CN101779653B - Formulated pesticide for preventing black streaked dwarf of rice - Google Patents
Formulated pesticide for preventing black streaked dwarf of rice Download PDFInfo
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Abstract
The invention belongs to the field of pesticide technology and relates to a formulated pesticide for preventing black streaked dwarf of rice; the effective ingredients of the formulated pesticide are moroxydine hydrochloride, ribavirin and zinc citrate; the formulated pesticide for preventing black streaked dwarf of rice has the advantages that: the prevention and control effect is good with more than 90 percent; the product which is used scientifically and rationally is safe for rice, the water solubility is good, the quick acting effect is good, the formulated pesticide can prevent the black streaked dwarf which has resistance to the existing medicines, the formulated pesticide has no adverse effect to beneficial living beings and has no toxicity to people and livestock.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of Recompounded pesticide of preventing and treating black streaked dwarf virus of rice.
Background technology
In the real process of agricultural production, various disease conditions also can occur in plant, black streaked dwarf virus of rice (Ricestipe virus is called for short RSV) is a kind of rice disease that is caused by virus, generation is all arranged, serious harm paddy quality and output in each paddy rice producing region, the whole nation.Because black streaked dwarf virus of rice is also thought the cancer of paddy rice by a lot of experts, according to the plant protection department statistics, black streaked dwarf virus of rice account for paddy rice due to illness the insect pest underproduction 43.63%.Black streaked dwarf virus of rice is a kind of novel very strong chronic disease of pesticide resistance, affects for a long time the output of crops, and this has just required corresponding agricultural chemicals to control black streaked dwarf virus of rice, improves actual control efficiency, thereby improves rice yield.
Summary of the invention
The object of the present invention is to provide a kind of Recompounded pesticide of preventing and treating black streaked dwarf virus of rice, control efficiency is good.
The present invention is by the following technical solutions:
A kind of Recompounded pesticide of preventing and treating black streaked dwarf virus of rice, the active ingredient of this Recompounded pesticide are Moroxydine Hydrochloride, ribavirin and zinc citrate.
Described Recompounded pesticide comprises that mass fraction is the Moroxydine Hydrochloride of 10-50 part, the ribavirin of 0.5-1 part and the zinc citrate of 1-2 part.
Described Recompounded pesticide comprises that mass fraction is the Moroxydine Hydrochloride of 20-30 part, the ribavirin of 0.5-1 part and the zinc citrate of 1-2 part.
Described Recompounded pesticide comprises that mass fraction is 30 parts Moroxydine Hydrochloride, 1 part ribavirin and 2 parts zinc citrate.
Described Recompounded pesticide is added with filler and the auxiliary agent of following mass fraction, and described auxiliary agent is that 0.5-1 part kinase, 3-5 part are pulled open powder, 3-5 part sodium lignin sulfonate and 5-10 part wetting agent; Described filler is 10-20 part potassium dihydrogen phosphate, 5-10 part sodium nitrate and 10-16 part bacillus licheniformis offcuts.
Described Recompounded pesticide is added with filler and the auxiliary agent of following mass fraction, and described auxiliary agent is that 1 part of kinase, 5 parts pull open powder, 5 parts of sodium lignin sulfonates and 10 parts of wetting agents; Described filler is 20 parts of potassium dihydrogen phosphates, 10 parts of sodium nitrate and 16 parts of bacillus licheniformis offcuts.
Described kinase is coacetylase, and described wetting agent is ammonium sulfate.
The preparation method of described bacillus licheniformis offcuts is that the filter residue of bacillus licheniformis after fermentation, washing, crystallization, filtration forms through drying, pulverizing.
Preparation method of the present invention gets final product for each raw material mixes after pulverizing.
The using method of Recompounded pesticide of the present invention is watered by Recompounded pesticide and is diluted to 800-1000 doubly, and foliage-spray, formulation rate are per hectare 450-750 gram Recompounded pesticide, and the active ingredient dosage is 148.5-247.5 g/ha.
The black streaked dwarf virus of rice main cause is a kind of virus disease that small brown rice planthopper or soil severe contamination cause, in case black-streaked dwarf virus occur then occur: Lao Ye blackout, embrittlement, harden, core leaf hai roll, shrinkage, not long, the bottom secondary root is undeveloped or without giving birth to secondary root, the dead dead seedling of seedling is not eared; What the present invention be directed to the research and development of this disease has treatment, roots out the Recompounded pesticide with prevention effect, the soluble powder take Moroxydine Hydrochloride, ribavirin and zinc citrate as active ingredient.Wherein, Moroxydine Hydrochloride mainly suppresses rice black-streaked dwarf virus albumen and generates, virus can not be invaded and copy, ribavirin mainly destroys the germ nucleosides and forms, and to the virus of having invaded it is solidified, and suppresses conidial formation on the scab, interior absorption is strong and can Bidirectional Conduction, in the new blade that grows after the liquid energy transporting dispenser that rice leaf absorbs, the medicament of rice root absorption can transporting arrive blade and cob part, thus the performance preventing disease theraping effect; Zinc citrate is that the part of polyphenol enzyme is (with zinc citrate and without zinc sulphate, the excessive easy generation poisoning zinc citrate of zinc sulphate then not can), potassium dihydrogen phosphate in the filler is the requisite nutrient components of crops, kinase in the auxiliary agent is that coacetylase is the constituent of primary juice, thereby particularly paddy rice is had take root, carry out photosynthetic, make chlorophyll, promote primary juice to flow, make the effect that it grows young leaves, the heading of loosing core can make virus obtain effecting a permanent cure when treatment virus, be used for thoroughly rooting out and preventing of black streaked dwarf virus of rice.
The present invention seeks a kind of medicament that can effectively root out, prevent and treat black streaked dwarf virus of rice, now that Moroxydine Hydrochloride, ribavirin and zinc citrate is composite, and filter out the best compound proportion of its synergistic effect, thereby effectively prevent and treat the generation of black streaked dwarf virus of rice; Contain a large amount of bacillus licheniformis active ingredient in the bacillus licheniformis offcuts among the present invention, belong to biological bactericide, can suppress the growth and breeding of germ.The Recompounded pesticide exploitation that the present invention prevents and treats black streaked dwarf virus of rice has solved a great problem with research for plant protection, by use this product in paddy rice, its drug effect of verification and economic benefit provide scientific basis for the large scale application of this product, welcome by the rice farming, obtained significant economic benefit and social benefit.
The Recompounded pesticide of preventing and treating black streaked dwarf virus of rice of the present invention has the following advantages:
(1) control efficiency is good, and preventive effect can reach 90.34%～95.41%; (2) this product of scientific and reasonable use is to rice safety; Seedling stage, transplanting date, heading stage, blooming stage all can use; (3) good water solubility, quick-acting is good, can prevent and treat the black streak dwarf that existing medicament is had resistance; (4) the field beneficial organism is had no harmful effect, to people and animals' low toxicity.
Embodiment
Embodiment 1
The composition of Recompounded pesticide is: 50 parts Moroxydine Hydrochloride, 1 part ribavirin, 2 parts zinc citrate, 1 part of coacetylase, 5 parts pull open powder, 5 parts of sodium lignin sulfonates and 10 parts of ammonium sulfate, 20 parts of potassium dihydrogen phosphates, 10 parts of sodium nitrate and 16 parts of bacillus licheniformis offcuts.
Embodiment 2
The composition of Recompounded pesticide is: 10 parts Moroxydine Hydrochloride, 0.5 part ribavirin, 1 part zinc citrate, 0.5 part of coacetylase, 3 parts pull open powder, 3 parts of sodium lignin sulfonates and 5 parts of ammonium sulfate, 10 parts of potassium dihydrogen phosphates, 5 parts of sodium nitrate and 10 parts of bacillus licheniformis offcuts.
Embodiment 3
The composition of Recompounded pesticide is: 20 parts Moroxydine Hydrochloride, 0.8 part ribavirin, 1.5 parts zinc citrate, 0.8 part of coacetylase, 4 parts pull open powder, 4 parts of sodium lignin sulfonates and 8 parts of ammonium sulfate, 15 parts of potassium dihydrogen phosphates, 8 parts of sodium nitrate and 12 parts of bacillus licheniformis offcuts.
Embodiment 4
The composition of Recompounded pesticide is: 30 parts Moroxydine Hydrochloride, 1 part ribavirin, 2 parts zinc citrate, 1 part of coacetylase, 5 parts pull open powder, 5 parts of sodium lignin sulfonates and 10 parts of ammonium sulfate, 20 parts of potassium dihydrogen phosphates, 10 parts of sodium nitrate and 16 parts of bacillus licheniformis offcuts.
Umber among the above embodiment is mass fraction, and the preparation method of the bacillus licheniformis offcuts among the above embodiment is that the filter residue of bacillus licheniformis after fermentation, washing pili, water lotion crystallization, crystalline mixture filter forms through drying, pulverizing.
Below prevent and treat the test portion of the Recompounded pesticide of black streaked dwarf virus of rice for the present invention:
Field experiment report as an example of embodiment 4 Recompounded pesticides example:
1, demonstration test
1.1 demonstration place and basic condition: demonstration test is chosen in Sang Cun administrative village, Hua Xiansangcun township, Henan Province demonstration paddy field.Soil is two to close class soil, and organic 1.58%, PH7.1 is the higher Rice Production plot of fertility.
1.2 rice varieties and planting type: for showing that (examination) paddy rice is " No. 1, ruddiness " each residential quarter field conventional cultivation management.
1.3 the method to set up of demonstration test: demonstration is chosen in flakes 50 mu of paddy rice plot of equal cultivation management condition, in the black streaked dwarf virus of rice their early stage, even sprayed 2 times every 10 days, by every mu of 30kg clear water, process I: add 30g embodiment 4 Recompounded pesticides by every mu of 30kg clear water; Process II: add 40g embodiment 4 Recompounded pesticides by every mu of 30kg clear water; Process III: add 50g embodiment 4 Recompounded pesticides by every mu of 30kg clear water; The foliage-spray with each residential quarter of time is carried out in three kinds of processing, and carries out the paired observation of demonstration test.
2, demonstration test results and analysis
2.1 result of the test can find out, along with Enrichment, embodiment 4 Recompounded pesticides obviously increase the preventive effect of black streaked dwarf virus of rice, see the following form 1.
Table 1 embodiment 4 Recompounded pesticides are prevented and treated the black streaked dwarf virus of rice result
| Treatment agent | Effective ingredient consumption (g/ha) | Preventive effect after the last dispenser (%) (respectively repeating mean value) |
| Process I | 148.5 | 80.5 |
| Process II | 198 | 86.9 |
| Process III | 247.5 | 92.3 |
| Clear water | / | / |
2.2 show by experimental observation, embodiment 4 Recompounded pesticides are to rice safety, no side effects occurs.
3, conclusion
" No. 1, ruddiness " is on the identical basis of field management measure, foliage-spray embodiment 4 Recompounded pesticides begin dispenser in the first period of disease of paddy rice black streaked dwarf virus of rice, spray again 1 time after 10 days paddy rice black streaked dwarf virus of rice disease had good control efficiency, adopt embodiment 4 Recompounded pesticide 148.5-247.5 gram (per hectare effective ingredient consumption) continuous administration 2 times, 10 days afterwards average preventive effect can reach 92.3% effect, and to rice safety.
4, overall merit
Show through the demonstration test operating position: (1) control efficiency is good, and preventive effect can reach 95%; (2) this product of scientific and reasonable use is to rice safety; (3) water soluble quick-acting is good, can prevent and treat the black streaked dwarf virus of rice that existing medicament is had resistance; (4) the field beneficial organism is had no harmful effect, to people and animals' low toxicity.
The ecotoxicology data of embodiment 4 Recompounded pesticides
1 experiment material
1.1 reagent agent
Embodiment 4 Recompounded pesticides
1.2 biological for examination
1.2.1 for the examination honeybee
Apis mellifera (Apis mellifera L.), the worker bee that grows up, animal science institute of Zhejiang University provides.
1.2.2 for the examination birds
Japanese quail (Coturnix coturnix japonica), Hangzhou Xiaoshan city proper and areas just outside its gates town fowl and livestock farm provides.Selecting the length of time is that 30 days, the quail of bouncing are for examination.Before the test under the laboratory test condition one week of precuring, during the precuring quail all growth is normal, without illness without death.
1.2.3 for the examination fish
The test fingerling is zebra fish (rachydanio rerio), average body long (connecting tail) 3.0cm, and average weight is 0.180g approximately.Provided by the aquarium of setting sail, Zhejiang.Raise and train a week indoor before the test, zebra fish grows normally during raising and train, and without dead, without illness, all health is big or small basically identical.Test stops feeding the previous day, and duration of test is feeding not.
1.2.4 for the examination silkworm
Supplying the examination silkworm egg is autumn wind * white jade, plays silkworm two ages for examination, and silkworm egg is provided by Zhejiang Province's silkworm egg company.
2 experimental instrument and equipments
2.1 test peak cage: 500ml glass beaker (sealing with gauze);
2.2 test birdcage: plastic-coated iron cage, long 50cm, wide 35cm, high 35cm, two of band feed box are given one of water tumbler;
2.3 test fish jar: clear glass cylinder, internal diameter 30cm, high 30cm;
2.4 other: 1ml syringe, 16# gavage pin, volumetric flask, beaker, thermometer, pipette, liquid-transfering gun, 5ml small beaker, absorbent cotton, pH meter, dissolved oxygen meter, artificial climate incubator etc.
3 test methods
3.1 honeybee test
Adopt the absorption method to investigate the absorption toxicity of medicament.
Refined sugar solution with 50% is mixed with embodiment 4 Recompounded pesticides the liquid of a plurality of variable concentrations for examination.Get the liquid 5ml for preparing and move into and be equipped with in the 5ml small beaker of absorbent cotton, again small beaker is put in the 500ml large beaker and takes food for honeybee.
In large beaker, move into 20 adult workers bee, seal with gauze.Each processes triplicate, establishes blank.Test is carried out under poor light condition, 25 ± 1 ℃ of environmental temperatures, and relative moisture 70 ± 5% is after the chemicals treatment 24, the 48h investigation situation of being poisoned to death.
3.2 birds test
25 ± 2 ℃ of test temperatures, the room ventilation printing opacity.Feed adopts quail feed, feeds with clear water simultaneously.Test quail 10 every group (male and female half and half) is as a repetition.Front fasting 12-20 hour of test is only for clear water.Embodiment 4 Recompounded pesticides with distilled water diluting after for the examination, determine the toxicity test scope by prerun.Formal test arranges some groups of variable concentrations liquids, and other establishes one group and fills with clear water as blank, and 1 repetition is established in each processing.The per os contamination administration by gavage that test adopts disposable dosage directly to inject.With the liquid of variable concentrations by the processing of contaminating of the dosage of every 100g body weight quail perfusion 1ml.Quail after the processing is raised according to a conventional method, the poisoning symptom of observed and recorded quail and death figure.Test lasts 7 days, and the death figure accumulation is calculated.Calculate medicament to the medial lethal dose LD of quail with the DPS data processing platform (DPP)
50Value and 95% confidence limit CL
0.95.
3.3 pilot fish
Adopt static method.
Laboratory temperature is controlled at 22 ± 1 ℃. and test water deposits and aeration, test are raised and train and tested all with fish and carry out in the laboratory.Test water is running water, and more than 24 hours, hardness 86mg/L is (with CaCO through the gas disposal of aeration dechlorination
3Meter).Test chamber is positioned in the Water Tank with Temp.-controlled of continuous-flow type device, 22 ± 1 ℃ of water temperatures. the confession reagent liquid of 10 liters of variable concentrations of preparation in each test cylinder (jar), and drop into 10 of zebra fishs.Determine the toxicity test concentration range by preliminary experiment.Formal test arranges the liquid of some groups of variable concentrations, and other establishes one group of blank, and 3 repetitions are established in each processing.Regularly measure dissolved oxygen and the pH value of test solution in each cylinder in the experiment.Test lasts 96 hours, Continuous Observation record 24,48,72,96h zebra fish poisoning symptom and death figure, in time remove dead fish.Test is processed and is adopted the DPS data processing platform (DPP) to calculate medicament to the half lethal concentration (LC of zebra fish
50) value.
3.4 silkworm test
Embodiment 4 Recompounded pesticides are mixed with the liquid of several variable concentrations with deionized water.With the liquid dipping 10g mulberry leaf of variable concentrations, dip time is 10 seconds, for nibbling, moves in every diameter 15cm culture dish and plays silkworm, triplicate 20 two ages behind liquid natural air drying on the mulberry leaf.If blank.
Test is carried out under poor light condition, 25 ± 1 ℃ of temperature, and relative moisture 70 ± 5% is after processing 24,48,96h to three plays the poisoning symptom of tested silkworm and death condition age.Experiment is carried out data statistics to data after finishing.
4 experimental results and toxicity assessment
4.1 honeybee test
The contrast honeybee is movable and take food normal.The calomel poisoning honeybee is relatively more excited, constantly creeps, and easily falls on the clump wall of cup; Serious poisoning honeybee is more weak, or struggles after the rollover, and some digitus and feeler are slowly brandished, and what have constantly smoothes out with the fingers rafter with front foot.Dead honeybee wing opens, and the leg purse up colludes Fu, Young-locust-without-wings and stretches out.The lethality of test liquor strength and each group honeybee sees Table 2, and statistics sees Table 3.
Table 2 embodiment 4 Recompounded pesticides are to the absorption toxicity test result of honeybee
Table 3 embodiment 4 Recompounded pesticides are to the lethal concentration of 50 of honeybee
According to " chemical pesticide environmental safety assessment test rule " to honeybee toxicity grading standard; LC
50Value≤0.mg/L's is highly toxic pesticide; 0.5mg/L≤LC
50＜20mg/L's is the height agricultural chemicals; 20mg/L＜LC
50≤ 200mg/L's is medium toxic pesticides; LC
50＞00mg/L is low-toxin farm chemicals.Experimental result shows, embodiment 4 Recompounded pesticides are to the absorption toxicity LC of honeybee
50Being 3.4053mg/L when 48h, is high poison to honeybee toxicity.
4.2 birds test
The quail growth normally occurs without dead in the contrast of experimentation empty.
In 8 hours, tested quail occurs without dead without obvious poisoning symptom after the administration; Administration is observed after processing 24h, and without newly-increased dead, quail is movable normal; Within the follow-up observation period, occur without new death.Test liquor strength and experimental result see Table 4, and statistics sees Table 5.
Table 4 embodiment 4 Recompounded pesticides are to the absorption toxicity test result of quail
Table 5 embodiment 4 Recompounded pesticides are to the lethal dose of 50 (LD of quail
50)
According to " chemical pesticide environmental safety assessment test rule " the toxic grade criteria for classifying to bird: LD
50≤ 10mg/kg is highly toxic pesticide; 10mg/kg＜LD
50≤ 500mg/kg is medium toxic pesticides; LD
50＞500mg/kg is low-toxin farm chemicals.This test shows that embodiment 4 Recompounded pesticides are low toxicity to the toxicity of birds.
4.3 pilot fish
Behind the contact liquid, substantially without poisoning symptom.The lethality of test liquor strength and each group zebra fish sees Table 6, and statistics sees Table 7.
Table 6 embodiment 4 Recompounded pesticides are to the acute toxicity testing result of zebra fish
Table 7 embodiment 4 Recompounded pesticides are to the lethal concentration of 50 (LD of zebra fish
50)
According to " chemical pesticide environmental safety assessment test rule " to the toxicity in fish grading standard; LC
50≤ 0.1mg/L's is highly toxic pesticide; 0.1mg/L＜LC
50≤ 1.0mg/L's is high-toxic pesticide; 1.0mg/L＜LC
50≤ 10.0mg/L's is medium toxic pesticides; LC
50＞10.0mg/L is low-toxin farm chemicals.Experimental result shows, embodiment 4 Recompounded pesticides are to 96 hours LC of zebra fish
50＞1000mg/L is therefore the toxicity of embodiment 4 Recompounded pesticides belongs to low toxicity.
4.4 silkworm test
Test the different time observed result and show, the contrast silkworm normally takes food or creeps, and 96h is all casted off a skin and become for three ages after the experiment beginning.24h and contrast are substantially without difference; The 48h calomel poisoning contrasts the silkworm body little; The 96h calomel poisoning, the silkworm individuality is less, and the Silkworm, Bombyx mori of seriously poisoning is stood transfixed to the ground, and has body fluid to ooze out on every side, and dead Silkworm, Bombyx mori shortens, blackout.Test liquor strength and experimental result see Table 8, and statistics sees Table 9.
Table 8 embodiment 4 Recompounded pesticides are to the acute toxicity testing result of silkworm
Table 9 embodiment 4 Recompounded pesticides are to the lethal concentration of 50 (LD of zebra fish
50)
According to " chemical pesticide environmental safety assessment test rule " to the silkworm class toxic grade criteria for classifying; LC
50≤ 0.5mg/L's is highly toxic pesticide; 0.5mg/L＜LC
50≤ 20mg/L's is high-toxic pesticide; 20mg/L＜LC
50≤ 200mg/L's is medium toxic pesticides; LC
50＞200mg/L is low-toxin farm chemicals.Embodiment 4 Recompounded pesticides LC during to the 96h of silkworm
50＞982mg/L belongs to low toxicity to the toxicity of silkworm.
The toxicity test of four kinds of environmental organisms such as honeybee, quail, fish silkworm has been carried out in above experiment to embodiment 4 Recompounded pesticides, for this agriculture chemical registration provides the ecotoxicology data.Measurement result is as follows:
Honeybee LC
50(48h) 3.4053mg/L
Quail LC
50(7d)＞1000mg/Kg
Zebra fish LC
50(96d)＞1000mg/L
Silkworm LC
50(two ages, 96d)＞982mg/L
According to toxicity and the risk grade scale of agricultural chemicals to environmental organism, embodiment 4 Recompounded pesticides are high poison to honeybee; Be low toxicity to bird; Be low toxicity to fish; Be low toxicity to silkworm.
Embodiment 1 Recompounded pesticide field experiment report:
Field trial draws in the Wuzhi County, Henan, prevent and treat black streaked dwarf virus of rice with embodiment 1 Recompounded pesticide and have good control efficiency, 37-50 g/acre of even spraying, medicine is black streak dwarf decline rate 92.35% one day after, three days black streak dwarf decline rates 95.29% behind the medicine, seven days black streak dwarf decline rates 93.33% behind the medicine.
The field experiment report of embodiment 3 Recompounded pesticides:
Also carried out the spread demonstration test in Zhejiang, this time test minute three processing, spraying medicine concentration is respectively 35 g/acres, 42.5 g/acres, 50 g/acres, wherein 10 days black streak dwarf decline rate average out tos more than 90% behind 35 g/acres of dispenser rice field medicines.
Meanwhile, also carried out the spread demonstration test at Jining of Shandong Province, this time test minute three processing, spraying medicine concentration is respectively 35 g/acres, 42.5 g/acres, 50 g/acres, wherein 35 g/acres of dispenser rice field medicines one day after the black streak dwarf decline rate reach 92.54%, three days black streak dwarf decline rates 96.38% behind the medicine, seven days banded sclerotial blight decline rates 92.66% behind the medicine.
The formulation screening test of Recompounded pesticide of the present invention
Black streaked dwarf virus of rice is a kind of rice disease that has virus to cause, generation is all arranged, serious harm rice yield and quality in each rice belt, the whole nation.Now that Moroxydine Hydrochloride, ribavirin and zinc citrate is composite, and filter out its control, the best compound proportion of synergistic effect.
One, materials and methods
1, reagent agent
The former medicine of ribavirin and Moroxydine Hydrochloride and zinc citrate is commercially available, and its different proportioning mixtures are prepared voluntarily by the applicant.
2, for the examination material
For trying the water rice varieties for the susceptible force of black-streaked dwarf is educated japonica rice No. 3, be with malicious small brown rice planthopper available from insect teaching and research room of Agricultural University Of He'nan.
3, experiment equipment
Electronic balance (sensibility reciprocal 0.1mg), beaker, volumetric flask, glass rod, pipette, polypots, gauze.
4, test method
4.1 medicament preparation
With sterile water Moroxydine Hydrochloride is made into the 1000mg/L mother liquor, and dilutes 100,50,25,12.5 and 6.25 times, be mixed with concentration and be 10,20,40,80 and 160mg/L Moroxydine Hydrochloride series mass gradient solution for examination.
Adopting the compound concentration that uses the same method is the mother liquor of 100mg/L ribavirin, and with sterile water be mixed with concentration be 0.5,1,2,4 and 8mg/L ribavirin series mass gradient solution for the examination.
Adopting the compound concentration that uses the same method is the mother liquor of 100mg/L zinc citrate, and with sterile water be mixed with concentration be 1,2,3,4 and 8mg/L zinc citrate series mass gradient solution for the examination.
Respectively get an amount of volume mother liquor, be that the ratio of 10: 1: 2 (proportioning 1), 20: 1: 2 (proportioning 2), 30: 1: 2 (proportioning 3), 40: 1: 2 (proportioning 4) and 50: 1: 2 (proportioning 5) is mixed in mass ratio with Moroxydine Hydrochloride and ribavirin and zinc citrate, and according to pre-test result, with sterile water each assembly is supplied examination than the quality gradient solution that is diluted to variable concentrations:
Proportioning 1 moroxydine: ribavirin: zinc citrate=10: 1: 2 2.5,5,10,20,40mg/L
Proportioning 2 moroxydines: ribavirin: zinc citrate=20: 1: 2 2.5,5,10,20,40mg/L
Proportioning 3 moroxydines: ribavirin: zinc citrate=30: 1: 2 2.5,5,10,20,40mg/L
Proportioning 4 moroxydines: ribavirin: zinc citrate=40: 1: 25,10,20,40,80mg/L
Proportioning 5 moroxydines: ribavirin: zinc citrate=50: 1: 25,10,20,40,80mg/L;
4.2 test method
Adopt pot-culture method.Rice paddy seed is with the screening of 20%NaCl aqueous solution soaking, and with after the clear water flushing 5 times, seed soaking is 2 days under the room temperature again, and the moisturizing vernalization 1 day that drains away the water in 35 ℃ of incubators is seeded in the polypots that diameter is about 15cm 25 of every alms bowl sowings after seed shows money or valuables one carries unintentionally.Just grown to 2 leaves to rice seedlings and rejected weak seedling during the phase, every alms bowl keeps the rice seedling of 15 strain growing way homogeneous, when most of rice seedling grows to 3 leaves, 1 heart, with being with malicious plant hopper Inoculated Rice black-streaked dwarf virus.24h carries out even spraying with the medicament for preparing to the rice leaf positive and negative and processes before the inoculation, and is fully moistening but not drip be degree take the blade face.Every processing repeats 3 times, and 1 reprocessing 15 strain is 1 pot culture.Medicament sprays complete, and every alms bowl covers respectively translucent cover, and seal with gauze on the top, and by the quantity of 5 of every young plants, access 2-3 is with malicious small brown rice planthopper age, every 6h left and right sides disturbance gauze once, makes tested seedling evenly be subjected to poison.Behind the 48h, small brown rice planthopper is all removed.After inoculating for 3 weeks, the control treatment state of an illness tends towards stability, and reinvestigates the black streaked dwarf virus of rice incidence.
5, investigation and statistical method
Investigation morbidity strain number and classification are recorded all and are processed paddy rice state of an illness a situation arises, calculate disease index and inhibition, adopt the DPS data handling system to calculate the EC of each medicament
50And EC
90And 95% confidence limit.
Black streaked dwarf virus of rice severity Scaling standard is as follows:
5 grades: growing way is very poor, state of an illness full wafer or part blackout, obfuscation, embrittlement, hardens, new leaf rolling, the withered core of shrinkage, withered, do not ear; Root is without secondary root.
4 grades: growing way is very poor, and sick leaf obfuscation is green, and the dark yellow leaf disease spot of bottom leaf is continuous, random but black not hard, and upper leaf has dark green symptom, and black an IOU issued by a post office shape spot is large and many; The root secondary root is undeveloped.
3 grades: growing way is very poor, but sick leaf is black slightly dark, and the dark green scab of lower part is continuous, random, and spot enlarges, and upper leaf has dark yellow symptom, and the jaundice of core leaf is wrinkle slightly; The root secondary root is less.
2 grades: growing way a little less than, sick leaf is slightly dark, the core leaf roll is bent, sick section is slightly dark yellow, is fragmentary point-like or black dull strip, sick strong intersection is obviously clear, the root growth Non Apparent Abnormality;
1 grade: growing way slightly a little less than, fragmentary point-like is dark green, there is the interval in sick strong section, sick leaf is slightly dark along vein, spot seldom, root growth is normal;
0 grade: without scab, growing way is fine, and the initial stage, visible very little scab was covered up with the growth of seedling.
Computing formula:
Calculate the coefficient of synergism SR of mixture according to the Wadely method, judge the synergistic effect of different proportionings with synergy ratio (SR), SR＜0.5 is antagonism, and SR=0.5-1.5 is summation action, and SR＞1.5 are synergistic effect.
The correlation computations formula is as follows:
A, B are the compositions of each bactericide in the formula, and a, b are the ratios of these compositions in mixture.
6, results and analysis
After 3 weeks, the incidence of black streaked dwarf virus of rice after investigation Moroxydine Hydrochloride, ribavirin, zinc citrate different proportion Mixed Pharmacy are processed, experimental result is as shown in table 10:
The incidence of black streaked dwarf virus of rice after the different chemicals treatment of table 10
Utilize the DPSa data handling system to calculate the EC of each medicament
50, EC
90With 95% confidence interval (CL
0.95), and calculate the synergy ratio (SR) of five assembly ratio by Wadely, experimental result such as table 11:
Table 11 Moroxydine Hydrochloride and ribavirin and zinc citrate are to the formulation screening test result of black streaked dwarf virus of rice bacterium
| Medicament | Regression equation | Correlation coefficient | EC50 | CL0.95 | EC90 | CL0.95 | SR |
| Moroxydine Hydrochloride | Y＝2.9883+1.2716x | 0.9524 | 37.15 | 20.77-54.50 | 388.88 | 158.57-959.69 | |
| Ribavirin | Y＝4.4504+1.4679x | 0.9789 | 2.39 | 1.87-2.30 | 17.68 | 10.06-31.07 | |
| Zinc citrate | Y＝4.4509+1.4676x | 0.9768 | 2.36 | 1.85-2.31 | 17.53 | 10.01-31.21 | |
| Proportioning 1 | Y＝3.4511+1.4232x | 0.9849 | 12.15 | 10.04-14.95 | 97.46 | 59.53-159.55 | 1.32 |
| Proportioning 2 | Y＝3.3440+1.3958x | 0.9774 | 15.37 | 11.82-19.96 | 127.24 | 65.27-248.04 | 1.43 |
| Proportioning 3 | Y＝3.4250+1.1899x | 0.9978 | 20.92 | 19.56-22.70 | 251.57 | 204.70-309.18 | 1.31 |
| Proportioning 4 | Y＝3.3287+1.4097 | 0.9872 | 15.61 | 12.61-18.63 | 128.34 | 75.88-203.74 | 1.62 |
| Proportioning 5 | Y＝3.3182+1.1928x | 0.9412 | 25.39 | 17.04-38.77 | 305.07 | 94.12-988.82 | 1.14 |
By experimental result as can be known, Moroxydine Hydrochloride is to the EC of rice black-streaked dwarf virus
50Be 37.15mg/L (CL
0.95: 20.77-54.50), EC
50Be 388.88 (CL
0.95: 158.57-959.69), ribavirin is to the EC of black streaked dwarf virus of rice bacterium
50Be 2.39mg/L (CL
0.95: 1.87-2.30), EC
90Be 17.68 (CL
0.95: 10.06-31.07) zinc citrate is the part of polyphenol enzyme, the effect that when treatment virus, can make virus obtain effecting a permanent cure, be used for the sick control of paddy rice, show that Moroxydine Hydrochloride, ribavirin and zinc citrate cooperate the preventive effect to stripe disease significantly to be better than Moroxydine Hydrochloride.When Moroxydine Hydrochloride, ribavirin, zinc citrate are mixed by the mass ratio of 10: 1: 2,20: 1: 2,30: 1: 2,40: 1: 2 and 50: 1: 2, concentration in the inhibition of black streaked dwarf virus of rice is respectively 12.15mg/L, 15.37mg/L, 15.61mg/L, 20.92mg/L, 25.39mg/L, synergy ratio is respectively 1.32,1.43,1.62,1.31 and 1.14, all shows certain synergistic effect.Wherein, moroxydine, ribavirin and zinc citrate are 30: 1: 2 ratio when being mixed in active ingredient, synergic ratio the highest, and synergistic effect is best.
7, conclusion and discussion
According to Moroxydine Hydrochloride, ribavirin and zinc citrate to the formulation screening test result of black streaked dwarf virus of rice as can be known, Moroxydine Hydrochloride of the present invention, ribavirin, zinc citrate are composite effective be used to preventing and treating black streaked dwarf virus of rice, and the three is composite with Moroxydine Hydrochloride, ribavirin and the zinc citrate mass ratio preparation best results by 30: 1: 2, and synergistic effect is the most remarkable.
Claims (9)
1. Recompounded pesticide of preventing and treating black streaked dwarf virus of rice, it is characterized in that: the active ingredient of this Recompounded pesticide is Moroxydine Hydrochloride, ribavirin and zinc citrate, and described Recompounded pesticide comprises that mass fraction is 10-50 parts Moroxydine Hydrochloride, 0.5-1 part ribavirin and 1-2 parts zinc citrate.
2. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 1, it is characterized in that: described Recompounded pesticide comprises that mass fraction is 20-30 parts Moroxydine Hydrochloride, 0.5-1 part ribavirin and 1-2 parts zinc citrate.
3. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice of stating according to claim 2 is characterized in that: described Recompounded pesticide comprises that mass fraction is 30 parts Moroxydine Hydrochloride, 1 part ribavirin and 2 parts zinc citrate.
4. according to claim 1 to 3 each described Recompounded pesticides of preventing and treating black streaked dwarf virus of rice, it is characterized in that: described Recompounded pesticide is added with filler and the auxiliary agent of following mass fraction, and described auxiliary agent is that 0.5-1 part of kinase, 3-5 parts pull open powder, 3-5 parts of sodium lignin sulfonates and 5-10 parts of wetting agents; Described filler is 10-20 parts of potassium dihydrogen phosphates, 5-10 parts of sodium nitrate and 10-16 parts of bacillus licheniformis offcuts.
5. according to claim 1 to 3 each described Recompounded pesticides of preventing and treating black streaked dwarf virus of rice, it is characterized in that: described Recompounded pesticide is added with filler and the auxiliary agent of following mass fraction, and described auxiliary agent is that 1 part of kinase, 5 parts pull open powder, 5 parts of sodium lignin sulfonates and 10 parts of wetting agents; Described filler is 20 parts of potassium dihydrogen phosphates, 10 parts of sodium nitrate and 16 parts of bacillus licheniformis offcuts.
6. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 4, it is characterized in that: described kinase is coacetylase, described wetting agent is ammonium sulfate.
7. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 4, it is characterized in that: the preparation method of described bacillus licheniformis offcuts is that the filter residue of bacillus licheniformis after fermentation, washing, crystallization, filtration forms through drying, pulverizing.
8. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 5, it is characterized in that: described kinase is coacetylase, described wetting agent is ammonium sulfate.
9. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 5, it is characterized in that: the preparation method of described bacillus licheniformis offcuts is that the filter residue of bacillus licheniformis after fermentation, washing, crystallization, filtration forms through drying, pulverizing.
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| CN1647647A (en) * | 2004-11-30 | 2005-08-03 | 宣建荣 | Medicine for preventing and controlling plant virus and dead seedling and its preparing method |
| CN1935750A (en) * | 2005-09-20 | 2007-03-28 | 刘家宝 | Anti-plant-virus fertilizer additivesand fertilizer containing them |
| CN101317577A (en) * | 2008-07-02 | 2008-12-10 | 泰安市先禾农业科技研究所 | Medicament for preventing and controlling plant virus disease |
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| CN1647647A (en) * | 2004-11-30 | 2005-08-03 | 宣建荣 | Medicine for preventing and controlling plant virus and dead seedling and its preparing method |
| CN1935750A (en) * | 2005-09-20 | 2007-03-28 | 刘家宝 | Anti-plant-virus fertilizer additivesand fertilizer containing them |
| CN101317577A (en) * | 2008-07-02 | 2008-12-10 | 泰安市先禾农业科技研究所 | Medicament for preventing and controlling plant virus disease |
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