CN104856057A - Composition promoting sleep, as well as preparation method, preparation and application of composition - Google Patents
Composition promoting sleep, as well as preparation method, preparation and application of composition Download PDFInfo
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- 241000180649 Panax notoginseng Species 0.000 claims abstract description 48
- 235000003143 Panax notoginseng Nutrition 0.000 claims abstract description 48
- 239000000843 powder Substances 0.000 claims abstract description 39
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a composition promoting sleep, as well as a preparation method, a preparation and an application of the composition. The composition promoting the sleep comprises gastrodia elata powder and an stem and leaf extract of panax notoginseng at a weight ratio of (8-12):1. The preparation method comprises the step of mixing the prepared gastrodia elata powder and the stem and leaf extract of the panax notoginseng according to a proportion in a formula. The preparation is powder, a tablet, a capsule, a granule, a drop pill or a honeyed pill prepared by adding a pharmaceutically acceptable auxiliary material into the composition promoting the sleeping. The application is to apply the composition promoting the sleep to prepare a food additive, food and health food promoting the sleep. The composition has an effect of promoting the sleep, and can improve a nerve function and relax anxiety. The composition can serve as a raw material of a health product promoting the sleep, and as the food additive.
Description
Technical field
The invention belongs to health-oriented products deep process technology field, be specifically related to a kind of promote to sleep composition and method of making the same, preparation and application.
Background technology
Insomnia is common illness, has a strong impact on the life of people, work and physical and mental health.Since this century, along with rhythm of life is accelerated, operating pressure increases, and psychological burden increases the weight of, and the quantity of insomniac constantly rises.The research of WHO shows, the whole world about has 27% crowd to suffer puzzlement (Taylor DJ, the Lichstein KL of sleep disorder, Durrence HH., Insomnia as a health risk factor.Behav Sleep Med, 2003,1 (4): 227-247).The incidence of disease of China's serious insomnia disease is 9.38%(Xu Zhi roc, Lu Wenjun, Li Honghua etc., the research of insomnia and treatment.China's clinical rehabilitation, 2006,10 (22): 151-153).Along with the increase that people pay close attention to insomnia, in recent years, arise at the historic moment for the medicine of improving water flood and health products.But also can not meet the need of market far away, therefore, the health-oriented products developing a kind of improving water flood are very important.
Summary of the invention
The first object of the present invention is to provide a kind of composition promoting to sleep; Second object is to provide the described preparation method promoting the composition of sleep; 3rd object is to provide the described preparation promoting the composition of sleep; 4th object is to provide the described application promoting the composition of sleep.
The first object of the present invention is achieved in that the composition of described promotion sleep comprises rhizoma Gastrodiae powder and Panax Notoginseng Folium Saponins, and described rhizoma Gastrodiae powder and Panax Notoginseng Folium Saponins weight ratio are 8 ~ 12:1.
The second object of the present invention is achieved in that and the rhizoma Gastrodiae powder prepared and Panax Notoginseng Folium Saponins is mixed to get by formula rate.
The third object of the present invention is achieved in that adding the auxiliary material pharmaceutically accepted in the composition of described promotion sleep is prepared into powder, tablet, capsule, granule, pill or honey pill agent.
The fourth object of the present invention is achieved in that and describedly promotes that the composition of sleep promotes the health-oriented products of sleep, food additives, application in health food in preparation.
Rhizoma Gastrodiae (
gastrodia elatabl .) also known as rhizoma gastrodiae, DINGFENGCAO, solely broadcast sesame etc., being perennial heterotrophism parasitic plant, is the traditional Chinese herbal medicines of China.Medicinal rhizome, has the effects such as suppressing hyperactive liver for calming endogenous wind, relieving convulsion pain relieving, tranquilizing and allaying excitement.Shennong's Herbal before two thousand years is just on the books.Modern study finds, rhizoma Gastrodiae have calmness, anticonvulsion, promote sleep, anti-inflammatory, enhancing immunologic function and the pharmacological action such as to delay senility (Yang Shilin, Lan Jin, Xu Jintang. the progress of rhizoma Gastrodiae. Chinese herbal medicine, 2000,31 (1): 66-69).Be used for the treatment of clinically to have a headache, combined external head injuries that dizzy, sleep-disorder is cardinal symptom, also be used for the treatment of the neurogenic disease (Du Guiyou such as neurasthenia, depressive neurosis and Effects of Senile Vascular Dementia Treated, Chen Kai, Zhou Wenquan, Deng. Clinical Effect of Tianma cuzhi Granules on Senile Vascular Dementia. Chinese medicine will, 1998,23 (11): 695).The active component of rhizoma Gastrodiae is Gastrodin.Gastrodin have improving water flood effect (Huang Junhua, Wang Guilian. the Primary Study of Gastrodia Injection and gastrodin pharmacological action.Chinese Academy of Medical Sciences's journal, 1985,7 (5): 399; Li Yongli, Yu Bin.The experimental study of Rhizoma Gastrodiae powder sheet improving water flood. experimental study, 2009,6 (16): 39-41; Yu Ping, Xu Dezhou.The experimental study of Gastrodin goods improving water flood effect, modern preventive medicine, 2007,34 (16): 3056-3057).The content of Gastrodin affects the quality of rhizoma Gastrodiae.The preliminary working method of rhizoma Gastrodiae is then the key factor affecting gastrodin content.
Pseudo-ginseng (
panax notoginseng(Burk.) F.H.Chen) be important Chinese medicine, main product is in Yunnan.Modern study shows, saponin of Radix Notoginseng leaf has tranquilizing and allaying excitement and antidotal effect (Lei Weiya etc.: the analgesia of sanchi leaf Total saponin is by the inhibitory action to central nervous system; Bulletin of Chinese materia medica, 1984,9 (3): 38).Seven leaf mind tranquilizing tablet (Xiang Shengqun, Xiang Mingli of the eighties exploitation in last century; The clinical practice of Qiyeshen' and tablet, modern medicine health, 2005, (24): 3453-3454), be namely take sanchi leaf as raw material.Sanchi leaf is rich in saponin constituent.Saponin of Radix Notoginseng leaf has effect (the Hui Xiang of anti-melancholy, Yingxue Liu, Baibing Zhang, et al.The antidepressant effects and mechanism of action of total saponins from the caudexes and leaves of Panax notoginseng in animal models of depression [J]. Phytomedicine, 2011:731-738).Wherein, ginsenoside Rb3 is main active (Li Haizhou, Zhang Yingjun, Yang Chongren of sanchi leaf; The further research of sanchi leaf chemical composition. research and development of natural products. 2006,18:549-554.).
Advantage of the present invention:
1, the invention provides a kind of processing method of rhizoma Gastrodiae powder, the method is steamed and the technique such as ultramicro grinding by normalized, obtains standardized rhizoma Gastrodiae powder, and measures the content of active material Gastrodin with high-pressure liquid chromatography.
2, the invention provides a kind of preparation method of notoginseng haulm standard extract, the method is by fining agent clarification, the alcohol precipitation removal of impurity, macroporous absorbent resin resin and ion exchange resin column chromatographic purifying, and the normalization process such as spraying dry, prepare notoginseng haulm standard extract.The saponin content of this standard extract is detected as above 90% through ultraviolet spectroscopy.Wherein, the content high pressure lipuid chromatography (HPLC) of active component ginsenoside Rb3 is detected as more than 20%.Meanwhile, with high-pressure liquid chromatography, establish the finger-print of notoginseng haulm standard extract.
What 3, obtain by preparation method provided by the invention is raw material with rhizoma Gastrodiae powder and notoginseng haulm standard extract natural plant composition GNF901 has the effect promoting sleep, and can improve nervous function, relaxes anxiety.Can be used as the raw material of the health-oriented products of food additives or promotion sleep.
Accompanying drawing explanation
Fig. 1 is that fresh gastrodia elata 100 DEG C steams and affects schematic diagram to gastrodin content;
Fig. 2 is that fresh gastrodia elata 110 DEG C steams and affects schematic diagram to gastrodin content;
Fig. 3 is that fresh gastrodia elata 120 DEG C steams and affects schematic diagram to gastrodin content;
Fig. 4 is that baking temperature affects schematic diagram to gastrodin content;
Fig. 5 is the high pressure liquid chromatography finger-print (collection of illustrative plates is in order from top to bottom) of the Panax Notoginseng Folium Saponins that embodiment 4,5,6 prepares.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is further illustrated, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or replacement, all belong to protection scope of the present invention.
The composition promoting sleep of the present invention, comprise rhizoma Gastrodiae powder and Panax Notoginseng Folium Saponins, described rhizoma Gastrodiae powder and Panax Notoginseng Folium Saponins weight ratio are 8 ~ 12:1.
Rhizoma Gastrodiae powder be with orchid rhizoma Gastrodiae (
gastrodia elatabl.) fresh tuber is raw material, and through steaming, dry and pulverising step prepares, and specifically comprises the following steps:
A, to steam: select fresh gastrodia elata, steam 5 ~ 10h under being placed in temperature 100 ~ 120 DEG C of steam for subsequent use;
B, drying: the rhizoma Gastrodiae after steaming is carried out being dried to water content 8 ~ 10% below temperature 60 C;
C, pulverizing: dried rhizoma Gastrodiae is carried out ultramicro grinding and cross 300 orders and obtain object rhizoma Gastrodiae powder with top sieve, be namely less than the rhizoma Gastrodiae powder of 48 μm.
Described fresh gastrodia elata is through to be selected, and meat is full, size evenly, the fresh gastrodia elata of milky, transparent, short fertilizer.Include but not limited to the rhizoma Gastrodiae of the real estates such as Yunnan, Guizhou, Sichuan, Shaanxi.Include but not limited to the kinds such as gastrodia elata f. glauca, red rhizoma Gastrodiae and green rhizoma Gastrodiae.
Described rhizoma Gastrodiae powder, the content of its active material Gastrodin is more than 1%.
Drying described in step B is the one in low-temperature aeration drying, heated-air drying, heat drying or freeze drying.
Described Panax Notoginseng Folium Saponins is that raw material prepares through raw material preparation, extraction, post-processing step with notoginseng haulm, specifically comprises the following steps:
A, raw material prepare: be equal to or higher than 7:3 by the weight ratio of sanchi leaf and pseudo-ginseng stem and carry out weighing preparation and obtain raw material notoginseng haulm;
B, extraction: get raw material notoginseng haulm, adding volume ratio is 4 ~ 5 times, concentration be 50 ~ 80% ethanol extract, filter, the medicinal extract a that filtrate is condensed into relative density 1.1 ~ 1.3 is for subsequent use;
C, post processing:
1) in medicinal extract a, adding water to relative density is 0.9 ~ 1.08, is heated to 70 ~ 80 DEG C, and adding with medicinal extract a weight ratio is the fining agent of 50:1-80:1, stir, place and naturally cool to room temperature, centrifugal, it is 0.9 ~ 1.08 obtain medicinal extract b that clarified solution after centrifugal is concentrated into relative density
2) in medicinal extract b, ethanolic solution is added, solution ethanol concentration is regulated to be 40 ~ 60%, stir, centrifugal, add the water of volume ratio 10:1-50:1 in the clarified solution after centrifugal, obtain solution a, by macroporous adsorption resin chromatography post, in succession carry out wash-out with solution a volume ratio 5-10 water, the ethanolic solution of solution a volume ratio 5:10 times of volumetric concentration 30% and the ethanolic solution of solution a volume ratio 5:10 times of volumetric concentration 80% doubly respectively, the eluent of collected volume concentration 80% ethanolic solution obtains eluent a
3) eluent a is again through ion exchange resin column column chromatography, carries out wash-out with eluent a volume ratio 5:10 7% ethanol doubly, collects eluent and obtains eluent b, by eluent b through concentrating, obtain object Panax Notoginseng Folium Saponins after drying.
The saponin content of described Panax Notoginseng Folium Saponins is not less than 90%, and wherein ginsenoside Rb3 content is more than or equal to 20%.
Notoginseng haulm described in step A, the weight ratio of its sanchi leaf and pseudo-ginseng stem must be equal to or higher than 7:3.
The one be extracted as in refluxing extraction, ultrasonic wave extraction, Microwave Extraction, counter-flow extraction described in step A.
Fining agent described in step B is ZTC 1+1, gelatin or shitosan.
The filler of the macroporous adsorption resin chromatography post described in step B is the one in D-101, DA-201, D1400, CAD-40, ADS-5, HPD-500, DM130, XAD-2, XAD-4, Diaion or AB-8 model macroporous absorbent resin; The filler that described resinbed analyses post is the one in D941, D280, D296, D900, D396, D390, D311 or D314 model anion exchange resin.
The preparation method promoting the composition of sleep of the present invention is mixed to get by formula rate the rhizoma Gastrodiae powder prepared and Panax Notoginseng Folium Saponins.
The preparation of the composition of promotion sleep of the present invention is add the auxiliary material pharmaceutically accepted in the composition of described promotion sleep to be prepared into powder, tablet, capsule, granule, pill or honey pill agent.
The composition being applied as described promotion sleep of the present invention promotes the application in the health-oriented products of sleep, food additives, health food in preparation.
Concrete preparation section of the present invention comprises: preferably fresh gastrodia elata, by only making, shredding, steam, drying, the operation such as pulverizing, obtain the rhizoma Gastrodiae powder being rich in Gastrodin; Air-dry notoginseng haulm, by extraction, clarification, chromatography, the operation such as concentrated, dry, obtains the position of being rich in active saponin; The two mixes by a certain percentage, obtains having the composition promoting sleep function.
Concrete procedure of processing is as follows:
1, rhizoma Gastrodiae powder process: selection matter of fine quality, size is even, meat is full, milky white transparent fresh gastrodia elata is raw material, with circulating water cleaning, being cut into thickness is 0.1-1.0cm, length is fritter or the small pieces of 0.5-2cm, put into high-pressure boiler, under the saturated vapor of 100-120 DEG C, steam 5-10 hour, the technical parameter steamed, obtained (Fig. 1-3) by Optimum Experiment, in 50-80 DEG C of drying, drying condition is obtained (Fig. 4) by Optimum Experiment, ultramicro grinding, obtains rhizoma Gastrodiae powder.Gastrodin content assay method is set up with high-pressure liquid chromatography.
2, the preparation method of notoginseng haulm standard extract: the pseudo-ginseng stem of 3:7 and leaf by weight proportion, uses hydrous ethanol heating and refluxing extraction, filters, concentrated, concentrate is diluted with water, and drops into natural clarifying agent after heating, stir, room temperature is placed, centrifugal, is 1.08 to density, add ethanol, make concentration be 45%, stir, place, centrifugal, the settled solution obtained, with purification with macroreticular resin, 80% ethanol eluate, analyse through resinbed, concentrated, spraying dry, obtains notoginseng haulm standard extract.The content ultraviolet spectroscopy of its saponin(e detects, and the content of active component ginseng sapoglycoside Rg 3 detects with high pressure lipuid chromatography (HPLC).Meanwhile, the finger-print of standard extract is established with high-pressure liquid chromatography.
3, gastrodia elata submicron powder body and notoginseng haulm standard extract mix by a certain percentage, namely obtain composition.
Embodiment 1---prepare rhizoma Gastrodiae powder
Selected fresh gastrodia elata 10kg, circulating water is cleaned, chopping, insert high-pressure sterilizing pot, 100 DEG C steam 10 hours, put into hot air circulation drying oven, 60 DEG C of heated-air dryings, pulverize, by 12-18 order, ultramicro grinding, obtains sepia rhizoma Gastrodiae powder 2.6 kg, high pressure liquid chromatography quantitative analysis, gastrodin content is 1.06%.
Embodiment 2---prepare rhizoma Gastrodiae powder
Selected fresh gastrodia elata 10kg, circulating water is cleaned, and is cut into sheet, inserts high-pressure sterilizing pot, 110 DEG C steam 9 hours, put into freeze drying box, low temperature drying, pulverize, ultramicro grinding, obtains rhizoma Gastrodiae powder 3.2kg, and through high pressure liquid chromatography quantitative analysis, gastrodin content is 1.03%.
Embodiment 3---prepare rhizoma Gastrodiae powder
Selected fresh gastrodia elata 10kg, cleans with circulating water, chopping, insert in high-pressure sterilizing pot, 120 DEG C steam 7 hours, put into low-temperature aeration drying box, 50 DEG C of dryings, pulverize, by 60 orders, ultramicro grinding, obtains rhizoma Gastrodiae powder 2.5kg, through high pressure liquid chromatography quantitative analysis, gastrodin content is 1.08%.
Embodiment 4---prepare Panax Notoginseng Folium Saponins
Notoginseng haulm 30kg, add 70% ethanol of 4-5 times amount, circumfluence distillation 2 times, each 3 hours, filter, merging filtrate, being condensed into relative density is 1.2(60 DEG C)) medicinal extract, add suitable quantity of water, density is made to be 1.08(60 DEG C), add temperature to 75 DEG C, add natural clarifying agent, stir, be placed to room temperature, centrifugal, it is 1.08(60 DEG C that clarified solution is concentrated into density), add ethanol, be 50% to solution ethanol concentration, stir, centrifugal, clarified solution is diluted with water, by DM130 type macroporous adsorbent resin column chromatography, in succession use water, 30% ethanol and 80% ethanol elution, collect 80% ethanol eluate, with D941 type ion exchange resin column chromatographic purifying, eluent concentrates, spraying dry, obtain notoginseng haulm standard extract 1.57kg altogether.Detect with ultraviolet spectroscopy, total saponin content is 95.2%; Detect with high pressure lipuid chromatography (HPLC), ginsenoside Rb3 content is 24.8%, and finger-print is as Fig. 5.
Embodiment 5---prepare Panax Notoginseng Folium Saponins
Notoginseng haulm 15kg, adds 70% ethanol of 4-5 times amount, heating and refluxing extraction, filter, filtrate simmer down to relative density 1.2(60 DEG C) medicinal extract.Medicinal extract adds water and makes density be 1.10(60 DEG C), heat to 78 DEG C, add fining agent, stir, place, centrifugal, it is 1.08(60 DEG C that clarified solution is concentrated into density), adding ethanol makes concentration be 60%, stir, place, centrifugal, clarified solution is diluted with water, by D101 type macroporous adsorbent resin column chromatography, with water, 30% ethanol and 80% ethanol wash-out in succession, collect 80% ethanol eluate, through D941 type ion exchange resin column chromatographic purifying, eluent concentrates, spraying dry, obtain notoginseng haulm standard extract 767g altogether, detect with ultraviolet spectroscopy, total saponin content is 92.6%, detect with high pressure lipuid chromatography (HPLC), ginsenoside Rb3 content is 22.5%, and finger-print is as Fig. 5.
Embodiment 6---prepare Panax Notoginseng Folium Saponins
Notoginseng haulm 20kg, adds 70% ethanol of 4-5 times amount, circumfluence distillation 2 times, each 3 hours, filter, merging filtrate, is condensed into relative density 1.2(60 DEG C) medicinal extract.Add water as density is 1.08(60 DEG C) the aqueous solution, be heated to 78 DEG C, add fining agent, stir, place, centrifugal, it is 1.08(60 DEG C that settled solution is concentrated into density), adding ethanol to concentration is 45% ethanolic solution, stir, place, centrifugal, settled solution concentrates, be diluted with water, by DM 130 type macroporous adsorbent resin column chromatography, in succession use water, 30% ethanol and 85% ethanol elution, collect 85% ethanol eluate, with D941 type ion exchange resin column chromatographic purifying, eluent concentrates, spraying dry, obtain notoginseng haulm standard extract, 1.07kg altogether, detect with ultraviolet spectroscopy, total saponin content is 96.6%, detect with high pressure lipuid chromatography (HPLC), ginsenoside Rb3 content is 27.9%, and finger-print is as Fig. 5.
Embodiment 7---preparation promotes the composition of sleep
Rhizoma Gastrodiae powder 20g prepared by Example 1 and notoginseng haulm standard extract 2g prepared by embodiment 5, mixing, fully mixing obtains object.
Embodiment 8---preparation promotes the composition of sleep
Rhizoma Gastrodiae powder 50g prepared by Example 2 and notoginseng haulm standard extract 5g prepared by embodiment 4, mixing, fully mixing obtains object.
Embodiment 9---preparation promotes the composition of sleep
Rhizoma Gastrodiae powder 50g prepared by Example 3 and notoginseng haulm standard extract 5g prepared by embodiment 6, mixing, fully mixing obtains object.
Embodiment 10
The object that Example 7 prepares, is called in natural honey 200g, is fully mixed, be prepared as bee product, edible.
Embodiment 11
The object that Example 8 prepares, adds suitable vehicle, and well-established law is granulated, and compressing tablet, is prepared as irregular tablet, and for dyssomnia, crowd takes.
Embodiment 12
The object that Example 9 prepares, the part by weight with 1% joins in flour, makes various millet cake, as: bread, cake, noodles, the Flour product such as steamed bun, edible.
Embodiment 13
Pharmaceutical composition pack prepared by Example 7 becomes powder.
Embodiment 14
Pharmaceutical composition prepared by Example 9 adds pharmaceutically acceptable auxiliary material and makes tablet.
Embodiment 15
Pharmaceutical composition prepared by Example 8 adds pharmaceutically acceptable auxiliary material and makes capsule.
Embodiment 16
Pharmaceutical composition prepared by Example 8 adds pharmaceutically acceptable auxiliary material and makes granule.
Embodiment 17
Pharmaceutical composition prepared by Example 9 adds pharmaceutically acceptable auxiliary material and makes pill.
Embodiment 18
Pharmaceutical composition prepared by Example 7 adds pharmaceutically acceptable auxiliary material and makes honey pill agent.
Embodiment 19
Composition, on the impact of the yellow Jackets length of one's sleep, is tested with the composition of embodiment 8, experimental technique and result as follows:
Experiment material: male mouse of kunming, body weight 18-20g.Yellow Jackets (polygala root company is won in Beijing three)
Method: 40 mouse are divided at random the high, medium and low dosage group of composition, and blank group etc. 4 groups.The high, medium and low dosage component of composition does not give 166 mg/kg, 233 mg/kg, 466 mg/kg compositions, continuous gavage 7 days.Animal used as test is after last administration 30min, and lumbar injection 50mg/kg carbrital, records sleep latency, sleep time respectively.
Result: compare with blank group, the high, medium and low dosage of composition all can obvious Sleeping Time Prolong Ed, and p value is respectively 0.002,0.010 and 0165(table 1).
Conclusion: composition can work in coordination with the sleep of yellow Jackets inducing mouse, has good sedative-hypnotic effect.
The composition prolongation mouse amobarbital length of one's sleep prepared by table 1 embodiment of the present invention 8
Embodiment 20
Prepare composition test with embodiment 7, embodiment 9 respectively, test method is with embodiment 19, and result all shows that composition prepared by the present invention can work in coordination with the sleep of yellow Jackets inducing mouse, has good sedative-hypnotic effect.
Claims (10)
1. promote a composition of sleeping, it is characterized in that the composition of described promotion sleep comprises rhizoma Gastrodiae powder and Panax Notoginseng Folium Saponins, described rhizoma Gastrodiae powder and Panax Notoginseng Folium Saponins weight ratio are 8 ~ 12:1.
2. according to claim 1 promote sleep composition, it is characterized in that rhizoma Gastrodiae powder be with orchid rhizoma Gastrodiae (
gastrodia elatabl.) fresh tuber is raw material, and through steaming, dry and pulverising step prepares, and specifically comprises the following steps:
A, to steam: select fresh gastrodia elata, steam 5 ~ 10h under being placed in temperature 100 ~ 120 DEG C of steam for subsequent use;
B, drying: the rhizoma Gastrodiae after steaming is carried out being dried to water content 8 ~ 10% below temperature 60 C;
C, pulverizing: dried rhizoma Gastrodiae is carried out ultramicro grinding and cross 300 orders with top sieve, obtain object rhizoma Gastrodiae powder.
3. the composition promoting sleep according to claim 2, is characterized in that the drying described in step B is the one in low-temperature aeration drying, heated-air drying, heat drying or freeze drying.
4. the composition promoting sleep according to claim 1, it is characterized in that described Panax Notoginseng Folium Saponins is is that raw material prepares through raw material preparation, extraction, post-processing step with notoginseng haulm, specifically comprises the following steps:
A, raw material prepare: be equal to or higher than 7:3 by the weight ratio of sanchi leaf and pseudo-ginseng stem and carry out weighing preparation and obtain raw material notoginseng haulm;
B, extraction: get raw material notoginseng haulm, adding volume ratio is 4 ~ 5 times, concentration be 50 ~ 80% ethanol extract, filter, the medicinal extract a that filtrate is condensed into relative density 1.1 ~ 1.3 is for subsequent use;
C, post processing:
1) in medicinal extract a, adding water to relative density is 0.9 ~ 1.08, is heated to 70 ~ 80 DEG C, and adding with medicinal extract a weight ratio is the fining agent of 50:1-80:1, stir, place and naturally cool to room temperature, centrifugal, it is 0.9 ~ 1.08 obtain medicinal extract b that clarified solution after centrifugal is concentrated into relative density
2) in medicinal extract b, ethanolic solution is added, solution ethanol concentration is regulated to be 40 ~ 60%, stir, centrifugal, add the water of volume ratio 10:1-50:1 in the clarified solution after centrifugal, obtain solution a, by macroporous adsorption resin chromatography post, in succession carry out wash-out with solution a volume ratio 5-10 water, the ethanolic solution of solution a volume ratio 5:10 times of volumetric concentration 30% and the ethanolic solution of solution a volume ratio 5:10 times of volumetric concentration 80% doubly respectively, the eluent of collected volume concentration 80% ethanolic solution obtains eluent a
3) eluent a is again through ion exchange resin column column chromatography, carries out wash-out with eluent a volume ratio 5:10 7% ethanol doubly, collects eluent and obtains eluent b, by eluent b through concentrating, obtain object Panax Notoginseng Folium Saponins after drying.
5. the composition promoting sleep according to claim 4, is characterized in that the one be extracted as in refluxing extraction, ultrasonic wave extraction, Microwave Extraction, counter-flow extraction described in step A.
6. the composition promoting sleep according to claim 4, is characterized in that the fining agent described in step B is ZTC 1+1, gelatin or shitosan.
7. the composition promoting sleep according to claim 4, is characterized in that the filler of the macroporous adsorption resin chromatography post described in step C is the one in D-101, DA-201, D1400, CAD-40, ADS-5, HPD-500, DM130, XAD-2, XAD-4, Diaion or AB-8 model macroporous absorbent resin; The filler that described resinbed analyses post is the one in D941, D280, D296, D900, D396, D390, D311 or D314 model anion exchange resin.
8., according to the arbitrary described preparation method promoting the composition of sleep of claim 1 ~ 7, it is characterized in that the rhizoma Gastrodiae powder prepared and Panax Notoginseng Folium Saponins to be mixed to get by formula rate.
9., according to the arbitrary described formulation method promoting the composition of sleep of claim 1 ~ 7, it is characterized in that adding the auxiliary material pharmaceutically accepted in the composition of described promotion sleep is prepared into powder, tablet, capsule, granule, pill or honey pill agent.
10., according to the arbitrary described application promoting the composition of sleep of claim 1 ~ 7, it is characterized in that the application in the health-oriented products of composition in preparation promotion sleep of described promotion sleep, food additives, health food.
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| CN111184731A (en) * | 2020-01-21 | 2020-05-22 | 西安巨子生物基因技术股份有限公司 | Application of ginsenoside Rg5 in preparation of pharmaceutical composition for improving sleep |
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| Title |
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| 耿家玲等: "水提三七叶苷胶囊改善睡眠实验研究", 《云南中医中药杂志》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111184731A (en) * | 2020-01-21 | 2020-05-22 | 西安巨子生物基因技术股份有限公司 | Application of ginsenoside Rg5 in preparation of pharmaceutical composition for improving sleep |
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Application publication date: 20150826 |