CN104870629A - 小容积反应器中二氧化碳水平和pH的控制 - Google Patents

小容积反应器中二氧化碳水平和pH的控制 Download PDF

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Publication number
CN104870629A
CN104870629A CN201380061624.8A CN201380061624A CN104870629A CN 104870629 A CN104870629 A CN 104870629A CN 201380061624 A CN201380061624 A CN 201380061624A CN 104870629 A CN104870629 A CN 104870629A
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China
Prior art keywords
cells
liquid
gas
carbon dioxide
bioreactor system
Prior art date
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Pending
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CN201380061624.8A
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English (en)
Chinese (zh)
Inventor
希琳·吴
拉吉夫·贾加·拉姆
霍斯特·布卢姆
米开朗基罗·坎佐内里
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Sanofi SA
Massachusetts Institute of Technology
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Sanofi SA
Massachusetts Institute of Technology
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Application filed by Sanofi SA, Massachusetts Institute of Technology filed Critical Sanofi SA
Priority to CN201710541756.0A priority Critical patent/CN107267385B/zh
Publication of CN104870629A publication Critical patent/CN104870629A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Carbon And Carbon Compounds (AREA)
  • Treating Waste Gases (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN201380061624.8A 2012-10-26 2013-10-25 小容积反应器中二氧化碳水平和pH的控制 Pending CN104870629A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710541756.0A CN107267385B (zh) 2012-10-26 2013-10-25 小容积反应器中二氧化碳水平和pH的控制

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261719027P 2012-10-26 2012-10-26
US61/719,027 2012-10-26
US201361869111P 2013-08-23 2013-08-23
US61/869,111 2013-08-23
EP13306462.6 2013-10-23
EP13306462 2013-10-23
PCT/US2013/066832 WO2014066774A1 (en) 2012-10-26 2013-10-25 Control of carbon dioxide levels and ph in small volume reactors

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201710541756.0A Division CN107267385B (zh) 2012-10-26 2013-10-25 小容积反应器中二氧化碳水平和pH的控制

Publications (1)

Publication Number Publication Date
CN104870629A true CN104870629A (zh) 2015-08-26

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CN201380061624.8A Pending CN104870629A (zh) 2012-10-26 2013-10-25 小容积反应器中二氧化碳水平和pH的控制
CN201710541756.0A Expired - Fee Related CN107267385B (zh) 2012-10-26 2013-10-25 小容积反应器中二氧化碳水平和pH的控制

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Country Link
US (2) US20140127802A1 (enExample)
EP (1) EP2912157A1 (enExample)
CN (2) CN104870629A (enExample)
AU (3) AU2013334168B2 (enExample)
CA (1) CA2888076C (enExample)
HK (1) HK1213006A1 (enExample)
IN (1) IN2015DN03093A (enExample)
MX (2) MX378896B (enExample)
SG (2) SG10201803572UA (enExample)
WO (1) WO2014066774A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109517738A (zh) * 2018-12-14 2019-03-26 杭州奕安济世生物药业有限公司 一种调控生物反应器中二氧化碳含量的方法
CN114106988A (zh) * 2012-10-26 2022-03-01 麻省理工学院 化学反应器中的湿度控制
CN114989977A (zh) * 2022-06-16 2022-09-02 中山大学 一种用于多细胞相互作用及药物筛选的肿瘤类器官芯片及其制备方法
TWI885596B (zh) * 2022-11-24 2025-06-01 日商庇護日本股份有限公司 藻類增殖控制裝置

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009060489A1 (de) * 2009-12-29 2011-06-30 Uhde GmbH, 44141 Vorrichtung und Verfahren zur Regelung der Sauerstoffpermeation durch nicht-poröse Sauerstoffanionen leitende keramische Membranen und deren Verwendung
EP3036317A4 (en) 2013-08-23 2017-04-12 Massachusetts Institute of Technology Small volume bioreactors with substantially constant working volumes and associated systems and methods
US10296718B2 (en) 2014-05-30 2019-05-21 Hitachi, Ltd. Medicine production support system and medicine production support method
CN104128152B (zh) * 2014-08-12 2015-09-09 青海合杰工贸集团有限责任公司 一种调配反应观察装置
US20210146353A1 (en) * 2017-03-14 2021-05-20 BioGenium Microsystems Oy A system and a method for irradiating biological material

Citations (3)

* Cited by examiner, † Cited by third party
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US6576458B1 (en) * 2000-01-17 2003-06-10 Farzin Sarem Cell and tissue culture device with controlled culture fluid flow
WO2003093406A2 (en) * 2002-05-01 2003-11-13 Massachusetts Institute Of Technology Microfermentors for rapid screening and analysis of biochemical processes
CN1763173A (zh) * 2005-07-21 2006-04-26 高春平 造血干细胞培养装置和方法

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JP3080647B2 (ja) * 1990-10-09 2000-08-28 エーザイ株式会社 細胞培養装置
WO2005026349A1 (ja) * 2003-09-17 2005-03-24 Mitsubishi Chemical Corporation 非アミノ有機酸の製造方法
US7367550B2 (en) 2003-11-18 2008-05-06 Massachusetts Institute Of Technology Peristaltic mixing and oxygenation system
WO2005065419A2 (en) * 2003-12-29 2005-07-21 Am Biosolutions Method of culturing cells
WO2005121307A2 (en) * 2004-06-07 2005-12-22 Bioprocessor Corp. Gas control in a microreactor
US9248421B2 (en) * 2005-10-07 2016-02-02 Massachusetts Institute Of Technology Parallel integrated bioreactor device and method
US8178318B2 (en) * 2008-08-06 2012-05-15 Praxair Technology, Inc. Method for controlling pH, osmolality and dissolved carbon dioxide levels in a mammalian cell culture process to enhance cell viability and biologic product yield
PL2451936T3 (pl) * 2009-07-06 2020-02-28 F. Hoffmann-La Roche Ag Sposób hodowli komórek eukariotycznych

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6576458B1 (en) * 2000-01-17 2003-06-10 Farzin Sarem Cell and tissue culture device with controlled culture fluid flow
WO2003093406A2 (en) * 2002-05-01 2003-11-13 Massachusetts Institute Of Technology Microfermentors for rapid screening and analysis of biochemical processes
CN1763173A (zh) * 2005-07-21 2006-04-26 高春平 造血干细胞培养装置和方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114106988A (zh) * 2012-10-26 2022-03-01 麻省理工学院 化学反应器中的湿度控制
CN109517738A (zh) * 2018-12-14 2019-03-26 杭州奕安济世生物药业有限公司 一种调控生物反应器中二氧化碳含量的方法
CN114989977A (zh) * 2022-06-16 2022-09-02 中山大学 一种用于多细胞相互作用及药物筛选的肿瘤类器官芯片及其制备方法
TWI885596B (zh) * 2022-11-24 2025-06-01 日商庇護日本股份有限公司 藻類增殖控制裝置

Also Published As

Publication number Publication date
CA2888076A1 (en) 2014-05-01
WO2014066774A1 (en) 2014-05-01
MX378896B (es) 2025-03-11
AU2013334168B2 (en) 2018-10-04
HK1213006A1 (zh) 2016-06-24
IN2015DN03093A (enExample) 2015-10-02
CN107267385B (zh) 2021-03-12
AU2018286560B2 (en) 2020-07-23
CN107267385A (zh) 2017-10-20
US20170107473A1 (en) 2017-04-20
AU2013334168A1 (en) 2015-04-30
AU2020250287B2 (en) 2022-07-21
AU2018286560A1 (en) 2019-01-24
CA2888076C (en) 2023-08-08
EP2912157A1 (en) 2015-09-02
US20140127802A1 (en) 2014-05-08
MX2015005284A (es) 2015-11-16
SG11201502917TA (en) 2015-05-28
AU2020250287A1 (en) 2020-11-05
SG10201803572UA (en) 2018-06-28
MX2020014123A (es) 2021-03-25

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Application publication date: 20150826