for the monascus yellow pigment for being folded to minimal medium volume is measured after end between 230-240U/mL.
Embodiment 9
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Extraction phase（With the volume ratio of culture medium）Match and be：Rapeseed oil 10%, peanut oil 8%, tricaprylin
0.5%th, two glycerol caprylates 0.3%, glycerol caprylate 0.2%.It is 180r/min in rotating speed, under the conditions of 30 DEG C of temperature, shake flask fermentation
Cultivate 5d.The color value for the monascus yellow pigment for being folded to minimal medium volume is measured after end between 220-230U/mL.
Embodiment 10
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Extraction phase（With the volume ratio of culture medium）Match and be：Cottonseed oil 10%, soybean oil 8.5%, two glycerol caprylates
0.3%th, glycerol caprylate 0.2%.It is 180r/min in rotating speed, under the conditions of 30 DEG C of temperature, shake flask fermentation culture 5d.Measured after end
The color value of the monascus yellow pigment of minimal medium volume is folded between 220-230U/mL.
Embodiment 11
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Extraction phase（With the volume ratio of culture medium）Match and be：Tricaprylin 9.5%, three isooctyl acid glyceride 9%,
Two glycerol caprylates 0.3%, glycerol caprylate 0.2%.It is 180r/min in rotating speed, under the conditions of 30 DEG C of temperature, shake flask fermentation culture
5d.The color value for the monascus yellow pigment for being folded to minimal medium volume is measured after end between 120-130U/mL.
Embodiment 12
Using Monascus JJLY-4 as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription, institute
Unlike：Extraction phase（With the volume ratio of culture medium）：Soybean oil 10%, corn oil 8%, tricaprylin 0.5%, 1,3- are sweet
Oily diester 0.3%, glycerol caprylate 0.2%.It is 180r/min in rotating speed, under the conditions of 30 DEG C of temperature, shake flask fermentation culture 5d.Terminate
The color value for the monascus yellow pigment for being folded to minimal medium volume is measured afterwards between 220-230U/mL.
Embodiment 13
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Extraction phase（With the volume ratio of culture medium）：Soybean oil 10%, corn oil 8%, tricaprylin 0.5%, 1,2-
Diglyceride 0.2%, two glycerol caprylates 0.1%, glycerol caprylate 0.2%.It is 180r/min in rotating speed, under the conditions of 30 DEG C of temperature,
Shake flask fermentation culture 5d.The color value that the monascus yellow pigment for being folded to minimal medium volume is measured after end is 220-230U/mL
Between.
Embodiment 14
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Extraction phase（With the volume ratio of culture medium）：Soybean oil 10%, corn oil 8%, tricaprylin 0.5%, 1,3-
Diglyceride 0.1%, 1,2- diglycerides 0.1%, two glycerol caprylates 0.1%, glycerol caprylate 0.2%.It is 180r/ in rotating speed
Min, under the conditions of 30 DEG C of temperature, shake flask fermentation culture 5d.The monascus yellow pigment for being folded to minimal medium volume is measured after end
Color value between 220-230U/mL.
Embodiment 15
Using Monascus JJLY-4A as shake flask fermentation strain.It is essentially identical with the step of above-described embodiment 1 and culture medium prescription,
Except that：Extraction phase（With the volume ratio of culture medium）Match and be：Corn oil 20%.It is 180r/min in rotating speed, 30 DEG C of temperature
Under the conditions of, shake flask fermentation culture 5d.The color value that the monascus yellow pigment for being folded to minimal medium volume is measured after end is 210-
Between 220U/mL.
The common fermentation processes of embodiment 16 fermentation production monascus yellow pigment
Monascus yellow pigment liquid state fermentation process：PDA inclined-planes → spore suspension is made → are seeded to seed liquor → culture
48h, fermentation medium is accessed, after inoculum concentration is 5% → culture 5 days, fermentation liquor pretreatment, colour examining valency.
Slant medium（PDA culture medium）：Potato 200g adds boiling to boil 30min, glucose 20g, 15~20g of agar,
Constant volume 1000mL, natural pH.
Seed culture medium（g/L）：Cornstarch 40, ammonium sulfate 4, KH2PO4, 2.0；K2HPO4, 2.0；MgSO4·7H2O,
0.5；CaCl2, 0.1；FeSO4·7H2O, 0.01；ZnSO4·7H2O, 0.01；MnSO4·H2O, 0.03；NaNO3, 2.0；pH5.0.
Liquid Medium of shaking flask fermentation（g/L）：Cornstarch, 60.0；Ammonium sulfate, 4.0；KH2PO4, 2.0；K2HPO4,
2.0；MgSO4·7H2O, 0.5；CaCl2, 0.1；FeSO4·7H2O, 0.01；ZnSO4·7H2O, 0.01；MnSO4·H2O, 0.03；
NaNO3, 2.0；Initial ph value is 5.0；Fermentation time is 4.0d.
Cultural method：
The preparation of spore suspension：Slant strains one are taken, appropriate amounts of sterilized water and a small amount of bead are added, fully after vibration
Filtered with aseptic filter paper, then counted with blood counting chamber, spore concentration is reached 106Individual/mL.
Monascus seed culture：250mL triangular flask liquid amount 45mL, inoculum concentration：Spore suspension 2mL.30 DEG C reciprocating
Shaking table, stroke 10cm, 100r/min shaken cultivation 48h.
Monascus shaking flask liquid culture：Liquid amount 100mL（500mL triangular flasks）, inoculum concentration 5%.30 DEG C, reciprocal shaker
100r/min, incubation time 5 days.Shake flask fermentation color value value：50.65U/mL.
From above-described embodiment, in the biliquid phase fermentation method of coupling situ extracting fermentation monascus yellow pigment, on an equal basis
Under fermentation condition, the key factor for influenceing monascus yellow pigment yield and yield is that medium component is trained with when extraction phase and substantially
Support the Two Liquid Phases coupling extractive fermentation system that base is formed.In terms of Medium Proportion, higher carbon source concentration, higher inorganic nitrogen
Source concentration can improve zymotic fluid uranidin color value, and organic nitrogen source excessive concentration reduces uranidin yield.Added in fermentation medium
Uranidin color value can be significantly improved during the extractant of more amount.By research obtain preferred process be using Monascus JJLY-4A as
Fermented bacterium, work as minimal medium（g/L）For：Cornstarch 70, analysis for soybean powder 2, ammonium sulfate 6, NaNO34, yeast extract 2, corn steep liquor
Powder 2, magnesium sulfate 0.8, potassium dihydrogen phosphate 1.2；Extraction phase（With the volume ratio of culture medium）：Soybean oil 10%, corn oil 8%, three are pungent
When acid glyceride 1%, two glycerol caprylates 0.3%, glycerol caprylate 0.2%, ferment 5 days, uranidin color value can reach 240-
250U/mL.In addition, confirming through research, method of the invention can be suitably used for all Monascus that can produce monascus yellow pigment（It is such as purple
Color Monascus Monascus purpureus CICC5022, Monascus ruber Monascus rubber CICC40711）, but it is right
In different strains, method of the invention promotes the degree of yield different.The yield of Monascus JJLY-4A fermentation monascus yellow pigments
It is higher than Monascus ZH6's.Domestic existing level is significantly higher than using monascus yellow pigment color value obtained by this new fermentation process
（110U/mL）, and fermentation time reduction is 5 days.This method passes through extractant while Monascus ferments synthetic dyestuff product
By product monascus yellow pigment and thalline and separation of fermentative broth, product negative feedback inhibition is released in time, so as to significantly improve red yeast rice Huang
The yield and yield of pigment, while the separation and Extraction of later stage pigment is also convenient for, the also recyclable recycling of extractant, so as to reduce
Production cost.In summary, method of the present invention is a kind of monascus yellow pigment liquid that is efficient, economic, possessing novelty
Fermentation process, there is important practical value and economic value in monascus yellow pigment production field.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, it can all do various change and modification, therefore the protection model of the present invention
Enclose being defined of being defined by claims.
Claims (9)
1. a kind of biliquid phase fermentation method for being coupled situ extracting fermentation monascus yellow pigment, it is characterised in that be by red yeast rice strain
In son access fermentation medium, fermentation production monascus yellow pigment；The fermentation medium is the Two Liquid Phases of aqueous phase and extraction phase composition
Extractive fermentation system, the aqueous phase are the minimal mediums containing nutritional ingredient needed for red yeast rice bacteria growing, and the extraction phase contains
Glycerides；
The minimal medium contains cornstarch 20-80g/L, analysis for soybean powder 0.05-10g/L, ammonium sulfate 3-10g/L, NaNO3 2-
6g/L, yeast extract 0.05-5g/L, corn starch 0.05-10g/L, zinc sulfate 0.1-0.5g/L, magnesium sulfate 0.5-1.5g/L, phosphorus
Acid dihydride potassium 0.5-1.5g/L；
The glycerides are triglyceride materials, and the volume of addition accounts for the 10-40% of minimal medium volume.
2. according to the method for claim 1, it is characterised in that the triglyceride material is tricaprylin, three
Isooctyl acid glyceride, vegetable oil or its mixture.
3. according to the method for claim 1, it is characterised in that the glycerides also include di-glycerides thing
Matter, the di-glycerides material addition time are that red yeast rice seed liquor is inoculated with after 24-72h, the di-glycerides material
Volume accounts for the 0.1-5% of minimal medium volume.
4. according to the method for claim 1, it is characterised in that the glycerides also include monoglyceride class thing
Matter, the monoglyceride class material addition time are that red yeast rice seed liquor is inoculated with after 24-72h, the monoglyceride class material
Volume accounts for the 0.1-5% of minimal medium volume.
5. according to the method for claim 1, it is characterised in that the glycerides also include di-glycerides and sweet
The single Ester of oil, di-glycerides and monoglyceride class material the addition time be red yeast rice seed liquor be inoculated with 24-72h it
Afterwards, the cumulative volume of diglyceride and monoglyceride accounts for the 0.1-5% of minimal medium volume.
6. the method according to claim 3 or 5, it is characterised in that the di-glycerides material be 1,3-DAG,
1,2- diglycerides, two glycerol caprylates or its mixture.
7. the method according to claim 4 or 5, it is characterised in that the monoglyceride class material is Capmul MCM C8.
8. according to the method for claim 2, it is characterised in that the vegetable oil is soybean oil, corn oil, rapeseed oil, flower
Oil generation, Cottonseed Meal oil, tea-seed oil or other edible oils or its mixture.
9. according to the method for claim 1, it is characterised in that it is described fermentation production monascorubin condition be：It will be in
The Monascus liquid seeds of exponential phase are equipped with 100mL fermentation mediums with volume ratio 5-10% inoculum concentration access
It is 80~180r/min in rotating speed in 500mL triangular flasks, under the conditions of 26~32 DEG C of temperature, shake flask fermentation culture 4-6d.
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| CN108251457A (en) * | 2017-12-23 | 2018-07-06 | 北京工商大学 | A kind of composite extractant and the method for promoting monascorubin production using composite extractant |
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| CN114350526A (en) * | 2022-01-14 | 2022-04-15 | 北京工商大学 | Method for improving monascus pigment tone |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101235353A (en) * | 2007-12-14 | 2008-08-06 | 华南理工大学 | Monascus mutant and method for preparing flavochrome by fermenting the same |
| CN102827895A (en) * | 2012-09-07 | 2012-12-19 | 江南大学 | Double-liquid-phase fermentation method of coupling in-situ extraction antrodia camphorate active product antrondin C |
-
2014
- 2014-03-26 CN CN201410116854.6A patent/CN104946699B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101235353A (en) * | 2007-12-14 | 2008-08-06 | 华南理工大学 | Monascus mutant and method for preparing flavochrome by fermenting the same |
| CN102827895A (en) * | 2012-09-07 | 2012-12-19 | 江南大学 | Double-liquid-phase fermentation method of coupling in-situ extraction antrodia camphorate active product antrondin C |
Non-Patent Citations (3)
| Title |
|---|
| 双液相发酵体系氧传递过程分析;李元广等;《生物工程进展》;19940830;第14卷(第4期);第31页右栏第2段和第32页右栏第1段 * |
| 红曲橙色素的研究;张慧娟等;《中国优秀硕士学位论文全文数据库》;20070115;第3页倒数第2段和第10页2.2.1.3部分 * |
| 黄色素提取工艺的研究进展;呼延晓颖等;《饮料工业》;20110228;第14卷(第2期);9-12 * |
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