CN103918701B - A kind of taking is opposed surely and the preparation method of Imidacloprid mixing formula preparation - Google Patents
A kind of taking is opposed surely and the preparation method of Imidacloprid mixing formula preparation Download PDFInfo
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- CN103918701B CN103918701B CN201410176975.XA CN201410176975A CN103918701B CN 103918701 B CN103918701 B CN 103918701B CN 201410176975 A CN201410176975 A CN 201410176975A CN 103918701 B CN103918701 B CN 103918701B
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Abstract
The present invention relates to technical field of biological control, particularly relate to a kind of steady and Imidacloprid mixing formula preparation by enemy preventing and treating brown planthopper.The present invention will (active ingredient mass percent be 75% by the steady water dispersible granules of enemy, wherein oxime bacterium ester mass percent is 25%, Tebuconazole mass percent is 50%) and imidacloprid water dispersible granule (active ingredient mass percent is 70%) be made into mixing formula, for the control of brown planthopper.The most significant feature of the present invention can obviously reduce utilization of pesticides amount with this mixing formula control brown planthopper, effectively improves the control efficiency of brown planthopper, delay the drug-fast development of brown planthopper.
Description
Technical field
The present invention relates to technical field of biological control, particularly relate to a kind of steady and Imidacloprid mixing formula preparation by enemy preventing and treating brown planthopper.
Background technology
Brown planthopper (Nilaparvatalugens
) be one of important pests of paddy rice, mainly to cause harm rice plant to sting to inhale phloem sap and propagate the mode of Rice Virus.There will be large-area rice strain withered lodging during the large generation of insect pest, cause grain drop in production, even have no harvest.In production, chemical pesticide is the Main Means of control brown planthopper.But because a large amount of frequent of chemical insecticide uses, brown planthopper creates pesticide resistance to many chemical insecticides, causes control efficiency obviously to decline.Therefore, investigation and application reduce Brown Planthopper occur and the new tool that causes harm extremely urgent.
The symbiosis of insect and microorganism is a kind of ubiquitous phenomenon, and both define inseparable relation during evolution.Insect is that fungal component provides stable microhabitat and nutrition, and insect relies on some the important nutrient component lacked in fungal component purified diet, the metabolic detoxification of fungal component to insect also has certain effect simultaneously, as synthesized important nutrient component for insect, participate in nitrogen cycle, allogenic material etc. in degraded insect bodies.Disappearance fungal component can directly cause insect growth to breed to be obstructed, lethality rising etc.Research shows, also there is multiple Yeast-like symbionts in brown planthopper body in stomach fat body, Parasaccharomyces Ascomycotina (Ascomycotina), gang pyrenomycetes (pyrenomycetes), and Candida belongs to.Brown planthopper Yeast-like symbionts can be host's synthesis and provides the multiple nutritional components such as essential amino acid, steroid substance and protein, compensate for the imbalance that brown planthopper takes food trophic structure, ensure that normal growth and the breeding of brown planthopper; Yeast-like endosymbiote disappearance can cause brown planthopper growth retardation, arrest of development, even death.Since brown planthopper and Yeast-like symbionts relation in growth and breeding is so tight, controls brown planthopper by the means of " suppress Yeast-like symbionts thus indirectly prevent and treat brown planthopper " and occur and reduce that it causes harm will be a kind of important means of preventing and treating brown planthopper from now on.Because that suppresses that the fungal component in brown planthopper body not only can be direct or indirect reaches the object controlling brown planthopper, but also can effectively improve current insecticide control efficiency, delay the drug-fast development of brown planthopper.
Summary of the invention
The present invention is surely mixed with into new formula with common insecticides Imidacloprid according to certain ratio by taking enemy, and have studied this formula to the inhibitory action of Yeast-like symbionts in brown planthopper body and the insecticidal effect to brown planthopper.This invention is that effective control of Brown Planthopper provides new more efficiently method.
The object of the invention is to develop immunity to drugs to conventional chemical pesticides for brown planthopper, cause the deficiency that chemopreventive effects obviously declines, there is provided a kind of utilization to take enemy surely to suppress Yeast-like symbionts growth in brown planthopper body, and then cause the weak even dead new approaches of brown planthopper; Another object of the present invention there is provided a kind of new method and the new medicament of preventing and treating brown planthopper.
The object of the invention is achieved through the following technical solutions:
Will by enemy steady water dispersible granules (active ingredient mass percent is 75%, wherein oxime bacterium ester mass percent is 25%, Tebuconazole mass percent is 50%) and imidacloprid water dispersible granule (active ingredient mass percent is 70%) be made into mixing formula, for the control of brown planthopper.
Beneficial effect of the present invention: the invention provides a kind of utilization by opposing new method that is steady and Imidacloprid mixing formula preparation control brown planthopper.
Embodiment
Embodiment 1:(takes the preparation opposing steady and Imidacloprid mixing formula preparation)
The steady water dispersible granules of enemy and imidacloprid water dispersible granule is taken to produce by Beyer Co., Ltd, dissolve with running water respectively by imidacloprid water dispersible granule with by the steady water dispersible granules of enemy, mixing, being made into final concentration is that 0.3g/L takes the steady and 0.01g/L Imidacloprid mixing formula preparation of enemy.
Embodiment 2:(takes and opposes the steady and application of Imidacloprid mixing formula preparation in control brown planthopper nymph)
Carry out according to the following steps:
(1) tillering and jointing stage TN1 paddy rice, removes part root and base portion leaf sheath afterwash, naturally dries;
(2) compare with clear water, spraying final concentration is that 0.3g/L takes the steady and 0.01g/L Imidacloprid mixing formula of enemy, and require that liquid Pcnten-1 yne-4 is even, coverage rate is high, and amount of liquid medicine suitably, does not produce loss with moistening target surface and is advisable, naturally dry;
(3) rice seedling after step (2) process puts the Boiling tube that people injects 20ml paddy rice culture fluid in advance, often pipe 3 rice seedlings, access 3 age brown planthopper nymph 30, to spray the rice seedlings process after clear water as negative control, spray 0.04g/L Imidacloprid liquid and be treated to positive control, each process repeats for 3 times;
(4) difference 1 day after treatment, measures brown planthopper parachorium bacterium quantity for 2 days and 4 days, and adds up brown planthopper lethality.Wherein brown planthopper parachorium bacterium quantitative measurement method is: get the brown planthopper 3 survived in each process respectively, with the alcohol disinfecting of 75%, rinsed with sterile water, remove wing, head and chest, belly is put into 1.5ml centrifuge tube, add the physiological saline 50 μ l of 0.9%, homogenate, then get 2 μ l homogenate drops on blood cell counting plate, close the lid fragmentation, under microscope (16 × 40), add up fungal component quantity.
Embodiment 3:(takes and opposes the steady and application of Imidacloprid mixing formula in control brown planthopper female adult pest)
Carry out according to the following steps:
(1) tillering and jointing stage TN1 paddy rice, removes part root and base portion leaf sheath afterwash, naturally dries;
(2) compare with clear water, spraying final concentration is that 0.3g/L takes the steady and 0.01g/L Imidacloprid mixing formula of enemy, and require that liquid Pcnten-1 yne-4 is even, coverage rate is high, and amount of liquid medicine suitably, does not produce loss with moistening target surface and is advisable, naturally dry;
(3) rice seedling after step (2) process puts into the Boiling tube injecting 20ml paddy rice culture fluid in advance, often pipe 4 rice seedlings, the brachypterism female adult pest 30 that access has just been sprouted wings, to spray the rice seedlings process after clear water as negative control, spray 0.08g/L Imidacloprid liquid and be treated to positive control, each process repeats for 3 times;
(4) difference 1 day after treatment, within 2 days and 4 days, measure brachypterism female worm brown planthopper parachorium bacterium quantity, and add up brown planthopper lethality, wherein brown planthopper parachorium bacterium quantitative measurement method is: get the female worm of the brachypterism of surviving in each process 3 respectively, with the alcohol disinfecting of 75%, rinsed with sterile water, remove wing, head and chest, belly is put into 1.5ml centrifuge tube, add the physiological saline 50 μ l of 0.9%, homogenate, then get 2 μ l homogenate drops on blood cell counting plate, close the lid fragmentation, under microscope (16 × 40), add up fungal component quantity.
Test example: (of the present invention take the steady and Imidacloprid pharmaceutical formulation of enemy and conventional chemical pesticide control Piglet s colibacillosis)
Result of the test is in table 1 and table 2.As seen from Table 1 and Table 2, utilize the quantity of taking the steady and Imidacloprid mixing formula preparation of enemy obviously to inhibit brown planthopper parachorium bacterium, thus cause brown planthopper lethality to rise.
Table 1 takes the steady and Imidacloprid mixing formula preparation of enemy to the control efficiency of brown planthopper nymph
Table 2 takes the steady and Imidacloprid mixing formula preparation of enemy to the control efficiency of brown planthopper female adult pest
Steady and the control efficiency of Imidacloprid decrement formula to brown planthopper nymph and female adult pest of enemy is taken to be better than conventional chemical liquid or similar.Use and of the present inventionly take the steady and Imidacloprid mixing formula control brown planthopper of enemy can obviously reduce utilization of pesticides amount, effectively the control efficiency of raising brown planthopper, delays the drug-fast development of brown planthopper.
Claims (2)
1. take a preparation method for the steady and Imidacloprid mix preparation of enemy, this mix preparation comprises takes the steady water dispersible granules of enemy and imidacloprid water dispersible granule, describedly takes the weight ratio of opposing steady water dispersible granules and imidacloprid water dispersible granule to be 30:1-10:1.
2. the preparation method of mix preparation according to claim 1, is obtained by following methods: take the steady water dispersible granules of enemy and imidacloprid water dispersible granule to produce by Beyer Co., Ltd; Described active ingredient mass concentration of taking the steady water dispersible granules of enemy is 75%, and wherein oxime bacterium ester accounts for 25%, and Tebuconazole accounts for 50%; The active ingredient mass concentration of described imidacloprid water dispersible granule is 70%; By imidacloprid water dispersible granule with take the steady water dispersible granules of enemy to dissolve with running water respectively, mixing, being made into final concentration is that 0.3g/L takes the steady and 0.01g/L Imidacloprid mix preparation of enemy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410176975.XA CN103918701B (en) | 2014-04-25 | 2014-04-25 | A kind of taking is opposed surely and the preparation method of Imidacloprid mixing formula preparation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410176975.XA CN103918701B (en) | 2014-04-25 | 2014-04-25 | A kind of taking is opposed surely and the preparation method of Imidacloprid mixing formula preparation |
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| Publication Number | Publication Date |
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| CN103918701A CN103918701A (en) | 2014-07-16 |
| CN103918701B true CN103918701B (en) | 2016-03-09 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104630081A (en) * | 2015-01-22 | 2015-05-20 | 中国计量学院 | Yeast-like symbiote and use thereof |
| CN104738051A (en) * | 2015-04-23 | 2015-07-01 | 中国计量学院 | Novel method for preventing and controlling brown planthopper |
| CN104823767A (en) * | 2015-04-23 | 2015-08-12 | 中国计量学院 | Application of nativo to control over filial generations of brown planthopper |
| CN105010355A (en) * | 2015-06-30 | 2015-11-04 | 郑州郑氏化工产品有限公司 | Antimicrobial insecticidal composition used for paddy rice field and preparation method of its water dispersible granules |
Citations (5)
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| WO2003015518A1 (en) * | 2001-08-13 | 2003-02-27 | E.I. Du Pont De Nemours And Company | Method for controlling particular insect pests by applying anthranilamide compounds |
| CN1546689A (en) * | 2003-12-12 | 2004-11-17 | 南京农业大学 | Molecular detecting method of resistance of Nilaparvata lugens to imidacloprid target |
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| CN102860211A (en) * | 2012-09-29 | 2013-01-09 | 国兴农现代农业发展股份有限公司 | Pollution-free greenhouse watermelon growing method |
| CN104041510A (en) * | 2011-07-19 | 2014-09-17 | 陕西上格之路生物科学有限公司 | Bactericidal composition containing coumoxystrobin and triazole bactericide |
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2014
- 2014-04-25 CN CN201410176975.XA patent/CN103918701B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003015518A1 (en) * | 2001-08-13 | 2003-02-27 | E.I. Du Pont De Nemours And Company | Method for controlling particular insect pests by applying anthranilamide compounds |
| CN1546689A (en) * | 2003-12-12 | 2004-11-17 | 南京农业大学 | Molecular detecting method of resistance of Nilaparvata lugens to imidacloprid target |
| CN104041510A (en) * | 2011-07-19 | 2014-09-17 | 陕西上格之路生物科学有限公司 | Bactericidal composition containing coumoxystrobin and triazole bactericide |
| CN102349534A (en) * | 2011-08-26 | 2012-02-15 | 河北北方学院 | Imidacloprid and pymetrozine compound microcapsule suspension and preparation method thereof |
| CN102860211A (en) * | 2012-09-29 | 2013-01-09 | 国兴农现代农业发展股份有限公司 | Pollution-free greenhouse watermelon growing method |
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| 75%拿敌稳水分散粒剂对西瓜病害的防效研究;史顺荣等;《现代农业科技》;20101130(第22期);全文 * |
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| CN103918701A (en) | 2014-07-16 |
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