CN104880449B - 一种氨氮荧光检测方法 - Google Patents
一种氨氮荧光检测方法 Download PDFInfo
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- CN104880449B CN104880449B CN201510335921.8A CN201510335921A CN104880449B CN 104880449 B CN104880449 B CN 104880449B CN 201510335921 A CN201510335921 A CN 201510335921A CN 104880449 B CN104880449 B CN 104880449B
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- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 13
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract description 54
- 238000001514 detection method Methods 0.000 claims abstract description 51
- 239000012528 membrane Substances 0.000 claims abstract description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000000725 suspension Substances 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims description 17
- 238000002791 soaking Methods 0.000 claims description 12
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 claims description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 229910021529 ammonia Inorganic materials 0.000 claims description 9
- 239000007853 buffer solution Substances 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 239000012086 standard solution Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims 1
- 229940054441 o-phthalaldehyde Drugs 0.000 description 8
- VRZJGENLTNRAIG-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]iminonaphthalen-1-one Chemical compound C1=CC(N(C)C)=CC=C1N=C1C2=CC=CC=C2C(=O)C=C1 VRZJGENLTNRAIG-UHFFFAOYSA-N 0.000 description 3
- BDOYKFSQFYNPKF-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;sodium Chemical compound [Na].[Na].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O BDOYKFSQFYNPKF-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 2
- 239000008247 solid mixture Substances 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000004005 nitrosamines Chemical class 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 230000003390 teratogenic effect Effects 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
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CN104880449A CN104880449A (zh) | 2015-09-02 |
CN104880449B true CN104880449B (zh) | 2017-11-21 |
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Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105510325A (zh) * | 2015-12-08 | 2016-04-20 | 浙江省嘉兴市农业科学研究院(所) | 农用沼液中铵态氮浓度的半定量测定方法 |
CN107091823A (zh) * | 2016-02-18 | 2017-08-25 | 常州罗盘星检测科技有限公司 | 一种低浓度氨氮荧光检测固体试剂及其检测方法 |
CN105866088B (zh) * | 2016-05-20 | 2019-01-11 | 山东省科学院海洋仪器仪表研究所 | 一种在线检测海水中氨氮含量的装置及方法 |
CN110455767A (zh) * | 2019-09-06 | 2019-11-15 | 四川轻化工大学 | 便携可视化血氨快速荧光检测装置及其制备和使用方法 |
CN110596031A (zh) * | 2019-10-14 | 2019-12-20 | 中国科学院烟台海岸带研究所 | 一种海水中氨氮定量分析装置 |
CN115308181A (zh) * | 2022-08-10 | 2022-11-08 | 中科合肥智慧农业协同创新研究院 | 一种原位可视化检测土壤铵态氮的比率荧光纸基传感器 |
CN118130194B (zh) * | 2024-03-08 | 2024-09-27 | 四川轻化工大学 | 一种顶空法荧光可视化快速检测血氨的试剂盒制备及检测方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103018219A (zh) * | 2012-12-07 | 2013-04-03 | 桂林电子科技大学 | 4,5-二甲氧基邻苯二甲醛在水中铵氮检测中的应用及测定方法 |
CN104111245A (zh) * | 2014-07-29 | 2014-10-22 | 桂林电子科技大学 | 降低邻苯二甲醛荧光法测铵氮的试剂空白的方法 |
CN104515762A (zh) * | 2015-01-19 | 2015-04-15 | 桂林电子科技大学 | 一种以滤纸为载体的测定水样中铵氮的固相荧光分析方法 |
GB2519937A (en) * | 2013-09-05 | 2015-05-13 | Kanichi Res Services Ltd | Electrochemical sensor |
CN204731161U (zh) * | 2015-06-17 | 2015-10-28 | 桂林电子科技大学 | 一种氨氮荧光检测装置 |
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2015
- 2015-06-17 CN CN201510335921.8A patent/CN104880449B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103018219A (zh) * | 2012-12-07 | 2013-04-03 | 桂林电子科技大学 | 4,5-二甲氧基邻苯二甲醛在水中铵氮检测中的应用及测定方法 |
GB2519937A (en) * | 2013-09-05 | 2015-05-13 | Kanichi Res Services Ltd | Electrochemical sensor |
CN104111245A (zh) * | 2014-07-29 | 2014-10-22 | 桂林电子科技大学 | 降低邻苯二甲醛荧光法测铵氮的试剂空白的方法 |
CN104515762A (zh) * | 2015-01-19 | 2015-04-15 | 桂林电子科技大学 | 一种以滤纸为载体的测定水样中铵氮的固相荧光分析方法 |
CN204731161U (zh) * | 2015-06-17 | 2015-10-28 | 桂林电子科技大学 | 一种氨氮荧光检测装置 |
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Address after: 541004 c413-5, 4th floor, block a, information incubation building, No. D-12, information industry park, Qixing District, Guilin City, Guangxi Zhuang Autonomous Region Patentee after: Guangxi Derun Environmental Protection Technology Co.,Ltd. Address before: 541004 c413-5, 4th floor, block a, information incubation building, No. D-12, information industry park, Qixing District, Guilin City, Guangxi Zhuang Autonomous Region Patentee before: Guilin Derun Testing Co., Ltd |
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