CN104820054B - 高精度测量猪尿中的莱克多巴胺、克仑特罗、沙丁胺醇含量的方法 - Google Patents
高精度测量猪尿中的莱克多巴胺、克仑特罗、沙丁胺醇含量的方法 Download PDFInfo
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- CN104820054B CN104820054B CN201510238919.9A CN201510238919A CN104820054B CN 104820054 B CN104820054 B CN 104820054B CN 201510238919 A CN201510238919 A CN 201510238919A CN 104820054 B CN104820054 B CN 104820054B
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- ractopamine
- clenbuterol
- urine
- albuterol
- acruracy survey
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- 210000002700 urine Anatomy 0.000 title claims abstract description 43
- YJQZYXCXBBCEAQ-UHFFFAOYSA-N ractopamine Chemical compound C=1C=C(O)C=CC=1C(O)CNC(C)CCC1=CC=C(O)C=C1 YJQZYXCXBBCEAQ-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 229940074095 ractopamine Drugs 0.000 title claims abstract description 36
- NDAUXUAQIAJITI-UHFFFAOYSA-N albuterol Chemical compound CC(C)(C)NCC(O)C1=CC=C(O)C(CO)=C1 NDAUXUAQIAJITI-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 229960001117 clenbuterol Drugs 0.000 title claims abstract description 34
- STJMRWALKKWQGH-UHFFFAOYSA-N clenbuterol Chemical compound CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 STJMRWALKKWQGH-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 229960002052 salbutamol Drugs 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000003480 eluent Substances 0.000 claims abstract description 14
- 239000006228 supernatant Substances 0.000 claims abstract description 12
- 102000009133 Arylsulfatases Human genes 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- 229930182470 glycoside Natural products 0.000 claims abstract description 7
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 7
- 108060007951 sulfatase Proteins 0.000 claims abstract description 7
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical group CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 39
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 15
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims description 13
- KXKVLQRXCPHEJC-UHFFFAOYSA-N acetic acid trimethyl ester Natural products COC(C)=O KXKVLQRXCPHEJC-UHFFFAOYSA-N 0.000 claims description 13
- 238000001514 detection method Methods 0.000 claims description 11
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000000622 liquid--liquid extraction Methods 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 238000000638 solvent extraction Methods 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims description 4
- 239000005695 Ammonium acetate Substances 0.000 claims description 4
- 238000003916 acid precipitation Methods 0.000 claims description 4
- 229940043376 ammonium acetate Drugs 0.000 claims description 4
- 235000019257 ammonium acetate Nutrition 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000013461 design Methods 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 description 5
- 238000000605 extraction Methods 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 102000012740 beta Adrenergic Receptors Human genes 0.000 description 2
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- 239000000839 emulsion Substances 0.000 description 1
- 239000002024 ethyl acetate extract Substances 0.000 description 1
- ANFZRGMDGDYNGA-UHFFFAOYSA-N ethyl acetate;propan-2-ol Chemical compound CC(C)O.CCOC(C)=O ANFZRGMDGDYNGA-UHFFFAOYSA-N 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
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Abstract
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CN201510238919.9A CN104820054B (zh) | 2015-05-12 | 2015-05-12 | 高精度测量猪尿中的莱克多巴胺、克仑特罗、沙丁胺醇含量的方法 |
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CN104820054B true CN104820054B (zh) | 2016-07-06 |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101949898A (zh) * | 2010-08-10 | 2011-01-19 | 上海安谱科学仪器有限公司 | 一种动物源性食品中多种碱性药物残留量的检测方法 |
CN103076416A (zh) * | 2011-11-15 | 2013-05-01 | 南昌大学 | 一种富集养殖家畜尿样中克伦特罗、莱克多巴胺和沙丁胺醇的方法 |
CN104251898A (zh) * | 2014-10-22 | 2014-12-31 | 威海出入境检验检疫局检验检疫技术中心 | 膜透析-液相色谱串联质谱测定畜产品中多种β-受体兴奋剂残留的方法 |
CN104524812A (zh) * | 2014-12-17 | 2015-04-22 | 南京博蕴生物科技有限公司 | 使用固载液液萃取柱从中药材水提液中萃取有效成分的方法 |
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2015
- 2015-05-12 CN CN201510238919.9A patent/CN104820054B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101949898A (zh) * | 2010-08-10 | 2011-01-19 | 上海安谱科学仪器有限公司 | 一种动物源性食品中多种碱性药物残留量的检测方法 |
CN103076416A (zh) * | 2011-11-15 | 2013-05-01 | 南昌大学 | 一种富集养殖家畜尿样中克伦特罗、莱克多巴胺和沙丁胺醇的方法 |
CN104251898A (zh) * | 2014-10-22 | 2014-12-31 | 威海出入境检验检疫局检验检疫技术中心 | 膜透析-液相色谱串联质谱测定畜产品中多种β-受体兴奋剂残留的方法 |
CN104524812A (zh) * | 2014-12-17 | 2015-04-22 | 南京博蕴生物科技有限公司 | 使用固载液液萃取柱从中药材水提液中萃取有效成分的方法 |
Non-Patent Citations (4)
Title |
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Enantioselective determination of alprenolol in human plasma by liquid chromatography with tandem mass spectrometry using cellobiohydrolase chiral stationary phases;Hongliang Jiang et al;《Journal of Chromatography B》;20080729;第872卷(第1-2期);第121-127页 * |
Multi-residue quantification of veterinary drugs in milk with a novel extraction and cleanup technique:Salting out supported liquid extraction (SOSLE);A. Kaufmann et al;《Analytica Chimica Acta》;20140226;第820卷;第56-68页 * |
在线固相萃取-高效液相色谱-质谱法快速测定绵羊尿液中3种轭合态β-受体激动剂;李晓敏 等;《分析化学》;20141215;第42卷(第12期);第1779-1784页 * |
液相色谱串联质谱法测定饲料中盐酸克伦特罗、莱克多巴胺、沙丁胺醇、苯乙醇胺A;顾亮 等;《化学分析计量》;20120120;第21卷(第1期);第37-39页 * |
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Effective date of registration: 20170122 Address after: 543000 the Guangxi Zhuang Autonomous Region Cenxi North Road No. 5 Patentee after: Cenxi Productivity Promotion Center Address before: 543000 the Guangxi Zhuang Autonomous Region, Wuzhou West Ring Road, No. 198 Patentee before: WUZHOU INSTITUTE FOR FOOD AND DRUG CONTROL, GUANGXI ZHUANG AUTONOMOUS REGION |
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Inventor after: Guo Zhenwang Inventor after: Sun Liangguang Inventor after: Chen Xuesong Inventor after: Huang Heng Inventor after: Wu Lan Inventor after: Deng Shuide Inventor after: Li Zhirui Inventor before: Chen Xuesong Inventor before: Guo Zhenwang Inventor before: Huang Heng Inventor before: Wu Lan Inventor before: Deng Shuide Inventor before: Li Zhirui |
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Effective date of registration: 20180426 Address after: 543000 198 West Ring Road, Wuzhou, the Guangxi Zhuang Autonomous Region Patentee after: WUZHOU FOOD AND DRUG INSPECTION INSTITUTE Address before: No. 5 north mountain road, Cenxi, the Guangxi Zhuang Autonomous Region Patentee before: Cenxi Productivity Promotion Center |
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Application publication date: 20150805 Assignee: Cenxi Longyuan Breeding Co.,Ltd. Assignor: WUZHOU FOOD AND DRUG INSPECTION INSTITUTE Contract record no.: X2022450000312 Denomination of invention: A method for high precision measurement of ractopamine, clenbuterol and salbutamol in pig urine Granted publication date: 20160706 License type: Common License Record date: 20221212 |