CN104876754A - Pleurotu comucopiae culture medium - Google Patents
Pleurotu comucopiae culture medium Download PDFInfo
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- CN104876754A CN104876754A CN201510241801.1A CN201510241801A CN104876754A CN 104876754 A CN104876754 A CN 104876754A CN 201510241801 A CN201510241801 A CN 201510241801A CN 104876754 A CN104876754 A CN 104876754A
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Abstract
The invention discloses a pleurotu comucopiae culture medium, and relates to the technical field of cultivation. The culture medium is prepared from 150-200 parts of ramulus mori, 50-100 parts of artemisia annua residue meal, 30-50 parts of silkworm excrement, 20-30 parts of a nutrient solution, 2-5 parts of gypsum and 250-300 parts of water. Compared with the prior art, the culture medium disclosed by the invention is mainly prepared from the ramulus mori, the artemisia annua residue meal and the nutrient solution; the ramulus mori, as a raw material, is easily available and is low in price; by adding the nutrient solution, the nutritive value of the cultivated pleurotu comucopiae culture medium is much higher than that cultivated with cottonseed hull.
Description
Technical field
The present invention relates to technical field of cultivation, especially a kind of pocket mushrooms substratum.
Background technology
Pocket mushrooms has another name called Pleurolus sapidus, and another name small mushroom, Ji mushroom, yellowish-white flat mushroom, belong to nutrition high, the heath food that heat is low, containing crude protein, carbohydrate, fiber, VitB1, riboflavin, nicotinic acid, calcium, iron, potassium, sodium, phosphorus.The wherein content of protein is all higher than the flat mushroom of current domestic cultivation, Twospore Mushroom, mushroom and straw mushroom; And human body eight kinds of indispensable amino acids all possess, its content accounts for more than 35% of all total amino acid contents.So often eat Pleurotus geesteranus, HUMAN HEALTH can be strengthened.According to another studying confirmation recently, some physiologically active substances that Pleurotus geesteranus contains, have the synthesis bringing out Interferon, rabbit, improve immune function of human body, have anti-cancer, anticancer effect.Pleurotus geesteranus is fatty, starch is little, is the ultimate food of diabetics and obesity patient, also has the effect reducing cholesterol.
At present, pocket mushrooms artificial culture has raw material, fermentation material and grog three kinds of forms, and because its anti-hybrid ability is strong, mycelial growth is grown fast, and China adopts raw material and fermentation material cultivation mostly.The most frequently used culture material of existing pocket mushrooms is cotton seed hulls, cotton seed hulls price rises steadily in recent years, the cost of pocket mushrooms grower is increased, and the price of pocket mushrooms lags far behind the rise with cost of material, the benefit of peasant's culturing edible fungus reduces greatly, the serious contusion plantation enthusiasm of grower; Find cheap substitute, the Sustainable development of guarantee pocket mushrooms plant husbandry.
Guangxi is one of maximum province of kind of Sang Yangcan, and ramulus mori bar has hundreds of thousands of ton every year, and the silkworm faeces of the silkworm pull-out of supporting also has several tons; According to surveying and determination, containing multiple nutrients material in ramulus mori bar, as crude protein 5.84%, xylogen 18.2%, Mierocrystalline cellulose 51.5%, ash content 1.6% etc., be suitable as very much the raw materials for production of pocket mushrooms, and the cost of ramulus mori bar is lower than cotton seed hulls, a kind of trend will be become with ramulus mori bar as the raw materials for production of pocket mushrooms; In addition, along with the development of mushroom industry, cultivated the tankage of edible mushrooms----bacterium slag also gets more and more, and defines certain pressure to environment.
Summary of the invention
It is low and cultivate the nutritious pocket mushrooms substratum of pocket mushrooms out that problem to be solved by this invention is to provide a kind of production cost.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
Be made up of the raw material of following weight part:
150 parts ~ 200 parts, ramulus mori bar, 50 ~ 100 parts, Herba Artemisiae annuae residue powder, silkworm excrement 30 ~ 50 parts, nutritive medium 20 ~ 30 parts, 2 ~ 5 parts, gypsum, 250 ~ 300 parts, water.
In technique scheme, scheme can also be more specifically: the effective constituent of described nutritive medium comprises the raw material of following weight percent: glucose 2.5%, L-glutamic acid 0.25%, ionized calcium 3%, potassium hydrogen phosphate 0.25%, magnesium sulfate 0.2%, water surplus.
Further: the preparation process of described substratum is:
The preparation process of described substratum is:
Step one, glucose, L-glutamic acid, ionized calcium, potassium hydrogen phosphate, magnesium sulfate and water are mixed after carry out high-temperature sterilization;
Step 2, ramulus mori bar is dried rear pulverizing, cross 100 mesh sieves, then mix with Herba Artemisiae annuae residue powder, add water and the stirring of step one gained nutritive medium;
Step 3, silkworm excrement to be become thoroughly decomposed;
Step 4, step 2 gains to be mixed with the silkworm excrement that becomes thoroughly decomposed of step 3 gained, then add terra alba, stir.
Further: described ionized calcium solvent contains at least one in calcium acetate, citrate of lime.
Herba Artemisiae annuae of the present invention, is the dry aerial parts of China traditional drugs artemisia Herba Artemisiae annuae, has another name called sweet wormwood, has clearing away summer heat, sterilization, desinsection, cuts the effects such as cruel, and its principle active component is antimalarial artemisinin and volatilization wet goods.For Artemisinin extraction is Herba Artemisiae annuae branch, leaf.Artemislnin content about about 1.0% in Herba Artemisiae annuae branch, leaf, industrial abstract rate about 90%, after extracting, Herba Artemisiae annuae residue fiber accounts for more than 85%, and crude protein is no less than 8%, can as fiber food; Effective component except residual Artemisinin, also containing abundant flavone component, and other water-soluble traditional Chinese medicine ingredients, can relieving summer-heat, sterilization, desinsection, cut cruel etc.In addition, Herba Artemisiae annuae is Guangxi important Chinese medicine resource, and annual Artemisinin extraction yield accounts for more than 1/3 of national Artemisinin output, this is for the enterprise of Artemisinin annual production 50 tons, nearly 6000 tons of Herba Artemisiae annuae residue, burns as coal surrogate if this part resource is only simple, really belongs to unfortunately.
The present invention compared with prior art, has following beneficial effect:
1, due in substratum of the present invention with ramulus mori bar, Herba Artemisiae annuae residue powder and nutritive medium for major ingredient, ramulus mori bar raw material is easy to get, low price; After adding nutritive medium, cultivation pocket mushrooms nutritive value is out far away higher than the pocket mushrooms nutritive value that cotton seed hulls is cultivated out.
2, the residue after Artemisinin is extracted owing to being also added with in basal culture medium of the present invention with Herba Artemisiae annuae, this residue is except residual Artemisinin, also containing abundant flavone component, and other water-soluble traditional Chinese medicine ingredients, the pocket mushrooms energy relieving summer-heat making to plant, sterilization, desinsection, cut the effects such as cruel, and the present invention makes full use of Herba Artemisiae annuae residue powder, achieves resource and uses.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.These embodiments are only illustrative, instead of limit the scope of the invention.
Embodiment 1:
The pocket mushrooms substratum of the present embodiment comprises the steps:
The preparation of A, substratum: adopt the raw material of following weight part to make substratum:
150 kilograms, ramulus mori bar, silkworm excrement 50 kilograms, Herba Artemisiae annuae residue powder double centner, 2 kilograms, gypsum, glucose 0.5 kilogram, 0.05 kilogram, L-glutamic acid, calcium acetate 6 kilograms, potassium hydrogen phosphate 0.05 kilogram, 0.04 kilogram, magnesium sulfate, 318.76 kilograms, water,
Preparation process is: step one, the water of 0.5 kilogram of glucose, 0.05 kilogram of L-glutamic acid, 6 kilograms of calcium acetates, 0.05 kilogram of potassium hydrogen phosphate, 0.04 kilo sulfuric acid magnesium and 18.76 kilograms is mixed after carry out high-temperature sterilization; Step 2,150 kilograms of ramulus mori bars are dried rear pulverizing, cross 100 mesh sieves, then mix with double centner Herba Artemisiae annuae residue powder, add 300 kg water and the stirring of step one gained nutritive medium; Step 3, silkworm excrement to be become thoroughly decomposed; Step 4, step 2 gains to be mixed with the silkworm excrement that becomes thoroughly decomposed of step 3 gained, then add 2 kilograms of terra albas, stir;
B, the substratum of step A gained is carried out building heap, pile high 1 meter, wide 1.2 meters, then will the superficial compaction of heap, get through pore every 0.5 meter;
When in C, measurement heap, 20 centimeters temperature rise to 60 DEG C, turning, turns evenly by heap with out-pile culture material, when after this turning, when material temperature is warmed up to more than 60 DEG C, is incubated 1 day again, then turning, is total to turning 3 times successively, fermentation ends;
D, spread stockpile out, adjust the potential of hydrogen of culture material, make pH value be 5.5, then pack, then by this culture medium bag as in Autoclave, in 2.5 hours, sterilizing kettle temperature is risen to 130 DEG C, be incubated 1 hour, then stop heating, more vexed 4 hours; Then, after culture medium bag being naturally cooled to 20 DEG C ~ 30 DEG C under the environment of sterilization, aseptic inoculation room is moved to;
E, under the condition of aseptic technique, be inoculated in the cooling culture material of step D gained by pocket mushrooms cultivar, more postvaccinal culture medium bag is put into culturing room's lucifuge cultivate 25 days, in culturing room, temperature is 16 ~ 28 DEG C, diurnal temperature difference is 10-12 DEG C, and relative air humidity is with 65% ~ 70%;
F, when mycelia purseful, bacterium bag is moved to mushroom room, in mushroom room, relative air humidity is 85% ~ 95%, fruiting stage mushroom room temperature is 16 ~ 18 DEG C, and strengthens ventilation, promotes that former base is grown, cultivated through 15 ~ 20 days and get final product fruiting, can gather when cap grows to 3cm.
Embodiment 2:
The pocket mushrooms substratum of the present embodiment comprises the steps:
The preparation of A, substratum: adopt the raw material of following weight part to make substratum:
200 kilograms, ramulus mori bar, silkworm excrement 30 kilograms, 50 kilograms, Herba Artemisiae annuae residue powder, 5 kilograms, gypsum, glucose 0.75 kilogram, 0.075 kilogram, L-glutamic acid, citrate of lime 9 kilograms, potassium hydrogen phosphate 0.075 kilogram, 0.06 kilogram, magnesium sulfate, 278.14 kilograms, water,
Preparation process is: step one, the water of 0.75 kilogram of glucose, 0.075 kilogram of L-glutamic acid, 9 kilograms of citrate of lime, 0.075 kilogram of potassium hydrogen phosphate, 0.06 kilo sulfuric acid magnesium and 28.14 kilograms is mixed after carry out high-temperature sterilization; Step 2,200 kilograms of ramulus mori bars are dried rear pulverizing, cross 100 mesh sieves, then mix with 50 kilograms of Herba Artemisiae annuae residue powder, add 250 kg water and the stirring of step one gained nutritive medium; Step 3, silkworm excrement to be become thoroughly decomposed; Step 4, step 2 gains to be mixed with the silkworm excrement that becomes thoroughly decomposed of step 3 gained, then add 5 kilograms of terra albas, stir;
B, the substratum of step A gained is carried out building heap, pile high 1 meter, wide 1.2 meters, then will the superficial compaction of heap, get through pore every 0.5 meter;
When in C, measurement heap, 20 centimeters temperature rise to 70 DEG C, turning, turns evenly by heap with out-pile culture material, when after this turning, when material temperature is warmed up to more than 60 DEG C, is incubated 2 days again, then turning, is total to turning 3 times successively, fermentation ends;
D, spread stockpile out, adjust the potential of hydrogen of culture material, make pH value be 6.5, then pack, then by this culture medium bag as in Autoclave, in 3.5 hours, sterilizing kettle temperature is risen to 130 DEG C, be incubated 2 hours, then stop heating, more vexed 2 hours; Then, after culture medium bag being naturally cooled to 20 DEG C ~ 30 DEG C under the environment of sterilization, aseptic inoculation room is moved to;
E, under the condition of aseptic technique, be inoculated in the cooling culture material of step D gained by pocket mushrooms cultivar, more postvaccinal culture medium bag is put into culturing room's lucifuge cultivate 30 days, in culturing room, temperature is 16 ~ 28 DEG C, diurnal temperature difference is 10-12 DEG C, and relative air humidity is with 65% ~ 70%;
F, when mycelia purseful, bacterium bag is moved to mushroom room, in mushroom room, relative air humidity is 85% ~ 95%, fruiting stage mushroom room temperature is 16 ~ 18 DEG C, and strengthens ventilation, promotes that former base is grown, cultivated through 15 ~ 20 days and get final product fruiting, can gather when cap grows to 3cm.
Claims (4)
1. a pocket mushrooms substratum, is characterized in that being made up of the raw material of following weight part:
150 parts ~ 200 parts, ramulus mori bar, 50 ~ 100 parts, Herba Artemisiae annuae residue powder, silkworm excrement 30 ~ 50 parts, nutritive medium 20 ~ 30 parts, 2 ~ 5 parts, gypsum, 250 ~ 300 parts, water.
2. pocket mushrooms substratum according to claim 1, is characterized in that: the effective constituent of described nutritive medium comprises the raw material of following weight percent: glucose 2.5%, L-glutamic acid 0.25%, ionized calcium 3%, potassium hydrogen phosphate 0.25%, magnesium sulfate 0.2%, water surplus.
3. pocket mushrooms substratum according to claim 2, is characterized in that: the preparation process of described substratum is:
Step one, glucose, L-glutamic acid, ionized calcium, potassium hydrogen phosphate, magnesium sulfate and water are mixed after carry out high-temperature sterilization;
Step 2, ramulus mori bar is dried rear pulverizing, cross 100 mesh sieves, then mix with Herba Artemisiae annuae residue powder, add water and the stirring of step one gained nutritive medium;
Step 3, silkworm excrement to be become thoroughly decomposed;
Step 4, step 2 gains to be mixed with the silkworm excrement that becomes thoroughly decomposed of step 3 gained, then add terra alba, stir.
4. pocket mushrooms substratum according to claim 2, is characterized in that: described ionized calcium solvent contains at least one in calcium acetate, citrate of lime.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107711291A (en) * | 2017-10-11 | 2018-02-23 | 诸暨马谷亲科技有限公司 | A kind of pocket mushroom culture medium for adding catkin and preparation method thereof |
| CN111149621A (en) * | 2020-01-15 | 2020-05-15 | 广东新路仕农业科技发展有限公司 | Bamboo shoot mushroom cultivation method using bamboo shoot shells to prepare culture medium |
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2015
- 2015-05-13 CN CN201510241801.1A patent/CN104876754A/en active Pending
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Cited By (2)
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|---|---|---|---|---|
| CN107711291A (en) * | 2017-10-11 | 2018-02-23 | 诸暨马谷亲科技有限公司 | A kind of pocket mushroom culture medium for adding catkin and preparation method thereof |
| CN111149621A (en) * | 2020-01-15 | 2020-05-15 | 广东新路仕农业科技发展有限公司 | Bamboo shoot mushroom cultivation method using bamboo shoot shells to prepare culture medium |
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