CN104940294A - Preparation method for punicosides, and method for preparing punicosides microcapsule containing punicosides, prepared according to preparation method, and microcapsule - Google Patents
Preparation method for punicosides, and method for preparing punicosides microcapsule containing punicosides, prepared according to preparation method, and microcapsule Download PDFInfo
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- CN104940294A CN104940294A CN201510342170.2A CN201510342170A CN104940294A CN 104940294 A CN104940294 A CN 104940294A CN 201510342170 A CN201510342170 A CN 201510342170A CN 104940294 A CN104940294 A CN 104940294A
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Abstract
The invention relates to a preparation method for punicosides, and a method for preparing a punicosides microcapsule containing punicosides, prepared according to preparation method, and a microcapsule. The preparation method comprises the following steps: after pomegranate bark is ground, conducting alcohol extraction, performing rotary evaporation and concentration after the alcohol extract is filtered by a film, and finally carrying out freeze drying on the concentrated solution. The preparation method and the method have the benefits that the defects of high possibility of oxidizing punicosides and low stability are overcome, the bioactivity of punicosides is remained effectively, and the bioavailability in a normal environment is improved. The problem that biologically active components are high in possibility of oxidizing and low in stability during the application process is solved, the storage period of punicosides is prolonged greatly, and the possibility of application of various punicosides products is improved.
Description
Technical field
The present invention relates to the extraction of effective components in plants and the preparing technical field of health product, particularly a kind of preparation method of Punica granatum L. polyphenol and the Punica granatum L. polyphenol microcapsule of Punica granatum L. polyphenol prepared containing the method and the preparation method of microcapsule.
Background technology
Punica granatum L. (Punicagranatum) polyphenol is the general name that a class has polyphenol hydroxyl compound, comprise the multiple compounds such as elladitannin, Galla Turcica (Galla Helepensis) tannin, ellagic acid, gallic acid, catechin, anthocyanidin, chlorogenic acid, ferulic acid and quercetin, mainly be present in Pericarpium Granati, content is about 10% ~ 20% of its dry mass, has antioxidation, defying age, anti-cancer and cancer-preventing, antibacterial, multiple physiology and the pharmacologically active such as moistening skin beautifying, blood pressure lowering and prevention cardiovascular and cerebrovascular disease.Non-oxidizability and the antibacterial activity of Punica granatum L. polyphenol are higher, demonstrate huge using value in fields such as food, medicine and household chemicalss.At present to the application to Punica granatum L. industrialization, multiplexly preparing Sucus Granati, traditional juice drinks such as Pomegranate vinegar, is also the important subject of Punica granatum L. industry higher value application by the active component extracted in Pericarpium Granati.The report that extracts of the domestic and international polyphenol to Pericarpium Granati is fewer at present, existing method mostly is decocting method, this method has the features such as easy and simple to handle and cost is low, but easily cause the destruction of Polyphenols and other antioxidant contents in Pericarpium Granati, and impurity content is higher in extracting solution, be unfavorable for the isolation and purification of polyphenol.Therefore, the method for the autoxidation of Punica granatum L. polyphenol in leaching process is avoided in necessary searching, ensures the stability of its activity with it.
Phenolic hydroxyl group activity contained in polyphenol molecule is very strong, and be very easily subject to the impact of the environmental conditions such as high temperature, humidity, illumination, be easily polymerized, be oxidized, condensation etc. is reacted and lost the biological activitys such as antioxidation.Polyphenol substance is powdered mostly, and granularity is very thin, and easier moisture absorption caking in natural environment, thus isolated polyphenol substance, in storage, transport and application process, incident Oxidation can reduce its result of use greatly.
Micro encapsulation is embedded into by solid, liquid, gas state material in small, semi permeability or closed capsule, makes content under given conditions with the technology of controlled speed release.This small closed capsule and microcapsule, the process of parcel and micro encapsulation.By the method for microcapsule embedded polyphenol, the original chemical characteristic of goal object and biological nature at all be can't harm, then discharged by some outside stimulus or slow releasing function gradually.Also not about the concrete introduction of the preparation method of Punica granatum L. polyphenol microcapsule in existing report.
Summary of the invention
The present invention proposes a kind of preparation method of Punica granatum L. polyphenol and a kind of Punica granatum L. polyphenol microcapsule of Punica granatum L. polyphenol of preparing containing the method and the preparation method of this microcapsule.
A preparation method for Punica granatum L. polyphenol, comprises following steps:
(1) fresh pomegranate is dried rear pulverizing, cross 20 mesh sieves, afterwards Punica granatum L. corium farinosum is added in the alcoholic solution of 10-15 times of weight and extract 2-3 hour;
(2) extract 3 times as stated above, merge extractive liquid;
(3) by said extracted liquid through molecular cut off be the membrance separation of 60,000-10 ten thousand Da;
(4) molecular weight obtained by said method is that the Punica granatum L. polyphenol extracting solution between 60,000-10 ten thousand Da is placed in vacuum rotary evaporator, and rotary evaporation, concentrated volume is behind 1/3 to 1/2 of original volume, and lyophilization obtains Punica granatum L. polyphenol powder extract.
On the basis of such scheme, the volume fraction of the alcoholic solution in step 1 is 20%-50%; Extracting temperature is 40-65 DEG C.
On the basis of such scheme, the temperature of the rotary evaporation in step 4 is 30-40 DEG C, and vacuum is-0.05MPa-0.1MPa; Lyophilization is vacuum lyophilization, temperature :-10 DEG C, 48h.
A kind of Punica granatum L. polyphenol microcapsule, employs Punica granatum L. polyphenol prepared by above-mentioned method, by weight ratio be 1: 2-4 Punica granatum L. polyphenol and wall material form.
Described wall material is: one or more in maltodextrin, beta-schardinger dextrin-, sodium caseinate and gelatin.
A preparation method for Punica granatum L. polyphenol microcapsule as above, concrete steps are as follows:
(1) Punica granatum L. polyphenol is added anhydrous alcohol solution;
(2) wall material is added in pure water, fully stir.
(3) Punica granatum L. polyphenol solution is joined in wall material solution, be uniformly mixed and be placed in high speed disperser; Under 50 DEG C of conditions, with the rotating speed of 10000r/min dispersion 15min;
(4) by step 3) mixed liquor that obtains is placed in agitator, and stir 25min with the rotating speed of 900r/min under 50 DEG C of conditions, obtain Punica granatum L. polyphenol microcapsule solution;
(5) by the Punica granatum L. polyphenol microcapsule solution of gained inlet temperature 180 DEG C, spraying dry under leaving air temp 70 DEG C of conditions, namely obtains Punica granatum L. polyphenol microcapsule.
Beneficial effect of the present invention:
By the extraction process of Punica granatum L. polyphenol in the middle of the present invention, making the active component remained largely in the middle of Punica granatum L. polyphenol, is that the extracted amount of Punica granatum L. polyphenol is maximum.In addition, the Punica granatum L. polyphenol after extraction can be applied to the research and development raw material of other Related products.
By the micro encapsulation of Punica granatum L. polyphenol, the feature of oxidizable, the poor stability solving that polyphenol faces.Effectively maintain the biological activity of polyphenol, improve the bioavailability under normal environment.In addition, the cost of wall material is relatively low, save the cost preserving Punica granatum L. polyphenol largely, the more easily preservation at normal temperatures of microcapsule after preparation, not easily by the air oxidation in the external world, microcapsule also to some extent solves the problem of polyphenol substance in the uneven instability of food system distribution again in addition, largely for the further exploitation of product provides the foundation.To some extent solve the problem of biological active component oxidizable instability in application process, greatly extend the preservation term of Punica granatum L. polyphenol, improve the probability of each product form application of Punica granatum L. polyphenol.
Detailed description of the invention
Following examples are only limitted to set forth the present invention, and protection scope of the present invention is not limited only to following examples.
Embodiment 1
The extracting method of Punica granatum L. polyphenol
(1) fresh pomegranate is dried rear pulverizing, cross 20 mesh sieves, adding weight is its alcoholic solution of 10 times.In solution, the volume fraction of ethanol is 50%.
(2) adopt mixed extraction 2h under 40 DEG C of conditions, extract 3 times, merge extractive liquid.
(3) by above-mentioned mixed extract through molecular cut off be the membrance separation of 60,000-10 ten thousand Da, obtaining molecular weight is Punica granatum L. polyphenol extracting solution between 60,000-10 ten thousand Da.
(4) be placed in vacuum rotary evaporator by said extracted liquid, rotary evaporation at 30 DEG C, vacuum is-0.05MPa, and concentrated volume is 1/3 of original volume, and lyophilization (-10 DEG C, 48h) obtains Punica granatum L. polyphenol powder extract.
Embodiment 2
The extracting method of Punica granatum L. polyphenol
(1) fresh pomegranate is dried rear pulverizing, cross 20 mesh sieves, adding weight is its alcoholic solution of 15 times.In solution, the volume fraction of ethanol is 20%.
(2) adopt mixed extraction 3h under 65 DEG C of conditions, extract 3 times, merge extractive liquid.
(3) by above-mentioned mixed extract through molecular cut off be the membrance separation of 60,000-10 ten thousand Da, obtaining molecular weight is Punica granatum L. polyphenol extracting solution between 60,000-10 ten thousand Da.
(4) be placed in vacuum rotary evaporator by said extracted liquid, rotary evaporation at 40 DEG C, vacuum is 0.1MPa, and concentrated volume is 1/2 of original volume, and lyophilization (-10 DEG C, 48h) obtains Punica granatum L. polyphenol powder extract.
Embodiment 3
(1) get 1g Punica granatum L. polyphenol, add 8ml anhydrous alcohol solution, then add 12ml pure water and fully dissolve, obtain polyphenol solution (core).
(2) get 50ml, the pure water of 40 DEG C, add 3g maltodextrin, stirring and dissolving 10min, and then add 0.3g sodium caseinate, be uniformly mixed 40min, obtain compound wall materials liquid.
(3) Punica granatum L. polyphenol solution is joined in wall material solution, be uniformly mixed and be placed in high speed disperser; Under 50 DEG C of conditions, with the rotating speed of 12000r/min dispersion 15min.
(4) then take out, be placed in agitator, stir 30min with the rotating speed of 1000r/min under 50 DEG C of conditions, obtain Punica granatum L. polyphenol microcapsule solution.By the Punica granatum L. polyphenol microcapsule solution of gained inlet temperature 180 DEG C, spraying dry under leaving air temp 70 DEG C of conditions, namely obtains Punica granatum L. polyphenol microcapsule.
Embodiment 4
(1) get 2g Punica granatum L. polyphenol, add 10ml anhydrous alcohol solution, then add 20ml pure water and fully dissolve, obtain polyphenol solution (core).
(2) get 25ml, the pure water of 40 DEG C, add 5g gelatin, stirring and dissolving 10min, and then add 0.3g sodium caseinate and 0.7g beta-schardinger dextrin-, be uniformly mixed 40min, obtain compound wall materials liquid.
(3) Punica granatum L. polyphenol solution is joined in wall material solution, be uniformly mixed and be placed in high speed disperser; Under 50 DEG C of conditions, with the rotating speed of 10000r/min dispersion 15min.
(4) then take out, be placed in agitator, stir 25min with the rotating speed of 900r/min under 50 DEG C of conditions, obtain Punica granatum L. polyphenol microcapsule solution.By the Punica granatum L. polyphenol microcapsule solution of gained inlet temperature 180 DEG C, spraying dry under leaving air temp 70 DEG C of conditions, namely obtains Punica granatum L. polyphenol microcapsule.
The preferred embodiment of the present invention is illustrative, and scope of the present invention is not defined in these embodiments, and scope of the present invention is determined by claims, comprises and belonging to and the multiple amendment of equivalency range of the present invention and distortion.
Claims (8)
1. a preparation method for Punica granatum L. polyphenol, is characterized in that, comprises following steps:
Alcohol extraction after being pulverized by Pericarpium Granati, alcohol extract is concentrated by rotary evaporation after membrane filtration, finally by concentrated solution lyophilization.
2. the preparation method of Punica granatum L. polyphenol according to claim 1, is characterized in that, the film that membrane filtration uses for molecular cut off be the film of 60,000-10 ten thousand Da.
3. the preparation method of Punica granatum L. polyphenol according to claim 1, is characterized in that, concrete step is as follows:
(1) fresh pomegranate is dried rear pulverizing, cross 20 mesh sieves, afterwards Punica granatum L. corium farinosum is added in the alcoholic solution of 10-15 times of weight and extract 2-3 hour;
(2) extract 3 times as stated above, merge extractive liquid;
(3) by said extracted liquid through molecular cut off be the membrance separation of 60,000-10 ten thousand Da;
(4) molecular weight obtained by said method is that the Punica granatum L. polyphenol extracting solution between 60,000-10 ten thousand Da is placed in vacuum rotary evaporator, and rotary evaporation, concentrated volume is behind 1/3 to 1/2 of original volume, and lyophilization obtains Punica granatum L. polyphenol powder extract.
4. the preparation method of Punica granatum L. polyphenol according to claim 3, is characterized in that: the volume fraction of the alcoholic solution in step (1) is 20%-50%; Extracting temperature is 40-65 DEG C.
5. the preparation method of Punica granatum L. polyphenol according to claim 4, is characterized in that: the temperature of the rotary evaporation in step (4) is 30-40 DEG C, and vacuum is-0.05MPa-0.1MPa; Lyophilization is vacuum lyophilization, temperature :-10 DEG C, 48h.
6. a Punica granatum L. polyphenol microcapsule, is characterized in that, employs Punica granatum L. polyphenol prepared by the method described in any one of claim 1-5, by weight ratio be 1: 2-4 Punica granatum L. polyphenol and wall material form.
7. Punica granatum L. polyphenol microcapsule according to claim 6, is characterized in that: described wall material is: one or more in maltodextrin, beta-schardinger dextrin-, sodium caseinate and gelatin.
8. a preparation method for the Punica granatum L. polyphenol microcapsule as described in claim 7 and 8, is characterized in that: concrete steps are as follows:
(1) Punica granatum L. polyphenol is added anhydrous alcohol solution;
(2) wall material is added in pure water, fully stir.
(3) Punica granatum L. polyphenol solution is joined in wall material solution, be uniformly mixed and be placed in high speed disperser; Under 50 DEG C of conditions, with the rotating speed of 10000-12000r/min dispersion 15min;
(4) by step 3) mixed liquor that obtains is placed in agitator, and stir 25-30min with the rotating speed of 900-1000r/min under 50 DEG C of conditions, obtain Punica granatum L. polyphenol microcapsule solution;
(5) by the Punica granatum L. polyphenol microcapsule solution of gained inlet temperature 180 DEG C, spraying dry under leaving air temp 70 DEG C of conditions, namely obtains Punica granatum L. polyphenol microcapsule.
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| CN201510342170.2A CN104940294A (en) | 2015-06-16 | 2015-06-16 | Preparation method for punicosides, and method for preparing punicosides microcapsule containing punicosides, prepared according to preparation method, and microcapsule |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105435723A (en) * | 2015-11-26 | 2016-03-30 | 江苏大学 | Toilet soap containing pomegranate polyphenol microcapsule and preparation method of pomegranate polyphenol microcapsule |
| CN106518590A (en) * | 2016-09-18 | 2017-03-22 | 曲阜师范大学 | Method for extracting polyphenol substances from pomegranate peel by using low eutectic solvent |
| CN106666739A (en) * | 2016-12-19 | 2017-05-17 | 黄腾庆 | Preparation method of pomegranate polyphenols |
| CN106690154A (en) * | 2016-11-22 | 2017-05-24 | 张海生 | Processing method of red jujube polyphenol microcapsule food |
| CN113940429A (en) * | 2021-11-08 | 2022-01-18 | 陕西师范大学 | Pomegranate peel residue polyphenol microcapsule and preparation method thereof |
| CN114009267A (en) * | 2021-11-02 | 2022-02-08 | 合肥康来生态农业有限公司 | Pomegranate planting method rich in polyphenol |
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| CN101766670A (en) * | 2010-02-23 | 2010-07-07 | 上海应用技术学院 | Rhodiola rosea polyphenol microcapsule and preparation method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105435723A (en) * | 2015-11-26 | 2016-03-30 | 江苏大学 | Toilet soap containing pomegranate polyphenol microcapsule and preparation method of pomegranate polyphenol microcapsule |
| CN106518590A (en) * | 2016-09-18 | 2017-03-22 | 曲阜师范大学 | Method for extracting polyphenol substances from pomegranate peel by using low eutectic solvent |
| CN106690154A (en) * | 2016-11-22 | 2017-05-24 | 张海生 | Processing method of red jujube polyphenol microcapsule food |
| CN106666739A (en) * | 2016-12-19 | 2017-05-17 | 黄腾庆 | Preparation method of pomegranate polyphenols |
| CN114009267A (en) * | 2021-11-02 | 2022-02-08 | 合肥康来生态农业有限公司 | Pomegranate planting method rich in polyphenol |
| CN113940429A (en) * | 2021-11-08 | 2022-01-18 | 陕西师范大学 | Pomegranate peel residue polyphenol microcapsule and preparation method thereof |
| CN113940429B (en) * | 2021-11-08 | 2024-01-09 | 陕西师范大学 | Pomegranate rind residue polyphenol microcapsule and preparation method thereof |
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Application publication date: 20150930 |