CN104839382A - Lucid ganoderma and Chinese flowering crabapple tea as well as preparation process and application thereof - Google Patents
Lucid ganoderma and Chinese flowering crabapple tea as well as preparation process and application thereof Download PDFInfo
- Publication number
- CN104839382A CN104839382A CN201410279484.8A CN201410279484A CN104839382A CN 104839382 A CN104839382 A CN 104839382A CN 201410279484 A CN201410279484 A CN 201410279484A CN 104839382 A CN104839382 A CN 104839382A
- Authority
- CN
- China
- Prior art keywords
- tea
- chinese flowering
- glossy ganoderma
- flowering crabapple
- malus spectabilis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a lucid ganoderma and Chinese flowering crabapple tea and a preparation method thereof. The lucid ganoderma and Chinese flowering crabapple tea is a lucid ganoderma powder and Chinese flowering crabapple tea bag or a lucid ganoderma spore powder and Chinese flowering crabapple tea bag or a fermented lucid ganoderma and Chinese flowering crabapple tea bag. The lucid ganoderma and Chinese flowering crabapple tea is a health-care product. The Chinese flowering crabapple tea is applied in treating or preventing in vitro hepatocyte damage, chronic alcoholic hepatocyte damage and breast cancer as well as reducing blood fat and blood glucose. The preparation process is simple, materials are easily obtained, and the application in health-care tea is considerable. The health-care tea has a good synergistic effect in CCL4-induced hepatocyte damage. In addition, the health-care tea has an obvious inhibiting effect on serum ALT (alanine aminotransferase) in mouse with chronic alcoholic hepatocyte damage, and shows a good synergistic effect. The lucid ganoderma and Chinese flowering crabapple tea can be widely promoted.
Description
Technical field
The present invention relates to a kind of glossy ganoderma Chinese flowering crabapple tea, and preparation method thereof, and it can be used as health protection tea to be applied to preparation treatment or prevent, in hepatocellular injury and breast cancer, to be applied to simultaneously and to reduce on blood pressure and blood lipoid.
Background technology
Hubei Chinese flowering crabapple (Malus hupehensis (Pamp.) Rehd.) is rose family Malus medicine food dual purpose plant, Hubei Chinese flowering crabapple is drunk with a long history as tealeaves, it is the cold drink that Sanxia area widely drinks summer, Hubei Chinese flowering crabapple leaf is rich in the materials such as the Flavonoid substances based on phloridzin, we study find its have protect the liver, estrogen-like action and the multiple effect such as antiviral.
Glossy ganoderma Ganoderma lucidum (Leyss.ex Fr.) Karst., also known as auspicious grass, polyporus lucidus, is Ganoderma medicine-food two-purpose fungi.Lucidum spore powder is new food in a steady stream.Glossy ganoderma is distributed in all over the world, is many with subtropical and tropical zones.In China glossy ganoderma history of medicinal existing more than 2000 year, its fructification, conidia powder, mycelium all can be used as medicine.In Shennong's Herbal, recite effect of several glossy ganoderma such as red sesame, black sesame, Bai Zhi, Huang Zhi, purple sesame.A large amount of pharmacological research shows, glossy ganoderma has immunity moderation, protects the liver, antitumor, anti-ageing, improve body's hypoxia tolerance isoreactivity.
But Hubei Chinese flowering crabapple is mixed obtained health protection tea with glossy ganoderma and the relevant healthcare function of this tea is not reported so far.
Summary of the invention
The object of the present invention is to provide and a kind of obtain glossy ganoderma Chinese flowering crabapple tea with Hubei Chinese flowering crabapple, glossy ganoderma for base stock.
Be specially:
A kind of glossy ganoderma Chinese flowering crabapple tea, this glossy ganoderma Chinese flowering crabapple tea is glossy ganoderma powder Malus spectabilis tea in bag.
Its preparation technology is:
Get ganoderma lucidum fruitbody section, separately get Hubei Chinese flowering crabapple tealeaves respectively at the baking oven inner drying 20-40min being preheated to 60-80 DEG C, control moisture below 8%, pulverize the section of dried ganoderma lucidum fruitbody and dried Hubei Hubei Chinese flowering crabapple tealeaves respectively, and cross 20-60 order; By weight, after 10 parts ~ 200 portions glossy ganodermas are mixed with 80 parts ~ 120 Hubei Chinese flowering crabapples, again pulverize and sieve, and cross 80-120 order, pack often to wrap 0.2-5g.
A kind of glossy ganoderma Hubei Chinese flowering crabapple tea, this glossy ganoderma Hubei Chinese flowering crabapple tea is glossy ganoderma powder Hubei Chinese flowering crabapple tea in bag.
Its preparation technology is:
Get ganoderma lucidium spore powder wall breaking, separately get Hubei Chinese flowering crabapple tealeaves respectively at the baking oven inner drying 20-40min being preheated to 60-80 DEG C, control moisture below 8%, pulverize dried lucidum spore powder and dried Hubei Chinese flowering crabapple tealeaves respectively, and cross 20-60 order; By weight, after 10 parts ~ 200 parts lucidum spore powders are mixed with 80 parts ~ 120 Hubei Chinese flowering crabapples, again pulverize and sieve, and cross 80-120 order, pack often to wrap 0.2-5g.
A kind of glossy ganoderma Hubei Chinese flowering crabapple tea, this glossy ganoderma Hubei Chinese flowering crabapple tea is glossy ganoderma fermentation Hubei Chinese flowering crabapple tea in bag
Its preparation technology is:
Make potato culture medium: potato 200g, glucose 20g, agar 20g, water 1000ml, be cut into small pieces potato and put into pot, and add water 1000ml, boil 20 minutes, by filtered through gauze, moisturizing, to 1000ml, puts into agar and glucose, heat after dissolving, adjust pH to 6.0 with potassium dihydrogen phosphate or dipotassium hydrogen phosphate solution, by culture medium packing conical flask, often bottled enter 1/3 capacity culture medium, 0.1-0.15MPa steam high-voltage sterilizing 30min, obtained potato culture medium;
Actication of culture: take out lucidum strain from strain library, inoculates on aseptic operating platform, uses shaking table shaken cultivation, rotating speed 150r/min, temperature 25 DEG C, cultivates 5-7 days, the bacterial classification of obtained activation;
Make seed culture fluid: glucose 20g, gypsum 2g, analysis for soybean powder 10g, adjust pH to 6.0 with potassium dihydrogen phosphate or dipotassium hydrogen phosphate solution after malt flour 2g, 20% potato juice 1000ml prepare, be sub-packed in the triangular flask of 500ml, every bottled 150ml, autoclaving, inoculates the bacterial classification 5-10ml of activation on aseptic operating platform, use shaking table shaken cultivation, rotating speed 120r/min, temperature 25 DEG C, cultivates 5-7 days;
Inoculation and cultivation: Hubei Chinese flowering crabapple tealeaves is loaded in culture vessel, add the water infiltration of 0.6-1.5 times of Hubei Chinese flowering crabapple tealeaves weight, sterilizing 20-30min at 121 DEG C, after taking out culture vessel, aseptically inoculate seed liquor 5-10ml, cultivate 10-30 days for 20-30 DEG C, at 121 DEG C, sterilizing 30min;
The preparation of tea in bag: the Hubei Chinese flowering crabapple fermented tea after sterilizing, at the baking oven inner drying 20-40min being preheated to 60-80 DEG C, controls moisture below 8%, pulverizes dried glossy ganoderma and dried Hubei Chinese flowering crabapple fermented tea respectively, and cross 20-60 order; By weight, after 10 parts ~ 200 parts glossy ganoderma powder are mixed with 80 parts ~ 120 Hubei Chinese flowering crabapples, again pulverize and sieve, and cross 80-120 order, pack often to wrap 0.2-5g.
Glossy ganoderma Chinese flowering crabapple tea obtained by the present invention is applied on the health protection tea of preparation treatment or prevention in vitro liver cell damage.
Glossy ganoderma Chinese flowering crabapple tea obtained by the present invention is applied on the health protection tea of preparation treatment or pre-chronic alcoholic hepatocellular injury.
Glossy ganoderma Chinese flowering crabapple tea obtained by the present invention is applied on the health protection tea of preparation treatment or prevention breast cancer.
The invention has the advantages that:
Preparation technology of the present invention is simple, and material is easy to get, and the application on health protection tea is considerable.This health protection tea is at CCL
4the hepatocellular injury of induction has good synergy.This health protection tea to the inhibitory action of ALT in chronic alcohol liver injury mice serum also clearly, shows good synergy simultaneously.
Detailed description of the invention
Embodiment 1
Get ganoderma lucidum fruitbody section, separately get Malus spectabilis tealeaves respectively at the baking oven inner drying 20-40min being preheated to 60-80 DEG C, control moisture below 8%, pulverize the section of dried ganoderma lucidum fruitbody and dried Malus spectabilis tealeaves respectively, and cross 20-60 order; By weight, after 10 parts ~ 200 portions glossy ganodermas are mixed with 80 parts ~ 120 Malus spectabilis, again pulverize and sieve, and cross 80-120 order, pack often to wrap 0.2-5g.
Glossy ganoderma and Malus spectabilis water extract are to CCL
4the synergistic protective effect of inducing hepatocyte damage:
Get about 10g kunming mice 1, sacrificed by decapitation, aseptic separation liver, weighs in rearmounted PBS liquid, irrigation liver is to canescence, liver is cut into the tissue block of about 1mm3, and mills with nylon screen, add PBS liquid, the centrifugal 5min of 1000r/min, remove supernatant, add 10ml nutrient solution, break into hepatocyte suspension with dropper featheriness.Get the above-mentioned hepatocyte suspension of 50ul to forward in 96 orifice plates, if CCL
4damage model group, various dose administration group, Normal group, often group establishes 6 multiple holes.Model group and administration group add 4mmol.L-1CCL
4, simultaneously administration group individually with combine the sample adding variable concentrations, glossy ganoderma water extract, the concentration of ganoderma spove powder water extract (particle by preparation technology's processing processed of instant tea) is 10 μ g/mL, the concentration of crab apple extract is 5 μ g/mL, 10 μ g/mL, 20 μ g/mL and 50 μ g/mL, the concentration that glossy ganoderma Malus spectabilis instant tea is equivalent to containing glossy ganoderma water extract or ganoderma spove powder water extract is 10 μ g/mL, the concentration simultaneously containing crab apple extract is respectively 5 μ g/mL, 10 μ g/mL, 20 μ g/mL and 50 μ g/mL, 37 DEG C hatch 3 hours after, the centrifugal 5min of 2000r/min, collect culture supernatant and detect ALT activity.Calculate the inhibiting rate of each experimental group to ALT, and calculate drug combination index (CI) with CompuSyn software, as CI < 1, show that both have synergy; As CI > 1, then show that both have antagonism; As CI=1, show that both have summation action.Result is as follows:
Table 1 glossy ganoderma and Malus spectabilis water extract are to CCL
4inducing hepatocyte damage synergistic protective effect (
n=6)
Note: * * represents that comparing P < 0.01, * represents and compare P < 0.05 with model group with model group
Result shows that Malus spectabilis and glossy ganoderma synergy are to CCL
4the hepatocellular injury of induction has good synergy.
Glossy ganoderma and Malus spectabilis water extract are to CCL
4cause the synergistic protective effect of acute hepatic injury mice:
Animal grouping and modeling method: get male mouse of kunming, random point in groups, and often organize 10, gastric infusion, Normal group and model group give the water of respective volume, continuous gastric infusion 4 days.1h after last administration, except Normal group lumbar injection (ip) normal saline, all the other respectively organize equal ip0.1%CCl
4peanut oil solution 0.1ml/10g.After fasting 16h, administration weighing again, after half an hour, all mouse pluck eyeball blood sampling, and separation of serum measures glutamic-pyruvic transaminase (ALT).The concentration of glossy ganoderma or ganoderma spove powder water extract is 80mg/kg, and the concentration of crab apple extract is 50mg/kg, 100mg/kg and 200mg/kg.
Table 2 glossy ganoderma and Malus spectabilis water extract are to CCL
4cause acute hepatic injury mice synergistic protective effect (
n=6)
Note: * * represents that comparing P < 0.01, * represents and compare P < 0.05 with model group with model group
Result shows that Malus spectabilis and glossy ganoderma synergy are to CCL
4the inhibitory action of the acute hepatic injury mice serum alt of induction is applied separately apparently higher than both, shows both and has good synergy.
Embodiment 2
Get ganoderma lucidium spore powder wall breaking, separately get Malus spectabilis tealeaves respectively at the baking oven inner drying 20-40min being preheated to 60-80 DEG C, control moisture below 8%, pulverize dried lucidum spore powder and dried Malus spectabilis tealeaves respectively, and cross 20-60 order; By weight, after 10 parts ~ 200 parts lucidum spore powders are mixed with 80 parts ~ 120 Malus spectabilis, again pulverize and sieve, and cross 80-120 order, pack often to wrap 0.2-5g.
Glossy ganoderma and crab apple extract are to the synergistic protective effect of chronic alcohol liver injury mouse:
Get male mouse of kunming, random point in groups, and often organize 10, gastric infusion, Normal group and model group give the water of respective volume.Except Normal group, every morning gavage 38 ° of white wine (0.1ml/10g), continuous 40d.After fasting last day 16h, administration weighing again, after half an hour, all eyeball of mouse blood samplings, separation of serum, measures serum glutamic pyruvic transminase (ALT).The concentration of glossy ganoderma or ganoderma spove powder water extract is 80mg/kg, and the concentration of crab apple extract is 50mg/kg, 100mg/kg and 200mg/kg.
Table 3 glossy ganoderma and Malus spectabilis water extract to the synergistic protective effect of chronic alcohol liver injury mouse (
n=6)
Note: * * represents that comparing P < 0.01, * represents and compare P < 0.05 with model group with model group
Result shows that Malus spectabilis and glossy ganoderma synergy are applied separately apparently higher than both the inhibitory action of ALT in chronic alcohol liver injury mice serum, shows both and has good synergy.
Embodiment 3
Make potato culture medium: potato 200g, glucose 20g, agar 20g, water 1000ml, be cut into small pieces by potato and put into pot, add water 1000ml, boils 20 minutes, by filtered through gauze, moisturizing, to 1000ml, puts into agar and glucose, heats after dissolving, pH to 6.0 is adjusted with potassium dihydrogen phosphate or dipotassium hydrogen phosphate solution, by in culture medium packing conical flask, often bottled enter 1/3 capacity culture medium, 0.1-0.15MPa steam high-voltage sterilizing 30min;
Actication of culture: take out lucidum strain from strain library, inoculates on aseptic operating platform, uses shaking table shaken cultivation, rotating speed 150r/min, temperature 25 DEG C, cultivates 5-7 days;
Make seed culture fluid: glucose 20g, gypsum 2g, analysis for soybean powder 10g, adjust pH to 6.0 with potassium dihydrogen phosphate or dipotassium hydrogen phosphate solution after malt flour 2g, 20% potato juice 1000ml prepare, be sub-packed in the triangular flask of 500ml, every bottled 150ml, autoclaving, inoculates the bacterial classification 5-10ml of activation on aseptic operating platform, use shaking table shaken cultivation, rotating speed 120r/min, temperature 25 DEG C, cultivates 5-7 days;
Inoculation and cultivation: Hubei Chinese flowering crabapple tealeaves is loaded in culture vessel, adds the water infiltration of 0.6-1.5 times of weight, sterilizing 20-30min at 121 DEG C, after taking out culture vessel, aseptically inoculate seed liquor 5-10ml, 20-30 DEG C and cultivate 10-30 days, at 121 DEG C, sterilizing 30min;
The preparation of tea in bag: the Hubei Chinese flowering crabapple fermented tea after sterilizing, at the baking oven inner drying 20-40min being preheated to 60-80 DEG C, controls moisture below 8%, pulverizes dried glossy ganoderma and dried Hubei Chinese flowering crabapple fermented tea respectively, and cross 20-60 order; By weight, after 10 parts ~ 200 parts lucidum spore powders are mixed with 80 parts ~ 120 Hubei Chinese flowering crabapples, again pulverize and sieve, and cross 80-120 order, pack often to wrap 0.2-5g.
The collaborative estrogen action of crab apple extract and Ganodenna Lucidum P.E:
The MCF-7 cell (human breast cancer cell, to estrogen sensitive) of taking the logarithm growth period, adjustment cell concentration to 1 × 10
4individual/ml, is inoculated in 96 orifice plates, cultivates make it adherent in 24 hours with the DMEM nutrient solution containing 10% calf serum.Culture medium is changed into without phenol red culture medium, then add variable concentrations medicine, the medicinal E2 of positive control (17 β – estradiol).37 DEG C, 5%CO in incubator
2cultivate 4 days, measure cell proliferation: sucking-off culture medium with mtt assay, every hole adds 0.2%MTT30 μ l, continues to cultivate 4h, sucking-off MTT, and every hole adds DMSO100 μ l, measures OD in 540nm.Result is as follows, and all data all represent by mean+SD, carries out statistical procedures with variance analysis.
The MDA-MB-231 cell (human breast cancer cell lacks ERs) of taking the logarithm growth period, adjustment cell concentration to 1 × 10
4individual/ml, is inoculated in 96 orifice plates, cultivates make it adherent in 24 hours with the L-15 nutrient solution containing 10% calf serum.Culture medium is changed into without phenol red culture medium, then add variable concentrations medicine, the medicinal E2 of positive control (17 β – estradiol).37 DEG C, 5%CO in incubator
2cultivate 4 days, measure cell proliferation: sucking-off culture medium with mtt assay, every hole adds 0.2%MTT30 μ l, continues to cultivate 4h, sucking-off MTT, and every hole adds DMSO100 μ l, measures OD in 540nm.The results are shown in Table 4, all data all represent by mean+SD, carry out statistical procedures with variance analysis.
The impact of table 4 Malus spectabilis water extract and Ganodenna Lucidum P.E on cell proliferation (
n=6)
Table 5 glossy ganoderma and Malus spectabilis water extract to the collaborative proliferation function of MCF-7 cell (
n=6)
Result shows that Malus spectabilis and glossy ganoderma synergy have estrogen action, has the same promotion cel l proliferation of same estrogen to estrogen sensitive cell line.Do not have Promote cell's growth effect to the breast cancer cell lacking ERs, synergy is applied separately apparently higher than both, shows both and has good synergy.
Glossy ganoderma and crab apple extract are on the impact of hyperlipemia in mice blood fat:
Get 50 male KM mouse, body weight 18-22g, be divided into 5 groups immediately according to digits table, often organize 10.Be respectively blank group, give general basal feed and feed; Hyperlipidemia model group: give High-fat diet (basal feed adds 10% lard, 10% yolk powder and 1% cholesterol).Effects of Xuezhikang group: gastric infusion every day (1.0g/kg) while giving high fat diet; Glossy ganoderma water extract group: gastric infusion every day (80mg/kg) while giving high fat diet, ganoderma lucidum fermented liquid group: gastric infusion every day (80mg/kg) while giving high fat diet, lucidum spore powder group: gastric infusion every day (80mg/kg) while giving high fat diet, Malus spectabilis water extract low (50mg/kg), in (100mg/kg), high dose group (200mg/kg), feed 3 weeks altogether, blank group and hyperlipidemia model group give isopyknic physiological saline.
Weigh after last administration, water 12h is can't help in fasting, and arteria carotis gets blood, centrifuging and taking serum, and automatic biochemical analyzer measures the content of serum total cholesterol (TC), triglycerides (TG).
Result shows: Malus spectabilis water extract obviously can reduce serum total cholesterol (TC), triglycerides (TG) (P<0.05 or P<0.01).
Table 6 Malus spectabilis water extract on the impact of hyperlipemia in mice blood fat (
n=8)
Note: compare * p<0.05 with model group, * * P<0.01
Table 7 glossy ganoderma and Malus spectabilis water extract to the synergy of serum total cholesterol (
n=8)
Table 8 glossy ganoderma and Malus spectabilis water extract to the synergy of triglycerides (
n=8)
Result has the content reducing hyperlipemia in mice serum total cholesterol (TC), triglycerides (TG) after showing Malus spectabilis and glossy ganoderma synergy, synergy is applied separately apparently higher than both, shows both and has good synergy.
Glossy ganoderma and crab apple extract are on the impact of blood glucose in diabetic mice:
Male mouse of kunming adaptability is fed 1 week, then high glucose and high fat feed (feed forms: 10% lard, 20% sucrose, 5% yolk, 65% conventional feed) is adopted to feed 8 weeks, water 12h is can't help in fasting, with 100mg/kg lumbar injection 2% alloxan, alloxan adopts the water-soluble solution of single steaming, now with the current.Mouse continues to feed high glucose and high fat feed, and after 72h (fasting 12h), mouse orbit rear vein beard is got blood and measured blood glucose value, chooses blood glucose value and is greater than the mouse of 11.1mmol/l as diabetic mice.Diabetic mice is divided into 5 groups at random by blood sugar and body weight, that is: Normal group, model control group, positive drug group (melbine 200mg/kg) and crab apple extract low (50mg/kg), in (100mg/kg), high dose group (200mg/kg), often organize 8.Continuous gastric infusion 4 weeks, blank group and hyperlipidemia model group give isopyknic physiological saline, and except Normal group gives conventional feed nursing, all the other are respectively organized all with high glucose and high fat forage feed.
Administration is after 4 weeks, and water 12h is can't help in blood sampling 21:00 fasting the previous day, the blood sampling of second day early 9:00 excision eyeball, and blood 3000r/min refrigerated centrifuge 10min, careful serum of drawing is put in other EP pipe, saves backup in-20 DEG C.After blood sampling, mouse cervical dislocation is put to death, and dissects and takes out pancreas, kidney, liver, musculature, puts-20 DEG C of low temperature refrigerators and preserves., filter paper clean with ice physiological saline is wiped dry, and make 10% tissue homogenate after weighing, in 4 DEG C, 3000r/min refrigerated centrifuge 10min, gets supernatant for subsequent use.Adopt Glucose estimation kit blood-sugar content.
Table 9 crab apple extract is on the impact of blood glucose in diabetic mice
Table 8 glossy ganoderma and Malus spectabilis water extract to blood glucose in diabetic mice synergy (
n=8)
Result have after showing Malus spectabilis and glossy ganoderma synergy reduce blood glucose in diabetic mice content, synergy is applied separately apparently higher than both, shows both to have good synergy.
Get Hubei Chinese flowering crabapple leaf 3-10 part, or the Hubei Chinese flowering crabapple fermented tea 3-10 part after sterilizing, or get Hubei Chinese flowering crabapple leaf 3-10 part and add lucidum spore powder 0.5-3 part, add 10-30 water 50-100 DEG C of heating doubly and extract 2-3 time or 10-30 50-95% (v/v) alcohol reflux extraction doubly 2-3 time.Filtrate processes through one of following method.
1, normal pressure or reduced pressure concentration obtain dry extract, and to cross 60-100 mesh sieve for subsequent use through being ground into powder for medicinal extract.
2, normal pressure or reduced pressure concentration obtain thick medicinal extract, and thick medicinal extract freeze drying obtains powder, cross 60-100 mesh sieve for subsequent use.
3, normal pressure or be evaporated to solid content 20-40%, spraying dry obtains powder, crosses 60-100 mesh sieve for subsequent use.Spray drying condition: solid content 15-30%; Baking temperature is that EAT selects about 160-200 DEG C, about outlet temperature 70-110 DEG C.Flow regulates according to equipment performance, ensures out temperature.
Get one of above-mentioned 3 kinds of methods gained dried powder 1000-10000 part, add starch and 1% dolomol, compressing tablet, makes every sheet containing phloridzin 5-40mg (auxiliary material used can be one or more in the auxiliary material commonly used of the tablets such as microcrystalline cellulose, modified starch, ethyl cellulose, methylcellulose, hydroxyethylcellulose, hydroxypropyl methylcellulose, sodium carboxymethylcellulose).Through tablet press machine tabletted, the heavy 0.5-2g of sheet.
Get one of above-mentioned 3 kinds of methods gained dried powder 1000-10000 part, add auxiliary material, granulate, whole grain, encapsulated, containing phloridzin 5-40mg (auxiliary material used can be one or more in the auxiliary material commonly used of the capsules such as microcrystalline cellulose, modified starch, ethyl cellulose, methylcellulose, hydroxyethylcellulose, hydroxypropyl methylcellulose, sodium carboxymethylcellulose) in every capsules.
Get one of above-mentioned 3 kinds of methods gained dried powder 1000-10000 part, add auxiliary material, granulate, whole grain, pack.Containing phloridzin 5-40mg (auxiliary material used can in the auxiliary material commonly used of the granule such as α galactolipin, beta cyclodextrin one or more) in every bag.
The preparation of phloridzin:
Get one of above-mentioned 3 kinds of methods gained dried powder 1000-10000 part, add auxiliary material, pack.Containing phloridzin 5-40mg (auxiliary material used can be one or more in the edible conventional auxiliary material such as microcrystalline cellulose, modified starch, ethyl cellulose, methylcellulose, hydroxyethylcellulose, hydroxypropyl methylcellulose, sodium carboxymethylcellulose, lactose) in every bag.
Claims (10)
1. a glossy ganoderma Chinese flowering crabapple tea, is characterized in that: this glossy ganoderma Chinese flowering crabapple tea is glossy ganoderma powder Malus spectabilis tea in bag or lucidum spore powder Malus spectabilis tea in bag or glossy ganoderma fermentation Malus spectabilis tea in bag.
2. the preparation technology of glossy ganoderma Chinese flowering crabapple tea according to claim 1, is characterized in that: described glossy ganoderma Chinese flowering crabapple tea is instant tea pulvis or granule or tablet or capsule or solution.
3. the preparation technology of glossy ganoderma Chinese flowering crabapple tea according to claim 1, it is characterized in that: the preparation technology of glossy ganoderma powder Malus spectabilis tea in bag is: get ganoderma lucidum fruitbody section, separately get Malus spectabilis tealeaves respectively at the baking oven inner drying 20min-40 min being preheated to 60-80 DEG C, control moisture below 8%, pulverize the section of dried ganoderma lucidum fruitbody and dried Malus spectabilis tealeaves respectively, and cross 20 order-60 orders; By weight, after 10 parts of-200 parts of glossy ganodermas are mixed with 80 parts of-120 parts of Malus spectabilis, again pulverize and sieve, and cross 80 order-120 orders, pack often to wrap 0.2g-5g.
4. the preparation technology of glossy ganoderma Chinese flowering crabapple tea according to claim 1, it is characterized in that: the preparation technology of lucidum spore powder Malus spectabilis tea in bag is: get ganoderma lucidium spore powder wall breaking, separately get Malus spectabilis tealeaves and be preheated to the baking oven inner drying 20-40 min of 60-80 DEG C respectively, control moisture below 8%, pulverize dried lucidum spore powder and dried Malus spectabilis tealeaves respectively, and cross 20-60 order; By weight, after 10 parts of-200 parts of lucidum spore powders are mixed with 80 parts of-120 parts of Malus spectabilis, again pulverize and sieve, and cross 80 order-120 orders, pack often to wrap 0.2-5g.
5. the preparation technology of glossy ganoderma Chinese flowering crabapple tea according to claim 1, is characterized in that, the preparation technology of glossy ganoderma fermentation Malus spectabilis tea in bag is:
1) potato culture medium is made: potato 200g, glucose 20g, agar 20g, water 1000ml, be cut into small pieces potato and put into pot, and add water 1000ml, boil 20 minutes, by filtered through gauze, moisturizing, to 1000ml, puts into agar and glucose, heat after dissolving, adjust pH to 6.0 with potassium dihydrogen phosphate or dipotassium hydrogen phosphate solution, by culture medium packing conical flask, often bottled enter 1/3 capacity culture medium, 0.1MPa-0.15MPa steam high-voltage sterilizing 30min, obtained potato culture medium;
2) actication of culture: take out lucidum strain from strain library, inoculates on aseptic operating platform, uses shaking table shaken cultivation, rotating speed 150 r/min, temperature 25 DEG C, cultivates 5-7 days, the bacterial classification of obtained activation;
3) seed culture fluid is made: glucose 20g, gypsum 2g, analysis for soybean powder 10g, adjust pH to 6.0 with potassium dihydrogen phosphate or dipotassium hydrogen phosphate solution after malt flour 2 g, 20% potato juice 1000ml prepare, be sub-packed in the triangular flask of 500ml, every bottled 150ml, autoclaving, inoculates the bacterial classification 5 ml-10ml of activation on aseptic operating platform, use shaking table shaken cultivation, rotating speed 120 r/min, temperature 25 DEG C, cultivates 5-7 days;
4) inoculation and cultivation: Malus spectabilis tealeaves is loaded in culture vessel, add the water infiltration of 0.6-1.5 times of Malus spectabilis tealeaves weight, sterilizing 20min-30 min at 121 DEG C, after taking out culture vessel, aseptically inoculate seed liquor 5 ml-10ml, cultivate 10-30 days, at 121 DEG C for 20-30 DEG C, sterilizing 30min, the Malus spectabilis fermented tea after obtained sterilizing;
5) preparation of tea in bag: the Malus spectabilis fermented tea after sterilizing is at baking oven inner drying 20 min-40 min being preheated to 60-80 DEG C, control moisture below 8%, pulverize dried glossy ganoderma powder and dried Malus spectabilis fermented tea respectively, and cross 20 order-60 orders, by weight, after 10 parts of-200 parts of glossy ganoderma powder are mixed with 80 parts-120 parts Malus spectabilis fermented teas, again pulverize and sieve, and cross 80 order-120 orders, pack often to wrap 0.2g-5g.
6. the glossy ganoderma Chinese flowering crabapple tea according to claim 1-5 any one claim, is characterized in that: described Malus spectabilis is Hubei Chinese flowering crabapple.
7. the application of the glossy ganoderma Chinese flowering crabapple tea according to claim 1-5 any one claim in preparation treatment or prevention in vitro liver cell damage.
8. the application of the glossy ganoderma Chinese flowering crabapple tea according to claim 1-5 any one claim in preparation treatment or preventing chronic alcoholic liver cellular damage.
9. the application of the glossy ganoderma Chinese flowering crabapple tea according to claim 1-5 any one claim in preparation treatment or prevention breast cancer.
10. the glossy ganoderma Chinese flowering crabapple tea according to claim 1-5 any one claim is reducing the application on blood fat, blood sugar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410279484.8A CN104839382B (en) | 2014-06-20 | 2014-06-20 | A kind of ganoderma lucidum Chinese flowering crabapple tea and preparation technology and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410279484.8A CN104839382B (en) | 2014-06-20 | 2014-06-20 | A kind of ganoderma lucidum Chinese flowering crabapple tea and preparation technology and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104839382A true CN104839382A (en) | 2015-08-19 |
| CN104839382B CN104839382B (en) | 2018-01-16 |
Family
ID=53839682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410279484.8A Active CN104839382B (en) | 2014-06-20 | 2014-06-20 | A kind of ganoderma lucidum Chinese flowering crabapple tea and preparation technology and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104839382B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105998093A (en) * | 2016-07-12 | 2016-10-12 | 周庆新 | Blood-glucose-lowering ganoderma lucidum instant micro-encapsulated preparation and preparation method thereof |
| CN108308333A (en) * | 2018-03-22 | 2018-07-24 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of golden flower Malus spectabilis tea production technology |
| CN110089597A (en) * | 2018-01-30 | 2019-08-06 | 恩施硒海棠生物科技有限公司 | A kind of preparation method of mulberry leaf Chinese flowering crabapple tea |
| CN112772748A (en) * | 2021-03-10 | 2021-05-11 | 江西仙客来生物科技有限公司 | Preparation method of ganoderma Lushan mist tea |
Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1105523A (en) * | 1994-07-07 | 1995-07-26 | 冯选凹 | Glossy ganoderma tea and its preparation method |
| CN1181901A (en) * | 1997-11-03 | 1998-05-20 | 黄少云 | Preparation process and product of nutritive glossy ganoderma liquid |
| CN1227062A (en) * | 1998-02-27 | 1999-09-01 | 福建省农业科学院土壤肥料研究所 | Technology for producing fermented glossy ganoderma tea |
| CN1351839A (en) * | 2000-11-09 | 2002-06-05 | 李绪荣 | Hubei Haitang red tea drink and producing method |
| CN1376397A (en) * | 2002-04-24 | 2002-10-30 | 王俊清 | Ganoderma tea and its preparing process |
| CN101011096A (en) * | 2007-01-26 | 2007-08-08 | 彭定有 | Hubei Malus spectabilis tea |
| CN101095444A (en) * | 2007-07-13 | 2008-01-02 | 福建仙芝楼生物科技有限公司 | Ganoderma lucidum bag tea and the processing technics thereof |
| CN101199301A (en) * | 2007-10-26 | 2008-06-18 | 三峡大学 | Hubei Chinese flowering crabapple tea and preparation method and application of extractive thereof |
| CN101233887A (en) * | 2008-02-28 | 2008-08-06 | 望宏端 | Method for preparing series health care cold tea from leaf of Hupeh crabapple |
| CN101411372A (en) * | 2007-10-17 | 2009-04-22 | 炉霍县林业开发总公司 | Health-care tea and its preparing process |
| CN101683411A (en) * | 2008-09-24 | 2010-03-31 | 汪鋆植 | Application of crab apple extract in preparing hepatic or health-care food |
| CN101897776A (en) * | 2009-06-01 | 2010-12-01 | 汪鋆植 | New application of Chinese flowing crabapple extractive and phlorizin |
| CN102228107A (en) * | 2011-05-13 | 2011-11-02 | 宜兴市鸿润食用菌种植专业合作社 | Ganoderma lucidum tea and preparation method thereof |
| CN102356791A (en) * | 2011-09-08 | 2012-02-22 | 张越梅 | Preparation method for ganoderma lucidum tea |
| KR101326162B1 (en) * | 2009-10-01 | 2013-11-07 | 재단법인 제주테크노파크 | Anti-inflammatory Composition |
| CN103719423A (en) * | 2014-01-26 | 2014-04-16 | 山东中信生物科技有限公司 | Lucid ganoderma tea and preparation method thereof |
-
2014
- 2014-06-20 CN CN201410279484.8A patent/CN104839382B/en active Active
Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1105523A (en) * | 1994-07-07 | 1995-07-26 | 冯选凹 | Glossy ganoderma tea and its preparation method |
| CN1181901A (en) * | 1997-11-03 | 1998-05-20 | 黄少云 | Preparation process and product of nutritive glossy ganoderma liquid |
| CN1227062A (en) * | 1998-02-27 | 1999-09-01 | 福建省农业科学院土壤肥料研究所 | Technology for producing fermented glossy ganoderma tea |
| CN1351839A (en) * | 2000-11-09 | 2002-06-05 | 李绪荣 | Hubei Haitang red tea drink and producing method |
| CN1376397A (en) * | 2002-04-24 | 2002-10-30 | 王俊清 | Ganoderma tea and its preparing process |
| CN101011096A (en) * | 2007-01-26 | 2007-08-08 | 彭定有 | Hubei Malus spectabilis tea |
| CN101095444A (en) * | 2007-07-13 | 2008-01-02 | 福建仙芝楼生物科技有限公司 | Ganoderma lucidum bag tea and the processing technics thereof |
| CN101411372A (en) * | 2007-10-17 | 2009-04-22 | 炉霍县林业开发总公司 | Health-care tea and its preparing process |
| CN101199301A (en) * | 2007-10-26 | 2008-06-18 | 三峡大学 | Hubei Chinese flowering crabapple tea and preparation method and application of extractive thereof |
| CN101233887A (en) * | 2008-02-28 | 2008-08-06 | 望宏端 | Method for preparing series health care cold tea from leaf of Hupeh crabapple |
| CN101683411A (en) * | 2008-09-24 | 2010-03-31 | 汪鋆植 | Application of crab apple extract in preparing hepatic or health-care food |
| CN101897776A (en) * | 2009-06-01 | 2010-12-01 | 汪鋆植 | New application of Chinese flowing crabapple extractive and phlorizin |
| KR101326162B1 (en) * | 2009-10-01 | 2013-11-07 | 재단법인 제주테크노파크 | Anti-inflammatory Composition |
| CN102228107A (en) * | 2011-05-13 | 2011-11-02 | 宜兴市鸿润食用菌种植专业合作社 | Ganoderma lucidum tea and preparation method thereof |
| CN102356791A (en) * | 2011-09-08 | 2012-02-22 | 张越梅 | Preparation method for ganoderma lucidum tea |
| CN103719423A (en) * | 2014-01-26 | 2014-04-16 | 山东中信生物科技有限公司 | Lucid ganoderma tea and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 无: "别派灵芝海棠茶", 《别派灵芝海棠茶_保健品_菌菇产品_中国第一菌物博物馆，HTTP://WWW.ZGJWBWG.COM/CHANPIN/SHOUW-17.HTML》 * |
| 无: "别派破壁灵芝孢子粉", 《别派破壁灵芝孢子粉_保健品_菌产品_供应_中国第一菌物博物馆，HTTP://WWW.ZGJWBWG.COM/CHANPIN/SHOUW-22.HTML》 * |
| 林旭东等: "灵芝保健袋泡茶的研制", 《农产品加工•学刊》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105998093A (en) * | 2016-07-12 | 2016-10-12 | 周庆新 | Blood-glucose-lowering ganoderma lucidum instant micro-encapsulated preparation and preparation method thereof |
| CN105998093B (en) * | 2016-07-12 | 2019-06-25 | 日照职业技术学院 | A kind of instant microencapsulated formulation of lucid ganoderma for reducing blood sugar and preparation method thereof |
| CN110089597A (en) * | 2018-01-30 | 2019-08-06 | 恩施硒海棠生物科技有限公司 | A kind of preparation method of mulberry leaf Chinese flowering crabapple tea |
| CN108308333A (en) * | 2018-03-22 | 2018-07-24 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of golden flower Malus spectabilis tea production technology |
| CN112772748A (en) * | 2021-03-10 | 2021-05-11 | 江西仙客来生物科技有限公司 | Preparation method of ganoderma Lushan mist tea |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104839382B (en) | 2018-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vattem et al. | Ellagic acid production and phenolic antioxidant activity in cranberry pomace (Vaccinium macrocarpon) mediated by Lentinus edodes using a solid-state system | |
| CN101683411B (en) | Application of crab apple extract in preparing hepatic or health-care food | |
| CN105996022A (en) | Method for producing functional foods by simultaneously converting Chinese yam from cordyceps taishanensis | |
| CN105105110A (en) | Making method of monascus and tartary buckwheat functional food and application thereof | |
| CN104839382B (en) | A kind of ganoderma lucidum Chinese flowering crabapple tea and preparation technology and its application | |
| CN106174221A (en) | A kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food | |
| KR101851195B1 (en) | Fermented mulberry leaves, femented mulberry leaves extract and use there of | |
| CN106136136A (en) | A kind of method utilizing Monascus anka Nakazawa et sato to convert Rhizoma Dioscoreae production functional food | |
| CN102613330A (en) | Fermented red yeast tea and extract, extract composition and preparation method thereof | |
| CN102613331B (en) | Halimasch fermented tea and halimasch fermented tea composition, and preparation methods thereof | |
| CN106615431A (en) | Sliced helianthus tuberosus tea and making method thereof | |
| KR101326271B1 (en) | The manufacturing method of fermented citrus peel extract with anti-obesity nature and the product | |
| WA et al. | Bioactive potential of some fascinating edible mushrooms Macrolepiota, Russula, Amanita, Vovariella and Grifola as a treasure of multipurpose therapeutic natural product | |
| KR20180093488A (en) | Method for manufacturing JuksongwonCompuond-P through frement | |
| CN103961534A (en) | Method for preparing cordyceps polysaccharide biological preparation | |
| CN102206692A (en) | Process for increasing extraction amount of baicilin by using traditional Chinese medicine fermentation technology | |
| CN113115942A (en) | Blood fat reducing dietary formula and processing technology thereof | |
| KR101745597B1 (en) | Fermented persimmon leaves, femented persimmon leaves extract and use there of | |
| CN106421208B (en) | Pharmaceutical composition with chemical liver injury resistance function and preparation method thereof | |
| KR101935689B1 (en) | Composition comprising mixture extract of jujube, licorice and platycodon for enhancing immune response | |
| CN102940650A (en) | Propolis ethanol extract for dispeling the effects of alcohol, preparation method of propolis ethanol extract and application of propolis ethanol extract for producing enteric-coated tablets | |
| Arora | Cordyceps sinensis (berk.) sacc.-an entomophagous medicinal fungus-a review | |
| CN102613367B (en) | Tricholoma matsutake fermented tea and preparation method thereof | |
| CN109549034A (en) | A kind of ganoderma lucidum composite health care beverage and preparation method thereof | |
| Liu et al. | Cultivation, Bioactive Metabolites, and Application of Caterpillar Mushroom Cordyceps militaris: Current State, Issues, and Perspectives |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20171221 Address after: 443002 Yichang University Road, Hubei, No. 8 Applicant after: China Three Gorges University Address before: 443002 Yichang University Road, Hubei, No. 8 Applicant before: China Three Gorges University Applicant before: Shennongjia Tian Run biotechnology Co., Ltd |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180403 Address after: Bachu Cultural Plaza 6 No. 444300 Hubei province Bashan Enshi Tujia and Miao Autonomous Prefecture Badong County Xin Ling Zhen Xi Rangpo Community Road 1 Building 2 floor Patentee after: Enshi selenium Begonia Biological Technology Co., Ltd. Address before: 443002 Yichang University Road, Hubei, No. 8 Patentee before: China Three Gorges University |