CN104825625B - Antiinflammatory for animals and preparation method thereof - Google Patents
Antiinflammatory for animals and preparation method thereof Download PDFInfo
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- CN104825625B CN104825625B CN201510116698.8A CN201510116698A CN104825625B CN 104825625 B CN104825625 B CN 104825625B CN 201510116698 A CN201510116698 A CN 201510116698A CN 104825625 B CN104825625 B CN 104825625B
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Abstract
Antiinflammatory for animals and preparation method thereof, it is that the active ingredient in pseudo-ginseng, felwort, radix stellariae dichotomae, fish raw meat, rheum officinale, kuh-seng, dandelion, the seed of cowherb, the Chinese medicine material such as play both sides of the street is drawn using bio-fermentation engineering and Biochemical Engineering technology, and by special process and a kind of multi-functional antiinflammatory for animals being configured to.The antiinflammatory can be safe efficient, quick while kills the major class pathogenic microorganisms of bacterium, fungi, virus, mycoplasma, Chlamydia, protozoon etc. six (including staphylococcus aureus, Escherichia coli, Candida albicans, vagina herpesviral, Ureaplasma urealyticum, vagina Chlamydia and Trichomonas vaginalis etc.).Antibacterial range, which substantially exceeds antibiotic, can only suppress to kill the category of bacterium, and no matter during production or use, equal contamination-free produces.And cost is low, effect is good, has no toxic side effect.Antibiotic can be substituted and eliminate harm to human body and domestic animals of " anti-milk " and " anti-meat ", and effectively treatment, prevention ox, horse, sheep, pig, dog, genital inflammation and mammitis of wild animal and pet etc. etc., market prospects are boundless.
Description
Technical field
The invention belongs to apply the modern traditional Chinese patent medicine manufacturing technology field of bio-fermentation engineering and Biochemical Engineering technology,
More particularly to using Chinese medicine as modern antiinflammatory for animals of raw material and preparation method thereof.
Background technology
Since " melamine event " occurs for China, country has repeated injunctions, and forbids ox of the sale containing " anti-milk "
Milk and its dairy products and " anti-meat " etc. (milk and meat containing antibiotic claim " anti-milk " and " anti-meat "), the use range of antibiotic also by
To strict limitation.But it in the current marketplace, can effectively treat, prevent the reproductions such as ox, horse, sheep, pig, dog, wild animal and pet
The medicine of road inflammation and mammitis only has antibiotic, and the effect that other medicines have is poor, and some toxic side effects are too big, can not also
Substitute antibiotic.Therefore antibiotic can be substituted there is an urgent need to one kind and eliminate the harm of " anti-milk " and " anti-meat " to people and domestic animals,
And can effectively treat, prevent ox, horse, sheep, pig, dog, wild animal and pet etc. genital inflammation and mammitis it is new
Antiinflammatory for animals.
The content of the invention
It is an object of the invention to provide a kind of new antiinflammatory for animals and preparation method.Pass through application high-tech biology hair
The preparation method of ferment engineering technology and Biochemical Engineering technology draws pseudo-ginseng, felwort, radix stellariae dichotomae, fish raw meat, rheum officinale, kuh-seng, Pu Gong
Active ingredient in English, the seed of cowherb, the Chinese medicine material such as play both sides of the street, and it is configured to antiinflammatory by defined weight proportion；In the hope of
Substitute antibiotic with the antiinflammatory and eliminate harm to human body and domestic animals of " anti-milk " and " anti-meat ", and effectively treatment, prevention ox,
The inflammation such as horse, sheep, pig, dog, wild animal and pet.
The concrete technology preparation process of the present invention is such：
Step 1, first cleaned main material pseudo-ginseng, felwort, radix stellariae dichotomae, cordate houttuynia are respectively put into boiling vessel and soaked
Steep, boil, afterwards by four kinds of gained decoctions are filtered, are inoculated with respectively after sterilizing, cultivate carry out one time fermentation, then go out respectively
Bacterium, filtering, four kinds of zymotic fluids are made, secondary hair is carried out after then four kinds of zymotic fluids are well mixed by 1: 4: 2: 2 part by weight
Ferment culture, so as to produce the core antimicrobial fluid that can kill six major class pathogenic microorganisms, then sterilization treatment is carried out to the antimicrobial fluid and obtained
Obtain main material extract solution --- " glossy ganoderma fermentation antiinflammatory ".
The fermented bacterium of glossy ganoderma fermentation antiinflammatory is：" red ganoderma Ganoderma lucidum sp ".The strain is Yunnan
It is bright wait upon to conjecture up to biotechnology Co., Ltd week teach the plum ganoderma lucidum collected the eighties and (recorded according to ancient book, in Japanese apricot
The Wild ganoderma of lower growth is referred to as plum ganoderma lucidum, and its effective component is best), by biological fermentation engineering, genetic engineering, breeding technique
With the processing of mutation breeding technologies, turn out and be rich in ganoderma lucidum organic germanium, ganodenic acid alkene, the superior strain of GL-B, bacterium
Kind is now stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center.The specific following institute of biological deposits item
Show：
Depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center
Address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica
Preservation date：On July 2nd, 2010
Deposit number：CGMCC No.3982
Classification And Nomenclature：Ganoderma lucidum Ganoderma lucidum
One time fermentation described in the step includes Spawn incubation inclined plane inoculating, 22 ± 1 DEG C of cultivation temperature, is set using instrument
It is standby：Superclean bench, constant incubator, refrigerator etc., 7~12 days time.One-level culture shaking table or fermentation tank, it will be trained on inclined-plane
The lawn supported is broken up lawn with aseptic water washing and whirlpool concussion instrument, is seeded in triangular flask, with shaking table culture or inoculation
In one grade fermemtation tank, 22 ± 1 DEG C of cultivation temperature, incubation time 7~12 days.Secondary fermentation described in the step is trained including two level
Support：By the cultured strain of one-level, it is pumped into second order fermentation tank and cultivates, 22 ± 1 DEG C of cultivation temperature, incubation time 10~15 days.
Step 2, by cleaned auxiliary material rheum officinale, kuh-seng, dandelion, the seed of cowherb, playing both sides of the street is respectively put into boiling vessel
In soak, boil, filtering afterwards, sterilization treatment obtain five kinds of auxiliary material extract solutions.
Step 3, the glossy ganoderma fermentation antiinflammatory that step 1 and step 2 are obtained, Radix Et Rhizoma Rhei extract, Radix Sophorae Flavescentis extractive liquid,
Dandelion, the seed of cowherb, the extract solution that plays both sides of the street are well mixed by 3: 2: 2: 1: 1: 2 weight proportion.
It is using Chinese medicine as raw material, using biofermentation work made of above-mentioned preparation method " novel veterinary antiinflammatory "
Antiinflammatory prepared by journey technology and Biochemical Engineering technology is core material, adds rheum officinale, kuh-seng, dandelion, the seed of cowherb, Lu Lu
A kind of logical more effect antiinflammatories for waiting auxiliary material to obtain, can kill includes bacterium, fungi, virus, mycoplasma, Chlamydia, original
The major class pathogenic microorganisms of worm etc. six (including staphylococcus aureus, Escherichia coli, Candida albicans, vagina herpesviral, solution urea
Mycoplasma, vagina Chlamydia and Trichomonas vaginalis etc.).Antibacterial range, which substantially exceeds antibiotic, can only suppress to kill the category of bacterium,
It has been the breakthrough and innovation in antiseptic field since nineteen twenty-eight penicillin is found.
Substitute the endometritis of antibiotic therapy ox, horse, sheep, pig etc. with the medicament, after colpitis and mastitis,
Antibiotic is free of in the product of its meat and milk, thus " anti-milk " and " anti-meat " will not be caused to pollute, human body and domestic animals are caused
Harm.Production cost of the present invention is low, and effect is good, has no toxic side effect, and no matter during production or use, it is pollution-free
Thing produces.Therefore, the present invention is the new biocide product that national society's livestock breeding industry is badly in need of, and market prospects are boundless, general
And the development after promoting to China's livestock breeding industry will bring obvious economic benefit and social benefit.
Brief description of the drawings
Fig. 1 is the process chart of the embodiment of the present invention
Embodiment
Embodiments of the invention are illustrated below in conjunction with the accompanying drawings：
Refer to the attached drawing 1, in embodiments of the present invention, first by cleaned main material pseudo-ginseng, felwort, radix stellariae dichotomae, fish
Raw meat grass, which is respectively put into boiling vessel, to be soaked, boils, afterwards by four kinds gained decoctions it is filtered, sterilizing after use " red ganoderma respectively
The inoculation of Ganoderma lucidum sp " strains, culture carry out one time fermentation, are then sterilized separately, filter, four kinds of fermentations are made
Liquid, then by four kinds of zymotic fluids in 1: 4: 2: 2 ratios it is well mixed after carry out secondary fermentation culture, so as to produce, to kill six big
The core bactericidal liquid of class pathogenic microorganisms, then sterilization treatment is carried out to the bactericidal liquid and obtains main material extract solution --- " glossy ganoderma fermentation
Antiinflammatory ".Described one time fermentation includes inclined plane inoculating, Spawn incubation, 22 ± 1 DEG C of cultivation temperature, uses instrument and equipment：It is ultra-clean
Workbench, constant incubator, refrigerator, water treatment facilities, extraction equipment, filter plant, sterile working equipment etc., 10 days time.
One-level culture shaking table and 100 liter fermentation tanks, will by cultured lawn aseptic water washing on inclined-plane and whirlpool concussion instrument
Lawn is broken up, and is seeded in triangular flask, is inoculated with or is seeded in 100 liter one grade fermemtation tanks, cultivation temperature 22 with shaking table culture
± 1 DEG C, incubation time 10 days.Described secondary fermentation includes two level culture：By the cultured strain of one-level, second order fermentation is pumped into
Cultivated in tank, 22 ± 1 DEG C of cultivation temperature, incubation time 12 days.
Again by cleaned auxiliary material rheum officinale, kuh-seng, dandelion, the seed of cowherb, play both sides of the street to be respectively put into boiling vessel and soak
Steep, boil, filtering afterwards, sterilization treatment obtain five kinds of auxiliary material extract solutions.
Finally by the glossy ganoderma fermentation antiinflammatory obtained, Radix Et Rhizoma Rhei extract, Radix Sophorae Flavescentis extractive liquid, radix stellariae dichotomae extract solution by 3: 2:
2: 1: 1: 2 weight proportion is well mixed, and end-product has just been obtained after sterilizing --- novel veterinary antiinflammatory.
Embodiments of the invention application bio-fermentation engineering and Biochemical Engineering technology draw pseudo-ginseng, felwort, rheum officinale,
Kuh-seng, radix stellariae dichotomae, cordate houttuynia, play both sides of the street, the active ingredient in the Chinese medicine material such as seed of cowherb, this more effects being configured to
Antiinflammatory for animals, energy is safe efficient, quick while kills the major class of bacterium, fungi, virus, mycoplasma, Chlamydia, protozoon etc. six
Pathogenic microorganisms (including staphylococcus aureus, Escherichia coli, Candida albicans, vagina herpesviral, Ureaplasma urealyticum, the moon
Road Chlamydia and Trichomonas vaginalis etc.).Antibacterial range substantially exceed antibiotic can only suppress kill bacterium category, and no matter
During production or use, equal contamination-free produces.In a large amount of of many large-scale livestock-raising enterprises such as " Mongolia Ox ", " Erie "
In clinical practice case, it was demonstrated that the medicament is except can be safe efficient, quick while killing and causing ox, horse, sheep, pig, dog, wild
Outside six major class pathogenic microorganisms of the endometritis of animal and pet etc., colpitis and mastitis, also have and inflammation is disappeared
It is swollen, the medicine attribute and feature of anti-inflammatory.
Claims (2)
1. antiinflammatory preparation method for animals, it is characterised in that this method comprises the following steps：
Step 1, first cleaned main material pseudo-ginseng, felwort, radix stellariae dichotomae, cordate houttuynia are respectively put into boiling vessel and soaks, boil
Boiling, afterwards by four kinds of gained decoctions are filtered, are inoculated with, cultivate respectively after sterilizing, one time fermentation is carried out, is then sterilized separately, mistake
Filter, is made four kinds of zymotic fluids, and four kinds of zymotic fluids then are pressed into 1:4:2:Secondary fermentation training is carried out after 2 part by weight are well mixed
Support, so as to produce the core antimicrobial fluid that can kill six major class pathogenic microorganisms, then sterilization treatment is carried out to the antimicrobial fluid and led
Raw material extract solution --- " glossy ganoderma fermentation antiinflammatory "；
The fermented bacterium of the glossy ganoderma fermentation antiinflammatory is：" red ganoderma Ganoderma lucidum sp "；
Step 2, by cleaned auxiliary material rheum officinale, kuh-seng, dandelion, the seed of cowherb, play both sides of the street, be respectively put into boiling vessel
Soak, boil, filtering afterwards, sterilization treatment obtain five kinds of auxiliary material extract solutions；
Step 3, the main and auxiliary raw material extract solution that step 1 and step 2 are obtained are well mixed by weight ratio；
The mixed proportion by weight of four kinds of zymotic fluids of pseudo-ginseng, felwort, radix stellariae dichotomae, cordate houttuynia described in step 1 be 1 part of pseudo-ginseng,
4 parts of felwort, 2 parts of radix stellariae dichotomae, 2 parts of cordate houttuynia；
One time fermentation described in step 1 includes Spawn incubation, inclined plane inoculating, 22 ± 1 DEG C of cultivation temperature, 7~12 days time, and one
Level culture shaking table or fermentation tank；Described secondary fermentation：By the cultured strain of one-level, it is pumped into second order fermentation tank and cultivates,
22 ± 1 DEG C of cultivation temperature, incubation time 10~15 days.
A kind of 2. antiinflammatory for animals made of preparation method according to claim 1, it is characterised in that：The medicament is by following original
Material is mixed by weight ratio：3 parts of glossy ganoderma fermentation antiinflammatory, 2 parts of Radix Et Rhizoma Rhei extract, 2 parts of Radix Sophorae Flavescentis extractive liquid, dandelion extraction
1 part of liquid, 1 part of the seed of cowherb, play both sides of the street 2 parts.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510116698.8A CN104825625B (en) | 2015-03-18 | 2015-03-18 | Antiinflammatory for animals and preparation method thereof |
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| CN201510116698.8A CN104825625B (en) | 2015-03-18 | 2015-03-18 | Antiinflammatory for animals and preparation method thereof |
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| CN104825625A CN104825625A (en) | 2015-08-12 |
| CN104825625B true CN104825625B (en) | 2017-12-29 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106176955A (en) * | 2016-07-22 | 2016-12-07 | 太仓市爱农奶牛专业合作社 | A kind of antiinflammatory treating mammitis of cow and preparation method thereof |
| CN107384809A (en) * | 2017-09-05 | 2017-11-24 | 云南明侍达生物科技有限责任公司 | One plant of Ganoderma Lucidum and its application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1380098A (en) * | 2002-05-20 | 2002-11-20 | 张勇飞 | Anti-infection compound preparation and its preparation method |
| CN101439175A (en) * | 2008-12-25 | 2009-05-27 | 杭州万得富生物技术有限公司 | Chinese medicine preparation for treating nephropathogenic infectious bronchitis in chicken |
| CN102641474A (en) * | 2012-05-03 | 2012-08-22 | 青岛绿曼生物工程有限公司 | Compound propolis composition for treating chicken mycoplasmosis and preparation method thereof |
| CN104547234A (en) * | 2014-12-19 | 2015-04-29 | 张连 | Traditional Chinese medicine composition for preventing and treating fish liver-gall syndrome |
-
2015
- 2015-03-18 CN CN201510116698.8A patent/CN104825625B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1380098A (en) * | 2002-05-20 | 2002-11-20 | 张勇飞 | Anti-infection compound preparation and its preparation method |
| CN101439175A (en) * | 2008-12-25 | 2009-05-27 | 杭州万得富生物技术有限公司 | Chinese medicine preparation for treating nephropathogenic infectious bronchitis in chicken |
| CN102641474A (en) * | 2012-05-03 | 2012-08-22 | 青岛绿曼生物工程有限公司 | Compound propolis composition for treating chicken mycoplasmosis and preparation method thereof |
| CN104547234A (en) * | 2014-12-19 | 2015-04-29 | 张连 | Traditional Chinese medicine composition for preventing and treating fish liver-gall syndrome |
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