CN103450366A - Method for comprehensively preparing water-soluble and alkali-solution tea polysaccharide composite - Google Patents
Method for comprehensively preparing water-soluble and alkali-solution tea polysaccharide composite Download PDFInfo
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- CN103450366A CN103450366A CN2012102529311A CN201210252931A CN103450366A CN 103450366 A CN103450366 A CN 103450366A CN 2012102529311 A CN2012102529311 A CN 2012102529311A CN 201210252931 A CN201210252931 A CN 201210252931A CN 103450366 A CN103450366 A CN 103450366A
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Abstract
The invention relates to a method for comprehensively preparing a water-soluble and alkali-solution tea polysaccharide composite. The method comprises the following steps of: spraying water onto tea leaves, then placing in a swelling tank, applying 0.10-0.90MPa pressure, regulating the temperature to be 60-110 DEG C, performing pressurization, and keeping for 3-50 minutes; starting a decompression system, reducing the pressure in the tank to 0.01-0.07MPa, regulating the temperature to be 40-90 DEG C, and keeping for 5-90 minutes to obtain variable-temperature differential pressure swelled tea leaves; extracting the tea leaves with hot water to obtain an extracting solution, deproteinizing by use of papain with final concentration of 0.001-0.8%, decolorizing by use of hydrogen peroxide with final concentration of 0.05-4.0%, concentrating by use of an ultra-filtration membrane with molecular weight cutoff of 1,000-6,000 daltons, precipitating the concentrated liquor through ethanol, drying the precipitate to obtain a water-soluble tea polysaccharide composite; adding a baking soda solution with final concentration of 0.1-8.0% into tea grounds obtained by extracting the water-soluble tea polysaccharide composite, filtering, adding pepsin with final concentration of 0.001-0.9% into the filtrate so as to carry out deproteinizing, then decolorizing by use of hydrogen peroxide with final concentration of 0.04-4.5%, concentrating through an ultra-filtration membrane with membrane pore size molecular weight cutoff of 1,000-6,000 daltons, precipitating the concentrated liquor through ethanol, and drying the precipitate to obtain the alkali-soluble tea polysaccharide composite.
Description
Technical field
[0001] the present invention relates to a kind of technological method for preparing water soluble tea polysaccharide compound and caustic solubility tea polysaccharide mixture that comprehensively extracts from tealeaves, belong to field of deep processing of farm products.
Background technology
[0002] Tea Polysaccharides is the mixed polysaccharide of a kind of conjugated protein of containing in the teas such as green tea, oolong tea, black tea, black tea, white tea and yellow tea, full name is tea polysaccharide mixture (tea polysa ccharide conjugates, TPC), its polysaccharide chain part is formed by monose covalent attachment such as mouse pectinose, wood sugar, Lee's sugar, seminose, glucose, semi-lactosi, galacturonic acid and glucuronic acids; Tens seed amino acids form the peptide chain portion of its conjugated protein.The tea polysaccharide mixture hypoglycemic, strengthen immunity and the aspect such as anti-oxidant has remarkable function, be biologically active substance important in tealeaves.
The tea polysaccharide mixture has researchs and develops utility value and market outlook preferably.At present, because the content of tea polysaccharide mixture in dry tea is lower, be generally 2-4%, so in its leaching process, the main high temperature that adopts decocts, to guarantee its yield.But the tea polysaccharide of high temperature extraction has problems aspect biological activity and purity; In addition, main extraction and application water soluble tea polysaccharide still at present, its abandoned of tea grounds after extracting, but a large amount of caustic solubility tea polysaccharide mixtures is arranged in tea grounds, there is equally the effects such as hypoglycemic.As tea raw material taked before extraction to pre-treatment, make it significantly improve the yield of target product, and further extract the caustic solubility tea polysaccharide mixture in tea grounds, thus utilized to greatest extent the polysaccharide resource in tealeaves, for the deep development utilization of tea polysaccharide lays the foundation.
Summary of the invention
In order to address the above problem, the invention provides a kind of changing temperature-pressure-difference and puffing technical finesse tea raw material, the tea grounds after hot water extraction water soluble tea polysaccharide compound adopts sodium bicarbonate to extract the method for caustic solubility tea polysaccharide mixture.The technical solution used in the present invention comprises the steps:
(1) the tea raw material changing temperature-pressure-difference and puffing is processed: water is sprayed to the tea raw material in certain mass, injection flow rate is 3.0～50.0% of tea quality, this moisture tea raw material is placed in to Bulking tank, apply 0.10～0.90 MPa pressure, regulating temperature is 60～110 ℃, carry out pressure treatment, hold-time 3～50min.Then open pressure relief system, by pressure drop to 0.01 in tank～0.07 MPa, regulating temperature is 40～90 ℃, and hold-time 5～90min obtains the tea raw material that changing temperature-pressure-difference and puffing is processed.
(2) the water soluble tea polysaccharide compound extracts the tea raw material that above-mentioned changing temperature-pressure-difference and puffing is processed, add its quality 5-30 water doubly, extract 0.5-12 hour in 40-95 ℃, then use filtered through gauze, the use of filter residue caustic solubility tea polysaccharide to be extracted mixture, after filtrate is centrifugal, concentrated with the decompression rotary evaporation, concentrated solution adds papoid to carry out deproteinated, the final concentration that makes proteolytic enzyme is 0.001-0.8%, then add the decolouring of food grade hydrogen peroxide, making its final concentration is 0.05-4.0%, decoloured, it is 1000-6000 dalton ultrafiltration membrance filter that concentrated solution after decolouring adopts the membrane pore size molecular weight cut-off, trapped fluid adds 4 times of volume 95% ethanol again, standing 2-12 hour, obtain the ethanol throw out, by throw out vacuum lyophilization or spraying drying, obtain the water soluble tea polysaccharide compound.
(3) the sodium bicarbonate water that the extraction of caustic solubility tea polysaccharide mixture is 0.1-8.0% with food grade sodium bicarbonate preparation mass percent concentration.Add sodium bicarbonate water in the described filter residue of step (2), add the tea raw material quality that the quality of sodium bicarbonate water is puffing 2-25 doubly, and extract 0.5-10 hour in 30-90 ℃, then use filtered through gauze, after filtrate is centrifugal, concentrated with the decompression rotary evaporation, concentrated solution adds stomach en-, the final concentration that makes proteolytic enzyme is 0.001-0.9%, carries out deproteinated; Then add the decolouring of food grade hydrogen peroxide, making its final concentration is 0.04-4.5%, decoloured, it is 1000-6000 dalton ultrafiltration membrance filter that concentrated solution after decolouring adopts the membrane pore size molecular weight cut-off, trapped fluid adds 4 times of volume 95% ethanol again, and standing 2-12 hour obtains the ethanol throw out, by throw out vacuum lyophilization or spraying drying, obtain caustic solubility Tea Polysaccharides mixture.
Tea raw material in described step (1) is any in green tea, white tea, yellow tea, oolong tea (blue or green tea), black tea, black tea, especially usings low-grade tealeaves as the raw material that extracts the tea polysaccharide mixture.
Beneficial effect of the present invention is, the method technical process is simple, significantly improves the yield of tea polysaccharide mixture, and does not adopt virulent organic solvent, environmental protection; Fully water-soluble and caustic solubility tea polysaccharide mixture in exploitation tealeaves, with low cost, is easy to preparation of industrialization.
Claims (4)
1. the tea raw material changing temperature-pressure-difference and puffing is processed: water is sprayed to the tea raw material in certain mass, injection flow rate is 3.0～50.0% of tea quality, this moisture tea raw material is placed in to Bulking tank, apply 0.10～0.90 MPa pressure, regulating temperature is 60～110 ℃, carry out pressure treatment, hold-time 3～50min; Then open pressure relief system, by pressure drop to 0.01 in tank～0.07 MPa, regulating temperature is 40～90 ℃, and hold-time 5～90min obtains the tea raw material that changing temperature-pressure-difference and puffing is processed.
2. the water soluble tea polysaccharide compound extracts the tea raw material that above-mentioned changing temperature-pressure-difference and puffing is processed, add its quality 5-30 water doubly, extract 0.5-12 hour in 40-95 ℃, then use filtered through gauze, the use of filter residue caustic solubility tea polysaccharide to be extracted mixture, after filtrate is centrifugal, concentrated with the decompression rotary evaporation, concentrated solution adds papoid to carry out deproteinated, the final concentration that makes proteolytic enzyme is 0.001-0.8%, then add the decolouring of food grade hydrogen peroxide, making its final concentration is 0.05-4.0%, decoloured, it is 1000-6000 dalton ultrafiltration membrance filter that concentrated solution after decolouring adopts the membrane pore size molecular weight cut-off, trapped fluid adds 4 times of volume 95% ethanol again, standing 2-12 hour, obtain the ethanol throw out, by throw out vacuum lyophilization or spraying drying, obtain the water soluble tea polysaccharide compound.
3. the sodium bicarbonate water that the extraction of caustic solubility tea polysaccharide mixture is 0.1-8.0% with food grade sodium bicarbonate preparation mass percent concentration; Add sodium bicarbonate water in the described filter residue of step (2), add the tea raw material quality that the quality of sodium bicarbonate water is puffing 2-25 doubly, and extract 0.5-10 hour in 30-90 ℃, then use filtered through gauze, after filtrate is centrifugal, concentrated with the decompression rotary evaporation, concentrated solution adds stomach en-, the final concentration that makes proteolytic enzyme is 0.001-0.9%, carries out deproteinated; Then add the decolouring of food grade hydrogen peroxide, making its final concentration is 0.04-4.5%, decoloured, it is 1000-6000 dalton ultrafiltration membrance filter that concentrated solution after decolouring adopts the membrane pore size molecular weight cut-off, trapped fluid adds 4 times of volume 95% ethanol again, and standing 2-12 hour obtains the ethanol throw out, by throw out vacuum lyophilization or spraying drying, obtain caustic solubility Tea Polysaccharides mixture.
4. described by claim (1), it is characterized in that tea raw material is any in green tea, white tea, yellow tea, oolong tea (blue or green tea), black tea, black tea, especially usings low-grade tealeaves as the raw material that extracts the tea polysaccharide mixture.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103960400A (en) * | 2014-02-25 | 2014-08-06 | 中华全国供销合作总社杭州茶叶研究所 | Preparation method of instantgreen teaconcentrated juice |
| CN104621495A (en) * | 2015-02-26 | 2015-05-20 | 吴海军 | Dragon fruit peel active dietary fiber powder and preparation method thereof |
| CN104629895A (en) * | 2015-02-09 | 2015-05-20 | 吴海军 | Cucumis metuliferus fruit seed oil and preparation method thereof |
| CN105367679A (en) * | 2015-12-03 | 2016-03-02 | 镇江市丹徒区南山溪园茶叶专业合作社 | Green tea polysaccharide extraction method |
| CN107312105A (en) * | 2017-07-27 | 2017-11-03 | 福建农林大学 | A kind of Tea Polysaccharides chromic compound and its preparation and application |
| CN107397225A (en) * | 2017-09-20 | 2017-11-28 | 贵州苗贵客贸易发展有限公司 | A kind of extracting method of green-tea extract |
| CN109566929A (en) * | 2018-10-23 | 2019-04-05 | 长春大学 | The preparation method of taraxacum health beverage |
| CN110755552A (en) * | 2019-11-29 | 2020-02-07 | 贵州天诚农业科技发展有限公司 | Preparation method of herba dendrobii powder |
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| CN1379044A (en) * | 2002-03-11 | 2002-11-13 | 上海中药创新研究中心 | Purified theopolyose and its extracting process |
| CN101695326A (en) * | 2009-10-29 | 2010-04-21 | 天津农学院 | Method for preparing cold-brewing tea by puffing at variable temperature and pressure differences |
| CN101798356A (en) * | 2010-04-08 | 2010-08-11 | 晋江市恒源科技开发有限公司 | Method for separating and extracting natural tea polysaccharide |
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| CN1379044A (en) * | 2002-03-11 | 2002-11-13 | 上海中药创新研究中心 | Purified theopolyose and its extracting process |
| CN101695326A (en) * | 2009-10-29 | 2010-04-21 | 天津农学院 | Method for preparing cold-brewing tea by puffing at variable temperature and pressure differences |
| CN101798356A (en) * | 2010-04-08 | 2010-08-11 | 晋江市恒源科技开发有限公司 | Method for separating and extracting natural tea polysaccharide |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960400A (en) * | 2014-02-25 | 2014-08-06 | 中华全国供销合作总社杭州茶叶研究所 | Preparation method of instantgreen teaconcentrated juice |
| CN103960400B (en) * | 2014-02-25 | 2016-06-29 | 中华全国供销合作总社杭州茶叶研究所 | A kind of preparation method of instant type condensed juice of green tea |
| CN104629895A (en) * | 2015-02-09 | 2015-05-20 | 吴海军 | Cucumis metuliferus fruit seed oil and preparation method thereof |
| CN104621495A (en) * | 2015-02-26 | 2015-05-20 | 吴海军 | Dragon fruit peel active dietary fiber powder and preparation method thereof |
| CN104621495B (en) * | 2015-02-26 | 2017-12-26 | 广东一家亲营养科技有限公司 | A kind of dragon fruit pericarp activity food fibre powder and preparation method thereof |
| CN105367679A (en) * | 2015-12-03 | 2016-03-02 | 镇江市丹徒区南山溪园茶叶专业合作社 | Green tea polysaccharide extraction method |
| CN107312105A (en) * | 2017-07-27 | 2017-11-03 | 福建农林大学 | A kind of Tea Polysaccharides chromic compound and its preparation and application |
| CN107397225A (en) * | 2017-09-20 | 2017-11-28 | 贵州苗贵客贸易发展有限公司 | A kind of extracting method of green-tea extract |
| CN109566929A (en) * | 2018-10-23 | 2019-04-05 | 长春大学 | The preparation method of taraxacum health beverage |
| CN109566929B (en) * | 2018-10-23 | 2022-02-22 | 长春大学 | Preparation method of dandelion health-care beverage |
| CN110755552A (en) * | 2019-11-29 | 2020-02-07 | 贵州天诚农业科技发展有限公司 | Preparation method of herba dendrobii powder |
| CN110755552B (en) * | 2019-11-29 | 2021-11-23 | 贵州天诚农业科技发展有限公司 | Preparation method of herba dendrobii powder |
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| CN103450366B (en) | 2016-02-03 |
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