CN104871954A - Red Toona sinensis pollination liquid and red Toona sinensis seed cultivation production method - Google Patents
Red Toona sinensis pollination liquid and red Toona sinensis seed cultivation production method Download PDFInfo
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- CN104871954A CN104871954A CN201410072357.0A CN201410072357A CN104871954A CN 104871954 A CN104871954 A CN 104871954A CN 201410072357 A CN201410072357 A CN 201410072357A CN 104871954 A CN104871954 A CN 104871954A
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Abstract
The invention discloses a red Toona sinensis pollination liquid. The liquid comprises 1.8*10<-5>-3*10<-5>g/L of a component A and 5-20g/L of a component, wherein the component A is propionyl brassinolide; and the component B can be one or a combination containing two or more of glucan, dextran salt, konjak powder, algae and alga extract. The invention also discloses a red Toona sinensis seed cultivation production method. Red Toona sinensis seeds obtained by cultivating through the method have large and plump grains and high germination rate, and have very good economic benefits and application values.
Description
Technical field
The present invention relates to the pollination liquid of a kind of red Chinese toon and red Chinese toon breezing and producing method.
Background technology
Red Chinese toon (Toom sinensis Roem.) is Meliaceae Cedrela deciduous tree, has another name called red chinaberry.The distributed areas of red Chinese toon are extensive, have more than 2000 year, in the last few years at the cultivation history of China, nutritive value, Gen Pi and medical value really that its tender shoots is enriched, the industrial utility value of timber, and the afforestation aesthetic values etc. of plant, receive the concern of people day by day.But due to overcut, this variety breeding survival rate is low, and resource reduces day by day in addition, to sink into endangered condition, within 1996, be classified as the wild Rare plant of secondary by government.
The breeding of red Chinese toon is divided into sexual propagation and vegetative propagation.Vegetative Chinese toon very easily degenerates, poor quality and survival rate is low, usually adopts the mode of sexual propagation at present, namely with red Chinese toon seminal propagation.Red Chinese toon starts to yield positive results between 5 ~ 10 ages, and 15 ~ 40 ages are for bear seeds the phase in a large number.Under natural conditions, the red Chinese toon elite stand individual plant seed yield 0.25 ~ 1kg of life in 10 ~ 30 years, usually when mid or late October, fruit was by green flavescence brown, shows that in fruit, seed reaches the stage of ripeness, now adopts fruit, takes out seed and be conventional red Chinese toon seed.But, adopt the aforementioned naturally solid red Chinese toon seed obtained, its quality is not good, the average weight only 0.008 ~ 0.009g of single seed, full rate only 42%, by seed after Second Year sows the seed in spring, germination rate is 80% ~ 90%, seedling is thin and weak short, the sowing the highest only 1 ~ 1.2m of growing height then, the thickest only 1cm of trunk diameter.(Shanyang County, Shaanxi Province Chinese toon research institute. Chinese toon annually cultivating series technique: the biological property of I. Chinese toon and seedling-wood breeding. Chinese fruit and vegetable, 2009 (9): 49-51.).
Summary of the invention
The invention provides the pollination liquid of a kind of red Chinese toon, and adopt this pollination liquid to cultivate the method for producing the red Chinese toon seed of high-quality.
The present invention's red Chinese toon pollination liquid, containing, for example lower component in pollination liquid:
Component A 1.8 × 10
-5~ 3 × 10
-5g/L;
B component 5 ~ 20g/L;
Wherein, described component A is propionylbrassinolide; Described B component is one or more the combination in glucan, glucan hydrochlorate, konjaku flour, algae and algae extract.
Konjaku flour: refer to a kind of powder worn into by konjaku.
Propionylbrassinolide, molecular formula is: C
35h
56o
7, chemistry by name (24S)-2 α, 3 α-two propionyloxy-22R, 23R-epoxy-7-oxygen-5 α-beans steroid-6-ketone, its chemical formula following graphic shown in:
Preferably, described glucan hydrochlorate is dextran sulfate sodium or glucan potassium sulfate.
Preferably, described algae is spirulina, and described algae extract is alginic acid.
Wherein, described pollination liquid is also containing 0.6g ~ 1g/L sulphate.Preferably, described sulphate is one or more the combination in potassium sulfate, iron sulfate, zinc sulphate, magnesium sulfate, copper sulphate.
Wherein, described pollination liquid is also containing 0.2 ~ 1g/L boric acid and/or borate.Preferably, described borate is Boratex or potassium borate.
The breezing and producing method of the red Chinese toon seed of the present invention, it comprises the steps:
(1) prepare pollen liquid: at the florescence of Chinese toon, collect the red Chinese toon pollen of blooming the 1st week, with aforesaid pollination liquid with 1:(100 ~ 200) proportioning of w/v mixes, and obtains pollen liquid;
(2) artificial pollination: blooming the 2nd week, the pollen liquid of step (1) is sprayed on the pistil of red Chinese toon, each pistil spray 0.04 ~ 1ml;
(3) self-sow, picking fruit to fruit maturation, gets kind, dries.
W/v, i.e. g:ml.
In step (1), the spray method of pollen liquid is: spray 4 ~ 10 times, spray 0.01 ~ 0.1ml/ pistil at every turn.Preferably, the spraying time of pollen liquid is every morning or afternoon.
The morning: refer to 8:00 to 12:00 every day; Afternoon: refer to 13:00 to 20:00 every day.
Preferably, in April, apply fertilizer, water, the fertilizer used is biological organic fertilizer, and amount of application is 200g ~ 300g/ tree, and the consumption of water is 20 ~ 50L/ tree.
Biological organic fertilizer, refers to that a class that microorganism and organic materials (being mainly raw material with plant and animal residues and the material obtained through harmless treatment) are composited has the fertilizer of microbial manure and fertilizer effect concurrently.
Further preferably, described biological organic fertilizer is that humic fertilizer or fertility are high.
Humic fertilizer: refer to the fertilizer that plant and animal residues (as feces of livestock and poultry, agricultural crop straw etc.) rots through fermenting to obtain.
Fertility is high: be the high-efficiency composite biological bacterial manure that Beijing development corporation, Ltd. of fertility height group produces.
Preferably, 4 ~ 10 days before flowering, water, the consumption of water is l0 ~ 20L/ tree.
The present invention is adopted to pollinate liquid, the red Chinese toon seed simple grain weight using the inventive method to cultivate is large, plumpness is 85%, germination rate is 95%, seedling can reach 10cm at the average production height of sowing after 1 month, the average height (when after planting 8 months) of current growth can reach 2 meters, rhizome length is 1.5 to 2cm, the average diameter of trunk is that 1.2cm(reaches as high as 1.5cm), the average diameter of biennial trunk can reach 4 ~ 5cm, quality clearly is better than the red Chinese toon seed that naturally solid method conventional at present obtains, breeding method is easy, application prospect is good.
Obviously, according to foregoing of the present invention, according to ordinary technical knowledge and the customary means of this area, not departing under the present invention's above-mentioned basic fundamental thought prerequisite, the amendment of other various ways, replacement or change can also be made.
The embodiment of form by the following examples, is described in further detail foregoing of the present invention again.But this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following example.All technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment
Material: glucan, dextran sulfate sodium, konjaku flour, alginic acid, spirulina, propionylbrassinolide, iron sulfate, zinc sulphate, magnesium sulfate, potassium sulfate, potassium borate, Boratex, fertility are high, being is commercially available product.
The preparation of embodiment 1 the present invention red Chinese toon pollination liquid
Weigh spirulina 10g respectively, propionylbrassinolide 1.8 × 10
-5g; Be configured to 1L with water, obtain final product.
The preparation of embodiment 2 the present invention red Chinese toon pollination liquid
Weigh dextran sulfate sodium 20g respectively, propionylbrassinolide 2.5 × 10
-5g, iron sulfate or zinc sulphate 1g; Be configured to 1L with water, obtain final product.
The preparation of embodiment 3 the present invention red Chinese toon pollination liquid
Weigh Dextran 15 g respectively, propionylbrassinolide 3 × 10
-5g, Boratex 0.2g; Be configured to 1L with water, obtain final product.
The preparation of embodiment 4 the present invention red Chinese toon pollination liquid
Weigh alginic acid 12g respectively, propionylbrassinolide 1.8 × 10
-5g, magnesium sulfate or copper sulphate 0.7g, potassium borate 0.4g; Be configured to 1L with water, obtain final product.
The preparation of embodiment 5 the present invention red Chinese toon pollination liquid
Weigh konjaku flour 5g respectively, propionylbrassinolide 1.8 × 10
-5g, potassium sulfate 1g, Boratex 1g; Be configured to 1L with water, obtain final product.
The breezing and producing method of the red Chinese toon seed of embodiment 6 the present invention
(1) apply fertilizer, water: at any one day of solar calendar April, fertilising power is higher than red Chinese toon kind usage tree root, and every tree executes 200 ~ 300g, then covers thin soil (thickness is 1 ~ 2cm), water-sprinkling moisturizing, and the water that every tree is watered is 20 ~ 50L;
(2) water: 4 ~ 10 days before flowering, water to red Chinese toon kind tree, the water that every tree is watered is l0 ~ 20L;
(3) pollen liquid is prepared: annual 5 to June is the full-bloom stage of Chinese toon, about one month florescence.Red Chinese toon Post flowering, collects the red Chinese toon pollen of blooming the 1st week, by the pollination liquid of red Chinese toon pollen and embodiment 5 with 1g:(100 ~ 200) ratio of ml mixes, and obtains pollen liquid;
(4) artificial pollination: blooming the 2nd week, pollen liquid is sprayed on the pistil of red Chinese toon, altogether spray 4 ~ 10 times, spray 0.01 ~ 0.1ml/ pistil, spraying time is the morning or afternoon at every turn;
(5) gather: after fruit maturation (normally annual early December), picking fruit, takes out seed, is positioned over shady and cool place dry.
After testing, the present invention cultivates the red Chinese toon seed produced and obtain, the weight of single seed is 0.014 ~ 0.017g, and average plumpness is 85%, by seed after Second Year sows the seed in spring, average germination percentage is 95%, then (when after planting 8 months), the average height of seedling is 2 meters, and rhizome length is 1.5 to 2cm in sowing, trunk diameter average out to 1.2cm, reaches as high as 1.5m.
Embodiment 7 is pollinated the screening experiment of liquid
1, experiment grouping
Control group 1(is only containing component A): weigh glucan 10g, be configured to 1L with water, obtain pollen liquid.
Control group 2(is only containing B component): weigh propionylbrassinolide 1.8 × 10
-5g, is configured to 1L with water, obtains pollen liquid.
Control group 3(is only containing component C): weigh zinc sulphate 1g, be configured to 1L with water, obtain pollen liquid.
Control group 4(is only containing D component): weigh potassium borate 0.4g, be configured to 1L with water, obtain pollen liquid.
Experimental group 1(is containing A, B component): weigh glucan 10g, propionylbrassinolide 1.8 × 10 respectively
-5g, is configured to 1L with water, obtains pollen liquid.
Experimental group 2(is containing A, B, component C): weigh glucan 10g, propionylbrassinolide 1.8 × 10 respectively
-5g, zinc sulphate 1g, be configured to 1L with water, obtain pollen liquid.
Experimental group 3(is containing A, B, D component): weigh glucan 10g, propionylbrassinolide 1.8 × 10 respectively
-5g, potassium borate 0.4g, be configured to 1L with water, obtain pollen liquid.
Experimental group 4(is containing A, B, C, D component): weigh glucan 10g, propionylbrassinolide 1.8 × 10 respectively
-5g, zinc sulphate 1g, potassium borate 0.4g, be configured to 1L with water, obtain pollen liquid.
With aforementioned 8 kinds of pollen liquids, cultivate according to the method for embodiment 6 respectively and produce red Chinese toon seed, detect average weight and the plumpness of seed, and sow the seed in spring in Second Year, detect germination rate, height, rhizome length and the trunk diameter of seedling (when after planting 8 months) then.
2, experimental result
Experimental result is as shown in table 1:
Table 1 difference pollination liquid is on the impact of seed
Note: existing method refers to Shanyang County, Shaanxi Province Chinese toon research institute. Chinese toon annually cultivating series technique: the biological property of I. Chinese toon and seedling-wood breeding. Chinese fruit and vegetable, the seed that method disclosed in 2009 (9): 49-51. is obtained.
As shown in table 1:
The seed quality of control group 1 ~ 4 is not good, and average weight is low, plumpness is little, germination rate is low, after planting seedling short, rhizome is short and trunk is thin, suitable with the seed that existing method is obtained.
Compared with control group, the seed quality of experimental group 1 ~ 4 of the present invention significantly improves, average weight is the former 1.7 ~ 2.3 times, plumpness is the former 1.8 ~ 2.0 times, germination rate ratio former improves 7% ~ 14%, after planting the average height of seedling is the former 1.5 ~ 2.6 times, and rhizome length is the former 1.4 ~ 1.85 times, and trunk diameter is the former 1.7 ~ 2.5 times.
When being used alone glucan, propionylbrassinolide, zinc sulphate and potassium borate, obviously can not improve the quality of seed, glucan and propionylbrassinolide conbined usage then can be significantly improved the quality of seed, on the basis of glucan and propionylbrassinolide conbined usage, increase zinc sulphate and/or potassium borate again, effectively can improve the quality of seed.
Experimental result illustrates that the present invention pollinates in liquid, and the effect of Synergistic of each ingredient exerts, achieves unexpected technique effect.
To sum up, the present invention adopts specific pollen liquid to carry out artificial pollination, cultivate the seed grain obtained great, plumpness, germination rate are high, after planting, seedling growth is fast, and plant is neatly healthy and strong, obviously be better than existing breeding method and produce the red Chinese toon seed obtained, there is good economic benefit and using value.
Claims (10)
1. a red Chinese toon pollination liquid, is characterized in that: it is containing, for example lower component:
Component A 1.8 × 10
-5~ 3 × 10
-5g/L;
B component 5 ~ 20g/L;
Wherein, described component A is propionylbrassinolide; Described B component is one or more the combination in glucan, glucan hydrochlorate, konjaku flour, algae and algae extract.
2. pollination liquid according to claim 1, is characterized in that:
Described glucan hydrochlorate is dextran sulfate sodium or glucan potassium sulfate;
Described algae is spirulina, and described algae extract is alginic acid.
3. pollination liquid according to claim 1 and 2, is characterized in that: described pollination liquid is also containing 0.6g ~ 1g/L sulphate;
Preferably, described sulphate is one or more the combination in potassium sulfate, iron sulfate, zinc sulphate, magnesium sulfate, copper sulphate.
4. the pollination liquid according to claims 1 to 3 any one, is characterized in that: described pollination liquid is also containing 0.2 ~ 1g/L boric acid and/or borate;
Preferably, described borate is Boratex or potassium borate.
5. a breezing and producing method for red Chinese toon seed, is characterized in that: it comprises the steps:
(1) prepare pollen liquid: at the florescence of Chinese toon, collect the red Chinese toon pollen of blooming the 1st week, with the pollination liquid described in claim 1 ~ 7 any one with 1:(100 ~ 200) proportioning of w/v mixes, and obtains pollen liquid;
(2) artificial pollination: blooming the 2nd week, the pollen liquid of step (1) is sprayed on the pistil of red Chinese toon, each pistil spray 0.04 ~ 1ml;
(3) self-sow, picking fruit to fruit maturation, gets kind, dries.
6. breezing and producing method according to claim 5, is characterized in that: in step (1), and the spray method of pollen liquid is: spray 4 ~ 10 times, spray 0.01 ~ 0.1ml/ pistil at every turn.
7. cultivation breeding method according to claim 6, is characterized in that: the spraying time of pollen liquid is every morning or afternoon.
8. breezing and producing method according to claim 5, is characterized in that: it also comprised the steps: in April, applies fertilizer, waters, and the fertilizer used is biological organic fertilizer, and amount of application is 200g ~ 300g/ tree, and the consumption of water is 20 ~ 50L/ tree.
9. breezing and producing method according to claim 8, is characterized in that: described biological organic fertilizer is that humic fertilizer or fertility are high.
10. breezing and producing method according to claim 5, is characterized in that: it also comprises the steps: 4 ~ 10 days before flowering, waters, and the consumption of water is l0 ~ 20L/ tree.
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Cited By (2)
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CN107094565A (en) * | 2017-04-01 | 2017-08-29 | 阜南县鑫荣种养专业合作社 | A kind of North-Anhui district pear tree lists method in advance |
CN109105250A (en) * | 2018-08-06 | 2019-01-01 | 重庆市农业科学院 | A kind of efficient mung bean cross breeding method |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107094565A (en) * | 2017-04-01 | 2017-08-29 | 阜南县鑫荣种养专业合作社 | A kind of North-Anhui district pear tree lists method in advance |
CN109105250A (en) * | 2018-08-06 | 2019-01-01 | 重庆市农业科学院 | A kind of efficient mung bean cross breeding method |
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