CN104823767A - Application of nativo to control over filial generations of brown planthopper - Google Patents
Application of nativo to control over filial generations of brown planthopper Download PDFInfo
- Publication number
- CN104823767A CN104823767A CN201510193508.2A CN201510193508A CN104823767A CN 104823767 A CN104823767 A CN 104823767A CN 201510193508 A CN201510193508 A CN 201510193508A CN 104823767 A CN104823767 A CN 104823767A
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- CN
- China
- Prior art keywords
- brown planthopper
- enemy
- water dispersible
- dispersible granules
- filial generations
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
Abstract
The invention relates to the technical field of biological control, in particular to application of nativo to control over filial generations of brown planthopper. Native water dispersible granule (with 75 % mass percent of active ingredients including 25 % mass percent of trifloxystrobin and 50% mass percent of tebuconazole) is utilized by the invention for control over filial generations of brown planthopper.The most salient feature of the invention is described in that nativo, as bactericide, helps to prevent of inhibit yeast-like endosymbiote from entering ovarian tissues from hemolymph of female adults of brown planthopper such that a genital system of brown planthopper is affected and the egg laying amount is reduced; and the hatching rate is greatly decreased because produced eggs lack symbiotic bacteria so that generation of the filial generations of brown planthopper is effectively controlled.
Description
Technical field
The present invention relates to technical field of biological control, particularly relate to and take the application of enemy surely in the filial generation of control brown planthopper.
Background technology
Brown planthopper is the important rice grub of Asia, and the primary pest in Ye Shi China Yangtze river basin, south China and southwestern rice district, causes high risks to China's Rice Production.Existing prevention and controls is mainly through promoting insect-proof rice kind or controlling brown planthopper by spraying insecticide.Insect-proof rice kind has certain control action to Population of Rice Brown Planthopper in a short time, but brown planthopper has very strong adaptability to pest-resistant rice varieties, and 3 generations of general breeding are energy adapted breed resistance just, thus needs to be aided with insecticide to prevent and treat brown planthopper.But, because a large amount of of chemical insecticide frequently make brown planthopper to which creating pesticide resistance, cause control efficiency obviously to decline.
There is a large amount of fungal component in brown planthopper fat-body, wherein, Yeast-like symbionts plays a part indispensable to growing of brown planthopper, and lacking the brown planthopper of Yeast-like symbionts can not normal development or survival.After brown planthopper sprouts wings, Yeast-like symbionts passes through brown planthopper ovary with ovum vertical transmission to filial generation brown planthopper.In vertical transmission process, Yeast-like symbionts dissociates to hemolymph from the fat-body of brown planthopper female adult pest, then enters ovary tissue, enters the egg mother cell of brown planthopper finally by endocytosis, thus completes the communication process from female generation to filial generation.In above-mentioned communication process, dissociate to the Yeast-like symbionts hemolymph from the fat-body of brown planthopper female adult pest, than the impact being subject to fungicide and other external factors easier in fat-body.
Since Yeast-like symbionts vertical transmission between brown planthopper generation, and Yeast-like symbionts and brown planthopper growth and breeding relation very tight, therefore, occur and reduce brown planthopper to cause harm to control filial generation brown planthopper by the means of " suppress or stop Yeast-like symbionts to enter the ovary of brown planthopper; the ovum making brown planthopper give birth to lacks Yeast-like symbionts; finally causing brown planthopper ovum to be difficult to hatch or filial generation brown planthopper is difficult to survive ", this will become the important new tool of the one of preventing and treating brown planthopper from now on.
Summary of the invention
The present invention is surely used for taking enemy the vertical transmission of Yeast-like symbionts between generation suppressed or stop in brown planthopper female adult pest body, thus reduces brown planthopper egg laying amount, egg hatching rate or make filial generation brown planthopper effectively control brown planthopper because scarce bacterium cannot survive.The present invention is that effective control of Brown Planthopper provides a kind of new thinking and countermeasure.
The object of the invention is to develop immunity to drugs to conventional chemical pesticides for brown planthopper, cause the deficiency that chemopreventive effects obviously declines, a kind of fungicide that utilizes is provided to take the vertical transmission of Yeast-like symbionts between generation in enemy's steady suppression brown planthopper female adult pest body, and then the new approaches causing filial generation brown planthopper cannot survive because lacking bacterium; Another object of the present invention there is provided a kind of new method of preventing and treating brown planthopper.
The object of the invention is achieved through the following technical solutions:
Take the steady water dispersible granules of enemy (active ingredient mass percent is 75 %, and wherein oxime bacterium ester mass percent is 25%, and Tebuconazole mass percent is 50%) for the control of brown planthopper filial generation fungicide.
Beneficial effect of the present invention: the invention provides and take the application of enemy surely in the filial generation of control brown planthopper.
Embodiment
Embodiment 1:(takes the preparation of the steady water dispersible granules of enemy)
Take the steady water dispersible granules of enemy, produced by Beyer Co., Ltd, will dissolve by the steady water dispersible granules running water of enemy, being made into final concentration is that 0.3g/L takes the steady preparation of enemy.
Embodiment 2:(takes the application of enemy surely in brown planthopper control)
Carry out according to the following steps:
(1) tillering and jointing stage TN1 rice plant, removes part root and base portion leaf sheath afterwash, naturally dries;
(2) compare with clear water, 0.04g/L Imidacloprid liquid is treated to positive control, and spraying final concentration is that 0.3g/L takes enemy steady, and require that liquid Pcnten-1 yne-4 is even, coverage rate is high, and amount of liquid medicine is suitable, does not produce loss and is advisable, naturally dry with moistening target surface;
(3) the rice seedling stem after step (2) process puts into the Boiling tube injecting 20ml paddy rice culture fluid in advance, each test tube rice seedlings, puberty consistent brown planthopper 1 nymph in age 1 is in vitro put into respectively at often propping up after 12 h, two-layer gauze sealing, each process repetition 150 times, after 1 h, observes invisible spectro nymph one by one, replace physically impaired polypide, ensure in vitro do not have because of physical damnification and the brown planthopper nymph of death.
(4) brown planthopper from 1 age nymph all temperature 26 ± 1 DEG C, humidity: 70-80%, light application time 16 h, interlunation 8 h the indoor cultivation of artificial climate.Observe every day and record the situation of growing of brown planthopper.The brown planthopper adult male and female of having sprouted wings in above-mentioned different disposal group are matched one by one, and corresponding access is equipped with in the Boiling tube of the rice seedling of its alignment processing group, two-layer gauze sealing.Observe the survival condition of adult every day, and change the fresh rice seedling through corresponding chemicals treatment, utilize binocular microscope to carry out microscopy to the rice seedling replaced out, record the index of correlation of laying eggs on the same day.After the rice seedling microscopy that adult stage changes, put it to new in vitro continuation and be cultured to pieces of an egg hatching, observe every day and add up hatching amount, incubation rate, hatching total amount etc.
(5) ovum getting just hatching carries out the mensuration of fungal component quantity, assay method is: 50, the brown planthopper ovum getting each processed group respectively under stereoscope, with the alcohol disinfecting of 75%, rinsed with sterile water, puts into 1.5ml centrifuge tube, adds the physiological saline 10 μ l of 0.9%, homogenate, then get 1 μ l homogenate drop on blood cell counting plate, close the lid fragmentation, under microscope (400 ×), add up fungal component quantity.
Embodiment 3:(takes the preventive and therapeutic effect opposed surely to brown planthopper nymph and adult)
Carry out according to the following steps:
(1) tillering and jointing stage TN1 rice plant, removes part root and base portion leaf sheath afterwash, naturally dries;
(2) compare with clear water, spraying final concentration is that 0.3g/L takes enemy steady, and require that liquid Pcnten-1 yne-4 is even, coverage rate is high, and amount of liquid medicine is suitable, does not produce loss and is advisable, naturally dry with moistening target surface;
(3) the rice seedling stem after step (2) process puts into the Boiling tube injecting 20ml paddy rice culture fluid in advance, often pipe 3 rice seedlings, access 3 brown planthopper nymph in age (or the brachypterism female adult pest of just having sprouted wings) 30, to spray the rice seedlings process after clear water as negative control, spray 0.04g/L Imidacloprid liquid and be treated to positive control, each process repeats for 3 times;
(4) within 4 days, brown planthopper lethality is added up after treatment.
Of the present invention the taking of test example 1:(opposes steady and conventional chemical pesticide control Piglet s colibacillosis)
Result of the test is in table 1.As can be seen from Table 1, enemy is taken surely obviously to inhibit brown planthopper egg laying amount, the quantity of ovum endosymbiosis bacterium and the incubation rate of ovum.
The steady impact on ovum fungal component quantity, egg hatching rate and egg laying amount of enemy taken by table 1
Test example 2:(takes the Piglet s colibacillosis opposing steady and conventional chemical pesticide control brown planthopper nymph and adult)
Result of the test is in table 2.As seen from Table 2, utilize by opposing the effect of steady control brown planthopper nymph and adult all be not as good as Imidacloprid.
The control efficiency of opposing surely to brown planthopper nymph and adult taken by table 2
Imidacloprid target is brown planthopper, and bactericide takes the steady target of enemy to be for Yeast-like symbionts in brown planthopper body, so take the effect of enemy steady control brown planthopper nymph and adult all be not as strong as Imidacloprid.But after taking the steady process of enemy, brown planthopper ovum endosymbiosis bacterium quantity, egg hatching rate and average egg laying amount all significantly decline, and effect is stronger than Imidacloprid, effectively improves the control efficiency of filial generation brown planthopper.Main cause is that bactericide is taken enemy surely to stop or inhibits Yeast-like symbionts to enter ovary tissue from the hemolymph of brown planthopper female adult pest, thus make brown planthopper genital system influenced, egg laying amount reduces, the ovum of output also causes incubation rate significantly to reduce because lacking fungal component, thus effectively controls the generation in brown planthopper time generation.
Use control efficiency of taking enemy surely effectively can improve filial generation brown planthopper of the present invention.The prevention and controls of relative popularization insect-proof rice kind and use of insecticide control brown planthopper, brown planthopper of the present invention prevents and treats the problem that new method effectively can avoid brown planthopper water resistant rice varieties and anti-insecticide; The fungicide that the present invention uses not directly to brown planthopper generation effect, but passes through the Yeast-like symbionts prevention and control brown planthopper indirectly in its body, thus safer to people, animal, fish, beneficial insect etc.
Claims (3)
1. take the application of enemy surely in the filial generation of control brown planthopper.
2. new method according to claim 1, obtained by following methods: be used for stoping or suppress Yeast-like symbionts to enter ovary tissue from the hemolymph of brown planthopper female adult pest by taking the steady water dispersible granules of enemy, thus make brown planthopper genital system influenced, egg laying amount reduces, the ovum of output also causes incubation rate significantly to reduce because lacking fungal component, thus effectively controls the generation in brown planthopper time generation; Take the steady water dispersible granules of enemy to be produced by Beyer Co., Ltd, described active ingredient mass concentration of taking the steady water dispersible granules of enemy is 75%, and wherein oxime bacterium ester accounts for 25%, and Tebuconazole accounts for 50%.
3. the steady water dispersible granules running water of enemy of taking according to claim 2 dissolves, and concentration is 0.3g/L-0.6g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510193508.2A CN104823767A (en) | 2015-04-23 | 2015-04-23 | Application of nativo to control over filial generations of brown planthopper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510193508.2A CN104823767A (en) | 2015-04-23 | 2015-04-23 | Application of nativo to control over filial generations of brown planthopper |
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|---|---|
| CN104823767A true CN104823767A (en) | 2015-08-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510193508.2A Pending CN104823767A (en) | 2015-04-23 | 2015-04-23 | Application of nativo to control over filial generations of brown planthopper |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532581A (en) * | 2015-12-16 | 2016-05-04 | 中国计量学院 | In-vitro study method of insect symbiotic bacterium transovarial vertical transmission |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918702A (en) * | 2014-04-25 | 2014-07-16 | 中国计量学院 | Application of mixed formula preparation for controlling brown paddy plant hopper |
| CN103918703A (en) * | 2014-04-25 | 2014-07-16 | 中国计量学院 | Mixed formula preparation for controlling brown paddy plant hopper |
| CN103918701A (en) * | 2014-04-25 | 2014-07-16 | 中国计量学院 | Preparation method of Nadiwen-imidacloprid mixed formula preparation |
-
2015
- 2015-04-23 CN CN201510193508.2A patent/CN104823767A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918702A (en) * | 2014-04-25 | 2014-07-16 | 中国计量学院 | Application of mixed formula preparation for controlling brown paddy plant hopper |
| CN103918703A (en) * | 2014-04-25 | 2014-07-16 | 中国计量学院 | Mixed formula preparation for controlling brown paddy plant hopper |
| CN103918701A (en) * | 2014-04-25 | 2014-07-16 | 中国计量学院 | Preparation method of Nadiwen-imidacloprid mixed formula preparation |
Non-Patent Citations (4)
| Title |
|---|
| 吕仲贤等: "共生菌在褐飞虱致害性变化中的作用", 《昆虫学报》 * |
| 吕仲贤等: "共生菌对褐飞虱生长发育和生殖的影响", 《植物保护学报》 * |
| 白旭等: "类酵母共生菌与灰飞虱卵巢发育和产卵量的关系", 《植物保护学报》 * |
| 陈列忠等: "共生菌在褐飞虱防治中的应用", 《农药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532581A (en) * | 2015-12-16 | 2016-05-04 | 中国计量学院 | In-vitro study method of insect symbiotic bacterium transovarial vertical transmission |
| CN105532581B (en) * | 2015-12-16 | 2019-04-05 | 中国计量大学 | A method of insect fungal component is studied in vitro through ovum vertical transmission |
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Application publication date: 20150812 |
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