CN101717789B - Method for preparing culture medium for efficiently producing haematochrome - Google Patents
Method for preparing culture medium for efficiently producing haematochrome Download PDFInfo
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- CN101717789B CN101717789B CN2009102183523A CN200910218352A CN101717789B CN 101717789 B CN101717789 B CN 101717789B CN 2009102183523 A CN2009102183523 A CN 2009102183523A CN 200910218352 A CN200910218352 A CN 200910218352A CN 101717789 B CN101717789 B CN 101717789B
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- 239000001963 growth medium Substances 0.000 title abstract description 8
- 238000000034 method Methods 0.000 title description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 26
- 230000001580 bacterial effect Effects 0.000 claims abstract description 19
- 238000000855 fermentation Methods 0.000 claims abstract description 16
- 230000004151 fermentation Effects 0.000 claims abstract description 16
- 241000607715 Serratia marcescens Species 0.000 claims abstract description 15
- 235000011187 glycerol Nutrition 0.000 claims abstract description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 39
- 235000015097 nutrients Nutrition 0.000 claims description 34
- 108010046845 tryptones Proteins 0.000 claims description 25
- 239000001054 red pigment Substances 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 2
- 241000559219 Serratia sp. KMR-3 Species 0.000 claims 1
- 239000000049 pigment Substances 0.000 abstract description 27
- 239000012137 tryptone Substances 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000024053 secondary metabolic process Effects 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 32
- 241000894006 Bacteria Species 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- HCOLPNRPCMFHOH-UHFFFAOYSA-N Prodigiosin Natural products CCCCCC1C=C(C=C/2N=C(C=C2OC)c3ccc[nH]3)N=C1C HCOLPNRPCMFHOH-UHFFFAOYSA-N 0.000 description 7
- 241000607720 Serratia Species 0.000 description 7
- 241000607714 Serratia sp. Species 0.000 description 7
- 229940041514 candida albicans extract Drugs 0.000 description 7
- TWFGRJUTAULJPZ-USZBIXTISA-N prodigiosin Chemical compound N1=C(C)C(CCCCC)=C\C1=C/C1=NC(C=2[N]C=CC=2)=C[C]1OC TWFGRJUTAULJPZ-USZBIXTISA-N 0.000 description 7
- 239000012138 yeast extract Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 244000005700 microbiome Species 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 239000012533 medium component Substances 0.000 description 3
- 238000012803 optimization experiment Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 108010021006 Tyrothricin Proteins 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
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- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
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- 238000011156 evaluation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
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- 239000012092 media component Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- GSXRBRIWJGAPDU-BBVRJQLQSA-N tyrocidine A Chemical compound C([C@H]1C(=O)N[C@H](C(=O)N[C@@H](CCCN)C(=O)N[C@H](C(N[C@H](CC=2C=CC=CC=2)C(=O)N2CCC[C@H]2C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N1)=O)CC(C)C)C(C)C)C1=CC=C(O)C=C1 GSXRBRIWJGAPDU-BBVRJQLQSA-N 0.000 description 2
- 229960003281 tyrothricin Drugs 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000002177 Cataract Diseases 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 108010048581 Lysine decarboxylase Proteins 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-L Malonate Chemical compound [O-]C(=O)CC([O-])=O OFOBLEOULBTSOW-UHFFFAOYSA-L 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000052812 Ornithine decarboxylases Human genes 0.000 description 1
- 108700005126 Ornithine decarboxylases Proteins 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- FBPFZTCFMRRESA-GUCUJZIJSA-N galactitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-GUCUJZIJSA-N 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention discloses a culture medium for producing haematochrome by serratia marcescens, which is a culture medium using tryptone as a nitrogen source. The culture medium is prepared from tryptone, glycerine and water. The culture medium not only has the characteristics of safety and little protein using amount, but also promotes the serratia marcescens to quickly produce a large amount of haematochrome, and is applied in the fields of bacterial secondary metabolism product fermentation, laboratory culture medium, natural pigment development and the like in modern bioengineering.
Description
Technical field
The present invention relates to a kind of chromatogenous substratum of serratia marcescens that is used for, particularly a kind of with the preparation method of Tryptones as the substratum of only nitrogen source.
Background technology
A lot of natural pigments have safe and reliable, do not have toxic side effect, the tone nature; Near crude substance, a lot of kinds all have advantages such as physiologically active, are that the ubiquity of occurring in nature also is the most stable natural pigment like β-Hu Luobusu; Be inhibitor, have detoxification, on anticancer, preventing cardiovascular disease and cataract, remarkable function is arranged; And prevent to wear out and the old and feeble multiple degenerative disorders that causes; In addition, promoting aspect animal fertility and the growth better effect is arranged also, therefore developing natural pigment is the emphasis of studying in the world.Therefore utilize Microbial resources fermentative prodn natural pigment to open up wide field for people in recent years, but far can not satisfy industrial needs, screening is produced the mikrobe that pigment especially has a pharmaceutical use and is had broad prospects from natural resources.
Screening obtains the tyrothricin that red pigment is produced in a strain from the refinery effluent of Kunming, shows that by the sequential analysis of Physiology and biochemistry experiment and 16S rDNA this tyrothricin is a serratia marcescens, is starting strain with this bacterial strain, and fermentation condition of its product pigment is studied.Serratia marcescens is claimed Bacterium prodigiosum again, is reckling in the bacterium, and whole body flagellum can move, and no pod membrane, no gemma, about half bacterial strain can produce red prodigiosin (prodigiosins).Prodigiosin is one type of natural red colouring matter, has certain immunocompetence, as suppressing fungi, bacterium and mould etc., certain antitumor action is arranged also, and therefore very big pharmaceutical use is arranged.
Summary of the invention
The present invention is intended to that a kind of safety is provided, the albumen consumption is few, cost is low and promote serratia marcescens (Serratia sp.) KMR-3 bacterial strain to produce the liquid nutrient medium of a large amount of red pigment fast.
Realize that the object of the invention technical scheme is a kind of liquid nutrient medium, it is that the component of substratum is formulated with Tryptones and water.
Further technical scheme; A kind of serratia marcescens KMR-3 strain fermentation that is used for produces the preparation method of red pigment with liquid nutrient medium, is to be the component of substratum with Tryptones and water, with the LB liquid nutrient medium as initial substratum; It is characterized in that: this substratum is to be only nitrogen source with the Tryptones; And contain glycerine in the composition of substratum, leavening temperature is 15 ℃～28 ℃, and the pH value is 4～10.
The content of Tryptones is 5g/L in the described substratum, and the content of glycerine is 1%.
Said leavening temperature is 28 ℃, and fermentation time is 36h, and the pH value of LB liquid nutrient medium is 6.0.
Contain glycerine in the said substratum, be used to improve the content that secondary metabolite is a red pigment.
Serratia marcescens (Serratia sp.) the KMR-3 bacterial strain of red pigment is produced in the strain that utilization of the present invention is separated to the waste water around the smeltery, Kunming, and its preserving number is: CGMCC No.2636.Through optimizing the fermentation condition and the medium component of substratum, utilize the red pigment that has obtained high yield with Tryptones as the substratum of only nitrogen source.
The serratia marcescens that the present invention relates to (Serratia sp.) KMR-3 bacterial strain on August 26th, 2008 in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center " (BeiJing, China) preservation; preserving number is: CGMCC No.2636, and declared patent of invention in 2008.
Concrete technological step of the present invention is following: LB (Luria-Bertani) substratum is a most frequently used substratum in the microbiology experiment, is used to cultivate bacterium such as intestinal bacteria and is divided into liquid state or adds the solid medium that agar is processed.Produce the initial substratum of red pigment as serratia marcescens (Serratia sp.) KMR-3 strain fermentation with the LB liquid nutrient medium; Leavening temperature is 25 ℃; The pH value is 6.0; (NaCl10g/L) heterogeneity improves the output of this bacterial strain secondary metabolite red pigment for Tryptones 10g/L, yeast extract paste 5g/L through optimizing the LB liquid nutrient medium.The initial optimization experiment content is: the LB liquid nutrient medium, do not contain the improvement LB liquid nutrient medium of Tryptones, and do not contain the improvement LB liquid nutrient medium of yeast extract paste, do not contain the improvement LB liquid nutrient medium of NaCl; Through measuring the centrifugal supernatant OD of fermented liquid
535Value is estimated pigment production, shows that the improvement liquid nutrient medium that does not contain yeast extract paste and different N aCl all can improve pigment production (Fig. 1) to a certain extent.Result according to initial optimization selects for use the substratum that only contains the different concns Tryptones to ferment; The effect better (Fig. 2) that shows the 5g/L Tryptones; In substratum, add 1% glycerine then and be used for further improving the output of pigment; Find effect best (Fig. 2), therefore, confirm that finally the medium component of high yield pigment is: 5g/L Tryptones and 1% glycerine.
Serratia marcescens is claimed Bacterium prodigiosum again, is reckling in the bacterium, and about half bacterial strain can produce red prodigiosin (prodigiosins).Prodigiosin is one type of natural red colouring matter; Have antibacterium, fungi, malaria and mould, immunosuppression and effect such as antitumor; And the prodigiosin of microbial fermentation production still is more satisfactory natural pigment, can be used for foodstuffs industry in a large number, so this pigment has good application prospects.Along with modern biotechnology develop rapidly and to the further investigation of prodigiosin, the generation of prodigiosin and mechanism of action will be by understanding more clearly, a large amount of preparations, and be applied to immunotherapy to bring benefit to the mankind.
Description of drawings
Below in conjunction with accompanying drawing, further specify essentiality content of the present invention with embodiment, but do not limit the present invention with this.
The different nutrient media components KMR-3 of the different incubation times of Fig. 1 the present invention bacterial strain produces amount of pigment;
The different nutrient media components KMR-3 of the different incubation times of Fig. 2 the present invention bacterial strain produces amount of pigment.
Embodiment
Embodiment 1:
Serratia (Serratia sp.) KMR-3 bacterial strain physiological and biochemical property:
A strain that is separated to the waste water around the smeltery, Kunming has high resistance to cupric ion serratia (Serratia sp.) KMR-3 bacterial strain.This serratia (Serratia sp.) KMR-3 bacterial strain on August 26th, 2008 in that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center " (BeiJing, China) preservation, preserving number is: CGMCC No.2636.
KMR-3 bacterial strain physiological and biochemical property: KMR-3 strain growth temperature and pH pH-value determination pH; The KMR-3 bacterium liquid of coating 10ml fresh culture is on the LB solid plate; Put 4 ℃, 15 ℃, 28 ℃, 37 ℃ and 42 ℃ of cultivations respectively, observe the influence that bacterial growth situation and temperature produce pigment.The KMR-3 bacterium liquid of inoculation 10ml fresh culture is in the 5mL LB of different pH (1～14) liquid nutrient medium, and 28 ℃, 150rpm cultivates 24h, observes thalli growth pH scope.The result shows: the KMR-3 bacterial strain can be grown under 4 ℃, 15 ℃, 28 ℃, 37 ℃ culture condition, does not grow at 42 ℃; 15 ℃～28 ℃ cultivate to produce red pigments, but 37 ℃ cultivated not chromogenesis, can produce red pigments and be reduced to 28 ℃ of cultivations to culture temperature from 37 ℃, and the characteristic of this and Serratia is very close.The KMR-3 bacterial strain can be grown in the LB liquid nutrient medium of pH4～10 scopes, and all produces red pigments, and wherein growth is the fastest near pH6.0, tentatively regards as the righttest growth pH of this bacterium.
According to known data, there are 3 kinds can produce red pigments-prodigiosin in the serratia, be respectively serratia marcescens, serratia rubida and general city Serratia.Through further physiological and biochemical property it is accredited as serratia marcescens: the KMR-3 bacterial strain can be grown as sole carbon source with glucose, sorbyl alcohol, Pentitol, malonate and western Meng Shi citrate; Can not grow as sole carbon source with lactose and galactitol; Urea can not be utilized, H can not be produced
2S, lysine decarboxylase, ornithine decarboxylase and PD reaction are positive.
Embodiment 2:
The optimization of medium component:
The initial optimization of the LB liquid nutrient medium of improvement: (NaCl10g/L) as initial substratum, leavening temperature is 25 ℃ for Tryptones 10g/L, yeast extract paste 5g/L, and the pH value is 6.0 with the LB liquid nutrient medium.The initial optimization experiment content is: the LB liquid nutrient medium, do not contain the improvement LB liquid nutrient medium of Tryptones, and do not contain the improvement LB liquid nutrient medium of yeast extract paste, do not contain the improvement LB liquid nutrient medium of NaCl; Respectively at 0,6,12; 24,36,48; 60,72 take a sample to fermented liquid with 90h, measure OD through the centrifuging and taking supernatant
535Value entry evaluation pigment production (Fig. 1).The result shows: during fermentation 12h, the improvement LB liquid nutrient medium product pigment ability that does not contain NaCl is stronger, OD
535Value is 0.032; And that the improvement LB liquid nutrient medium that does not contain yeast extract paste when fermenting to 48h produces the pigment ability is the strongest, OD
535Value is 0.135.Therefore the substratum that adopts the improvement liquid nutrient medium that does not contain yeast extract paste and NaCl promptly only to contain the different concns Tryptones carries out the fermentation optimization experiment.
Different concns Tryptones Optimum of culture medium: the liquid nutrient medium only to contain Tryptones (5g/L, 10g/L and 15g/L) ferments, and culture temperature is 25 ℃, and the pH value is 6.0.Respectively at 0,6,12,24,36,48,60 take a sample to fermented liquid with 72h, measure OD through the centrifuging and taking supernatant
535Value entry evaluation pigment production (Fig. 2).The result shows: during fermentation 36h, and the OD of initial substratum (LB)
535Value is 0.266, and contains the liquid nutrient medium of 5g/L Tryptones, OD
535Value is 0.557, produces the pigment ability and improves 2 times, is lower than initial substratum but the liquid nutrient medium of 10g/L and 15g/L Tryptones produces the pigment ability.According to document announcement, glycerine has the effect that improves pigment production, therefore adopts the method that adds glycerine further to optimize substratum to improve pigment production.
The optimization of Tryptones and glycerin medium: the liquid nutrient medium with 5g/L Tryptones and 1% glycerine ferments, and culture temperature is 25 ℃, and the pH value is 6.0.Respectively at 0,6,12,24,36,48,60 take a sample to fermented liquid with 72h, measure OD through the centrifuging and taking supernatant
535Value assessment pigment production (Fig. 2).The result shows: during fermentation 36h, this substratum produces the pigment ability and is higher than the liquid nutrient medium that only contains Tryptones (5g/L, 10g/L and 15g/L), OD far away
535Value is 3.565, and the fermentation of initial LB liquid nutrient medium: OD during fermentation 36h
535Value is merely 0.266, and 5g/L Tryptones of therefore optimizing and the liquid nutrient medium of 1% glycerine product pigment ability are 13.4 times of initial substratum.
Claims (2)
1. one kind is used for serratia marcescens KMR-3 bacterial strain (Serratia sp.KMR-3) fermentation and produces red pigment and use liquid nutrient medium; Substratum is to be the component of substratum with Tryptones and water; It is characterized in that: this substratum is to be only nitrogen source with the Tryptones; And contain glycerine in the composition of substratum, leavening temperature is 15 ℃～28 ℃, and the pH value is 4～10;
The content of Tryptones is 5g/L in the described substratum, and the content of glycerine is 1%.
2. a kind of serratia marcescens KMR-3 strain fermentation generation red pigment that is used for according to claim 1 is used liquid nutrient medium, and it is characterized in that: said this substratum leavening temperature is 28 ℃, and fermentation time is 36h, and the pH value of substratum is 6.0.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110564644B (en) * | 2019-09-17 | 2021-04-02 | 山东农业大学 | Serratia atropurpurea with growth promoting effect and application thereof |
| CN111621436B (en) * | 2020-06-02 | 2022-05-13 | 四川省农业科学院土壤肥料研究所 | Culture solution suitable for producing prodigiosin by Serratia marcescens XD1-B-1 |
| CN112159786B (en) * | 2020-11-04 | 2022-03-01 | 河北科技大学 | Cr (VI) reducing strain C6, and culture condition and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4266028A (en) * | 1979-02-21 | 1981-05-05 | Kirin Beer Kabushiki Kaisha | Process for preparation of prodigiosin |
| CN1563400A (en) * | 2004-04-19 | 2005-01-12 | 华东理工大学 | Method for separating and preparing prodigiosin |
| CN101392227A (en) * | 2008-10-23 | 2009-03-25 | 新疆大学 | Bacillus prodigiosus and prodigiosin producted thereby |
| CN101509000A (en) * | 2008-11-24 | 2009-08-19 | 昆明理工大学 | Bacteria copper resistant gene, recombinant vector containing the same, preparation and expression thereof |
-
2009
- 2009-12-14 CN CN2009102183523A patent/CN101717789B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4266028A (en) * | 1979-02-21 | 1981-05-05 | Kirin Beer Kabushiki Kaisha | Process for preparation of prodigiosin |
| CN1563400A (en) * | 2004-04-19 | 2005-01-12 | 华东理工大学 | Method for separating and preparing prodigiosin |
| CN101392227A (en) * | 2008-10-23 | 2009-03-25 | 新疆大学 | Bacillus prodigiosus and prodigiosin producted thereby |
| CN101509000A (en) * | 2008-11-24 | 2009-08-19 | 昆明理工大学 | Bacteria copper resistant gene, recombinant vector containing the same, preparation and expression thereof |
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