CN103450366B - The method of the water-soluble and caustic solubility tea polysaccharide mixture of a kind of comprehensive preparation - Google Patents
The method of the water-soluble and caustic solubility tea polysaccharide mixture of a kind of comprehensive preparation Download PDFInfo
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Abstract
The method of the water-soluble and caustic solubility tea polysaccharide mixture of a kind of comprehensive preparation.Water is sprayed in tea raw material, be then placed in Bulking tank, apply 0.10 ~ 0.90? MPa pressure, regulates temperature to be 60 ~ 110 DEG C, carries out pressure treatment, hold-time 3 ~ 50min; Open pressure relief system, by pressure drop to 0.01 ~ 0.07 in tank? MPa, regulates temperature to be 40 ~ 90 DEG C, hold-time 5 ~ 90min, obtains the tea raw material of changing temperature-pressure-difference and puffing process.By this tea raw material, extracting solution after hot water extraction, take final concentration as the papoid deproteinated of 0.001-0.8%, then be the hydrogen peroxide for decoloration of 0.05-4.0% with final concentration, with molecular weight cut-off be 1000-6000 dalton ultra-filtration membrane concentrate, concentrated solution adopts alcohol settling, drying precipitate, obtains water soluble tea polysaccharide compound; Tea grounds after water soluble tea polysaccharide compound extracts adds the sodium bicarbonate water that final concentration is 0.1-8.0%, filter, filtered liquid adds the stomach en-deproteinated that final concentration is 0.001-0.9%, again with the hydrogen peroxide for decoloration of 0.04-4.5%, with membrane pore size molecular weight cut-off be the daltonian ultra-filtration membrane of 1000-6000 concentrate, concentrated solution is through alcohol settling, and drying precipitate, obtains caustic solubility tea polysaccharide mixture.
Description
Technical field
The present invention relates to and a kind ofly comprehensively from tealeaves extract the technological method preparing water soluble tea polysaccharide compound and caustic solubility tea polysaccharide mixture, belong to field of deep processing of farm products.
Background technology
Tea Polysaccharides is green tea, oolong tea, black tea, black tea, a kind of mixed polysaccharide of conjugated protein that contains in the white teas such as tea and yellow tea, full name is tea polysaccharide mixture (teapolysaccharideconjugates, TPC), its polysaccharide chain part is formed by monose covalent attachment such as mouse pectinose, wood sugar, Lee's sugar, seminose, glucose, semi-lactosi, galacturonic acid and glucuronic acids; Tens seed amino acids form the peptide chain portion of its conjugated protein.Tea polysaccharide mixture hypoglycemic, strengthen immunity and anti-oxidant etc. in there is remarkable function, be biologically active substance important in tealeaves.
Tea polysaccharide mixture has research and development utility value and market outlook preferably.At present, because the content of tea polysaccharide mixture in dry tea is lower, be generally 2-4%, therefore in its leaching process, the main high temperature that adopts decocts, to ensure its yield.But the tea polysaccharide of high temperature extraction has problems in biological activity and purity; In addition, still main extraction and application water soluble tea polysaccharide at present, its extract after tea grounds then abandoned, but in tea grounds, have a large amount of caustic solubility tea polysaccharide mixtures, equally there is the effects such as hypoglycemic.As taked pre-treatment before extraction to tea raw material, it is made significantly to improve the yield of target product, the caustic solubility tea polysaccharide mixture of going forward side by side in onestep extraction tea grounds, thus make use of the polysaccharide resource in tealeaves to greatest extent, the deep development for tea polysaccharide utilizes and lays the foundation.
Summary of the invention
In order to solve the problem, the invention provides a kind of changing temperature-pressure-difference and puffing technical finesse tea raw material, the tea grounds after hot water extraction water soluble tea polysaccharide compound adopts sodium bicarbonate to extract the method for caustic solubility tea polysaccharide mixture.The technical solution used in the present invention comprises the steps:
(1) tea raw material changing temperature-pressure-difference and puffing process: water is sprayed the tea raw material in certain mass, injection flow rate is 3.0 ~ 50.0% of tea quality, this moisture tea raw material is placed in Bulking tank, apply 0.10 ~ 0.90MPa pressure, temperature is regulated to be 60 ~ 110 DEG C, carry out pressure treatment, hold-time 3 ~ 50min.Then open pressure relief system, by pressure drop to 0.01 in tank ~ 0.07MPa, regulate temperature to be 40 ~ 90 DEG C, hold-time 5 ~ 90min, obtain the tea raw material of changing temperature-pressure-difference and puffing process.
(2) water soluble tea polysaccharide compound extracts the tea raw material of above-mentioned changing temperature-pressure-difference and puffing process, add its quality 5-30 water doubly, 0.5-12 hour is extracted in 40-95 DEG C, then filtered through gauze is used, the use of filter residue caustic solubility tea polysaccharide to be extracted mixture, after filtrate is centrifugal, concentrate with decompression rotary evaporation, concentrated solution adds papoid and carries out deproteinated, the final concentration of proteolytic enzyme is made to be 0.001-0.8%, then food grade hydrogen peroxide for decoloration is added, its final concentration is made to be 0.05-4.0%, decolour, concentrated solution after decolouring adopts membrane pore size molecular weight cut-off to be 1000-6000 dalton ultrafiltration membrance filter, trapped fluid adds 4 times of volume 95% ethanol again, leave standstill 2-12 hour, obtain ethanol pellet, by throw out vacuum lyophilization or spraying dry, obtain water soluble tea polysaccharide compound.
(3) the extraction food grade sodium bicarbonate preparation mass percent concentration of caustic solubility tea polysaccharide mixture is the sodium bicarbonate water of 0.1-8.0%.Sodium bicarbonate water is added in the filter residue described in step (2), the quality of added sodium bicarbonate water is 2-25 times of the tea raw material quality of puffing, and extract 0.5-10 hour in 30-90 DEG C, then filtered through gauze is used, after filtrate is centrifugal, concentrate with decompression rotary evaporation, concentrated solution adds stomach en-, make the final concentration of proteolytic enzyme be 0.001-0.9%, carry out deproteinated; Then food grade hydrogen peroxide for decoloration is added, its final concentration is made to be 0.04-4.5%, decolour, concentrated solution after decolouring adopts membrane pore size molecular weight cut-off to be 1000-6000 dalton ultrafiltration membrance filter, trapped fluid adds 4 times of volume 95% ethanol again, leaves standstill 2-12 hour, obtains ethanol pellet, by throw out vacuum lyophilization or spraying dry, obtain caustic solubility Tea Polysaccharides mixture.
Tea raw material in described step (1) is any one in green tea, white tea, yellow tea, oolong tea (blue or green tea), black tea, black tea, especially using low-grade tealeaves as the raw material extracting tea polysaccharide mixture.
Beneficial effect of the present invention is, the method technical process is simple, significantly improves the yield of tea polysaccharide mixture, and does not adopt virulent organic solvent, environmental protection; In abundant exploitation tealeaves, water-soluble and caustic solubility tea polysaccharide mixture, with low cost, is easy to preparation of industrialization.
Claims (2)
1. comprehensively prepare a method that is water-soluble and caustic solubility tea polysaccharide mixture, it is characterized in that comprising the following steps:
Step 1 tea raw material changing temperature-pressure-difference and puffing process: water is sprayed the tea raw material in certain mass, injection flow rate is 3.0 ~ 50.0% of tea quality, this moisture tea raw material is placed in Bulking tank, apply 0.10 ~ 0.90MPa pressure, temperature is regulated to be 60 ~ 110 DEG C, carry out pressure treatment, hold-time 3 ~ 50min, then pressure relief system is opened, by pressure drop to 0.01 in tank ~ 0.07MPa, regulate temperature to be 40 ~ 90 DEG C, hold-time 5 ~ 90min, obtain the tea raw material of changing temperature-pressure-difference and puffing process;
Step 2 water soluble tea polysaccharide compound extracts: by the tea raw material of above-mentioned changing temperature-pressure-difference and puffing process, add its quality 5-30 water doubly, 0.5-12 hour is extracted in 40-95 DEG C, then filtered through gauze is used, the use of filter residue caustic solubility tea polysaccharide to be extracted mixture, after filtrate is centrifugal, concentrate with decompression rotary evaporation, concentrated solution adds papoid and carries out deproteinated, the final concentration of proteolytic enzyme is made to be 0.001-0.8%, then food grade hydrogen peroxide for decoloration is added, its final concentration is made to be 0.05-4.0%, decolour, concentrated solution after decolouring adopts membrane pore size molecular weight cut-off to be 1000-6000 dalton ultrafiltration membrance filter, trapped fluid adds 4 times of volume 95% ethanol again, leave standstill 2-12 hour, obtain ethanol pellet, by throw out vacuum lyophilization or spraying dry, obtain water soluble tea polysaccharide compound,
The extraction of step 3 caustic solubility tea polysaccharide mixture: the sodium bicarbonate water with food grade sodium bicarbonate preparation mass percent concentration being 0.1-8.0%, sodium bicarbonate water is added in the filter residue described in step (2), the quality of added sodium bicarbonate water is 2-25 times of the tea raw material quality of puffing, and extract 0.5-10 hour in 30-90 DEG C, then filtered through gauze is used, after filtrate is centrifugal, concentrate with decompression rotary evaporation, concentrated solution adds stomach en-, the final concentration of proteolytic enzyme is made to be 0.001-0.9%, carry out deproteinated, then food grade hydrogen peroxide for decoloration is added, its final concentration is made to be 0.04-4.5%, decolour, concentrated solution after decolouring adopts membrane pore size molecular weight cut-off to be 1000-6000 dalton ultrafiltration membrance filter, trapped fluid adds 4 times of volume 95% ethanol again, leave standstill 2-12 hour, obtain ethanol pellet, by throw out vacuum lyophilization or spraying dry, obtain caustic solubility Tea Polysaccharides mixture.
2. method according to claim 1, is characterized in that tea raw material is any one in green tea, white tea, yellow tea, oolong tea (blue or green tea), black tea, black tea.
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| CN103960400B (en) * | 2014-02-25 | 2016-06-29 | 中华全国供销合作总社杭州茶叶研究所 | A kind of preparation method of instant type condensed juice of green tea |
| CN104629895A (en) * | 2015-02-09 | 2015-05-20 | 吴海军 | Cucumis metuliferus fruit seed oil and preparation method thereof |
| CN104621495B (en) * | 2015-02-26 | 2017-12-26 | 广东一家亲营养科技有限公司 | A kind of dragon fruit pericarp activity food fibre powder and preparation method thereof |
| CN105367679A (en) * | 2015-12-03 | 2016-03-02 | 镇江市丹徒区南山溪园茶叶专业合作社 | Green tea polysaccharide extraction method |
| CN107312105A (en) * | 2017-07-27 | 2017-11-03 | 福建农林大学 | A kind of Tea Polysaccharides chromic compound and its preparation and application |
| CN107397225A (en) * | 2017-09-20 | 2017-11-28 | 贵州苗贵客贸易发展有限公司 | A kind of extracting method of green-tea extract |
| CN109566929B (en) * | 2018-10-23 | 2022-02-22 | 长春大学 | Preparation method of dandelion health-care beverage |
| CN110755552B (en) * | 2019-11-29 | 2021-11-23 | 贵州天诚农业科技发展有限公司 | Preparation method of herba dendrobii powder |
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| CN1379044A (en) * | 2002-03-11 | 2002-11-13 | 上海中药创新研究中心 | Purified theopolyose and its extracting process |
| CN101695326A (en) * | 2009-10-29 | 2010-04-21 | 天津农学院 | Method for preparing cold-brewing tea by puffing at variable temperature and pressure differences |
| CN101798356A (en) * | 2010-04-08 | 2010-08-11 | 晋江市恒源科技开发有限公司 | Method for separating and extracting natural tea polysaccharide |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379044A (en) * | 2002-03-11 | 2002-11-13 | 上海中药创新研究中心 | Purified theopolyose and its extracting process |
| CN101695326A (en) * | 2009-10-29 | 2010-04-21 | 天津农学院 | Method for preparing cold-brewing tea by puffing at variable temperature and pressure differences |
| CN101798356A (en) * | 2010-04-08 | 2010-08-11 | 晋江市恒源科技开发有限公司 | Method for separating and extracting natural tea polysaccharide |
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