CN104472855A - Method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues - Google Patents
Method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues Download PDFInfo
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Abstract
The invention belongs to the technical field of a protein feed, and particularly relates to a method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues. The method comprises the following steps: firstly, adding diluted acid for pre-soaking the sweet sorghum straws and/or related waste residues, and performing steam explosion; preparing aspergillus niger and trichoderma reesei mixture by using a shallow tray solid fermentation method, and respectively culturing candida utilis and lactobacillus casei by using a liquid submerged fermentation method; spreading 0.1-1% of urea into the exposed sweet sorghum straws and/or related waste residues, inoculating the aspergillus niger and trichoderma reesei mixture and candida utilis respectively according to the inoculum sizes of 10-20% and 5-10%, evenly stirring, and performing normal-temperature aerobic fermentation for 6-8 days; inoculating lactobacillus casei to the aerobic-fermented straws according to the inoculum sizes of 3-10%, regulating the moisture content to be 60-70%, and packaging through a vacuum compression packaging machine and an anaerobic fermentation feed bag. The produced mycoprotein feed has the advantages that the content of crude protein reaches 21.5-26.1%, the content of coarse fibers are up to 6.4-8.3%, the coarse ash content reaches 2.3-4.7%, the palatability is strong, and the mycoprotein feed is easily absorbed and utilized by animals.
Description
Technical field
The invention belongs to the production technical field of protein feed, be specifically related to a kind of method utilizing sweet sorghum stalk and/or relevant waste residue to produce animal feeding-stuff containing somatic protein.
Background technology
Along with China's animal husbandry is fast-developing, feed demand amount increases greatly.But worldwide, supply falls short of demand often for the output of forage yield particularly feed albumen.At present, internal feed albumen is mainly derived from dregs of beans and fish meal, and most of dependence on import, price is also go up year by year.Further, the counter productive brought along with global range environment pollution and greenhouse effects, the output of the forage proteins such as traditional class dregs of beans and fish meal presents and reduces trend year by year.In the face of the situation of feed protein resource shortage, the substituting feed protein resource of development of new just becomes a global problem urgently to be resolved hurrily.In the exploitation of protein feed, all pay much attention to the developmental research of single-cell protein feed both at home and abroad, wherein particularly with stalk biomass, the such as research of the single cell protein of the stover of wheat, seeding corn and other crops.Sugar grass [Sorghum bicolor (L.) Moench] is a kind of excellent grain, feed, sugar material and energy class crop, its photosynthetic rate is higher, and there is the good characteristics such as drought resisting, waterlogging, Salt And Alkali Tolerance, at home and abroad started at present to promote, cultivated area expands year by year.The juice squeezed out by sweet sorghum stalk is rich in various carbohydrate, can be used as the raw materials for production of new bio energy organism ethanol; After results seed, sweet sorghum stalk also can by various preconditioning technique and solid-state fermentation technology production of cellulosic ethanol.A large amount of sugar grass waste residues can be produced while two kinds of methods produce ethanol more than use, primarily of cellulose, hemicellulose and lignin composition, its composition and sweet sorghum stalk difference to some extent.
In recent years, the new technology that sweet sorghum stalk class fodder utilizes mainly was comprised: microorganism solid fermentation method, the method for steam explosion and steam blasting-fermentable Combined Treatment, wherein respectively has advantages and disadvantages.Publication number CN 101584378 A, name is called " a kind of method taking sweet sorghum stalk as raw material and produce high protein feed ", by directly using Candida and Lactobacillus rhamnosus twice solid state fermentation sweet sorghum stalk, in final feed, total protein ratio raises, and has higher palatability.But; as everyone knows, due to the physical protection effect that the cellulose in sweet sorghum stalk, hemicellulose and lignin are wound around mutually, strongly limit microorganism to degradation efficiency coarse-fibred in stalk; therefore, simple stalk solid-fermented technique still has very large room for promotion.Publication number CN 101816370 B, name is called " agricultural crop straw that utilizes based on steam explosion technology prepares the method for high nutritive value feed ", softens by adding the chemical substances such as urea in stalk, then carries out steam blasting and use as feed.The technology simple process, but stalk urase is extremely low, low to the ammonia utilization rate in urea or ammoniacal liquor, after steam blasting, a large amount of ammonia may be formed and be discharged in air, cause certain pollution; On the other hand, although steam blasting destroys cellulosic physical arrangement to a great extent, improve the Utilization ability of animal to carbohydrate in sweet sorghum stalk, little on the content impact of total protein in stalk and true albumen.Publication number CN 102077903 A, name is called " a kind of method of steam blasting and fermentable Combined Treatment stalk ", first by stalk steam blasting, the spore liquid accessing aspergillus oryzae or koning trichoderma is again fermented, result shows: stalk after treatment cellulose equal size declines obviously, and has certain feasibility.Although above patent combines the advantage of steam blasting and fermentable two kinds of technology, but microorganism solid fermentation uses bacterial classification comparatively single, to problems such as the pentose utilization ratio in stalk are not high, feed product particularly after fermentation contains a large amount of fungal spore, and long term storage and transport also have problems.
In sum: though utilize sweet sorghum stalk to produce protein feed obtaining a lot of progress at present, technology still has the following disadvantages:
1) efficiency of existing steam blasting pretreatment sweet sorghum stalk technology awaits further raising, uses the quick-fried technology of ammonia such as urea easily to cause secondary pollution, and is unfavorable for that microorganism uses as nitrogenous source again after ammonia loss;
2) optimize fermentative microorganism strain combination, its key issue is that in stalk, pentose utilization ratio is not high, the problem such as the low and feed palatability difference of the transformation efficiency of true albumen in stalk;
3) the solution secondary fermentation production cycle is long, complex process, and fungi fermentation later stage a large amount of spore process problem.
Summary of the invention
The object of the present invention is to provide a kind of method utilizing sweet sorghum stalk and/or relevant waste residue to produce animal feeding-stuff containing somatic protein.
For achieving the above object, the technical scheme taked of the present invention is as follows:
Utilize sweet sorghum stalk and/or relevant waste residue to produce a method for animal feeding-stuff containing somatic protein, step is as follows, and following all % are mass percent:
1. pretreatment: by the sweet sorghum stalk that crushes in advance and/or relevant waste residue, adds diluted acid pre-soaking, until water content reaches 50-70% in material, then carries out steam blasting pretreatment, then naturally dry;
2. solid fermentation koji: use potato dextrose agar, conventionally respectively aspergillus niger and trichoderma reesei activation culture are reached (2.7-7.1) × 10 respectively to aspergillus niger and trichoderma reesei spore count
9individual/g, (1.8-5.3) × 10
8individual/g; Two kinds of bacterium liquid aspergillus niger in mass ratio after activation: the solid medium of the inoculative proportion access sterilizing of trichoderma reesei=1:1.5-3, inoculum concentration is that 5-10%, 25-32 DEG C of conventional tray solid fermentation cultivates 2-4 days, obtains the solid sort of quyi;
3. the liquid fermentation production of hybrid seeds: use YPD culture medium, conventionally candida utili shaking flask activation culture is reached (1.5-4.5) × 10 to viable count
8individual/g; Then access in 1# liquid seed culture medium, be placed in fermentation tank and cultivate, obtain candida utili seed liquor, wherein condition of culture: packing factor 60-70%, temperature 28-30 DEG C, inoculum concentration 1-5%, throughput 1.5-1.8m in fermentation tank
3/ h, tank pressure 0.04-0.06MPa, fermentation 36-48h; Use MRS culture medium, conventionally static for Lactobacillus casei activation culture is reached (1.8-3.8) × 10 to viable count
8individual/g; Then according in the inoculum concentration access 2# liquid seed culture medium of 7-10%, 32-36 DEG C of deep liquid anaerobic fermentation cultivates 2-4 days, obtains Lactobacillus casei seed liquor;
4. aerobic fermentation: the sweet sorghum stalk after 1. processing with step and/or relevant waste residue are for calculating basis, and be sprinkled into the urea of 0.1-1% wherein, according to the solid sort of quyi that 2. the inoculum concentration access step of 10-20% is prepared, the candida utili seed liquor that 3. step simultaneously spraying into 5-10% is prepared, after stirring, normal-temperature aerobic fermentation 6-8 days;
5. anaerobic fermentation and compression packing: Lactobacillus casei seed liquor step 3. prepared according to the inoculum concentration of 3-10% is admixed in the stalk of step 4. after aerobic fermentation, water content is adjusted to 60-70%, use vacuum compression packing machine and anaerobic fermentation feedbag packaging, skin re-uses black woven bag and packs and obtain animal feeding-stuff containing somatic protein.
Described sweet sorghum stalk is the sweet sorghum stalk in fresh sweet sorghum stalk and/or different storage period, and described relevant waste residue is the sweet sorghum stalk waste residue after squeezing the juice and/or the sweet sorghum stalk waste residue after solid state fermentation producing and ethanol.
Step 1. in, steam blasting condition is: pressure 1-4kPa, vapor (steam) temperature 200-240 DEG C, maintains pressure time 2-120s.
Step 2. in, the composition (quality %) of solid medium is: sweet sorghum stalk powder 40-60%, wheat bran 29-60%, bean cake powder 10-20%, MgSO
40.2-0.5%, KH
2pO
40.5-0.7% and water, wherein sweet sorghum stalk powder, wheat bran, bean cake powder, MgSO
4, KH
2pO
4percentage sum be 100%, solid-liquid mass ratio is 1:1.5-3.
Step 3. in, consisting of of 1# liquid seed culture medium: glucose 20-40g/L, (NH
4)
2sO
46-10g/L, KH
2pO
40.5-1g/L, MgSO
4the water of 1-3 g/L and surplus; Consisting of of 2# liquid seed culture medium: glucose 90-150 g/L, peptone 10-15 g/L, yeast extract 7.5-10 g/L, anhydrous sodium acetate 3-5 g/L, MgSO
40.2-0.4g/L, MnSO
40.05-0.07g/L, CaCO
3the water of 100-150 g/L and surplus, sterilizing, pH value 6.8.
Compared with prior art, advantage of the present invention is:
1) the present invention has fully utilized sweet sorghum stalk and relevant waste residue thereof, and for sugar grass ethanol enterprise, cost is lower has certain resources advantage;
2) for the shortcoming using steam blasting again after urea pretreated straw, the dilute acid pretreatment that working concentration of the present invention is very low combines with steam blasting, and blasting technology is optimized further, environmental friendliness, and the degradation efficiency of microorganism to stalk cellulose can be significantly improved;
3) strain combination advantage, consider making full use of of pentose and hexose in stalk, the transformation efficiency of forage protein and the raising of palatability three factors, the aerobic fermentation bacterial strain combination that the present invention uses has acid proof feature, and wherein candida utili has the ability simultaneously utilizing pentose and hexose;
4) process advantage, the present invention by the packaging of the secondary anaerobic fermentation of straw feed and feed, store and merge into a step, both the production cycle was shortened, turn improve forage protein conversion ratio and palatability, simultaneously, eliminate the techniques such as later stage drying, granulation, simplify technique, effectively can reduce the cost of Feed Manufacturing.
Accompanying drawing explanation
Fig. 1: process chart of the present invention.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described, but be not restriction the present invention.
% in following examples is quality %, and aspergillus niger, trichoderma reesei, candida utili, Lactobacillus casei are purchased from Chinese industrial Microbiological Culture Collection administrative center, and numbering is respectively: 41125,41494,31272 and 20292.
Embodiment 1
Utilize sweet sorghum stalk and/or relevant waste residue to produce a method for animal feeding-stuff containing somatic protein, as shown in Figure 1, concrete steps are as follows in its technological process:
1) pretreatment: use hay cutter by the short block of fresh sweet sorghum stalk guillotine cutting into about 0.1cm, add dilute sulfuric acid (concentration 0.1%), preimpregnation 8h, water content reaches 60%, use steam blasting device to carry out explosion, blasting condition is: temperature 210 DEG C, pressure 2kPa, maintains pressure time 90s;
2) solid fermentation koji: the aspergillus niger preserve inclined-plane and trichoderma reesei access in the triangle shaking flask containing potato dextrose agar respectively, and 28 DEG C, 150 turns/min, cultivate two days, aspergillus niger spore number reaches: 3.8 × 10
9individual/g, trichoderma reesei spore count reaches: 2.2 × 10
8individual/g; Two kinds of bacterium liquid after activation access solid fermentation culture medium according to the mass ratio of aspergillus niger and trichoderma reesei 1:1.5, and solid fermentation culture medium prescription is: sweet sorghum stalk powder (20 order) 60%, wheat bran 29.3%, bean cake powder 10%, 0.2% MgSO
4, 0.5% KH
2pO
4and water, wherein solid-liquid mass ratio 1:3, (always) inoculum concentration is 5%, routine techniques sterilizing, 28 DEG C of shallow tray fermentations 4 days, obtains the solid sort of quyi;
3) the liquid fermentation production of hybrid seeds: use YPD culture medium, conveniently technology is carried out shaking flask activation culture (temperature 28 DEG C, inoculum concentration 3%, shaking speed 120 turns/min) to candida utili and is reached to candida utili viable count: 4.5 × 10
8individual/g; Then access in 1# liquid seed culture medium, be placed in fermentation tank and cultivate, obtain candida utili seed liquor, wherein 1# liquid seed culture medium formula: glucose 20g/L, (NH
4)
2sO
46g/L, KH
2pO
40.5g/L, MgSO
4the water of 3 g/L and surplus; Candida utili condition of culture: in automatic fermenter, packing factor is 60%, temperature 28 DEG C, inoculum concentration 1%, throughput is 1.5m
3/ h, tank pressure 0.04MPa, fermentation period is 48h; Use MRS culture medium, conveniently technology sterilizing static activation culture (temperature 36 DEG C, inoculum concentration 5%) 48h is carried out to Lactobacillus casei reach to Lactobacillus casei viable count: 3.8 × 10
8individual/g, then access in 2# liquid seed culture medium, deep liquid anaerobic fermentation is cultivated, and obtains Lactobacillus casei seed liquor, wherein 2# liquid seed culture medium formula: glucose 110 g/L, peptone 10 g/L, yeast extract 10 g/L, anhydrous sodium acetate 3 g/L, MgSO
40.2 g/L, MnSO
40.05 g/L, CaCO
3the water of 100 g/L and surplus, sterilizing, pH value 6.8; Lactobacillus casei condition of culture: 36 DEG C of deep liquid anaerobic fermentations cultivate 2 days;
4) stalk aerobic fermentation: pretreated for step 1) stalk is sprinkled into the urea of 0.1%, by step 2) the solid sort of quyi prepared according to 10% inoculum concentration inoculation, candida utili seed liquor prepared by the step 3) simultaneously spraying into 5%, after stirring, normal-temperature aerobic ferments 8 days; The % of urea, candida utili seed liquor is based on the pretreated stalk of step 1);
5) anaerobic fermentation and compression packing: the Lactobacillus casei seed liquor that step 3) is prepared by the inoculum concentration according to 3% is admixed in the stalk after aerobic fermentation, water content is adjusted to 65%, use vacuum compression packing machine and anaerobic fermentation feedbag (commercially available) packaging, the outer black woven bag that uses is packed.
The nutritional labeling comparing result of animal feeding-stuff containing somatic protein prepared by the present embodiment and the fresh sweet sorghum stalk of raw material is in table 1, and nutriment assay method is all with reference to national forage standard.
Embodiment 2
Utilize sweet sorghum stalk and/or relevant waste residue to produce a method for animal feeding-stuff containing somatic protein, as shown in Figure 1, concrete steps are as follows in its technological process:
1) pretreatment: use hay cutter the natural air drying after dedusting to be stored 3 months sweet sorghum stalk guillotine cuttings short block into about 0.1cm, add dilute sulfuric acid (concentration 0.3%), preimpregnation 10h, water content reaches 65%, steam blasting device is used to carry out explosion, blasting condition is: temperature 200 DEG C, pressure 2.5kPa, maintains pressure time 100s;
2) solid fermentation koji: during activation culture, aspergillus niger spore number reaches: 2.7 × 10
9individual/g, trichoderma reesei spore count reaches: 2.6 × 10
8individual/g; The other the same as in Example 1.
3) the liquid fermentation production of hybrid seeds: during activation culture, Candida utilis ferment viable count reaches: 1.5 × 10
8individual/g, Lactobacillus casei viable count reaches: 2.8 × 10
8individual/g; The other the same as in Example 1;
4) aerobic fermentation: pretreated for step 1) stalk is sprinkled into the urea of 0.3%, by step 2) the solid sort of quyi prepared according to 15% inoculum concentration inoculation, candida utili seed liquor prepared by the step 3) simultaneously spraying into 5%, after stirring, normal-temperature aerobic ferments 8 days;
5) anaerobic fermentation and packaging: the Lactobacillus casei seed liquor that step 3) is prepared by the inoculum concentration according to 6% is admixed in the stalk after aerobic fermentation, water content is adjusted to 65%, use vacuum compression packing machine and anaerobic fermentation feedbag packaging, the outer black woven bag that uses is packed.
The nutritional labeling comparing result of animal feeding-stuff containing somatic protein prepared by the present embodiment and the air-dry sweet sorghum stalk of raw material is in table 2.
Embodiment 3
Utilize sweet sorghum stalk and/or relevant waste residue to produce a method for animal feeding-stuff containing somatic protein, as shown in Figure 1, concrete steps are as follows in its technological process:
1) pretreatment: get sweet sorghum stalk waste residue after squeezing the juice and add dilute sulfuric acid (concentration 0.7%), preimpregnation 12h, water content reaches 70%, and use steam blasting device to carry out explosion, blasting condition is: temperature 210 DEG C, pressure 3kPa, maintains pressure time 110s;
2) solid fermentation koji: during activation culture, aspergillus niger spore number reaches: 6.9 × 10
9individual/g, trichoderma reesei spore count reaches: 4.2 × 10
8individual/g; The other the same as in Example 1.
3) the liquid fermentation production of hybrid seeds: during activation culture, Candida utilis ferment viable count reaches: 2.5 × 10
8individual/g, Lactobacillus casei viable count reaches: 1.8 × 10
8individual/g; The other the same as in Example 1;
4) aerobic fermentation: pretreated for step 1) stalk waste residue is sprinkled into the urea of 0.7%, by step 2) the solid sort of quyi prepared according to 10% inoculum concentration inoculation, candida utili seed liquor prepared by the step 3) simultaneously spraying into 10%, after stirring, normal-temperature aerobic ferments 7 days;
5) anaerobic fermentation and packaging: the Lactobacillus casei seed liquor that step 3) is prepared by the inoculum concentration according to 10% is admixed in the stalk waste residue after aerobic fermentation, water content is adjusted to 65%, use vacuum compression packing machine and anaerobic fermentation feedbag packaging, the outer black woven bag that uses is packed.
Animal feeding-stuff containing somatic protein prepared by the present embodiment and raw material squeeze the juice after the nutritional labeling comparing result of sweet sorghum stalk waste residue in table 3.
Embodiment 4
Utilize sweet sorghum stalk and/or relevant waste residue to produce a method for animal feeding-stuff containing somatic protein, as shown in Figure 1, concrete steps are as follows in its technological process:
1) pretreatment: the sweet sorghum stalk waste residue after solid state fermentation producing and ethanol of learning from else's experience adds dilute sulfuric acid (concentration 1%), preimpregnation 24h, and water content reaches 68%, steam blasting device is used to carry out explosion, blasting condition is: temperature 205 DEG C, pressure 4kPa, maintains pressure time 120s;
2) solid fermentation koji: during activation culture, aspergillus niger spore number reaches: 7.1 × 10
9individual/g, trichoderma reesei spore count reaches: 5.3 × 10
8individual/g; The other the same as in Example 1.
3) the liquid fermentation production of hybrid seeds: during activation culture, Candida utilis ferment viable count reaches: 3.9 × 10
8individual/g, Lactobacillus casei viable count reaches: 3.5 × 10
8individual/g; The other the same as in Example 1;
4) aerobic fermentation: pretreated for step 1) stalk waste residue is sprinkled into the urea of 1%, by step 2) the solid sort of quyi prepared according to 20% inoculum concentration inoculation, candida utili seed liquor prepared by the step 3) simultaneously spraying into 5%, after stirring, normal-temperature aerobic ferments 9 days;
5) anaerobic fermentation and packaging: the Lactobacillus casei seed liquor that step 3) is prepared by the inoculum concentration according to 10% is admixed in the stalk waste residue after aerobic fermentation, water content is adjusted to 65%, use vacuum compression packing machine and anaerobic fermentation feedbag packaging, the outer black woven bag that uses is packed.
The nutritional labeling comparing result of animal feeding-stuff containing somatic protein prepared by the present embodiment and the raw material sweet sorghum stalk waste residue after solid state fermentation producing and ethanol is in table 4.
the animal feeding-stuff containing somatic protein prepared from embodiment 1-4 contrasts with the nutritional labeling of respective corresponding raw material: the protein feed crude protein adopting the inventive method to produce reaches 21.5-26.1%, crude fibre 6.4-8.3%, coarse ash 2.3-4.7%, palatability is strong, is easy to animal and absorbs and utilize.
Claims (5)
1. utilize sweet sorghum stalk and/or relevant waste residue to produce a method for animal feeding-stuff containing somatic protein, it is characterized in that step is as follows, and following all % be mass percent:
1. pretreatment: by the sweet sorghum stalk that crushes in advance and/or relevant waste residue, adds diluted acid pre-soaking, until water content reaches 50-70% in material, then carries out steam blasting pretreatment, then naturally dry;
2. solid fermentation koji: use potato dextrose agar, conventionally respectively aspergillus niger and trichoderma reesei activation culture are reached (2.7-7.1) × 10 respectively to aspergillus niger and trichoderma reesei spore count
9individual/g, (1.8-5.3) × 10
8individual/g; Two kinds of bacterium liquid aspergillus niger in mass ratio after activation: the solid medium of the inoculative proportion access sterilizing of trichoderma reesei=1:1.5-3, inoculum concentration is that 5-10%, 25-32 DEG C of conventional tray solid fermentation cultivates 2-4 days, obtains the solid sort of quyi;
3. the liquid fermentation production of hybrid seeds: use YPD culture medium, conventionally candida utili shaking flask activation culture is reached (1.5-4.5) × 10 to viable count
8individual/g; Then access in 1# liquid seed culture medium, be placed in fermentation tank and cultivate, obtain candida utili seed liquor, wherein condition of culture: packing factor 60-70%, temperature 28-30 DEG C, inoculum concentration 1-5%, throughput 1.5-1.8m in fermentation tank
3/ h, tank pressure 0.04-0.06MPa, fermentation 36-48h; Use MRS culture medium, conventionally static for Lactobacillus casei activation culture is reached (1.8-3.8) × 10 to viable count
8individual/g; Then according in the inoculum concentration access 2# liquid seed culture medium of 7-10%, 32-36 DEG C of deep liquid anaerobic fermentation cultivates 2-4 days, obtains Lactobacillus casei seed liquor;
4. aerobic fermentation: the sweet sorghum stalk after 1. processing with step and/or relevant waste residue are for calculating basis, and be sprinkled into the urea of 0.1-1% wherein, according to the solid sort of quyi that 2. the inoculum concentration access step of 10-20% is prepared, the candida utili seed liquor that 3. step simultaneously spraying into 5-10% is prepared, after stirring, normal-temperature aerobic fermentation 6-8 days;
5. anaerobic fermentation and compression packing: Lactobacillus casei seed liquor step 3. prepared according to the inoculum concentration of 3-10% is admixed in the stalk of step 4. after aerobic fermentation, water content is adjusted to 60-70%, use vacuum compression packing machine and anaerobic fermentation feedbag packaging, skin re-uses black woven bag and packs and obtain animal feeding-stuff containing somatic protein.
2. utilize sweet sorghum stalk and/or relevant waste residue to produce the method for animal feeding-stuff containing somatic protein as claimed in claim 1, it is characterized in that: described sweet sorghum stalk is the sweet sorghum stalk in fresh sweet sorghum stalk and/or different storage period, and described relevant waste residue is the sweet sorghum stalk waste residue after squeezing the juice and/or the sweet sorghum stalk waste residue after solid state fermentation producing and ethanol.
3. utilize sweet sorghum stalk and/or relevant waste residue to produce the method for animal feeding-stuff containing somatic protein as claimed in claim 1, in it is characterized in that step 1., steam blasting condition is: pressure 1-4kPa, vapor (steam) temperature 200-240 DEG C, maintains pressure time 2-120s.
4. utilize sweet sorghum stalk and/or relevant waste residue to produce the method for animal feeding-stuff containing somatic protein as claimed in claim 1, in it is characterized in that step is 2., consisting of of solid medium: sweet sorghum stalk powder 40-60%, wheat bran 29-60%, bean cake powder 10-20%, MgSO
40.2-0.5%, KH
2pO
40.5-0.7% and water, wherein sweet sorghum stalk powder, wheat bran, bean cake powder, MgSO
4, KH
2pO
4percentage sum be 100%, solid-liquid mass ratio is 1:1.5-3.
5. utilize sweet sorghum stalk and/or relevant waste residue to produce the method for animal feeding-stuff containing somatic protein as claimed in claim 1, in it is characterized in that step is 3., consisting of of 1# liquid seed culture medium: glucose 20-40g/L, (NH
4)
2sO
46-10g/L, KH
2pO
40.5-1g/L, MgSO
4the water of 1-3 g/L and surplus; Consisting of of 2# liquid seed culture medium: glucose 90-150 g/L, peptone 10-15 g/L, yeast extract 7.5-10 g/L, anhydrous sodium acetate 3-5 g/L, MgSO
40.2-0.4g/L, MnSO
40.05-0.07g/L, CaCO
3the water of 100-150 g/L and surplus, sterilizing, pH value 6.8.
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| CN107410701A (en) * | 2017-04-25 | 2017-12-01 | 西安鑫汉宝生物科技有限公司 | A kind of method of sweet sorghum stalk fermented feed and application thereof |
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| CN104782883A (en) * | 2015-04-10 | 2015-07-22 | 黄飚 | Method for efficient and comprehensive utilization of cornstalks |
| CN105475643A (en) * | 2015-12-25 | 2016-04-13 | 广州甘蔗糖业研究所 | Sugar cane byproduct cattle feed capable of promoting digestion |
| CN106063520A (en) * | 2016-06-22 | 2016-11-02 | 丁大喜 | A kind of preparation method of high nutritive value feedstuff for hu yang |
| CN107410701A (en) * | 2017-04-25 | 2017-12-01 | 西安鑫汉宝生物科技有限公司 | A kind of method of sweet sorghum stalk fermented feed and application thereof |
| CN107683956A (en) * | 2017-10-18 | 2018-02-13 | 沈阳金博地生态环保科技有限公司 | It is a kind of using maize peel and stalk waste residue as feed of raw material and preparation method thereof |
| CN109007257A (en) * | 2018-08-02 | 2018-12-18 | 北京首钢朗泽新能源科技有限公司 | A kind of animal feeding-stuff containing somatic protein, production technology and its application |
| CN110408553A (en) * | 2019-08-06 | 2019-11-05 | 苏州吉态来胺生物科技有限公司 | A method of single cell protein is produced by raw material of palm waste |
| CN111685233A (en) * | 2020-07-20 | 2020-09-22 | 巢湖学院 | Method for processing high-protein feed by using pear residues, pear leaves and pear branches |
| CN112931687A (en) * | 2021-03-19 | 2021-06-11 | 浙江理工大学 | Method for converting wheat straw into feed cooperatively through Fenton reaction pretreatment and inonotus obliquus solid fermentation |
| CN112913972A (en) * | 2021-03-22 | 2021-06-08 | 荆州正荣生物饲料股份有限公司 | Feed produced by utilizing steam explosion treatment green cake and method |
| CN114532444A (en) * | 2022-03-08 | 2022-05-27 | 四川农业大学 | Fibrous thallus protein feed and preparation method thereof |
| CN114874925A (en) * | 2022-06-07 | 2022-08-09 | 四川轻化工大学 | Method for producing protein feed by semi-solid fermentation of pichia kluyveri |
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