CN104815349B - Adhesive prepared using giant salamander mucus and preparation method thereof - Google Patents
Adhesive prepared using giant salamander mucus and preparation method thereof Download PDFInfo
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- CN104815349B CN104815349B CN201510154685.XA CN201510154685A CN104815349B CN 104815349 B CN104815349 B CN 104815349B CN 201510154685 A CN201510154685 A CN 201510154685A CN 104815349 B CN104815349 B CN 104815349B
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- giant salamander
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- salamander mucus
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- mucus
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- 210000003097 mucus Anatomy 0.000 title claims abstract description 47
- 239000000853 adhesive Substances 0.000 title claims abstract description 40
- 230000001070 adhesive effect Effects 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 241000157862 Dicamptodontidae Species 0.000 title claims abstract 16
- 230000001954 sterilising effect Effects 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 9
- 239000007787 solid Substances 0.000 claims abstract description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 5
- 238000007710 freezing Methods 0.000 claims description 7
- 230000008014 freezing Effects 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 238000001291 vacuum drying Methods 0.000 claims description 7
- 230000005855 radiation Effects 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 2
- 239000003106 tissue adhesive Substances 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 8
- 238000004026 adhesive bonding Methods 0.000 abstract 1
- 210000000988 bone and bone Anatomy 0.000 abstract 1
- 241001415306 Cryptobranchidae Species 0.000 description 39
- 210000003491 skin Anatomy 0.000 description 10
- 206010052428 Wound Diseases 0.000 description 6
- 208000027418 Wounds and injury Diseases 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 241000269333 Caudata Species 0.000 description 3
- 108010080379 Fibrin Tissue Adhesive Proteins 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241001253208 Andrias davidianus Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 229920001651 Cyanoacrylate Polymers 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 2
- 239000004264 Petrolatum Substances 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 238000009455 aseptic packaging Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000000038 chest Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229940066842 petrolatum Drugs 0.000 description 2
- 235000019271 petrolatum Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 241000395633 Andrias japonicus Species 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 244000227633 Ocotea pretiosa Species 0.000 description 1
- 235000004263 Ocotea pretiosa Nutrition 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000256856 Vespidae Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000011013 aquamarine Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- RPGQNYWRHVRNGS-UHFFFAOYSA-N cyano prop-2-enoate Chemical compound C(#N)OC(C=C)=O.C(#N)OC(C=C)=O RPGQNYWRHVRNGS-UHFFFAOYSA-N 0.000 description 1
- 230000035617 depilation Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000004003 subcutaneous fat Anatomy 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
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- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses adhesive prepared using giant salamander mucus and preparation method thereof, adhesive is the freeze-dried powder of giant salamander mucus, its preparation method is that giant salamander mucus is frozen into solid, then vacuum freezedrying removes moisture removal, dry giant salamander mucus is crushed again, sterilizing, is produced, this method is simple, obtained adhesive bonding performance is strong, tissue adhesive can be directly used as, bonding, bone bonding for wound etc., because the adhesive derives from giant salamander, for pure natural components, security and good biocompatibility, it is degradable, it is with a wide range of applications.
Description
Technical field
The invention belongs to technical field of biological material, and in particular to adhesive and the adhesive prepared by a kind of utilization giant salamander mucus
Preparation method.
Background technology
Giant salamander is commonly called as the giant salamander, is under the jurisdiction of Amphibia Caudata Yin Sai salamanders section, including 23 kinds of category, i.e. giant salamander category and hidden cheek salamander
Category, wherein giant salamander category again can be divided into Andrias japonicus and Chinese giant salamander, Andrias davidianus, be existing maximum, the most precious amphibian in the world, by
The United Nations is included in endangered species protection register, is national two classes protection aqua-marine life.Giant salamander fine and tender taste, delicious flavour,
Containing good protein, abundant amino acid and trace element, nutritive value is high, is described as " ginseng in water " and " soft Huang
Gold ".When giant salamander skin is by environmental stimuli, a large amount of white " milky "s, mucus with special odor can be secreted, are sent out through research
Main component is water (83-86%), protein (12%) in the mucus now secreted, and containing a small amount of polysaccharide (0.48%), is had
Antibacterial, it is anti-oxidant and antitumor the effects such as, cosmetic field is mainly used at present.Because the yield of giant salamander mucus is filled very much
Foot, repeats a large amount of collections, and its utilization rate is low.It would therefore be highly desirable to further be developed to the addition product of giant salamander mucus, giant salamander is improved
Comprehensive value.
The content of the invention
In view of this, an object of the present invention is to provide the adhesive prepared using giant salamander mucus, and bond properties is strong,
Good biocompatibility, it is degradable, and security is good；The second object of the present invention is the preparation method for providing above-mentioned adhesive.
For achieving the above object, the present invention provides following technical scheme：
1st, the adhesive prepared using giant salamander mucus, described adhesive is the freeze-dried powder or vacuum drying powder of giant salamander mucus.
2nd, the preparation method of described adhesive, comprises the following steps：Giant salamander mucus is frozen into solid, then freezing vacuum
Dry and remove moisture removal, then dry giant salamander mucus is crushed, sterilizing is produced.
It is preferred that, the condition of the freezing is freezed in the case where temperature is not higher than -20 DEG C.
It is preferred that, the condition of the freezing is freezed in the case where temperature is -80 DEG C~-20 DEG C.
It is preferred that, the condition of the vacuum freezedrying is less than 20Pa bars in temperature less than or equal to -50 DEG C, vacuum
It is freeze-dried under part at least 48 hours.
The preparation method of the vacuum drying powder comprises the following steps：Giant salamander mucus is not higher than to 37 DEG C of condition in temperature
Lower vacuum drying, then dry giant salamander mucus is crushed, sterilizing is produced.
It is preferred that, the crushing is that ultramicro grinding to particle diameter is 300~1000 mesh, and the temperature of ultramicro grinding is less than 37 DEG C i.e.
Can, add liquid nitrogen and carry out ultramicro grinding best results.
It is preferred that, the sterilizing is 2~5 × 104The 60Co gamma-rays radiation sterilizations of Gy dosage.
The beneficial effects of the present invention are：The invention discloses the adhesive prepared using giant salamander mucus, the adhesive
Bond properties is strong, good biocompatibility, degradable, and security is good, can act as tissue adhesive, as operation stitching etc.；
The invention also discloses the preparation method of adhesive, preparation method is simple, it is not necessary to special installation, and giant salamander can be kept to glue
The activity of liquid, is easy to industrialization to mass produce.
Brief description of the drawings
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, the present invention provides drawings described below：
Fig. 1 is the bonding strength measurement result of different tissues adhesive.
Fig. 2 is that tissue adhesive bonds experiment and tradition suture comparing result (A in vitro：Tissue adhesive bonds reality in vitro
Test；B：Tradition suture result).
Fig. 3 is 14 days wound healing situation (A of Post operation：Tissue adhesive bonds experiment in vitro；B：Tradition suture result).
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.It is unreceipted specific in embodiment
The experimental method of condition, generally according to normal condition or according to the condition proposed by manufacturer.
The collection of giant salamander mucus directly scrapes sassafras method using skin：Fresh and alive giant salamander is specially cleaned into body surface with clear water, removed
The impurity such as cast off a skin, be subsequently placed in dry box, giant salamander body surface is scraped with flat, until giant salamander body surface secretes a large amount of milkys
Mucus, scraping was collected in culture dish, made mucus thickness about 1cm in culture dish.The collection of giant salamander mucus can also use existing
Electrostimulation or etherization method collection, specific method see Publication No. 104223121A and 104206502A China specially
Profit.
Embodiment 1
The method that adhesive is prepared using giant salamander mucus, is comprised the following steps：The culture dish for collecting giant salamander mucus is put -80
DEG C overnight it is frozen into solid, then with vacuum freeze drier vacuum be 19pa, temperature be -50 DEG C at freezing vacuum
Dry 48 hours；Micronizer of the temperature less than 37 DEG C is placed in after lyophilized giant salamander mucus is smashed to pieces again and is crushed to 300 mesh, most
Afterwards with 5 × 104Gy dosage 60Co gamma-rays radiation sterilizations, obtain tissue adhesive, aseptic packaging is standby.
Embodiment 2
The method that adhesive is prepared using giant salamander mucus, is comprised the following steps：The culture dish for collecting giant salamander mucus is put -20
DEG C overnight it is frozen into solid, then with vacuum freeze drier vacuum be 10pa, temperature be -70 DEG C at freezing vacuum
Dry 56 hours；Smash lyophilized giant salamander mucus micronizer of the rearmounted temperature less than 37 DEG C to pieces again and be crushed to 500 mesh, finally
With 2 × 104Gy dosage 60Co gamma-rays radiation sterilizations, obtain tissue adhesive, aseptic packaging is standby.
Embodiment 3
The method that adhesive is prepared using giant salamander mucus, is comprised the following steps：The culture dish for collecting giant salamander mucus is direct
Put in vacuum drying chamber, 48h is dried under 37 DEG C of vacuum, then smash dry giant salamander mucus to pieces rearmounted temperature surpassing less than 37 DEG C
Atomizer is crushed to 1000 mesh, finally with 2 × 104Gy dosage 60Co gamma-rays radiation sterilizations, obtain tissue adhesive, no bacterium bag
Equipment is used.
Embodiment 4
The method that adhesive is prepared using giant salamander mucus, is comprised the following steps：The culture dish for collecting giant salamander mucus is direct
Put in vacuum drying chamber, 48h is dried under 20 DEG C of vacuum, then smash dry giant salamander mucus to pieces rearmounted temperature surpassing less than 37 DEG C
Atomizer is crushed to 800 mesh, finally with 2 × 104Gy dosage 60Co gamma-rays radiation sterilizations, obtain tissue adhesive, no bacterium bag
Equipment is used.
Embodiment 5
External bonding experiment is carried out to adhesive made from above-described embodiment 1~4, it is specific as follows：Tried using hydraulic universal
Test machine (UTM) and determine adhesive to the cohesive of pigskin, comprise the following steps that：Pig skin of back is cleaned up, take 2cm ×
2 pieces of 10cm skins, remove subcutaneous fat, then in 1 piece of skin medial surface one end 2cm × 2cm area even spreads embodiment 1~4
Obtained adhesive 100mg, another piece of skin inner opposite end 2cm × 2cm area presses with finger bonding 30 seconds with the skin, so
After detect bonding strength, while using Fibrin Glue and cyanoacrylate as control, as a result as shown in table 1 and Fig. 1.As a result
It has been shown that, the bonding strength average out to 0.5MPa of obtained adhesive, bonding strength is far above the bonding strength of Fibrin Glue,
But less than cyanoacrylate.
Table 1, the bonding strength of different adhesives are determined
| Adhesive | Composition | Bonding strength (Mpa) |
| Giant salamander mucus adhesive | Giant salamander mucus | 0.500±0.150 |
| Fibrin Glue | Fibrinogen 5wt% | 0.025±0.003 |
| Cyanoacrylate | Cyanoacrylate | 0.710±0.135 |
It can be seen from the results above that adhesive adhesive strength produced by the present invention is high, adhesive can act as.Due to this
Adhesive source is natural, and security is good, therefore can be used as tissue adhesive.
Embodiment 6
External bonding experiment is carried out to adhesive made from above-described embodiment 1~4, is specially：Using Kunming (KM) mouse as
Experimental animal, takes KM mouse 18,20 ± 4g of body weight.Mouse one day before surgery is anaesthetized, shaving, depilation, second day with small
Mouse mass fraction is anaesthetized for 3% yellow Jackets, with Iodophor, the ethanol disinfection operative site that volume fraction is 70%, hand
Art knife is experimental group doing skin incision 2cm, left side respectively by the left and right sides of boundary of back center backbone, and right side is control
Group.Experimental group is bonded using adhesive made from embodiment 1~4 to wound, and lower embodiment 1~4 is spread in wound joint
Obtained adhesive 5mg, is then gently drawn with tweezers in interface both sides, as a result as shown in A in Fig. 2.As a result show, it is viscous through adhesive
The visible dermis surface of a wound has been bonded together after conjunction, and surface of a wound covering sterilizing petrolatum gauze, last outside elastoplast encloses chest and abdomen
Portion's wrapping one is enclosed.Control group docks skin original position, and tradition 4 pins of suture are carried out with gutstring, and the surface of a wound covers sterilizing petrolatum gauze,
Last outside elastoplast encloses thorax abdomen and wraps up a circle, as shown in B in Fig. 2.Then 6 mouse sights were taken respectively in postoperative 14 days
Wound healing situation is examined, as a result as shown in Figure 3.
From the figure 3, it may be seen that in situ can be healed using the mouse and tradition suture mouse wound of invention adhesives, but tradition
Suture mouse healing time slower, and inflammatory reaction is substantially, scar is obvious.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment the present invention is described in detail, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (8)
1. the adhesive prepared using giant salamander mucus, it is characterised in that：Described adhesive is the freeze-dried powder or vacuum of giant salamander mucus
Xeraphium；
The preparation method of the freeze-dried powder comprises the following steps：Giant salamander mucus is frozen into solid, then vacuum freezedrying is gone
Moisture removal, then dry giant salamander mucus is crushed, sterilizing is produced；
The preparation method of the vacuum drying powder comprises the following steps：By giant salamander mucus under conditions of temperature is not higher than 37 DEG C it is true
Sky is dried, then dry giant salamander mucus is crushed, and sterilizing is produced.
2. the preparation method of claim 1 described adhesive, it is characterised in that the preparation method of the freeze-dried powder includes following step
Suddenly：Giant salamander mucus is frozen into solid, then vacuum freezedrying removes moisture removal, then dry giant salamander mucus is crushed, sterilizing,
Produce.
3. preparation method according to claim 2, it is characterised in that：The condition of the freezing is to be not higher than -20 in temperature
Freezed at DEG C.
4. preparation method according to claim 3, it is characterised in that the condition of the freezing be temperature be -80 DEG C ~ -
Freezed at 20 DEG C.
5. preparation method according to claim 2, it is characterised in that the condition of the vacuum freezedrying is small in temperature
In or equal to -50 DEG C, vacuum be less than 20Pa under the conditions of be freeze-dried at least 48 hours.
6. the preparation method of adhesive according to claim 1, it is characterised in that the preparation method bag of the vacuum drying powder
Include following steps：Giant salamander mucus is dried in vacuo under conditions of temperature is not higher than 37 DEG C, then dry giant salamander mucus is crushed,
Sterilizing, is produced.
7. the preparation method according to any one of claim 2 ~ 6, it is characterised in that the crushing is to be less than 37 DEG C in temperature
Lower ultramicro grinding to particle diameter is 300 ~ 1000 mesh.
8. the preparation method according to any one of claim 2 ~ 6, it is characterised in that the sterilizing is 2 ~ 5 × 104Gy dosage
60Co gamma-rays radiation sterilizations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510154685.XA CN104815349B (en) | 2015-04-02 | 2015-04-02 | Adhesive prepared using giant salamander mucus and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510154685.XA CN104815349B (en) | 2015-04-02 | 2015-04-02 | Adhesive prepared using giant salamander mucus and preparation method thereof |
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| Publication Number | Publication Date |
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| CN104815349A CN104815349A (en) | 2015-08-05 |
| CN104815349B true CN104815349B (en) | 2017-10-24 |
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| CN201510154685.XA Expired - Fee Related CN104815349B (en) | 2015-04-02 | 2015-04-02 | Adhesive prepared using giant salamander mucus and preparation method thereof |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106511399B (en) * | 2016-11-16 | 2023-12-01 | 太鲵生物科技（天津）有限公司 | Application of giant salamander mucus |
| CN108066369A (en) * | 2016-11-16 | 2018-05-25 | 太鲵生物科技（天津）有限公司 | A kind of preparation method of giant salamander pituitary extract |
| CN106581736B (en) * | 2016-12-28 | 2020-04-21 | 安顺市四海农业科技开发有限公司 | Medical adhesive taking giant salamander body surface mucus as raw material and preparation method thereof |
| CN107412845B (en) * | 2017-08-10 | 2020-09-11 | 魏泓 | Giant salamander secretion biomembrane and preparation method and application thereof |
| US20220267655A1 (en) * | 2019-02-26 | 2022-08-25 | Stomatological Hospital Of Chongqing Medical University | Medical adhesive and the preparation method and use thereof |
| CN110559469B (en) * | 2019-02-26 | 2021-08-03 | 重庆医科大学附属口腔医院 | Medical adhesive |
| CN112891380B (en) * | 2021-01-13 | 2023-03-10 | 金华市鼎鑫大鲵驯养繁殖场 | Extraction method of giant salamander mucus and grease and pharmaceutical product thereof |
| CN113209360B (en) * | 2021-03-09 | 2022-02-01 | 吉林大学 | Medical adhesive for promoting wound healing and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102335288A (en) * | 2010-07-20 | 2012-02-01 | 陕西理工学院 | Giant salamander skin mucus scalding ointment |
| CN103829235B (en) * | 2014-01-11 | 2015-07-01 | 陕西理工学院 | Preparation method of giant salamander skin mucus whole powder |
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Inventor after: Shang Haitao Inventor after: Wang Ying Inventor after: Chen Bing Inventor after: Gou Qiang Inventor after: Xing Mengqiu Inventor after: Zhou Minghui Inventor before: Shang Haitao Inventor before: Wang Ying Inventor before: Chen Bing Inventor before: Gou Qiang Inventor before: Wei Hong Inventor before: Xing Mengqiu Inventor before: Zhou Minghui |
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