CN104822830B - 内含肽修饰的蛋白酶、其制备方法和工业用途 - Google Patents

内含肽修饰的蛋白酶、其制备方法和工业用途 Download PDF

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CN104822830B
CN104822830B CN201380063222.1A CN201380063222A CN104822830B CN 104822830 B CN104822830 B CN 104822830B CN 201380063222 A CN201380063222 A CN 201380063222A CN 104822830 B CN104822830 B CN 104822830B
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seq
intein
protease
prosavinase
detergent
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CN104822830A (zh
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R·M·莱布
B·沈
G·拉扎尔
H·德拉维加
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Gu Wanda
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CN201380063222.1A 2012-10-03 2013-10-03 内含肽修饰的蛋白酶、其制备方法和工业用途 Expired - Fee Related CN104822830B (zh)

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US201261744863P 2012-10-03 2012-10-03
US61/744,863 2012-10-03
US201361783424P 2013-03-14 2013-03-14
US61/783,424 2013-03-14
PCT/US2013/063304 WO2014055782A1 (en) 2012-10-03 2013-10-03 Intein-modified proteases, their production and industrial applications

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Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
US20170114091A1 (en) * 2014-03-28 2017-04-27 Novozymes A/S Resolubilization of protein crystals at low ph
US10385326B2 (en) * 2014-05-30 2019-08-20 Novozymes A/S Variants of GH family 11 xylanase and polynucleotides encoding same
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
EP3212778B1 (en) * 2014-10-28 2019-08-28 Agrivida, Inc. Methods and compositions for stabilizing trans-splicing intein modified proteases
EP3365356B1 (en) 2015-10-23 2023-06-28 President and Fellows of Harvard College Nucleobase editors and uses thereof
RU2619217C1 (ru) * 2015-12-04 2017-05-12 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Температурочувствительный мутантный интеин для нерастворимой экспрессии предшественника целевого белка
CN105420220B (zh) * 2016-01-26 2018-10-09 华东理工大学 一种天冬氨酸类蛋白酶及其编码基因与应用
CN105925596A (zh) * 2016-02-23 2016-09-07 上海交通大学 基于内含肽的药用重组蛋白的合成方法
US11001787B2 (en) 2016-06-23 2021-05-11 Novozymes A/S Use of enzymes, composition and method for removing soil
JP7231935B2 (ja) 2016-08-03 2023-03-08 プレジデント アンド フェローズ オブ ハーバード カレッジ アデノシン核酸塩基編集因子およびそれらの使用
EP3497214B1 (en) 2016-08-09 2023-06-28 President and Fellows of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3526320A1 (en) 2016-10-14 2019-08-21 President and Fellows of Harvard College Aav delivery of nucleobase editors
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
GB201702938D0 (en) 2017-02-23 2017-04-12 Univ Southampton Methods of generating and screening compartmentalised peptides libraries
WO2018165631A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Cancer vaccine
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
CN111757937A (zh) 2017-10-16 2020-10-09 布罗德研究所股份有限公司 腺苷碱基编辑器的用途
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
EP3752179A4 (en) 2018-02-15 2021-10-06 Duke University GENE EDITING USING A UNIVERSAL GENOMIC ENGINEERING TECHNOLOGY INDEPENDENT OF HOMOLOGY
EP3546558A1 (en) * 2018-03-28 2019-10-02 The Procter & Gamble Company Laundry detergent composition
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
CN109456956B (zh) * 2018-11-06 2022-03-18 济南百斯杰生物工程有限公司 啤酒加工过程大麦副产物酶解处理用酶组合物及处理方法
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
MX2021011426A (es) 2019-03-19 2022-03-11 Broad Inst Inc Metodos y composiciones para editar secuencias de nucleótidos.
US20220204975A1 (en) 2019-04-12 2022-06-30 President And Fellows Of Harvard College System for genome editing
EP3956349A1 (en) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN110172451A (zh) * 2019-05-05 2019-08-27 昆明理工大学 一种高通量分离噬菌体的方法
WO2020252455A1 (en) 2019-06-13 2020-12-17 The General Hospital Corporation Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
EP4010474A1 (en) 2019-08-08 2022-06-15 The Broad Institute, Inc. Base editors with diversified targeting scope
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CN110846299B (zh) * 2019-11-22 2021-09-24 江南大学 一种前导肽突变体及其在角蛋白酶生产中的应用
US20230108687A1 (en) 2020-02-05 2023-04-06 The Broad Institute, Inc. Gene editing methods for treating spinal muscular atrophy
EP4118206A1 (en) 2020-03-11 2023-01-18 The Broad Institute Inc. Stat3-targeted base editor therapeutics for the treatment of melanoma and other cancers
WO2021222318A1 (en) 2020-04-28 2021-11-04 The Broad Institute, Inc. Targeted base editing of the ush2a gene
MX2022014008A (es) 2020-05-08 2023-02-09 Broad Inst Inc Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo.
CN116670154A (zh) 2020-07-24 2023-08-29 总医院公司 增强的病毒样颗粒及使用其递送至细胞的方法
AU2021350835A1 (en) 2020-09-24 2023-04-27 President And Fellows Of Harvard College Prime editing guide rnas, compositions thereof, and methods of using the same
JP2024503437A (ja) 2021-01-11 2024-01-25 ザ ブロード インスティテュート,インコーポレーテッド プライム編集効率及び精度を向上させるためのプライム編集因子バリアント、構築物、及び方法
CN113403209B (zh) * 2021-07-30 2022-08-26 西南大学 天冬氨酸蛋白酶基因在改良球孢白僵菌菌株中的应用
AU2022325166A1 (en) 2021-08-06 2024-02-08 President And Fellows Of Harvard College Improved prime editors and methods of use
US20240417719A1 (en) 2021-10-25 2024-12-19 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase
GB2630191A (en) 2021-12-03 2024-11-20 Broad Inst Inc Self-assembling virus-like particles for delivery of prime editors and methods of making and using same
WO2023205687A1 (en) 2022-04-20 2023-10-26 The Broad Institute, Inc. Improved prime editing methods and compositions
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
US20250376674A1 (en) 2022-06-23 2025-12-11 Prime Medicine, Inc. Split prime editors
CN115925830B (zh) * 2022-08-15 2023-11-07 广州市乾相生物科技有限公司 一种内含肽变体及其在生物法制备类蛇毒肽前体中的应用
WO2024077267A1 (en) 2022-10-07 2024-04-11 The Broad Institute, Inc. Prime editing methods and compositions for treating triplet repeat disorders
EP4619515A1 (en) 2022-11-17 2025-09-24 The Broad Institute, Inc. Prime editor delivery by aav
CN116731126B (zh) * 2023-01-30 2024-02-23 态创生物科技(广州)有限公司 内含肽ChiATP、内含肽ChiATP-二肽-2融合蛋白及二肽-2的表达方法
WO2024163862A2 (en) 2023-02-03 2024-08-08 The Broad Institute, Inc. Gene editing methods, systems, and compositions for treating spinal muscular atrophy
WO2025165968A1 (en) * 2024-01-31 2025-08-07 Danisco Us Inc. Conditional regulation of protein function in filamentous fungal cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000052155A2 (en) * 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT366877B (de) 1980-07-28 1982-05-10 Voest Alpine Ag Gewaechshaus
US5543302A (en) 1988-05-27 1996-08-06 Solvay Enzymes, Inc. Proteases of altered stability to autolytic degradation
DE4023458A1 (de) * 1989-08-31 1991-03-07 Kali Chemie Ag Neue hochalkalische proteasen
DE69835814T2 (de) 1997-06-04 2007-01-18 Genencor International, Inc., Palo Alto Protease zur gründlichen reinigung und/oder flecken- und filmreduktion und zusammensetzungen damit
WO2000018881A2 (en) * 1998-09-30 2000-04-06 New England Biolabs, Inc. Intein mediated peptide ligation
AU781215B2 (en) * 1998-12-18 2005-05-12 Penn State Research Foundation, The Cyclic peptides
DE60040524D1 (de) * 1999-02-12 2008-11-27 New England Biolabs Inc Intein-vermittelte ligation von exprimierten proteinen
AU6781700A (en) * 1999-08-17 2001-03-13 Health Research Institute Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity
US20040091966A1 (en) * 1999-08-30 2004-05-13 Martin Zeidler Polypeptide regulation by conditional inteins
US6855548B2 (en) * 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US7026526B2 (en) 2000-02-11 2006-04-11 Metabolix, Inc. Multi-gene expression constructs containing modified inteins
CA2445049A1 (en) * 2001-05-04 2002-11-14 Cornell Research Foundation, Inc. Rapidly cleavable sumo fusion protein expression system for difficult to express proteins
CA2472886C (en) * 2002-01-08 2013-05-28 R. Michael Raab Transgenic plants expressing civps or intein modified proteins and related method
US20030167533A1 (en) 2002-02-04 2003-09-04 Yadav Narendra S. Intein-mediated protein splicing
BRPI0313288B1 (pt) 2002-08-09 2018-09-11 Bioresource Int Inc métodos para criar aves domésticas para abate, para melhorar a eficiência da utilização de ração de uma ração animal em aves domésticas para abate e para aumentar a capacidade de digestão de uma ração animal em aves domésticas para abate, e, ração animal
EP1594963B1 (en) 2003-02-07 2007-07-25 Novozymes A/S Proteases
WO2005098043A2 (en) * 2004-03-30 2005-10-20 The President And Fellows Of Harvard College Ligand-dependent protein splicing
CA2567272A1 (en) 2004-05-19 2005-12-01 Agrivida, Inc. Transgenic plants expressing intein modified proteins and associated processes for bio-pharmaceutical production
EP2548954A1 (en) 2006-02-14 2013-01-23 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
US8420387B2 (en) 2009-11-06 2013-04-16 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
EP2608656B1 (en) 2010-08-27 2016-04-27 Agrivida, Inc. Cellulosic processing trait development using a thermoregulated, intein-modified xylanase
BR112013004743A2 (pt) 2010-10-01 2016-06-07 Novozymes As polipeptídeo isolado com atividade de endopeptidase, composição, método para produzir o polipeptídeo, preparar um hidrolisado de proteína, e para preparar um hidrolisado de proteína alimentar, e, usos do polipeptídeo ou da composição, e de uma endopeptidase do tipo tripsina derivada de uma bactéria
ES2618632T3 (es) * 2011-09-28 2017-06-21 Zera Intein Protein Solutions, S.L. Inteínas divididas y usos de éstas
WO2014067084A1 (zh) 2012-10-31 2014-05-08 深圳翰宇药业股份有限公司 一种艾塞那肽的制备方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000052155A2 (en) * 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A Predictive Model of Intein Insertion Site for Use in the Engineering of Molecular Switches;James Apgar et al.;《PLoS ONE》;20120531;第5页右栏第2段,第6页图5 *
Unprecedented Rates and Efficiencies Revealed for New Natural Split Inteins from Metagenomic Sources;Patricia Carvajal-Vallejos et al.;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;20120817;摘要和表1 *
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step;Shao rong Chong et al.;《Nucleic Acids Research》;19981231;5111页左栏第1段和右栏第1段,第5113页右栏第2段 *

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