CN102827780B - Mortierella alpine strain for producing arachidonic acid - Google Patents
Mortierella alpine strain for producing arachidonic acid Download PDFInfo
- Publication number
- CN102827780B CN102827780B CN 201210360134 CN201210360134A CN102827780B CN 102827780 B CN102827780 B CN 102827780B CN 201210360134 CN201210360134 CN 201210360134 CN 201210360134 A CN201210360134 A CN 201210360134A CN 102827780 B CN102827780 B CN 102827780B
- Authority
- CN
- China
- Prior art keywords
- arachidonic acid
- mortierella
- strain
- mortierella alpine
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a mortierella alpine strain for producing arachidonic acid, belonging to the technical field of bioengineering. The strain is classified and named as mortierella alpine JN168, and is preserved in China center for type culture collection (CCTCC) with the CCTCC No. M2012073. The mortierella alpine strain for producing the arachidonic acid is obtained by separating and screening soil in forests of Hyesan in Wuxi of Jiangsu Province, is stable in characters and can produce the arachidonic acid with high yield by a fermentation method.
Description
Technical field
The Mortierella alpine trichoderma strain of one strain yield peanut tetraenoic acid belongs to technical field of bioengineering.The present invention relates to a kind of Mortierella alpine trichoderma strain of high-yield peanut tetraenoic acid, this strain growth speed is fast, output is high, for arachidonic suitability for industrialized production significance is arranged.
Background technology
The arachidonic acid system is called all-cis formula △-5,8,11, and the 14-eicosatetraenoic acid belongs to the polyunsaturated fatty acid of n-6 series, is the metabolism hinge of this series polyunsaturated fatty acid., distribute the widest polyunsaturated fatty acid maximum as content in the human body, arachidonic acid has significant physiological and pharmacological effect.Studies show that: arachidonic acid has good biological activity at aspects such as promoting brain growth, reducing blood-fat, anti-inflammatory and the oxidation of lipotropism matter.Based on arachidonic these vital role, it has been widely used in aspects such as food, medicine.The someone points out again in recent years: arachidonic meta-bolites also is being with a wide range of applications aspect prevention, the treatment tumour.Arachidonic acid is present in animal, the microorganism widely.Present arachidonic main source comprises fish oil, animal tissues etc., but its content is low, the high serious arachidonic suitability for industrialized production that hindered of extraction cost.Utilize the fermentative Production arachidonic acid to have content height, short, the easy advantage of extraction of cycle, be subjected to the favor of Chinese scholars.Lizuka(" Production of arachidonic acid by a hydrocarbon-utilizing strain of in 1979 according to the literature
Penicillium cyaneum", Applied Microbiology and Biotechnology, 1979,2 (7), 173-180) utilize at first
Penicillium cyaneuCarried out microbial fermentation production Study on Arachidonic Acid.By discover Conidiobolus in micro-algae, part bacterium, yeast and the phycomycetes (
Conidiobolus), genus mortierella (
Mortierella), Mucor (
Mucor), Rhizopus (
Rhizopus), Thamnidium (
Diasporangium) waiting can both the fermentative production arachidonic acid, especially with Mortierella alpina research at most (" microbial fermentation production Study on Arachidonic Acid progress ", Chinese oil, 2004,9 (29), 7-40).Be rich in polyunsaturated fatty acids such as arachidonic acid in the filamentous fungus Mortierella alpina mycelium, its arachidonic acid content height, purification conveniently are acknowledged as and produce arachidonic strain excellent.
The arachidonic acid structural formula
Patent CN101113410A has introduced a kind of Mortierella alpine trichoderma strain and application thereof of high-yield peanut tetraenoic acid.Patent JP2008113671, patent US5658767A, Shen Yiling (" Mortierella alpina fermentative production arachidonic acid Study on Conditions ", food science and technology, 2009,2 (34), 35-38) and Totani(" The filamentous fungus
Mortierellaalpina, high in arachidonic acid ", Lipids, 1987,22,1060 – 1062) etc. also reported and utilize Mortierella alpina fermentation to produce Study on Arachidonic Acid.
But in existing report, owing to be subjected to the influence of factors such as bacterial classification quality, fermentation condition, extracting mode, arachidonic output is very different, and fermentation period also is not quite similar.Chen(" Optimization of Arachidonic Acid Productionby in 1997
Mortierella alpinaWuji-H4 Isolate ", Journal of the American Oil Chemists'Society, 1997,5 (74), 569-578) grade obtains a strain arachidonic acid through the low temperature screening and produces bacterium
Alpina Wuji-H4, shake flask fermentation after 5 days its arachidonic acid yield reach 3.9g/L.Patent CN101113410A provides a plant height mountain mortierella
ME-AA01, after 6.5 days, the arachidonic acid yield is brought up to 10g/L by shake flask fermentation for it.(" A novel two-step fermentation process for improved arachidonic acid production by such as Jin
Mortierella alpina", Biotechnol Lett, 2008,30 (6), 1087 – 1091) utilize bacterial strain
M. alpina ME-1Adopt ethanol to coerce and two-step fermentation production arachidonic acid, after 7 days, the arachidonic acid yield can reach 19.8g/L through the 5L ferment tank.
But the present invention screens from the soil of favour mountain, the Wuxi City, Jiangsu Province woods and to have obtained that proterties is stable, fermentation period is short, 30 ℃ down the Mortierella alpine trichoderma strain of high-yield peanut tetraenoic acids (
Mortierella alpina) JN168.Has better industrial application prospect.
Summary of the invention
The Mortierella alpine trichoderma strain that the purpose of this invention is to provide a strain yield peanut tetraenoic acid, it is stable to have obtained proterties, and the Mortierella alpine trichoderma strain of yield peanut tetraenoic acid can be produced arachidonic acid by solid-state or liquid state fermentation.
Technical scheme of the present invention: the Mortierella alpine trichoderma strain of a strain yield peanut tetraenoic acid, the called after Mortierella alpina (
Mortierella alpina) JN168, being preserved in Chinese typical culture collection center, preserving number is CCTCC NO:M2012073.Its 18S rDNA sequence is SEQ ID NO:1.
This bacterial strain main metabolites is arachidonic acid.
The available carbon source of this bacterial strain is glucose, fructose, maltose, sucrose, lactose, D-semi-lactosi, glycerine, Zulkovsky starch or yam starch.
The available nitrogenous source of this bacterial strain is inorganic ammonium salt, inorganic nitrate, yeast powder, yeast extract paste, peptone, groundnut meal or soybean cake powder.
In favour mountain, the Wuxi City, Jiangsu Province woods, choosing fallen leaves and covering closeer soil, remove veneer of soil, vertically shovel with the sampling shovel and get 10 centimetres of depths soil sampling box of packing into.Take by weighing 1g left and right sides soil sample and in the 50mL centrifuge tube, add the 9mL deionized water dissolving, get the 1mL lysate and join in the centrifuge tube that the 9mL deionized water is housed, and the like make 10
-1, 10
-2, 10
-3, 10
-4The bacteria suspension of various gradients gets 10
-2, 10
-3, 10
-4Three gradients are carried out the flat board coating, are placed on to be inverted in 30 ℃ the biochemical incubator to cultivate.Separate and with substratum be: potato 200g, glucose 10g, agar 20g, water 1000mL, penicillin 20mg, pH nature.30 ℃ of constant temperature.After treating that single bacterium colony grows, picking list bacterium colony sudan black B stain, fat particle is dyed bluish voilet, selects the many and darker bacterial strain of dyeing of fat particle and shakes the multiple sieve of bottle.The mycelium suction filtration of results shake-flask culture, lyophilize is to constant weight, grinds mycelium, extracts grease with cable-styled extraction method, to carry grease carry out the MS-GS coupling and analyze the lipid acid composition.Adopt above method repeatedly, obtain the arachidonic acid high yielding strain strain through repeatedly screening
Mortierella alpinaJN168.
The bacterial classification that the present invention relates to has following feature
1, Mortierella alpina
Mortierella alpinaThe JN168 culture condition
This culture of strains normal condition has been that air culture is supported.The saccharine material that is used for the cultivation bacterial classification can be glucose, fructose, maltose, sucrose, lactose, D-semi-lactosi, glycerine, Zulkovsky starch or yam starch.The nitrogenous source of cultivating usefulness can be inorganic ammonium salt, inorganic nitrate, yeast powder, yeast extract paste, peptone, groundnut meal or soybean cake powder.The growth temperature range of this bacterial strain is 5 ℃-35 ℃, and the best accumulation of arachidonic acid temperature is 30 ℃, and bacterial strain can be grown in pH is the 2-9 scope and metabolism yield peanut tetraenoic acid, and the best accumulation of arachidonic acid pH is 6.5.In the culture of strains process, add inorganic salt such as sodium-acetate, yellow soda ash, sodium bicarbonate, sal epsom, potassium primary phosphate arachidonic output is had promoter action.
2, Mortierella alpina
Mortierella alpinaThe form of JN168 is described
The dull and stereotyped form of cultivating:
Mortierella alpinaJN168 cultivated after 2 days, occurred the thinner white colony of quality on the flat board, and bacterium colony is rounded.Bacterium colony begins to expand layer by layer after 5 days, forms similar petal-like form.The positive white of bacterium colony, the back side is light yellow.(triphenyltetrazolium chloride, on flat board TTC), bacterium colony presents redness containing Triphenyltetrazolium chloride.Behind sudan black B stain, bluish voilet fat particle in the visible mycelium of microscopically.
3, Mortierella alpina
Mortierella alpinaThe 18S rDNA molecular biological characteristics of JN168 is seen sequence table.
4, can adopt conventional liquid state fermentation method, utilize above-mentioned bacterial classification to produce arachidonic acid.Substratum can be with containing ordinary culture mediums such as different carbon sources, nitrogenous source, mineral ion.
Also can add an amount of nutrition to the special needs of employed microorganism growth in the substratum, generalized case, these nutrition are present in the above-mentioned natural nutrition source.
This bacterial strain begins the yield peanut tetraenoic acid after 24 hours in the liquid state fermentation process, the arachidonic acid yield reaches maximum value after 80 hours.
Beneficial effect of the present invention:
Mortierella alpinaThe main metabolites of JN168 is arachidonic acid, and arachidonic acid content reaches more than 50% less side products in the mycelia.The best accumulation of arachidonic acid temperature is 30 ℃, and comparing with other bacterial classification has higher leavening temperature, so fermentation time shortens to 80 hours, and fermenting process is more easy to control.
Also can add an amount of nutrition to the special needs of employed microorganism growth in the substratum, generalized case, these nutrition are present in the above-mentioned natural nutrition source.
The optimum carbon source of this bacterial strain liquid state fermentation yield peanut tetraenoic acid is glucose, and optimum nitrogen source is yeast powder.Can reach 13.0g/L with this substratum arachidonic output under 30 ℃.
In this bacterial strain liquid state fermentation process, begin to produce arachidonic acid after 24 hours, the arachidonic acid yield reaches maximum after 80 hours.
The biological material specimens preservation: Mortierella alpina (
Mortierella alpina) JN168, be preserved in Chinese typical culture collection center, be called for short CCTCC, address: Chinese Wuhan Wuhan University, deposit number is CCTCC NO:M2012073, preservation date is on 03 10th, 2012.
Embodiment
Embodiment 1
The Mortierella alpina fatty acid compositional analysis
Fermentation strain is Mortierella alpina
Mortierella alpinaJN168 adopts liquid state fermentation, adopts standard P DA substratum.30 ℃ of leavening temperatures, secondary fermentation in 7 days finishes.Suction filtration is collected mycelium, and lyophilize grinds mycelium with mortar to constant weight.Get the mycelium of drying about 0.1g, join in the test tube of lid.To the potassium hydroxide that wherein adds 0.4mol/L-methanol solution 5mL, in 50 ℃ of water-baths, keep 1h, again to wherein adding 5mL 14% boron trifluoride-methanol solution, in 50 ℃ of water-baths, keep 1h, add the extracting of 5mL normal hexane, add the washing of 2mL saturated nacl aqueous solution at last, pipette upper solution and adopt the GC-MS coupling to detect.Analytical results is as shown in table 1.The result shows in the grease that this bacterium produces based on arachidonic acid.
Chromatographic condition: the Gas Chromatography-mass Spectrometer (GCMS) Finnigan Trace MS(U.S.).Adopt DB-MAX capillary column (30m * 0.25mm * 0.25 μ m).180 ℃ of initial column temperatures keep 1min, are elevated to 230 ℃ with 5 ℃/min, keep 8min.260 ℃ of injector temperatures, carrier gas are helium, and flow velocity is 0.8mL/min, and splitting ratio is 10 ︰ 1.
The mass spectrometric detection condition: EI+(70Ev), transmitter current 200 μ A, 350 ℃ of ion source temperatures, 260 ℃ of interface temperature.
Table 1 lipid acid is formed
| Title | C16:0 | C16:1 | C18:1 | C18:2 | C18:3 | C20:3 | C20:4 | Other |
| Account for total fatty acids ratio (%) | 5.2 | 5.8 | 9.3 | 8.1 | 10.2 | 2.3 | 58.4 | 0.7 |
Embodiment 2
Determining of optimum carbon source
With the Mortierella alpina that is kept on the inclined-plane
Mortierella alpinaThe JN168 inoculation is in solid PDA substratum, and 30 ℃ of activation are used the aseptic water washing inclined-plane until the spore maturation, make 10
6The spore suspension of individual/mL is inoculated in the liquid seed culture medium with 2% inoculum size, cultivates 1 day for 30 ℃.Be inoculated in the fermention medium with 10% inoculum size, cultivated 80 hours for 30 ℃.Use single factor to investigate the optimum carbon source of arachidonic acid accumulation, the glucose that uses fructose, maltose, sucrose, lactose, D-semi-lactosi, glycerine, Zulkovsky starch or yam starch to substitute in the basic fermention medium respectively carries out liquid state fermentation, measures dry weight and arachidonic acid yield.Liquid seed culture medium and basic fermention medium composition are (w/v, together following): glucose 80, ammonium sulfate 5, sal epsom (MgSO
47H
2O) 0.5, potassium primary phosphate 1.5,6.0,115 ℃ of sterilizations of pH 20min.The optimum carbon source of strain fermentation yield peanut tetraenoic acid is glucose as can be seen from Table 2.
The arachidonic acid condition of production under the different carbon sources of table 2
Embodiment 3
Determining of optimum nitrogen source
With the Mortierella alpina that is kept on the inclined-plane
Mortierella alpinaThe JN168 inoculation is in solid PDA substratum, and 30 ℃ of activation are used the aseptic water washing inclined-plane until the spore maturation, make 10
6The spore suspension of individual/mL is inoculated in the liquid seed culture medium with 2% inoculum size, cultivates 1 day for 30 ℃.Be inoculated in the fermention medium with 10% inoculum size, cultivated 80 hours for 30 ℃.Use single factor to investigate the optimum nitrogen source of arachidonic acid accumulation, the ammonium sulfate that uses ammonium nitrate, yeast powder, yeast extract paste, peptone, groundnut meal or soybean cake powder to substitute in the basic fermention medium respectively carries out liquid state fermentation, measures dry weight and arachidonic acid yield.Basic fermention medium is formed (w/v): glucose 80, ammonium sulfate 5, sal epsom (MgSO
47H
2O) 0.5, potassium primary phosphate 1.5,6.0,115 ℃ of sterilizations of pH 20min.The optimum nitrogen source of strain fermentation yield peanut tetraenoic acid is yeast powder as can be seen from Table 3.
The arachidonic acid condition of production under table 3 different nitrogen sources
| The nitrogenous source kind | Ammonium sulfate | Ammonium nitrate | Yeast powder | Yeast extract paste | Peptone | The peanut muffin | Soybean cake powder |
| Dry cell weight g/L | 28.7 | 26.1 | 36.6 | 35.7 | 35.9 | 34.1 | 33.0 |
| Arachidonic acid yield g/L | 8.84 | 6.90 | 10.63 | 9.54 | 9.62 | 10.03 | 8.42 |
Embodiment 4
Determining of optimum temps
With the Mortierella alpina that is kept on the inclined-plane
Mortierella alpinaThe JN168 inoculation is in solid PDA substratum, and 30 ℃ of activation are used the aseptic water washing inclined-plane until the spore maturation, make 10
6The spore suspension of individual/mL is inoculated in the liquid seed culture medium with 2% inoculum size, cultivates 1 day for 30 ℃.Be inoculated in the fermention medium with 10% inoculum size, cultivated 80 hours.Use single factor to investigate the optimum temps of arachidonic acid accumulation, respectively leavening temperature is adjusted to 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃, measure dry weight and arachidonic acid yield.Fermention medium is formed (w/v): glucose 80, ammonium sulfate 5, sal epsom (MgSO
47H
2O) 0.5, potassium primary phosphate 1.5,6.0,115 ℃ of sterilizations of pH 20min.The optimum temps of strain fermentation yield peanut tetraenoic acid is 30 ℃ as can be seen from Table 4.
The arachidonic acid condition of production under table 4 differing temps
| Temperature | 15 | 20 | 25 | 30 | 35 |
| Dry cell weight g/L | 28.0 | 30.5 | 33.7 | 36.0 | 32.9 |
| Arachidonic acid yield g/L | 6.30 | 7.63 | 9.77 | 10.47 | 9.53 |
Embodiment 5
Best pH determines
With the Mortierella alpina that is kept on the inclined-plane
Mortierella alpinaThe JN168 inoculation is in solid PDA substratum, and 30 ℃ of activation are used the aseptic water washing inclined-plane until the spore maturation, make 10
6The spore suspension of individual/mL is inoculated in the liquid seed culture medium with 2% inoculum size, cultivates 1 day.Be inoculated in the fermention medium with 10% inoculum size, cultivated 80 hours for 30 ℃.Use single factor to investigate the best pH of arachidonic acid accumulation, the pH that will ferment respectively adjusts to 4.5,5.0,5.5,6.0,6.5,7.0,7.5 and measures dry weight and arachidonic acid yield.Fermention medium is formed (w/v): glucose 80, ammonium sulfate 5, sal epsom (MgSO
47H
2O) 0.5,1.5,115 ℃ of sterilizations of potassium primary phosphate 20min.The best pH of strain fermentation yield peanut tetraenoic acid is 6.5-7.5 as can be seen from Table 5.
The arachidonic acid condition of production under the different pH values of table 5
| pH | 4.5 | 5.0 | 5.5 | 6.0 | 6.5 | 7.0 | 7.5 |
| Dry cell weight g/L | 27.1 | 29.6 | 32.3 | 36.5 | 41.4 | 40.7 | 39.9 |
| Arachidonic acid yield g/L | 6.91 | 8.24 | 9.02 | 10.27 | 13.0 | 11.8 | 11.5 |
Embodiment 6
Fermentation strain be (
Mortierella alpina) JN168, adopting solid state fermentation, substratum is formed (weight %): 30 purpose Semen Maydis powder 68.7, wheat bran 8, rice bran 20, glucose 1, KH
2PO
40.25, MgSO
47H
2O 0.05; It is wetting to add the water spice, in the wide-necked bottle of packing into, and the 0.1MPa 45-60min that sterilizes, the cooling back is inserted liquid seeds and is mixed all.30 ℃ of leavening temperatures, after 7,6 days fermentation ends of pH, arachidonic content is 6.4% in the fermention medium.
＜110〉Southern Yangtze University
＜120〉the Mortierella alpine trichoderma strain of a strain yield peanut tetraenoic acid
<160> 1
<210> 1
<211> 609
<212> DNA
＜213〉Mortierella alpina (
Mortierella alpina) JN168
<400> 1
tccgtaggtg aacctgcgga aggatcatta ccaagagaaa tctttcaaca ctgaaagatc 60
tgctggcttt gaccgtatgt aattttggga aaaacaatat ctttaaaatg gttcgcaagg 120
gccggtccca ttttcctttg atcatcctta tctgaacaat taaacaaatg attttaataa 180
tctgtttaaa catttgctcc acaactttca acaacggatc tcttggttct cgcatcgatg 240
aagaacgcag cgaaatgcga tacgtaatgt gaattgcaga attcagtgaa tcatcgaatc 300
tttgaacgca cattgcactc cttggtattc cgaggagtat gcctgtttca gtatcatgag 360
cactctcaca cctaaccttt gggtttatgg cgtggaattg gaatgcgccg actgtcatgg 420
ttggcccttc taaaatgtag ttcttggctg tcacctaata cagcagtttg gcctaatagt 480
tttggcattc attgtcaaat ctttggctaa tgaaattttt aggagtcagt cttgataata 540
cagaaaactc attcaaattt tgatctgaaa tcaggtaggg ctacccgctg aacttaagca 600
tatcaataa 609
Claims (5)
1. the Mortierella alpine trichoderma strain of a strain yield peanut tetraenoic acid, its classification called after Mortierella alpina (
Mortierella alpina) JN168, being preserved in Chinese typical culture collection center, preserving number is CCTCC NO:M2012073.
2. Mortierella alpine trichoderma strain according to claim 1, its 18S rDNA sequence is SEQ ID NO:1.
3. Mortierella alpine trichoderma strain according to claim 1 is characterized in that energy metabolism produces arachidonic acid.
4. Mortierella alpine trichoderma strain according to claim 1 is characterized in that available carbon source is glucose, fructose, maltose, sucrose, lactose, D-semi-lactosi, glycerine, Zulkovsky starch or yam starch.
5. Mortierella alpine trichoderma strain according to claim 1 is characterized in that available nitrogenous source is inorganic ammonium salt, inorganic nitrate, yeast powder, yeast extract paste, peptone, groundnut meal or soybean cake powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210360134 CN102827780B (en) | 2012-09-25 | 2012-09-25 | Mortierella alpine strain for producing arachidonic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210360134 CN102827780B (en) | 2012-09-25 | 2012-09-25 | Mortierella alpine strain for producing arachidonic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102827780A CN102827780A (en) | 2012-12-19 |
| CN102827780B true CN102827780B (en) | 2013-07-17 |
Family
ID=47331094
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210360134 Expired - Fee Related CN102827780B (en) | 2012-09-25 | 2012-09-25 | Mortierella alpine strain for producing arachidonic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102827780B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861339B (en) * | 2016-06-16 | 2019-10-18 | 江南大学 | Recombination Mortierella alpina, its construction method and the application of one plant of overexpression GTP cyclohydrolase gene |
| CN106636236B (en) * | 2017-01-13 | 2020-10-23 | 江南大学 | Fermentation medium for promoting mortierella alpina to produce EPA and application thereof |
| CN108823109A (en) * | 2018-07-24 | 2018-11-16 | 江苏远大仙乐药业有限公司 | A kind of Mortierella alpine trichoderma strain of high-yield peanut tetraene acid lipid and its application |
| CN112625912B (en) * | 2020-09-09 | 2023-06-06 | 中国科学院微生物研究所 | Mortierella alpina strain XY05201 and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101113410A (en) * | 2007-07-09 | 2008-01-30 | 南京工业大学 | Mortierella alpina and uses thereof |
-
2012
- 2012-09-25 CN CN 201210360134 patent/CN102827780B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101113410A (en) * | 2007-07-09 | 2008-01-30 | 南京工业大学 | Mortierella alpina and uses thereof |
Non-Patent Citations (4)
| Title |
|---|
| 丛蕾蕾 等.高山被孢霉产花生四烯酸及其遗传改造的研究进展.《生物工程学报》.2010,第26卷(第9期), |
| 沈以凌 等.高山被孢霉发酵生产花生四烯酸条件的研究.《食品科技》.2009,第34卷(第2期), |
| 高山被孢霉产花生四烯酸及其遗传改造的研究进展;丛蕾蕾 等;《生物工程学报》;20100925;第26卷(第9期);1232-1238 * |
| 高山被孢霉发酵生产花生四烯酸条件的研究;沈以凌 等;《食品科技》;20090228;第34卷(第2期);35-39 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102827780A (en) | 2012-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102864111B (en) | Schizochytrium limacinum strain for producing docosahexaenoic acid | |
| CN103025862B (en) | Novel thraustochytrid-based microalgae, and method for preparing bio-oil by using same | |
| CN104087512B (en) | Produce Mortierella alpina and the application thereof of polyunsaturated fatty acid | |
| CN1986822A (en) | Crypthecodinium connii fermenting process for producing docosahexaenoic acid grease | |
| CN108251339B (en) | One plant of 3-hydroxy-2-butanone superior strain and its application in fermenting and producing 3-hydroxy-2-butanone | |
| CN102827780B (en) | Mortierella alpine strain for producing arachidonic acid | |
| Zhu et al. | pH-control modes in a 5-L stirred-tank bioreactor for cell biomass and exopolysaccharide production by Tremella fuciformis spore | |
| CN103602591B (en) | A kind of schizochytrium limacinum and the method for the production of docosahexaenoic acid grease | |
| CN105420122A (en) | Schizochytrium limacinum suitable for high-density culture and method for producing grease rich in DHA | |
| CN103451247B (en) | A kind of arachidonic preparation method | |
| CN101709297A (en) | Mutagenic screening method of strain mortierella alpina for generating arachidonic acid | |
| CN102559802A (en) | Method for preparing high-yield Tremella polysaccharide by adopting culture medium for fermenting | |
| CN103981106B (en) | DHA superior strain and application thereof | |
| CN104328053A (en) | Scenedesmus capable of highly yielding oil as well as culture method and application thereof | |
| CN101861794A (en) | Method for producing liquid strain of cordyceps militaris | |
| CN101948759B (en) | Mortierella isabellina and application thereof | |
| CN101805760A (en) | Method for producing microbial oil comprising arachidonic acid | |
| CN103805517A (en) | New separated bacterial strain of mortierella alpine and application thereof | |
| US9441252B2 (en) | Process for producing lipids suitable for biofuels | |
| JP5512613B2 (en) | Method for producing oil using yeast of the genus Cystofilobasidium | |
| CN107988104A (en) | The method of the cryptococcus and crude glycerine culture Lipid-producing of one plant of production Unicell Oils and Fats | |
| CN103320331B (en) | Pythium irregulare strain for producing eicosapentaenoic acid | |
| CN104388486A (en) | Preparation method of ARA (arachidonic acid) | |
| CN105820980B (en) | A kind of bacillus of yielding lipase and its application | |
| CN103627646B (en) | Wine yeast with low yield of higher alcohol |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130717 Termination date: 20140925 |
|
| EXPY | Termination of patent right or utility model |