CN101413013A - Method for preparing Rhodiola rosea solid-state transformation fermentate by using microbial mixing bacterial strain - Google Patents
Method for preparing Rhodiola rosea solid-state transformation fermentate by using microbial mixing bacterial strain Download PDFInfo
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- CN101413013A CN101413013A CNA2008100463686A CN200810046368A CN101413013A CN 101413013 A CN101413013 A CN 101413013A CN A2008100463686 A CNA2008100463686 A CN A2008100463686A CN 200810046368 A CN200810046368 A CN 200810046368A CN 101413013 A CN101413013 A CN 101413013A
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Abstract
The invention provides a method for improving active ingredients in a rhodiola rosae raw medicinal material through the transformation fermentation by a mixed microorganism strain in a solid culture material of the rhodiola rosae raw medicinal material. The method comprises the following steps: the solid culture material of rhodiola rosae produced by rootstalk powder of the rhodiola rosae is sterilized and then is grafted with the mixed microorganism strain for solid fermentation culture. Through the transformation fermentation of the method, the active ingredients of the rhodiola rosae can be greatly improved than those of the raw medicinal material to further ensure that the content of the active ingredients is improved by more than 70 percent than that of the raw medicinal material at best, so the method has the advantages that the method can effectively utilize wild resources, can also reduce the discharge amount of pollutants such as medicine residues and so on at the same time, realize the protective development of traditional Tibetan medicines, and has good application prospect.
Description
Technical field
The invention belongs to from Root of Kirilow Rhodiola and hide the technical field of extracting the effective active composition the Chinese medicinal materials, be specifically related to a kind ofly prepare the method for Rhodiola rosea solid-state transformation fermentate with microbial hybrid bacterial strain, the effective active composition in this fermented product improves greatly.
Background technology
Root of Kirilow Rhodiola is meant the Crassulaceae Rhodida plant, have 90 in the world surplus kind, be distributed in the severe cold areas in the Northern Hemisphere more, great majority all are grown under the high mountain flowstones or bushes about height above sea level 3500-5000 rice.China has now found 73 kinds, mainly is distributed in the high mountain severe cold areas in China Tibet, and also there is the Root of Kirilow Rhodiola resource distribution in some areas in northeast, northwest, North China in addition.
The main medicinal part of Root of Kirilow Rhodiola is a rhizome, wherein contained effective constituent rhodioloside and the tyrosol (glucoside unit) of mainly containing, have effects such as antifatigue, anti-hypoxia, antimutagenic and inhibition tumour, and (Song Yueying etc. have no side effect, Rhodida plant chemical ingredients and Pharmacological action study progress herbal medicine .2004,35 (2) .-235-236; Li Gang waits the pharmaceutical research of Tibetan medicine Root of Kirilow Rhodiola to make progress Chinese national medicine magazine .2004,10 (3) .-38-40; The Advance on Pharmacological Activities Chinese materia medica journal .2003 of the pious Root of Kirilow Rhodiola of the flat Wang Sheng of the bright Yan Ji of king family, 31 (4) .-57-59), therefore, in the exploitation of medicine and protective foods, obtained using widely.
Owing to, make Tibetan area, this plateau resources of natural crude drugs face exhausted crisis to a large amount of development and use of Root of Kirilow Rhodiola.And the Root of Kirilow Rhodiola activeconstituents preparation technology who adopts at present directly soaks the Root of Kirilow Rhodiola raw material with alcohols (ethanol etc.) to extract.This method can only be extracted wherein the effective active composition that exists, and effective active component content wherein is very low, is about 0.6～0.7% as the content of rhodioside.In the face of a large amount of social demands, how on the basis of current extraction method, to develop new preparation method, to improve the utilization ratio of Root of Kirilow Rhodiola crude drug, become current this area urgent problem.The new process of having reported has chemical synthesis, plant cell tissue's culture method and with the microbe transformation method of single bacterial strain.These methods all are before the alcohols extraction process, and desire obtains by different sources or increases effective constituent, and expectation reduces the consumption of wild resource.Wherein microbe transformation method is the method for tool application prospect from the effective constituent that improves Root of Kirilow Rhodiola itself, is that employing single microorganism bacterial strain carries out transformation fermentation as the disclosed technology of patent application " a kind of method of preparing rhodiola root extract transformed by microbe " (application number 200610146107.2).Though this method can improve the effective constituent of Root of Kirilow Rhodiola itself, can only improve the effective active component content because of it is the highest about 30%, so improve limited, desirable not enough.
Summary of the invention
The objective of the invention is to carry out transformation fermentation and improve the defective that the rhodiola active ingredient method exists at existing employing single microorganism bacterial strain, a kind of method for preparing Rhodiola rosea solid-state transformation fermentate with microbial hybrid bacterial strain is provided, this method can significantly improve effective active composition in the Root of Kirilow Rhodiola crude drug, under the condition of utilizing the isodose crude drug, can obtain the more product that extracts, wild Tibetan Chinese medicinal materials is fully used.
Provided by the inventionly prepare the method for rhodiola liquid solid-state transformation fermentate with microbial hybrid bacterial strain, the processing step of this method and condition are:
1) the Root of Kirilow Rhodiola rhizome is crushed to 60～120 orders, preferred 80～120 orders, more preferably 80 orders;
2) in per 100 parts Root of Kirilow Rhodiola powder, add 80～120 parts of water, preferred 90～100 parts, more preferably 100 parts, 0.05～0.2 part of yeast extract paste, preferred 0.1～0.2 part, more preferably 0.2 part, 0.05～0.2 part of potassium primary phosphate, preferred 0.1～0.2 part, more preferably 0.2 part, with 0.02～0.05 part of sal epsom, preferred 0.03～0.04 part, more preferably 0.03 part, mix, make it to become the Rhodiola rosea solid-state culture material, and sterilization;
3) in Root of Kirilow Rhodiola wheat bran seed culture medium, prepare the aspergillus niger (Aspergillus niger) in the Aspergillus and the mycelium culture thing of aspergillus oryzae (Aspergillus oryzae) respectively;
The mycelium culture thing of two kinds of moulds that 4) will prepare will mix the mycelia culture again and be inoculated in the Rhodiola rosea solid-state culture material after mixing by weight 2～8: 8～2, and inoculum size is by 5～20 parts (weight in wet base) of per 100 parts of Root of Kirilow Rhodiola powder inoculation;
5) postvaccinal prepared product was fermented 48～60 hours in 28～32 ℃ of following heat insulating culture of temperature, 30 ℃ of preferred temperature, incubation time got final product in 60 hours,
More than the weight in wet base of Jie Zhong mycelium culture thing is the gross weight of cultivating the mycelium culture thing natural water-bearing after 2 days.
In this method the Rhodiola rosea solid-state culture material is sterilized and can be carried out under normal condition, as sterilizing 30 minutes for 121 ℃ in temperature.
Used mycelium culture thing is to cultivate like this in this method: be earlier 80 purpose Root of Kirilow Rhodiola powder, wheat bran with particle diameter with water mixed in 0.8: 0.2: 1 by weight mix thoroughly after, steam sterilizing promptly becomes Root of Kirilow Rhodiola wheat bran seed culture medium.In Root of Kirilow Rhodiola wheat bran seed culture medium, insert the aspergillus niger (Aspergillus niger) in the Aspergillus (Aspergillus) and the spore of aspergillus oryzae (Aspergillus oryzae) then respectively, under 30 ℃ of temperature, cultivated 2 days, promptly become the mycelium culture thing.
Two kinds of wherein used moulds both can have been adopted aspergillus niger (Aspergillus niger) and the aspergillus oryzae (Aspergillus oryzae) in the existing Aspergillus (Aspergillus), also can adopt the inventor to obtain after separation and purification and the screening from the wild red red-spotted stonecrop medicinal material, preferred back is a kind of.The inventor from wild red red-spotted stonecrop medicinal material after separation and purification and the screening aspergillus niger (Aspergillus niger) that obtains and the concrete grammar of aspergillus oryzae (Aspergillus oryzae) be: natural wild red red-spotted stonecrop medicinal material is smashed to pieces, get 1g, add the 5ml sterilized water, concussion shakes up, and dilutes 10,100,1000 and 10000 times respectively with sterilized water again.Above each diluent is got 0.1ml respectively, coat in the isolation medium, cultivated 3 days through 30 ℃, picking list bacterium colony is chosen in the Root of Kirilow Rhodiola substratum after the transformation fermentation, detects the bacterial strain that rhodioside content improves with high performance liquid chromatography (HPLC).The separation and Culture based formulas is: glucose 10g, peptone 5g, yeast extract paste 5g, potassium primary phosphate 1g, ammonium sulfate 0.5g, agar 20g and water 1000ml.Isolated two kinds of moulds be accredited as aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) in the Aspergillus (Aspergillus) according to conventional methods.
For the main effectively content of activeconstituents rhodioside in the rhodiola plant, the content of rhodioside answers 〉=0.5% in a regulation of Chinese Pharmacopoeia version in 2005 medicinal material, and the content of rhodioside is about 0.6～0.7% in actual measurement neutral red red-spotted stonecrop crude drug.The preparation technology of existing rhodioside extract adopts alcohols (ethanol etc.) directly to soak to extract, so can only extract the rhodioside that has existed.Because the main effectively activeconstituents rhodioloside of Root of Kirilow Rhodiola and the synthetic secondary metabolism that belongs to of tyrosol (glucoside unit), tyrosol and rhodioloside are to add respectively that on the phenol architecture basics ethanol based and glucosyl group form.The inventor is when the main effectively activeconstituents rhodioloside of studying Root of Kirilow Rhodiola and tyrosol (glucoside unit), find to exist certain transformable precursor in the rhodiola plant, thereby have abundant metabolism diversity and very strong metabolic capacity according to microorganism, and has a pathways metabolism that in the higher plant body, exists, once the enzyme that had proposed with microorganism is the precursor that comes non-activity in the catalysis Root of Kirilow Rhodiola raw material, make it and synthetic tyrosol of compositions such as glucosyl group and ethanol based and rhodioloside, to improve the technical scheme (number of patent application 200610146107.2) of rhodioloside and tyrosol (glucoside unit) content.The principle of this technical scheme as shown in drawings.
But because this method employing is single bacterial strain, though can improve the content of effective active composition, its raising amount is comparatively limited.The inventor is in further studying, courageously break the inertial thinking of " it is poorer than single enzyme effect to use mixed enzyme to ferment ", carried out creationary exploration, the above-mentioned method for preparing rhodiola rosea liquid conversion leavening with the aspergillus niger in the Aspergillus (Aspergillus niger) and two kinds of microbial hybrid bacterial strains of aspergillus oryzae (Aspergillus oryzae) is provided.This method can significantly improve effective active composition in the Root of Kirilow Rhodiola crude drug, make the highest comparable crude drug of content of rhodioside improve more than 70%, thereby under the condition of utilizing the isodose crude drug, can obtain more to extract product, wild Tibetan Chinese medicinal materials is fully used.
Because the highest comparable crude drug of the content of rhodioside improves more than 70% in the transformation fermentation thing of gained after the inventive method fermentative processing, thereby when obtaining the isodose product, can reduce crude drug consumption.And can find out in the analytical calculation by following economic benefit that also the synergy of single strain conversion method is near the breakeven point; And present method synergy is 4.7 times of the single strain conversion method, has very strong application prospect:
Press the equal cubage of rhodioside, directly use 1 ton of the former processes expend crude drug of alcohol immersion, the single strain conversion method needs crude drug 770kg (calculating by improving 30%), and the inventive method only needs crude drug 600kg (calculating by improving 70%).Identical because of the single strain conversion method again with the production cost that the inventive method increases, about 4000 yuan of/ton crude drugs.By 20,000 yuan of crude drugs per ton,
The single strain conversion method
Reduce by 20000 yuan/ton * 230kg=4600 of raw materials cost unit,
4000 yuan/ton * 770kg=3080 of the production cost unit that increases
4600 yuan of-3080 yuan=1520 yuan/770kg of net income increase are equivalent to 1974 yuan of/ton crude drugs.
The inventive method
Reduce by 20000 yuan/ton * 400kg=8000 of raw materials cost unit.
4000 yuan/ton * 600kg=2400 of the production cost unit that increases
8000 yuan of-2400 yuan=5600 yuan/600kg of net income increase are equivalent to 9333 yuan of/ton crude drugs.
Make the utilization ratio of wild resource improve 60% simultaneously, thereby can realize the protective development of wild Tibetan Chinese medicinal materials, and reduce the discharge capacity of pollutents such as the dregs of a decoction.
Description of drawings
Accompanying drawing is the schematic diagram of technical solution of the present invention.
Embodiment
Providing embodiment below further specifies so that the present invention is made; but given embodiment can not be interpreted as limiting the scope of the invention, thereby nonessential improvement and adjustment that the professional and technical personnel has done according to the content of the invention described above also should belong to protection scope of the present invention.
In addition, what deserves to be explained is that the used material umber of following examples is weight part.
Embodiment 1
Present embodiment is producing of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) mycelia.
1) natural wild red red-spotted stonecrop medicinal material is smashed to pieces, got 1g, add the 5ml sterilized water, concussion shakes up, and dilutes 10,100,1000 and 10000 times respectively with sterilized water again.Above each diluent is got 0.1ml respectively, coat in the isolation medium of forming by glucose 10g, peptone 5g, yeast extract paste 5g, potassium primary phosphate 1g, ammonium sulfate 0.5g, agar 20g and water 1000ml, cultivated 3 days through 30 ℃, picking list bacterium colony, be chosen in the Root of Kirilow Rhodiola substratum after the transformation fermentation, detect the bacterial strain that rhodioside content improves with high performance liquid chromatography (HPLC).
2) earlier be 80 purpose Root of Kirilow Rhodiola powder, wheat bran with particle diameter with water mixed in 0.8: 0.2: 1 by weight mix thoroughly after, steam sterilizing promptly obtains Root of Kirilow Rhodiola wheat bran seed culture medium, insert aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) spore in the Aspergillus (Aspergillus) then therein respectively, shaking table was cultivated 2 days under 30 ℃ of temperature, and is standby.
Embodiment 2
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 80 order, add 0.05g yeast extract paste, 0.05g potassium primary phosphate, 0.02g sal epsom and 100mL water then, mix, make it to become the Rhodiola rosea solid-state culture material, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 5g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 8: 2, gets final product in 60 hours 28 ℃ of heat insulating culture fermentations then.
The solid-state transformation fermentate that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.1869%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 62.46%.
Embodiment 3
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 80 order, add 0.1g yeast extract paste, 0.1g potassium primary phosphate, 0.03g sal epsom and 100mL water then, mix, make it to become the Rhodiola rosea solid-state culture material, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 7.5g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 6: 4, gets final product in 55 hours 30 ℃ of heat insulating culture fermentations then.
The solid-state transformation fermentate that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.2469%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 70.67%.
Embodiment 4
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 80 order, add 0.15g yeast extract paste, 0.15g potassium primary phosphate, 0.04g sal epsom and 100mL water then, mix, make it to become the Rhodiola rosea solid-state culture material, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 10g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 4: 6, gets final product in 50 hours 30 ℃ of heat insulating culture fermentations then.
The solid-state transformation fermentate that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.2569%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 72.04%.
Embodiment 5
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 80 order, add 0.2g yeast extract paste, 0.2g potassium primary phosphate, 0.05g sal epsom and 100mL water then, mix, make it to become the Rhodiola rosea solid-state culture material, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 15g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 2: 8, gets final product in 48 hours 32 ℃ of heat insulating culture fermentations then.
The solid-state transformation fermentate that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.2348%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 69.01%.
Embodiment 6
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 60 order, add 0.2g yeast extract paste, 0.2g potassium primary phosphate, 0.03g sal epsom and 120mL water then, mix, make it to become the Rhodiola rosea solid-state culture material, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 20g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 5: 5, gets final product in 60 hours 30 ℃ of heat insulating culture fermentations then.
The solid-state transformation fermentate that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.2852%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 75.91%.
Embodiment 7
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 100 order, add 0.2g yeast extract paste, 0.2g potassium primary phosphate, 0.03g sal epsom and 90mL water then, mix, make it to become the Rhodiola rosea solid-state culture material, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 18g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 3: 7, gets final product in 60 hours 30 ℃ of heat insulating culture fermentations then.
The solid-state transformation fermentate that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.3235%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 81.15%.
Embodiment 8
With 100g Root of Kirilow Rhodiola rhizome raw material pulverizing to 120 order, add 0.2g yeast extract paste, 0.2g potassium primary phosphate, 0.03g sal epsom and 80mL water then, mix, make it to become the Rhodiola rosea solid-state culture material, and sterilized 30 minutes down at 121 ℃ with steam; The mixing fungal hyphae body of the 16g (weight in wet base) of embodiment 1 method preparation is pressed in the inoculation of cooling back, wherein the weight proportion of aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae) is 7: 3, gets final product in 60 hours 30 ℃ of heat insulating culture fermentations then.
The solid-state transformation fermentate that obtains is detected with high performance liquid chromatography (HPLC), rhodioside content is 1.3008%, the rhodioside content of the original medicinal material that detects with similarity condition is 0.7306% to compare, and the rhodioside content in transforming the secondary fermentation thing can improve 79.05%.
Claims (5)
1, a kind ofly prepare the method for Rhodiola rosea solid-state transformation fermentate with microbial hybrid bacterial strain, the processing step of this method and condition are:
1) the Root of Kirilow Rhodiola rhizome is crushed to 60～120 orders;
2) add 80～120 parts of water in per 100 parts Root of Kirilow Rhodiola powder, 0.05～0.2 part of yeast extract paste, 0.05～0.2 part of potassium primary phosphate and 0.02～0.05 part of sal epsom mix, and make it to become the Rhodiola rosea solid-state culture material, and sterilization;
3) in Root of Kirilow Rhodiola wheat bran seed culture medium, prepare respectively in the Aspergillus aspergillus niger (Aspergillusniger) and the mycelium culture thing of aspergillus oryzae (Aspergillus oryzae);
The mycelium culture thing of two kinds of moulds that 4) will prepare will mix the mycelia culture again and be inoculated in the Rhodiola rosea solid-state culture material after mixing by weight 2～8: 8～2, and inoculum size is by 5～20 parts (weight in wet base) of per 100 parts of Root of Kirilow Rhodiola powder inoculation;
5) postvaccinal prepared product was got final product in 28～32 ℃ of following heat insulating culture fermentations of temperature in 48～60 hours,
More than used material umber be weight part.
2, according to claim 1ly prepare the method for Rhodiola rosea solid-state transformation fermentate with microbial hybrid bacterial strain, this method is that the Root of Kirilow Rhodiola rhizome is crushed to 80～120 orders.
3, the method for preparing Rhodiola rosea solid-state transformation fermentate with microbial hybrid bacterial strain according to claim 1 and 2, this method is to add 90～100 parts of water in per 100 parts Root of Kirilow Rhodiola powder, 0.1～0.2 part of yeast extract paste, 0.1～0.2 part of potassium primary phosphate and 0.03～0.04 part of sal epsom, mix, make it to become the Rhodiola rosea solid-state culture material.
4, the method for preparing Rhodiola rosea solid-state transformation fermentate with microbial hybrid bacterial strain according to claim 1 and 2, the used mycelium culture thing of this method is earlier be 80 purpose Root of Kirilow Rhodiola powder, wheat bran with particle diameter with water mixed in 0.8: 0.2: 1 by weight mix thoroughly after, steam sterilizing becomes Root of Kirilow Rhodiola wheat bran seed culture medium.In this Root of Kirilow Rhodiola wheat bran seed culture medium, insert the aspergillus niger (Aspergillus niger) in the Aspergillus (Aspergillus) and the spore of aspergillus oryzae (Aspergillus oryzae) then respectively, under 30 ℃ of temperature, cultivated 2 days, promptly become the mycelium culture thing.
5, the method for preparing Rhodiola rosea solid-state transformation fermentate with microbial hybrid bacterial strain according to claim 3, the used mycelium culture thing of this method is earlier be 80 purpose Root of Kirilow Rhodiola powder, wheat bran with particle diameter with water mixed in 0.8: 0.2: 1 by weight mix thoroughly after, steam sterilizing becomes Root of Kirilow Rhodiola wheat bran seed culture medium.In this Root of Kirilow Rhodiola bran mass, insert the aspergillus niger (Aspergillus niger) in the Aspergillus (Aspergillus) and the spore of aspergillus oryzae (Aspergillus oryzae) then respectively, under 30 ℃ of temperature, cultivated 2 days, promptly become the mycelium culture thing.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861600A (en) * | 2016-04-13 | 2016-08-17 | 河南省商业科学研究所有限责任公司 | Preparation method of Rhodiola rosea extract for effectively promoting sleep and enhancing immunity |
| CN105943571A (en) * | 2016-07-13 | 2016-09-21 | 秦小波 | Preparation method and application of anti-tumor kiwi fruit waste extract |
| CN107058431A (en) * | 2017-05-09 | 2017-08-18 | 王智森 | A kind of method that rhodiola root liquid deep layer fermenting produces rhodioside |
| CN107164413A (en) * | 2017-06-30 | 2017-09-15 | 北京农学院 | The purposes of Yupingfeng dregs of a decoction tunning |
-
2008
- 2008-10-24 CN CNA2008100463686A patent/CN101413013A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861600A (en) * | 2016-04-13 | 2016-08-17 | 河南省商业科学研究所有限责任公司 | Preparation method of Rhodiola rosea extract for effectively promoting sleep and enhancing immunity |
| CN105943571A (en) * | 2016-07-13 | 2016-09-21 | 秦小波 | Preparation method and application of anti-tumor kiwi fruit waste extract |
| CN105943571B (en) * | 2016-07-13 | 2020-06-02 | 秦小波 | Preparation method and application of kiwifruit waste anti-tumor extract |
| CN107058431A (en) * | 2017-05-09 | 2017-08-18 | 王智森 | A kind of method that rhodiola root liquid deep layer fermenting produces rhodioside |
| CN107164413A (en) * | 2017-06-30 | 2017-09-15 | 北京农学院 | The purposes of Yupingfeng dregs of a decoction tunning |
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