CN104839429B - The method that the combined solid fermented preparation of steam blasting is rich in fatty acid feedstuff - Google Patents
The method that the combined solid fermented preparation of steam blasting is rich in fatty acid feedstuff Download PDFInfo
- Publication number
- CN104839429B CN104839429B CN201510290665.5A CN201510290665A CN104839429B CN 104839429 B CN104839429 B CN 104839429B CN 201510290665 A CN201510290665 A CN 201510290665A CN 104839429 B CN104839429 B CN 104839429B
- Authority
- CN
- China
- Prior art keywords
- trichosporon cutaneum
- fatty acid
- solid
- rich
- carries out
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses the methods that a kind of combined solid fermented preparation of steam blasting is rich in fatty acid feedstuff, and starch biomass material is packed into Steam explosive machine and carries out explosion；Material after collection explosion, controls temperature, and wheat bran is added, and adjusts water content and pH value, and medium temperature amylase is added and carbohydrase carries out solid-state saccharification to it；By the sticky trichosporon cutaneum strain inoculated and cultured of activation at sticky trichosporon cutaneum seed liquor, then the viscosity trichosporon cutaneum seed liquor is inoculated in the solid material after saccharification by certain inoculum concentration and carries out solid state fermentation；Tunning is dry, crushing, can be obtained feed product.The present invention comprehensively considers biology and physics processing method, to starch materials elder generation explosion treatment and saccharification, it adds sticky trichosporon cutaneum and carries out solid fermentation, the feedstuff rich in fatty acid of high quality has been produced using lower cost, provides a kind of more cost-effective energy feed feedstock production process for the development of animal husbandry.
Description
Technical field
The present invention relates to biological feedstuff production technical fields, more particularly, to a kind of combined solid fermented preparation of steam blasting
Method rich in fatty acid feedstuff.
Background technique
The purpose that people initially add grease in animal diets is to provide high concentration energy, meets fast growth of animal mistake
To the demand of energy in journey.With Animal nutrition research deeply and grease being widely used in feed, it has been found that oil
The more use value of rouge, for example essential fatty acid (linoleic acid and a- linolenic acid) must be added in animal rapid growth feed
, it can promote the absorption of the nutritional ingredients such as fat-soluble pigment and microelement；Pleasant incense oil taste can be brought to increase suitable
Mouth property；Increase the stability of aquatic feeds in water；The generation of dust and the energy consumption of feed granulating during reduction feed processing.
Grease used in animal and fowl fodder is mostly animality and vegetative grease at present, due to China be well-known Feed Manufacturing and
Using big country, the vegetable oil rise in price that vegetable fat and animal fat application in feed formula cause the mankind to eat, valence
The dynamic variation for driving feed, agricultural product and its byproduct price of lattice wave, increases the risk of animal husbandry.Therefore new in exploitation
Oil resource have become problem anxious to be resolved at present.
Microbial oil (Microbial oils) is also known as Unicell Oils and Fats, is utilized by microorganisms such as algae, Mycophytas
Carbohydrate, hydrocarbon and common grease are carbon source, and grease storage is converted into thallus.Microbial oil is being just at present
Widely studied and applied, it has vegetable fat and the incomparable advantage of animal fat: 1. microbial oil be can
Regenerated resources；2. microbial oil is higher than nutritive values such as soybean oil, palm oil and sunflower oils；3. using starchiness class
Low cost feedstock is produced；4. containing plant and animal cannot or the fatty acid that synthesizes, such as linoleic acid, linolenic acid on a small quantity.
Steam blasting pretreatment is a kind of most promising pretreatment of raw material technology developed in recent years, and principle is logical
Demolition set is crossed, certain pressure is heated the raw material to using saturated steam, steam is made to penetrate into material group under pressure
Knit inside configuration, formed powerful compressed gas can and in a short time explosion discharge, so that it is big to break material from molecular level
Molecular lattice structure, physical structure and chemical composition to material have very strong reconstruct effect, are convenient for subsequent processing and application.
Summary of the invention
The present invention provides a kind of method that the combined solid fermented preparation of steam blasting is rich in fatty acid feedstuff, and purpose exists
It is cheap and rich in fat, fatty acid to obtain in by two methods joining together to handle starch biolobic material
Animal feed raw material.
To achieve the above object, the present invention can take following technical proposals:
Combined solid fermented method of the preparation rich in fatty acid feedstuff of steam blasting of the present invention includes following
Step:
The starch biomass material that water content is 10~15% is packed into Steam explosive machine by the first step, it is passed through 160 after sealing~
215 DEG C of saturated vapor maintains 0.5~5min after pressure reaches 0.4~1.0MPa, the steam explosion is opened in 0.00875s
The operated pneumatic valve of machine carries out explosion；
Second step collects material after explosion, controls temperature of charge at 50~60 DEG C, by weight the bran for being added 40~50%
Skin, adjustment water content are 40~50%, while with Ca (OH)2PH value 6.0~7.5 is adjusted, medium temperature amylase and carbohydrase is added
It is carried out spare after solid-state saccharification；
Third step glues the sticky trichosporon cutaneum strain inoculated and cultured of activation at sticky trichosporon cutaneum seed liquor, then by this
Sex pilus spore yeast starter liquid is inoculated in the solid material after second step saccharification by 10% inoculum concentration and carries out solid state fermentation；
4th step, tunning is dry, crushing, can be obtained rich in fatty acid feedstuff product.
The starch biomass material is one or more of corn, wheat and sorghum.
The dosage for the medium temperature amylase being added in the second step is 50~80U/g, the dosage of the carbohydrase is 210~
250U/g；The solid-state saccharificatinn period is 8~12h.
Solid state fermentation conditions in the third step are as follows: 28~30 DEG C of fermentation temperature, the initial pH that ferments is 5.0~6.5, hair
The ferment period is 4~8d.
The incubation of sticky trichosporon cutaneum seed liquor in the third step are as follows: inclined-plane picking viscosity trichosporon cutaneum strain connects
Kind enters culture bottle, and culture obtains primary seed solution；Then it is expanded in seed liquid culture medium by second level culture again, obtains viscosity
Trichosporon cutaneum secondary seed solution；The seed liquid culture medium composition are as follows: 15 g/L of glucose, (NH4)2SO42.5 g/L, yeast
Cream l g/L, peptone 7 g/L, KH2PO4 7 g/L、Na2HPO4 2 g/L、MgSO4·7H2O 2 g/L, pH 6.0.
The present invention comprehensively considers biology and physics processing method, to the starch materials such as corn, wheat and sorghum elder generation
After carrying out explosion treatment and saccharification, adds sticky trichosporon cutaneum and carry out solid fermentation, height has been produced using lower cost
The feedstuff rich in fatty acid of quality, while reducing the generation of discharge of wastewater and harmful substance, it is animal husbandry
Development provides a kind of more cost-effective energy feed feedstock production process.
The present invention uses steam blasting equipment first, using compressed gas energy powerful in detonation, by starch biolobic material
Raw material explosion pulp, and maturing process is completed, subsequent saccharificatinn period is substantially reduced, improves starch to glucide
Conversion ratio.After completing steam explosion technique, the present invention again consolidates the steam blasting material after saccharification using sticky trichosporon cutaneum
State fermentation, yeast are calculated, grease contains in obtained finished product using sugar and other starch ingredients generation grease after saccharification
Amount reaches 8.6%~12.8%, and content of fatty acid reaches 6.5%~9.5%, provides the approach of a new oil resource of exploitation.
Note: sticky trichosporon cutaneum (Trichosporon mucoides) strain be purchased from Chinese industrial Microbiological Culture Collection
Administrative center, number CICC32858.
Specific embodiment
Carry out detailed elaboration preparation method of the invention below by specific embodiment.
Viscosity trichosporon cutaneum seed liquor used in the present invention, is prepared by the following method: by the sticky silk spore ferment of activation
Female inclined-plane picking proper amount of strains, access fill in 250 mL triangular flasks of 25 mL seed liquid culture mediums, revolving speed 150 r/min, and 28
DEG C 22 h of shaken cultivation obtains primary seed solution.Then using 2 grades of amplification cultivations, sticky trichosporon cutaneum second level can be obtained
Seed liquor；The condition of culture of secondary seed is as follows: culture medium is still seed liquid culture medium, and 28~30 DEG C of cultivation temperature, culture is all
Phase be 22~for 24 hours, inoculum concentration 10%.The composition of seed liquid culture medium used in it are as follows: 15 g/L of glucose, (NH4)2SO4
2.5 g/L, yeast extract l g/L, peptone 7 g/L, KH2PO4 7 g/L、Na2HPO4 2 g/L、MgSO4·7H22 g/L of O,
pH 6.0。
Embodiment 1
The method that the combined solid fermented preparation of steam blasting of the present invention is rich in fatty acid feedstuff is specific to walk
It is rapid as follows:
It a. will be spare after the wheat dedusting of moisture content 12wt%；
B. wheat materials are sent into Steam explosive machine, 185 DEG C of saturated vapors is passed through after closing, when the quick-fried in-cylinder pressure of Steam explosive machine reaches
To after 0.5MPa, 1min is maintained, the operated pneumatic valve that Steam explosive machine is opened in 0.00875s carries out explosion；
C. the material after explosion is collected, when temperature of charge is down to 50 DEG C, by weight the wheat bran for being added 50%, adjustment contains
Water is 45%, while with Ca (OH)2Adjusting pH is 6.0, then respectively by the addition of the amount of 50 U/g raw materials and 210U/g raw material
Warm amylase and carbohydrase, after mixing evenly solid-state be saccharified 12h；
D. by the material after saccharification with Ca (OH)2PH value is adjusted to 5.8, accesses cultured sticky trichosporon cutaneum seed
Liquid, inoculum concentration 10%, 30 DEG C of fermentation 6d；
E. by the material after fermentation by drying and the obtained feedstuff rich in fatty acid that pulverizes and sieves.
After tested, fat content reaches 8.6% in gained feedstuff product, and content of fatty acid reaches 6.5%.
Embodiment 2
The method that the combined solid fermented preparation of steam blasting of the present invention is rich in fatty acid feedstuff, specific steps
It is:
A. it by corn dedusting after natural drying, measures spare after 11.2 wt% of moisture content；
B. maize raw material is sent into Steam explosive machine, 185 DEG C of saturated vapors is passed through after closing, when the quick-fried in-cylinder pressure of Steam explosive machine reaches
To after 0.5 MPa, 4min is maintained, the operated pneumatic valve that Steam explosive machine is opened in 0.00875s carries out explosion；
C. material, by weight the wheat bran for being added 50%, adjusts aqueous after temperature of charge is down to 60 DEG C after collecting explosion
Amount is 42%, while with Ca (OH)2Adjusting pH is 6. 5, and medium temperature starch is added by the amount of 80U/g raw material and 250U/g raw material respectively
Enzyme and carbohydrase, after mixing evenly, 8 h of solid-state saccharification；
D. by the material after saccharification with Ca (OH)2PH value is adjusted to 5.8, accesses sticky trichosporon cutaneum seed liquor, inoculum concentration
10%, 28 DEG C of fermentation 6d；
E. by the material after fermentation by drying and the obtained feedstuff rich in fatty acid that pulverizes and sieves.
After tested, fat content reaches 10.2% in gained feedstuff product, and content of fatty acid reaches 8.16%.
Embodiment 3
The method that the combined solid fermented preparation of steam blasting of the present invention is rich in fatty acid feedstuff, specific steps
It is:
A. it by sorghum dedusting after natural drying, measures spare when moisture content is 11.0 wt%；
B. sorghum material is packed into Steam explosive machine, the saturated vapor that temperature is 215 DEG C is passed through after closing, in Steam explosive machine detonation
Pressure maintains 4min after reaching 1.0MPa, and the operated pneumatic valve that Steam explosive machine is opened in 0.00875s carries out steam blasting；
C. 40% wheat bran is added after temperature is down to 50 DEG C in material by weight after collecting explosion, and adjustment water content is
41%, while with Ca (OH)2Adjusting pH is 6. 5, and medium temperature then is added by the amount of 75 U/g raw materials and 220 U/g raw materials respectively and forms sediment
Powder enzyme and carbohydrase, after mixing evenly, 10 h of solid-state saccharification；
D. by the material after saccharification with Ca (OH)2PH value is adjusted to 5.5, accesses sticky trichosporon cutaneum seed liquor, inoculum concentration
10%, 30 DEG C of fermentation 6d.
E. by the material after fermentation by drying and the obtained feedstuff rich in fatty acid that pulverizes and sieves.
After tested, fat content reaches 9.6% in gained feedstuff product, and content of fatty acid reaches 7.7%.
Steam explosive machine in above embodiments can be using the steam explosion testing equipment of Hebi right way bioenergy company production, institute
It is commercial product with medium temperature amylase and carbohydrase.
Claims (2)
1. a kind of method that the combined solid fermented preparation of steam blasting is rich in fatty acid feedstuff, it is characterised in that comprising following
Step:
The starch biomass material that water content is 10~15% is packed into Steam explosive machine, 160~215 DEG C is passed through after sealing by the first step
Saturated vapor, after quick-fried in-cylinder pressure reaches 0.5~1.0MPa maintain 0.5~5min, the vapour is opened in 0.00875s
The operated pneumatic valve of quick-fried machine carries out explosion；
Second step collects material after explosion, controls temperature of charge at 50~60 DEG C, by weight the wheat bran for being added 40~50%, adjusts
Whole water content be 40~50%, while with Ca (OH) 2 adjust pH value 6.0~7.5, addition medium temperature amylase and carbohydrase to its into
Spare after the saccharification of row solid-state, wherein the dosage of medium temperature amylase is 50~80U/g, and the dosage of the carbohydrase is 210~250U/
g；The solid-state saccharificatinn period is 8~12h；
Third step, by the sticky trichosporon cutaneum strain inoculated and cultured of activation at sticky trichosporon cutaneum seed liquor, then by the viscosity silk
Spore yeast starter liquid is inoculated in the solid material after second step saccharification by 10% inoculum concentration and carries out solid state fermentation, solid state fermentation
Condition are as follows: 28~30 DEG C of fermentation temperature, the initial pH that ferments is 5.0~6.5, and fermentation period is 4~8d；
The incubation of its sticky trichosporon cutaneum seed liquor are as follows: inclined-plane picking viscosity trichosporon cutaneum strain is inoculated with into culture bottle, training
It supports and obtains primary seed solution；Then it is expanded in seed liquid culture medium by second level culture again, obtains sticky trichosporon cutaneum second level
Seed liquor；The seed liquid culture medium composition are as follows: 15 g/L of glucose, 2.5 g/L of (NH4) 2SO4, yeast extract l g/L, albumen
772 g/L of g/L, Na2HPO4 of g/L, KH2PO4 of peptone, MgSO47H2 O 2 g/L, pH 6.0；
4th step, tunning is dry, crushing, can be obtained feed product.
2. the method that the combined solid fermented preparation of steam blasting according to claim 1 is rich in fatty acid feedstuff,
Be characterized in that: the starch biomass material is one or more of corn, wheat and sorghum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510290665.5A CN104839429B (en) | 2015-05-28 | 2015-05-28 | The method that the combined solid fermented preparation of steam blasting is rich in fatty acid feedstuff |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510290665.5A CN104839429B (en) | 2015-05-28 | 2015-05-28 | The method that the combined solid fermented preparation of steam blasting is rich in fatty acid feedstuff |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104839429A CN104839429A (en) | 2015-08-19 |
| CN104839429B true CN104839429B (en) | 2019-03-15 |
Family
ID=53839728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510290665.5A Expired - Fee Related CN104839429B (en) | 2015-05-28 | 2015-05-28 | The method that the combined solid fermented preparation of steam blasting is rich in fatty acid feedstuff |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104839429B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105767912A (en) * | 2016-01-14 | 2016-07-20 | 河南农业大学 | Method for removing anti-nutritional factors from bean meal |
| CN115226810B (en) * | 2022-07-21 | 2023-10-31 | 中国农业大学 | Preparation method of solid state fermentation feed |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101768606A (en) * | 2010-02-24 | 2010-07-07 | 中国科学院过程工程研究所 | Method for solid state fermentation of ethanol through steam explosion of cassava and comprehensive utilization of cassava |
| CN102002516A (en) * | 2010-11-09 | 2011-04-06 | 河南农业大学 | Novel method for producing ethanol through steam explosion of cereal starchy material |
| CN102077903B (en) * | 2010-11-25 | 2013-01-23 | 河南农业大学 | Method for jointly treating stalks by steam explosion and microorganism fermentation |
| CN102210383A (en) * | 2011-04-08 | 2011-10-12 | 甘肃圣大方舟马铃薯变性淀粉有限公司 | Method for preparing mycoprotein feed from waste water and waste residues in processing process of potatoes |
| CN103005147B (en) * | 2012-10-29 | 2014-10-08 | 重庆百奥帝克微生态科技有限公司 | Biological feed based on vinasse |
-
2015
- 2015-05-28 CN CN201510290665.5A patent/CN104839429B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN104839429A (en) | 2015-08-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103396245B (en) | Pleurotus cornucopiae cultivation material compatibility and production method of pleurotus cornucopiae cultivation material | |
| CN103145462B (en) | Enoki mushroom stock culture material and making method thereof | |
| CN103333022B (en) | Cultivation material compatibility of agrocybe cylindracea and manufacture method of cultivation material | |
| CN1931004B (en) | Process for producing high protein feed with paper-mulberry leaf and distiller's grains | |
| CN107417475A (en) | A kind of agro-ecology fertilizer and preparation method thereof | |
| CN104041717A (en) | Special feed, prepared from distiller's grains, for laying hen during egg producing period and preparation method of feed | |
| CN102823726A (en) | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity | |
| CN104782892B (en) | A kind of sugarcane residue fermented feed and preparation method thereof | |
| CN103343104B (en) | Process for preparing novel plant-source probiotics solution | |
| CN104472855A (en) | Method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues | |
| CN107285929A (en) | A kind of pleurotus eryngii efficient culture medium and preparation method thereof | |
| CN103082145A (en) | Method for producing grape skin residue pig feed by utilizing lentinula edodes and yeast for symbiotic fermentation | |
| CN108130295A (en) | A kind of biological organic fertilizer fermenting agent | |
| CN102488087A (en) | Biological detoxification method for camellia seed cakes | |
| TW201829775A (en) | Method of concentrating protein in grain powder | |
| CN103704476A (en) | Method for producing biological feed additive by using cassava residue and waste alcohol sediment liquid | |
| CN103652327B (en) | Biological feed produced by wastes | |
| CN104839429B (en) | The method that the combined solid fermented preparation of steam blasting is rich in fatty acid feedstuff | |
| CN102669409B (en) | Method for preparing fermentation promoting peptide of fermented feed from mushroom residue | |
| CN103719537A (en) | Nonreactive biological fermented feed and preparation method thereof | |
| CN101632411B (en) | Method for preparing high-quality rapeseed protein containing conjugated linoleic acid by multi-strain fermentation | |
| CN105124138A (en) | High-protein reed feed and preparation method thereof | |
| CN101579039B (en) | Method for producing animal feed from oil-tea-cake dregs fermented by neurospora crassa | |
| CN102389024A (en) | Method for producing feeding peanut peptide by anaerobic fermentation of peanut meal | |
| CN105111003A (en) | Liquid organic fertilizer and preparing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190315 Termination date: 20200528 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |