CN104945188A - Compound microbial organic fertilizer for improvement of saline-alkali soil and preparation method thereof - Google Patents
Compound microbial organic fertilizer for improvement of saline-alkali soil and preparation method thereof Download PDFInfo
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Abstract
The invention provides a compound microbial organic fertilizer for improvement of saline-alkali soil and a preparation method thereof. The compound microbial organic fertilizer for improvement of saline-alkali soil is prepared from liquid waste generated by a sugar refinery through filtering, biological culture, separation, baking and other steps; in the preparation process, the used strain Stenotrophomonas maltophilia TY-C1 is collected at CGMCC (China General Microbiological Culture Collection Center) on March 17, 2015 and has a collection number of CGMCC No. 10636, wherein the address of CGMCC is Institute of Microbiology, Chinese Academy of Science, yard 1, number 3, Beichen west road, Chaoyang district, Beijing. The compound microbial organic fertilizer has the functions of neutralizing the alkaline environment of the saline-alkali soil and releasing elements such as N, P and K.
Description
Technical field
The invention belongs to changing waste into resources and utilize technical field, be specifically related to a kind of complex microorganism organic fertilizer being applicable to alkaline land improving and preparation method thereof.
Background technology
The salinization in soil is a worldwide ecological problem, and the saline Land degree in current global range presents obvious ascendant trend.China's saltings total area account for that the whole nation can utilize land area 4.88%, and to increase with the speed of annual 1%.The research and practice work in Saline-alkali Field Control and improvement is all being carried out in countries in the world always energetically, although a part of saltings obtains effective utilization, aspect is utilized all not obtain key breakthrough progress to the control of Pedotransfer function and alkaline land improving.Microbial fertilizer improves soil physical property by the vital movement of microorganism, increase the supply of plant nutrient in soil, harmful microbe can be suppressed movable simultaneously, improve the micro-ecological environment of soil, thus raising crop yield, there is good application prospect, but also lack related microorganisms fertilizer and preparation method thereof at present.
Summary of the invention
The invention provides a kind of complex microorganism organic fertilizer being applicable to alkaline land improving and preparation method thereof.The concrete steps of described preparation method are as follows:
(1) be the filter cloth of 5mm by 8000mL sugar refinery waste liquid by aperture, be then aeration 2 hours under the condition of 150mL/min at gas flow, obtain mixed solution X;
(2) in mixed solution X, 8mL trace metal liquid is added and 100mL massfraction is the lactic acid solution of 25%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 6 hours under the condition of 150mL/min at gas flow, obtain mixed solution Y, wherein the consisting of of trace metal liquid: CoCl
26H
2o:33mgL
-1, CuCl
2: 0.26mgL
-1, H
3bO
3: 4.0mgL
-1, MnCl
24H
2o:22mgL
-1, Na
2moO
42H
2o:2.0mgL
-1, NiCl
2.2H
2o:3.0mgL
-1, ZnCl
2: 2.7mgL
-1; The bacteria containing amount of germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is 5 × 10
10cFU/ml, consisting of of nutrient solution: maltose 5gL
-1, NH
4cl:1.9gL
-1, KH
2pO
4: 1.9gL
-1, MgCl
2: 0.27gL
-1, CaCl
22H
2o:0.13gL
-1;
(3) in mixed solution Y, 48mL nutritive salt substratum is added and 120mL massfraction is the lactic acid solution of 35%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 6 hours under the condition of 150mL/min at gas flow, obtain mixed solution Z, wherein the consisting of of nutritive salt substratum: NH
4nO
3: 1.7gL
-1, K
2hPO
4: 1.4gL
-1, MgCl
2: 0.22gL
-1, CaCl
22H
2o:0.16gL
-1;
(4) be the NH of 0.25mol/L by 100mL concentration
4cl solution and 100mL concentration are the CaCl of 0.15mol/L
2solution, obtains mixed liquor A after mixing;
(5) be (NH of 0.15mol/L by 100mL concentration
4)
3pO
4solution is slowly added drop-wise in 200mL mixed liquor A under the condition stirred, and obtains mixed liquid B;
(6) in mixed liquid B, dropwise add the vitriol oil to clarification, obtain mixed solution C;
(7) regulate the pH value of mixed solution C to be 3.5 with the hydrochloric acid soln of concentration to be the NaOH solution of 0.02mol/L and concentration be 0.02mol/L, obtain mixed solution D;
(8) in mixed solution D, add the aqueous solution of urea that 100mL concentration is 1.4mol/L, after mixing, obtain mixed solution E;
(9) in mixed solution E, add the calcio-disodium edetate solution that 50mL concentration is 0.25mol/L, after mixing, obtain mixed solution F;
(10) by 60mL massfraction be 35% aqueous solution of urea be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution G in 35 minutes; By 10mL massfraction be 25% formalin be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution H in 35 minutes;
(11) in mixed solution G, drip the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L under 1000r/min agitation condition, the pH value making solution is 7.2 ~ 7.4, obtains mixed liquor I;
(12) mixed solution H is joined in mixed liquor I under 1000r/min agitation condition, be then placed in 43 DEG C of water-baths and stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution J;
(13) in mixed solution J, under 1000r/min agitation condition, drip the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L, the pH value of solution is made to be 3.7 ~ 3.9, then be placed in 38 DEG C of water-baths to stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution K;
(14) 1600mL deionized water is added drop-wise in mixed solution K under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution L;
(15) be the KNO of 0.25mol/L by 100mL concentration
3solution and 100mL concentration are the Ca (N0 of 1.55mol/L
3)
2solution fully mixes, and is then placed in 43 DEG C of water-bath constant temperature 35 minutes, obtains mixed solution M;
(16) 60mL mixed solution L is added drop-wise in mixed solution M under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution N;
(17) 60mL mixed solution F is added drop-wise in mixed solution N under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution O;
(18) by 100mL massfraction be 5.5% sodium alginate soln be heated to 75 DEG C, then 100mL mixed solution O is added, 8min is stirred under 1000r/min condition, in 25 DEG C of water-baths, constant temperature crosses aperture after 15 hours is that solid particulate isolated by the sieve of 5mm, and then with deionized water wash 3 isolated solid particulates, lyophilize obtains solid particulate A;
(19) solid particulate A is cultivated 36h in 250mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution, then take out and air-dryly obtain solid particulate B;
(20) solid particulate B is joined in 2000mL mixed solution Z, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid P;
(21) 2000mL mixed solution Z is joined in suspension liquid P under 1000r/min agitation condition, obtain mixed solution Q;
(22) in mixed solution Q, 25mL mixed solution L is added and 100mL massfraction is the lactic acid solution of 35%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R;
(23) in suspension liquid Q, 65mL nutritive salt substratum is added, stir under 1000r/min condition after 5 minutes and add 25mL mixed solution F, and then add 2000mL mixed solution Z and 110mL massfraction is the lactic acid solution of 15%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R;
(24) in suspension liquid R, add 4mL trace metal liquid, under 1000r/min condition, stir 15min, and then add 55mL nutritive salt substratum, obtain mixed solution S;
(25) in mixed solution S, 2000mL mixed solution Z is added and 90mL massfraction is the lactic acid solution of 20%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid T;
(26) suspension liquid T is dried under 45 DEG C of conditions, the complex microorganism organic fertilizer for alkaline land improving can be obtained.
Bacterial strain germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 used, on March 17th, 2015 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, this is centrally located at No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, and culture presevation number is CGMCC No.10636.
Described bacterial strain germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 is separated by the following method and obtains:
(1) choose wastewater from sugar plant pond bed mud 1000g to be positioned in 4 DEG C of refrigerators and to save backup;
(2) in open glass bottle, adding 500 ~ 600mL basic medium, is the bed mud 250g added after stirring 40 ~ 50min under the condition of 100r/min in step (1) at rotating speed; Then to be placed in temperature be the environment of 25 ~ 30 DEG C is stir acclimating under the condition of 100r/min to cultivate 10d at rotating speed, and the regular basic medium that adds in vial remains unchanged with the water and soil ratio maintaining mud system in vial in the training period; Wherein the consisting of of basic medium: NH
4cl:1.9gL
-1, KH
2pO
4: 1.9gL
-1, MgCl
2: 0.27gL
-1, CaCl
22H
2o:0.13gL
-1;
(4) to volume be 100 ~ 200mL Erlenmeyer flask in add the maltose solution that 20mL concentration is 5g/L;
(5) in the Erlenmeyer flask in step (4), 50mL basic medium, 0.5mL trace metal liquid and 0.1mL vitamin c solution is entered; Wherein the consisting of of trace metal liquid: CoCl
26H
2o:33mgL
-1, CuCl
2: 0.26mgL
-1, H
3bO
3: 4.0mgL
-1, MnCl
24H
2o:22mgL
-1, Na
2moO
42H
2o:2.0mgL
-1, NiCl
22H
2o:3.0mgL
-1, ZnCl
2: 2.7mgL
-1; .
(6) the bed mud 0.35g that access step (3) is tamed in the Erlenmeyer flask in step (5), then to be placed in temperature be 25 ~ 30 DEG C of rotating speeds is that the vibrator of 100r/min is cultivated 2 ~ 3 days, opens bottleneck every day once to recover the dissolved oxygen in Erlenmeyer flask; The ratio being 200 ~ 250: 1 in inoculation volume ratio proceeds to another by the Erlenmeyer flask after step (4) to (5) process, can obtain flora solution after switching number of times is greater than 4;
(7) to volume be 500mL Erlenmeyer flask in add 400mL basic medium, vitamin c solution that 1.0mL trace metal liquid, 0.1mL mass concentration are 1g/L, shake up rear for subsequent use as liquid nutrient medium;
(8) to volume be 150mL Erlenmeyer flask in add the liquid nutrient medium and 0.60g agar that 50mL prepares by step (7), then be sterilizing 20 minutes in the pressure kettle of 121 DEG C in temperature, when being at room temperature cooled to 65 ~ 70 DEG C, in Bechtop, be poured into volume is in the culture dish of 160mL, and this culture dish is placed 2 days by the moisture unnecessary for removing media surface in Bechtop;
(9) in Bechtop, add the maltose solution that 0.5mL concentration is 5g/L in the culture dish in step (8), tilt rotation culture dish makes maltose solution be uniformly distributed back and forth;
(10) to volume be 25mL Erlenmeyer flask in add the liquid nutrient medium and 0.25g agar that 10mL prepares by step (7), be sterilizing 20 minutes in the pressure kettle of 121 DEG C in temperature, in Bechtop, be cooled to 38 ~ 40 DEG C;
(11) in Bechtop, in the Erlenmeyer flask of step (10), add the flora solution that 1mL step (6) obtains, shaking up rear absorption 1mL slowly adds in the culture dish in step (9), and tilt rotation culture dish makes inoculation have the culture medium solution of mixed bacterial to be uniformly distributed back and forth; Culture dish is put into the incubator cultivation observation that temperature is 25 DEG C, just can find that there is obvious bacterium colony after 10 ~ 15 days and occur;
(12) bacterium colony in Bechtop in picking step (11) culture dish, carries out repeated isolation, and separation and purification more than 3 cycles can obtain pure strain.
The invention has the beneficial effects as follows, with the alkaline environment in saltings during obtained complex microorganism organic fertilizer has, the functions such as nitrogen, phosphorus, potassium can be discharged, its organic composition and humic acids effectively can cushion soil fluctuation to soil property generation under extraneous disturbed condition, can be used in improvement soil, stimulate plant growth, improve agricultural product quality.
Embodiment
The present invention is further illustrated below in conjunction with example.
Be the filter cloth of 5mm by 8000mL sugar refinery waste liquid by aperture, be then aeration 2 hours under the condition of 150mL/min at gas flow, obtain mixed solution X; 8mL trace metal liquid is added and 100mL massfraction is the lactic acid solution of 25% in mixed solution X, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 6 hours under the condition of 150mL/min at gas flow, obtain mixed solution Y, wherein the consisting of of trace metal liquid: CoCl
26H
2o:33mgL
-1, CuCl
2: 0.26mgL
-1, H
3bO
3: 4.0mgL
-1, MnCl
24H
2o:22mgL
-1, Na
2moO
42H
2o:2.0mgL
-1, NiCl
22H
2o:3.0mgL
-1, ZnCl
2: 2.7mgL
-1; The bacteria containing amount of germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is 5 × 10
10cFU/ml, consisting of of nutrient solution: maltose 5gL
-1, NH
4cl:1.9gL
-1, KH
2pO
4: 1.9gL
-1, MgCl
2: 0.27gL
-1, CaCl
22H
2o:0.13gL
-1; 48mL nutritive salt substratum is added and 120mL massfraction is the lactic acid solution of 35% in mixed solution Y, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 6 hours under the condition of 150mL/min at gas flow, obtain mixed solution Z, wherein the consisting of of nutritive salt substratum: NH
4nO
3: 1.7gL
-1, K
2hPO
4: 1.4gL
-1, MgCl
2: 0.22gL
-1, CaCl
22H
2o:0.16gL
-1;
Be the NH of 0.25mol/L by 100mL concentration
4cl solution and 100mL concentration are the CaCl of 0.15mol/L
2solution, obtains mixed liquor A after mixing; Be (the NH of 0.15mol/L by 100mL concentration
4)
3pO
4solution is slowly added drop-wise in 200mL mixed liquor A under the condition stirred, and obtains mixed liquid B; In mixed liquid B, dropwise add the vitriol oil to clarification, obtain mixed solution C; Regulate the pH value of mixed solution C to be 3.5 with the hydrochloric acid soln of concentration to be the NaOH solution of 0.02mol/L and concentration be 0.02mol/L, obtain mixed solution D; In mixed solution D, add the aqueous solution of urea that 100mL concentration is 1.4mol/L, after mixing, obtain mixed solution E; In mixed solution E, add the calcio-disodium edetate solution that 50mL concentration is 0.25mol/L, after mixing, obtain mixed solution F;
By 60mL massfraction be 35% aqueous solution of urea be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution G in 35 minutes; By 10mL massfraction be 25% formalin be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution H in 35 minutes; In mixed solution G, drip the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L under 1000r/min agitation condition, the pH value making solution is 7.2 ~ 7.4, obtains mixed liquor I;
Mixed solution H is joined in mixed liquor I under 1000r/min agitation condition, is then placed in 43 DEG C of water-baths and stirs 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution J; Under 1000r/min agitation condition, the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L is dripped in mixed solution J, the pH value of solution is made to be 3.7 ~ 3.9, then be placed in 38 DEG C of water-baths to stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution K; 1600mL deionized water is added drop-wise in mixed solution K under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution L;
Be the KNO of 0.25mol/L by 100mL concentration
3solution and 100mL concentration are the Ca (NO of 1.55mol/L
3)
2solution fully mixes, and is then placed in 43 DEG C of water-bath constant temperature 35 minutes, obtains mixed solution M; 60mL mixed solution L is added drop-wise in mixed solution M under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution N;
60mL mixed solution F is added drop-wise in mixed solution N under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution O; By 100mL massfraction be 5.5% sodium alginate soln be heated to 75 DEG C, then 100mL mixed solution O is added, 8min is stirred under 1000r/min condition, in 25 DEG C of water-baths, constant temperature crosses aperture after 15 hours is that solid particulate isolated by the sieve of 5mm, and then with deionized water wash 3 isolated solid particulates, lyophilize obtains solid particulate A; Solid particulate A is cultivated 36h in 250mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution, then takes out and air-dryly obtain solid particulate B; Solid particulate B is joined in 2000mL mixed solution Z, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid P; 2000mL mixed solution Z is joined in suspension liquid P under 1000r/min agitation condition, obtains mixed solution Q;
25mL mixed solution L is added and 100mL massfraction is the lactic acid solution of 35% in mixed solution Q, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R;
65mL nutritive salt substratum is added in suspension liquid Q, stir under 1000r/min condition after 5 minutes and add 25mL mixed solution F, and then add 2000mL mixed solution Z and 110mL massfraction is the lactic acid solution of 15%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R;
In suspension liquid R, add 4mL trace metal liquid, under 1000r/min condition, stir 15min, and then add 55mL nutritive salt substratum, obtain mixed solution S;
2000mL mixed solution Z is added and 90mL massfraction is the lactic acid solution of 20% in mixed solution S, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid T; Suspension liquid T is dried under 45 DEG C of conditions, the complex microorganism organic fertilizer for alkaline land improving can be obtained.
The alkaline environment in during the complex microorganism organic fertilizer obtained has and saltings, the functions such as nitrogen, phosphorus, potassium can be discharged, its organic composition and humic acids effectively can cushion soil fluctuation to soil property generation under extraneous disturbed condition, can be used in improvement soil, stimulate plant growth, improve agricultural product quality.
Claims (2)
1. a complex microorganism organic fertilizer, this complex microorganism organic fertilizer is applicable to alkaline land improving, it is characterized in that, the preparation method of described complex microorganism organic fertilizer is as follows:
(1) be the filter cloth of 5mm by 8000mL sugar refinery waste liquid by aperture, be then aeration 2 hours under the condition of 150mL/min at gas flow, obtain mixed solution X;
(2) in mixed solution X, 8mL trace metal liquid is added and 100mL massfraction is the lactic acid solution of 25%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 6 hours under the condition of 150mL/min at gas flow, obtain mixed solution Y, wherein the consisting of of trace metal liquid: CoCl
26H
2o:33mgL
-1, CuCl
2: 0.26mgL
-1, H
3bO
3: 4.0mgL
-1, MnCl
24H
2o:22mgL
-1, Na
2moO
42H
2o:2.0mgL
-1, NiCl
22H
2o:3.0mgL
-1, ZnCl
2: 2.7mgL
-1; The bacteria containing amount of germ oligotrophy unit cell Stenotrophomonasmaltophilia TY-C1 nutrient solution is 5 × 10
10cFU/ml, consisting of of nutrient solution: maltose 5gL
-1, NH
4cl:1.9gL
-1, KH
2pO
4: 1.9gL
-1, MgCl
2: 0.27gL
-1, CaCl
22H
2o:0.13gL
-1;
(3) in mixed solution Y, 48mL nutritive salt substratum is added and 120mL massfraction is the lactic acid solution of 35%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 6 hours under the condition of 150mL/min at gas flow, obtain mixed solution Z, wherein the consisting of of nutritive salt substratum: NH
4nO
3: 1.7gL
-1, K
2hPO
4: 1.4gL
-1, MgCl
2: 0.22gL
-1, CaCl
22H
2o:0.16gL
-1;
(4) be the NH of 0.25mol/L by 100mL concentration
4cl solution and 100mL concentration are the CaCl of 0.15mol/L
2solution, obtains mixed liquor A after mixing;
(5) be (NH of 0.15mol/L by 100mL concentration
4)
3pO
4solution is slowly added drop-wise in 200mL mixed liquor A under the condition stirred, and obtains mixed liquid B;
(6) in mixed liquid B, dropwise add the vitriol oil to clarification, obtain mixed solution C;
(7) regulate the pH value of mixed solution C to be 3.5 with the hydrochloric acid soln of concentration to be the NaOH solution of 0.02mol/L and concentration be 0.02mol/L, obtain mixed solution D;
(8) in mixed solution D, add the aqueous solution of urea that 100mL concentration is 1.4mol/L, after mixing, obtain mixed solution E;
(9) in mixed solution E, the calcio-disodium edetate solution that 50mL concentration is 0.25mol/L is added, must to mixed solution F after mixing;
(10) by 60mL massfraction be 35% aqueous solution of urea be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution G in 35 minutes; By 10mL massfraction be 25% formalin be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution H in 35 minutes;
(11) in mixed solution G, drip the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L under 1000r/min agitation condition, the pH value making solution is 7.2 ~ 7.4, obtains mixed liquor I;
(12) mixed solution H is joined in mixed liquor I under 1000r/min agitation condition, be then placed in 43 DEG C of water-baths and stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution J;
(13) in mixed solution J, under 1000r/min agitation condition, drip the HCl solution that the NaOH solution that concentration is 0.0lmol/L and concentration are 0.005mol/L, the pH value of solution is made to be 3.7 ~ 3.9, then be placed in 38 DEG C of water-baths to stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution K;
(14) 1600mL deionized water is added drop-wise in mixed solution K under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution L;
(15) be the KNO of 0.25mol/L by 100mL concentration
3solution and 100mL concentration are the Ca (NO of 1.55mol/L
3)
2solution fully mixes, and is then placed in 43 DEG C of water-bath constant temperature 35 minutes, obtains mixed solution M;
(16) 60mL mixed solution L is added drop-wise in mixed solution M under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution N;
(17) 60mL mixed solution F is added drop-wise in mixed solution N under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution O;
(18) by 100mL massfraction be 5.5% sodium alginate soln be heated to 75 DEG C, then 100mL mixed solution O is added, 8min is stirred under 1000r/min condition, in 25 DEG C of water-baths, constant temperature crosses aperture after 15 hours is that solid particulate isolated by the sieve of 5mm, and then with deionized water wash 3 isolated solid particulates, lyophilize obtains solid particulate A;
(19) solid particulate A is cultivated 36h in 250mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution, then take out and air-dryly obtain solid particulate B;
(20) solid particulate B is joined in 2000mL mixed solution Z, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid P;
(21) 2000mL mixed solution Z is joined in suspension liquid P under 1000r/min agitation condition, obtain mixed solution Q;
(22) in mixed solution Q, 25mL mixed solution L is added and 100mL massfraction is the lactic acid solution of 35%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R;
(23) in suspension liquid Q, 65mL nutritive salt substratum is added, stir under 1000r/min condition after 5 minutes and add 25mL mixed solution F, and then add 2000mL mixed solution Z and 110mL massfraction is the lactic acid solution of 15%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R;
(24) in suspension liquid R, add 4mL trace metal liquid, under 1000r/min condition, stir 15min, and then add 55mL nutritive salt substratum, obtain mixed solution S;
(25) in mixed solution S, 2000mL mixed solution Z is added and 90mL massfraction is the lactic acid solution of 20%, 15min is stirred under 1000r/min condition, then 50mL germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is added, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid T;
(26) suspension liquid T is dried under 45 DEG C of conditions, the complex microorganism organic fertilizer for alkaline land improving can be obtained.
2. complex microorganism organic fertilizer according to claim 1, it is characterized in that, the bacterial strain germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 applied in the preparation method of described complex microorganism organic fertilizer, on March 17th, 2015 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, this is centrally located at No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, and culture presevation number is CGMCC No.10636.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111362737A (en) * | 2018-12-25 | 2020-07-03 | 四川绿高生态农业科技开发有限公司 | Method for producing microbial organic fertilizer by taking cultivation waste as raw material |
| CN111434644A (en) * | 2018-12-25 | 2020-07-21 | 四川绿高生态农业科技开发有限公司 | Preparation method of composite microbial organic liquid fertilizer suitable for soil heavy metal remediation |
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| CN102381822A (en) * | 2011-07-18 | 2012-03-21 | 刘建伦 | Multi-strain compound microbial high-temperature rapid-digestion sludge harmless treatment method |
| CN102936166A (en) * | 2012-11-29 | 2013-02-20 | 南宁市英德肥业有限责任公司 | Water-soluble organic/inorganic compound fertilizer prepared by molasses fermentation liquor and preparation method of fertilizer |
| CN103074277A (en) * | 2012-12-28 | 2013-05-01 | 浙江至美环境科技有限公司 | Denitrifying bacterium and application thereof |
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| CN101555171A (en) * | 2009-05-14 | 2009-10-14 | 许绪满 | Method for producing micro organic matters by marine culture sludge and sugar refinery filtration sludge |
| CN102381822A (en) * | 2011-07-18 | 2012-03-21 | 刘建伦 | Multi-strain compound microbial high-temperature rapid-digestion sludge harmless treatment method |
| CN102936166A (en) * | 2012-11-29 | 2013-02-20 | 南宁市英德肥业有限责任公司 | Water-soluble organic/inorganic compound fertilizer prepared by molasses fermentation liquor and preparation method of fertilizer |
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| CN111362737A (en) * | 2018-12-25 | 2020-07-03 | 四川绿高生态农业科技开发有限公司 | Method for producing microbial organic fertilizer by taking cultivation waste as raw material |
| CN111434644A (en) * | 2018-12-25 | 2020-07-21 | 四川绿高生态农业科技开发有限公司 | Preparation method of composite microbial organic liquid fertilizer suitable for soil heavy metal remediation |
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