CN104825625A - Novel veterinary anti-inflammation agent and preparation method of same - Google Patents

Abstract

A novel veterinary anti-inflammation agent and a preparation method of the same. The multifunctional veterinary anti-inflammation agent is prepared by extracting effective components from following traditional Chinese medicine raw materials including: pseudo-ginseng, gentiana scabra, radix stellariae, herba houttuyniae, rhubarb, sophora flavescens, dandelion, cowherb seeds and liquidambar formosana and the like, by means of a bio-fermentation engineering technology and a bio-chemical engineering technology according to a special process and a special formula. The anti-inflammation agent can safely, high-effectively, quickly and synchronously kill six categories of pathogenic microorganisms including bacteria, fungus, viruses, mycoplasma, chlamydia and protozoons (including staphylococcus aureus, escherichia coli, candida albicans, herpes virus vaginalis, mycoplasma urealytium, chlamydia vaginalis, trichomonas vaginalis and the like). The anti-bacterial range of the anti-inflammation agent greatly exceeds a limit that conventional antibiotics only can kill and inhibit bacteria. The anti-inflammation agent is free of generation of polluting substances not only in production but also in use, is low in production cost, is good in effects and is free of toxic and side effects, can be used for replacing the antibiotics to eliminate damage on human body and livestock due to "antibiotic-milk" and "antibiotic-meat", can effectively prevent and treat inflammation ingenital tract and mammary gland of cattle, horses, sheep, pigs, dogs, wild animals and pets, and is very wide in market prospect.

Description

Novel veterinary antiinflammatory and preparation method thereof

Technical field

The invention belongs to the modern traditional Chinese patent medicine manufacturing technology field of applying biological fermentation engineering and biochemical engineering technology, is particularly modern times antiinflammatory for animals of raw material and preparation method thereof with Chinese crude drug.

Background technology

Since there is " tripolycyanamide event " in China, country repeats injunctions, forbid to sell containing the milk of " anti-milk " and milk product and " anti-meat " etc. (claiming " anti-milk " and " anti-meat " containing the milk of antibiotics and meat), the scope of application of antibiotics is also subject to strict restriction.But in the current marketplace, effectively can treat, prevent the medicine of the urogenital tract infections such as cattle, horse, sheep, pig, Canis familiaris L., wild animal and house pet and mammitis to only have antibiotics, the weak effect that other medicine has, some toxic and side effects are too large, also cannot substitute antibiotics.Therefore can substitute antibiotics in the urgent need to one and eliminate " anti-milk " and " anti-meat " harm to people and domestic animal, effectively can treat again, prevent the urogenital tract infection of cattle, horse, sheep, pig, Canis familiaris L., wild animal and house pet etc. and the Novel veterinary antiinflammatory of mammitis.

Summary of the invention

The object of this invention is to provide a kind of novel antiinflammatory for animals and preparation method.Drawn the effective ingredient in the raw material of Chinese medicine such as Radix Notoginseng, Radix Gentianae, Radix Stellariae, fish raw meat, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Herba Taraxaci, Semen Vaccariae, Fructus Liquidambaris by the preparation method applying high-tech bio-fermentation engineering and biochemical engineering technology, and be mixed with antiinflammatory by the weight proportion of regulation; Eliminate " anti-milk " and " anti-meat " harm to human body and domestic animal in the hope of substituting antibiotics with this antiinflammatory, and effectively treatment, prevent the inflammation such as cattle, horse, sheep, pig, Canis familiaris L., wild animal and house pet.

Concrete technology preparation process of the present invention is such:

Step one, first cleaned main material Radix Notoginseng, Radix Gentianae, Radix Stellariae, Herba Houttuyniae are put into immersion in boiling vessel respectively, boiled, afterwards by four kinds of gained medicinal liquids after filtration, inoculate respectively after sterilizing, cultivate and carry out one time fermentation, then sterilizing respectively, filtration, make four kinds of fermentation liquids, then four kinds of fermentation liquids are carried out ferment in second time cultivation by after 1: 4: 2: 2 part by weight mix homogeneously, thus produce the core antimicrobial fluid killing six large class pathogenic microorganism, then sterilization treatment acquisition main material extracting solution is carried out to this antimicrobial fluid---" glossy ganoderma fermentation antiinflammatory ".

The fermented bacterium of glossy ganoderma fermentation antiinflammatory is: " red ganoderma Ganoderma lucidum sp ".This strain is that the prunus mume (sieb.) sieb.et zucc. Ganoderma of teaching and collecting the eighties should be conjectured (according to Gu in week by Yunnan Ming Shida biotechnology Co., Ltd secretarycarry, the wild Ganoderma grown under Prunus mume (Sieb.) Sieb. Et Zucc is called prunus mume (sieb.) sieb.et zucc. Ganoderma, its active ingredient is best), through biological fermentation engineering, genetic engineering, the process of breeding technique and mutation breeding technologies, turn out and be rich in Ganoderma organic germanium, Ganoderma triterpenoids alkene, the superior strain of ganoderan, strain is now kept at Chinese microorganism strain preservationmanagement committeecommon micro-organisms center.Concrete biology preservationitem is as follows:

preservationunit: Chinese microorganism strain preservationmanagement committeecommon micro-organisms center

Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica

preservationdate: on July 2nd, 2010

preservationnumbering: CGMCC No.3982

Classification And Nomenclature: Ganoderma Ganoderma lucidum

One time fermentation described in this step comprises spawn culture inclined plane inoculating, cultivation temperature 22 ± 1 DEG C, uses instrument and equipment: superclean bench, constant incubator, refrigerator etc., 7 ~ 12 days time.One-level cultivation shaking table or fermentation tank, break up lawn aseptic water washing cultured on inclined-plane and whirlpool concussion instrument lawn, be seeded in triangular flask, cultivate or be seeded in one grade fermemtation tank, cultivation temperature 22 ± 1 DEG C, incubation time 7 ~ 12 days with shaking table.Ferment in second time described in this step comprises secondary and cultivates: by cultured for one-level strain, pump in second order fermentation tank and cultivate, cultivation temperature 22 ± 1 DEG C, incubation time 10 ~ 15 days.

Step 2, cleaned auxiliary material Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Herba Taraxaci, Semen Vaccariae, Fructus Liquidambaris are put into immersion in boiling vessel respectively, boiled, and filtration, sterilization treatment obtain five kinds of auxiliary material extracting solution afterwards.

Step 3, the glossy ganoderma fermentation antiinflammatory that step one and step 2 are obtained, Radix Et Rhizoma Rhei extract, Radix Sophorae Flavescentis extractive liquid, Herba Taraxaci, Semen Vaccariae, Fructus Liquidambaris extracting solution by 3: 2: 2: 1: 1: 2 weight proportion mix homogeneously.

" the Novel veterinary antiinflammatory " be made up of above-mentioned preparation method, take Chinese crude drug as raw material, antiinflammatory prepared by applying biological fermentation engineering and biochemical engineering technology is core material, add Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Herba Taraxaci, Semen Vaccariae, one many effects antiinflammatory that the auxiliary materials such as Fructus Liquidambaris obtain, can kill and comprise antibacterial, fungus, virus, mycoplasma, chlamydia, the large class pathogenic microorganism of protozoon etc. six (comprises staphylococcus aureus, escherichia coli, Candida albicans, vagina herpesvirus, Ureaplasma urealyticum, vagina chlamydia and trichomonas vaginitis etc.).Antibacterial range substantially exceeds the category that antibiotics can only suppress kill bacteria, has been since nineteen twenty-eight, penicillin found, the breakthrough in antibacterial field and innovation.

Substitute the endometritis of antibiotic therapy cattle, horse, sheep, pig etc. with this medicament, after colpitis and mastitis, all not containing antibiotics in the goods of its meat and milk, " anti-milk " and " anti-meat " thus can not be caused to pollute, human body and domestic animal are worked the mischief.Production cost of the present invention is low, effective, has no side effect, and no matter in production or use procedure, equal contamination-free generation.Therefore, the present invention is the new biocide product that national society's livestock breeding industry is badly in need of, and market prospect is boundless, will bring obvious economic benefit and social benefit after penetration and promotion to the development of China's livestock breeding industry.

Accompanying drawing explanation

Fig. 1 is the process chart of the embodiment of the present invention

Detailed description of the invention

Below in conjunction with accompanying drawing, embodiments of the invention are described:

With reference to accompanying drawing 1, in embodiments of the present invention, first by cleaned main material Radix Notoginseng, Radix Gentianae, Radix Stellariae, Herba Houttuyniae is put into respectively in boiling vessel and is soaked, boil, afterwards by four kinds of gained medicinal liquids after filtration, " red ganoderma Ganoderma lucidum sp " strain inoculation is used respectively after sterilizing, one time fermentation is carried out in cultivation, then sterilizing respectively, filter, make four kinds of fermentation liquids, then four kinds of fermentation liquids are carried out ferment in second time cultivation in after 1: 4: 2: 2 ratio mix homogeneously, thus produce the core bactericidal liquid killing six large class pathogenic microorganism, again sterilization treatment is carried out to this bactericidal liquid and obtains main material extracting solution---" glossy ganoderma fermentation antiinflammatory ".Described one time fermentation comprises inclined plane inoculating, spawn culture, cultivation temperature 22 ± 1 DEG C, uses instrument and equipment: superclean bench, constant incubator, refrigerator, water treatment facilities, extraction equipment, filter plant, sterile working's equipment etc., 10 days time.One-level cultivation shaking table and 100 liter fermentation tanks, lawn is broken up by lawn aseptic water washing cultured on inclined-plane and whirlpool concussion instrument, be seeded in triangular flask, cultivate inoculation with shaking table or be seeded in 100 liter one grade fermemtation tanks, cultivation temperature 22 ± 1 DEG C, incubation time 10 days.Described ferment in second time comprises secondary and cultivates: by cultured for one-level strain, pump in second order fermentation tank and cultivate, cultivation temperature 22 ± 1 DEG C, incubation time 12 days.

Again cleaned auxiliary material Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Herba Taraxaci, Semen Vaccariae, Fructus Liquidambaris are put into immersion in boiling vessel respectively, boiled, filtration, sterilization treatment obtain five kinds of auxiliary material extracting solution afterwards.

Finally by obtained glossy ganoderma fermentation antiinflammatory, Radix Et Rhizoma Rhei extract, Radix Sophorae Flavescentis extractive liquid, Radix Stellariae extracting solution by the weight proportion mix homogeneously of 3: 2: 2: 1: 1: 2, just obtain end-product after sterilizing---Novel veterinary antiinflammatory.

Embodiments of the invention applying biological fermentation engineering and biochemical engineering technology draw Radix Notoginseng, Radix Gentianae, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Radix Stellariae, Herba Houttuyniae, Fructus Liquidambaris, effective ingredient in the raw material of Chinese medicine such as Semen Vaccariae, this many effects antiinflammatory for animals be mixed with, can safety, efficient, fast, six large class pathogenic microorganism (comprising staphylococcus aureus, escherichia coli, Candida albicans, vagina herpesvirus, Ureaplasma urealyticum, vagina chlamydia and trichomonas vaginitis etc.) such as kill bacteria, fungus, virus, mycoplasma, chlamydia, protozoon simultaneously.Antibacterial range substantially exceeds the category that antibiotics can only suppress kill bacteria, and no matter in production or use procedure, equal contamination-free generation.In a large amount of clinical practice cases of many large-scale livestock-raising enterprises such as " Mongolia Ox ", " Erie ", prove that this medicament is except energy is safe, kill the endometritis causing cattle, horse, sheep, pig, Canis familiaris L., wild animal and house pet etc. efficiently, fast, simultaneously, outside six large class pathogenic microorganism of colpitis and mastitis, also have inflammation detumescence, the medicine attribute of antiinflammatory and feature.

Claims (5)

1. Novel veterinary antiinflammatory preparation method, is characterized in that, the method comprises the following steps:

Step one, first cleaned main material Radix Notoginseng, Radix Gentianae, Radix Stellariae, Herba Houttuyniae are put into immersion in boiling vessel respectively, boiled, afterwards by four kinds of gained medicinal liquids after filtration, inoculate respectively after sterilizing, cultivate, carry out one time fermentation, then sterilizing respectively, filtration, make four kinds of fermentation liquids, then ferment in second time cultivation is carried out by after four kinds of fermentation liquids by weight proportion mix homogeneously, thus produce the core antimicrobial fluid killing six large class pathogenic microorganism, then sterilization treatment acquisition main material extracting solution is carried out to this antimicrobial fluid---" glossy ganoderma fermentation antiinflammatory ";

Step 2, by cleaned auxiliary material Radix Et Rhizoma Rhei, Radix Sophorae Flavescentis, Herba Taraxaci, Semen Vaccariae, Fructus Liquidambaris, put into immersion in boiling vessel respectively, boil, filtration, sterilization treatment obtain five kinds of adjuvant extracting solution afterwards;

Step 3, the main and auxiliary raw material extracting solution mix homogeneously by weight ratio that step one and step 2 are obtained.

2. Novel veterinary antiinflammatory preparation method according to claim 1, is characterized in that: the mixed proportion by weight of the Radix Notoginseng described in step one, Radix Gentianae, Radix Stellariae, Herba Houttuyniae four kinds of fermentation liquids is Radix Notoginseng 1 part, Radix Gentianae 4 parts, Radix Stellariae 2 parts, Herba Houttuyniae 2 parts.

3. Novel veterinary antiinflammatory preparation method according to claim 1, it is characterized in that: the one time fermentation described in step one comprises spawn culture, inclined plane inoculating, cultivation temperature 22 ± 1 DEG C, 7 ~ 12 days time, lawn (is broken up by lawn aseptic water washing cultured on inclined-plane and whirlpool concussion instrument, is seeded in triangular flask by one-level cultivation shaking table or fermentation tank, cultivate with shaking table or be seeded in one grade fermemtation tank, cultivation temperature 22 ± 1 DEG C, incubation time 7 ~ 12 days); Described ferment in second time: by cultured for one-level strain, pump in second order fermentation tank and cultivate, cultivation temperature 22 ± 1 DEG C, incubation time 10 ~ 15 days.

4. the Novel veterinary antiinflammatory made of preparation method according to claim 1, is characterized in that: this medicament is mixed by weight ratio by following raw materials according: glossy ganoderma fermentation antiinflammatory 3 parts, Radix Et Rhizoma Rhei extract 2 parts, Radix Sophorae Flavescentis extractive liquid 2 parts, Herba Taraxaci extract 1 part, Semen Vaccariae 1 part, Fructus Liquidambaris 2 parts.

5. Novel veterinary antiinflammatory according to claim 4, is characterized in that: the fermented bacterium of glossy ganoderma fermentation antiinflammatory is: " red ganoderma Ganoderma lucidum sp ".