CN112931687A - Method for converting wheat straw into feed cooperatively through Fenton reaction pretreatment and inonotus obliquus solid fermentation - Google Patents
Method for converting wheat straw into feed cooperatively through Fenton reaction pretreatment and inonotus obliquus solid fermentation Download PDFInfo
- Publication number
- CN112931687A CN112931687A CN202110296604.5A CN202110296604A CN112931687A CN 112931687 A CN112931687 A CN 112931687A CN 202110296604 A CN202110296604 A CN 202110296604A CN 112931687 A CN112931687 A CN 112931687A
- Authority
- CN
- China
- Prior art keywords
- wheat straw
- fenton
- pretreatment
- feed
- inonotus obliquus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000010902 straw Substances 0.000 title claims abstract description 68
- 241000209140 Triticum Species 0.000 title claims abstract description 51
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 51
- 238000000855 fermentation Methods 0.000 title claims abstract description 42
- 230000004151 fermentation Effects 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 32
- 241000414067 Inonotus obliquus Species 0.000 title claims abstract description 25
- 239000007787 solid Substances 0.000 title claims abstract description 24
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 23
- 239000007788 liquid Substances 0.000 claims description 23
- 239000001888 Peptone Substances 0.000 claims description 22
- 108010080698 Peptones Proteins 0.000 claims description 22
- 235000019319 peptone Nutrition 0.000 claims description 22
- 229920001817 Agar Polymers 0.000 claims description 17
- 239000008272 agar Substances 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 13
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 11
- 238000011218 seed culture Methods 0.000 claims description 11
- 238000011282 treatment Methods 0.000 claims description 11
- 239000007836 KH2PO4 Substances 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 239000012028 Fenton's reagent Substances 0.000 claims description 7
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 229910052564 epsomite Inorganic materials 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 5
- 229920000053 polysorbate 80 Polymers 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 230000001131 transforming effect Effects 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 241000282849 Ruminantia Species 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 229920005610 lignin Polymers 0.000 description 14
- 239000001913 cellulose Substances 0.000 description 13
- 229920002678 cellulose Polymers 0.000 description 13
- 239000000047 product Substances 0.000 description 11
- 229920002488 Hemicellulose Polymers 0.000 description 7
- 230000009286 beneficial effect Effects 0.000 description 7
- 230000015556 catabolic process Effects 0.000 description 7
- 238000006731 degradation reaction Methods 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 235000019750 Crude protein Nutrition 0.000 description 5
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 238000002203 pretreatment Methods 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000007259 addition reaction Methods 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000012840 feeding operation Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- -1 iron ions Chemical class 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Birds (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for converting wheat straw into feed by cooperation of Fenton reaction pretreatment and inonotus obliquus solid fermentation, which takes agricultural resource waste wheat straw as a raw material and adopts the Fenton reaction pretreatment and inonotus obliquus solid fermentation methods to prepare feed protein. The wheat straw feed protein prepared by the invention can effectively improve the production performance and feed conversion rate of ruminants, and has good market prospect.
Description
Technical Field
The invention belongs to the technical field of fermentation engineering, and particularly relates to a method for converting wheat straw into a feed by combining Fenton reaction pretreatment and inonotus obliquus solid fermentation.
Background
In recent years, the best way for treating crop straws by biological means is always searched at home and abroad, and the problem has the greatest difficulty that water-insoluble lignocellulose in the straws is difficult to degrade by acid, alkali and enzyme, mainly caused by the crystallinity and the polymerization degree of the cellulose and lignin wrapping the cellulose and hemicellulose. Lignin and hemicellulose are combined in a covalent bond mode, cellulose molecules are embedded in the lignin and the hemicellulose to form a natural barrier, so that enzymes are not easily contacted with the cellulose molecules, and the water insolubility and the chemical structure complexity of the lignin cause the difficult degradation of straws. Therefore, to completely degrade cellulose, the problem of lignin degradation must be solved.
Some common pretreatment methods of crop straws include physical treatment, chemical treatment and the like. Physical treatments such as grinding, soaking, granulating, etc., all of which reduce the crystallinity of the cellulose, ultimately resulting in better degradation of the cellulose by hydrolytic enzymes. However, the physical treatment method requires machinery and processing procedures, so that the application in agriculture is limited, and although the reduction of the particle size is really beneficial to the subsequent enzyme treatment process, the crushed crop straws are not beneficial to the later-stage feeding operation. Although the acid-base treatment method can reduce the crystallinity of cellulose and increase the yield of saccharides, the application of sulfuric acid and sodium hydroxide is not only expensive, but also causes serious environmental pollution. Meanwhile, the method does not have the function of increasing the protein content, and the single microbial solid fermentation method cannot obviously increase the protein content.
The Fenton reaction is characterized in that a chain reaction between divalent iron ions (Fe2+) and hydrogen peroxide catalyzes a hydroxyl radical to generate stronger oxidation capacity, the oxidation potential of the hydroxyl radical is second to that of fluorine, in addition, the hydroxyl radical has high electronegativity or electrophilicity, the electron affinity of the hydroxyl radical is as high as 569.3kJ, and the hydroxyl radical has strong addition reaction characteristics, so that the Fenton reagent can indiscriminately oxidize most organic matters in water, and is particularly suitable for oxidation treatment of organic wastewater which is difficult to biodegrade or generally difficult to effectively oxidize chemically.
Disclosure of Invention
The invention aims to provide a method for converting wheat straw into a feed by combining Fenton reaction pretreatment and inonotus obliquus solid fermentation, solves the problems of low crude protein content and high lignin content in the process of using the wheat straw as the feed, and improves the nutritional value of the wheat straw as the feed.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a method for transforming wheat straw into feed by cooperation of Fenton reaction pretreatment and inonotus obliquus solid fermentation comprises the following steps:
(1) preparing a seed solution: activating the inonotus obliquus strain in a malt peptone agar culture medium, and transferring to a liquid seed culture medium for culture to obtain a seed solution;
(2) straw Fenton pretreatment: cutting the wheat straws to wheat straw sections with the length of 1.0-1.5cm, carrying out Fenton pretreatment, washing cleanly after the treatment is finished, and airing;
(3) solid fermentation: and (3) uniformly mixing the wheat straws treated in the step (2) with the nutrient solution, sterilizing, inoculating seed solution, fermenting for 25-30 days at the humidity of 75 +/-3% and the temperature of 26-28 ℃, taking out a fermentation product after the fermentation is finished, and drying to obtain the product.
The wheat straw is cut to 1.0-1.5cm in length to facilitate degradation, and if the wheat straw is ground into powder, liquid is added subsequently to be mixed, so that the wheat straw is not beneficial to strain culture; meanwhile, the water is soaked to ensure that the water fully permeates the matrix, which is also beneficial to the degradation of the inner part of the wheat straw by the strains; the air drying of the wheat straws is that the inonotus obliquus can not be cultured on a substrate with too large water content, so that the wheat straws are air-dried until no water obviously flows out.
The Fenton pretreatment has mild reaction conditions, low cost and environmental friendliness, can reduce the molecular weight of lignin and promote the enzymatic saccharification of lignocellulose biomass, and meanwhile, the treated wheat straw has no reagent residue, so that the method is beneficial to the solid fermentation of the inonotus obliquus at the later stage, and the protein content is improved.
The Fenton reaction can not change or improve the nutritive value of crop straws, but can destroy the lignin structure, promote the degradation capability of the inonotus obliquus on lignocellulose and further improve the protein production capability of white rot fungi. The invention adopts the Fenton reaction pretreatment and the inonotus obliquus solid fermentation synergistic treatment, can obviously increase the protein content of the fermentation product, simultaneously reduces the lignin content, and improves the nutritional value of the wheat straw as the feed.
On one hand, the Fenton reagent adopted by the invention can randomly attack cell walls due to high-activity hydroxyl free radicals (. OH) generated by the Fenton reagent, so that the lignocellulose structure is damaged, the treated wheat straw is beneficial to the growth and degradation of the inonotus obliquus, so that the protein content in the wheat straw is improved, meanwhile, the treated wheat straw is more crisp and fragile, and the palatability of the straw feed is improved; on the other hand, the Fenton reaction has low cost, low consumption, mild reaction and environmental protection, and saves a large amount of resources. Based on the two aspects, the straw wheat straw which is pretreated by Fenton and subjected to solid fermentation by the inonotus obliquus is used as the feed of the ruminant, so that a new way is provided for deep utilization of the wheat straw resource.
The straw pretreated by Fenton in the invention is crisp and fragile, and lignin, cellulose and hemicellulose can be decomposed and utilized when the straw is subjected to solid fermentation by inonotus obliquus, and a large amount of mycoprotein is generated, so that the protein content and the nutritional quality of the straw converted feed are greatly improved. The protein content is detected to be increased from 6.45 percent to 20.59 percent, so the feed is suitable for feeding ruminants. The invention has simple fermentation process, low cost, good flavor of the fermented product, and improved crude protein content to different degrees, thereby achieving the effect of producing high-quality fermented feed.
Preferably, in step (2), the Fenton reagent for Fenton pretreatment is prepared from FeSO4Solution and H2O2Composition of, wherein FeSO4Final concentration of 0.015-0.025mol/L, H2O2The final concentration is 1.5-2.5 mol/L.
Preferably, in the step (2), the Fenton pretreatment reaction parameters are as follows: the solid-liquid ratio of the wheat straw section to the Fenton reagent is 1g:15-18mL, the temperature is 50 +/-2 ℃, the rotating speed of a shaking table is 200 +/-10 r/min, and the time is 2.5-3.5 h.
Preferably, in the step (3), the formula of the nutrient solution is as follows: corn flour 200g/L, (NH)4)2SO4 10g/L，MgSO4·7H2O 0.3g/L，KH2PO4 0.11g/L，Tween-80 4g/L，pH 5.0。
Preferably, in the step (3), the solid-to-liquid ratio of the wheat straws to the nutrient solution is 1g:2-3mL, and the dosage of the seed solution is 0.5-1.0mL per 1g of wheat straws.
Preferably, in the step (1), the liquid seed culture medium formula is as follows: 20g/L glucose, 3g/L peptone, 2g/L yeast extract, KH2PO4 1g/L，MgSO4 1.5g/L，CaCl20.1g/L, pH is natural.
Preferably, in the step (1), the parameters of the liquid seed culture medium culture are as follows: the temperature is 28 +/-1 ℃, the rotating speed is 150 +/-10 r/min, and the culture time is 48 +/-1 h.
Preferably, in the step (1), the malt peptone agar medium formula is as follows: 30g/L of malt extract powder, 3g/L of peptone, 15g/L of agar and 5.4-5.8 of pH.
Preferably, in step (1), the parameters of activation in malt peptone agar medium are: the temperature is 28 +/-1 ℃, and the time is 72 +/-1 h.
The invention has the beneficial effects that: the invention takes agricultural resource waste wheat straws as raw materials, adopts Fenton reaction pretreatment and inonotus obliquus solid fermentation methods to prepare the feed protein, provides a safe, low-cost and environment-friendly pretreatment method, can change waste into valuable, and provides a new high-quality feed protein with safety and high nutritional value.
The wheat straw feed protein prepared by the invention can effectively improve the production performance and feed conversion rate of ruminants, and has good market prospect.
Drawings
FIG. 1 is the change in cellulose content during fermentation.
FIG. 2 is the change in hemicellulose content during fermentation.
FIG. 3 is the change in lignin content during fermentation.
Detailed Description
The technical solution of the present invention will be further specifically described below by way of specific examples.
In the present invention, the raw materials and equipment used are commercially available or commonly used in the art, unless otherwise specified. The methods in the following examples are conventional in the art unless otherwise specified.
Example 1:
a method for transforming wheat straw into feed by cooperation of Fenton reaction pretreatment and inonotus obliquus solid fermentation comprises the following steps:
(1) preparing a seed solution: the inonotus obliquus strain (commercially available) is firstly activated in a malt peptone agar medium, and the activation parameters are as follows: the temperature is 27 ℃, and the time is 73 h; then transferring to a liquid seed culture medium for culture, wherein the culture parameters are as follows: the temperature is 27 ℃, the rotating speed is 160r/min, and the culture time is 49h, thus obtaining the seed liquid.
The malt peptone agar medium formula is: 30g/L of malt extract powder, 3g/L of peptone, 15g/L of agar and 5.4-5.8 of pH.
The formula of the liquid seed culture medium is as follows: 20g/L glucose, 3g/L peptone, 2g/L yeast extract, KH2PO4 1g/L，MgSO4 1.5g/L，CaCl20.1g/L, pH is natural.
(2) Pretreatment of wheat straws: cutting wheat straw to 1cm length, and adding equal volume of 0.015mol/L FeSO according to the solid-liquid ratio of 1:164The solution was mixed with 1.5mol/L of H2O2Taking out the mixture after 2.5 hours at the temperature of 52 ℃ and the rotating speed of 210r/min, and cleaning and airing the mixture; (3) solid fermentation: and (3) uniformly mixing 3g of wheat straw treated in the step (2) with 9mL of nutrient solution, sterilizing at 121 ℃ for 30min, inoculating 1.5mL of seed solution, fermenting at the humidity of 72% and the temperature of 27 ℃ for 30 days, taking out a fermentation product after the fermentation is finished, and then drying in an oven at 55 ℃ for 1 day to obtain the product.
The nutrient solution formula is as follows: corn flour 200g/L, (NH)4)2SO4 10g/L，MgSO4·7H2O 0.3g/L，KH2PO40.11g/L，Tween-80 4g/L，pH 5.0。
Example 2:
a method for transforming wheat straw into feed by cooperation of Fenton reaction pretreatment and inonotus obliquus solid fermentation comprises the following steps:
(1) preparing a seed solution: the inonotus obliquus strain (commercially available) is firstly activated in a malt peptone agar medium, and the activation parameters are as follows: the temperature is 29 ℃, and the time is 71 h; then transferring to a liquid seed culture medium for culture, wherein the culture parameters are as follows: the temperature is 29 ℃, the rotating speed is 140r/min, and the culture time is 47h, thus obtaining the seed liquid.
The malt peptone agar medium formula is: 30g/L of malt extract powder, 3g/L of peptone, 15g/L of agar and 5.4-5.8 of pH.
The formula of the liquid seed culture medium is as follows: 20g/L glucose, 3g/L peptone, 2g/L yeast extract, KH2PO4 1g/L，MgSO4 1.5g/L，CaCl20.1g/L, pH is natural.
(2) Pretreatment of wheat straws: cutting wheat straw to 1.2cm length, adding equal volume of 0.025mol/L FeSO according to solid-liquid ratio of 1:184The solution was mixed with 2.5mol/L H2O2Taking out, cleaning and airing at the temperature of 48 ℃ and the rotating speed of 190r/min for 3.5 hours; (3) solid fermentation: and (3) uniformly mixing 3g of wheat straw treated in the step (2) with 7mL of nutrient solution, sterilizing at 121 ℃ for 30min, inoculating 3mL of seed solution, fermenting at the humidity of 78% and the temperature of 28 ℃ for 25 days, taking out a fermentation product after the fermentation is finished, and then drying in an oven at 55 ℃ for 1 day to obtain the product.
The nutrient solution formula is as follows: corn flour 200g/L, (NH)4)2SO4 10g/L，MgSO4·7H2O 0.3g/L，KH2PO40.11g/L，Tween-80 4g/L，pH 5.0。
Example 3:
a method for transforming wheat straw into feed by cooperation of Fenton reaction pretreatment and inonotus obliquus solid fermentation comprises the following steps:
(1) preparing a seed solution: the inonotus obliquus strain (commercially available) is firstly activated in a malt peptone agar medium, and the activation parameters are as follows: the temperature is 28 ℃, and the time is 72 h; then transferring to a liquid seed culture medium for culture, wherein the culture parameters are as follows: the temperature is 28 ℃, the rotating speed is 150r/min, and the culture time is 48h, thus obtaining the seed liquid.
The malt peptone agar medium formula is: 30g/L of malt extract powder, 3g/L of peptone, 15g/L of agar and 5.4-5.8 of pH.
Liquid, method for producing the same and use thereofThe formula of the seed culture medium is as follows: 20g/L glucose, 3g/L peptone, 2g/L yeast extract, KH2PO4 1g/L，MgSO4 1.5g/L，CaCl20.1g/L, pH is natural.
(2) Pretreatment of wheat straws: cutting wheat straw to 1.5cm length, and adding equal volume of 0.02mol/L FeSO according to the solid-to-liquid ratio of 1:154The solution was mixed with 2.0mol/L H2O2Taking out, cleaning and airing at the temperature of 50 ℃ and the rotating speed of 200r/min after 3 hours; (3) solid fermentation: and (3) uniformly mixing 3g of wheat straw treated in the step (2) with 6mL of nutrient solution, sterilizing at 121 ℃ for 30min, inoculating 2mL of seed solution, fermenting at the humidity of 75% and the temperature of 26 ℃ for 30 days, taking out a fermentation product after the fermentation is finished, and then drying in an oven at 55 ℃ for 1 day to obtain the product.
The nutrient solution formula is as follows: corn flour 200g/L, (NH)4)2SO4 10g/L，MgSO4·7H2O 0.3g/L，KH2PO40.11g/L，Tween-80 4g/L，pH 5.0。
The product of example 3 was tested to have a mycelium content of 149.91g/kg (Table 1), a crude protein content of 20.6%, an increase of 221.9% (Table 2), a cellulose content of 39.9% (FIG. 1), a hemicellulose content of 15.4% (FIG. 2), and a lignin content of 18.3% (FIG. 3). The amount of mycelia produced from wheat straws which were not treated by the method of the present invention was 139.3g/kg (Table 1), the crude protein content was 6.7%, the increase was 4.7% (Table 2), the cellulose content was 33.79% (FIG. 1), the hemicellulose content was 23.16% (FIG. 2), and the lignin content was 22.23% (FIG. 3). Although the reduced protein content (4.5%) of straw after Fenton pretreatment (Table 2) was observed, it has a significant positive effect on the subsequent solid fermentation of Inonotus obliquus.
TABLE 1 variation of mycelium amount during fermentation (g mycelium/kg straw)
| Time (sky) | Without Fenton | Fenton treatment | |
| 5 | 54.45 | 57.98 | |
| 10 | 68.6 | 82.73 | |
| 15 | 82.73 | 111.02 | |
| 20 | 96.87 | 128.69 | |
| 20 | 125.16 | 139.3 | |
| 30 | 139.3 | 149.91 |
TABLE 2 Change in protein content during fermentation
Therefore, the straw pretreated by Fenton is subjected to solid fermentation, lignin can be degraded under the condition of maximally preserving the cellulose content, the crude protein content of the straw can be obviously increased, and the method is a relatively excellent pretreatment method.
The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit of the invention as set forth in the claims.
Claims (9)
1. A method for transforming wheat straw into feed by cooperation of Fenton reaction pretreatment and inonotus obliquus solid fermentation is characterized by comprising the following steps:
(1) preparing a seed solution: activating the inonotus obliquus strain in a malt peptone agar culture medium, and transferring to a liquid seed culture medium for culture to obtain a seed solution;
(2) straw Fenton pretreatment: cutting the wheat straws to wheat straw sections with the length of 1.0-1.5cm, carrying out Fenton pretreatment, washing cleanly after the treatment is finished, and airing;
(3) solid fermentation: and (3) uniformly mixing the wheat straws treated in the step (2) with the nutrient solution, sterilizing, inoculating seed solution, fermenting for 25-30 days at the humidity of 75 +/-3% and the temperature of 26-28 ℃, taking out a fermentation product after the fermentation is finished, and drying to obtain the product.
2. The method of claim 1, wherein in step (2), the Fenton reagent for Fenton pretreatment is prepared from FeSO4Solution and H2O2Composition of, wherein FeSO4Final concentration of 0.015-0.025mol/L, H2O2The final concentration is 1.5-2.5 mol/L.
3. The method according to claim 1 or 2, wherein in the step (2), the Fenton pretreatment reaction parameters are as follows: the solid-liquid ratio of the wheat straw section to the Fenton reagent is 1g:15-18mL, the temperature is 50 +/-2 ℃, the rotating speed of a shaking table is 200 +/-10 r/min, and the time is 2.5-3.5 h.
4. The method of claim 1, wherein the method is performed in a batch modeCharacterized in that in the step (3), the formula of the nutrient solution is as follows: corn flour 200g/L, (NH)4)2SO4 10g/L，MgSO4·7H2O 0.3g/L，KH2PO4 0.11g/L，Tween-80 4g/L，pH 5.0。
5. The method according to claim 1 or 4, wherein in the step (3), the solid-to-liquid ratio of the wheat straw to the nutrient solution is 1g:2-3mL, and the amount of the seed solution is 0.5-1.0mL per 1g of the wheat straw.
6. The method of claim 1, wherein in step (1), the liquid seed medium is formulated as: 20g/L glucose, 3g/L peptone, 2g/L yeast extract, KH2PO4 1g/L，MgSO4 1.5g/L，CaCl20.1g/L, pH is natural.
7. The method according to claim 1 or 6, wherein in step (1), the parameters of the liquid seed culture medium culture are: the temperature is 28 +/-1 ℃, the rotating speed is 150 +/-10 r/min, and the culture time is 48 +/-1 h.
8. The method according to claim 1, wherein in step (1), the malt peptone agar medium is formulated as: 30g/L of malt extract powder, 3g/L of peptone, 15g/L of agar and 5.4-5.8 of pH.
9. The method according to claim 1 or 8, wherein in step (1), the parameters of activation in malt peptone agar medium are: the temperature is 28 +/-1 ℃, and the time is 72 +/-1 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110296604.5A CN112931687A (en) | 2021-03-19 | 2021-03-19 | Method for converting wheat straw into feed cooperatively through Fenton reaction pretreatment and inonotus obliquus solid fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110296604.5A CN112931687A (en) | 2021-03-19 | 2021-03-19 | Method for converting wheat straw into feed cooperatively through Fenton reaction pretreatment and inonotus obliquus solid fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112931687A true CN112931687A (en) | 2021-06-11 |
Family
ID=76226936
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110296604.5A Pending CN112931687A (en) | 2021-03-19 | 2021-03-19 | Method for converting wheat straw into feed cooperatively through Fenton reaction pretreatment and inonotus obliquus solid fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112931687A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101715872A (en) * | 2009-11-24 | 2010-06-02 | 陕西省科学院酶工程研究所 | Method for preparing high-protein sacchariferous feed by straws |
| CN104472855A (en) * | 2014-11-21 | 2015-04-01 | 河南农业大学 | Method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues |
| CN105918615A (en) * | 2016-04-25 | 2016-09-07 | 江苏大学 | Production method of large-scale rice wheat straw microorganism feed |
| CN107029791A (en) * | 2017-04-25 | 2017-08-11 | 河南农业大学 | A kind of fenton catalyst and preparation method thereof, the application in lignocellulosic pretreatment |
| CN107119094A (en) * | 2017-06-16 | 2017-09-01 | 中南大学 | A kind of method of utilization lignin-degrading bacteria reinforcing abandoned biomass Fenton reaction pretreatments |
| CN107535675A (en) * | 2017-09-07 | 2018-01-05 | 浙江理工大学 | A kind of Inonotus obliquus solid fermentation method of straw feed conversion |
| CN108902446A (en) * | 2018-06-20 | 2018-11-30 | 十堰真武酒业有限公司 | A kind of high protein lees feeds and preparation method thereof |
-
2021
- 2021-03-19 CN CN202110296604.5A patent/CN112931687A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101715872A (en) * | 2009-11-24 | 2010-06-02 | 陕西省科学院酶工程研究所 | Method for preparing high-protein sacchariferous feed by straws |
| CN104472855A (en) * | 2014-11-21 | 2015-04-01 | 河南农业大学 | Method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues |
| CN105918615A (en) * | 2016-04-25 | 2016-09-07 | 江苏大学 | Production method of large-scale rice wheat straw microorganism feed |
| CN107029791A (en) * | 2017-04-25 | 2017-08-11 | 河南农业大学 | A kind of fenton catalyst and preparation method thereof, the application in lignocellulosic pretreatment |
| CN107119094A (en) * | 2017-06-16 | 2017-09-01 | 中南大学 | A kind of method of utilization lignin-degrading bacteria reinforcing abandoned biomass Fenton reaction pretreatments |
| CN107535675A (en) * | 2017-09-07 | 2018-01-05 | 浙江理工大学 | A kind of Inonotus obliquus solid fermentation method of straw feed conversion |
| CN108902446A (en) * | 2018-06-20 | 2018-11-30 | 十堰真武酒业有限公司 | A kind of high protein lees feeds and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Villas-Boˆas et al. | Bioconversion of apple pomace into a nutritionally enriched substrate by Candida utilis and Pleurotus ostreatus | |
| CN105795098B (en) | Cassava residue feed and preparation method thereof | |
| CN104256086B (en) | Technology for preparing docosahexaenoic acid (DHA)-rich feed additive by grain dreg raw material through fermentation | |
| CN104824337A (en) | Preparation method of fermented soybean meal for feeding | |
| CN110384177B (en) | Method for preparing yeast culture based on distiller's grains and application of yeast culture in animal feed | |
| CN100497552C (en) | Process for preparing fuel ethanol by using straw fiber materials | |
| CN114940960B (en) | Composite microbial preparation for degrading kitchen waste, preparation method and application thereof | |
| CN114875105A (en) | Biomass-based efficient carbon source and preparation method thereof | |
| CN102860413A (en) | Nutritive feed and preparation method thereof | |
| CN114190475B (en) | Straw fermented feed and production method thereof | |
| CN115011648B (en) | Fermentation method for enhancing corn straw sugar production by clostridium thermocellum | |
| CN112931687A (en) | Method for converting wheat straw into feed cooperatively through Fenton reaction pretreatment and inonotus obliquus solid fermentation | |
| CN107535675B (en) | Inonotus obliquus solid fermentation method for wheat straw conversion feed | |
| CN115197983A (en) | Beta 1,3 glucan and preparation method thereof | |
| Umasaravanan et al. | Comparison of lignocellulose biodegradation in solid state fermentation of sugarcane bagasse and rice straw by aspergillus tamatii | |
| CN110583867B (en) | Method for producing protein feed from waste potato residues in potato starch processing | |
| CN113151218A (en) | Production method and application of heat-resistant xylanase | |
| CN114574527A (en) | Fermentation method for optimizing hydrolyzed feather meal | |
| CN109762769B (en) | Anaerobic straw degradation microbial inoculum and preparation method and application thereof | |
| Tian et al. | A newly isolated Cerrena unicolor capable of laccase production and lignin degradation in agricultural wastes | |
| CN108813105B (en) | Method for wrapping corn cobs and corncobs for ensiling | |
| CN109511949A (en) | A method of using vinegar lees for nutritional additive | |
| CN116590189B (en) | Microbial fermentation inoculant for corn stalks and preparation method thereof | |
| KR101408972B1 (en) | Method of producing bio ethanol using the composition for accelerating fermentation | |
| CN1468828A (en) | Organic fertilizer and its production process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210611 |