CN104782639A - Novel method for preventing and controlling nilaparvata lugens filial generation by using fungicide - Google Patents
Novel method for preventing and controlling nilaparvata lugens filial generation by using fungicide Download PDFInfo
- Publication number
- CN104782639A CN104782639A CN201510193541.5A CN201510193541A CN104782639A CN 104782639 A CN104782639 A CN 104782639A CN 201510193541 A CN201510193541 A CN 201510193541A CN 104782639 A CN104782639 A CN 104782639A
- Authority
- CN
- China
- Prior art keywords
- tetramycin
- tetrin
- brown planthopper
- fungicide
- toyokamycin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of biological control, in particular to a novel method for preventing and controlling nilaparvata lugens filial generation by using fungicide. According to the novel method, 27% of toyocamycin & tetramycin P & tetrin B & tetramycin A (which containing 5.58% of toyocamycin, 7.09% of tetramycin P, 4.44% of tetrin B and 9.64% of tetramycin A by mass percentage) is used for preventing and controlling the nilaparvata lugens filial generation. The novel method has the most notable feature that the toyocamycin & tetramycin P & tetrin B & tetramycin A prevent or inhibit that yeast-like symbiotes enter from hemolymph of nilaparvata lugens female adults to ovarian tissues, so that the reproductive system of nilaparvata lugens is affected, the spawning amount is reduced, the incubation rate of the spawned eggs is also reduced due to the lack of symbiotic bacteria, and the second generation of nilaparvata lugens is controlled effectively.
Description
Technical field
The present invention relates to technical field of biological control, particularly relate to a kind of new method utilizing fungicide to prevent and treat brown planthopper filial generation.
Background technology
Brown planthopper is the important rice grub of Asia, and the primary pest in Ye Shi China Yangtze river basin, south China and southwestern rice district, causes high risks to China's Rice Production.Existing prevention and controls is mainly through promoting insect-proof rice kind or controlling brown planthopper by spraying insecticide.Insect-proof rice kind has certain control action to Population of Rice Brown Planthopper in a short time, but brown planthopper has very strong adaptability to pest-resistant rice varieties, and 3 generations of general breeding are energy adapted breed resistance just, thus needs to be aided with insecticide to prevent and treat brown planthopper.But, because a large amount of of chemical insecticide frequently make brown planthopper to which creating pesticide resistance, cause control efficiency obviously to decline.
There is a large amount of fungal component in brown planthopper fat-body, wherein, Yeast-like symbionts plays a part indispensable to growing of brown planthopper, and lacking the brown planthopper of Yeast-like symbionts can not normal development or survival.After brown planthopper sprouts wings, Yeast-like symbionts passes through brown planthopper ovary with ovum vertical transmission to filial generation brown planthopper.In vertical transmission process, Yeast-like symbionts dissociates to hemolymph from the fat-body of brown planthopper female adult pest, then enters ovary tissue, enters the egg mother cell of brown planthopper finally by endocytosis, thus completes the communication process from female generation to filial generation.In above-mentioned communication process, dissociate to the Yeast-like symbionts hemolymph from the fat-body of brown planthopper female adult pest, than the impact being subject to fungicide and other external factors easier in fat-body.
Since Yeast-like symbionts vertical transmission between brown planthopper generation, and Yeast-like symbionts and brown planthopper growth and breeding relation very tight, therefore, occur and reduce brown planthopper to cause harm to control filial generation brown planthopper by the means of " suppress or stop Yeast-like symbionts to enter the ovary of brown planthopper; the ovum making brown planthopper give birth to lacks Yeast-like symbionts; finally causing brown planthopper ovum to be difficult to hatch or filial generation brown planthopper is difficult to survive ", this will become the important new tool of the one of preventing and treating brown planthopper from now on.
Summary of the invention
Fungicide toyokamycin & tetramycin P & Tetrin B & tetramycin A is used for the vertical transmission of Yeast-like symbionts between generation suppressed or stop in brown planthopper female adult pest body by the present invention, thus reduces brown planthopper egg laying amount, egg hatching rate or make filial generation brown planthopper effectively control brown planthopper because scarce bacterium cannot survive.Described toyokamycin & tetramycin P & Tetrin B & tetramycin A by streptomyces diastatochromogenes (
streptomyces diastatochromogenes) D synthesis.The present invention is that effective control of Brown Planthopper provides a kind of new thinking and countermeasure.
Streptomyces diastatochromogenes of the present invention (
s. diastatochromogenes), name as streptomyces diastatochromogenes D.This bacterial strain in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation address, preservation date on May 25th, 2007, preservation registration number CGMCC No. 2060.
The object of the invention is to develop immunity to drugs to conventional chemical pesticides for brown planthopper, cause the deficiency that chemopreventive effects obviously declines, a kind of fungicide toyokamycin & tetramycin P & Tetrin B & tetramycin A that utilizes is provided to suppress the vertical transmission of Yeast-like symbionts between generation in brown planthopper female adult pest body, and then the new approaches causing filial generation brown planthopper cannot survive because lacking bacterium; Another object of the present invention there is provided a kind of new method of preventing and treating brown planthopper.
The object of the invention is achieved through the following technical solutions:
By fungicide 27% toyokamycin & tetramycin P & Tetrin B & tetramycin A(wherein toyokamycin mass percent 5.58%, tetramycin P mass percent 7.09%, Tetrin B mass percent 4.44%, tetramycin A mass percent 9.64%) for the control of brown planthopper filial generation.
Beneficial effect of the present invention: the invention provides a kind of new method utilizing fungicide to prevent and treat brown planthopper filial generation.
Embodiment
The preparation method of embodiment 1:(27% toyokamycin & tetramycin P & Tetrin B & tetramycin A)
Carry out according to the following steps:
(1) activation culture of bacterial classification: moving to being separated the streptomyces diastatochromogenes D bacterial classification preserved in vitro on Gause I inclined-plane, under 28 DEG C ± 1 DEG C condition, being cultured to test tube slant and covering with spore;
(2) fermented and cultured: fermentation culture is containing soybean cake powder 20g, corn flour 15g in every 1000mL, wheat bran 10g, NH
4nO
33g, (NH
4)
2sO
45g, CaCO
35g, MgSO
41g, sterilizing, to be cooled to 40 DEG C, aseptically inoculate a metal platinum filament ring streptomyces diastatochromogenes D spore in fermentation culture, under 28 DEG C ± 1 DEG C condition, cultivate 5 days;
(3) fermentation product extracts: collected by centrifugation zymotic fluid after fermentation, add equal-volume extraction into ethyl acetate, take off layer, use equal-volume extracting n-butyl alcohol again, get upper strata, be concentrated into medicinal extract under vacuum, toyokamycin & tetramycin P & Tetrin B & tetramycin A is comprised in this medicinal extract, dissolve medicinal extract with running water, being mixed with final concentration is 27% toyokamycin & tetramycin P & Tetrin B & tetramycin A fungicide.
The application of embodiment 2:(27% toyokamycin & tetramycin P & Tetrin B & tetramycin A fungicide in brown planthopper control)
Carry out according to the following steps:
(1) tillering and jointing stage TN1 rice plant, removes part root and base portion leaf sheath afterwash, naturally dries;
(2) compare with clear water, 0.04g/L Imidacloprid liquid is treated to positive control, spraying final concentration is 27% toyokamycin & tetramycin P & Tetrin B & tetramycin A fungicide, require that liquid Pcnten-1 yne-4 is even, coverage rate is high, amount of liquid medicine is suitable, does not produce loss and is advisable, naturally dry with moistening target surface;
(3) the rice seedling stem after step (2) process puts into the Boiling tube injecting 20ml paddy rice culture fluid in advance, each test tube rice seedlings, puberty consistent brown planthopper 1 nymph in age 1 is in vitro put into respectively at often propping up after 12 h, two-layer gauze sealing, each process repetition 150 times, after 1 h, observes invisible spectro nymph one by one, replace physically impaired polypide, ensure in vitro do not have because of physical damnification and the brown planthopper nymph of death.
(4) brown planthopper from 1 age nymph all temperature 26 ± 1 DEG C, humidity: 70-80%, light application time 16 h, interlunation 8 h the indoor cultivation of artificial climate.Observe every day and record the situation of growing of brown planthopper.The brown planthopper adult male and female of having sprouted wings in above-mentioned different disposal group are matched one by one, and corresponding access is equipped with in the Boiling tube of the rice seedling of its alignment processing group, two-layer gauze sealing.Observe the survival condition of adult every day, and change the fresh rice seedling through corresponding chemicals treatment, utilize binocular microscope to carry out microscopy to the rice seedling replaced out, record the index of correlation of laying eggs on the same day.After the rice seedling microscopy that adult stage changes, put it to new in vitro continuation and be cultured to pieces of an egg hatching, observe every day and add up hatching amount, incubation rate, hatching total amount etc.
(5) ovum getting just hatching carries out the mensuration of fungal component quantity, assay method is: 50, the brown planthopper ovum getting each processed group respectively under stereoscope, with the alcohol disinfecting of 75%, rinsed with sterile water, puts into 1.5ml centrifuge tube, adds the physiological saline 10 μ l of 0.9%, homogenate, then get 1 μ l homogenate drop on blood cell counting plate, close the lid fragmentation, under microscope (400 ×), add up fungal component quantity.
Embodiment 3:(27% toyokamycin & tetramycin P & Tetrin B & tetramycin A fungicide is to the preventive and therapeutic effect of brown planthopper nymph and adult)
Carry out according to the following steps:
(1) tillering and jointing stage TN1 rice plant, removes part root and base portion leaf sheath afterwash, naturally dries;
(2) compare with clear water, spraying final concentration is 27% toyokamycin & tetramycin P & Tetrin B & tetramycin A, require that liquid Pcnten-1 yne-4 is even, coverage rate is high, amount of liquid medicine is suitable, do not produce loss with moistening target surface to be advisable, naturally dry;
(3) the rice seedling stem after step (2) process puts into the Boiling tube injecting 20ml paddy rice culture fluid in advance, often pipe 3 rice seedlings, access 3 brown planthopper nymph in age (or the brachypterism female adult pest of just having sprouted wings) 30, to spray the rice seedlings process after clear water as negative control, spray 0.04g/L Imidacloprid liquid and be treated to positive control, each process repeats for 3 times;
(4) within 4 days, brown planthopper lethality is added up after treatment.
Test example 1:(toyokamycin & of the present invention tetramycin P & Tetrin B & tetramycin A and conventional chemical pesticide control Piglet s colibacillosis)
Result of the test is in table 1.As can be seen from Table 1, toyokamycin & tetramycin P & Tetrin B & tetramycin A obviously inhibits brown planthopper egg laying amount, the quantity of ovum endosymbiosis bacterium and the incubation rate of ovum.
Table 1 toyokamycin & tetramycin P & Tetrin B & tetramycin A is on the impact of ovum fungal component quantity, egg hatching rate and egg laying amount
The Piglet s colibacillosis of test example 2:(toyokamycin & tetramycin P & Tetrin B & tetramycin A and conventional chemical pesticide control brown planthopper nymph and adult)
Result of the test is in table 2.As seen from Table 2, toyokamycin & tetramycin P & Tetrin B & tetramycin A is utilized to prevent and treat the effect of brown planthopper nymph and adult all be not as good as Imidacloprid.
Table 2 toyokamycin & tetramycin P & Tetrin B & tetramycin A is to the control efficiency of brown planthopper nymph and adult
Imidacloprid target is brown planthopper, and bactericide toyokamycin & tetramycin P & Tetrin B & tetramycin A target is for Yeast-like symbionts in brown planthopper body, so toyokamycin & tetramycin P & Tetrin B & tetramycin A prevents and treats the effect of brown planthopper nymph and adult all be not as strong as Imidacloprid.But, after toyokamycin & tetramycin P & Tetrin B & tetramycin A process, brown planthopper ovum endosymbiosis bacterium quantity, egg hatching rate and average egg laying amount all significantly decline, effect is stronger than Imidacloprid, effectively improves the control efficiency of filial generation brown planthopper.Main cause is that bactericide toyokamycin & tetramycin P & Tetrin B & tetramycin A stops or inhibits Yeast-like symbionts to enter ovary tissue from the hemolymph of brown planthopper female adult pest, thus make brown planthopper genital system influenced, egg laying amount reduces, the ovum of output also causes incubation rate significantly to reduce because lacking fungal component, thus effectively controls the generation in brown planthopper time generation.
Use toyokamycin & tetramycin P & Tetrin B & tetramycin A of the present invention effectively can improve the control efficiency of filial generation brown planthopper.The prevention and controls of relative popularization insect-proof rice kind and use of insecticide control brown planthopper, brown planthopper of the present invention prevents and treats the problem that new method effectively can avoid brown planthopper water resistant rice varieties and anti-insecticide; The fungicide that the present invention uses not directly to brown planthopper generation effect, but passes through the Yeast-like symbionts prevention and control brown planthopper indirectly in its body, thus safer to people, animal, fish, beneficial insect etc.
Claims (4)
1. utilize fungicide to prevent and treat a new method for brown planthopper filial generation, the mass percent of described fungicide toyokamycin & tetramycin P & Tetrin B & tetramycin A is 27%-54%.
2. new method according to claim 1, obtained by following methods: fungicide 27% toyokamycin & tetramycin P & Tetrin B & tetramycin A is used for stoping or suppress Yeast-like symbionts to enter ovary tissue from the hemolymph of brown planthopper female adult pest, thus make brown planthopper genital system influenced, egg laying amount reduces, the ovum of output also causes incubation rate significantly to reduce because lacking fungal component, thus effectively controls the generation in brown planthopper time generation.
3. fungicide toyokamycin & tetramycin P & Tetrin B & tetramycin A according to claim 2, is synthesized by streptomyces diastatochromogenes D.
4. fungicide according to claim 2, is obtained by following methods:
(1) activation culture of bacterial classification: move in vitro on Gause I inclined-plane by being separated the streptomyces diastatochromogenes D bacterial classification preserved, under 28 DEG C ± 1 DEG C condition, be cultured to test tube slant and cover with spore, described streptomyces diastatochromogenes D is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation address, preservation date on May 25th, 2007, preservation registration number CGMCC No. 2060;
(2) fermented and cultured: fermentation culture is containing soybean cake powder 20g, corn flour 15g in every 1000mL, wheat bran 10g, NH
4nO
33g, (NH
4)
2sO
45g, CaCO
35g, MgSO
41g, sterilizing, to be cooled to 40 DEG C, aseptically inoculate a metal platinum filament ring streptomyces diastatochromogenes D spore in fermentation culture, under 28 DEG C ± 1 DEG C condition, cultivate 5 days;
(3) fermentation product extracts: collected by centrifugation zymotic fluid after fermentation, add equal-volume extraction into ethyl acetate, take off layer, use equal-volume extracting n-butyl alcohol again, get upper strata, be concentrated into medicinal extract under vacuum, toyokamycin & tetramycin P & Tetrin B & tetramycin A is comprised in this medicinal extract, medicinal extract is dissolved with running water, being mixed with final concentration is 27% toyokamycin & tetramycin P & Tetrin B & tetramycin A fungicide (wherein toyokamycin mass percent 5.58%, tetramycin P mass percent 7.09%, Tetrin B mass percent 4.44%, tetramycin A mass percent 9.64%).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510193541.5A CN104782639A (en) | 2015-04-23 | 2015-04-23 | Novel method for preventing and controlling nilaparvata lugens filial generation by using fungicide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510193541.5A CN104782639A (en) | 2015-04-23 | 2015-04-23 | Novel method for preventing and controlling nilaparvata lugens filial generation by using fungicide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104782639A true CN104782639A (en) | 2015-07-22 |
Family
ID=53548196
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510193541.5A Pending CN104782639A (en) | 2015-04-23 | 2015-04-23 | Novel method for preventing and controlling nilaparvata lugens filial generation by using fungicide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104782639A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961013A (en) * | 2010-03-19 | 2011-02-02 | 中国计量学院 | Application of toyocamycin to controlling tomato gray mold |
| CN103960288A (en) * | 2014-03-31 | 2014-08-06 | 中国计量学院 | Application of mixed formula preparation to control on nilaparvata lugens stal |
| CN104430407A (en) * | 2014-12-18 | 2015-03-25 | 中国计量学院 | Application of tetramycin P in inhibition of rhizoctonia solani kuhn of cucumbers and botrytis cinerea |
| CN104531798A (en) * | 2014-12-18 | 2015-04-22 | 中国计量学院 | Method for improving output of tetraene macrolide antibiotics |
-
2015
- 2015-04-23 CN CN201510193541.5A patent/CN104782639A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961013A (en) * | 2010-03-19 | 2011-02-02 | 中国计量学院 | Application of toyocamycin to controlling tomato gray mold |
| CN103960288A (en) * | 2014-03-31 | 2014-08-06 | 中国计量学院 | Application of mixed formula preparation to control on nilaparvata lugens stal |
| CN104430407A (en) * | 2014-12-18 | 2015-03-25 | 中国计量学院 | Application of tetramycin P in inhibition of rhizoctonia solani kuhn of cucumbers and botrytis cinerea |
| CN104531798A (en) * | 2014-12-18 | 2015-04-22 | 中国计量学院 | Method for improving output of tetraene macrolide antibiotics |
Non-Patent Citations (3)
| Title |
|---|
| 吕仲贤等: "共生菌对褐飞虱生长发育和生殖的影响", 《植物保护学报》 * |
| 白旭等: "类酵母共生菌与灰飞虱卵巢发育和产卵量的关系", 《植物保护学报》 * |
| 陈列忠等: "共生菌在褐飞虱防治中的应用", 《农药》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Roy | Jeersanidhi Memorial Award Lecture-Mycological problems of crude herbal drugs-Overview and challenges-AK. ROY | |
| CN104818216A (en) | Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape | |
| CN109182176A (en) | One plant of Siam bacillus and its application in prevention and treatment tomato root-knot eelworm disease | |
| CN104130958A (en) | Bacillus subtilis and application of bacillus subtilis in preventing and curing peony root-knot nematode | |
| CN104845624A (en) | Natural plant biological composite nutrient solution for killing nematode and preparation method thereof | |
| CN105733991A (en) | Bacillus amyloliquefaciens strain and application thereof in preventing and controlling powdery mildew of tobacco | |
| CN105039167A (en) | Beauveria bassiana DSXJ-07 and application thereof | |
| CN101965848B (en) | Biogenic pesticide for efficiently preventing and controlling main diseases of Camellia oleifera and application thereof | |
| CN103503930A (en) | Botanical pesticide composition and application thereof | |
| CN101444231B (en) | Schizophyllum commune protein extract, and preparation method and application thereof | |
| CN106478275A (en) | A kind of composite pesticide of preventing and treating tobacco diseases and preparation method thereof | |
| CN108823140A (en) | A kind of withered bioconversion microbial bacterial agent of paulownia and preparation method thereof for preventing and treating yellow twig | |
| CN101974462A (en) | Bacillus subtilis for preventing and controlling Chinese herbal medicine soil-borne diseases and preparation method of bactericide thereof | |
| CN105061078A (en) | Health bio-organic fertilizer and preparation method thereof | |
| CN104823990A (en) | Use of fungicide in prevention and control of brown planthopper filial generation | |
| CN106222121A (en) | A kind of bacillus megaterium bacterial strain, biocontrol agent and preparation method and application | |
| CN103004894A (en) | Biopesticide for preventing and treating powdery mildew of melons and fruits and preparation method of biopesticide | |
| CN103931665B (en) | A kind of mixing formula preparation preventing and treating brown planthopper | |
| CN104738051A (en) | Novel method for preventing and controlling brown planthopper | |
| CN105112315A (en) | Endophyte strain Alcaligenes faecalis used for biocontrol of tobacco mosaic virus | |
| CN104782639A (en) | Novel method for preventing and controlling nilaparvata lugens filial generation by using fungicide | |
| CN102176831A (en) | A process for the production of a storage stable fungicidal extract of glycyrrhiza glabra for the control of phytopathogenic fungi and other plant diseases | |
| CN103960287B (en) | A kind of mixing formula preparation preventing and treating brown planthopper | |
| CN103960288B (en) | The application of a kind of mixing formula preparation in control brown planthopper | |
| CN103947684B (en) | The application of a kind of mixing formula preparation in brown planthopper control |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150722 |
|
| WD01 | Invention patent application deemed withdrawn after publication |