CN101386870B - Method for preparing rhodiola rosea liquid conversion leavening by microbial hybrid bacterial strain - Google Patents
Method for preparing rhodiola rosea liquid conversion leavening by microbial hybrid bacterial strain Download PDFInfo
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- CN101386870B CN101386870B CN2008100463690A CN200810046369A CN101386870B CN 101386870 B CN101386870 B CN 101386870B CN 2008100463690 A CN2008100463690 A CN 2008100463690A CN 200810046369 A CN200810046369 A CN 200810046369A CN 101386870 B CN101386870 B CN 101386870B
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Abstract
The invention provides a method for improving active compositions in a raw medicinal material of rhodiola by transforming and fermenting a mixed microorganism strain in a liquid water suspension of the raw medicinal material of the rhodiola. The method comprises the follow steps: the liquid water suspension of the rhodiola produced by rhodiola rootstalk powder is sterilized and then grafted with the mixed microorganism strain for liquid fermentation and cultivation. The transformation and fermentation by the method can ensure that the active compositions of the rhodiola are greatly improved than those in the raw medicinal material, and ensure that the content of the active compositions is highest and can be improved by more than 100 percent than those in the raw medicinal material, so the method can effectively utilize wild resources, can also reduce the discharge of pollutants such as drug residue and so on, realize the protective development of Chinese Tibetan medicine, and has good application prospect.
Description
Technical field
The invention belongs to from Root of Kirilow Rhodiola and hide the technical field of extracting the effective active composition the Chinese medicinal materials, be specifically related to a kind ofly prepare the method for rhodiola rosea liquid conversion leavening with microbial hybrid bacterial strain, this method improves effective active composition contained in the fermented product greatly.
Background technology
Root of Kirilow Rhodiola is meant the Crassulaceae Rhodida plant, have 90 in the world surplus kind, be distributed in the severe cold areas in the Northern Hemisphere more, great majority all are grown under the high mountain flowstones or bushes about height above sea level 3500-5000 rice.China has now found 73 kinds, mainly is distributed in the high mountain severe cold areas in China Tibet, and also there is the Root of Kirilow Rhodiola resource distribution in some areas in northeast, northwest, North China in addition.
The main medicinal part of Root of Kirilow Rhodiola is a rhizome, wherein contained effective constituent rhodioloside and the tyrosol (glucoside unit) of mainly containing, have effects such as antifatigue, anti-hypoxia, antimutagenic and inhibition tumour, and (Song Yueying etc. have no side effect, Rhodida plant chemical ingredients and Pharmacological action study progress herbal medicine .2004,35 (2) .-235-236; Li Gang waits the pharmaceutical research of Tibetan medicine Root of Kirilow Rhodiola to make progress Chinese national medicine magazine .2004,10 (3) .-38-40; The Advance on Pharmacological Activities Chinese materia medica journal .2003 of the pious Root of Kirilow Rhodiola of the flat Wang Sheng of the bright Yan Ji of king family, 31 (4) .-57-59), therefore, in the exploitation of medicine and protective foods, obtained using widely.
Owing to, make Tibetan area, this plateau resources of natural crude drugs face exhausted crisis to a large amount of development and use of Root of Kirilow Rhodiola.And the Root of Kirilow Rhodiola activeconstituents preparation technology who adopts at present directly soaks the Root of Kirilow Rhodiola raw material with alcohols (ethanol etc.) to extract.This method can only be extracted wherein the effective active composition that exists, and effective active component content wherein is very low, is about 0.6～0.7% as the content of rhodioside.In the face of a large amount of social demands, how on the basis of current extraction method, to develop new preparation method, to improve the utilization ratio of Root of Kirilow Rhodiola crude drug, become current this area urgent problem.The new process of having reported has chemical synthesis, plant cell tissue's culture method and with the microbe transformation method of single bacterial strain.These methods all are before the alcohols extraction process, and desire obtains by different sources or increases effective constituent, and expectation reduces the consumption of wild resource.Wherein microbe transformation method is the method for tool application prospect from the effective constituent that improves Root of Kirilow Rhodiola itself, is exactly the method that employing single microorganism bacterial strain carries out transformation fermentation as the disclosed technology of patent application " a kind of method of preparing rhodiola root extract transformed by microbe " (application number 200610146107.2).Though this method can improve the effective constituent of Root of Kirilow Rhodiola itself, but can only improve effective active component content about 30% because of it is the highest, so improve limited, desirable not enough, especially as having increased the cost that microorganism strains transformation fermentation operation is brought, compare with the value of the effective active component content that is improved, can only be near the breakeven point, it is little that its production application is worth.
Summary of the invention
The objective of the invention is to carry out transformation fermentation and improve the defective that the rhodiola active ingredient method exists at existing employing single microorganism bacterial strain, a kind of method for preparing rhodiola rosea liquid conversion leavening with microbial hybrid bacterial strain is provided, this method can significantly improve effective active composition in the Root of Kirilow Rhodiola crude drug, under the condition of utilizing the isodose crude drug, can obtain the more product that extracts, wild Tibetan Chinese medicinal materials is fully used.
Provided by the inventionly prepare the method for rhodiola rosea liquid conversion leavening with microbial hybrid bacterial strain, the processing step of this method and condition are:
1) the Root of Kirilow Rhodiola rhizome is crushed to 60～120 orders, preferred 80～120 orders, more preferably 80 orders;
2) in per 100 parts Root of Kirilow Rhodiola powder, add 800～1000 parts of water, preferred 800～900 parts, 0.5～2 parts of yeast extract pastes, preferred 1～2 part, 0.5～2 part of potassium primary phosphate, preferred 1～2 part, with 0.2～0.5 part of sal epsom, preferred 0.2 part, mix, make it to become the rhodiola rosea liquid aqueous suspensions, and sterilization;
3) in the liquid seed culture medium that contains the Root of Kirilow Rhodiola powder, prepare the aspergillus niger (Aspergillus niger) in the Aspergillus and the mycelium culture thing of aspergillus oryzae (Aspergillus oryzae) respectively;
The mycelium culture thing of two kinds of moulds that 4) will prepare will mix the mycelia culture and be inoculated in the rhodiola rosea liquid aqueous suspensions after mixing by weight 2～8: 8～2, and inoculum size is counted 5～20 parts of per 100 parts of water inoculations by weight in wet base;
5) postvaccinal prepared product was fermented 48～60 hours in 28～32 ℃ of following heat insulating culture of temperature, 30 ℃ of preferred temperature, incubation time got final product in 50 hours,
More than used material umber be weight part.
More than the weight in wet base of Jie Zhong mycelium culture thing is meant the centrifugal weight of removing behind the moisture of the mycelium culture thing that cultivates.
In this method the rhodiola rosea liquid aqueous suspensions is sterilized and can be carried out under normal condition, as sterilizing 30 minutes for 121 ℃ in temperature.
Used mycelium culture thing is to cultivate like this in this method: be that 80 purpose Root of Kirilow Rhodiola powder and water mix by 1: 10 weight part with particle diameter earlier, do not add 0.2% yeast extract paste again by the score of the weight part of water, 0.1% potassium primary phosphate and 0.05% sal epsom, be made into liquid seed culture medium, in liquid seed culture medium, insert aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) spore in the Aspergillus (Aspergillus) then respectively, cultivated 2 days at 30 ℃ of following shaking tables of temperature, centrifugal back abandoning supernatant is collected the mycelia precipitation.
Two kinds of wherein used moulds both can have been adopted aspergillus niger (Aspergillus niger) and the aspergillus oryzae (Aspergillus oryzae) in the existing Aspergillus (Aspergillus), also can adopt the inventor to obtain after separation and purification and the screening from the wild red red-spotted stonecrop medicinal material, preferred back is a kind of.The inventor from wild red red-spotted stonecrop medicinal material after separation and purification and the screening aspergillus niger (Aspergillus niger) that obtains and the concrete grammar of aspergillus oryzae (Aspergillus oryzae) be: natural wild red red-spotted stonecrop medicinal material is smashed to pieces, get 1g, add the 5ml sterilized water, concussion shakes up, and dilutes 10,100,1000 and 10000 times respectively with sterilized water again.Above each diluent is got 0.1ml respectively, coat in the isolation medium, cultivated 3 days through 30 ℃, picking list bacterium colony is chosen in the Root of Kirilow Rhodiola substratum after the transformation fermentation, detects the bacterial strain that rhodioside content improves with high performance liquid chromatography (HPLC).The separation and Culture based formulas is: glucose 10g, peptone 5g, yeast extract paste 5g, potassium primary phosphate 1g, ammonium sulfate 0.5g, agar 20g and water 1000ml.Isolated two kinds of moulds be accredited as aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) in the Aspergillus (Aspergillus) according to conventional methods.
For the main effectively content of activeconstituents rhodioside in the rhodiola plant, the content of rhodioside answers 〉=0.5% in a regulation of Chinese Pharmacopoeia version in 2005 medicinal material, and the content of rhodioside is about 0.6～0.7% in actual measurement neutral red red-spotted stonecrop crude drug.The preparation technology of existing rhodioside extract adopts alcohols (ethanol etc.) directly to soak to extract, so can only extract the rhodioside that has existed.Because the main effectively activeconstituents rhodioloside of Root of Kirilow Rhodiola and the synthetic secondary metabolism that belongs to of tyrosol (glucoside unit), promptly tyrosol and rhodioloside are to add respectively that on the phenol architecture basics ethanol based and glucosyl group form.The inventor finds to exist certain transformable precursor in the rhodiola plant when research, thereby have abundant metabolism diversity and very strong metabolic capacity according to microorganism, and has a pathways metabolism that in the higher plant body, exists, once the enzyme that had proposed with microorganism is the precursor that comes non-activity in the catalysis Root of Kirilow Rhodiola raw material, make it and synthetic tyrosol of compositions such as glucosyl group and ethanol based and rhodioloside, to improve the technical scheme (number of patent application 200610146107.2) of rhodioloside and tyrosol (glucoside unit) content.The principle of this technical scheme is as shown below:
But because this method employing is single bacterial strain, though can improve the content of effective active composition, its raising amount is comparatively limited.The inventor is in further studying, courageously break the inertial thinking of " it is poorer than single enzyme effect to use mixed enzyme to ferment ", carried out creationary exploration, the above-mentioned method for preparing rhodiola rosea liquid conversion leavening with the aspergillus niger in the Aspergillus (Aspergillus niger) and two kinds of microbial hybrid bacterial strains of aspergillus oryzae (Aspergillus oryzae) is provided.This method can significantly improve effective active composition in the Root of Kirilow Rhodiola crude drug, make the highest comparable crude drug of content of rhodioside improve more than 100%, thereby under the condition of utilizing the isodose crude drug, can obtain more to extract product, wild Tibetan Chinese medicinal materials is fully used.
Because the highest comparable crude drug of the content of rhodioside improves more than 100% in the transformation fermentation thing of gained after the inventive method fermentative processing, thereby when obtaining the isodose product, can reduce crude drug consumption.And can find out in the analytical calculation by following economic benefit that also the synergy of single strain conversion method is near the breakeven point; And present method synergy is 8 times of the single strain conversion method, has very strong application prospect:
By obtaining the equal cubage of rhodioside, directly the former technology with alcohol immersion need consume 1 ton of crude drug, the single strain conversion method needs crude drug 770kg (calculating by improving 30%), and the inventive method only needs crude drug 500kg (calculating by improving 100%).Identical because of the single strain conversion method again with the production cost that the inventive method increases, about 4000 yuan of/ton crude drugs.Need 20,000 yuan by buying crude drug per ton,
The single strain conversion method
Reduce by 20000 yuan/ton * 230kg=4600 of raw materials cost unit,
4000 yuan/ton * 770kg=3080 of the production cost unit that increases
4600 yuan of-3080 yuan=1520 yuan/770kg of net income increase are equivalent to 1974 yuan of/ton crude drugs.
The inventive method
Reduce by 20000 yuan/ton * 500kg=10000 of raw materials cost unit.
4000 yuan/ton * 500kg=2000 of the production cost unit that increases
10000 yuan of-2000 yuan=8000 yuan/500kg of net income increase are equivalent to 16000 yuan of/ton crude drugs.
Make the utilization ratio of wild resource improve 1 times simultaneously, thereby can realize the protective development of wild Tibetan Chinese medicinal materials, and reduce the discharge capacity of pollutents such as the dregs of a decoction.
Embodiment
Providing embodiment below further specifies so that the present invention is made; but given embodiment can not be interpreted as limiting the scope of the invention, thereby nonessential improvement and adjustment that the professional and technical personnel has done according to the content and the design philosophy of the invention described above also should belong to protection scope of the present invention.
In addition, what deserves to be explained is that the used material umber of following examples is weight part.
Embodiment 1
Present embodiment is producing of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) mycelia.
1) natural wild red red-spotted stonecrop medicinal material is smashed to pieces, got 1g, add the 5ml sterilized water, concussion shakes up, and dilutes 10,100,1000 and 10000 times respectively with sterilized water again.Above each diluent is got 0.1ml respectively, coat in the isolation medium of forming by glucose 10g, peptone 5g, yeast extract paste 5g, potassium primary phosphate 1g, ammonium sulfate 0.5g, agar 20g and water 1000ml, cultivated 3 days through 30 ℃, picking list bacterium colony, be chosen in the Root of Kirilow Rhodiola substratum after the transformation fermentation, detect the bacterial strain that rhodioside content improves with high performance liquid chromatography (HPLC).
2) be that 80 purpose Root of Kirilow Rhodiola powder and water mix by 1: 10 weight part with particle diameter earlier, do not add 0.2% yeast extract paste again by the score of the weight part of water, 0.1% potassium primary phosphate and 0.05% sal epsom, be made into liquid seed culture medium, in liquid seed culture medium, insert aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) spore in the Aspergillus (Aspergillus) that the first step method obtained then respectively, cultivated 2 days at 30 ℃ of following shaking tables of temperature then, centrifugal back abandoning supernatant, collect the mycelia precipitation respectively, dry standby with whizzer.
Embodiment 2
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 60 order, add 0.5g yeast extract paste, 0.5g potassium primary phosphate, 0.2g sal epsom and 800mL water then, mix, make it to become the rhodiola rosea liquid aqueous suspensions, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 80g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 8: 2, gets final product in 50 hours 30 ℃ of heat insulating culture fermentations then.
The liquid conversion leavening that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.3586%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 85.96%.
Embodiment 3
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 80 order, add the water that 1g yeast extract paste, 1g potassium primary phosphate, 0.3g sal epsom and 900mL rise then, mix, make it to become the rhodiola rosea liquid aqueous suspensions, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 90g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 6: 4, gets final product in 50 hours 30 ℃ of heat insulating culture fermentations then.
The liquid conversion leavening that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.4125%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 93.33%.
Embodiment 4
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 80 order, add the water that 2g yeast extract paste, 2g potassium primary phosphate, 0.5g sal epsom and 1000mL rise then, mix, make it to become the rhodiola rosea liquid aqueous suspensions, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 100g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 4: 6, gets final product in 50 hours 30 ℃ of heat insulating culture fermentations then.
The liquid conversion leavening that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.3639%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 86.68%.
Embodiment 5
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 80 order, add the water that 1.2g yeast extract paste, 1.2g potassium primary phosphate, 0.3g sal epsom and 800mL rise then, mix, make it to become the rhodiola rosea liquid aqueous suspensions, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 40g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 2: 8, gets final product in 55 hours 28 ℃ of heat insulating culture fermentations then.
The liquid conversion leavening that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.3255%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 81.43%.
Embodiment 6
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 80 order, add the water that 1.5g yeast extract paste, 1.5g potassium primary phosphate, 0.3g sal epsom and 800mL rise then, mix, make it to become the rhodiola rosea liquid aqueous suspensions, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 60g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 5: 5, gets final product in 60 hours 32 ℃ of heat insulating culture fermentations then.
The liquid conversion leavening that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.3910%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 90.39%.
Embodiment 7
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 100 order, add the water that 2g yeast extract paste, 2g potassium primary phosphate, 0.4g sal epsom and 800mL rise then, mix, make it to become the rhodiola rosea liquid aqueous suspensions, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 120g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 3: 7, gets final product in 60 hours 30 ℃ of heat insulating culture fermentations then.
The liquid conversion leavening that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.5738%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 115.4
Embodiment 8
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 120 order, add the water that 1.5g yeast extract paste, 1.75g potassium primary phosphate, 0.25g sal epsom and 800mL rise then, mix, make it to become the rhodiola rosea liquid aqueous suspensions, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 160g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 7:3, gets final product in 48 hours 30 ℃ of heat insulating culture fermentations then.
The liquid conversion leavening that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.8194%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 149.03%.
Claims (5)
1. one kind prepares the method for rhodiola rosea liquid conversion leavening with microbial hybrid bacterial strain, and the processing step of this method and condition are:
1) the Root of Kirilow Rhodiola rhizome is crushed to 60～120 orders;
2) add 800～1000 parts of water in per 100 parts Root of Kirilow Rhodiola powder, 0.5～2 part of yeast extract paste, 0.5～2 part of potassium primary phosphate and 0.2～0.5 part of sal epsom mix, and make it to become the rhodiola rosea liquid aqueous suspensions, and sterilization;
3) in the liquid seed culture medium that contains the Root of Kirilow Rhodiola powder, prepare respectively in the Aspergillus aspergillus niger (Aspergillus niger) and the mycelium culture thing of aspergillus oryzae (Aspergillus oryzae);
The mycelium culture thing of two kinds of moulds that 4) will prepare will mix the mycelia culture and be inoculated in the rhodiola rosea liquid aqueous suspensions after mixing by weight 2～8: 8～2, and inoculum size is counted 5～20 parts of per 100 parts of water inoculations by weight in wet base;
5) postvaccinal prepared product was got final product in 28～32 ℃ of following heat insulating culture fermentations of temperature in 48～60 hours,
More than used material umber be weight part.
2. according to claim 1ly prepare the method for rhodiola rosea liquid conversion leavening with microbial hybrid bacterial strain, this method is that the Root of Kirilow Rhodiola rhizome is crushed to 80～120 orders.
3. the method for preparing rhodiola rosea liquid conversion leavening with microbial hybrid bacterial strain according to claim 1 and 2, this method is to add 800～900 parts of water in per 100 parts Root of Kirilow Rhodiola powder, 1～2 part of yeast extract paste, 1～2 part of potassium primary phosphate and 0.2～0.3 part of sal epsom, mix, make it to become the rhodiola rosea liquid aqueous suspensions.
4. the method for preparing rhodiola rosea liquid conversion leavening with microbial hybrid bacterial strain according to claim 1 and 2, the used mycelium culture thing of this method is to be that 80 purpose Root of Kirilow Rhodiola powder and water mix by 1: 10 weight part with particle diameter earlier, do not add 0.2% yeast extract paste again by the score of the weight part of water, 0.1% potassium primary phosphate and 0.05% sal epsom, be made into liquid seed culture medium, in liquid seed culture medium, insert the aspergillus niger (Aspergillus niger) in the Aspergillus (Aspergillus) and the spore of aspergillus oryzae (Aspergillus oryzae) then respectively, shaking table was cultivated 2 days under 30 ℃ of temperature, centrifugal back abandoning supernatant is collected the mycelia precipitation.
5. the method for preparing rhodiola rosea liquid conversion leavening with microbial hybrid bacterial strain according to claim 3, the used mycelium culture thing of this method is to be that 80 purpose Root of Kirilow Rhodiola powder and water mix by 1: 10 weight part with particle diameter earlier, do not add 0.2% yeast extract paste again by the score of the weight part of water, 0.1% potassium primary phosphate and 0.05% sal epsom, be made into liquid seed culture medium, in liquid seed culture medium, insert the aspergillus niger (Aspergillus niger) in the Aspergillus (Aspergillus) and the spore of aspergillus oryzae (Aspergillus oryzae) then respectively, cultivated 2 days at 30 ℃ of following shaking tables of temperature, centrifugal back abandoning supernatant is collected the mycelia precipitation.
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| KR101381112B1 (en) * | 2011-11-30 | 2014-04-14 | 한국식품연구원 | Preparation method for fermented rhodiola rosea using red ginseng and composition for fatigue recovery and improvement of exercise performance comprising the fermented rhodiola rosea |
| CN104257545B (en) * | 2014-09-18 | 2016-08-24 | 北京工商大学 | A kind of Radix Rhodiolae proferment pulp cosmetic and preparation method thereof |
| CN105861600A (en) * | 2016-04-13 | 2016-08-17 | 河南省商业科学研究所有限责任公司 | Preparation method of Rhodiola rosea extract for effectively promoting sleep and enhancing immunity |
| CN106265846A (en) * | 2016-09-18 | 2017-01-04 | 天津北洋百川生物技术有限公司 | A kind of fermentation process improving Radix Rhodiolae oxidation resistance |
| CN106399397A (en) * | 2016-09-18 | 2017-02-15 | 天津北洋百川生物技术有限公司 | Method for increasing content of tyrosol in rhodiola rosea by microorganism fermentation |
| CN107058431B (en) * | 2017-05-09 | 2020-10-27 | 王智森 | Method for producing salidroside by deep liquid fermentation of salidroside |
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