CN104855733A - Application of yeast containing active polysaccharides and nucleotides in dairy cow feed - Google Patents
Application of yeast containing active polysaccharides and nucleotides in dairy cow feed Download PDFInfo
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- CN104855733A CN104855733A CN201510315328.7A CN201510315328A CN104855733A CN 104855733 A CN104855733 A CN 104855733A CN 201510315328 A CN201510315328 A CN 201510315328A CN 104855733 A CN104855733 A CN 104855733A
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Abstract
The invention discloses the application of a yeast containing active polysaccharides and nucleotides in a dairy cow feed. The yeast containing active polysaccharides and nucleotides contains such active polysaccharide components as glucan, mannan and mannan oligosaccharide, and the total content of the active polysaccharides is 1-20wt% of that of the yeast; the yeast also contains such nucleotides as adenylate, cytidine monophosphate, guanylic acid and uridylic acid, and the total content of the nucleotides is 1-20wt% of that of the yeast. According to the method, the active feed is added to the dairy cow feed, the serum albumin of a calf can be increased by 140mg/dL by use of the methods of optimizing charging and feeding conditions and the like, and the maximum mutation rate of the rotavirus in the colostrum can be 75%. The application of the yeast containing active polysaccharides and nucleotides in the dairy cow feed has the advantages that 1, the yeast feed is capable of increasing the content of the serum albumin of the calf and boosting the immunity of the calf, and 2, the yeast feed is capable of mutating a large amount of rotavirus in the colostrum and preventing the rotavirus from pathopoiesis.
Description
Technical field
The invention belongs to technological field of biochemistry, be specifically related to the novel yeast feed containing active polysaccharide (glucan, mannosan, manna oligosacchride) and nucleotides (adenylate, cytidine monophosphate, guanylic acid, uridylic acid) prepared by the industrial waste yeast of a kind of use.
Background technology
Containing abundant nutritional labeling in yeast cells, except containing multiple essential amino acid and essential fatty acid, abundant vitamin, mineral matter, multiple digestive ferment and growth stimulant etc.Also containing a certain amount of RNA and a large amount of active polysaccharide-beta glucans and manna oligosacchride.
Yeast cells is nutritious, but yeast cell wall component is complicated, and intracellular nutritional material and physiological activator are difficult to effectively be absorbed by animal, and major part is discharged outside animal body as discarded object.Broken wall yeast adopts physics or biochemical method broken yeast cell wall, makes a kind of product that yeast cell contents is fully discharged into outside born of the same parents and macromolecular substances is suitably degraded.Yeast cells is after fragmentation, the physiological activators such as the nutriments such as intracellular protein, amino acid, nucleic acid, vitamin and glutathione, trehalose, superoxide dismutase (SOD) are released to outside born of the same parents, in cell membrane, glucan and mannosan are oligosaccharides by decomposed, protein portion is decomposed into polypeptide, trophism and the functional (Wang Pengyin of raising all greatly, Wang Haiyan etc., the research and apply [J] of saccharomyces cerevisiae source feed additive, feed addictive, 2011,32 (2): 30-35).
Yeast and product thereof can strengthen aquatic livestock immunologic function to have research to point out, improve aquatic livestock resistance against diseases, improve survival rate of seedling.Containing many active components in yeast, as beta glucan and manna oligosacchride.They exercise respective function in animal body respectively, the disease that can cause bacterium, fungi and virus and transport, inoculation, weather etc. change the stress reaction caused and produce non-specific immunity (Meng Siyu, Meng Changming etc., the application prospect of yeast product in aquatic feeds [J], fishery is got rich guide, 2010,18:56-57).
Beta glucan energy stimulating animal body produces the macrophage played a crucial role to body's immunity, the pathogenic microorganism in the nucleus intrusive body of can damage in purged body, becoming feeble and die.Manna oligosacchride can promote the hyperplasia of beneficial bacterium, and beneficial bacterium is good immunoactivator, effectively can improve the activity of anti-interferon and macrophage, the immune stimulatories such as action activity are bitten with raising is huge by producing antibody, thus excitating organism humoral and cellular immune response, Promote immunity allelotaxis and T lymphocyte and bone-marrow-derived lymphocyte increase, thus strengthen the immunologic function (Zhang Hongmei of body, Jiang Huimin, yeast manna oligosacchride is on the impact [J] of carp nospecific immunity, feed is studied, 2006,10:9-11).
Nucleotides is the base unit of the inhereditary material in organism, is the monomer forming RNA, is also the required intermediate of albumen synthesis, and has important function in eucaryotic cell structure, metabolism, energy and function point analysis.There are some researches prove that nucleotides has promote the growth of poultry and improve its immunologic function (to owl, Zhou Xinghua etc., yeast nucleotides on carp growth performance, body composition and the impact [J] of sero-immunity index, Animal nutrition journal 2011,23 (1): 171-178).
Immunologic function is the basis maintaining milk cow body health; the generation of the various disease of milk cow is all relevant with immunologic function; if immunosupress appears in body, will cause the generation of various disease, the generation of these diseases can reduce production performance and reproductive performance; affect milk quality and cause mortality to raise; thus cause huge economic loss (Xue Junxin, Zhang Kechun, the influence factor [J] of immunity of cow to aquaculture; dairy industry science and technology, 2011.3:142-144).
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of yeast feed containing active polysaccharide and nucleotides.By giving this feed of milk cow feeding, milk cow and nascent calf immunity can be increased, the pollution of Industry Waste yeast to environment can be solved again.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
Contain the application of yeast in milk cow forage for active polysaccharide and nucleotides,
Wherein, the described yeast containing active polysaccharide and nucleotides, be glucan, mannosan and manna oligosacchride containing active polysaccharide composition, active polysaccharide total content is the 1 ~ 20wt% (preferably 3 ~ 15wt%) of yeast, composition containing nucleotides is adenylate, cytidine monophosphate, guanylic acid and uridylic acid, and the total content of nucleotides is the 1 ~ 20wt% (preferably 5 ~ 15wt%) of yeast.
Wherein, the described addition of yeast in milk cow forage containing active polysaccharide and nucleotides is 2 ~ 50wt% of bundle feed dry weight, preferably 5 ~ 15wt%.
Wherein, the described yeast containing active polysaccharide and nucleotides prepares as follows: by fermentation by saccharomyces cerevisiae, fermentation liquor Separation of Solid and Liquid, solid portion adds water and is made into yeast juice, the Mannoproteins that interpolation neutral proteinase and nuclease P 1 enzymatic yeast contain self and ribonucleic acid, thus obtain the yeast containing active polysaccharide and nucleotides.
Wherein, fermentation by saccharomyces cerevisiae condition is: fermentation medium culture medium prescription: fermentation medium culture medium prescription: glucose 5 ~ 50g/L, (NH
4)
2sO
41 ~ 10g/L, H
3pO
41 ~ 10ml/L, MgSO
41 ~ 10g/L, FeSO
40.5 ~ 5g/L, is adjusted to 3 ~ 8 with ammoniacal liquor by medium pH; 50 ~ 300rpm fermented and cultured 10 ~ 30 hours at 25 ~ 40 DEG C.Preferred fermentative medium formula is: glucose 30g/L, (NH
4)
2sO
44g/L, H
3pO
41.5ml/L, MgSO
41.5g/L, FeSO
40.8g/L, is adjusted to 4.0 with ammoniacal liquor by medium pH.Preferred fermentation by saccharomyces cerevisiae condition is: 100rpm fermented and cultured 18 hours at 32 DEG C.
Wherein, fermentation liquor Separation of Solid and Liquid, solid portion adds water and is made into the yeast juice that dry solid content is 10 ~ 18wt%, preferred 15wt%.
Wherein, after solid portion adds water and is made into yeast juice, be warming up to 60 ~ 100 DEG C, 30 ~ 70 DEG C are cooled to after insulation 0.5 ~ 5h, respectively add the neutral proteinase of 0.1%g/mL content and the nuclease P 1 reaction 2 ~ 8h of 0.1%v/v content again, the Mannoproteins that enzymatic yeast self contains and ribonucleic acid, reaction terminates the dry powdered of rear spray, obtains the yeast containing active polysaccharide and nucleotides.Preferably, after solid portion adds water and is made into yeast juice, be warming up to 90 DEG C, be incubated and be cooled to 60 DEG C after 30 minutes, add the neutral proteinase of 0.1%g/mL content and the nuclease P 1 reaction 5h of 0.1%v/v content again, the Mannoproteins that enzymatic yeast self contains and ribonucleic acid, reaction terminates the dry powdered of rear spray, obtains the yeast containing active polysaccharide and nucleotides.
Wherein, described neutral proteinase, the work of its enzyme is 10000 ~ 100000U/g; Described nuclease P 1, the work of its enzyme is 500 ~ 5000U/ml.
Neutral proteinase enzyme is lived and is defined as: 1g solid enzyme powder, at 30 DEG C, under pH7.5 condition, it is an enzyme activity unit that 1 minute caseinhydrolysate produces 1 μ g tyrosine, represents with U/g.
Nuclease P 1 enzyme is lived and is defined as: at 37 DEG C, enzymolysis ribonucleic acid under pH5.3, and the enzyme amount of the acid-soluble nucleotides of release in 1 minute 1.0 μm of ol is an enzyme activity unit, represents with U/ml.
Wherein, this feed applications before cow birth 1 ~ 4 week until childbirth, before preferred cow birth 4 weeks until give a birth.
Wherein, with the calf obtained after the cow birth of this diet, its seralbumin amount is 180 ~ 410mg/dL.
Wherein, with the cow birth of this diet, its first Ruzhong rotavirus mutation rate is 37 ~ 75%.
Based on the immunologic function of active polysaccharide in waste yeast and nucleotides, it can be used as the feed of milk cow, the nospecific immunity of milk cow can not only be improved, its case fatality rate is declined, the problem of Industry Waste yeast contaminated environment can be solved again.
Beneficial effect: superiority of the present invention is:
1, along with this yeast feed is as the prolongation of feed service time of milk cow, calf serum albumin content is added.
2, this yeast feed makes rotavirus in colostrum make a variation to a certain extent as the feed of milk cow, makes it not pathogenic.
Detailed description of the invention
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
In following examples, seralbumin ELISA kit detects, and rotavirus mutation rate GICA method detects.
Embodiment 1:
Saccharomyces cerevisiae (CGMCC No.7522) bacterial strain is linked in 10T fermentation tank and is cultivated, and culture medium is: glucose 30g/L, (NH
4)
2sO
44g/L, H
3pO
41.5ml/L, MgSO
41.5g/L, FeSO
40.8g/L, is adjusted to 4.0 with ammoniacal liquor by medium pH, 50 ~ 300rpm fermented and cultured 10 ~ 30 hours at 25 ~ 40 DEG C.Carry out Separation of Solid and Liquid with horizontal spiral separator, the yeast paste obtained adds water and is made into the yeast liquid that dry solid content is 15%.Be warming up to 90 DEG C, be incubated and be cooled to 60 DEG C after 30 minutes.Add neutral proteinase (enzyme 30000U/ml alive) and the 0.1%v/v nuclease P 1 (enzyme 2200U/ml alive) of 0.1%g/mL content respectively, after 5h, reaction terminates, just be sprayed into powder with spray dryer, obtained the yeast containing active polysaccharide and nucleotides.
Embodiment 2:
Choose 20 body conditions close, before childbirth milk cow pairing be around divided into two groups, control group feeds basal feed, and (basal feed formula is: corn 60wt%, dregs of beans 26.0wt%, expanded soybean 6.0wt%, wheat bran 5.0wt%, imported fish meal 2.0wt%, soybean oil 1.0wt%, following examples are identical), the yeast (preparing according to the method for embodiment 1) containing active polysaccharide and nucleotides that test group adds 10wt% carrys out alternative equivalent basal feed.Wherein in yeast, active polysaccharide content is 3wt%, and nucleotide content is 5wt%.Test until point puerperium, gets calf colostrum sample and calf blood sample from giving a birth first 1 week.Experimental group calf serum albumin content is 280mg/dL, and control group is 270mg/dL.First Ruzhong rotavirus is 37% relative to the mutation rate of control group.
Embodiment 3:
Choose 20 body conditions close, before childbirth milk cow pairing be around divided into two groups, control group feeds basal feed, and the yeast (preparing according to the method for embodiment 1) containing active polysaccharide and nucleotides that test group adds 15wt% carrys out alternative equivalent basal feed.Wherein in yeast, active polysaccharide content is 5wt%, and nucleotide content is 10wt%.Test until point puerperium, gets calf colostrum sample and calf blood sample from giving a birth first 2 weeks.Calf serum albumin content is 370mg/dL, and control group is 270mg/dL.First Ruzhong rotavirus is 52% relative to the mutation rate of control group.
Embodiment 4:
Choose 20 body conditions close, before childbirth milk cow pairing be around divided into two groups, control group feeds basal feed, and the yeast (preparing according to the method for embodiment 1) containing active polysaccharide and nucleotides that test group adds 5wt% carrys out alternative equivalent basal feed.Wherein in yeast, active polysaccharide content is 15wt%, and nucleotide content is 15wt%.Test until point puerperium, gets calf colostrum sample and calf blood sample from giving a birth first 3 weeks.Calf serum albumin content is 390mg/dL, and control group is 270mg/dL.First Ruzhong rotavirus is 61% relative to the mutation rate of control group.
Embodiment 5:
Choose 20 body conditions close, before childbirth milk cow pairing be around divided into two groups, control group feeds basal feed, and the yeast (preparing according to the method for embodiment 1) containing active polysaccharide and nucleotides that test group adds 8wt% carrys out alternative equivalent basal feed.Wherein in yeast, active polysaccharide content is 10wt%, and nucleotide content is 5wt%.Test until point puerperium, gets calf colostrum sample and calf blood sample from giving a birth first 4 weeks.Calf serum albumin content is 410mg/dL, and control group is 270mg/dL.First Ruzhong rotavirus is 75% relative to the mutation rate of control group.
Embodiment 6:
Choose 20 body conditions close, before childbirth milk cow pairing be around divided into two groups, control group feeds basal feed, and the active polysaccharide (glucan, mannosan, manna oligosacchride account for 1/3) that test group adds 0.8wt% carrys out alternative equivalent basal feed.Test until point puerperium, gets calf colostrum sample and calf blood sample from giving a birth first 4 weeks.Calf serum albumin content is 340mg/dL, and control group is 270mg/dL.First Ruzhong rotavirus is 56% relative to the mutation rate of control group.
Embodiment 7:
Choose 20 body conditions close, before childbirth milk cow pairing be around divided into two groups, control group feeds basal feed, and the nucleotides (adenylate, cytidine monophosphate, guanylic acid, uridylic acid respectively account for 1/4) that test group adds 0.4wt% carrys out alternative equivalent basal feed.Test until point puerperium, gets calf colostrum sample and calf blood sample from giving a birth first 4 weeks.Calf serum albumin content is 380mg/dL, and control group is 270mg/dL.First Ruzhong rotavirus is 60% relative to the mutation rate of control group.
Claims (10)
1. contain the application of yeast in milk cow forage for active polysaccharide and nucleotides,
Wherein, the described yeast containing active polysaccharide and nucleotides, be glucan, mannosan and manna oligosacchride containing active polysaccharide composition, active polysaccharide total content is 1 ~ 20wt% of yeast, composition containing nucleotides is adenylate, cytidine monophosphate, guanylic acid and uridylic acid, and the total content of nucleotides is 1 ~ 20wt% of yeast.
2. application according to claim 1, is characterized in that, the described addition of yeast in milk cow forage containing active polysaccharide and nucleotides is 2 ~ 50wt% of bundle feed dry weight.
3. application according to claim 1, it is characterized in that, the described yeast containing active polysaccharide and nucleotides prepares as follows: by fermentation by saccharomyces cerevisiae, fermentation liquor Separation of Solid and Liquid, solid portion adds water and is made into yeast juice, the Mannoproteins that interpolation neutral proteinase and nuclease P 1 enzymatic yeast contain self and ribonucleic acid, thus obtain the yeast containing active polysaccharide and nucleotides.
4. application according to claim 3, is characterized in that, fermentation by saccharomyces cerevisiae condition is: fermentation medium culture medium prescription: glucose 5 ~ 50g/L, (NH
4)
2sO
41 ~ 10g/L, H
3pO
41 ~ 10ml/L, MgSO
41 ~ 10g/L, FeSO
40.5 ~ 5g/L, is adjusted to 3 ~ 8 with ammoniacal liquor by medium pH; 50 ~ 300rpm fermented and cultured 10 ~ 30 hours at 25 ~ 40 DEG C.
5. application according to claim 3, is characterized in that, fermentation liquor Separation of Solid and Liquid, and solid portion adds water and is made into the yeast juice that dry solid content is 10 ~ 18wt%.
6. application according to claim 3, it is characterized in that, after solid portion adds water and is made into yeast juice, be warming up to 60 ~ 100 DEG C, be cooled to 30 ~ 70 DEG C after insulation 0.5 ~ 5h, then add the neutral proteinase of 0.1%g/mL content and the nuclease P 1 reaction 2 ~ 8h of 0.1%v/v content, the Mannoproteins that enzymatic yeast self contains and ribonucleic acid, reaction terminates the dry powdered of rear spray, obtains the yeast containing active polysaccharide and nucleotides.
7. application according to claim 6, is characterized in that, described neutral proteinase, and the work of its enzyme is 10000 ~ 100000U/g; Described nuclease P 1, the work of its enzyme is 500 ~ 5000U/ml.
8. application according to claim 1, is characterized in that, this feed applications before cow birth 1 ~ 4 week until childbirth.
9. application according to claim 4, is characterized in that, with the calf obtained after the cow birth of this diet, its seralbumin amount is 180 ~ 410mg/dL.
10. application according to claim 4, is characterized in that, after the cow birth of this diet, the rotavirus mutation rate in its first Ruzhong is 37 ~ 75%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108378243A (en) * | 2018-05-17 | 2018-08-10 | 南京同凯兆业生物技术有限责任公司 | A kind of feed for sow additive and its application |
| CN116746637A (en) * | 2023-07-20 | 2023-09-15 | 南京工业大学 | Feed additive for calves and preparation method and application thereof |
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2015
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Cited By (3)
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|---|---|---|---|---|
| CN108378243A (en) * | 2018-05-17 | 2018-08-10 | 南京同凯兆业生物技术有限责任公司 | A kind of feed for sow additive and its application |
| CN108378243B (en) * | 2018-05-17 | 2021-05-25 | 南京同凯兆业生物技术有限责任公司 | Feed additive for sows and application thereof |
| CN116746637A (en) * | 2023-07-20 | 2023-09-15 | 南京工业大学 | Feed additive for calves and preparation method and application thereof |
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