CN104825496A - Scapharca granosa extract and application thereof in medicine for promoting postpartum uterine contraction - Google Patents
Scapharca granosa extract and application thereof in medicine for promoting postpartum uterine contraction Download PDFInfo
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- CN104825496A CN104825496A CN201510183462.6A CN201510183462A CN104825496A CN 104825496 A CN104825496 A CN 104825496A CN 201510183462 A CN201510183462 A CN 201510183462A CN 104825496 A CN104825496 A CN 104825496A
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- extract
- blood clam
- numb
- uterine contraction
- clam extract
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- 208000036029 Uterine contractions during pregnancy Diseases 0.000 title claims abstract description 19
- 239000003814 drug Substances 0.000 title claims abstract description 13
- 230000001737 promoting effect Effects 0.000 title abstract description 4
- 241000237506 Scapharca Species 0.000 title 1
- 241001339782 Scapharca broughtonii Species 0.000 claims abstract description 31
- 229940079593 drug Drugs 0.000 claims abstract description 4
- 235000013372 meat Nutrition 0.000 claims abstract description 4
- 206010000210 abortion Diseases 0.000 claims abstract description 3
- 231100000176 abortion Toxicity 0.000 claims abstract description 3
- 238000002360 preparation method Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000002775 capsule Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 208000036365 Normal labour Diseases 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000006187 pill Substances 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 5
- 208000018525 Postpartum Hemorrhage Diseases 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 238000001356 surgical procedure Methods 0.000 abstract 1
- 210000004291 uterus Anatomy 0.000 description 11
- 239000000835 fiber Substances 0.000 description 6
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 6
- 229920002472 Starch Polymers 0.000 description 4
- 210000000754 myometrium Anatomy 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 235000019359 magnesium stearate Nutrition 0.000 description 3
- 241000628923 Anadara sativa Species 0.000 description 2
- 241000180579 Arca Species 0.000 description 2
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 description 2
- 101800000989 Oxytocin Proteins 0.000 description 2
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 description 2
- 208000002193 Pain Diseases 0.000 description 2
- 206010046820 Uterine rupture Diseases 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000008602 contraction Effects 0.000 description 2
- 230000001595 contractor effect Effects 0.000 description 2
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 description 2
- 229960001723 oxytocin Drugs 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 230000003191 uterotonic effect Effects 0.000 description 2
- 241000237519 Bivalvia Species 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- 208000005171 Dysmenorrhea Diseases 0.000 description 1
- 206010013935 Dysmenorrhoea Diseases 0.000 description 1
- RSEPBGGWRJCQGY-RBRWEJTLSA-N Estradiol valerate Chemical compound C1CC2=CC(O)=CC=C2[C@@H]2[C@@H]1[C@@H]1CC[C@H](OC(=O)CCCC)[C@@]1(C)CC2 RSEPBGGWRJCQGY-RBRWEJTLSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 206010029155 Nephropathy toxic Diseases 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229960004766 estradiol valerate Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229940041476 lactose 100 mg Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007694 nephrotoxicity Effects 0.000 description 1
- 231100000417 nephrotoxicity Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 238000005550 wet granulation Methods 0.000 description 1
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- Medicinal Preparation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a blood clam extract and application thereof in medicaments for promoting postpartum uterine contraction, wherein the blood clam extract is a water-soluble substance extracted from blood clam meat, has the effect of uterine contraction, and can be used for treating postpartum hemorrhage, incomplete uterine involution after drug flow surgery and induced abortion.
Description
Technical field
The present invention relates to marine active substance fiber crops blood clam extract for the preparation of the novelty teabag in uterotonic medicine.
Background technology
Fiber crops blood clam is commonly called as Scapharca subcrenata, numb clam, Mollusca, lamellibranchiata, Carnis Arca inflata order, Carnis Arca inflata section, and Scapharca subcrenata belongs to, and is mainly distributed in China, Korea and Japan coastal.Warm in nature, sweet in the mouth has enriches blood, the effects such as stomach invigorating." Bencao Jingshu " is recorded: " property is also nontoxic for wax, sweet in the mouth temperature, and sweet temperature can QI invigorating and invigorating middle warmer; then the five internal organs peace, and gastric qi is good for, the air-cooled all carbuncles of trusted subordinate's spinal column, and the strong then food of stomach is from disappearing; dirty warm then sun from, gas fill then blood from China ", but the effect of promotion uterine contraction has no report.
Uterus during Pregnancy increases gradually, uterine cavity capacity increase about 1000 times.Uterus weight increases nearly 20 times, in point puerperium, due to contraction, the retraction effect of uterus muscle, uterine volume is obviously reduced, the state before generally Zhou Shike returns to pregnancy in puerperal 5 ~ 6.If uterine contractions fatigue, more than, dripping unclean, the lower abdomen pain of lochia can be caused.Subinvolution of uterus, should take uterine contraction medicine, as oxytocin, and ergot fluid extract, ext leonuri sibirici liq; Easily there is uterine rupture in oxytocin, has cesarean history person to avoid use, multipara's (easily uterine rupture occurring) forbiddings more than 3 tires.Herba Leonuri can increase the weight of dysmenorrhea, cause puerperal uterine contraction pain, also has nephrotoxicity.Research and develop new promotion uterine contraction in puerperal, receive much concern in particular for Subinvoluted novel drugs in treatment puerperal, compare with traditional Herba Leonuri herein, prove that numb blood clam extract has the contraction effect of uterus of similar Herba Leonuri, there is good potential applicability in clinical practice.
Summary of the invention
The present inventor finds that numb blood clam extract has the effect of shrinking more by force uterus, can be used for promoting uterine contraction medicine in puerperal, in particular for treatment subinvolution in puerperal.
That is, the invention provides:
(1) numb blood clam extract promotes the purposes in uterine contraction medicine in puerperal in preparation, and wherein the preparation method of numb blood clam extract is as follows: 1. appropriate numb blood clam meat, homogenate; 2. add 1.5 times to the distilled water of volume, extract 25min in 30 DEG C of ultrasonic assistant; 3. 30 DEG C of stirring in water bath extract the centrifugal 5min of 2h, 4000r/min, get supernatant, and residue repeats aforesaid operations, merging filtrate, and after being evaporated to 1/3 volume, lyophilization obtains numb blood clam extract;
(2) according to the purposes of above-mentioned (1), it is characterized in that: numb blood clam extract is made capsule, tablet, pill, powder, granule together with pharmaceutically acceptable carrier;
(3) according to the purposes of above-mentioned (1), it is characterized in that: described uterine contraction refers to normal labor, Cesarean esction, uterine contraction after Artificial or drug-induced abortion.
Detailed description of the invention
By the following examples foregoing of the present invention is described in further detail again.But the present invention is not by the restriction of following embodiment.All technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1, preparation have the numb blood clam extract promoting uterine contraction effect in puerperal:
(1) appropriate numb blood clam meat, homogenate;
(2) add 1.5 times to the distilled water of volume, extract 25min in 30 DEG C of ultrasonic assistant;
(3) 30 DEG C of stirring in water bath extract the centrifugal 5min of 2h, 4000r/min, get supernatant, and residue repeats aforesaid operations, and merging filtrate, after being evaporated to 1/3 volume, lyophilization obtains numb blood clam extract.
Embodiment 2: numb blood clam extract is on the uterotonic impact of isolated mouse
The preparation of mice isolated uterine: unpregnancy female mice in experiment first 2 days, every day lumbar injection estradiol valerate, to improve the sensitivity of uterus to medicine; Cervical dislocation puts to death mice rapidly, and the uterine muscle strips being about 1.0cm is made in taking-up uterus of cutting open the belly.To put into the Magnus' bath filling 20ml with the uterus of silk thread ligation, by the silk thread in ligation uterus, one end is fixed on the specimen fixation hook in bath, and the other end is connected on tonotransducer, gives the load of uterus 1g.Start to record normal shrinkage curve after uterine contraction is steady.Each group is dripped respectively to the numb blood clam extract of Herba Leonuri aqueous solution 1 (in contrast), various concentration, after uterine contraction is steady, use Biolap420E
+biological Signal Collecting System record uterine myometrium moves, and the persistent period at the frequency of the uterine myometrium before and after the administration of each group record in 5min, amplitude and contraction interval, result is as following table.
The numb blood clam extract of table 1 on the impact of isolated uterine smooth muscle (
± S, n=12)
| Group | Frequency (point, secondary) | Amplitude (g) |
| Blank group | 3.4±0.7 | 0.56±0.16 |
| Herba Leonuri (0.1mg/ml) | 5.0±0.8 | 0.95±0.26 |
| Fiber crops blood clam extract (0.1mg/ml) | 4.0±1.3 | 0.71±0.10 |
| (0.3mg/ml) | 4.7±1.2 | 0.79±0.11 |
Embodiment 3: the preparation of numb blood clam extract tablet
Prescription:
Fiber crops blood clam extract 100mg
Lactose 100mg
Starch 295mg
Magnesium stearate 5mg
500mg altogether
Weigh each main component separately, numb blood clam extract is crossed the powder of 80 mesh sieves, lactose, starch mixes, and with wet granulation, dry, mix with magnesium stearate after integrating, tabletting, is pressed into the tablet of every sheet 500mg.
Embodiment 4: the preparation of numb blood clam extract capsule
Prescription:
Fiber crops blood clam extract 100mg
Starch 395mg
Magnesium stearate 5mg
500mg altogether
Weigh each main component separately, numb blood clam extract is crossed the powder of 80 mesh sieves, starch mixes, and filled capsules, makes the capsule of each pure heavy 500mg.
Claims (3)
1. numb blood clam extract and the application in promotion uterine contraction medicine in puerperal thereof, the preparation of this numb blood clam extract comprises the following steps:
(1) appropriate numb blood clam meat, homogenate;
(2) add 1.5 times to the distilled water of volume, extract 25min in 30 DEG C of ultrasonic assistant;
(3) 30 DEG C of stirring in water bath extract the centrifugal 5min of 2h, 4000r/min, get supernatant, and residue repeats aforesaid operations, and merging filtrate, after being evaporated to 1/3 volume, lyophilization obtains numb blood clam extract.
2. purposes according to claim 1, is characterized in that: numb blood clam extract is made capsule, tablet, pill, powder, granule together with pharmaceutically acceptable carrier.
3. purposes according to claim 1, is characterized in that: described uterine contraction refers to normal labor, Cesarean esction, uterine contraction after Artificial or drug-induced abortion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510183462.6A CN104825496A (en) | 2015-04-19 | 2015-04-19 | Scapharca granosa extract and application thereof in medicine for promoting postpartum uterine contraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510183462.6A CN104825496A (en) | 2015-04-19 | 2015-04-19 | Scapharca granosa extract and application thereof in medicine for promoting postpartum uterine contraction |
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| Publication Number | Publication Date |
|---|---|
| CN104825496A true CN104825496A (en) | 2015-08-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510183462.6A Pending CN104825496A (en) | 2015-04-19 | 2015-04-19 | Scapharca granosa extract and application thereof in medicine for promoting postpartum uterine contraction |
Country Status (1)
| Country | Link |
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| CN (1) | CN104825496A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103954704A (en) * | 2014-05-06 | 2014-07-30 | 济南康众医药科技开发有限公司 | Method for assaying content of blood clam protein |
| CN103969384A (en) * | 2014-05-06 | 2014-08-06 | 济南康众医药科技开发有限公司 | Content determination method of arca subcrenata hyperglycemia |
| CN103969367A (en) * | 2014-05-06 | 2014-08-06 | 济南康众医药科技开发有限公司 | Identification method of arca subcrenata protein |
| CN104434986A (en) * | 2014-12-29 | 2015-03-25 | 青岛大学 | Razor clam extractive used for promoting postpartum uterus contraction |
-
2015
- 2015-04-19 CN CN201510183462.6A patent/CN104825496A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103954704A (en) * | 2014-05-06 | 2014-07-30 | 济南康众医药科技开发有限公司 | Method for assaying content of blood clam protein |
| CN103969384A (en) * | 2014-05-06 | 2014-08-06 | 济南康众医药科技开发有限公司 | Content determination method of arca subcrenata hyperglycemia |
| CN103969367A (en) * | 2014-05-06 | 2014-08-06 | 济南康众医药科技开发有限公司 | Identification method of arca subcrenata protein |
| CN104434986A (en) * | 2014-12-29 | 2015-03-25 | 青岛大学 | Razor clam extractive used for promoting postpartum uterus contraction |
Non-Patent Citations (4)
| Title |
|---|
| YONGBO BAO等: "Polymorphism of the multiple hemoglobins in blood clam Tegillarca granosa and its association with disease resistance to Vibrio parahaemolyticus", 《FISH & SHELLFISH IMMUNOLOGY》 * |
| 李谦等: "毛蚶提取物生化性质初步分析", 《药物生物技术》 * |
| 赵光环等: "浅海毛蚶养殖技术", 《河北农业科技》 * |
| 钟梅等: "微量元素锌与子宫收缩力的关系", 《广东医学》 * |
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| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20150812 |