CN104945081A - Process for producing microbial organic fertilizer by taking waste liquid from sugar refinery as raw material - Google Patents

Process for producing microbial organic fertilizer by taking waste liquid from sugar refinery as raw material Download PDF

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CN104945081A
CN104945081A CN201510172463.0A CN201510172463A CN104945081A CN 104945081 A CN104945081 A CN 104945081A CN 201510172463 A CN201510172463 A CN 201510172463A CN 104945081 A CN104945081 A CN 104945081A
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曹勇
袁静
许新宜
吴轶俊
丁爱中
张晖
王金生
豆俊峰
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Qing Yu Environmental Protection Technology Co Ltd Of Zhuhai City
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Qing Yu Environmental Protection Technology Co Ltd Of Zhuhai City
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention provides a process for producing a microbial organic fertilizer by taking waste liquid from a sugar refinery as a raw material. The process comprises the steps of aerating after filtering the waste liquid from the sugar refinery; then, adding molten trace metal, a nutritive salt culture medium and a Stenotrophomonas maltophilia TY-C1 culture solution; and after culturing, separating, drying and the like to obtain the microbial organic fertilizer. The prepared microbial organic fertilizer has the function of releasing nitrogen, phosphorus, potassium and the like and can be used for improving soil, stimulating crop growth and improving the quality of an agricultural product.

Description

With the production technique of sugar refinery waste liquid for raw material production microbial organic fertilizer
Technical field
The invention belongs to changing waste into resources and utilize technical field, be specifically related to a kind of with the production technique of sugar refinery waste liquid for raw material production microbial organic fertilizer.
Background technology
Along with the development that sugaring industry is advanced by leaps and bounds, the problem of environmental pollution thereupon produced also progressively displays.Sugar refinery waste liquid is generally containing organism and sugar, COD and BOD is very high, and colourity is high, and directly enter water body and can cause potential harm to the growth of hydrocoles and population health, direct irrigation and drainage farmland not only burns crops to death, and makes soil compaction.Containing a large amount of organic substance in the waste liquid of sugar refinery, the element such as nitrogenous, phosphorus, potassium is higher simultaneously, and be substantially free of Toxic matter, thus biodegradability is good.If be used to the valuable components in the waste liquid of sugar refinery, carried out Resource recovery, both eliminated pollution, create value again, and be conducive to the Sustainable development of related industries, development potentiality is huge.Wherein, with sugar refinery waste liquid for raw material production fertilizer is a kind of industry having very much dynamogenetic value, but also lack relevant production technique at present.
Summary of the invention
The invention provides a kind of with the production technique of sugar refinery waste liquid for raw material production microbial organic fertilizer.The concrete steps of described production technique are as follows:
(1) be the filter cloth of 5mm by 8000mL sugar refinery waste liquid by aperture, be then aeration 2 hours under the condition of 150mL/min at gas flow, obtain mixed solution X;
(2) in mixed solution X, add 8mL trace metal liquid, under 1000r/min condition, stir 15min, obtain mixed solution Y, wherein the consisting of of trace metal liquid: CoCl 26H 2o:33mgL -1, CuCl 2: 0.26mgL -1, H 3bO 3: 4.0mgL -1, MnCl 24H 2o:22mgL -1, Na 2moO 42H 2o:2.0mgL -1, NiCl 22H 2o:3.0mgL -1, ZnCl 2: 2.7mgL -1;
(3) in mixed solution Y, add 48mL nutritive salt substratum, under 1000r/min condition, stir 15min, obtain mixed solution Z, wherein the consisting of of nutritive salt substratum: NH 4nO 3: 1.7gL -1, K 2hPO 4: 1.4gL -1, MgCl 2: 0.22gL -1, CaCl 22H 2o:0.16gL -1;
(4) be the NH of 0.25mol/L by 100mL concentration 4cl solution and 100mL concentration are the CaCl of 0.15mol/L 2solution, obtains mixed liquor A after mixing;
(5) be (NH of 0.15mol/L by 100mL concentration 4) 3pO 4solution is slowly added drop-wise in 200mL mixed liquor A under the condition stirred, and obtains mixed liquid B;
(6) in mixed liquid B, dropwise add the vitriol oil to clarification, obtain mixed solution C;
(7) regulate the pH value of mixed solution C to be 3.5 with the hydrochloric acid soln of concentration to be the NaOH solution of 0.02mol/L and concentration be 0.02mol/L, obtain mixed solution D;
(8) in mixed solution D, add the aqueous solution of urea that 100mL concentration is 1.4mol/L, after mixing, obtain mixed solution E;
(9) in mixed solution E, add the calcio-disodium edetate solution that 50mL concentration is 0.25mol/L, after mixing, obtain mixed solution F;
(10) by 60mL massfraction be 35% aqueous solution of urea be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution G in 35 minutes; By 10mL massfraction be 25% formalin be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution H in 35 minutes;
(11) in mixed solution G, drip the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L under 1000r/min agitation condition, the pH value making solution is 7.2 ~ 7.4, obtains mixed liquor I;
(12) mixed solution H is joined in mixed liquor I under 1000r/min agitation condition, be then placed in 43 DEG C of water-baths and stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution J;
(13) in mixed solution J, under 1000r/min agitation condition, drip the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L, the pH value of solution is made to be 3.7 ~ 3.9, then be placed in 38 DEG C of water-baths to stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution K;
(14) 1600mL deionized water is added drop-wise in mixed solution K under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution L;
(15) be the KNO of 0.25mol/L by 100mL concentration 3solution and 100mL concentration are the Ca (NO of 1.55mol/L 3) 2solution fully mixes, and is then placed in 43 DEG C of water-bath constant temperature 35 minutes, obtains mixed solution M;
(16) 60mL mixed solution L is added drop-wise in mixed solution M under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution N;
(17) 60mL mixed solution F is added drop-wise in mixed solution N under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution O;
(18) by 100mL massfraction be 5.5% sodium alginate soln be heated to 75 DEG C, then 100mL mixed solution O is added, 8min is stirred under 1000r/min condition, in 25 DEG C of water-baths, constant temperature crosses aperture after 15 hours is that solid particulate isolated by the sieve of 5mm, and then with deionized water wash 3 isolated solid particulates, lyophilize obtains solid particulate A;
(19) solid particulate A is cultivated 36h in germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution, then take out and air-dryly obtain solid particulate B, wherein the bacteria containing amount of germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is 5 × 10 10cFU/ml, consisting of of nutrient solution: maltose 5gL -1, NH 4cl:1.9gL -1, KH 2pO 4: 1.9gL -1, MgCl 2: 0.27gL -1, CaCl 22H 2o:0.13gL -1;
(20) solid particulate B is joined in 2000mL mixed solution Z, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid P;
(21) 2000mL mixed solution Z is joined in suspension liquid P under 1000r/min agitation condition, obtain mixed solution Q;
(22) in mixed solution Q, add 25mL mixed solution L, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R;
(23) in suspension liquid Q, 65mL nutritive salt substratum is added, stir under 1000r/min condition after 5 minutes and add 25mL mixed solution F, and then add 2000mL mixed solution Z, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R; Wherein the consisting of of nutritive salt substratum: NH 4nO 3: 1.7gL -1, K 2hPO 4: 1.4gL -1, MgCl 2: 0.22gL -1, CaCl 22H 2o:0.16gL -1;
(24) in suspension liquid R, add 4mL trace metal liquid, under 1000r/min condition, stir 15min, and then add 55mL nutritive salt substratum, obtain mixed solution S, wherein the consisting of of trace metal liquid: CoCl 26H 2o:33mgL -1, CuCl 2: 0.26mgL -1, H 3bO 3: 4.0mgL -1, MnCl 24H 2o:22mgL -1, Na 2moO 42H 2o:2.0mgL -1, NiCl 22H 2o:3.0mgL -1, ZnCl 2: 2.7mgL -1; Consisting of of nutritive salt substratum: NH 4n0 3: 1.7gL -1, K 2hPO 4: 1.4gL -1, MgCl 2: 0.22gL -1, CaCl 22H 2o:0.16gL -1;
(25) in mixed solution S, add 2000mL mixed solution Z, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid T;
(26) suspension liquid T is dried under 45 DEG C of conditions, can microbial organic fertilizer be obtained.
Bacterial strain germ oligotrophy unit cell Stenotrophomonas maltophilia TY-Cl used, on March 17th, 2015 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, this is centrally located at No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, and culture presevation number is CGMCC No.10636.
Described bacterial strain germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 is separated by the following method and obtains:
(1) choose wastewater from sugar plant pond bed mud 1000g to be positioned in 4 DEG C of refrigerators and to save backup;
(2) in open glass bottle, adding 500 ~ 600mL basic medium, is the bed mud 250g added after stirring 40 ~ 50min under the condition of 100r/min in step (1) at rotating speed; Then to be placed in temperature be the environment of 25 ~ 30 DEG C is stir acclimating under the condition of 100r/min to cultivate 10d at rotating speed, and the regular basic medium that adds in vial remains unchanged with the water and soil ratio maintaining mud system in vial in the training period; Wherein the consisting of of basic medium: NH 4cl:1.9gL -1, KH 2pO 4: 1.9gL -1, MgCl 2: 0.27gL -1, CaCl 22H 2o:0.13gL -1;
(4) to volume be 100 ~ 200mL Erlenmeyer flask in add the maltose solution that 20mL concentration is 5g/L;
(5) in the Erlenmeyer flask in step (4), 50mL basic medium, 0.5mL trace metal liquid and 0.1mL vitamin c solution is entered; Wherein the consisting of of trace metal liquid: CoCl 26H 2o:33mgL -1, CuCl 2: 0.26mgL -1, H 3bO 3: 4.0mgL -1, MnCl 24H 2o:22mgL -1, Na 2moO 42H 2o:2.0mgL -1, NiCl 22H 2o:3.0mgL -1, ZnCl 2: 2.7mgL -1;
(6) the bed mud 0.35g that access step (3) is tamed in the Erlenmeyer flask in step (5), then to be placed in temperature be 25 ~ 30 DEG C of rotating speeds is that the vibrator of 100r/min is cultivated 2 ~ 3 days, opens bottleneck every day once to recover the dissolved oxygen in Erlenmeyer flask; The ratio being 200 ~ 250: 1 in inoculation volume ratio proceeds to another by the Erlenmeyer flask after step (4) to (5) process, can obtain flora solution after switching number of times is greater than 4;
(7) to volume be 500mL Erlenmeyer flask in add 400mL basic medium, vitamin c solution that 1.0mL trace metal liquid, 0.1mL mass concentration are 1g/L, shake up rear for subsequent use as liquid nutrient medium;
(8) to volume be 150mL Erlenmeyer flask in add the liquid nutrient medium and 0.60g agar that 50mL prepares by step (7), then be sterilizing 20 minutes in the pressure kettle of 121 DEG C in temperature, when being at room temperature cooled to 65 ~ 70 DEG C, in Bechtop, be poured into volume is in the culture dish of 160mL, and this culture dish is placed 2 days by the moisture unnecessary for removing media surface in Bechtop;
(9) in Bechtop, add the maltose solution that 0.5mL concentration is 5g/L in the culture dish in step (8), tilt rotation culture dish makes maltose solution be uniformly distributed back and forth;
(10) to volume be 25mL Erlenmeyer flask in add the liquid nutrient medium and 0.25g agar that 10mL prepares by step (7), be sterilizing 20 minutes in the pressure kettle of 121 DEG C in temperature, in Bechtop, be cooled to 38 ~ 40 DEG C;
(11) in Bechtop, in the Erlenmeyer flask of step (10), add the flora solution that 1mL step (6) obtains, shaking up rear absorption 1mL slowly adds in the culture dish in step (9), and tilt rotation culture dish makes inoculation have the culture medium solution of mixed bacterial to be uniformly distributed back and forth; Culture dish is put into the incubator cultivation observation that temperature is 25 DEG C, just can find that there is obvious bacterium colony after 10 ~ 15 days and occur;
(12) bacterium colony in Bechtop in picking step (11) culture dish, carries out repeated isolation, and separation and purification more than 3 cycles can obtain pure strain.
The invention has the beneficial effects as follows, obtained microbial organic fertilizer has functions such as can discharging nitrogen, phosphorus, potassium, can be used in improvement soil, stimulates plant growth, improves agricultural product quality.
Embodiment
The present invention is further illustrated below in conjunction with example.
Be the filter cloth of 5mm by 8000mL sugar refinery waste liquid by aperture, be then aeration 2 hours under the condition of 150mL/min at gas flow, obtain mixed solution X; In mixed solution X, add 8mL trace metal liquid, under 1000r/min condition, stir 15min, obtain mixed solution Y, wherein the consisting of of trace metal liquid: CoCl 26H 2o:33mgL -1, CuCl 2: 0.26mgL -1, H 3bO 3: 4.0mgL -1, MnCl 24H 2o:22mgL -1, Na 2moO 42H 2o:2.0mgL -1, NiCl 22H 2o:3.0mgL -1, ZnCl 2: 2.7mgL -1; In mixed solution Y, add 48mL nutritive salt substratum, under 1000r/min condition, stir 15min, obtain mixed solution Z, wherein the consisting of of nutritive salt substratum: NH 4nO 3: 1.7gL -1, K 2hPO 4: 1.4gL -1, MgCl 2: 0.22gL -1, CaCl 22H 2o:0.16gL -1;
Be the NH of 0.25mol/L by 100mL concentration 4cl solution and 100mL concentration are the CaCl of 0.15mol/L 2solution, obtains mixed liquor A after mixing; Be (the NH of 0.15mol/L by 100mL concentration 4) 3pO 4solution is slowly added drop-wise in 200mL mixed liquor A under the condition stirred, and obtains mixed liquid B; In mixed liquid B, dropwise add the vitriol oil to clarification, obtain mixed solution C; Regulate the pH value of mixed solution C to be 3.5 with the hydrochloric acid soln of concentration to be the NaOH solution of 0.02mol/L and concentration be 0.02mol/L, obtain mixed solution D; In mixed solution D, add the aqueous solution of urea that 100mL concentration is 1.4mol/L, after mixing, obtain mixed solution E; In mixed solution E, add the calcio-disodium edetate solution that 50mL concentration is 0.25mol/L, after mixing, obtain mixed solution F;
By 60mL massfraction be 35% aqueous solution of urea be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution G in 35 minutes; By 10mL massfraction be 25% formalin be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution H in 35 minutes; In mixed solution G, drip the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L under 1000r/min agitation condition, the pH value making solution is 7.2 ~ 7.4, obtains mixed liquor I; Mixed solution H is joined in mixed liquor I under 1000r/min agitation condition, is then placed in 43 DEG C of water-baths and stirs 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution J; Under 1000r/min agitation condition, the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L is dripped in mixed solution J, the pH value of solution is made to be 3.7 ~ 3.9, then be placed in 38 DEG C of water-baths to stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution K;
1600mL deionized water is added drop-wise in mixed solution K under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution L; Be the KNO of 0.25mol/L by 100mL concentration 3solution and 100mL concentration are the Ca (NO of 1.55mol/L 3) 2solution fully mixes, and is then placed in 43 DEG C of water-bath constant temperature 35 minutes, obtains mixed solution M; 60mL mixed solution L is added drop-wise in mixed solution M under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution N; 60mL mixed solution F is added drop-wise in mixed solution N under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution O;
By 100mL massfraction be 5.5% sodium alginate soln be heated to 75 DEG C, then 100mL mixed solution O is added, 8min is stirred under 1000r/min condition, in 25 DEG C of water-baths, constant temperature crosses aperture after 15 hours is that solid particulate isolated by the sieve of 5mm, and then with deionized water wash 3 isolated solid particulates, lyophilize obtains solid particulate A; Solid particulate A is cultivated 36h in germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution, then take out and air-dryly obtain solid particulate B, wherein the bacteria containing amount of germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is 5 × 10 10cFU/ml, consisting of of nutrient solution: maltose 5gL -1, NH 4cl:1.9gL -1, KH 2pO 4: 1.9gL ~ 1, MgCl 2: 0.27gL -1, CaCl 22H 2o:0.13gL -1;
Solid particulate B is joined in 2000mL mixed solution Z, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid P; 2000mL mixed solution Z is joined in suspension liquid P under 1000r/min agitation condition, obtains mixed solution Q; In mixed solution Q, add 25mL mixed solution L, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R; 65mL nutritive salt substratum is added in suspension liquid Q, stir under 1000r/min condition after 5 minutes and add 25mL mixed solution F, and then add 2000mL mixed solution Z, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R; In suspension liquid R, add 4mL trace metal liquid, under 1000r/min condition, stir 15min, and then add 55mL nutritive salt substratum, obtain mixed solution S; In mixed solution S, add 2000mL mixed solution Z, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid T; Suspension liquid T is dried under 45 DEG C of conditions, can microbial organic fertilizer be obtained.
The fertilizer obtained has functions such as can discharging nitrogen, phosphorus, potassium, can be used in improvement soil, stimulates plant growth, improves agricultural product quality.

Claims (2)

1. with the production technique of sugar refinery waste liquid for raw material production microbial organic fertilizer, it is characterized in that, the concrete steps of described production technique are as follows:
(1) be the filter cloth of 5mm by 8000mL sugar refinery waste liquid by aperture, be then aeration 2 hours under the condition of 150mL/min at gas flow, obtain mixed solution X;
(2) in mixed solution X, add 8mL trace metal liquid, under 1000r/min condition, stir 15min, obtain mixed solution Y, wherein the consisting of of trace metal liquid: CoCl 26H 2o:33mgL -1, CuCl 2: 0.26mgL -1, H 3bO 3: 4.0mgL -1, MnCl 24H 2o:22mgL -1, Na 2moO 42H 2o:2.0mgL -1, NiCl 22H 2o:3.0mgL -1, ZnCl 2: 2.7mgL -1;
(3) in mixed solution Y, add 48mL nutritive salt substratum, under 1000r/min condition, stir 15min, obtain mixed solution Z, wherein the consisting of of nutritive salt substratum: NH 4nO 3: 1.7gL -1, K 2hPO 4: 1.4gL -1, MgCl 2: 0.22gL -1, CaCl 22H 2o:0.16gL -1;
(4) be the NH of 0.25mol/L by 100mL concentration 4cl solution and 100mL concentration are the CaCl of 0.15mol/L 2solution, obtains mixed liquor A after mixing;
(5) be (NH of 0.15mol/L by 100mL concentration 4) 3pO 4solution is slowly added drop-wise in 200mL mixed liquor A under the condition stirred, and obtains mixed liquid B;
(6) in mixed liquid B, dropwise add the vitriol oil to clarification, obtain mixed solution C;
(7) regulate the pH value of mixed solution C to be 3.5 with the hydrochloric acid soln of concentration to be the NaOH solution of 0.02mol/L and concentration be 0.02mol/L, obtain mixed solution D;
(8) in mixed solution D, add the aqueous solution of urea that 100mL concentration is 1.4mol/L, after mixing, obtain mixed solution E;
(9) in mixed solution E, add the calcio-disodium edetate solution that 50mL concentration is 0.25mol/L, after mixing, obtain mixed solution F;
(10) by 60mL massfraction be 35% aqueous solution of urea be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution G in 35 minutes; By 10mL massfraction be 25% formalin be placed in 43 DEG C of water-bath constant temperature and obtain mixed solution H in 35 minutes;
(11) in mixed solution G, drip the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L under 1000r/min agitation condition, the pH value making solution is 7.2 ~ 7.4, obtains mixed liquor I;
(12) mixed solution H is joined in mixed liquor I under 1000r/min agitation condition, be then placed in 43 DEG C of water-baths and stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution J;
(13) in mixed solution J, under 1000r/min agitation condition, drip the HCl solution that the NaOH solution that concentration is 0.01mol/L and concentration are 0.005mol/L, the pH value of solution is made to be 3.7 ~ 3.9, then be placed in 38 DEG C of water-baths to stir 90 ~ 100 minutes under 1000r/min condition, obtain mixed solution K;
(14) 1600mL deionized water is added drop-wise in mixed solution K under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution L;
(15) be the KNO of 0.25mol/L by 100mL concentration 3solution and 100mL concentration are the Ca (NO of 1.55mol/L 3) 2solution fully mixes, and is then placed in 43 DEG C of water-bath constant temperature 35 minutes, obtains mixed solution M;
(16) 60mL mixed solution L is added drop-wise in mixed solution M under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution N;
(17) 60mL mixed solution F is added drop-wise in mixed solution N under 1000r/min agitation condition, then under 1000r/min condition, stirs 8min, obtain mixed solution O;
(18) by 100mL massfraction be 5.5% sodium alginate soln be heated to 75 DEG C, then 100mL mixed solution O is added, 8min is stirred under 1000r/min condition, in 25 DEG C of water-baths, constant temperature crosses aperture after 15 hours is that solid particulate isolated by the sieve of 5mm, and then with deionized water wash 3 isolated solid particulates, lyophilize obtains solid particulate A;
(19) solid particulate A is cultivated 36h in germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution, then take out and air-dryly obtain solid particulate B, wherein the bacteria containing amount of germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 nutrient solution is 5 × 10 10cFU/ml, consisting of of nutrient solution: maltose 5gL -1, NH 4cl:1.9gL -1, KH 2pO 4: 1.9gL -1, MgCl 2: 0.27gL -1, CaCl 22H 2o:0.13gL -1;
(20) solid particulate B is joined in 2000mL mixed solution Z, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid P;
(21) 2000mL mixed solution Z is joined in suspension liquid P under 1000r/min agitation condition, obtain mixed solution Q;
(22) in mixed solution Q, add 25mL mixed solution L, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R;
(23) in suspension liquid Q, 65mL nutritive salt substratum is added, stir under 1000r/min condition after 5 minutes and add 25mL mixed solution F, and then add 2000mL mixed solution Z, it is aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid R; Wherein the consisting of of nutritive salt substratum: NH 4nO 3: 1.7gL -1, K 2hPO 4: 1.4gL -1, MgCl 2: 0.22gL -1, CaCl 22H 2o:0.16gL -1;
(24) in suspension liquid R, add 4mL trace metal liquid, under 1000r/min condition, stir 15min, and then add 55mL nutritive salt substratum, obtain mixed solution S, wherein the consisting of of trace metal liquid: CoCl 26H 2o:33mgL -1, CuCl 2: 0.26mgL -1, H 3bO 3: 4.0mgL -1, MnCl 24H 2o:22mgL -1, Na 2moO 42H 2o:2.0mgL -1, NiCl 22H 2o:3.0mgL -1, ZnCl 2: 2.7mgL -1; Consisting of of nutritive salt substratum: NH 4nO 3: 1.7gL -1, K 2hPO 4: 1.4gL -1, MgCl 2: 0.22gL -1, CaCl 22H 2o:0.16gL -1;
(25) in mixed solution S, add 2000mL mixed solution Z, be aeration 15 hours under the condition of 220mL/min at gas flow, discharge supernatant liquor after staticly settling 3 hours, obtain suspension liquid T;
(26) suspension liquid T is dried under 45 DEG C of conditions, can microbial organic fertilizer be obtained.
2. according to claim 1 with the production technique of sugar refinery waste liquid for raw material production microbial organic fertilizer, it is characterized in that, the bacterial strain germ oligotrophy unit cell Stenotrophomonas maltophilia TY-C1 applied in described production technique, on March 17th, 2015 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, this is centrally located at No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, and culture presevation number is CGMCC No.10636.
CN201510172463.0A 2015-04-14 2015-04-14 Process for producing microbial organic fertilizer by taking waste liquid from sugar refinery as raw material Pending CN104945081A (en)

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CN111362737A (en) * 2018-12-25 2020-07-03 四川绿高生态农业科技开发有限公司 Method for producing microbial organic fertilizer by taking cultivation waste as raw material
CN111434644A (en) * 2018-12-25 2020-07-21 四川绿高生态农业科技开发有限公司 Preparation method of composite microbial organic liquid fertilizer suitable for soil heavy metal remediation

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