CN104920397A - Plant rapid growth culture solution for grafting - Google Patents
Plant rapid growth culture solution for grafting Download PDFInfo
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- CN104920397A CN104920397A CN201510328011.7A CN201510328011A CN104920397A CN 104920397 A CN104920397 A CN 104920397A CN 201510328011 A CN201510328011 A CN 201510328011A CN 104920397 A CN104920397 A CN 104920397A
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Abstract
The invention relates to a plant propagation technology, in particular to a plant rapid growth culture solution for grafting. The plant rapid growth culture solution is characterized by comprising, by weight, five to ten parts of 6-benzyl amino adenine, eight to 14 parts of naphthylacetic acid, four to nine parts of triacontanol, one to five parts of 2,4-D, five to 15 parts of vitamin A, five to 15 parts of vitamin B, one to three parts of citric acid, 0.1-0.2 part of ammonium nitrate, 0.1-0.3 part of salt, 15-25 parts of coconut juice, two to four parts of emulgator, one to three parts of corrosion remover and two to four parts of stabilizers. The grafting survival rate can be effectively improved, the seedling survival speed can be improved, and production investment can be saved.
Description
Technical field
The present invention relates to a kind of Plant breeding techniques, specifically a kind of grafting quick growth of plant culture fluid.
Background technology
Plant graft is an important Plant breeding techniques.It is widely used in horticultural production practice, and vegetables, fruit tree and the production of flowers and plants play an important role: as kept the good characteristic of kind, strengthen the resistance of scion and adaptability, promotion scion are yielded positive results, change plant type, overcome the difficulty of not easily breeding and expanding propagation coefficient etc.
People are through constantly testing, groping, in seedling fostering, adopt graft seedling growth method to instead of seed seedling-raising method gradually, the plant of grafting survival can keep the merit of scion variety, the advantageous feature of stock can be utilized again, strengthen the objects such as cold resistance, drought-resistant, disease and insect resistance, can also economic utilization propagating materials, increase nursery stock quantity to meet the needs of large-scale production.Graft seedling growth method is usually used in, in the breeding of fruit tree, forest, flowers, being also widely used in the nursery of gourd vegetables.But in grafting procedures, the various practical problem of normal generation, dead seedling after not emerging after some graftings or emerging, some wound healings are slow, growing way difference Huang Miao etc. after some healings.
Summary of the invention
The object of the invention is to propose a kind ofly to make grafting wound healing and can the plant growth culture fluid of growth promoting effects in grafting procedures.The present invention realizes above-mentioned purpose by following technical scheme:
A kind of grafting quick growth of plant culture fluid, count 6-benzyl aminoadenine 5-10 part, methyl α-naphthyl acetate 8-14 part, triacontanol 4-9 part, 2,4-D 1-5 parts, vitamin A 5-15 part, Cobastab 5-15 part, citric acid 1-3 part, ammonium nitrate 0.1-0.2 part, salt 0.1-0.3 part, Coconut Juice 15-25 part, emulsifier 2-4 part, preservative 1-3 part, stabilizing agent 2-4 part by weight.
Described emulsifier be in polyacrylamide, glyceryl monostearate and polyoxyethylene 20 sorbitan monostearate one or more.
Described preservative be in sodium sorbate, sodium lactate and sodium dehydroacetate one or more.
Described stabilizing agent is a kind of or composition of tolelofos-methyl and polyethylene glycol.
Coconut Juice is the extraction juice of the fruit coconut palm fruit of cocoanut tree.Containing a large amount of water and sugar in Coconut Juice, and the abundant mineral matter such as vitamin nitrogen, calcium, phosphorus, iron, potassium, magnesium.
6-benzyl aminoadenine is accelerated plant cell and is extended, promote the differentiation of undifferentiated tissue, cell increase getting fat, the allocation and transportation of material and accumulation, promotion cell respiration, improve anti-injury ability, suppress chlorophyllous decomposition, the activity of promotion enzyme.
Methyl α-naphthyl acetate is broad spectrum type plant growth regulator, can promote cell division and expansion.
Triacontanol promotes the growth of plant, and the accumulation increasing dry matter, the permeability improving cell membrane, increase leaf strengthen amylase, polyoxygenated enzyme, peroxidase activity and promote cell division.
2,4-D is a kind of auxin, promotes that floral organ cell grows, and accelerates plant to the absorption of mineral matter element.
Citric acid has chelation, can remove callus surface poisonous metal, can also prevent the oxidation because enzymatic and metal catalytic cause, have excellent non-oxidizability.
Nitrogen is the main component of protein, nucleic acid, phosphatide, and this three is cell membrane, cytoplasm, nuclear important component part, and they occupy special role in vital movement.Therefore, nitrogen is called as the element of life.Enzyme and many coenzyme and prothetic group are as NAD
+, NADP
+, FAD etc. formation also have nitrogen to participate in.During nitrogen stress, the biosynthesis block of the materials such as protein, nucleic acid, phosphatide, nitrogen can promote cell division, growth.
Phosphorus is the main component of nucleic acid, nucleoprotein and phosphatide, is the part of cytoplasm, cell nucleus and cell membrane.Phosphorus is that many coenzyme are as NAD
+, NADP
+deng composition, phosphorus is the composition of AMP, ADP and ATP; Phosphorus also participates in metabolism and the transport of carbohydrate; Phosphorus also plays an important role to nitrogen metabolism; Phosphorus and adipose conversion also have relation, and fat metabolism needs NADPH, ATP, CoA and NAD
+participation; Containing certain phosphate in plant cell liquor, forming buffer body is maintain Premeabilisation of cells gesture.
Potassium can be used as the activator of more than 60 kind of enzyme in cell, as pyruvate kinase, fructokinase, malate dehydrogenase, succinate dehydrogenase, amylosynthease, succinyl CoA synthetase, glutathione synthetase etc.Therefore potassium plays an important role in carbohydrate metabolism, respiration and proteometabolism.Potassium can promote the synthesis of protein, thus soluble nitrogen is reduced.
Calcium is the composition of pectate calcium in plant cell wall middle lamella, and therefore, during calcium deficiency, cell division can not be carried out maybe can not completing, and forms apocyte.Calcium ion as the bridge connect between the carboxyl of the phosphoric acid in phosphatide and protein, can have the effect of stabilising membrane structure, if sufficient for calcium, then easily forms callus.Calcium is also the activator of some enzymes, as all needed the participation of calcium ion by enzymatic reactions such as ATP hydrolase, Phospholipid hydrolase.Ca
2+be combined with CaM and form Ca
2+-CaM complex, it has messenger function in plant corpus, born of the same parents' external information can be changed into born of the same parents' internal information, in order to start, to adjust or to prevent some physiological and biochemical procedure in born of the same parents.
Magnesium is chlorophyllous composition, is again the activator of the enzyme such as RuBP carboxylase, ribulose 5-phosphate kinases; Magnesium is again the activator of the enzymes such as glucokinase, fructokinase, pyruvate kinase, acetyl CoA synthetase, isocitrate dehydrogenase, alpha Ketoglutarate dehydrase, malate synthetase, GC-syn, succinic thiokinase.
Iron is the prothetic group of many enzymes, as cytochrome, cytochrome oxidase, peroxidase and catalase etc.Can there is Fe in iron in these enzymes
3++ e
-==Fe
2+change, it breathing electron transmission in play an important role.Iron has two critical functions: one is the important composition of some enzyme and much transmission electronics albumen, and two is regulate chloroplast protein and chlorophyllous synthesis.
The described culture fluid mechanism of action: after in scion grafting to stock, on the surface of stock and scion wound, the residue due to dead cell forms the film of one deck brown, is covered with wound.Described culture fluid 6-benzyl aminoadenine, methyl α-naphthyl acetate, 2,4-D promote traumatin secretion, wound circumference cell and the vigorous division of cambial cell, and make the film breaks of brown, form callus.Vitamin A and Cobastab and Coconut Juice supply callus desired nutritional and trace element, it is impelled constantly to increase, after space between scion and stock is filled, the parenchyma cell of the callus of stock and scion just connects mutually, is coupled together by both formation layers.Callus constantly breaks up, and inwardly forms new xylem, and outwards form new phloem, and then conduit and screen casing are also communicated with each other, such anvil fringe is just combined into entity, forms a new plant.
The using method of described culture fluid: culture fluid dilutes 10 times, soaks 3-5 hour in scion callus culture fluid after dilution, and the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.
Beneficial effect of the present invention:
One, improve graft survival rate, not only can accelerate seedling speed, and can investment of production be saved;
Two, strengthen growth potential after grafting of knowing clearly, avoid branches and leaves yellow, blade is little and cluster, and grows weak, so that withered, and fruit development is abnormal, and carnification is bad, deformed fruit etc.;
Three, make anvil fringe interface grow coordination up and down, later stage compatibility is good, avoids the healing of grafting combined interface good, energy normal growth result, the serious incompatible generation of later stage performance;
Four, practical, applied widely, do not need specificly to change using method for certain plant.
Embodiment
Below by specific embodiment, the present invention is described in further detail.
Embodiment one:
6-benzyl aminoadenine 5 parts, methyl α-naphthyl acetate 8 parts, triacontanol 4 parts, 2,4-D 1 part, vitamin A 5 parts, Cobastab 5 parts, citric acid 1 part, 0.1 part, ammonium nitrate, salt 0.1 part, Coconut Juice 15 parts, emulsifier 2 parts, preservative 1 part, stabilizing agent 4 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.Accelerate about 3 days than the conventional grafting method seedling time after apple tree grafting, survival rate is to 90%, and later stage wound healing is complete, and seedling grows fine.
Embodiment two:
6-benzyl aminoadenine 5 parts, methyl α-naphthyl acetate 9 parts, triacontanol 5 parts, 2,4-D 2 parts, vitamin A 5 parts, Cobastab 5 parts, citric acid 2 parts, 0.1 part, ammonium nitrate, salt 0.2 part, Coconut Juice 15 parts, emulsifier 2 parts, preservative 3 parts, stabilizing agent 2 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.Accelerate about 2 days than the conventional grafting method seedling time after pear-tree grafting, survival rate is to 92%, and later stage wound healing is complete, and seedling grows fine.
Embodiment three:
6-benzyl aminoadenine 5 parts, methyl α-naphthyl acetate 9 parts, triacontanol 5 parts, 2,4-D 2 parts, vitamin A 5 parts, Cobastab 5 parts, citric acid 3 parts, 0.2 part, ammonium nitrate, salt 0.3 part, Coconut Juice 25 parts, emulsifier 6 parts, preservative 2 parts, stabilizing agent 3 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.Accelerate about 4 days than the conventional grafting method seedling time after peach grafting, survival rate is to 94%, and later stage wound healing is complete, and seedling grows fine.
Embodiment four:
6-benzyl aminoadenine 7 parts, methyl α-naphthyl acetate 11 parts, triacontanol 5 parts, 2,4-D 2 parts, vitamin A 5 parts, Cobastab 5 parts, citric acid 3 parts, 30.2 parts, ammonium nitrate, salt 0.13 part, Coconut Juice 20 parts, emulsifier 2 parts, preservative 2 parts, stabilizing agent 2 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.Accelerate about 5 days than the conventional grafting method seedling time after oranges and tangerines grafting, survival rate is to 95%, and later stage wound healing is complete, and seedling grows fine.
Embodiment five:
6-benzyl aminoadenine 8 parts, methyl α-naphthyl acetate 12 parts, triacontanol 6 parts, 2,4-D 2 parts, vitamin A 5 parts, Cobastab 5 parts, citric acid 2 parts, 0.12 part, ammonium nitrate, salt 0.1 part, Coconut Juice 25 parts, emulsifier 2 parts, preservative 1 part, stabilizing agent 2 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.Accelerate about 4 days than the conventional grafting method seedling time after navel orange grafting, survival rate is to 93%, and later stage wound healing is complete, and seedling grows fine.
Embodiment six:
6-benzyl aminoadenine 9 parts, methyl α-naphthyl acetate 9 parts, triacontanol 8 parts, 2,4-D 4 parts, vitamin A 8 parts, Cobastab 10 parts, citric acid 1.5 parts, 0.2 part, ammonium nitrate, salt 0.3 part, Coconut Juice 21 parts, emulsifier 2 parts, preservative 1 part, stabilizing agent 3 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.Accelerate about 2 days than the conventional grafting method seedling time after oriental cherry grafting, survival rate is to 95%, and later stage wound healing is complete, and seedling grows fine.
Embodiment seven:
6-benzyl aminoadenine 9 parts, methyl α-naphthyl acetate 14 parts, triacontanol 8 parts, 2,4-D 4 parts, vitamin A 8 parts, Cobastab 10 parts, citric acid 2.5 parts, 0.15 part, ammonium nitrate, salt 0.15 part, Coconut Juice 18 parts, emulsifier 2 parts, preservative 1 part, stabilizing agent 3 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.Accelerate about 5 days than the conventional grafting method seedling time after the Malus spectabilis grafting of the West Lake, survival rate is to 94%, and later stage wound healing is complete, and seedling grows fine.
Embodiment eight:
6-benzyl aminoadenine 10 parts, methyl α-naphthyl acetate 9 parts, triacontanol 9 parts, 2, 4-D 4 parts, vitamin A 15 parts, Cobastab 10 parts, citric acid 2.5 parts, 0.2 part, ammonium nitrate, salt 0.3 part, Coconut Juice 18 parts, emulsifier 2 parts, preservative 1 part, stabilizing agent 3 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smear in stock callus face during grafting, about 3 days are accelerated than the conventional grafting method seedling time after rose grafting, survival rate is to 97%, later stage wound healing is complete, seedling grows fine.
Embodiment nine:
6-benzyl aminoadenine 9 parts, methyl α-naphthyl acetate 9 parts, triacontanol 8 parts, 2,4-D 4 parts, vitamin A 8 parts, Cobastab 15 parts, citric acid 2 parts, 0.1 part, ammonium nitrate, salt 0.15 part, Coconut Juice 22 parts, emulsifier 5 parts, preservative 3 parts, stabilizing agent 3 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.Accelerate about 5 days than the conventional grafting method seedling time after Chinese rose grafting, survival rate is to 95%, and later stage wound healing is complete, and seedling grows fine.
Embodiment ten:
6-benzyl aminoadenine 10 parts, methyl α-naphthyl acetate 14 parts, triacontanol 9 parts, 2,4-D 5 parts, vitamin A 15 parts, Cobastab 15 parts, citric acid 3 parts, 0.2 part, ammonium nitrate, salt 0.2 part, Coconut Juice 19 parts, emulsifier 2 parts, preservative 1 part, stabilizing agent 3 parts, formulation method preparation routinely, 3-5 hour is soaked in the culture fluid of scion callus after dilution 10 times, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.Accelerate about 3 days than the conventional grafting method seedling time after camellia grafting, survival rate is to 95%, and later stage wound healing is complete, and seedling grows fine.
Although with reference to explanatory embodiment of the present invention, invention has been described here, above-described embodiment is only the present invention's preferably embodiment, embodiments of the present invention are not restricted to the described embodiments, should be appreciated that, those skilled in the art can design a lot of other amendment and embodiment, these amendments and embodiment will drop within spirit disclosed in the present application and spirit.
Claims (5)
1. a grafting quick growth of plant culture fluid, it is characterized in that comprising following component: count 6-benzyl aminoadenine 5-10 part, methyl α-naphthyl acetate 8-14 part, triacontanol 4-9 part, 2,4-D 1-5 parts, vitamin A 5-15 part, Cobastab 5-15 part, citric acid 1-3 part, ammonium nitrate 0.1-0.2 part, salt 0.1-0.3 part, Coconut Juice 15-25 part, emulsifier 2-4 part, preservative 1-3 part, stabilizing agent 2-4 part by weight.
2. a kind of grafting quick growth of plant culture fluid according to claim 1, is characterized in that: described emulsifier be in polyacrylamide, glyceryl monostearate and polyoxyethylene 20 sorbitan monostearate one or more.
3. a kind of grafting quick growth of plant culture fluid according to claim 1, is characterized in that: described preservative be in sodium sorbate, sodium lactate and sodium dehydroacetate one or more.
4. a kind of grafting quick growth of plant culture fluid according to claim 1, is characterized in that: described stabilizing agent is a kind of or composition of tolelofos-methyl and polyethylene glycol.
5. a kind of grafting quick growth of plant culture fluid according to claim 1, it is characterized in that: its using method culture fluid dilutes 10 times, 3-5 hour is soaked in scion callus culture fluid after dilution, the previous day is smeared the undiluted culture fluid of one deck in the callus face of stock in grafting, again smears in stock callus face during grafting.
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Cited By (8)
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| CN105638256A (en) * | 2016-03-25 | 2016-06-08 | 镇远县醉美果业有限公司 | Red peach seedling raising technique |
| CN106508436A (en) * | 2016-09-30 | 2017-03-22 | 道真自治县诚信农业综合开发有限公司 | Grafting and cultivation method of Taxus chinensis |
| CN107432195A (en) * | 2017-05-22 | 2017-12-05 | 滨州市沾化区冬枣研究所 | A kind of winter jujube engrafting and cultivating method |
| CN108358691A (en) * | 2018-02-08 | 2018-08-03 | 金华市飞凌生物科技有限公司 | A kind of honey peach grafting nutrient solution |
| CN108934730A (en) * | 2018-09-18 | 2018-12-07 | 广西田东泰如鲜品农副产品配送有限公司 | A kind of engrafting method improving mango survival rate |
| CN109729861A (en) * | 2019-03-25 | 2019-05-10 | 湖南省林大油茶有限公司 | A kind of oil tea engrafting method |
| CN110754281A (en) * | 2019-11-22 | 2020-02-07 | 塔里木大学 | Improved variety strong seedling cultivation method for early bearing walnut grafted seedlings |
| CN114391413A (en) * | 2022-01-27 | 2022-04-26 | 保亭智农农业发展有限责任公司 | Rapid cultivation method of durian seedlings |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638256A (en) * | 2016-03-25 | 2016-06-08 | 镇远县醉美果业有限公司 | Red peach seedling raising technique |
| CN106508436A (en) * | 2016-09-30 | 2017-03-22 | 道真自治县诚信农业综合开发有限公司 | Grafting and cultivation method of Taxus chinensis |
| CN107432195A (en) * | 2017-05-22 | 2017-12-05 | 滨州市沾化区冬枣研究所 | A kind of winter jujube engrafting and cultivating method |
| CN108358691A (en) * | 2018-02-08 | 2018-08-03 | 金华市飞凌生物科技有限公司 | A kind of honey peach grafting nutrient solution |
| CN108934730A (en) * | 2018-09-18 | 2018-12-07 | 广西田东泰如鲜品农副产品配送有限公司 | A kind of engrafting method improving mango survival rate |
| CN109729861A (en) * | 2019-03-25 | 2019-05-10 | 湖南省林大油茶有限公司 | A kind of oil tea engrafting method |
| CN110754281A (en) * | 2019-11-22 | 2020-02-07 | 塔里木大学 | Improved variety strong seedling cultivation method for early bearing walnut grafted seedlings |
| CN114391413A (en) * | 2022-01-27 | 2022-04-26 | 保亭智农农业发展有限责任公司 | Rapid cultivation method of durian seedlings |
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