DE60040524D1 - Intein-vermittelte ligation von exprimierten proteinen - Google Patents

Intein-vermittelte ligation von exprimierten proteinen

Info

Publication number
DE60040524D1
DE60040524D1 DE60040524T DE60040524T DE60040524D1 DE 60040524 D1 DE60040524 D1 DE 60040524D1 DE 60040524 T DE60040524 T DE 60040524T DE 60040524 T DE60040524 T DE 60040524T DE 60040524 D1 DE60040524 D1 DE 60040524D1
Authority
DE
Germany
Prior art keywords
intein
expressed proteins
mediated ligation
ligation
mediated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60040524T
Other languages
English (en)
Inventor
Thomas C Evans
Ming-Qun Xu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
XU MING QUN
New England Biolabs Inc
Original Assignee
XU MING QUN
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/249,543 external-priority patent/US6849428B1/en
Application filed by XU MING QUN, New England Biolabs Inc filed Critical XU MING QUN
Application granted granted Critical
Publication of DE60040524D1 publication Critical patent/DE60040524D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0093—Oxidoreductases (1.) acting on CH or CH2 groups (1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60040524T 1999-02-12 2000-02-02 Intein-vermittelte ligation von exprimierten proteinen Expired - Lifetime DE60040524D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/249,543 US6849428B1 (en) 1997-03-05 1999-02-12 Intein-mediated protein ligation of expressed proteins
PCT/US2000/002764 WO2000047751A1 (en) 1999-02-12 2000-02-02 Intein-mediated protein ligation of expressed proteins

Publications (1)

Publication Number Publication Date
DE60040524D1 true DE60040524D1 (de) 2008-11-27

Family

ID=22943945

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60040524T Expired - Lifetime DE60040524D1 (de) 1999-02-12 2000-02-02 Intein-vermittelte ligation von exprimierten proteinen

Country Status (4)

Country Link
EP (1) EP1151117B1 (de)
JP (1) JP2003525584A (de)
DE (1) DE60040524D1 (de)
WO (1) WO2000047751A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6781700A (en) * 1999-08-17 2001-03-13 Health Research Institute Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity
US6897285B2 (en) 2002-02-28 2005-05-24 New England Biolabs, Inc. Modified chitin binding domain and uses thereof
CA2523034A1 (en) * 2003-04-28 2004-11-11 Sekisui Chemical Co., Ltd. Method of producing target protein, fused protein and gene thereof, partial sequence protein of intein and gene thereof, expression vector and transformant
WO2009047354A1 (en) * 2007-10-12 2009-04-16 Dsm Ip Assets B.V. Chemo-enzymatic synthesis of a c-terminal thioester of an amino acid or a peptide
AU2013326972B2 (en) 2012-10-03 2019-08-08 Agrivida, Inc. Intein-modified proteases, their production and industrial applications
EP2943573A1 (de) 2013-01-11 2015-11-18 The Texas A&M University System Intein-vermittelte proteinreinigung
DE102015207516A1 (de) 2015-04-23 2016-10-27 Paul-Ehrlich-Institut Bundesamt Für Sera Und Impfstoffe Kopplung von Proteinen von Interesse (POI) mit viralen Vektoren mittels Intein-vermittelten Proteinspleißens
CN106924753B (zh) * 2015-12-30 2021-11-09 北京大学 制备蛋白质-聚氨基酸环状偶联物的方法
CN107312791B (zh) * 2017-08-11 2020-04-14 湖南农业大学 双拷贝eip表达载体及其构建方法和应用
TWI671314B (zh) * 2018-06-29 2019-09-11 國立清華大學 自斷蛋白的活性控制及其應用
KR102129377B1 (ko) * 2019-02-21 2020-07-02 성균관대학교산학협력단 특정 특성이 발현된 단백질을 암호화하는 플라스미드를 선별하는 유도 플라스미드 디스플레이 시스템

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834247A (en) * 1992-12-09 1998-11-10 New England Biolabs, Inc. Modified proteins comprising controllable intervening protein sequences or their elements methods of producing same and methods for purification of a target protein comprised by a modified protein
JP4749548B2 (ja) * 1998-09-30 2011-08-17 ニユー・イングランド・バイオレイブズ・インコーポレイテツド インテイン媒介ペプチド連結

Also Published As

Publication number Publication date
EP1151117A1 (de) 2001-11-07
EP1151117A4 (de) 2005-08-10
JP2003525584A (ja) 2003-09-02
EP1151117B1 (de) 2008-10-15
WO2000047751A1 (en) 2000-08-17

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