CN104740678B - Application of the giant salamander mucus in adhesive is prepared - Google Patents
Application of the giant salamander mucus in adhesive is prepared Download PDFInfo
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- CN104740678B CN104740678B CN201510155072.8A CN201510155072A CN104740678B CN 104740678 B CN104740678 B CN 104740678B CN 201510155072 A CN201510155072 A CN 201510155072A CN 104740678 B CN104740678 B CN 104740678B
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- adhesive
- giant salamander
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- 241000157862 Dicamptodontidae Species 0.000 title claims abstract description 32
- 210000003097 mucus Anatomy 0.000 title claims abstract description 30
- 239000000853 adhesive Substances 0.000 title abstract description 48
- 230000001070 adhesive effect Effects 0.000 title abstract description 48
- 239000003106 tissue adhesive Substances 0.000 claims abstract description 21
- 210000000056 organ Anatomy 0.000 claims abstract description 8
- 239000000123 paper Substances 0.000 claims description 11
- 229920001971 elastomer Polymers 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 4
- 239000005060 rubber Substances 0.000 claims description 4
- 239000003292 glue Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 6
- 208000031737 Tissue Adhesions Diseases 0.000 abstract 1
- 206010052428 Wound Diseases 0.000 description 11
- 208000027418 Wounds and injury Diseases 0.000 description 11
- 210000001519 tissue Anatomy 0.000 description 11
- 210000003491 skin Anatomy 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 238000000227 grinding Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 108010080379 Fibrin Tissue Adhesive Proteins 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 229920001651 Cyanoacrylate Polymers 0.000 description 5
- MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000002202 Polyethylene glycol Substances 0.000 description 4
- 238000009455 aseptic packaging Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 229920001223 polyethylene glycol Polymers 0.000 description 4
- 229920002635 polyurethane Polymers 0.000 description 4
- 239000004814 polyurethane Substances 0.000 description 4
- 230000005855 radiation Effects 0.000 description 4
- 238000010008 shearing Methods 0.000 description 4
- 206010061218 Inflammation Diseases 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
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- 231100000241 scar Toxicity 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 239000004264 Petrolatum Substances 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 210000000038 chest Anatomy 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 230000023597 hemostasis Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 229940066842 petrolatum Drugs 0.000 description 2
- 235000019271 petrolatum Nutrition 0.000 description 2
- 230000002980 postoperative effect Effects 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000227633 Ocotea pretiosa Species 0.000 description 1
- 235000004263 Ocotea pretiosa Nutrition 0.000 description 1
- 241000256856 Vespidae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000007767 bonding agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 125000004093 cyano group Chemical group *C#N 0.000 description 1
- RPGQNYWRHVRNGS-UHFFFAOYSA-N cyano prop-2-enoate Chemical compound C(#N)OC(C=C)=O.C(#N)OC(C=C)=O RPGQNYWRHVRNGS-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000035617 depilation Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000001935 peptisation Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000004003 subcutaneous fat Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 239000003356 suture material Substances 0.000 description 1
- 229940075469 tissue adhesives Drugs 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention discloses application of the giant salamander mucus in adhesive is prepared, using giant salamander mucus there is the characteristics of bond properties is strong after freeze-dried powder is made, and because the adhesive derives from giant salamander mucus, for natural component, security is good, and with preferable biocompatibility, it is degradable, tissue adhesive is can act as, is that medically surface of a wound bonding, tissue adhesion, organ bonding etc. provide new material.
Description
Technical field
The invention belongs to technical field of biological material, and in particular to a kind of application of giant salamander mucus in adhesive is prepared.
Background technology
Adhesive is the sticking material of tool, and the material of two kinds of separation can link together by its viscosity.According to original
Material source can be divided into natural glue and artificial adhesive.Tissue adhesive is to refer to polymerize in vivo, thus triggers tissue
Between or tissue with it is non-tissue (such as implant) between bonding, and with control bleed (hemostasis) and prevent gas and liquid flowing
One class biomaterial of functions such as (sealings).Relative to clinical traditional fixation and suture material or apparatus (such as suture, rivet
Deng), tissue adhesive have easy to use, wound it is small, using painful it is small, postoperative need not remove and operative scar is small etc.
Advantage, and can also realize that the fixed point of medicine discharges as the carrier of hemostasis, antibacterial and anti-inflammation drugs, therefore clinically have
There is wide application potential.
Clinically wide variety of tissue adhesive is mainly cyanoacrylate and fibrin at present, but due to
Respective inherent defect can not turn into preferable tissue adhesive.Although the synthetic work Shang Kai of Novel cyano acrylate
Exhibition, but its polymer film is frangible, may be shifted in active organization, and large area wound is dfficult to apply to, it is particularly it
Unfavorable tissue reaction and cause the tumour of experimental animal to limit it further to apply.Fibrin adhesive curing is slow, machinery
Intensity difference, and with the danger of virus infection, and can not be with body tissue covalent bond.
In addition, gelatin (GRF or GRFG), polyethylene glycol (PEG) hydrogel, polyurethane binder
Etc. (polyurethanes) also there are certain application in other tissue adhesives.Gelatin (be derived from organism) for a class gelatin-
The tissue adhesive that benzenediol-formalin or gelatin-glutaraldehyde are made, although it provides high adherence, formalin
Or glutaraldehyde can carry out cross-linking reaction with the protein of target tissue, produce tissue toxicity.Polyethylene glycol (PEG) water-setting peptization it is swollen
Rate is more than 400%, and its cohesive needs relatively dry surface.Make in polyurethane binder (polyurethanes)
Material aromatic diisocyanate is poisonous to organism.
Therefore, be badly in need of providing a kind of natural biological sources and quantity it is sufficient, good biocompatibility, adhesive strength it is high, can
Biodegradable tissue adhesive.
The content of the invention
In view of this, the application it is an object of the invention to provide giant salamander mucus in tissue adhesive is prepared, is developed
The new application of giant salamander mucus, adhesive field is not there is provided new material.
For achieving the above object, the present invention provides following technical scheme：
1st, application of the giant salamander mucus in adhesive is prepared.
It is preferred that, described adhesive is tissue adhesive.
It is furthermore preferred that the tissue adhesive is used for the surface of a wound, tissue, organ, rubber, metal or paper bonding.
The beneficial effects of the present invention are：The invention discloses application of the giant salamander mucus in adhesive is prepared, the bonding
Agent derives from giant salamander mucus, and composition is natural, and security is good, and bonding strength is high, can be used as tissue adhesive or other bondings
Agent, for the bonding such as the surface of a wound, tissue, organ, rubber, metal or paper, with wound healing time faster, inflammatory reaction it is light, scar
The advantages of trace is small, is with a wide range of applications.
Brief description of the drawings
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, the present invention provides drawings described below：
Fig. 1 is the bonding strength measurement result of different adhesives.
Fig. 2 is that adhesive bonds experiment and tradition suture comparing result (A in vitro：Adhesive bonds experiment in vitro；B：Tradition
Suture result).
Fig. 3 is Post operation different time healing state (A：Adhesive bonds experimental result in vitro；B tradition sutures result).
Fig. 4 is vitro in organ's adhesion characteristic result (A of adhesive：Kidney adhesion results；B：Heart adhesion results).
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.It is unreceipted specific in embodiment
The experimental method of condition, generally according to normal condition or according to the condition proposed by manufacturer.
The collection of giant salamander mucus directly scrapes sassafras method using skin：Fresh and alive giant salamander is specially cleaned into body surface with clear water, removed
The impurity such as cast off a skin, be subsequently placed in dry box, giant salamander body surface is scraped with flat, until giant salamander body surface secretes a large amount of milkys
Mucus, scraping was collected in culture dish, made mucus thickness about 1cm in culture dish.The collection of giant salamander mucus can also use existing
Electrostimulation or etherization method collection, specific method see Publication No. 104223121A and 104206502A China specially
Profit.
Embodiment 1
The method that adhesive is prepared using giant salamander mucus, is comprised the following steps：The culture dish for collecting giant salamander mucus is put -80
DEG C overnight it is frozen into solid, then with vacuum freeze drier vacuum be 19pa, temperature be -50 DEG C at freezing vacuum
Dry 48 hours；Smash lyophilized giant salamander mucus to pieces the rearmounted broken machine of temperature low-temperature submicron powder again and be crushed to 300 mesh, finally with 5 ×
104Gy dosage 60Co gamma-rays radiation sterilizations, obtain tissue adhesive, aseptic packaging is standby.The temperature of wherein low-temperature grinding is less than
37 DEG C, add liquid nitrogen ultramicro grinding best results.
Embodiment 2
The method that adhesive is prepared using giant salamander mucus, is comprised the following steps：The culture dish for collecting giant salamander mucus is put -20
DEG C overnight it is frozen into solid, then with vacuum freeze drier vacuum be 10pa, temperature be -70 DEG C at freezing vacuum
Dry 56 hours；Smash lyophilized giant salamander mucus to pieces rearmounted low-temperature submicron powder broken machine again and be crushed to 500 mesh, finally with 5 × 104Gy
Dosage 60Co gamma-rays radiation sterilizations, obtain tissue adhesive, aseptic packaging is standby.Wherein the temperature of low-temperature grinding is less than 37 DEG C
Can, add liquid nitrogen ultramicro grinding best results.
Embodiment 3
The method that adhesive is prepared using giant salamander mucus, is comprised the following steps：The culture dish for collecting giant salamander mucus is direct
Put in vacuum drying chamber, 48h is dried under 37 DEG C of vacuum, then smash dry giant salamander mucus to pieces rearmounted low-temperature submicron powder broken machine powder
1000 mesh are broken to, finally with 2 × 104Gy dosage 60Co gamma-rays radiation sterilizations, obtain tissue adhesive, aseptic packaging is standby.Its
The temperature of middle low-temperature grinding is less than 37 DEG C, adds liquid nitrogen ultramicro grinding best results.
Embodiment 4
The method that adhesive is prepared using giant salamander mucus, is comprised the following steps：The culture dish for collecting giant salamander mucus is direct
Put in vacuum drying chamber, 48h is dried under 20 DEG C of vacuum, then smash dry giant salamander mucus to pieces rearmounted low-temperature submicron powder broken machine powder
800 mesh are broken to, finally with 2 × 104Gy dosage 60Co gamma-rays radiation sterilizations, obtain tissue adhesive, aseptic packaging is standby.Wherein
The temperature of low-temperature grinding is less than 37 DEG C, adds liquid nitrogen ultramicro grinding best results.
Embodiment 5
Skin is carried out to adhesive made from above-described embodiment 1~4 and bonds experiment in vitro, it is specific as follows：Use hydraulic pressure ten thousand
Energy testing machine (UTM) determines tissue adhesive to the cohesive of pigskin, comprises the following steps that：Pig skin of back is cleaned up,
2 pieces of 2cm × 10cm skins are taken, subcutaneous fat is removed, then implemented in 1 piece of skin medial surface one end 2cm × 2cm areas even spread
Adhesive 100mg made from example 1~4, another piece of skin inner opposite end 2cm × 2cm area presses with finger bonding with the skin
30 seconds, bonding strength is then detected, while using Fibrin Glue and cyanoacrylate as control, as a result such as table 1 and Fig. 1 institutes
Show.As a result show, the bonding strength average out to 0.5MPa of obtained adhesive, the bonding strength is viscous far above Fibrin Glue
Intensity is closed, but less than cyanoacrylate.
Table 1, the bonding strength of different tissues adhesive are determined
| Adhesive | Composition | Bonding strength (Mpa) |
| Giant salamander mucus adhesive | Giant salamander mucus freeze-dried products | 0.500±0.150 |
| Fibrin Glue | Fibrinogen 5wt% | 0.025±0.003 |
| Cyanoacrylate | Cyanoacrylate | 0.710±0.135 |
It can be seen from the results above that adhesive adhesive strength produced by the present invention is high, adhesive can act as.And by
In ingredient origin in giant salamander, composition is pure natural, and security is good, degradable, therefore can be used as tissue adhesive.
Embodiment 6
Surface of a wound bonding experiment is carried out to adhesive made from above-described embodiment 1~4, is specially：Using Kunming (KM) mouse as
Experimental animal, takes KM mouse 18,20 ± 4g of body weight.Mouse one day before surgery is anaesthetized, shaving, depilation, second day with small
Mouse mass fraction is anaesthetized for 3% yellow Jackets, with Iodophor, the ethanol disinfection operative site that volume fraction is 70%, hand
Art knife is experimental group doing skin incision 2cm, left side respectively by the left and right sides of boundary of back center backbone, and right side is control
Group.Experimental group is bonded using adhesive made from embodiment 1~4 to wound, and lower embodiment 1~4 is spread in wound joint
Obtained adhesive 5mg, is then gently drawn with tweezers in interface both sides, as a result as shown in A in Fig. 2.As a result show, it is viscous through adhesive
The visible dermis surface of a wound has been bonded together after conjunction, and surface of a wound covering sterilizing petrolatum gauze, last outside elastoplast encloses chest and abdomen
Portion's wrapping one is enclosed.Control group docks skin original position, and tradition 4 pins of suture are carried out with gutstring, and the surface of a wound covers sterilizing petrolatum gauze,
Last outside elastoplast encloses thorax abdomen and wraps up a circle, as shown in B in Fig. 2.Then 6 mouse sights were taken respectively in postoperative 14 days
Wound healing situation is examined, as a result as shown in Figure 3.
From the figure 3, it may be seen that in situ can be healed using the mouse and tradition suture mouse wound of invention adhesives, but tradition
Suture mouse healing time slower, and inflammatory reaction is substantially, scar is obvious.
Embodiment 7
Organ is carried out to adhesive made from above-described embodiment 1~4 and bonds experiment in vitro, it is specific as follows：Use hydraulic pressure ten thousand
Energy testing machine (UTM) determines cohesive of the tissue adhesive to pig organ otch, and specific method is as follows：Pig part kidney is taken respectively
And heart, the transverse incision more than 1/2 section is made of scalpel, in adhesive made from tangent plane side even spread embodiment 1~4
200-500mg, finger pressing bonding in situ 30 seconds, then detects bonding strength (Fig. 4), while with Fibrin Glue and cyano group third
Olefin(e) acid ester can good bond as control, as a result kidney cutting part.The bonding strength average out to 0.5MPa of obtained adhesive,
The bonding strength is far above the bonding strength of Fibrin Glue, but less than cyanoacrylate.
Embodiment 8
Hydroxyapatite powder 5g or so is taken, adhesive 100-300mg made from above-described embodiment 1~4 is added, added double
Water 200-800 μ L are steamed, are stirred, globulate is extruded, after natural drying, can bond as a globular solids.
Embodiment 9
2 pieces of coins are taken, respectively take one side to be bedewed with water, wherein made from one piece of coin side even spread embodiment 1~4
Then 2 pieces of coins are bedewed side overlapping about 1/2, finger pressing bonding in situ 30 seconds, then detection bonding by adhesive 100mg
Intensity.As a result 19 ± 5Jm of overlapping shearing stress average out to of obtained adhesive is shown-2。
Embodiment 10
2cm × 15cm filter paper bar 2 is made with ordinary filter paper, and 1 filter paper one end 2cm places are added dropwise 1 and dripped about 100 μ L, filter
Paper is dripped, and water suction is got wet rapidly at place, diffusion area about 2cm × 2cm, in adhesive made from wet place's even spread embodiment 1~4
50mg, it is then that another 1 filter paper one end is overlapping with the wet place of the filter paper that drips (2cm × 2cm), after natural drying, detect bonding strength.
As a result 23 ± 5Jm of overlapping shearing stress average out to of obtained adhesive is shown-2。
Embodiment 11
2cm × 15cm latexes bar 2 is made with emgloves, and 1 filter paper one end 2cm places dropwise addition 1 is dripped about 100 μ L,
Adhesive 50mg made from even spread embodiment 1~4 at water droplet, then by another 1 latex bar one end and adhesive coating
Overlapping (2cm × 2cm), after natural drying, detects overlapping shearing stress (lap-shear), as a result shows the weight of obtained adhesive
25 ± 5Jm of folded shearing stress average out to-2。
The above results, which are indicated, is had good bond properties by adhesive produced by the present invention, can be used as adhesive
In the bonding of tissue, organ, metal, rubber, paper, powdered substance etc..And bond properties is good, and application prospect is extensive.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment the present invention is described in detail, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (2)
1. application of the giant salamander mucus in tissue adhesive is prepared.
2. application according to claim 1, it is characterised in that：The tissue adhesive is used for the surface of a wound, tissue, organ, rubber
Glue, metal or paper bonding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510155072.8A CN104740678B (en) | 2015-04-02 | 2015-04-02 | Application of the giant salamander mucus in adhesive is prepared |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510155072.8A CN104740678B (en) | 2015-04-02 | 2015-04-02 | Application of the giant salamander mucus in adhesive is prepared |
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| CN104740678A CN104740678A (en) | 2015-07-01 |
| CN104740678B true CN104740678B (en) | 2017-10-24 |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108066369A (en) * | 2016-11-16 | 2018-05-25 | 太鲵生物科技（天津）有限公司 | A kind of preparation method of giant salamander pituitary extract |
| CN106361776B (en) * | 2016-11-16 | 2024-03-19 | 张家界金姑大鲵开发有限公司 | Giant salamander mucus used for hemostasis |
| CN106581736B (en) * | 2016-12-28 | 2020-04-21 | 安顺市四海农业科技开发有限公司 | Medical adhesive taking giant salamander body surface mucus as raw material and preparation method thereof |
| CN106693039B (en) * | 2017-01-24 | 2019-10-01 | 南开大学 | A kind of preparation method of the medical aquogel with good biological adhesiveness |
| CN107412845B (en) * | 2017-08-10 | 2020-09-11 | 魏泓 | Giant salamander secretion biomembrane and preparation method and application thereof |
| CN107456599B (en) * | 2017-08-10 | 2020-09-01 | 魏泓 | Antibacterial giant salamander secretion biomembrane and preparation method and application thereof |
| CN107279508A (en) * | 2017-08-15 | 2017-10-24 | 正安县正鲵养殖农民专业合作社 | Chinese medicine fodder for cultivating giant salamander |
| US20220267655A1 (en) * | 2019-02-26 | 2022-08-25 | Stomatological Hospital Of Chongqing Medical University | Medical adhesive and the preparation method and use thereof |
| CN110559469B (en) * | 2019-02-26 | 2021-08-03 | 重庆医科大学附属口腔医院 | Medical adhesive |
| CN112891380B (en) * | 2021-01-13 | 2023-03-10 | 金华市鼎鑫大鲵驯养繁殖场 | Extraction method of giant salamander mucus and grease and pharmaceutical product thereof |
| CN116077717B (en) * | 2021-11-05 | 2024-01-30 | 重庆医科大学附属口腔医院 | Bioburden system for wound healing and application thereof |
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| CN102335288A (en) * | 2010-07-20 | 2012-02-01 | 陕西理工学院 | Giant salamander skin mucus scalding ointment |
| CN103829235B (en) * | 2014-01-11 | 2015-07-01 | 陕西理工学院 | Preparation method of giant salamander skin mucus whole powder |
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