CN114350526A - Method for improving monascus pigment tone - Google Patents
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- CN114350526A CN114350526A CN202210040184.9A CN202210040184A CN114350526A CN 114350526 A CN114350526 A CN 114350526A CN 202210040184 A CN202210040184 A CN 202210040184A CN 114350526 A CN114350526 A CN 114350526A
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- monascus
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- lauryl ether
- polyoxyethylene lauryl
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- 241000228347 Monascus <ascomycete fungus> Species 0.000 title claims abstract description 75
- 239000000049 pigment Substances 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000000855 fermentation Methods 0.000 claims abstract description 86
- 230000004151 fermentation Effects 0.000 claims abstract description 86
- -1 polyoxyethylene lauryl ether Polymers 0.000 claims abstract description 27
- 229920000259 polyoxyethylene lauryl ether Polymers 0.000 claims abstract description 27
- 239000001963 growth medium Substances 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 239000002609 medium Substances 0.000 claims description 35
- 150000003751 zinc Chemical class 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 6
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 6
- 229960001763 zinc sulfate Drugs 0.000 claims description 5
- 230000003698 anagen phase Effects 0.000 claims description 4
- 239000011592 zinc chloride Substances 0.000 claims description 3
- 235000005074 zinc chloride Nutrition 0.000 claims description 3
- 239000000693 micelle Substances 0.000 abstract description 9
- 239000001052 yellow pigment Substances 0.000 abstract description 7
- 238000000605 extraction Methods 0.000 abstract description 6
- 239000001054 red pigment Substances 0.000 abstract description 6
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 239000001053 orange pigment Substances 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000005063 solubilization Methods 0.000 abstract description 3
- 230000007928 solubilization Effects 0.000 abstract description 3
- 230000009471 action Effects 0.000 abstract description 2
- 239000007864 aqueous solution Substances 0.000 abstract description 2
- 244000113306 Monascus purpureus Species 0.000 description 20
- 235000002322 Monascus purpureus Nutrition 0.000 description 20
- 229940057059 monascus purpureus Drugs 0.000 description 20
- 239000012071 phase Substances 0.000 description 16
- 239000000243 solution Substances 0.000 description 16
- 239000002736 nonionic surfactant Substances 0.000 description 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- 238000011218 seed culture Methods 0.000 description 10
- 230000004913 activation Effects 0.000 description 8
- SFNALCNOMXIBKG-UHFFFAOYSA-N ethylene glycol monododecyl ether Chemical compound CCCCCCCCCCCCOCCO SFNALCNOMXIBKG-UHFFFAOYSA-N 0.000 description 7
- 239000001965 potato dextrose agar Substances 0.000 description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- 239000007836 KH2PO4 Substances 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical group CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 description 4
- 230000003213 activating effect Effects 0.000 description 4
- 230000002776 aggregation Effects 0.000 description 4
- 238000004220 aggregation Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 4
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- 244000005700 microbiome Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
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- 238000004519 manufacturing process Methods 0.000 description 2
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- 235000015097 nutrients Nutrition 0.000 description 2
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- 239000000126 substance Substances 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical class [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
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- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
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- 238000011081 inoculation Methods 0.000 description 1
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- 239000000203 mixture Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229940051841 polyoxyethylene ether Drugs 0.000 description 1
- 229920000056 polyoxyethylene ether Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
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- 239000006228 supernatant Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the technical field of biological fermentation, and provides a method for improving the color tone of monascus pigment, which comprises the following steps: mixing monascus seed liquid in logarithmic phase, polyoxyethylene lauryl ether and a fermentation culture medium, and performing fermentation culture to obtain the monascus pigment. The cloud point temperature of the polyoxyethylene lauryl ether selected by the invention is close to the fermentation temperature, so that the formation of a cloud point system of a fermentation system is promoted; meanwhile, the micelle has stronger hydrophilicity and shows strong beneficial solubilization, thereby further promoting the stability of micelle molecules in an aqueous solution; finally, under the combined action of the above principles, the extraction capability of the monascus pigment is improved, so that the color tone of the monascus pigment is improved. The experimental result shows that the extracellular color values (U/mL) of the red, orange and yellow pigments in the monascus pigment are 126.1, 103.5 and 99.6 respectively, and the color tone is 0.79 by utilizing the technical scheme provided by the invention.
Description
Technical Field
The invention relates to the technical field of biological fermentation, in particular to a method for improving the color tone of monascus pigment.
Background
Monascus pigment is a high-quality natural edible pigment prepared by fermenting filamentous fungi of monascus, and the demand of the monascus pigment in the field of food additives is increasing. At present, the production method of monascus pigment is mainly a liquid fermentation method, but in the traditional fermentation process, negative feedback inhibition caused by high concentration of products in thallus cells causes low pigment yield and low pigment tone, and after fermentation is finished, the pigments in the cells are separated and purified, so that a large amount of organic solvent is consumed.
The double-liquid-phase coupled extraction fermentation, also called milking fermentation, is an effective method for solving the problems at present, and specifically, a non-ionic surfactant is added into a fermentation medium, a fermentation system automatically forms an aggregation phase rich in non-ionic surfactant micelles and a dilute phase little in the micelles along with the formation of a cloud point system, and hydrophobic monascus pigment is extracted from the inside of cells to the outside of the cells and is greatly enriched in the aggregation phase in the fermentation process, so that the feedback inhibition of intracellular products is relieved, and the pigment yield is increased; in addition, intracellular pigments are greatly secreted to the outside of cells, and the use amount of organic solvents is reduced.
In the prior art, when the double liquid phase coupling extraction fermentation is utilized, the commonly adopted extracting agent is Span80, although the yield and the color tone of the monascus pigment can be improved compared with the traditional fermentation process, the color tone of the monascus pigment is relatively low.
Disclosure of Invention
In view of the above, the present invention provides a method for improving the color tone of monascus pigment, comprising:
mixing monascus seed liquid in logarithmic phase, polyoxyethylene lauryl ether and a fermentation culture medium, and performing fermentation culture to obtain the monascus pigment.
Preferably, the volume ratio of the monascus seed solution to the fermentation medium is 6-12%.
Preferably, the volume ratio of the monascus seed solution to the fermentation medium is 9-11%.
Preferably, the volume ratio of the monascus seed solution to the fermentation medium is 10%.
Preferably, the ratio of the mass of the polyoxyethylene lauryl ether to the volume of the fermentation medium is 30-34 g/L.
Preferably, the ratio of the mass of the polyoxyethylene lauryl ether to the volume of the fermentation medium is 31-33 g/L.
Preferably, the ratio of the mass of the polyoxyethylene lauryl ether to the volume of the fermentation medium is 32 g/L.
Preferably, after the monascus seed liquid in logarithmic phase, the polyoxyethylene lauryl ether and the fermentation medium are mixed, adding the water-soluble zinc salt for fermentation culture to obtain the monascus pigment.
Preferably, the water soluble zinc salt comprises at least one of zinc nitrate, zinc chloride and zinc sulfate.
Preferably, the ratio of the amount of said water-soluble zinc salt to the volume of water in the fermentation medium is 3.4 × 10-3～1×10-2mol/L。
The invention provides a method for improving the color tone of monascus pigment, which comprises the following steps: mixing monascus seed liquid in logarithmic phase, polyoxyethylene lauryl ether and a fermentation culture medium, and performing fermentation culture to obtain the monascus pigment. The invention selects the polyoxyethylene lauryl ether as the extractant, and the principle is that the cloud point temperature (42 ℃) of the polyoxyethylene lauryl ether is closest to the fermentation temperature (30 ℃) and has higher HLB value (HLB is 16.9). The cloud point is determined by the cloud point system of the fermentation system, and the extraction fermentation process must be accompanied by the formation of the cloud point system. HLB characterizes the hydrophilic-lipophilic balance of nonionic surfactants, the higher the HLB (HLB >13), the more hydrophilic the nonionic surfactant. Nonionic surfactants show strong beneficial solubilization when the HLB value is between 15 and 18. Therefore, the higher the HLB value, the better the stability of the micelle molecules of the nonionic surfactant in the solution. Only when these two conditions are satisfied simultaneously, the nonionic surfactant can exhibit a strong extraction ability of the monascus pigment, thereby improving the color tone. The experimental result shows that by utilizing the technical scheme provided by the invention, the extracellular color values of the red, orange and yellow pigments in the monascus pigment are respectively 126.1U/mL, 103.5U/mL and 99.6U/mL, and the color tone is 0.79, and the method is superior to that under the same condition, when Span80 is used as an extracting agent, the extracellular color values of the red, orange and yellow pigments are respectively 87.4U/mL, 73.9U/mL and 63.3U/mL, and the color tone is 0.72.
Detailed Description
The invention provides a method for improving the color tone of monascus pigment, which comprises the following steps:
mixing monascus seed liquid in logarithmic phase, polyoxyethylene lauryl ether and a fermentation culture medium, and performing fermentation culture to obtain the monascus pigment.
In the invention, the monascus seed solution in logarithmic growth phase is preferably obtained by activating and seed culturing the monascus purpureus strain in sequence. In the invention, the monascus in the monascus seed solution in the logarithmic growth phase is proliferated constantly and rapidly in a geometric series, and the size, the shape, the dyeing property, the physiological activity and the like of the bacteria in the period are in a typical state.
The invention has no special specification on the type of the monascus purpureus strain, and can be used for producing monascus purpureus strains of monascus pigment. The source of the monascus purpureus strain is not specially specified, and the monascus purpureus strain can be obtained by adopting a conventional commercial monascus purpureus strain. In the present embodiment, the monascus purpureus strain is preferably monascus purpureus strain NJ1, and the monascus purpureus strain NJ1 is conventionally commercially available.
The activating medium used in the present invention is not particularly limited, and any activating medium known to those skilled in the art may be used. In the embodiment of the invention, the activated culture medium is preferably a potato dextrose agar culture medium, PDA culture medium for short. The composition of the PDA culture medium is not specially specified in the invention, and the PDA culture medium well known to those skilled in the art can be adopted. In the present invention, the activation medium provides nutrients required for activation of the strain.
The activation mode is not particularly specified in the invention, and the monascus purpureus strain is subjected to activation culture by an activation mode well known to a person skilled in the art. In the embodiment of the invention, the activation is preferably carried out by streaking the monascus purpureus NJ1 strain on a PDA plate culture medium, and activating the strain for 3 generations in a constant temperature incubator at 30 ℃ for 3 days each generation. The invention is activated, strains in a preservation state are put into an activation culture medium for culture, and pure and strong cultures are obtained by stage-by-stage amplification culture, namely the cultures with vigorous activity and enough inoculation quantity are obtained.
The seed culture medium for the seed culture is not particularly specified, and the seed culture medium of the monascus purpureus strain well known to those skilled in the art can be adopted. In the embodiment of the invention, the seed culture medium is preferably added with 30g of glucose, 15g of soybean meal, 70g of glycerol, 10g of peptone and MgSO 2 (MgSO)4·7H2O1g、KH2PO4 2g、NaNO32g of the total weight. In the present invention, the seed culture medium provides nutrients required for the scale-up culture of the monascus purpureus strain.
The culture method of the seed culture is not specially specified, and the monascus purpureus strain can be cultured by adopting a seed culture method well known to a person skilled in the art. In the embodiment of the invention, the seed culture is preferably to inoculate the activated monascus purpureus strain to the seed solution, and to culture the strain in a shaking table with the temperature of 30 ℃ and the rotating speed of 200r/min for 48 hours. The method realizes the gradual expansion culture of the monascus purpureus strains through seed culture, and further obtains the monascus purpureus which is constantly and rapidly proliferated in a geometric series. Monascus in logarithmic growth and seed medium, known as monascus seed solution in logarithmic growth phase.
The invention has no special regulation on the fermentation culture medium for the fermentation culture, and the monascus pigment fermentation culture medium can be obtained by fermenting the monascus purpureus strain well known to the technical personnel in the field. In the embodiment of the invention, the fermentation medium is preferably added with 20g of rice flour, 90g of glycerol, 10g of peptone and MgSO 2 per liter of water4·7H2O 1g、KH2PO4 2.5g、NaNO35g of the total weight. In the present invention, the fermentation medium provides raw materials required for fermentation of microorganisms.
In the invention, the volume ratio of the monascus seed liquid to the fermentation medium is preferably 6-12%, more preferably 9-11%, and most preferably 10%. The monascus color tone obtained by controlling the usage amount of the monascus seed liquid and the fermentation medium in the range is the best.
The source of the polyoxyethylene lauryl ether is not particularly specified in the present invention, and conventional commercial products well known to those skilled in the art can be used. In the invention, the ratio of the mass of the polyoxyethylene lauryl ether to the volume of the fermentation medium is preferably 30-34 g/L, more preferably 31-33 g/L, and most preferably 32 g/L. The invention controls the dosage of the polyoxyethylene lauryl ether within the range, and the obtained monascus pigment has the best yield and color. According to the invention, the nonionic surfactant lauryl alcohol polyoxyethylene ether is added into the fermentation medium, along with the formation of a cloud point system, the fermentation system automatically forms an aggregation phase enriched by nonionic surfactant micelles and a dilute phase with few micelles, and the hydrophobic monascus pigment is extracted from the inside of cells to the outside of the cells and is enriched in the aggregation phase in a large amount in the fermentation process, so that the feedback inhibition of intracellular products is relieved, and the pigment yield is increased.
The invention has no special regulation on the mixing mode of the monascus seed liquid, the polyoxyethylene lauryl ether and the fermentation medium in the logarithmic phase, and the monascus seed liquid, the polyoxyethylene lauryl ether and the fermentation medium are uniformly mixed by adopting the mixing mode well known by the technical personnel in the field.
The monascus pigment is obtained by directly carrying out fermentation culture after mixing the monascus seed liquid in logarithmic phase, polyoxyethylene lauryl ether and a fermentation culture medium. The culture method of the fermentation culture is not specially specified, and the monascus pigment serving as a target fermentation product is obtained by fermenting raw materials of a fermentation culture medium through microorganisms by using a monascus purpureus strain in a fermentation method well known to a person skilled in the art.
After the fermentation is finished, the invention preferably carries out centrifugation and filtration on the fermented solution system in sequence to obtain the monascus pigment.
The centrifugation method is not particularly specified in the present invention, and insoluble impurities in the solution can be removed by centrifugation methods well known to those skilled in the art.
The filtration mode is not specially specified in the invention, and insoluble substances in the centrifuged solution can be separated by adopting a filtration mode which is well known to a person skilled in the art.
The invention provides a method for improving the color tone of monascus pigment, which comprises the following steps: mixing monascus seed liquid in logarithmic phase, polyoxyethylene lauryl ether and a fermentation culture medium, and performing fermentation culture to obtain the monascus pigment. The monascus pigment obtained by the method has high color tone by selecting the polyoxyethylene lauryl ether as the extracting agent. The cloud point temperature of the polyoxyethylene lauryl ether selected by the invention is close to the fermentation temperature, so that the formation of a cloud point system of a fermentation system is promoted; meanwhile, the micelle has stronger hydrophilicity and shows strong beneficial solubilization, thereby further promoting the stability of micelle molecules in an aqueous solution; finally, under the combined action of the above principles, the extraction capability of the monascus pigment is improved, so that the color tone of the monascus pigment is improved.
The monascus pigment is obtained by preferably adding water-soluble zinc salt after the monascus seed liquid in logarithmic phase, the polyoxyethylene lauryl ether and the fermentation medium are mixed, and performing fermentation culture. The invention further improves the color tone of the monascus pigment by adding the water-soluble zinc salt into the fermentation medium. In the present invention, Zn in the water-soluble zinc salt2+Has promoting effect on pigment production of Monascus, has large effect degree, has different effects on different strains, and can reach more than 100% of partial strains. Zn2+Has optimal promoting effect.
In the present invention, the water-soluble zinc salt preferably includes at least one of zinc nitrate, zinc chloride and zinc sulfate, more preferably zinc sulfate. In the invention, the zinc sulfate not only can provide zinc ions, but also can provide sulfur required in the growth process of microorganisms.
In the present invention, the ratio of the amount of the zinc salt substance to the volume of water in the fermentation medium is preferably 3.4X 10-3～1×10-2mol/L, more preferably 5X 10-3～8×10-3mol/L. The invention controls the dosage of the zinc salt in the range, and the obtained monascus pigment has better color tone.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Color number (U/mL) is OD value multiplied by dilution factor (wherein OD value refers to OD value of monascus pigment diluted by certain factor measured by spectrophotometer under wavelength of 410nm, 465nm and 505nm respectively); wherein, the color value (U/mL) of the haematochrome is OD (505nm) multiplied by dilution; orange pigment color number (U/mL) ═ OD (465nm) × dilution factor; yellow pigment color number (U/mL) ═ OD (410nm) × dilution factor.
The hue is equal to the yellow pigment color value/the red pigment color value, the hue is more than 1, the yellow pigment is more than the red pigment, and conversely, the red pigment is more than the yellow pigment.
Example 1
Experimental strains: strain NJ1 (commercially available in general) producing monascorubin
Step (1) Strain activation
The monascus purpureus NJ1 strain preserved at minus 80 ℃ is streaked on a PDA plate culture medium, and the strain is activated for 3 generations in a constant temperature incubator at 30 ℃, and the strains are cultured for 3 days per generation to obtain the activated strain.
Step (2) seed culture
Seed medium composition (g/L): adding 30g of glucose, 15g of soybean meal, 70g of glycerol, 10g of peptone and MgSO (MgSO) into each liter of water4·7H2O1g、KH2PO4 2g、NaNO3 2g。
Inoculating the activated strain obtained in the step (1) into a seed solution (liquid loading amount is 50mL/250mL), and culturing the seed solution in a shaking table at 30 ℃ and 200r/min for 48h to obtain the monascus seed solution in the logarithmic phase.
Step (3) fermentation culture
Fermentation broth medium (g/L): adding rice powder 20g, glycerol 90g, peptone 10g, and MgSO 2 into water per liter4·7H2O 1g、KH2PO4 2.5g、NaNO3 5g。
Inoculating 5mL of the monascus seed solution in the logarithmic phase obtained in the step (2) into a 250mL triangular flask containing 50mL of fermentation medium, and simultaneously adding a nonionic surfactant, namely laureth alcohol Brij35 (32 g of laureth alcohol is added in each liter of fermentation medium); shaking at 150r/min and 25 deg.C for 10 days, centrifuging at 12000r/min to obtain extracellular pigment, i.e. Monascus pigment, in the supernatant.
Step (4) testing the color value,
diluting the monascus pigment obtained in the step (3) by 180 times, and measuring by using an ultraviolet spectrophotometer. And (3) measuring results: the extracellular yields of the three pigments of red, orange and yellow in the fermentation broth are respectively 118.3U/mL, 94.2U/mL and 89.5U/mL, the color tone of the monascus pigment is calculated to be 0.76, and red is more than yellow.
Example 2
The operation was the same as in example 1, except that 2g/L ZnSO was added to the fermentation broth at the time of preparing the fermentation broth4·7H2O (i.e. 6.96X 10 per liter of water-3mol of ZnSO4·7H2O). And (3) measuring results: the extracellular yields of the three pigments of red, orange and yellow in the fermentation liquor are respectively 126.1U/mL, 103.5U/mL and 99.6U/mL, the color tone is 0.79, and the red is more than the yellow.
Comparative example 1
The operation was the same as in example 1, except that no nonionic surfactant was added. And (3) measuring results: the extracellular yields of the three pigments of red, orange and yellow in the fermentation liquor are respectively 52.2U/mL, 43.7U/mL and 36.2U/mL, the color tone is 0.69, and the red is more than the yellow.
Comparative example 2:
the operation was the same as in example 1, except that the nonionic surfactant was added from polyoxyethylene lauryl ether Brij35 to Span 80. And (3) measuring results: the extracellular yields of the three pigments of red, orange and yellow in the fermentation liquor are respectively 87.4U/mL, 73.9U/mL and 63.3U/mL, the color tone is 0.72, and the red is more than the yellow.
As can be seen from example 1 and comparative examples 1 to 2: compared with the method without adding the nonionic surfactant, the method has the advantages that the tone is improved by 10.1 percent by adding the nonionic surfactant into the fermentation liquor; the addition of Brij35 increased the hue by 5.6% compared to Span 80.
As can be seen from example 2 and comparative examples 1 to 2: compared with the method without adding the non-ionic surfactant, the method simultaneously adds Brij35 into the fermentation liquor to improve the color tone by 14.5 percent, and compared with the method without adding the zinc salt into Brij35, the method improves the color tone by 3.9 percent.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A method for improving the color tone of monascus pigment comprises the following steps:
mixing monascus seed liquid in logarithmic phase, polyoxyethylene lauryl ether and a fermentation culture medium, and performing fermentation culture to obtain the monascus pigment.
2. The method according to claim 1, wherein the volume ratio of the monascus seed solution to the fermentation medium is 6-12%.
3. The method according to claim 2, wherein the volume ratio of the monascus seed solution to the fermentation medium is 9-11%.
4. The method according to claim 3, wherein the ratio of the monascus seed solution to the fermentation medium is 10% by volume.
5. The method according to claim 1, wherein the ratio of the mass of the polyoxyethylene lauryl ether to the volume of the fermentation medium is 30-34 g/L.
6. The method according to claim 5, wherein the ratio of the mass of the polyoxyethylene lauryl ether to the volume of the fermentation medium is 31-33 g/L.
7. The method according to claim 6, wherein the ratio of the mass of the polyoxyethylene lauryl ether to the volume of the fermentation medium is 32 g/L.
8. The method as claimed in claim 1, wherein the monascus pigment is obtained by adding water-soluble zinc salt after the monascus seed solution in logarithmic growth phase, the polyoxyethylene lauryl ether and the fermentation medium are mixed, and performing fermentation culture.
9. The method of claim 8, wherein the water soluble zinc salt comprises at least one of zinc nitrate, zinc chloride, and zinc sulfate.
10. The method according to claim 8, wherein the ratio of the amount of said water-soluble zinc salt to the volume of water in the fermentation medium is 3.4 x 10-3～1×10-2mol/L。
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| CN202210040184.9A Pending CN114350526A (en) | 2022-01-14 | 2022-01-14 | Method for improving monascus pigment tone |
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