CN104862248A - Microbial fermentation agent for fertilizers - Google Patents
Microbial fermentation agent for fertilizers Download PDFInfo
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- CN104862248A CN104862248A CN201510228366.9A CN201510228366A CN104862248A CN 104862248 A CN104862248 A CN 104862248A CN 201510228366 A CN201510228366 A CN 201510228366A CN 104862248 A CN104862248 A CN 104862248A
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- navel orange
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Fertilizers (AREA)
Abstract
The invention discloses a microbial fermentation compound fertilizer which can increase the juice yield of navel oranges. A preparation method of the microbial fermentation compound fertilizer includes the steps: (1) respectively activating pediococcus acidilactici, bacillus megatherium and aspergillus niger, and performing slant culture; (2) mixing the activated pediococcus acidilactici, the activated bacillus megatherium and the activated aspergillus niger and then placing the mixture into a triangular flask, placing the triangular flask in a shaking table, culturing the mixture for 2 days at the temperature of 28 DEG C, controlling a pH (potential of hydrogen) value to range from 6.0 to 6.5, and enabling the microbial content to reach 5-6 hundred million/ml to obtain microbial agents; (3) mixing the microbial agents with residues and bean meal formed after the navel oranges are juiced.
Description
Technical field
The invention belongs to microbial technology field, particularly a kind of preparation method improving the microorganism compound fertilizer of navel orange crushing juice rate.
Background technology
Navel orange, original name: sweet orange.Another name: yellow fruit tree, orange, navel orange, fragrant citrus, orange.Latin literary fame: Citrus sinensis (L.) Osbeck. rue order, the evergreen dungarunga of Rutaceae, sprig without hair, the short or nothing of scolus.Elliptic leaf shape or avette, full edge or cannot not have aobviously cognate; Petiole has narrow wing, and there is joint on top.Navel orange is the citrus breeding that countries in the world are cultivated unexpectedly mutually.At present, a lot of high-quality navel orange is also cultivated in China various places.Navel orange is nutritious, containing the necessary all kinds of nutrition of human body.Navel orange contains vitamin C and carotene, contributes to the formation suppressing carcinogenic substance, can also soften and protect blood vessel, stimulating circulation, and contributes to reducing cholesterol and blood fat, reduces the possibility suffered from a heart complaint, prevention gallbladder disease.Navel orange contains abundant VITAMIN, can prevent scorbutic generation.Fruit is orange-yellow and have green statin line.Being suitable for potted plant, is see leaf, sees fruit good merchantable brand.Navel orange is the citrus breeding that countries in the world are cultivated unexpectedly mutually.Now, a lot of high-quality navel orange is also cultivated in China various places.Navel orange is nutritious, containing the necessary all kinds of nutrition of human body.Navel orange contains vitamin C and carotene, contributes to the formation suppressing carcinogenic substance, can also soften and protect blood vessel, stimulating circulation, and contributes to reducing cholesterol and blood fat, reduces the possibility suffered from a heart complaint, prevention gallbladder disease.
South, Jiangxi navel orange, south, Jiangxi special product.Just, orange red bright-coloured, bright and clean attractive in appearance, edible rate reaches 85% to the large shape of south, Jiangxi navel orange fruit, and meat is tender and crisp, change slag, and the dense sweet fragrance of local flavor, containing fruit juice more than 55%.The navel orange annual production of south, Jiangxi reaches 1,000,000 tons, and Ganzhou City of country of origin Jiangxi Province has become navel orange cultivated area the first in the world, annual production third place in the world, national maximum navel orange main producing region.South, Jiangxi navel orange has been listed in one of the whole nation 11 large advantageous agricultural products, is national geography sign protection product, wins honorary titles such as " Chinese name fruits ".
Zigui navel orange is nutritious, all-ages, be shape, excellent in color eat oranges and tangerines fine work and pure natural health-care fruit raw.After measured, Zigui navel orange soluble solid content 12-15%, in every 100 milliliters of fruit juice sugary 11-13 gram, sour 0.6-0.9 gram, vitamins C 50-60 milligram, the also multiple nutritional components such as rich in proteins, inorganic salt, robust fibre, calcium.
Prior art has disclosed the microbial starter culture of a kind of conbined usage 6 kinds of microbial inoculums, and its energy and the broken foam of oranges and tangerines citrus are mixed into compound manure, can reach the effect improving crushing juice rate after being administered to south, Jiangxi navel orange.But, the method 6 kinds of microbial inoculums, preparation does not slightly want to take the trouble; In addition, only good effect is had, to Zigui navel orange poor effect to south, Jiangxi navel orange.
The present invention aims to provide a kind of microbial starter culture of raising navel orange crushing juice rate newly, and this product compared with prior art, all has the effect well improving crushing juice rate to multiple navel orange.
Summary of the invention
The object of this invention is to provide a kind of microbiobacterial agent, it can prepare compound manure, uses the crushing juice rate that this fertilizer can significantly improve multiple navel orange.
In order to realize above-mentioned object, present invention employs following technical scheme:
A preparation method for microbiobacterial agent, is characterized in that step is as follows:
(1) get and activate respectively addicted to sour micrococcus, bacillus megaterium, black-koji mould, slant culture;
(2) put into triangular flask by after each microorganism mixing after above-mentioned activation, be placed in shaking table, cultivate 2 days under the condition of 28 DEG C, control pH, at 6.0-6.5, makes bacterium number content reach 5-6 hundred million/ml, obtains microbiobacterial agent.
Described step (2) is preferably: each microbial strains presses number percent proportioning: addicted to sour micrococcus 50%-60%, bacillus megaterium 22%-25%, black-koji mould 20%-26%, and the number percent summation of above-mentioned each microbial strains is 100%.
A kind of fermentable composite fertilizer, it can improve the crushing juice rate of navel orange, and its preparation method is as follows:
(1) get and activate respectively addicted to sour micrococcus, bacillus megaterium, black-koji mould, slant culture;
(2) put into triangular flask by after each microbial strains mixing after above-mentioned activation, be placed in shaking table, cultivate 2 days under the condition of 28 DEG C, control pH, at 6.0-6.5, makes bacterium number content reach 5-6 hundred million/ml, obtains microbiobacterial agent;
(3) residue after squeezing the juice with navel orange, soya-bean cake slag carry out batch mixing.
Described step (2) is preferably: each microbial strains presses number percent proportioning: addicted to sour micrococcus 50%-60%, bacillus megaterium 22%-25%, black-koji mould 20%-26%, and the number percent summation of above-mentioned each microbial strains is 100%.
Described step (3) is preferably: wherein, the mass percent of microbiobacterial agent is 0.8%-1%.
A kind of milk-acid bacteria addicted to sour micrococcus (Pediococcus acidilactici), as raiseeing the production of additive agent for feeding and using the certification having obtained Chinese and EU, and can be applicable to many countries and regions such as the U.S. and Brazil, its security has obtained the certification of international main authoritative institution (as U.S. FDA, European Union SCAN etc.), is the novel fodder additive being selected to improve the green of animal digestive tract microecosystem, safety, environmental protection.
But also do not report and find to may be used for fertilizer addicted to sour micrococcus, especially improve in the fertilizer of navel orange crushing juice rate.
Usefulness of the present invention is:
1. this product compared with prior art, and only use 3 kinds of bacterial strains, prepare more convenient, and fermentation time is short, preparation cost is low.
2. microbiobacterial agent of the present invention may be used for the fertilizer preparing multiple navel orange, uses wide.
3. can improve the crushing juice rate of navel orange very significantly.
Embodiment
Microbial inoculum of the present invention is business and buys acquisition.
embodiment 1:
A kind of fermentable composite fertilizer, it can improve the crushing juice rate of navel orange, and its preparation method is as follows:
Get and activate respectively addicted to sour micrococcus, bacillus megaterium, black-koji mould, slant culture;
Each microbial strains after above-mentioned activation is pressed number percent proportioning: addicted to sour micrococcus 55%, huge gemma bar 25%, black-koji mould 20%, the number percent summation of above-mentioned each microbial strains is 100%, triangular flask is put into after mixing, be placed in shaking table, cultivate 2 days under the condition of 28 DEG C, control pH, 6.5, makes bacterium number content reach 500,000,000/ml, obtains microbiobacterial agent;
Residue after squeezing the juice with navel orange, soya-bean cake slag carry out batch mixing (ratio is 7:3), and wherein, the mass percent of microbiobacterial agent is 1%, ferments 72 hours.
embodiment 2:
A kind of fermentable composite fertilizer, it can improve the crushing juice rate of navel orange, and its preparation method is as follows:
Get and activate respectively addicted to sour micrococcus, bacillus megaterium, black-koji mould, slant culture;
Each microbial strains after above-mentioned activation is pressed number percent proportioning: addicted to sour micrococcus 52%, bacillus megaterium 22%, black-koji mould 26%, the number percent summation of above-mentioned each microbial strains is 100%, triangular flask is put into after mixing, be placed in shaking table, cultivate 2 days under the condition of 28 DEG C, control pH, 6.0, makes bacterium number content reach 600,000,000/ml, obtains microbiobacterial agent;
Residue after squeezing the juice with navel orange, soya-bean cake slag carry out batch mixing (ratio is 7:3), and wherein, the mass percent of microbiobacterial agent is 0.8%, ferments 72 hours.
embodiment 3:
A kind of fermentable composite fertilizer, it can improve the crushing juice rate of navel orange, and its preparation method is as follows:
Get and activate respectively addicted to sour micrococcus, bacillus megaterium, black-koji mould, slant culture;
Each microbial strains after above-mentioned activation is pressed number percent proportioning: addicted to sour micrococcus 53%, bacillus megaterium 24%, black-koji mould 23%, the number percent summation of above-mentioned each microbial strains is 100%, triangular flask is put into after mixing, be placed in shaking table, cultivate 2 days under the condition of 28 DEG C, control pH, 6.2, makes bacterium number content reach 500,000,000/ml, obtains microbiobacterial agent;
Residue after squeezing the juice with navel orange, soya-bean cake slag carry out batch mixing (ratio is 7:3), and wherein, the mass percent of microbiobacterial agent is 0.9%, ferments 72 hours.
embodiment 4:
A kind of fermentable composite fertilizer, it can improve the crushing juice rate of navel orange, and its preparation method is as follows:
Get and activate respectively addicted to sour micrococcus, bacillus megaterium, black-koji mould, slant culture;
Each microbial strains after above-mentioned activation is pressed number percent proportioning: addicted to sour micrococcus 50%, bacillus megaterium 25%, black-koji mould 25%, the number percent summation of above-mentioned each microbial strains is 100%, triangular flask is put into after mixing, be placed in shaking table, cultivate 2 days under the condition of 28 DEG C, control pH, 6.4, makes bacterium number content reach 600,000,000/ml, obtains microbiobacterial agent;
Residue after squeezing the juice with navel orange, soya-bean cake slag carry out batch mixing (ratio is 7:3), and wherein, the mass percent of microbiobacterial agent is 1%, ferments 72 hours.
embodiment 5:
A kind of fermentable composite fertilizer, it can improve the crushing juice rate of navel orange, and its preparation method is as follows:
Get and activate respectively addicted to sour micrococcus, bacillus megaterium, black-koji mould, slant culture;
Each microbial strains after above-mentioned activation is pressed number percent proportioning: addicted to sour micrococcus 60%, bacillus megaterium 20%, black-koji mould 20%, the number percent summation of above-mentioned each microbial strains is 100%, triangular flask is put into after mixing, be placed in shaking table, cultivate 2 days under the condition of 28 DEG C, control pH, 6.3, makes bacterium number content reach 500,000,000/ml, obtains microbiobacterial agent;
Residue after squeezing the juice with navel orange, soya-bean cake slag carry out batch mixing (ratio is 7:3), and wherein, the mass percent of microbiobacterial agent is 0.8%, ferments 72 hours.
embodiment 6 effect is tested and assessed:
The micro organism composite fertilizer obtained by embodiment 1-5, is applied to navel orange tree as fertilizer.
The common fertilizer be only made up of stalk, soya-bean cake slag, chicken manure is applied to as a control group at identical conditions respectively in navel orange of the same area test canopy.Test-results is as follows:
Test-results shows, the fertilizer that microbiobacterial agent of the present invention prepares, and effectively can improve output and the crushing juice rate of various navel orange.
In addition, after also finding to use this fertilizer, relative comparison group, the sickness rate of anthrax, dark mildew have dropped 50%-60% and 62%-65% respectively.
The above; be only the present invention's preferably embodiment; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.
Claims (5)
1. a preparation method for microbiobacterial agent, is characterized in that step is as follows:
(1) get and activate respectively addicted to sour micrococcus, bacillus megaterium, black-koji mould, slant culture;
(2) put into triangular flask by after each microorganism mixing after above-mentioned activation, be placed in shaking table, cultivate 2 days under the condition of 28 DEG C, control pH, at 6.0-6.5, makes bacterium number content reach 5-6 hundred million/ml, obtains microbiobacterial agent.
2. the preparation method of microbiobacterial agent according to claim 1, it is characterized in that: described step (2) is: each microbial strains is by number percent proportioning: addicted to sour micrococcus 50%-60%, bacillus megaterium 22%-25%, black-koji mould 20%-26%, the number percent summation of above-mentioned each microbial strains is 100%.
3. a fermentable composite fertilizer, it can improve the crushing juice rate of navel orange, and its preparation method is as follows:
(1) get and activate respectively addicted to sour micrococcus, bacillus megaterium, black-koji mould, slant culture;
(2) put into triangular flask by after each microbial strains mixing after above-mentioned activation, be placed in shaking table, cultivate 2 days under the condition of 28 DEG C, control pH, at 6.0-6.5, makes bacterium number content reach 5-6 hundred million/ml, obtains microbiobacterial agent;
(3) residue after squeezing the juice with navel orange, soya-bean cake slag carry out batch mixing.
4. fermentable composite fertilizer according to claim 3, step described in it (2) is: each microbial strains is by number percent proportioning: addicted to sour micrococcus 50%-60%, bacillus megaterium 22%-25%, black-koji mould 20%-26%, and the number percent summation of above-mentioned each microbial strains is 100%.
5. fermentable composite fertilizer according to claim 4, described step (3) is preferably: wherein, the mass percent of microbiobacterial agent is 0.8%-1%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510228366.9A CN104862248A (en) | 2015-05-07 | 2015-05-07 | Microbial fermentation agent for fertilizers |
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| CN201510228366.9A CN104862248A (en) | 2015-05-07 | 2015-05-07 | Microbial fermentation agent for fertilizers |
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| CN104862248A true CN104862248A (en) | 2015-08-26 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190899A (en) * | 2016-07-12 | 2016-12-07 | 德清龙溪农业科技有限公司 | A kind of microbe leaven for Fuji apple organic fertilizer |
Citations (5)
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| CN101857510A (en) * | 2010-06-22 | 2010-10-13 | 张才建 | Special organic composite fertilizer of citrus residues of citrus and preparation method thereof |
| US20110287157A1 (en) * | 2004-05-25 | 2011-11-24 | Watson James B | Live bacteria product |
| CN103819239A (en) * | 2014-03-04 | 2014-05-28 | 重庆圣沛农业科技有限公司 | Orange peel dregs biologic fermentation method |
| CN103834604A (en) * | 2014-04-02 | 2014-06-04 | 赣州天绿生化科技有限公司 | Multi-strain microorganism leavening agent and preparation method thereof |
-
2015
- 2015-05-07 CN CN201510228366.9A patent/CN104862248A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20110287157A1 (en) * | 2004-05-25 | 2011-11-24 | Watson James B | Live bacteria product |
| CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial inoculum |
| CN101857510A (en) * | 2010-06-22 | 2010-10-13 | 张才建 | Special organic composite fertilizer of citrus residues of citrus and preparation method thereof |
| CN103819239A (en) * | 2014-03-04 | 2014-05-28 | 重庆圣沛农业科技有限公司 | Orange peel dregs biologic fermentation method |
| CN103834604A (en) * | 2014-04-02 | 2014-06-04 | 赣州天绿生化科技有限公司 | Multi-strain microorganism leavening agent and preparation method thereof |
Non-Patent Citations (2)
| Title |
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| NIHARIKA SOOD ET AL.: "EVALUATION OF PHARMACOLOGICAL ACTIVITIES OF PECTIN EXTRACTED FROM APPLE AND CITRUS POMACE", 《BIOLIFE》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190899A (en) * | 2016-07-12 | 2016-12-07 | 德清龙溪农业科技有限公司 | A kind of microbe leaven for Fuji apple organic fertilizer |
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Application publication date: 20150826 |
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