CN101779653A - Formulated pesticide for preventing black streaked dwarf of rice - Google Patents
Formulated pesticide for preventing black streaked dwarf of rice Download PDFInfo
- Publication number
- CN101779653A CN101779653A CN201010106528A CN201010106528A CN101779653A CN 101779653 A CN101779653 A CN 101779653A CN 201010106528 A CN201010106528 A CN 201010106528A CN 201010106528 A CN201010106528 A CN 201010106528A CN 101779653 A CN101779653 A CN 101779653A
- Authority
- CN
- China
- Prior art keywords
- rice
- streaked dwarf
- black streaked
- recompounded pesticide
- dwarf virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the field of pesticide technology and relates to a formulated pesticide for preventing black streaked dwarf of rice; the effective ingredients of the formulated pesticide are moroxydine hydrochloride, ribavirin and zinc citrate; the formulated pesticide for preventing black streaked dwarf of rice has the advantages that: the prevention and control effect is good with more than 90 percent; the product which is used scientifically and rationally is safe for rice, the water solubility is good, the quick acting effect is good, the formulated pesticide can prevent the black streaked dwarf which has resistance to the existing medicines, the formulated pesticide has no adverse effect to beneficial living beings and has no toxicity to people and livestock.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of Recompounded pesticide of preventing and treating black streaked dwarf virus of rice.
Background technology
In the real process of agricultural production, various disease conditions also can take place in plant, and black streaked dwarf virus of rice (Ricestipe virus is called for short RSV) is a kind of rice disease that is caused by virus, generation is all arranged, serious harm paddy rice quality and output in each paddy rice producing region, the whole nation.Because black streaked dwarf virus of rice is also thought the cancer of paddy rice by a lot of experts, according to the plant protection department statistics, black streaked dwarf virus of rice account for paddy rice due to illness the insect pest underproduction 43.63%.Black streaked dwarf virus of rice is a kind of novel very strong chronic disease of pesticide resistance, affects the output of crops for a long time, and this has just required corresponding agricultural chemicals to control black streaked dwarf virus of rice, improves actual control efficiency, thereby improves rice yield.
Summary of the invention
The object of the present invention is to provide a kind of Recompounded pesticide of preventing and treating black streaked dwarf virus of rice, control efficiency is good.
The present invention is by the following technical solutions:
A kind of Recompounded pesticide of preventing and treating black streaked dwarf virus of rice, the active ingredient of this Recompounded pesticide are Moroxydine Hydrochloride, ribavirin and zinc citrate.
Described Recompounded pesticide comprises that mass fraction is the Moroxydine Hydrochloride of 10-50 part, the ribavirin of 0.5-1 part and the zinc citrate of 1-2 part.
Described Recompounded pesticide comprises that mass fraction is the Moroxydine Hydrochloride of 20-30 part, the ribavirin of 0.5-1 part and the zinc citrate of 1-2 part.
Described Recompounded pesticide comprises that mass fraction is 30 parts Moroxydine Hydrochloride, 1 part ribavirin and 2 parts zinc citrate.
Described Recompounded pesticide is added with the filler and the auxiliary agent of following mass fraction, and described auxiliary agent is that 0.5-1 part kinase, 3-5 part are drawn back powder, 3-5 part sodium lignin sulfonate and 5-10 part wetting agent; Described filler is 10-20 part potassium dihydrogen phosphate, 5-10 part sodium nitrate and 10-16 part bacillus licheniformis offcuts.
Described Recompounded pesticide is added with the filler and the auxiliary agent of following mass fraction, and described auxiliary agent is that 1 part of kinase, 5 parts draw back powder, 5 parts of sodium lignin sulfonates and 10 parts of wetting agents; Described filler is 20 parts of potassium dihydrogen phosphates, 10 parts of sodium nitrate and 16 parts of bacillus licheniformis offcuts.
Described kinase is a coacetylase, and described wetting agent is an ammonium sulfate.
The preparation method of described bacillus licheniformis offcuts forms through drying, pulverizing for the filter residue of bacillus licheniformis after fermentation, washing, crystallization, filtration.
Preparation method of the present invention gets final product for mixing after each raw material pulverizing.
The using method of Recompounded pesticide of the present invention is converted water by Recompounded pesticide and is diluted to 800-1000 doubly, foliage-spray, and formulation rate is per hectare 450-750 gram Recompounded pesticide, the active ingredient dosage is a 148.5-247.5 gram/hectare.
The black streaked dwarf virus of rice main cause is a kind of virus disease that small brown rice planthopper or soil severe contamination cause, in case black-streaked dwarf virus take place then occur: Lao Ye blackout, embrittlement, harden, core leaf hai roll, shrinkage, not long, the bottom secondary root is undeveloped or do not have living secondary root, and the dead dead seedling of seedling is not eared; What The present invention be directed to the research and development of this disease has treatment, roots out the Recompounded pesticide with prevention effect, is the soluble powder of active ingredient with Moroxydine Hydrochloride, ribavirin and zinc citrate.Wherein, Moroxydine Hydrochloride mainly suppresses rice black-streaked dwarf virus albumen and generates, virus can not be invaded and duplicate, ribavirin mainly destroys the germ nucleosides and forms, and to the virus of having invaded it is solidified, and suppresses conidial formation on the scab, strong and the two-way conduction of energy of interior absorption, in the new blade that grows after the soup energy transporting dispenser that rice leaf absorbs, the medicament of rice root absorption can transporting arrive blade and cob part, thus the performance preventing disease theraping effect; Zinc citrate is that the part of polyphenol enzyme is (with zinc citrate and without zinc sulphate, the excessive easy generation poisoning zinc citrate of zinc sulphate then not can), potassium dihydrogen phosphate in the filler is the requisite nutrient components of crops, kinase in the auxiliary agent is that coacetylase is the constituent of primary juice, thereby particularly paddy rice is had take root, carry out photosynthetic, make chlorophyll, promote primary juice to flow, make the effect that it grows young leaves, the heading of loosing core can make virus obtain effecting a permanent cure when treatment virus, be used for thoroughly rooting out and preventing of black streaked dwarf virus of rice.
The present invention seeks a kind of medicament that can root out effectively, prevent and treat black streaked dwarf virus of rice, now that Moroxydine Hydrochloride, ribavirin and zinc citrate is composite, and filter out the best compound proportion of its synergistic effect, thereby effectively prevent and treat the generation of black streaked dwarf virus of rice; Contain a large amount of bacillus licheniformis active ingredient in the bacillus licheniformis offcuts among the present invention, belong to biological bactericide, can suppress the pathogen growth breeding.The Recompounded pesticide exploitation that the present invention prevents and treats black streaked dwarf virus of rice has solved a great problem with research for plant protection, by on paddy rice, using this product, its drug effect of verification and economic benefit provide scientific basis for the large scale application of this product, welcome by the rice farming, obtained remarkable economic efficiency and social benefit.
The Recompounded pesticide of preventing and treating black streaked dwarf virus of rice of the present invention has the following advantages:
(1) control efficiency is good, and preventive effect can reach 90.34%～95.41%; (2) this product of scientific and reasonable use is to rice safety; Seedling stage, transplanting date, heading stage, blooming stage all can use; (3) good water solubility, quick-acting is good, can prevent and treat the black streak dwarf that existing medicament is had resistance; (4) the field beneficial organism is not seen harmful effect, to people and animals' low toxicity.
Embodiment
Embodiment 1
The composition of Recompounded pesticide is: 50 parts Moroxydine Hydrochloride, 1 part ribavirin, 2 parts zinc citrate, 1 part of coacetylase, 5 parts draw back powder, 5 parts of sodium lignin sulfonates and 10 parts of ammonium sulfate, 20 parts of potassium dihydrogen phosphates, 10 parts of sodium nitrate and 16 parts of bacillus licheniformis offcuts.
Embodiment 2
The composition of Recompounded pesticide is: 10 parts Moroxydine Hydrochloride, 0.5 part ribavirin, 1 part zinc citrate, 0.5 part of coacetylase, 3 parts draw back powder, 3 parts of sodium lignin sulfonates and 5 parts of ammonium sulfate, 10 parts of potassium dihydrogen phosphates, 5 parts of sodium nitrate and 10 parts of bacillus licheniformis offcuts.
Embodiment 3
The composition of Recompounded pesticide is: 20 parts Moroxydine Hydrochloride, 0.8 part ribavirin, 1.5 parts zinc citrate, 0.8 part of coacetylase, 4 parts draw back powder, 4 parts of sodium lignin sulfonates and 8 parts of ammonium sulfate, 15 parts of potassium dihydrogen phosphates, 8 parts of sodium nitrate and 12 parts of bacillus licheniformis offcuts.
Embodiment 4
The composition of Recompounded pesticide is: 30 parts Moroxydine Hydrochloride, 1 part ribavirin, 2 parts zinc citrate, 1 part of coacetylase, 5 parts draw back powder, 5 parts of sodium lignin sulfonates and 10 parts of ammonium sulfate, 20 parts of potassium dihydrogen phosphates, 10 parts of sodium nitrate and 16 parts of bacillus licheniformis offcuts.
Umber among the above embodiment is mass fraction, and the preparation method of the bacillus licheniformis offcuts among the above embodiment forms through drying, pulverizing for the filter residue of bacillus licheniformis after fermentation, washing pili, water lotion crystallization, crystalline mixture filter.
Below prevent and treat the test portion of the Recompounded pesticide of black streaked dwarf virus of rice for the present invention:
With embodiment 4 Recompounded pesticides is the field experiment report of example:
1, demonstration test
1.1 demonstration place and basic condition: demonstration test is chosen in Sang Cun administrative village, Hua Xiansangcun township, Henan Province demonstration paddy field.Soil is two to close class soil, and organic 1.58%, PH7.1 is the higher Rice Production plot of fertility.
1.2 rice varieties and planting type: for showing that (examination) paddy rice is " No. 1, ruddiness " each field, sub-district piece conventional cultivation management.
1.3 the method to set up of demonstration test: demonstration is chosen in 50 mu of paddy rice plot in flakes of equal cultivation management condition, in the black streaked dwarf virus of rice their early stage, even sprayed 2 times every 10 days,, handle I: add 30g embodiment 4 Recompounded pesticides by every mu of 30kg clear water by every mu of 30kg clear water; Handle II: add 40g embodiment 4 Recompounded pesticides by every mu of 30kg clear water; Handle III: add 50g embodiment 4 Recompounded pesticides by every mu of 30kg clear water; The foliage-spray with each sub-district of time is carried out in three kinds of processing, and carries out the paired observation of demonstration test.
2, demonstration test result and analysis
2.1 result of the test as can be seen, along with concentration increases, embodiment 4 Recompounded pesticides obviously increase the preventive effect of black streaked dwarf virus of rice, see the following form 1.
Table 1 embodiment 4 Recompounded pesticides are prevented and treated the black streaked dwarf virus of rice result
| Handle medicament | Effective ingredient consumption (gram/hectare) | Preventive effect after the last dispenser (%) (respectively repeating mean value) |
| Handle I | ??148.5 | ??80.5 |
| Handle II | ??198 | ??86.9 |
| Handle III | ??247.5 | ??92.3 |
| Clear water | ??/ | ??/ |
2.2 show that by experimental observation embodiment 4 Recompounded pesticides are to rice safety, no side effects takes place.
3, conclusion
" No. 1, ruddiness " is on the identical basis of field management measure, foliage-spray embodiment 4 Recompounded pesticides begin dispenser in the first period of disease of paddy rice black streaked dwarf virus of rice, spray 1 time after 10 days again paddy rice black streaked dwarf virus of rice disease had good control efficiency, adopt embodiment 4 Recompounded pesticide 148.5-247.5 gram (per hectare effective ingredient consumption) continuous administration 2 times, average preventive effect can reach 92.3% effect after 10 days, and to rice safety.
4, overall merit
Show through the demonstration test operating position: (1) control efficiency is good, and preventive effect can reach 95%; (2) this product of scientific and reasonable use is to rice safety; (3) water soluble quick-acting is good, can prevent and treat the black streaked dwarf virus of rice that existing medicament is had resistance; (4) the field beneficial organism is not seen harmful effect, to people and animals' low toxicity.
The ecotoxicology data of embodiment 4 Recompounded pesticides
1 experiment material
1.1 reagent agent
Embodiment 4 Recompounded pesticides
1.2 it is biological for examination
1.2.1 for the examination honeybee
Apis mellifera (Apis mellifera L.), the worker bee that grows up, animal science institute of Zhejiang University provides.
1.2.2 for the examination birds
Japanese quail (Coturnix coturnix japonica), city proper and areas just outside its gates town, Xiaoshan, Hangzhou fowl and livestock farm provides.Selecting the length of time for use is 30 days, healthy active quail confession examination.Before the test under the laboratory test condition one week of precuring, during the precuring quail all growth is normal, no illness does not have death.
1.2.3 for the examination fish
The test fingerling is zebra fish (rachydanio rerio), average body long (connecting tail) 3.0cm, the about 0.180g of average weight.Provide by the aquarium of setting sail, Zhejiang.Raise and train a week indoor before the test, the zebra fish growth does not normally have death during raising and train, and no illness is all healthy, big or small basically identical.Test stops feeding the previous day, and duration of test is feeding not.
1.2.4 for the examination silkworm
For the examination silkworm egg is autumn wind * white jade, plays silkworm two ages for examination, and silkworm egg is provided by Zhejiang Province's silkworm egg company.
2 experimental instrument and equipments
2.1 test peak cage: 500ml glass beaker (sealing) with gauze;
2.2 test birdcage: plastic-coated iron cage, long 50cm, wide 35cm, high 35cm, two of band feed box are given one of water tumbler;
2.3 test fish jar: clear glass cylinder, internal diameter 30cm, high 30cm;
2.4 other: 1ml syringe, 16# irritate stomach pin, volumetric flask, beaker, thermometer, pipette, liquid-transfering gun, 5ml small beaker, absorbent cotton, pH meter, dissolved oxygen meter, artificial climate incubator etc.
3 test methods
3.1 honeybee test
Adopt the absorption method to investigate the absorption toxicity of medicament.
Refined sugar solution with 50% is mixed with embodiment 4 Recompounded pesticides the soup of a plurality of variable concentrations for examination.Get the soup 5ml for preparing and move into and be equipped with in the 5ml small beaker of absorbent cotton, again small beaker is put in the 500ml large beaker honeyflow honeybee and gets food.
In large beaker, move into 20 adult workers bee, seal with gauze.Each handles triplicate, establishes blank.Test is carried out under poor light condition, 25 ± 1 ℃ of environmental temperatures, and relative moisture 70 ± 5% is after the chemicals treatment 24, the 48h investigation situation of being poisoned to death.
3.2 birds test
25 ± 2 ℃ of test temperatures, the room ventilation printing opacity.Feed adopts the quail feed, feeds with clear water simultaneously.Test quail 10 every group (male and female half and half) is as a repetition.Preceding fasting 12-20 hour of test is only for clear water.Embodiment 4 Recompounded pesticides with distilled water diluting after for the examination, determine the toxicity test scope by prerun.Formal test is provided with some groups of variable concentrations soups, and other establishes one group and irritates clear water as blank, and 1 repetition is established in each processing.The per os contamination administration by gavage that test adopts disposable dosage directly to inject.With the soup of variable concentrations by the processing of contaminating of the dosage of every 100g body weight quail perfusion 1ml.Quail after the processing is raised according to a conventional method, poisoning symptom of observed and recorded quail and death figure.Test lasts 7 days, and the death figure accumulation is calculated.Calculate the medial lethal dose LD of medicament with the DPS data processing platform (DPP) to quail
50Value and 95% confidence limit CL
0.95.
3.3 pilot fish
Adopt static method.
Laboratory temperature is controlled at 22 ± 1 ℃. and test water deposits and aeration, test are raised and train and tested all with fish and carry out in the laboratory.Test water is a running water, and more than 24 hours, hardness 86mg/L is (with CaCO through the gas disposal of aeration dechlorination
3Meter).Test chamber is positioned in the Water Tank with Temp.-controlled of continuous-flow type device, 22 ± 1 ℃ of water temperatures. the confession reagent liquid of 10 liters of variable concentrations of preparation in each test cylinder (jar), and drop into 10 of zebra fishs.Determine the toxicity test concentration range by preliminary experiment.Formal test is provided with the soup of some groups of variable concentrations, and other establishes one group of blank, and 3 repetitions are established in each processing.Regularly measure the dissolved oxygen and the pH value of test solution in each cylinder in the experiment.Test lasts 96 hours, continuously observed and recorded 24,48,72,96h zebra fish poisoning symptom and death figure, in time remove dead fish.Test is handled and is adopted the DPS data processing platform (DPP) to calculate the half lethal concentration (LC of medicament to zebra fish
50) value.
3.4 silkworm test
Embodiment 4 Recompounded pesticides are mixed with the soup of several variable concentrations with deionized water.With the soup dipping 10g mulberry leaf of variable concentrations, dip time is 10 seconds, treats to supply behind the soup natural air drying on the mulberry leaf to nibble usefulness, moves in every diameter 15cm culture dish and plays silkworm, triplicate 20 two ages.If blank.
Test is carried out under poor light condition, 25 ± 1 ℃ of temperature, and relative moisture 70 ± 5%, processing back 24,48,96h to three rise age, are tried poisoning symptom and the death condition of silkworm.Experiment finishes the back data is carried out data statistics.
4 experimental results and toxicity assessment
4.1 honeybee test
The contrast honeybee is movable and get food normally.The calomel poisoning honeybee is relatively more excited, constantly creeps, and easily falls on the clump wall of cup; Serious poisoning honeybee is more weak, or the struggle of rollover back, and the digitus and the feeler that have are slowly brandished, and what have constantly smoothes out with the fingers rafter with front foot.Dead honeybee wing opens, and the leg purse up colludes Fu, Young-locust-without-wings and stretches out.The lethality of test liquor strength and each group honeybee sees Table 2, and statistics sees Table 3.
Table 2 embodiment 4 Recompounded pesticides are to the absorption toxicity test result of honeybee
Table 3 embodiment 4 Recompounded pesticides are to the lethal concentration of 50 of honeybee
According to " chemical pesticide environmental safety assessment test rule " to the honeybee toxic grade criteria for classifying; LC
50Value≤0.mg/L's is highly toxic pesticide; 0.5mg/L≤LC
50＜20mg/L's is the height agricultural chemicals; 20mg/L＜LC
50≤ 200mg/L's is medium toxic pesticides; LC
50＞00mg/L is a low-toxin farm chemicals.Experimental result shows that embodiment 4 Recompounded pesticides are to the absorption toxicity LC of honeybee
50Being 3.4053mg/L when 48h, is high poison to honeybee toxicity.
4.2 birds test
The quail growth does not normally have dead the generation in the contrast of experimentation empty.
In 8 hours, tried quail and do not had obvious poisoning symptom after the administration, do not had dead the generation; Administration is observed after handling 24h, does not have newly-increased death, and quail is movable normal; No new death takes place in the follow-up observation period.Test liquor strength and experimental result see Table 4, and statistics sees Table 5.
Table 4 embodiment 4 Recompounded pesticides are to the absorption toxicity test result of quail
Table 5 embodiment 4 Recompounded pesticides are to the lethal dose of 50 (LD of quail
50)
According to " chemical pesticide environmental safety assessment test rule " the toxic grade criteria for classifying to bird: LD
50≤ 10mg/kg is a highly toxic pesticide; 10mg/kg＜LD
50≤ 500mg/kg is a medium toxic pesticides; LD
50＞500mg/kg is a low-toxin farm chemicals.This test shows that embodiment 4 Recompounded pesticides are low toxicity to the toxicity of birds.
4.3 pilot fish
Behind the contact soup, there is not poisoning symptom substantially.The lethality of test liquor strength and each group zebra fish sees Table 6, and statistics sees Table 7.
Table 6 embodiment 4 Recompounded pesticides are to the acute toxicity testing result of zebra fish
Table 7 embodiment 4 Recompounded pesticides are to the lethal concentration of 50 (LD of zebra fish
50)
According to " chemical pesticide environmental safety assessment test rule " to the toxicity in fish grading standard; LC
50≤ 0.1mg/L's is highly toxic pesticide; 0.1mg/L＜LC
50≤ 1.0mg/L's is high-toxic pesticide; 1.0mg/L＜LC
50≤ 10.0mg/L's is medium toxic pesticides; LC
50＞10.0mg/L is a low-toxin farm chemicals.Experimental result shows that embodiment 4 Recompounded pesticides are to 96 hours LC of zebra fish
50＞1000mg/L is so the toxicity of embodiment 4 Recompounded pesticides belongs to low toxicity.
4.4 silkworm test
Test different time observed result shows that the contrast silkworm is normally got food or creeps, and experiment beginning back 96h is all casted off a skin and become for three ages.24h does not have difference substantially with contrast; The 48h calomel poisoning, it is little to contrast the silkworm body; The 96h calomel poisoning, the silkworm individuality is less, and the silkworm body of seriously poisoning is stood transfixed to the ground, and has body fluid to ooze out on every side, and dead silkworm body shortens, blackout.Test liquor strength and experimental result see Table 8, and statistics sees Table 9.
Table 8 embodiment 4 Recompounded pesticides are to the acute toxicity testing result of silkworm
Table 9 embodiment 4 Recompounded pesticides are to the lethal concentration of 50 (LD of zebra fish
50)
According to " chemical pesticide environmental safety assessment test rule " to the silkworm class toxic grade criteria for classifying; LC
50≤ 0.5mg/L's is highly toxic pesticide; 0.5mg/L＜LC
50≤ 20mg/L's is high-toxic pesticide; 20mg/L＜LC
50≤ 200mg/L's is medium toxic pesticides; LC
50＞200mg/L is a low-toxin farm chemicals.Embodiment 4 Recompounded pesticides LC during to the 96h of silkworm
50＞982mg/L belongs to low toxicity to the toxicity of silkworm.
More than test the toxicity test that embodiment 4 Recompounded pesticides has been carried out four kinds of environmental organisms such as honeybee, quail, fish silkworm, for this agriculture chemical registration provides the ecotoxicology data.Measurement result is as follows:
Honeybee LC
50(48h) 3.4053mg/L
Quail LC
50(7d)＞1000mg/Kg
Zebra fish LC
50(96d)＞1000mg/L
Silkworm LC
50(two ages, 96d)＞982mg/L
According to toxicity and the risk grade scale of agricultural chemicals to environmental organism, embodiment 4 Recompounded pesticides are high poison to honeybee; To bird is low toxicity; To fish is low toxicity; To silkworm is low toxicity.
Embodiment 1 Recompounded pesticide field experiment report:
Field trial draws in the Wuzhi County, Henan, prevent and treat black streaked dwarf virus of rice with embodiment 1 Recompounded pesticide and have good control efficiency, 37-50 gram/even spraying per mu, medicine is the black streak dwarf rate 92.35% that goes down one day after, three days black streak dwarf rate 95.29% that goes down behind the medicine, seven days black streak dwarf rate 93.33% that goes down behind the medicine.
The field experiment report of embodiment 3 Recompounded pesticides:
Also carried out large tracts of land in Zhejiang and promoted demonstration test, test this time divides three processing, and spraying medicine concentration is respectively 35 gram/mus, 42.5 gram/mus, 50 gram/mus, and wherein 10 days black streak dwarf go down the rate average out to more than 90% behind the 35 gram/mu dispenser rice field medicines.
Meanwhile, also carry out large tracts of land in Jining, Shandong and promoted demonstration test, test this time divides three processing, spraying medicine concentration is respectively 35 gram/mus, 42.5 gram/mus, 50 gram/mus, wherein 35 gram/mu dispenser rice field medicines one day after the black streak dwarf rate of going down reach 92.54%, three days black streak dwarf rate 96.38% that goes down behind the medicine, seven days banded sclerotial blights rate 92.66% that goes down behind the medicine.
The formulation screening test of Recompounded pesticide of the present invention
Black streaked dwarf virus of rice is a kind of rice disease that has virus to cause, generation is all arranged, serious harm rice yield and quality in each rice belt, the whole nation.Now that Moroxydine Hydrochloride, ribavirin and zinc citrate is composite, and filter out its control, the best compound proportion of synergistic effect.
One, materials and methods
1, reagent agent
The former medicine of ribavirin and Moroxydine Hydrochloride and zinc citrate is commercially available, and its different proportioning mixtures are prepared voluntarily by the applicant.
2, for the examination material
For trying the water rice varieties, be with malicious small brown rice planthopper available from insect teaching and research room of Agricultural University Of He'nan for the susceptible force of black-streaked dwarf is educated japonica rice No. 3.
3, experiment equipment
Electronic balance (sensibility reciprocal 0.1mg), beaker, volumetric flask, glass rod, pipette, polypots, gauze.
4, test method
4.1 medicament preparation
With sterile water Moroxydine Hydrochloride is made into the 1000mg/L mother liquor, and dilutes 100,50,25,12.5 and 6.25 times, be mixed with concentration and be 10,20,40,80 and 160mg/L Moroxydine Hydrochloride series mass gradient solution for examination.
Adopting the compound concentration that uses the same method is the mother liquor of 100mg/L ribavirin, and with sterile water be mixed with concentration be 0.5,1,2,4 and 8mg/L ribavirin series mass gradient solution for the examination.
Adopting the compound concentration that uses the same method is the mother liquor of 100mg/L zinc citrate, and with sterile water be mixed with concentration be 1,2,3,4 and 8mg/L zinc citrate series mass gradient solution for the examination.
Respectively get an amount of volume mother liquor, is that the ratio of 10: 1: 2 (proportioning 1), 20: 1: 2 (proportioning 2), 30: 1: 2 (proportioning 3), 40: 1: 2 (proportioning 4) and 50: 1: 2 (proportioning 5) is mixed with Moroxydine Hydrochloride and ribavirin and zinc citrate in mass ratio, and, each assembly is supplied examination than the quality gradient solution that is diluted to variable concentrations with sterile water according to pre-test result:
Proportioning 1 moroxydine: ribavirin: zinc citrate=10: 1: 2 2.5,5,10,20,40mg/L
Proportioning 2 moroxydines: ribavirin: zinc citrate=20: 1: 2 2.5,5,10,20,40mg/L
Proportioning 3 moroxydines: ribavirin: zinc citrate=30: 1: 2 2.5,5,10,20,40mg/L
Proportioning 4 moroxydines: ribavirin: zinc citrate=40: 1: 25,10,20,40,80mg/L
Proportioning 5 moroxydines: ribavirin: zinc citrate=50: 1: 25,10,20,40,80mg/L;
4.2 test method
Adopt pot-culture method.Rice paddy seed is with the screening of 20%NaCl aqueous solution soaking, use flushing with clean water 5 times again after, seed soaking is 2 days under the room temperature, the vernalization 1 day of preserving moisture in 35 ℃ of incubators that drains away the water treats to be seeded in after seed shows money or valuables one carries unintentionally in the polypots that diameter is about 15cm 25 of every alms bowl sowings.Just grown to 2 leaves to the paddy rice seedling and rejected weak seedling during the phase, every alms bowl keeps the rice seedling of 15 strain growing way homogeneous, when most of rice seedling grows to 3 leaves, 1 heart, with being with malicious plant hopper inoculation rice black-streaked dwarf virus.24h carries out even spraying with the medicament for preparing to the rice leaf positive and negative and handles before the inoculation, with the fully moistening but degree of being that do not drip in blade face.Every processing repeats 3 times, and 1 reprocessing 15 strain is 1 pot culture.Medicament sprays and finishes, and every alms bowl covers translucent cover respectively, and seal with gauze on the top, by the quantity of 5 of every young plants, inserts 2-3 and is with malicious small brown rice planthopper age, every 6h left and right sides disturbance gauze once, makes tested seedling evenly be subjected to poison.Behind the 48h, small brown rice planthopper is all removed.After inoculating for 3 weeks, the control treatment state of an illness tends towards stability, and reinvestigates the black streaked dwarf virus of rice incidence.
5, investigation and statistical method
Investigation morbidity strain number and classification are write down all and are handled paddy rice state of an illness a situation arises, calculate disease index and suppress effect, adopt the DPS data handling system to calculate the EC of each medicament
50And EC
90And 95% confidence limit.
Black streaked dwarf virus of rice state of an illness grade scale is as follows:
5 grades: growing way is very poor, state of an illness full wafer or part blackout, obfuscation, embrittlement, hardens, new leaf rolling, the withered core of shrinkage, withered, do not ear; Root does not have secondary root.
4 grades: growing way is very poor, and the obfuscation of sick leaf is green but black not hard, the dark yellow leaf disease spot of bottom leaf continuously, at random, upper leaf has dark green symptom, black an IOU issued by a post office shape spot is big and many; The root secondary root is undeveloped.
3 grades: growing way is very poor, but sick leaf is black dark slightly, the dark green scab of lower part continuously, at random, spot enlarges, upper leaf has dark yellow symptom, the jaundice of core leaf is wrinkle slightly; The root secondary root is less.
2 grades: growing way a little less than, sick leaf is slightly dark, core leaf roll song, sick portion is slightly dark yellow, is fragmentary point-like or black dull strip, sick strong intersection is obviously clear, the root growth Non Apparent Abnormality;
1 grade: growing way slightly a little less than, fragmentary point-like is dark green, sick strong portion has at interval, sick leaf is dark slightly along vein, spot seldom, root growth is normal;
0 grade: no scab, growing way is fine, and the initial stage, visible very little scab was covered up with the growth of seedling.
Computing formula:
According to the coefficient of synergism SR of Wadely method calculating mixture, with the synergistic effect of the different proportionings of synergy ratio (SR) judgement, SR＜0.5 is an antagonism, and SR=0.5-1.5 is a summation action, and SR＞1.5 are synergistic effect.
The correlation computations formula is as follows:
A, B are the compositions of each bactericide in the formula, and a, b are the ratios of these compositions in mixture.
6, result and analysis
After 3 weeks, investigation Moroxydine Hydrochloride, ribavirin, zinc citrate different proportion Mixed Pharmacy are handled the incidence of back black streaked dwarf virus of rice, and experimental result is as shown in table 10:
The incidence of black streaked dwarf virus of rice after the different chemicals treatment of table 10
Utilize the DPSa data handling system to calculate the EC of each medicament
50, EC
90With 95% confidence interval (CL
0.95), and calculate the synergy ratio (SR) of five assembly ratio, experimental result such as table 11 by Wadely:
Table 11 Moroxydine Hydrochloride and ribavirin and zinc citrate are to the formulation screening test result of black streaked dwarf virus of rice bacterium
| Medicament | Regression equation | Correlation coefficient | ??EC50 | ??CL0.95 | ??EC90 | ??CL0.95 | ??SR |
| Moroxydine Hydrochloride | ??Y＝2.9883+1.2716x | ??0.9524 | ??37.15 | ??20.77-54.50 | ??388.88 | ??158.57-959.69 | |
| Ribavirin | ??Y＝4.4504+1.4679x | ??0.9789 | ??2.39 | ??1.87-2.30 | ??17.68 | ??10.06-31.07 | |
| Zinc citrate | ??Y＝4.4509+1.4676x | ??0.9768 | ??2.36 | ??1.85-2.31 | ??17.53 | ??10.01-31.21 | |
| Proportioning 1 | ??Y＝3.4511+1.4232x | ??0.9849 | ??12.15 | ??10.04-14.95 | ??97.46 | ??59.53-159.55 | ??1.32 |
| Proportioning 2 | ??Y＝3.3440+1.3958x | ??0.9774 | ??15.37 | ??11.82-19.96 | ??127.24 | ??65.27-248.04 | ??1.43 |
| Proportioning 3 | ??Y＝3.4250+1.1899x | ??0.9978 | ??20.92 | ??19.56-22.70 | ??251.57 | ??204.70-309.18 | ??1.31 |
| Proportioning 4 | ??Y＝3.3287+1.4097 | ??0.9872 | ??15.61 | ??12.61-18.63 | ??128.34 | ??75.88-203.74 | ??1.62 |
| Proportioning 5 | ??Y＝3.3182+1.1928x | ??0.9412 | ??25.39 | ??17.04-38.77 | ??305.07 | ??94.12-988.82 | ??1.14 |
By experimental result as can be known, Moroxydine Hydrochloride is to the EC of rice black-streaked dwarf virus
50Be 37.15mg/L (CL
0.95: 20.77-54.50), EC
50Be 388.88 (CL
0.95: 158.57-959.69), ribavirin is to the EC of black streaked dwarf virus of rice bacterium
50Be 2.39mg/L (CL
0.95: 1.87-2.30), EC
90Be 17.68 (CL
0.95: 10.06-31.07) zinc citrate is the part of polyphenol enzyme, the effect that when treatment virus, can make virus obtain effecting a permanent cure, be used for the sick control of paddy rice, show that Moroxydine Hydrochloride, ribavirin and zinc citrate cooperate the preventive effect to stripe disease significantly to be better than Moroxydine Hydrochloride.When Moroxydine Hydrochloride, ribavirin, zinc citrate are mixed by the mass ratio of 10: 1: 2,20: 1: 2,30: 1: 2,40: 1: 2 and 50: 1: 2, concentration in the inhibition of black streaked dwarf virus of rice is respectively 12.15mg/L, 15.37mg/L, 15.61mg/L, 20.92mg/L, 25.39mg/L, synergy ratio is respectively 1.32,1.43,1.62,1.31 and 1.14, all shows certain synergistic effect.Wherein, moroxydine, ribavirin and zinc citrate are 30: 1: 2 ratio when being mixed in active ingredient, synergic ratio the highest, and synergistic effect is best.
7, conclusion and discussion
According to Moroxydine Hydrochloride, ribavirin and zinc citrate as can be known to the formulation screening test result of black streaked dwarf virus of rice, Moroxydine Hydrochloride of the present invention, ribavirin, zinc citrate be composite, and to be used to prevent and treat black streaked dwarf virus of rice effective, and the three is composite with Moroxydine Hydrochloride, ribavirin and the zinc citrate mass ratio preparation best results by 30: 1: 2, and synergistic effect is the most remarkable.
Claims (9)
1. Recompounded pesticide of preventing and treating black streaked dwarf virus of rice, it is characterized in that: the active ingredient of this Recompounded pesticide is Moroxydine Hydrochloride, ribavirin and zinc citrate.
2. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 1 is characterized in that: described Recompounded pesticide comprises that mass fraction is the Moroxydine Hydrochloride of 10-50 part, the ribavirin of 0.5-1 part and the zinc citrate of 1-2 part.
3. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 2 is characterized in that: described Recompounded pesticide comprises that mass fraction is the Moroxydine Hydrochloride of 20-30 part, the ribavirin of 0.5-1 part and the zinc citrate of 1-2 part.
4. the Recompounded pesticide of stating according to claim 3 of preventing and treating black streaked dwarf virus of rice, it is characterized in that: described Recompounded pesticide comprises that mass fraction is 30 parts Moroxydine Hydrochloride, 1 part ribavirin and 2 parts zinc citrate.
5. according to each described Recompounded pesticide of preventing and treating black streaked dwarf virus of rice of claim 2 to 4, it is characterized in that: described Recompounded pesticide is added with the filler and the auxiliary agent of following mass fraction, and described auxiliary agent is that 0.5-1 part kinase, 3-5 part are drawn back powder, 3-5 part sodium lignin sulfonate and 5-10 part wetting agent; Described filler is 10-20 part potassium dihydrogen phosphate, 5-10 part sodium nitrate and 10-16 part bacillus licheniformis offcuts.
6. according to each described Recompounded pesticide of preventing and treating black streaked dwarf virus of rice of claim 2 to 4, it is characterized in that: described Recompounded pesticide is added with the filler and the auxiliary agent of following mass fraction, and described auxiliary agent is that 1 part of kinase, 5 parts draw back powder, 5 parts of sodium lignin sulfonates and 10 parts of wetting agents; Described filler is 20 parts of potassium dihydrogen phosphates, 10 parts of sodium nitrate and 16 parts of bacillus licheniformis offcuts.
7. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 5 is characterized in that: described kinase is a coacetylase, and described wetting agent is an ammonium sulfate.
8. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 5 is characterized in that: the preparation method of described bacillus licheniformis offcuts forms through drying, pulverizing for the filter residue of bacillus licheniformis after fermentation, washing, crystallization, filtration.
9 Recompounded pesticides of preventing and treating black streaked dwarf virus of rice according to claim 6 is characterized in that: described kinase is a coacetylase, and described wetting agent is an ammonium sulfate.
10. the Recompounded pesticide of preventing and treating black streaked dwarf virus of rice according to claim 6 is characterized in that: the preparation method of described bacillus licheniformis offcuts forms through drying, pulverizing for the filter residue of bacillus licheniformis after fermentation, washing, crystallization, filtration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010106528 CN101779653B (en) | 2010-02-05 | 2010-02-05 | Formulated pesticide for preventing black streaked dwarf of rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010106528 CN101779653B (en) | 2010-02-05 | 2010-02-05 | Formulated pesticide for preventing black streaked dwarf of rice |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101779653A true CN101779653A (en) | 2010-07-21 |
| CN101779653B CN101779653B (en) | 2013-04-10 |
Family
ID=42519906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010106528 Active CN101779653B (en) | 2010-02-05 | 2010-02-05 | Formulated pesticide for preventing black streaked dwarf of rice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101779653B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102515971A (en) * | 2011-12-11 | 2012-06-27 | 湖北仙隆化工股份有限公司 | Rice seed dressing agent composition used for preventing and treating black-streaked dwarf disease and its preparation method |
| CN104996451A (en) * | 2015-08-06 | 2015-10-28 | 陆月霞 | Bactericide for treating northern leaf blight of corn |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1647647A (en) * | 2004-11-30 | 2005-08-03 | 宣建荣 | Medicine for preventing and controlling plant virus and dead seedling and its preparing method |
| CN1935750A (en) * | 2005-09-20 | 2007-03-28 | 刘家宝 | Anti-plant-virus fertilizer additivesand fertilizer containing them |
| CN101317577A (en) * | 2008-07-02 | 2008-12-10 | 泰安市先禾农业科技研究所 | Medicament for preventing and controlling plant virus disease |
-
2010
- 2010-02-05 CN CN 201010106528 patent/CN101779653B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1647647A (en) * | 2004-11-30 | 2005-08-03 | 宣建荣 | Medicine for preventing and controlling plant virus and dead seedling and its preparing method |
| CN1935750A (en) * | 2005-09-20 | 2007-03-28 | 刘家宝 | Anti-plant-virus fertilizer additivesand fertilizer containing them |
| CN101317577A (en) * | 2008-07-02 | 2008-12-10 | 泰安市先禾农业科技研究所 | Medicament for preventing and controlling plant virus disease |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102515971A (en) * | 2011-12-11 | 2012-06-27 | 湖北仙隆化工股份有限公司 | Rice seed dressing agent composition used for preventing and treating black-streaked dwarf disease and its preparation method |
| CN102515971B (en) * | 2011-12-11 | 2013-09-04 | 湖北仙隆化工股份有限公司 | Rice seed dressing agent composition used for preventing and treating black-streaked dwarf disease and its preparation method |
| CN104996451A (en) * | 2015-08-06 | 2015-10-28 | 陆月霞 | Bactericide for treating northern leaf blight of corn |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101779653B (en) | 2013-04-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102674992A (en) | Special active biological medicine fertilizer for vegetables | |
| CN102318638A (en) | Corn seed coating agent | |
| CN105284864A (en) | Forest fruit trunk-injection insecticide and preparation method and application thereof | |
| CN102633570A (en) | Special active biological medicine fertilizer for hot pepper | |
| CN102674993A (en) | Active biological pesticide fertilizer special for rape plant | |
| CN102204564B (en) | Pesticide composition containing fosthiazate and carbosulfan | |
| CN104839152A (en) | Environment-friendly type plant protective agent and preparation method thereof | |
| CN102643143A (en) | Active biological medicine fertilizer specially used for walnut tree | |
| CN104817406A (en) | Plant protection composition containing chitosan oligosaccharide and dinotefuran | |
| CN109362740A (en) | Contain Rynaxypyr and fluopyram, the seed coat agent of gamma cyhalothrin and purposes | |
| CN103880563B (en) | A kind of cultivation matrix and preparation method thereof and application | |
| CN102484994B (en) | Synergy composition of matrine and chlorantraniliprole | |
| CN101779653B (en) | Formulated pesticide for preventing black streaked dwarf of rice | |
| CN104705349B (en) | The preparation at white lotus parthenium extract different activities position and its application as acaricidal activity material | |
| CN103355322A (en) | Compound biological insecticide and application thereof | |
| CN101396024B (en) | Dendrolimus punctatus cytoplasmic polyhedrosis virus(dpcpv) bacillus thuringiensis wettable powder and preparation method thereof | |
| CN104817409B (en) | A kind of Sugarcane Fertilization method | |
| CN103478129A (en) | Agricultural compound bactericide and application thereof | |
| KR102627671B1 (en) | Composition of high concentration saponin fertilizer including ellow lacquer extract, The fertilizer using the composition, And the cultivation grow using the fertilizer | |
| CN105685110A (en) | Plant source composite for preventing cutworms and plant source insecticide and application thereof | |
| CN106376598B (en) | Pesticidal combination containing Empedobacter brevis and Cupric sulfate | |
| Khatun et al. | Insecticidal activity of Acacia catechu bark extract against four stored product pests | |
| CN105941423B (en) | A kind of Pesticidal combination containing capillary and cyromazine | |
| CN110384103A (en) | Contain benziothiazolinone and fluopyram, the seed coat agent of Rynaxypyr and purposes | |
| CN110896773A (en) | Pollution-free prevention and control method for diseases and pests of apple trees |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |